Rroxscaffold_4G00287640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001015
Physical Location & Seq
Reverse (-)
8637881 .. 8638357
477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287640.1

Sequence Viewer

Length: 477 bp
ATGGCGTTACTATCAAAACTTTATTCTGGACTCCATAATCTCAAAAACTTATGCATATACAGCTCCGCAAATATGAAAACAAACCCTGAGAGCAGCCACCGTTCTGCTTACACCGACGGTTTCCGGAGATCCTCGGCACTGATGAAGAAGATGAAGATGAAAGAGAAAGTTTTGGAAAAGAAAGTGCATGTTAAGTCCTTGAAGATTTGGATCCAAGAAGTCGTGGGAATTGACCCCCCAAGTCGCAGCATCCTACCTGACTCTGAACGGGTCTACCAAATCGTGTGCCGGGTTGATGGACCCCAAGAAATAGAAGTCGACGCCTTTTATGGAAAGGCAAGGGGACCAAATCCTTCATGGTTCAAATATACTACAGTTCCTTTGGATGGCCACCCCTTAGGGGAGGGCTTTCTGAACGTGGAGGTATTGAGGTTTAATAGTAAGGAAAACCTCACCGACCCTGGAACCTCCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.84

Weight (kDa)

9.18

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 273, 318
AccIII TCCGGA 1 cut(s) 123
AciI CCGC 1 cut(s) 66
AclWI GGATC 3 cut(s) 123, 205, 218
AcoI YGGCCR 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 321
AfiI CCNNNNNNNGG 2 cut(s) 386, 400
AgsI TTSAA 2 cut(s) 202, 364
AjnI CCWGG 1 cut(s) 460
AluBI AGCT 1 cut(s) 63
AluI AGCT 1 cut(s) 63
AlwI GGATC 3 cut(s) 123, 205, 218
Aor13HI TCCGGA 1 cut(s) 123
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 2 cut(s) 93, 246
AspS9I GGNCC 2 cut(s) 299, 344
AsuC2I CCSGG 1 cut(s) 290
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 2 cut(s) 299, 344
AxyI CCTNAGG 1 cut(s) 397
BalI TGGCCA 1 cut(s) 390
BamHI GGATCC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 105, 258
BccI CCATC 2 cut(s) 290, 380
BciT130I CCWGG 1 cut(s) 462
BcnI CCSGG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 2 cut(s) 94, 247
BlsI GCNGC 2 cut(s) 95, 248
Bme1390I CCNGG 2 cut(s) 290, 462
Bme18I GGWCC 2 cut(s) 299, 344
BmgT120I GGNCC 2 cut(s) 299, 344
BmiI GGNNCC 4 cut(s) 212, 301, 345, 466
BmrFI CCNGG 2 cut(s) 290, 462
BmsI GCATC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 132, 460
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 400
Bse21I CCTNAGG 1 cut(s) 397
Bse8I GATNNNNATC 1 cut(s) 209
BseAI TCCGGA 1 cut(s) 123
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 132, 460
BseGI GGATG 2 cut(s) 249, 391
BseJI GATNNNNATC 1 cut(s) 209
BseLI CCNNNNNNNGG 2 cut(s) 386, 400
BseMII CTCAG 1 cut(s) 78
BseXI GCAGC 2 cut(s) 105, 258
BshFI GGCC 1 cut(s) 390
BsiSI CCGG 2 cut(s) 124, 289
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 386, 400
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 1 cut(s) 390
Bsp13I TCCGGA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 128, 210
BspACI CCGC 1 cut(s) 66
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 1 cut(s) 79
BspEI TCCGGA 1 cut(s) 123
BspLI GGNNCC 4 cut(s) 212, 301, 345, 466
BspPI GGATC 3 cut(s) 123, 205, 218
BssECI CCNNGG 2 cut(s) 132, 460
BssMI GATC 2 cut(s) 128, 210
BssNI GRCGYC 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 3 cut(s) 101, 119, 376
BstACI GRCGYC 1 cut(s) 321
BstDEI CTNAG 2 cut(s) 87, 397
BstF5I GGATG 2 cut(s) 249, 391
BstKTI GATC 2 cut(s) 131, 213
BstMBI GATC 2 cut(s) 128, 210
BstMWI GCNNNNNNNGC 1 cut(s) 60
BstNI CCWGG 1 cut(s) 462
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 2 cut(s) 288, 460
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 105, 258
BstX2I RGATCY 2 cut(s) 128, 210
BstYI RGATCY 2 cut(s) 128, 210
Bsu36I CCTNAGG 1 cut(s) 397
BsuRI GGCC 1 cut(s) 390
BtsCI GGATG 2 cut(s) 249, 391
BtsIMutI CAGTG 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 299, 344
CseI GACGC 1 cut(s) 329
CviAII CATG 2 cut(s) 188, 357
CviJI RGCY 4 cut(s) 63, 96, 390, 408
CviKI_1 RGCY 4 cut(s) 63, 96, 390, 408
DdeI CTNAG 2 cut(s) 87, 397
DpnI GATC 2 cut(s) 130, 212
DpnII GATC 2 cut(s) 128, 210
EaeI YGGCCR 1 cut(s) 388
Eco47I GGWCC 2 cut(s) 299, 344
Eco81I CCTNAGG 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 460
EcoT22I ATGCAT 1 cut(s) 56
FaeI CATG 2 cut(s) 191, 360
FaiI YATR 9 cut(s) 36, 52, 56, 58, 74, 189, 330, 358, 369
FaqI GGGAC 1 cut(s) 357
FatI CATG 2 cut(s) 187, 356
FblI GTMKAC 2 cut(s) 273, 318
Fnu4HI GCNGC 2 cut(s) 94, 247
FokI GGATG 2 cut(s) 236, 398
Fsp4HI GCNGC 2 cut(s) 94, 247
GluI GCNGC 2 cut(s) 94, 247
HaeIII GGCC 1 cut(s) 390
HapII CCGG 2 cut(s) 124, 289
HgaI GACGC 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 2 cut(s) 191, 360
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 2 cut(s) 30, 260
HpaII CCGG 2 cut(s) 124, 289
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 2 cut(s) 274, 319
Hpy188I TCNGA 3 cut(s) 265, 414, 472
Hpy188III TCNNGA 2 cut(s) 27, 124
Hpy8I GTNNAC 2 cut(s) 274, 319
Hpy99I CGWCG 2 cut(s) 119, 323
HpyAV CCTTC 1 cut(s) 363
HpyCH4III ACNGT 3 cut(s) 101, 119, 376
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 2 cut(s) 54, 187
HpyF10VI GCNNNNNNNGC 1 cut(s) 60
HpyF3I CTNAG 2 cut(s) 87, 397
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 2 cut(s) 191, 360
Kpn2I TCCGGA 1 cut(s) 123
Kzo9I GATC 2 cut(s) 128, 210
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 6 cut(s) 12, 99, 137, 270, 302, 447
Lsp1109I GCAGC 2 cut(s) 105, 258
LweI GCATC 1 cut(s) 258
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 6
MalI GATC 2 cut(s) 130, 212
MboI GATC 2 cut(s) 128, 210
MboII GAAGA 4 cut(s) 157, 160, 166, 214
MflI RGATCY 2 cut(s) 128, 210
MlsI TGGCCA 1 cut(s) 390
MluCI AATT 1 cut(s) 228
MluNI TGGCCA 1 cut(s) 390
MlyI GAGTC 2 cut(s) 24, 254
MnlI CCTC 5 cut(s) 142, 397, 415, 423, 461
Mox20I TGGCCA 1 cut(s) 390
Mph1103I ATGCAT 1 cut(s) 56
MroI TCCGGA 1 cut(s) 123
MscI TGGCCA 1 cut(s) 390
MseI TTAA 2 cut(s) 192, 435
Msp20I TGGCCA 1 cut(s) 390
MspI CCGG 2 cut(s) 124, 289
MspR9I CCNGG 2 cut(s) 290, 462
MvaI CCWGG 1 cut(s) 462
MwoI GCNNNNNNNGC 1 cut(s) 60
NciI CCSGG 1 cut(s) 290
NdeII GATC 2 cut(s) 128, 210
NlaIII CATG 2 cut(s) 191, 360
NlaIV GGNNCC 4 cut(s) 212, 301, 345, 466
NmeAIII GCCGAG 1 cut(s) 113
NsiI ATGCAT 1 cut(s) 56
NspI RCATGY 1 cut(s) 191
PkrI GCNGC 2 cut(s) 95, 248
PleI GAGTC 2 cut(s) 24, 254
PpsI GAGTC 2 cut(s) 24, 254
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 4 cut(s) 212, 301, 345, 466
PspPI GGNCC 2 cut(s) 299, 344
PsuI RGATCY 2 cut(s) 128, 210
SalI GTCGAC 1 cut(s) 317
SaqAI TTAA 2 cut(s) 192, 435
SatI GCNGC 2 cut(s) 94, 247
Sau3AI GATC 2 cut(s) 128, 210
Sau96I GGNCC 2 cut(s) 299, 344
SchI GAGTC 2 cut(s) 24, 254
ScrFI CCNGG 2 cut(s) 290, 462
SetI ASST 7 cut(s) 65, 259, 420, 426, 434, 453, 470
SfaNI GCATC 1 cut(s) 258
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 2 cut(s) 299, 344
Sse9I AATT 1 cut(s) 228
SsiI CCGC 1 cut(s) 66
StyD4I CCNGG 2 cut(s) 288, 460
TaaI ACNGT 3 cut(s) 101, 119, 376
TaiI ACGT 1 cut(s) 420
TaqI TCGA 1 cut(s) 318
TasI AATT 1 cut(s) 228
Tru1I TTAA 2 cut(s) 192, 435
Tru9I TTAA 2 cut(s) 192, 435
TscAI CASTG 1 cut(s) 144
TseI GCWGC 2 cut(s) 93, 246
TspDTI ATGAA 5 cut(s) 89, 158, 167, 173, 345
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 2 cut(s) 299, 344
XceI RCATGY 1 cut(s) 191
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmiI GTMKAC 2 cut(s) 273, 318
Zsp2I ATGCAT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.