MD07G1214700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001015
Physical Location & Seq
Forward (+)
29204705 .. 29205775
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1214700.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGCCCCAACAGAAATTCAATCCCTTCTCTCCTTCTTGCCCATTATCCTACGCACCCTCAGCACTCTCCCCACTCGCGCACGTTAGCACAGAGACGCCTAACCTCATGGCTTCCTCCCGCCCTCCGCCGCAGAAGCCTCTCGATCTCGACCTCACGATCGTCTCCGCCAAGCATCTCAAGAACGTCAACTGGAAGAACGGCGACCTCAAGCCCTACGCGGTCTTCTGGGTGGACCCCGACCGCCGGCTGGCCACCAAGTCTGACGACTCCGGCTCCACCCGCCCGGTCTGGAACGAGCGGTTCACCGTCCCCCTCACCCTCTCCCTCCACCACTCGTTCCTCACCCTCGAAATCTTCCACTCCAAACCCTCCGACACTCCCAAGCCCTTAGTTGGCACCCTCCGGGTCCCACTCAAGGACCTGCCAAATCAGGACGACTCGACCCGAATCCGGACCTTCCAGCTCGTCCGTCCCTCCGGCCGTCCCCAGGGCAAGATCCGCGTAAAGCTCGCCGTTCGAGAGCGACCCCTGCCGCCACCGCCAGATTACCATATTACCCCTCCTCCTAGCTATTTTTACGGTGGTGCCCCTATGCCCCCACCGCCTGTTCGCGACTTCAGGTCATACTCGCCGTCGCCTTACATCTCTACTTTACAAGCTTCGGCTCCTAGTCCATCGCCTTCGCCACCGCCTCCGCCTTACCATTACAGCTCCTATTCCGATCAGTACTCGAGTTACTATCCAGCGTACTACTCCAGCGCGCCACCGCCTCCACCAAGGCCGTTCTTCGACCGACCGGTGAACTATGGCGGGCCTAGTGGGAGTGGTGGGAGTGGTGGGCCTGGTGGGCCTTCAGCGCCATTGGACTACTCGAATTATGATCAGAATCAGAAGCCCAAGAGTGGGAAGATGGGATTGGGCACCGGATTAGCTGTGGGCGCAGTGGCCGGAGGTCTTGGTGCACTGGCATTGGATGAGGGGTTAAGGTATGAAGAAGATAAGATGGCGGAGAGGGTCGAGAATGACATGGGTGAGCGTGATGAGTACAGCAATTATCGTGCAGATTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
C2

Protein Analysis

357

Amino Acids

39.02

Weight (kDa)

8.86

Isoelectric Point (pI)

65.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 49 - 147 2e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 429
AccB1I GGYRCC 3 cut(s) 395, 584, 920
AccBSI CCGCTC 1 cut(s) 298
AccII CGCG 5 cut(s) 77, 218, 501, 612, 761
AccIII TCCGGA 1 cut(s) 450
AclWI GGATC 1 cut(s) 490
AcoI YGGCCR 3 cut(s) 249, 478, 945
AcsI RAATTY 1 cut(s) 14
AcuI CTGAAG 2 cut(s) 601, 837
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 3 cut(s) 728, 749, 1046
AfiI CCNNNNNNNGG 8 cut(s) 243, 247, 392, 415, 450, 487, 902, 903
AgeI ACCGGT 1 cut(s) 796
AgsI TTSAA 1 cut(s) 19
AhdI GACNNNNNGTC 1 cut(s) 619
AjnI CCWGG 2 cut(s) 486, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 6 cut(s) 463, 508, 570, 659, 711, 932
AluI AGCT 6 cut(s) 463, 508, 570, 659, 711, 932
Alw21I GWGCWC 1 cut(s) 964
Alw26I GTCTC 2 cut(s) 86, 166
Alw44I GTGCAC 1 cut(s) 960
AlwI GGATC 1 cut(s) 490
Ama87I CYCGRG 1 cut(s) 730
Aor13HI TCCGGA 1 cut(s) 450
AoxI GGCC 7 cut(s) 249, 478, 779, 812, 839, 848, 945
ApaLI GTGCAC 1 cut(s) 960
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 1 cut(s) 796
AspLEI GCGC 5 cut(s) 79, 761, 763, 859, 941
AspS9I GGNCC 7 cut(s) 232, 406, 418, 453, 812, 839, 848
AsuC2I CCSGG 2 cut(s) 284, 404
AsuHPI GGTGA 5 cut(s) 295, 307, 334, 811, 1043
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 4 cut(s) 232, 406, 418, 453
BaeGI GKGCMC 3 cut(s) 589, 923, 964
BalI TGGCCA 1 cut(s) 251
BanI GGYRCC 3 cut(s) 395, 584, 920
BbsI GAAGAC 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 964
BbvCI CCTCAGC 1 cut(s) 58
BccI CCATC 3 cut(s) 682, 904, 997
BceAI ACGGC 5 cut(s) 214, 465, 497, 616, 766
BciT130I CCWGG 2 cut(s) 488, 843
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 2 cut(s) 284, 404
BcoDI GTCTC 2 cut(s) 86, 166
BfaI CTAG 3 cut(s) 567, 669, 816
BfoI RGCGCY 1 cut(s) 860
BfuAI ACCTGC 1 cut(s) 429
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 2 cut(s) 128, 533
BlsI GCNGC 2 cut(s) 129, 534
BmcAI AGTACT 1 cut(s) 728
Bme1390I CCNGG 4 cut(s) 284, 404, 488, 843
Bme18I GGWCC 4 cut(s) 232, 406, 418, 453
BmeRI GACNNNNNGTC 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 7 cut(s) 232, 406, 418, 453, 812, 839, 848
BmiI GGNNCC 8 cut(s) 234, 274, 397, 407, 408, 586, 666, 922
BmrFI CCNGG 4 cut(s) 284, 404, 488, 843
BmsI GCATC 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 214
BpmI CTGGAG 1 cut(s) 739
Bpu10I CCTNAGC 1 cut(s) 58
BpuEI CTTGAG 3 cut(s) 161, 191, 398
BpuMI CCSGG 2 cut(s) 284, 404
BsaBI GATNNNNATC 1 cut(s) 885
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 3 cut(s) 486, 487, 776
BsaWI WCCGGW 3 cut(s) 450, 796, 923
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 547, 577
Bsc4I CCNNNNNNNGG 8 cut(s) 243, 247, 392, 415, 450, 487, 902, 903
Bse118I RCCGGY 2 cut(s) 243, 796
Bse1I ACTGG 2 cut(s) 194, 969
Bse8I GATNNNNATC 1 cut(s) 885
BseAI TCCGGA 1 cut(s) 450
BseBI CCWGG 2 cut(s) 488, 843
BseDI CCNNGG 3 cut(s) 486, 487, 776
BseGI GGATG 1 cut(s) 979
BseJI GATNNNNATC 1 cut(s) 885
BseLI CCNNNNNNNGG 8 cut(s) 243, 247, 392, 415, 450, 487, 902, 903
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 194, 969
BsePI GCGCGC 1 cut(s) 759
BseRI GAGGAG 1 cut(s) 552
BseSI GKGCMC 3 cut(s) 589, 923, 964
BseX3I CGGCCG 1 cut(s) 478
Bsh1236I CGCG 5 cut(s) 77, 218, 501, 612, 761
Bsh1285I CGRYCG 5 cut(s) 159, 241, 481, 793, 797
BshFI GGCC 7 cut(s) 251, 480, 781, 814, 841, 850, 947
BshNI GGYRCC 3 cut(s) 395, 584, 920
BshTI ACCGGT 1 cut(s) 796
BsiEI CGRYCG 5 cut(s) 159, 241, 481, 793, 797
BsiHKAI GWGCWC 1 cut(s) 964
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 9 cut(s) 244, 270, 284, 403, 451, 477, 797, 924, 948
BslFI GGGAC 4 cut(s) 293, 392, 456, 468
BslI CCNNNNNNNGG 8 cut(s) 243, 247, 392, 415, 450, 487, 902, 903
BsmAI GTCTC 2 cut(s) 86, 166
BsmBI CGTCTC 2 cut(s) 86, 166
BsmFI GGGAC 4 cut(s) 293, 392, 456, 468
BsnI GGCC 7 cut(s) 251, 480, 781, 814, 841, 850, 947
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 3 cut(s) 589, 923, 964
Bsp13I TCCGGA 1 cut(s) 450
Bsp143I GATC 5 cut(s) 142, 156, 495, 721, 880
Bsp68I TCGCGA 1 cut(s) 612
BspANI GGCC 7 cut(s) 251, 480, 781, 814, 841, 850, 947
BspCNI CTCAG 1 cut(s) 71
BspEI TCCGGA 1 cut(s) 450
BspFNI CGCG 5 cut(s) 77, 218, 501, 612, 761
BspLI GGNNCC 8 cut(s) 234, 274, 397, 407, 408, 586, 666, 922
BspMI ACCTGC 1 cut(s) 429
BspPI GGATC 1 cut(s) 490
BspT107I GGYRCC 3 cut(s) 395, 584, 920
BsrBI CCGCTC 1 cut(s) 298
BsrFI RCCGGY 2 cut(s) 243, 796
BsrI ACTGG 2 cut(s) 194, 969
BssAI RCCGGY 2 cut(s) 243, 796
BssECI CCNNGG 3 cut(s) 486, 487, 776
BssHII GCGCGC 1 cut(s) 759
BssMI GATC 5 cut(s) 142, 156, 495, 721, 880
BssNI GRCGYC 1 cut(s) 95
BssT1I CCWWGG 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 488, 843
Bst4CI ACNGT 2 cut(s) 307, 581
BstACI GRCGYC 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 245, 249, 510, 761, 812
BstDEI CTNAG 2 cut(s) 58, 388
BstF5I GGATG 1 cut(s) 979
BstFNI CGCG 5 cut(s) 77, 218, 501, 612, 761
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 5 cut(s) 79, 761, 763, 859, 941
BstKTI GATC 5 cut(s) 145, 159, 498, 724, 883
BstMAI GTCTC 2 cut(s) 86, 166
BstMBI GATC 5 cut(s) 142, 156, 495, 721, 880
BstMCI CGRYCG 5 cut(s) 159, 241, 481, 793, 797
BstNI CCWGG 2 cut(s) 488, 843
BstSCI CCNGG 4 cut(s) 282, 402, 486, 841
BstSLI GKGCMC 3 cut(s) 589, 923, 964
BstUI CGCG 5 cut(s) 77, 218, 501, 612, 761
BstV2I GAAGAC 1 cut(s) 214
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
BstZI CGGCCG 1 cut(s) 478
BsuRI GGCC 7 cut(s) 251, 480, 781, 814, 841, 850, 947
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 1 cut(s) 979
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 2 cut(s) 948, 962
BtuMI TCGCGA 1 cut(s) 612
BveI ACCTGC 1 cut(s) 429
Cac8I GCNNGC 5 cut(s) 245, 249, 510, 761, 812
CfoI GCGC 5 cut(s) 79, 761, 763, 859, 941
Cfr10I RCCGGY 2 cut(s) 243, 796
Cfr13I GGNCC 7 cut(s) 232, 406, 418, 453, 812, 839, 848
CseI GACGC 1 cut(s) 103
Csp6I GTAC 3 cut(s) 727, 748, 1045
CspAI ACCGGT 1 cut(s) 796
CviAII CATG 2 cut(s) 106, 1027
CviQI GTAC 3 cut(s) 727, 748, 1045
DdeI CTNAG 2 cut(s) 58, 388
DpnI GATC 5 cut(s) 144, 158, 497, 723, 882
DpnII GATC 5 cut(s) 142, 156, 495, 721, 880
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 3 cut(s) 249, 478, 945
EagI CGGCCG 1 cut(s) 478
Eam1105I GACNNNNNGTC 1 cut(s) 619
EciI GGCGGA 4 cut(s) 114, 154, 684, 1022
EclXI CGGCCG 1 cut(s) 478
Eco130I CCWWGG 1 cut(s) 776
Eco47I GGWCC 4 cut(s) 232, 406, 418, 453
Eco52I CGGCCG 1 cut(s) 478
Eco57I CTGAAG 2 cut(s) 601, 837
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 2 cut(s) 406, 418
EcoRII CCWGG 2 cut(s) 486, 841
EcoT14I CCWWGG 1 cut(s) 776
ErhI CCWWGG 1 cut(s) 776
Esp3I CGTCTC 2 cut(s) 86, 166
FaeI CATG 2 cut(s) 109, 1030
FaiI YATR 8 cut(s) 107, 552, 593, 625, 807, 879, 990, 1028
FaqI GGGAC 4 cut(s) 293, 392, 456, 468
FatI CATG 2 cut(s) 105, 1026
FauI CCCGC 3 cut(s) 125, 287, 803
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 128, 533
FokI GGATG 1 cut(s) 986
Fsp4HI GCNGC 2 cut(s) 128, 533
FspBI CTAG 3 cut(s) 567, 669, 816
GlaI GCGC 5 cut(s) 78, 760, 762, 858, 940
GluI GCNGC 2 cut(s) 128, 533
GsuI CTGGAG 1 cut(s) 739
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 7 cut(s) 251, 480, 781, 814, 841, 850, 947
HapII CCGG 9 cut(s) 244, 270, 284, 403, 451, 477, 797, 924, 948
HgaI GACGC 1 cut(s) 103
HhaI GCGC 5 cut(s) 79, 761, 763, 859, 941
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 2 cut(s) 109, 1030
Hin6I GCGC 5 cut(s) 77, 759, 761, 857, 939
HinP1I GCGC 5 cut(s) 77, 759, 761, 857, 939
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 4 cut(s) 266, 437, 447, 886
HpaII CCGG 9 cut(s) 244, 270, 284, 403, 451, 477, 797, 924, 948
HphI GGTGA 5 cut(s) 295, 307, 334, 811, 1043
Hpy166II GTNNAC 5 cut(s) 187, 232, 303, 802, 962
Hpy188I TCNGA 5 cut(s) 262, 373, 721, 885, 891
Hpy8I GTNNAC 5 cut(s) 187, 232, 303, 802, 962
Hpy99I CGWCG 1 cut(s) 637
HpyAV CCTTC 5 cut(s) 34, 42, 466, 690, 861
HpyCH4III ACNGT 2 cut(s) 307, 581
HpyCH4IV ACGT 2 cut(s) 81, 183
HpyCH4V TGCA 2 cut(s) 962, 1061
HpyF3I CTNAG 2 cut(s) 58, 388
HpySE526I ACGT 2 cut(s) 81, 183
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 2 cut(s) 109, 1030
HspAI GCGC 5 cut(s) 77, 759, 761, 857, 939
KflI GGGWCCC 1 cut(s) 406
Kpn2I TCCGGA 1 cut(s) 450
KroI GCCGGC 1 cut(s) 243
KroNI GCCGGC 1 cut(s) 245
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 5 cut(s) 142, 156, 495, 721, 880
LmnI GCTCC 3 cut(s) 278, 670, 716
LweI GCATC 1 cut(s) 181
MaeI CTAG 3 cut(s) 567, 669, 816
MaeII ACGT 2 cut(s) 81, 183
MaeIII GTNAC 1 cut(s) 734
MalI GATC 5 cut(s) 144, 158, 497, 723, 882
MbiI CCGCTC 1 cut(s) 298
MboI GATC 5 cut(s) 142, 156, 495, 721, 880
MboII GAAGA 7 cut(s) 205, 214, 346, 778, 919, 1004, 1007
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 3 cut(s) 589, 923, 964
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 14, 874, 1051
MluNI TGGCCA 1 cut(s) 251
MlyI GAGTC 2 cut(s) 260, 431
MmeI TCCRAC 1 cut(s) 396
Mox20I TGGCCA 1 cut(s) 251
MroI TCCGGA 1 cut(s) 450
MroNI GCCGGC 1 cut(s) 243
MscI TGGCCA 1 cut(s) 251
MseI TTAA 1 cut(s) 983
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 9 cut(s) 244, 270, 284, 403, 451, 477, 797, 924, 948
MspR9I CCNGG 4 cut(s) 284, 404, 488, 843
MvaI CCWGG 2 cut(s) 488, 843
MvnI CGCG 5 cut(s) 77, 218, 501, 612, 761
NaeI GCCGGC 1 cut(s) 245
NciI CCSGG 2 cut(s) 284, 404
NdeII GATC 5 cut(s) 142, 156, 495, 721, 880
NgoMIV GCCGGC 1 cut(s) 243
NlaIII CATG 2 cut(s) 109, 1030
NlaIV GGNNCC 8 cut(s) 234, 274, 397, 407, 408, 586, 666, 922
NruI TCGCGA 1 cut(s) 612
PaeR7I CTCGAG 1 cut(s) 730
PasI CCCWGGG 1 cut(s) 487
PauI GCGCGC 1 cut(s) 759
PdiI GCCGGC 1 cut(s) 245
PfeI GAWTC 2 cut(s) 447, 886
PinAI ACCGGT 1 cut(s) 796
PkrI GCNGC 2 cut(s) 129, 534
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 2 cut(s) 260, 431
PpsI GAGTC 2 cut(s) 260, 431
PpuMI RGGWCCY 2 cut(s) 406, 418
Psp5II RGGWCCY 2 cut(s) 406, 418
Psp6I CCWGG 2 cut(s) 486, 841
PspGI CCWGG 2 cut(s) 486, 841
PspN4I GGNNCC 8 cut(s) 234, 274, 397, 407, 408, 586, 666, 922
PspPI GGNCC 7 cut(s) 232, 406, 418, 453, 812, 839, 848
PspPPI RGGWCCY 2 cut(s) 406, 418
PspXI VCTCGAGB 1 cut(s) 730
PsuI RGATCY 1 cut(s) 495
PteI GCGCGC 1 cut(s) 759
PvuI CGATCG 1 cut(s) 159
RruI TCGCGA 1 cut(s) 612
RsaI GTAC 3 cut(s) 728, 749, 1046
RsaNI GTAC 3 cut(s) 727, 748, 1045
SaqAI TTAA 1 cut(s) 983
SatI GCNGC 2 cut(s) 128, 533
Sau3AI GATC 5 cut(s) 142, 156, 495, 721, 880
Sau96I GGNCC 7 cut(s) 232, 406, 418, 453, 812, 839, 848
ScaI AGTACT 1 cut(s) 728
SchI GAGTC 2 cut(s) 260, 431
ScrFI CCNGG 4 cut(s) 284, 404, 488, 843
SduI GDGCHC 3 cut(s) 589, 923, 964
SfaNI GCATC 1 cut(s) 181
SfiI GGCCNNNNNGGCC 1 cut(s) 847
Sfr274I CTCGAG 1 cut(s) 730
SinI GGWCC 4 cut(s) 232, 406, 418, 453
SlaI CTCGAG 1 cut(s) 730
SmlI CTYRAG 4 cut(s) 176, 206, 413, 730
SmoI CTYRAG 4 cut(s) 176, 206, 413, 730
Sse9I AATT 3 cut(s) 14, 874, 1051
SspMI CTAG 3 cut(s) 567, 669, 816
StyD4I CCNGG 4 cut(s) 282, 402, 486, 841
StyI CCWWGG 1 cut(s) 776
TaaI ACNGT 2 cut(s) 307, 581
TaiI ACGT 2 cut(s) 84, 186
TaqI TCGA 9 cut(s) 141, 147, 348, 440, 517, 731, 789, 872, 1017
TaqII GACCGA 1 cut(s) 807
TasI AATT 3 cut(s) 14, 874, 1051
TatI WGTACW 2 cut(s) 726, 1044
TauI GCSGC 2 cut(s) 130, 535
TfiI GAWTC 2 cut(s) 447, 886
Tru1I TTAA 1 cut(s) 983
Tru9I TTAA 1 cut(s) 983
TscAI CASTG 2 cut(s) 948, 969
TspDTI ATGAA 1 cut(s) 1005
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 2 cut(s) 948, 969
VneI GTGCAC 1 cut(s) 960
VpaK11BI GGWCC 4 cut(s) 232, 406, 418, 453
XapI RAATTY 1 cut(s) 14
XhoI CTCGAG 1 cut(s) 730
XspI CTAG 3 cut(s) 567, 669, 816
ZrmI AGTACT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.