MD01G1147700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001015
Physical Location & Seq
Forward (+)
25673812 .. 25674807
996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1147700.v1.1.491

Sequence Viewer

Length: 939 bp
ATGGCTTCCTCCCGCCCTCCGCCGCAGAAGCCGCTCGATCTCGACCTCACGATCGTCTCCGCCAAGCATCTCAAGAACGTCAACTGGAAGAACGGCGACCTCAAGCCCTACGCCGTCTTCTGGGTAGACCCCGACCGCCGGCTCGCCACCAAGTCCGACGACTCCGGCTCCACCCGCCCGGTCTGGAACGAGCGGTTCACCGTCCCGCTCACCCTCTCCCTCCACGACTCCTTCCTCACCCTCGAAATCTTCCACTCCAAACCCTCCGAAACTTCCAAGCCCTTAGTCGGCACCCTCCGGGTCCCACTCAAGGACCTGCCCGATCAGGACGACTCGACCCGAATCCGGACCTTCCAGCTCGTCCGTCCCTCCGGCCGTCCCCAGGGCAAGATCCGTGTTAAGCTTGCCGTTCGAGAACGACCTTTGCCGCCAGATTACCATATTAGCCCTCCTCCTAGCTACTTTTACGGAGGTGCCCCTATGCCCCCACCGCCTGTTCGCGACTTCAGGTCATACTCGCCGTCGTCGTACAGCTCTACTTTACCAGCTTCCGCTCCTAGTCCATCGGCTTCGCCACCGCCTCCGCCTCCGCCTTACCATTACAGCTCCTATTCCGATCCGTACTCGGGTTACTATCCAGCGTACTACTCCAGTGCGCCACCGCATCCACCAAGGCCGTTCTTCGACCGACCGGTGAACTATGGCGGGCCTAGTGGGCCTTCAGCGCCATTGGACTACTCAAATTATGACCAGAATCAGAAGCCCAAAAGTGGGAAGATGGGATTGGGCACCGGATTGGCTGTGGGCGCAGTGGCCGGAGGTCTTGGTGCAGTGGCATTGGATGAGGGGTTGAGGTACGAGGAAGATAAGGTTGCACAGAGGGTGGAGAATGACCTGCGCGAGCGTGACGAGTACAGCAATTATCATGCCGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
C2

Protein Analysis

313

Amino Acids

34.54

Weight (kDa)

8.77

Isoelectric Point (pI)

60.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 14 - 112 8.9e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 324, 903
AccB1I GGYRCC 3 cut(s) 290, 473, 788
AccBSI CCGCTC 4 cut(s) 34, 193, 208, 554
AccI GTMKAC 1 cut(s) 126
AccII CGCG 2 cut(s) 501, 900
AccIII TCCGGA 1 cut(s) 345
AclWI GGATC 2 cut(s) 385, 611
AcoI YGGCCR 2 cut(s) 373, 813
AcuI CTGAAG 2 cut(s) 490, 705
AfaI GTAC 5 cut(s) 530, 623, 644, 857, 914
AfiI CCNNNNNNNGG 9 cut(s) 120, 138, 287, 310, 345, 382, 626, 770, 771
AgeI ACCGGT 1 cut(s) 691
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 1 cut(s) 381
AjuI GAANNNNNNNTTGG 2 cut(s) 767, 799
AluBI AGCT 6 cut(s) 358, 403, 459, 534, 548, 606
AluI AGCT 6 cut(s) 358, 403, 459, 534, 548, 606
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 385, 611
Ama87I CYCGRG 1 cut(s) 625
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 5 cut(s) 373, 674, 707, 716, 813
AsiGI ACCGGT 1 cut(s) 691
AspLEI GCGC 4 cut(s) 658, 727, 809, 900
AspS9I GGNCC 5 cut(s) 301, 313, 348, 707, 716
AsuC2I CCSGG 2 cut(s) 179, 299
AsuHPI GGTGA 4 cut(s) 190, 202, 229, 706
AvaI CYCGRG 1 cut(s) 625
AvaII GGWCC 3 cut(s) 301, 313, 348
BaeGI GKGCMC 2 cut(s) 478, 791
BanI GGYRCC 3 cut(s) 290, 473, 788
BbsI GAAGAC 1 cut(s) 109
BccI CCATC 2 cut(s) 571, 772
BceAI ACGGC 6 cut(s) 98, 109, 360, 392, 505, 661
BciT130I CCWGG 1 cut(s) 383
BcnI CCSGG 2 cut(s) 179, 299
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 3 cut(s) 456, 558, 711
BfoI RGCGCY 1 cut(s) 728
BfuAI ACCTGC 2 cut(s) 324, 903
BglI GCCNNNNNGGC 1 cut(s) 715
BisI GCNGC 3 cut(s) 23, 32, 428
BlsI GCNGC 3 cut(s) 24, 33, 429
Bme1390I CCNGG 3 cut(s) 179, 299, 383
Bme18I GGWCC 3 cut(s) 301, 313, 348
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 625
BmgT120I GGNCC 5 cut(s) 301, 313, 348, 707, 716
BmiI GGNNCC 6 cut(s) 169, 292, 302, 303, 475, 790
BmrFI CCNGG 3 cut(s) 179, 299, 383
BmsI GCATC 2 cut(s) 76, 673
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 634
BpuEI CTTGAG 3 cut(s) 56, 86, 293
BpuMI CCSGG 2 cut(s) 179, 299
BsaJI CCNNGG 3 cut(s) 381, 382, 671
BsaWI WCCGGW 3 cut(s) 345, 691, 791
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 9 cut(s) 120, 138, 287, 310, 345, 382, 626, 770, 771
Bse118I RCCGGY 2 cut(s) 138, 691
Bse1I ACTGG 2 cut(s) 89, 651
BseAI TCCGGA 1 cut(s) 345
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 3 cut(s) 381, 382, 671
BseGI GGATG 2 cut(s) 664, 847
BseLI CCNNNNNNNGG 9 cut(s) 120, 138, 287, 310, 345, 382, 626, 770, 771
BseNI ACTGG 2 cut(s) 89, 651
BseRI GAGGAG 1 cut(s) 441
BseSI GKGCMC 2 cut(s) 478, 791
BseX3I CGGCCG 1 cut(s) 373
BsgI GTGCAG 1 cut(s) 849
Bsh1236I CGCG 2 cut(s) 501, 900
Bsh1285I CGRYCG 5 cut(s) 54, 136, 376, 688, 692
BshFI GGCC 5 cut(s) 375, 676, 709, 718, 815
BshNI GGYRCC 3 cut(s) 290, 473, 788
BshTI ACCGGT 1 cut(s) 691
BsiEI CGRYCG 5 cut(s) 54, 136, 376, 688, 692
BsiHKCI CYCGRG 1 cut(s) 625
BsiSI CCGG 9 cut(s) 139, 165, 179, 298, 346, 372, 692, 792, 816
BslFI GGGAC 4 cut(s) 188, 287, 351, 363
BslI CCNNNNNNNGG 9 cut(s) 120, 138, 287, 310, 345, 382, 626, 770, 771
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 4 cut(s) 188, 287, 351, 363
BsnI GGCC 5 cut(s) 375, 676, 709, 718, 815
BsoBI CYCGRG 1 cut(s) 625
Bsp1286I GDGCHC 2 cut(s) 478, 791
Bsp13I TCCGGA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 37, 51, 322, 390, 616
Bsp68I TCGCGA 1 cut(s) 501
BspANI GGCC 5 cut(s) 375, 676, 709, 718, 815
BspEI TCCGGA 1 cut(s) 345
BspFNI CGCG 2 cut(s) 501, 900
BspLI GGNNCC 6 cut(s) 169, 292, 302, 303, 475, 790
BspMI ACCTGC 2 cut(s) 324, 903
BspPI GGATC 2 cut(s) 385, 611
BspT107I GGYRCC 3 cut(s) 290, 473, 788
BsrBI CCGCTC 4 cut(s) 34, 193, 208, 554
BsrFI RCCGGY 2 cut(s) 138, 691
BsrI ACTGG 2 cut(s) 89, 651
BssAI RCCGGY 2 cut(s) 138, 691
BssECI CCNNGG 3 cut(s) 381, 382, 671
BssMI GATC 5 cut(s) 37, 51, 322, 390, 616
BssT1I CCWWGG 1 cut(s) 671
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 1 cut(s) 202
BstC8I GCNNGC 5 cut(s) 140, 144, 405, 707, 902
BstDEI CTNAG 1 cut(s) 283
BstF5I GGATG 2 cut(s) 664, 847
BstFNI CGCG 2 cut(s) 501, 900
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 4 cut(s) 658, 727, 809, 900
BstKTI GATC 5 cut(s) 40, 54, 325, 393, 619
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 37, 51, 322, 390, 616
BstMCI CGRYCG 5 cut(s) 54, 136, 376, 688, 692
BstMWI GCNNNNNNNGC 7 cut(s) 28, 31, 174, 490, 715, 724, 806
BstNI CCWGG 1 cut(s) 383
BstSCI CCNGG 3 cut(s) 177, 297, 381
BstSLI GKGCMC 2 cut(s) 478, 791
BstUI CGCG 2 cut(s) 501, 900
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BstZI CGGCCG 1 cut(s) 373
BsuRI GGCC 5 cut(s) 375, 676, 709, 718, 815
BtsCI GGATG 2 cut(s) 664, 847
BtsI GCAGTG 2 cut(s) 816, 837
BtsIMutI CAGTG 3 cut(s) 658, 816, 837
BtuMI TCGCGA 1 cut(s) 501
BveI ACCTGC 2 cut(s) 324, 903
Cac8I GCNNGC 5 cut(s) 140, 144, 405, 707, 902
CfoI GCGC 4 cut(s) 658, 727, 809, 900
Cfr10I RCCGGY 2 cut(s) 138, 691
Cfr13I GGNCC 5 cut(s) 301, 313, 348, 707, 716
Csp6I GTAC 5 cut(s) 529, 622, 643, 856, 913
CspAI ACCGGT 1 cut(s) 691
CviAII CATG 1 cut(s) 926
CviQI GTAC 5 cut(s) 529, 622, 643, 856, 913
DdeI CTNAG 1 cut(s) 283
DpnI GATC 5 cut(s) 39, 53, 324, 392, 618
DpnII GATC 5 cut(s) 37, 51, 322, 390, 616
DriI GACNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 373, 813
EagI CGGCCG 1 cut(s) 373
Eam1105I GACNNNNNGTC 1 cut(s) 508
EciI GGCGGA 4 cut(s) 9, 49, 573, 579
EclXI CGGCCG 1 cut(s) 373
Eco130I CCWWGG 1 cut(s) 671
Eco47I GGWCC 3 cut(s) 301, 313, 348
Eco52I CGGCCG 1 cut(s) 373
Eco57I CTGAAG 2 cut(s) 490, 705
Eco88I CYCGRG 1 cut(s) 625
EcoO109I RGGNCCY 2 cut(s) 301, 313
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 671
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 1 cut(s) 929
FaiI YATR 6 cut(s) 441, 482, 514, 702, 747, 927
FaqI GGGAC 4 cut(s) 188, 287, 351, 363
FatI CATG 1 cut(s) 925
FauI CCCGC 4 cut(s) 20, 182, 213, 698
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 23, 32, 428
FokI GGATG 2 cut(s) 651, 854
Fsp4HI GCNGC 3 cut(s) 23, 32, 428
FspBI CTAG 3 cut(s) 456, 558, 711
GlaI GCGC 4 cut(s) 657, 726, 808, 899
GluI GCNGC 3 cut(s) 23, 32, 428
GsuI CTGGAG 1 cut(s) 634
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 5 cut(s) 375, 676, 709, 718, 815
HapII CCGG 9 cut(s) 139, 165, 179, 298, 346, 372, 692, 792, 816
HhaI GCGC 4 cut(s) 658, 727, 809, 900
Hin1II CATG 1 cut(s) 929
Hin6I GCGC 4 cut(s) 656, 725, 807, 898
HinP1I GCGC 4 cut(s) 656, 725, 807, 898
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 5 cut(s) 161, 227, 332, 342, 754
HpaII CCGG 9 cut(s) 139, 165, 179, 298, 346, 372, 692, 792, 816
HphI GGTGA 4 cut(s) 190, 202, 229, 706
Hpy166II GTNNAC 4 cut(s) 82, 127, 198, 697
Hpy188I TCNGA 4 cut(s) 157, 268, 616, 759
Hpy188III TCNNGA 8 cut(s) 41, 49, 73, 184, 326, 346, 413, 500
Hpy8I GTNNAC 4 cut(s) 82, 127, 198, 697
Hpy99I CGWCG 3 cut(s) 161, 526, 529
HpyAV CCTTC 3 cut(s) 241, 361, 729
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 2 cut(s) 830, 875
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 31, 174, 490, 715, 724, 806
HpyF3I CTNAG 1 cut(s) 283
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 1 cut(s) 929
HspAI GCGC 4 cut(s) 656, 725, 807, 898
KflI GGGWCCC 1 cut(s) 301
Kpn2I TCCGGA 1 cut(s) 345
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Kzo9I GATC 5 cut(s) 37, 51, 322, 390, 616
LmnI GCTCC 3 cut(s) 173, 559, 611
LweI GCATC 2 cut(s) 76, 673
MaeI CTAG 3 cut(s) 456, 558, 711
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 629, 905
MalI GATC 5 cut(s) 39, 53, 324, 392, 618
MbiI CCGCTC 4 cut(s) 34, 193, 208, 554
MboI GATC 5 cut(s) 37, 51, 322, 390, 616
MboII GAAGA 6 cut(s) 100, 109, 241, 673, 787, 875
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 2 cut(s) 478, 791
MluCI AATT 2 cut(s) 742, 919
MlyI GAGTC 3 cut(s) 155, 221, 326
MmeI TCCRAC 1 cut(s) 180
MroI TCCGGA 1 cut(s) 345
MroNI GCCGGC 1 cut(s) 138
MseI TTAA 1 cut(s) 399
MspI CCGG 9 cut(s) 139, 165, 179, 298, 346, 372, 692, 792, 816
MspR9I CCNGG 3 cut(s) 179, 299, 383
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 2 cut(s) 501, 900
MwoI GCNNNNNNNGC 7 cut(s) 28, 31, 174, 490, 715, 724, 806
NaeI GCCGGC 1 cut(s) 140
NciI CCSGG 2 cut(s) 179, 299
NdeII GATC 5 cut(s) 37, 51, 322, 390, 616
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 1 cut(s) 929
NlaIV GGNNCC 6 cut(s) 169, 292, 302, 303, 475, 790
NmuCI GTSAC 1 cut(s) 905
NruI TCGCGA 1 cut(s) 501
PasI CCCWGGG 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 524
PdiI GCCGGC 1 cut(s) 140
PfeI GAWTC 2 cut(s) 342, 754
PinAI ACCGGT 1 cut(s) 691
PkrI GCNGC 3 cut(s) 24, 33, 429
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 3 cut(s) 155, 221, 326
PpsI GAGTC 3 cut(s) 155, 221, 326
PpuMI RGGWCCY 2 cut(s) 301, 313
Psp5II RGGWCCY 2 cut(s) 301, 313
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 6 cut(s) 169, 292, 302, 303, 475, 790
PspPI GGNCC 5 cut(s) 301, 313, 348, 707, 716
PspPPI RGGWCCY 2 cut(s) 301, 313
PsuI RGATCY 1 cut(s) 390
PvuI CGATCG 1 cut(s) 54
RruI TCGCGA 1 cut(s) 501
RsaI GTAC 5 cut(s) 530, 623, 644, 857, 914
RsaNI GTAC 5 cut(s) 529, 622, 643, 856, 913
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 3 cut(s) 23, 32, 428
Sau3AI GATC 5 cut(s) 37, 51, 322, 390, 616
Sau96I GGNCC 5 cut(s) 301, 313, 348, 707, 716
SchI GAGTC 3 cut(s) 155, 221, 326
ScrFI CCNGG 3 cut(s) 179, 299, 383
SduI GDGCHC 2 cut(s) 478, 791
SfaNI GCATC 2 cut(s) 76, 673
SfiI GGCCNNNNNGGCC 1 cut(s) 715
SinI GGWCC 3 cut(s) 301, 313, 348
SmlI CTYRAG 3 cut(s) 71, 101, 308
SmoI CTYRAG 3 cut(s) 71, 101, 308
Sse9I AATT 2 cut(s) 742, 919
SspMI CTAG 3 cut(s) 456, 558, 711
StyD4I CCNGG 3 cut(s) 177, 297, 381
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 81
TaqI TCGA 6 cut(s) 36, 42, 243, 335, 412, 684
TaqII GACCGA 1 cut(s) 702
TasI AATT 2 cut(s) 742, 919
TatI WGTACW 1 cut(s) 912
TauI GCSGC 3 cut(s) 25, 34, 430
TfiI GAWTC 2 cut(s) 342, 754
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 3 cut(s) 658, 816, 837
TseFI GTSAC 1 cut(s) 905
Tsp45I GTSAC 1 cut(s) 905
TspGWI ACGGA 4 cut(s) 353, 383, 483, 609
TspRI CASTG 3 cut(s) 658, 816, 837
VpaK11BI GGWCC 3 cut(s) 301, 313, 348
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 3 cut(s) 456, 558, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.