Transferase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 59, 186, 206, 243 |
| AclWI | GGATC | 1 cut(s) | 354 |
| AfaI | GTAC | 4 cut(s) | 36, 133, 156, 164 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 470 |
| AgsI | TTSAA | 1 cut(s) | 374 |
| AjnI | CCWGG | 1 cut(s) | 145 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 303, 335 |
| AlwI | GGATC | 1 cut(s) | 354 |
| AoxI | GGCC | 1 cut(s) | 143 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 453, 485 |
| AsuHPI | GGTGA | 2 cut(s) | 175, 412 |
| BbsI | GAAGAC | 1 cut(s) | 292 |
| BciT130I | CCWGG | 1 cut(s) | 147 |
| BfaI | CTAG | 1 cut(s) | 83 |
| Bme1390I | CCNGG | 1 cut(s) | 147 |
| BmiI | GGNNCC | 1 cut(s) | 352 |
| BmrFI | CCNGG | 1 cut(s) | 147 |
| BpiI | GAAGAC | 1 cut(s) | 292 |
| BpuEI | CTTGAG | 1 cut(s) | 269 |
| BsaAI | YACGTR | 1 cut(s) | 503 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 303, 333, 335, 365 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 470 |
| Bse1I | ACTGG | 2 cut(s) | 170, 272 |
| BseBI | CCWGG | 1 cut(s) | 147 |
| BseGI | GGATG | 2 cut(s) | 55, 165 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 470 |
| BseNI | ACTGG | 2 cut(s) | 170, 272 |
| BseRI | GAGGAG | 3 cut(s) | 181, 311, 314 |
| BshFI | GGCC | 1 cut(s) | 145 |
| BsiSI | CCGG | 1 cut(s) | 348 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 470 |
| BsnI | GGCC | 1 cut(s) | 145 |
| Bsp143I | GATC | 3 cut(s) | 79, 220, 359 |
| BspACI | CCGC | 4 cut(s) | 59, 186, 206, 243 |
| BspANI | GGCC | 1 cut(s) | 145 |
| BspLI | GGNNCC | 1 cut(s) | 352 |
| BspPI | GGATC | 1 cut(s) | 354 |
| BsrI | ACTGG | 2 cut(s) | 170, 272 |
| BssMI | GATC | 3 cut(s) | 79, 220, 359 |
| Bst2UI | CCWGG | 1 cut(s) | 147 |
| Bst4CI | ACNGT | 3 cut(s) | 39, 76, 136 |
| BstBAI | YACGTR | 1 cut(s) | 503 |
| BstF5I | GGATG | 2 cut(s) | 55, 165 |
| BstKTI | GATC | 3 cut(s) | 82, 223, 362 |
| BstMBI | GATC | 3 cut(s) | 79, 220, 359 |
| BstNI | CCWGG | 1 cut(s) | 147 |
| BstNSI | RCATGY | 1 cut(s) | 59 |
| BstSCI | CCNGG | 1 cut(s) | 145 |
| BstV2I | GAAGAC | 1 cut(s) | 292 |
| BsuRI | GGCC | 1 cut(s) | 145 |
| BtsCI | GGATG | 2 cut(s) | 55, 165 |
| Csp6I | GTAC | 4 cut(s) | 35, 132, 155, 163 |
| CviAII | CATG | 1 cut(s) | 56 |
| CviJI | RGCY | 5 cut(s) | 145, 214, 289, 351, 474 |
| CviKI_1 | RGCY | 5 cut(s) | 145, 214, 289, 351, 474 |
| CviQI | GTAC | 4 cut(s) | 35, 132, 155, 163 |
| DpnI | GATC | 3 cut(s) | 81, 222, 361 |
| DpnII | GATC | 3 cut(s) | 79, 220, 359 |
| EciI | GGCGGA | 1 cut(s) | 232 |
| Eco147I | AGGCCT | 1 cut(s) | 145 |
| EcoRII | CCWGG | 1 cut(s) | 145 |
| FaeI | CATG | 1 cut(s) | 59 |
| FaiI | YATR | 3 cut(s) | 9, 57, 467 |
| FatI | CATG | 1 cut(s) | 55 |
| FokI | GGATG | 2 cut(s) | 62, 172 |
| FspBI | CTAG | 1 cut(s) | 83 |
| HaeIII | GGCC | 1 cut(s) | 145 |
| HapII | CCGG | 1 cut(s) | 348 |
| Hin1II | CATG | 1 cut(s) | 59 |
| HincII | GTYRAC | 1 cut(s) | 265 |
| HindII | GTYRAC | 1 cut(s) | 265 |
| HinfI | GANTC | 2 cut(s) | 344, 410 |
| HpaII | CCGG | 1 cut(s) | 348 |
| HphI | GGTGA | 2 cut(s) | 175, 412 |
| Hpy166II | GTNNAC | 2 cut(s) | 155, 265 |
| Hpy188I | TCNGA | 2 cut(s) | 340, 451 |
| Hpy188III | TCNNGA | 3 cut(s) | 218, 224, 367 |
| Hpy8I | GTNNAC | 2 cut(s) | 155, 265 |
| HpyAV | CCTTC | 3 cut(s) | 5, 406, 424 |
| HpyCH4III | ACNGT | 3 cut(s) | 39, 76, 136 |
| HpyCH4IV | ACGT | 2 cut(s) | 261, 502 |
| HpyCH4V | TGCA | 1 cut(s) | 120 |
| HpySE526I | ACGT | 2 cut(s) | 261, 502 |
| Hsp92II | CATG | 1 cut(s) | 59 |
| Kzo9I | GATC | 3 cut(s) | 79, 220, 359 |
| LmnI | GCTCC | 1 cut(s) | 356 |
| LpnPI | CCDG | 6 cut(s) | 132, 151, 159, 253, 289, 361 |
| MaeI | CTAG | 1 cut(s) | 83 |
| MaeII | ACGT | 2 cut(s) | 261, 502 |
| MaeIII | GTNAC | 2 cut(s) | 70, 503 |
| MalI | GATC | 3 cut(s) | 81, 222, 361 |
| MboI | GATC | 3 cut(s) | 79, 220, 359 |
| MboII | GAAGA | 3 cut(s) | 283, 292, 424 |
| MluCI | AATT | 1 cut(s) | 121 |
| MlyI | GAGTC | 2 cut(s) | 338, 404 |
| MmeI | TCCRAC | 1 cut(s) | 474 |
| MseI | TTAA | 2 cut(s) | 41, 170 |
| MspI | CCGG | 1 cut(s) | 348 |
| MspR9I | CCNGG | 1 cut(s) | 147 |
| MvaI | CCWGG | 1 cut(s) | 147 |
| NdeII | GATC | 3 cut(s) | 79, 220, 359 |
| NlaIII | CATG | 1 cut(s) | 59 |
| NlaIV | GGNNCC | 1 cut(s) | 352 |
| NmuCI | GTSAC | 1 cut(s) | 503 |
| NspI | RCATGY | 1 cut(s) | 59 |
| PceI | AGGCCT | 1 cut(s) | 145 |
| PleI | GAGTC | 2 cut(s) | 338, 404 |
| PpsI | GAGTC | 2 cut(s) | 338, 404 |
| Ppu21I | YACGTR | 1 cut(s) | 503 |
| Psp6I | CCWGG | 1 cut(s) | 145 |
| PspGI | CCWGG | 1 cut(s) | 145 |
| PspN4I | GGNNCC | 1 cut(s) | 352 |
| RsaI | GTAC | 4 cut(s) | 36, 133, 156, 164 |
| RsaNI | GTAC | 4 cut(s) | 35, 132, 155, 163 |
| SaqAI | TTAA | 2 cut(s) | 41, 170 |
| Sau3AI | GATC | 3 cut(s) | 79, 220, 359 |
| SchI | GAGTC | 2 cut(s) | 338, 404 |
| ScrFI | CCNGG | 1 cut(s) | 147 |
| SetI | ASST | 8 cut(s) | 231, 255, 264, 285, 321, 486, 501, 505 |
| SmlI | CTYRAG | 1 cut(s) | 284 |
| SmoI | CTYRAG | 1 cut(s) | 284 |
| Sse9I | AATT | 1 cut(s) | 121 |
| SseBI | AGGCCT | 1 cut(s) | 145 |
| SsiI | CCGC | 4 cut(s) | 59, 186, 206, 243 |
| SspMI | CTAG | 1 cut(s) | 83 |
| StuI | AGGCCT | 1 cut(s) | 145 |
| StyD4I | CCNGG | 1 cut(s) | 145 |
| TaaI | ACNGT | 3 cut(s) | 39, 76, 136 |
| TaiI | ACGT | 2 cut(s) | 264, 505 |
| TaqI | TCGA | 4 cut(s) | 78, 225, 309, 426 |
| TasI | AATT | 1 cut(s) | 121 |
| TatI | WGTACW | 1 cut(s) | 162 |
| Tru1I | TTAA | 2 cut(s) | 41, 170 |
| Tru9I | TTAA | 2 cut(s) | 41, 170 |
| TseFI | GTSAC | 1 cut(s) | 503 |
| Tsp45I | GTSAC | 1 cut(s) | 503 |
| TspDTI | ATGAA | 2 cut(s) | 24, 66 |
| TspGWI | ACGGA | 2 cut(s) | 172, 352 |
| XceI | RCATGY | 1 cut(s) | 59 |
| XspI | CTAG | 1 cut(s) | 83 |