Rorug01G0350600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001015
Physical Location & Seq
Forward (+)
46801295 .. 46801759
465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0350600.1

Sequence Viewer

Length: 465 bp
ATGCGTGCACGTTCTGTGCAGTCACAAGCTAGTCAGGGACATTGCAGGCCCCTCAAACCTTGGACTCCCCCTCCTATTGGATGGTTGAAAATCAACCTTGATGGTGGCTTTCAGAAGAATACCAACAAGGGTGGTATTGGTTTCATTGTAAGGGATCATTTAGGAACTGTTGTGGGAGGTACGCCAGATGTGGTTGAGGCCATGGCAGGCCGTGCAGCAGTTCAATATGCAGTGGAGTACGGGTTGGCTCCTGTGAGTTTTGAAACCGACTGCCTTAAACTGGTTAACTCATTACATTCTTATGGTAATGATCTCTCTGGTTTTGGCACTATAGTTGGGGACATTGTGTTCCTCAAAGAATCTATTCCTTGTTCAACTTTTGTTCATTGTTATAGGGAGGCTAATTCAGTAGCCCATAGGCTAGCTAGTTTAGCTCTCAACTCAAATTTAGATCTTGTATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

154

Amino Acids

16.61

Weight (kDa)

7.72

Isoelectric Point (pI)

18.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 32 - 144 1.1e-16 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 1 cut(s) 445
AfaI GTAC 2 cut(s) 181, 239
AfiI CCNNNNNNNGG 2 cut(s) 77, 280
AgsI TTSAA 4 cut(s) 88, 224, 263, 375
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 3 cut(s) 29, 425, 434
AluI AGCT 3 cut(s) 29, 425, 434
Alw21I GWGCWC 1 cut(s) 10
Alw44I GTGCAC 1 cut(s) 6
AlwI GGATC 1 cut(s) 162
AoxI GGCC 3 cut(s) 47, 198, 208
ApaLI GTGCAC 1 cut(s) 6
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 445
Asp700I GAANNNNTTC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 48
AsuNHI GCTAGC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 75, 95
BceAI ACGGC 1 cut(s) 195
BfaI CTAG 3 cut(s) 30, 422, 426
BfmI CTRYAG 1 cut(s) 330
BglII AGATCT 1 cut(s) 451
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 50, 249
BmtI GCTAGC 1 cut(s) 425
BsaJI CCNNGG 2 cut(s) 59, 201
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 280
Bse1I ACTGG 1 cut(s) 285
Bse3DI GCAATG 1 cut(s) 40
BseDI CCNNGG 2 cut(s) 59, 201
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 2 cut(s) 77, 280
BseMI GCAATG 1 cut(s) 40
BseNI ACTGG 1 cut(s) 285
BseSI GKGCMC 1 cut(s) 10
BseXI GCAGC 1 cut(s) 227
BsgI GTGCAG 2 cut(s) 38, 234
BshFI GGCC 3 cut(s) 49, 200, 210
BsiHKAI GWGCWC 1 cut(s) 10
BslFI GGGAC 2 cut(s) 51, 353
BslI CCNNNNNNNGG 2 cut(s) 77, 280
BsmFI GGGAC 2 cut(s) 51, 353
BsnI GGCC 3 cut(s) 49, 200, 210
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 3 cut(s) 154, 310, 451
Bsp19I CCATGG 1 cut(s) 201
BspANI GGCC 3 cut(s) 49, 200, 210
BspLI GGNNCC 2 cut(s) 50, 249
BspOI GCTAGC 1 cut(s) 425
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 1 cut(s) 285
BssECI CCNNGG 2 cut(s) 59, 201
BssMI GATC 3 cut(s) 154, 310, 451
BssT1I CCWWGG 2 cut(s) 59, 201
Bst4CI ACNGT 1 cut(s) 169
BstAPI GCANNNNNTGC 1 cut(s) 212
BstC8I GCNNGC 4 cut(s) 6, 47, 208, 423
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 86
BstKTI GATC 3 cut(s) 157, 313, 454
BstMBI GATC 3 cut(s) 154, 310, 451
BstMWI GCNNNNNNNGC 2 cut(s) 212, 431
BstSFI CTRYAG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 10
BstV1I GCAGC 1 cut(s) 227
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BsuRI GGCC 3 cut(s) 49, 200, 210
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 4 cut(s) 6, 47, 208, 423
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 2 cut(s) 180, 238
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 1 cut(s) 202
CviQI GTAC 2 cut(s) 180, 238
DpnI GATC 3 cut(s) 156, 312, 453
DpnII GATC 3 cut(s) 154, 310, 451
Eco130I CCWWGG 2 cut(s) 59, 201
EcoO109I RGGNCCY 1 cut(s) 48
EcoT14I CCWWGG 2 cut(s) 59, 201
ErhI CCWWGG 2 cut(s) 59, 201
FaeI CATG 1 cut(s) 205
FaiI YATR 7 cut(s) 203, 228, 303, 332, 393, 417, 460
FaqI GGGAC 2 cut(s) 51, 353
FatI CATG 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 3 cut(s) 30, 422, 426
GluI GCNGC 1 cut(s) 216
HaeIII GGCC 3 cut(s) 49, 200, 210
Hin1II CATG 1 cut(s) 205
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 2 cut(s) 64, 359
HpaI GTTAAC 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 8, 286
Hpy188I TCNGA 1 cut(s) 114
Hpy8I GTNNAC 2 cut(s) 8, 286
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 5 cut(s) 8, 19, 45, 215, 230
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 431
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 1 cut(s) 205
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 3 cut(s) 154, 310, 451
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 7 cut(s) 20, 31, 192, 198, 264, 266, 303
Lsp1109I GCAGC 1 cut(s) 227
MaeI CTAG 3 cut(s) 30, 422, 426
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 21
MalI GATC 3 cut(s) 156, 312, 453
MboI GATC 3 cut(s) 154, 310, 451
MboII GAAGA 1 cut(s) 127
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 2 cut(s) 403, 445
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 6 cut(s) 62, 81, 170, 190, 362, 391
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 2 cut(s) 276, 285
MslI CAYNNNNRTG 1 cut(s) 300
MwoI GCNNNNNNNGC 2 cut(s) 212, 431
NcoI CCATGG 1 cut(s) 201
NdeII GATC 3 cut(s) 154, 310, 451
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 1 cut(s) 205
NlaIV GGNNCC 2 cut(s) 50, 249
NmuCI GTSAC 1 cut(s) 21
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 359
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PspN4I GGNNCC 2 cut(s) 50, 249
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 451
RsaI GTAC 2 cut(s) 181, 239
RsaNI GTAC 2 cut(s) 180, 238
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 2 cut(s) 276, 285
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 154, 310, 451
Sau96I GGNCC 1 cut(s) 48
SchI GAGTC 1 cut(s) 58
SduI GDGCHC 1 cut(s) 10
SetI ASST 7 cut(s) 13, 31, 61, 99, 181, 427, 436
SfcI CTRYAG 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 2 cut(s) 403, 445
SspMI CTAG 3 cut(s) 30, 422, 426
StyI CCWWGG 2 cut(s) 59, 201
TaaI ACNGT 1 cut(s) 169
TaiI ACGT 1 cut(s) 13
TasI AATT 2 cut(s) 403, 445
TfiI GAWTC 1 cut(s) 359
Tru1I TTAA 2 cut(s) 276, 285
Tru9I TTAA 2 cut(s) 276, 285
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 133, 374
TspRI CASTG 1 cut(s) 237
VneI GTGCAC 1 cut(s) 6
XapI RAATTY 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 3 cut(s) 30, 422, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.