pycom01g16650

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001015
Physical Location & Seq
Forward (+)
16162409 .. 16163349
941 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16650.2

Sequence Viewer

Length: 900 bp
ATGGCTTCATCCCGCCCTCCGCCGCAGAAGCCTCTCGATCTCGACCTCACGATCGTCTCCGCCAAGCATCTCAAGAACGTCAACTGGAAGAACGGCGACCTCAAGCCCTACGCCGTCTTCTGGGTAGACCCCGACCGCCGGCTCGCCACCAAGTCCGACGACTCCGGCTCCACCCGCCCGGTCTGGAACGAGCGATTCACCGTCCCGCTCACCCTGTCCCTCCACGACTCCTTCCTCACCCTCGAAATCTTCCACTCCAAACCCTCCGACACTCCCAAGCCCTTAGTCGGCACCCTCAGGGACGACTCGACCCGAATCCGGACCTTCCAGCTCGTCCGTCCTTCCGGCCGTCCCCAGGGCAAGATCCGTGTTAAGCTTGCCGTTCGAGAACGACCTTTGCCGCCAGATTACCATATTAGCCCTCCTCCTAGCTACTTTTACGGAGGTGCCTCTATGCCCCCACCGCCTGTTCGCGACTTCAGGTCATACTCGCCGTCGCCGTACAGCTCTACTTTACCAGCTTCCGCTCCTAGTCCGTCGGCTTCGCCACCGCCTTACCATTACAGCTCCTATTCCGATCCGTACTCGGGTTACTATCCAGCGTACTACTCCAGTGCGCCACCGCCTCCACCAAGGCCGTTCTTCGACCGACCGGTGAACTATGGCGGGCCTAGTGGGCCTTCAGCGCCATTGGACTACTCGAATTATGATCAGAATCAGAAGCCCAAGAGTGGGAAGATGGGATTGGGCACCGGATTGGCTGTGGGCGCAGTGGCCGGAGGTCTTGGTGCAGTGGCATTGGATGAGGGGTTGAGGTACGAAGAAGATAAGGTTGCACAGAGGGTGGAGAATGACCTGCGCGAGCGTGACGATTACAGCAATTATCGTGCAGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
C2

Protein Analysis

300

Amino Acids

33.11

Weight (kDa)

9.11

Isoelectric Point (pI)

58.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 864
AccB1I GGYRCC 3 cut(s) 290, 446, 749
AccBSI CCGCTC 2 cut(s) 208, 527
AccI GTMKAC 1 cut(s) 126
AccII CGCG 2 cut(s) 474, 861
AccIII TCCGGA 1 cut(s) 318
AclWI GGATC 2 cut(s) 358, 572
AcoI YGGCCR 2 cut(s) 346, 774
AcuI CTGAAG 2 cut(s) 463, 666
AfaI GTAC 4 cut(s) 503, 584, 605, 818
AfiI CCNNNNNNNGG 8 cut(s) 120, 138, 287, 318, 355, 587, 731, 732
AgeI ACCGGT 1 cut(s) 652
AhdI GACNNNNNGTC 1 cut(s) 481
AjnI CCWGG 1 cut(s) 354
AjuI GAANNNNNNNTTGG 2 cut(s) 728, 760
AluBI AGCT 6 cut(s) 331, 376, 432, 507, 521, 567
AluI AGCT 6 cut(s) 331, 376, 432, 507, 521, 567
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 358, 572
Ama87I CYCGRG 1 cut(s) 586
Aor13HI TCCGGA 1 cut(s) 318
AoxI GGCC 5 cut(s) 346, 635, 668, 677, 774
AsiGI ACCGGT 1 cut(s) 652
AspLEI GCGC 4 cut(s) 619, 688, 770, 861
AspS9I GGNCC 3 cut(s) 321, 668, 677
AsuC2I CCSGG 1 cut(s) 179
AsuHPI GGTGA 4 cut(s) 190, 202, 229, 667
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 752
BanI GGYRCC 3 cut(s) 290, 446, 749
BbsI GAAGAC 1 cut(s) 109
BccI CCATC 1 cut(s) 733
BceAI ACGGC 7 cut(s) 98, 109, 333, 365, 478, 484, 622
BciT130I CCWGG 1 cut(s) 356
BclI TGATCA 1 cut(s) 709
BcnI CCSGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 3 cut(s) 429, 531, 672
BfoI RGCGCY 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 864
BglI GCCNNNNNGGC 1 cut(s) 676
BisI GCNGC 2 cut(s) 23, 401
BlsI GCNGC 2 cut(s) 24, 402
Bme1390I CCNGG 2 cut(s) 179, 356
Bme18I GGWCC 1 cut(s) 321
BmeRI GACNNNNNGTC 1 cut(s) 481
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 321, 668, 677
BmiI GGNNCC 4 cut(s) 169, 292, 448, 751
BmrFI CCNGG 2 cut(s) 179, 356
BmsI GCATC 1 cut(s) 76
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 595
BpuEI CTTGAG 2 cut(s) 56, 86
BpuMI CCSGG 1 cut(s) 179
BsaBI GATNNNNATC 1 cut(s) 714
BsaJI CCNNGG 3 cut(s) 354, 355, 632
BsaWI WCCGGW 3 cut(s) 318, 652, 752
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 8 cut(s) 120, 138, 287, 318, 355, 587, 731, 732
Bse118I RCCGGY 2 cut(s) 138, 652
Bse1I ACTGG 2 cut(s) 89, 612
Bse21I CCTNAGG 1 cut(s) 296
Bse8I GATNNNNATC 1 cut(s) 714
BseAI TCCGGA 1 cut(s) 318
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 354, 355, 632
BseGI GGATG 2 cut(s) 8, 808
BseJI GATNNNNATC 1 cut(s) 714
BseLI CCNNNNNNNGG 8 cut(s) 120, 138, 287, 318, 355, 587, 731, 732
BseMII CTCAG 1 cut(s) 310
BseNI ACTGG 2 cut(s) 89, 612
BseRI GAGGAG 1 cut(s) 414
BseSI GKGCMC 1 cut(s) 752
BseX3I CGGCCG 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 810
Bsh1236I CGCG 2 cut(s) 474, 861
Bsh1285I CGRYCG 5 cut(s) 54, 136, 349, 649, 653
BshFI GGCC 5 cut(s) 348, 637, 670, 679, 776
BshNI GGYRCC 3 cut(s) 290, 446, 749
BshTI ACCGGT 1 cut(s) 652
BsiEI CGRYCG 5 cut(s) 54, 136, 349, 649, 653
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 8 cut(s) 139, 165, 179, 319, 345, 653, 753, 777
BslFI GGGAC 4 cut(s) 188, 202, 314, 336
BslI CCNNNNNNNGG 8 cut(s) 120, 138, 287, 318, 355, 587, 731, 732
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 4 cut(s) 188, 202, 314, 336
BsnI GGCC 5 cut(s) 348, 637, 670, 679, 776
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 752
Bsp13I TCCGGA 1 cut(s) 318
Bsp143I GATC 5 cut(s) 37, 51, 363, 577, 709
Bsp68I TCGCGA 1 cut(s) 474
BspANI GGCC 5 cut(s) 348, 637, 670, 679, 776
BspCNI CTCAG 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 318
BspFNI CGCG 2 cut(s) 474, 861
BspLI GGNNCC 4 cut(s) 169, 292, 448, 751
BspMI ACCTGC 1 cut(s) 864
BspPI GGATC 2 cut(s) 358, 572
BspT107I GGYRCC 3 cut(s) 290, 446, 749
BsrBI CCGCTC 2 cut(s) 208, 527
BsrFI RCCGGY 2 cut(s) 138, 652
BsrI ACTGG 2 cut(s) 89, 612
BssAI RCCGGY 2 cut(s) 138, 652
BssECI CCNNGG 3 cut(s) 354, 355, 632
BssMI GATC 5 cut(s) 37, 51, 363, 577, 709
BssT1I CCWWGG 1 cut(s) 632
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 1 cut(s) 202
BstC8I GCNNGC 5 cut(s) 140, 144, 378, 668, 863
BstDEI CTNAG 2 cut(s) 283, 296
BstF5I GGATG 2 cut(s) 8, 808
BstFNI CGCG 2 cut(s) 474, 861
BstH2I RGCGCY 1 cut(s) 689
BstHHI GCGC 4 cut(s) 619, 688, 770, 861
BstKTI GATC 5 cut(s) 40, 54, 366, 580, 712
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 37, 51, 363, 577, 709
BstMCI CGRYCG 5 cut(s) 54, 136, 349, 649, 653
BstMWI GCNNNNNNNGC 6 cut(s) 28, 174, 463, 676, 685, 767
BstNI CCWGG 1 cut(s) 356
BstSCI CCNGG 2 cut(s) 177, 354
BstSLI GKGCMC 1 cut(s) 752
BstUI CGCG 2 cut(s) 474, 861
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
BstZI CGGCCG 1 cut(s) 346
Bsu36I CCTNAGG 1 cut(s) 296
BsuRI GGCC 5 cut(s) 348, 637, 670, 679, 776
BtsCI GGATG 2 cut(s) 8, 808
BtsI GCAGTG 2 cut(s) 777, 798
BtsIMutI CAGTG 3 cut(s) 619, 777, 798
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 1 cut(s) 864
Cac8I GCNNGC 5 cut(s) 140, 144, 378, 668, 863
CfoI GCGC 4 cut(s) 619, 688, 770, 861
Cfr10I RCCGGY 2 cut(s) 138, 652
Cfr13I GGNCC 3 cut(s) 321, 668, 677
Csp6I GTAC 4 cut(s) 502, 583, 604, 817
CspAI ACCGGT 1 cut(s) 652
CviQI GTAC 4 cut(s) 502, 583, 604, 817
DdeI CTNAG 2 cut(s) 283, 296
DpnI GATC 5 cut(s) 39, 53, 365, 579, 711
DpnII GATC 5 cut(s) 37, 51, 363, 577, 709
DriI GACNNNNNGTC 1 cut(s) 481
EaeI YGGCCR 2 cut(s) 346, 774
EagI CGGCCG 1 cut(s) 346
Eam1105I GACNNNNNGTC 1 cut(s) 481
EciI GGCGGA 2 cut(s) 9, 49
EclXI CGGCCG 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 632
Eco47I GGWCC 1 cut(s) 321
Eco52I CGGCCG 1 cut(s) 346
Eco57I CTGAAG 2 cut(s) 463, 666
Eco81I CCTNAGG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 632
ErhI CCWWGG 1 cut(s) 632
Esp3I CGTCTC 1 cut(s) 61
FaiI YATR 5 cut(s) 414, 455, 487, 663, 708
FaqI GGGAC 4 cut(s) 188, 202, 314, 336
FauI CCCGC 4 cut(s) 20, 182, 213, 659
FbaI TGATCA 1 cut(s) 709
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 2 cut(s) 23, 401
FokI GGATG 1 cut(s) 815
Fsp4HI GCNGC 2 cut(s) 23, 401
FspBI CTAG 3 cut(s) 429, 531, 672
GlaI GCGC 4 cut(s) 618, 687, 769, 860
GluI GCNGC 2 cut(s) 23, 401
GsuI CTGGAG 1 cut(s) 595
HaeII RGCGCY 1 cut(s) 689
HaeIII GGCC 5 cut(s) 348, 637, 670, 679, 776
HapII CCGG 8 cut(s) 139, 165, 179, 319, 345, 653, 753, 777
HhaI GCGC 4 cut(s) 619, 688, 770, 861
Hin6I GCGC 4 cut(s) 617, 686, 768, 859
HinP1I GCGC 4 cut(s) 617, 686, 768, 859
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 6 cut(s) 161, 195, 227, 305, 315, 715
HpaII CCGG 8 cut(s) 139, 165, 179, 319, 345, 653, 753, 777
HphI GGTGA 4 cut(s) 190, 202, 229, 667
Hpy166II GTNNAC 3 cut(s) 82, 127, 658
Hpy188I TCNGA 5 cut(s) 157, 268, 577, 714, 720
Hpy188III TCNNGA 8 cut(s) 35, 41, 49, 73, 184, 319, 386, 473
Hpy8I GTNNAC 3 cut(s) 82, 127, 658
Hpy99I CGWCG 3 cut(s) 161, 499, 541
HpyAV CCTTC 4 cut(s) 241, 334, 351, 690
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 3 cut(s) 791, 836, 890
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 174, 463, 676, 685, 767
HpyF3I CTNAG 2 cut(s) 283, 296
HpySE526I ACGT 1 cut(s) 78
HspAI GCGC 4 cut(s) 617, 686, 768, 859
Kpn2I TCCGGA 1 cut(s) 318
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 709
Kzo9I GATC 5 cut(s) 37, 51, 363, 577, 709
LmnI GCTCC 3 cut(s) 173, 532, 572
LweI GCATC 1 cut(s) 76
MaeI CTAG 3 cut(s) 429, 531, 672
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 2 cut(s) 590, 866
MalI GATC 5 cut(s) 39, 53, 365, 579, 711
MbiI CCGCTC 2 cut(s) 208, 527
MboI GATC 5 cut(s) 37, 51, 363, 577, 709
MboII GAAGA 7 cut(s) 100, 109, 241, 634, 748, 833, 836
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 1 cut(s) 752
MluCI AATT 2 cut(s) 703, 880
MlyI GAGTC 3 cut(s) 155, 221, 299
MmeI TCCRAC 2 cut(s) 180, 291
MroI TCCGGA 1 cut(s) 318
MroNI GCCGGC 1 cut(s) 138
MseI TTAA 1 cut(s) 372
MspI CCGG 8 cut(s) 139, 165, 179, 319, 345, 653, 753, 777
MspR9I CCNGG 2 cut(s) 179, 356
MvaI CCWGG 1 cut(s) 356
MvnI CGCG 2 cut(s) 474, 861
MwoI GCNNNNNNNGC 6 cut(s) 28, 174, 463, 676, 685, 767
NaeI GCCGGC 1 cut(s) 140
NciI CCSGG 1 cut(s) 179
NdeII GATC 5 cut(s) 37, 51, 363, 577, 709
NgoMIV GCCGGC 1 cut(s) 138
NlaIV GGNNCC 4 cut(s) 169, 292, 448, 751
NmuCI GTSAC 1 cut(s) 866
NruI TCGCGA 1 cut(s) 474
PasI CCCWGGG 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 497
PdiI GCCGGC 1 cut(s) 140
PfeI GAWTC 3 cut(s) 195, 315, 715
PinAI ACCGGT 1 cut(s) 652
PkrI GCNGC 2 cut(s) 24, 402
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 3 cut(s) 155, 221, 299
PpsI GAGTC 3 cut(s) 155, 221, 299
Psp6I CCWGG 1 cut(s) 354
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 4 cut(s) 169, 292, 448, 751
PspPI GGNCC 3 cut(s) 321, 668, 677
PsuI RGATCY 1 cut(s) 363
PvuI CGATCG 1 cut(s) 54
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 4 cut(s) 503, 584, 605, 818
RsaNI GTAC 4 cut(s) 502, 583, 604, 817
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 23, 401
Sau3AI GATC 5 cut(s) 37, 51, 363, 577, 709
Sau96I GGNCC 3 cut(s) 321, 668, 677
SchI GAGTC 3 cut(s) 155, 221, 299
ScrFI CCNGG 2 cut(s) 179, 356
SduI GDGCHC 1 cut(s) 752
SfaNI GCATC 1 cut(s) 76
SfiI GGCCNNNNNGGCC 1 cut(s) 676
SinI GGWCC 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 71, 101
SmoI CTYRAG 2 cut(s) 71, 101
Sse9I AATT 2 cut(s) 703, 880
SspMI CTAG 3 cut(s) 429, 531, 672
StyD4I CCNGG 2 cut(s) 177, 354
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 1 cut(s) 202
TaiI ACGT 1 cut(s) 81
TaqI TCGA 7 cut(s) 36, 42, 243, 308, 385, 645, 701
TaqII GACCGA 1 cut(s) 663
TasI AATT 2 cut(s) 703, 880
TauI GCSGC 2 cut(s) 25, 403
TfiI GAWTC 3 cut(s) 195, 315, 715
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 3 cut(s) 619, 777, 798
TseFI GTSAC 1 cut(s) 866
Tsp45I GTSAC 1 cut(s) 866
TspGWI ACGGA 5 cut(s) 326, 356, 456, 525, 570
TspRI CASTG 3 cut(s) 619, 777, 798
VpaK11BI GGWCC 1 cut(s) 321
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 3 cut(s) 429, 531, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.