pycom01g16640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001015
Physical Location & Seq
Forward (+)
16152166 .. 16153717
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16640.3

Sequence Viewer

Length: 606 bp
ATGCCAGACCTAGACGCCACTATTTCCTATATATACATGCATCAACTTTCATCTCCGACTCATACACAAACTCTTCTTGGTTTTTATTTTTCATACAGTTTGGCTGCGACAAGGATGAAATCTGCAGATGAAGAAGAAGAGCACGACTCACACCGGCAAGCCCTAAAGAGGAAATCTGCAGAAGAAGAAGAAGAGTATGACTCATACCGACACGTAGTTTTAAAGGGTGAGAGAGTTCTCAAACATTGTGTGGAGAAACAGGAATACACTATTATATCCAGTCCCATCTCTTTTTGGTCTTTAATTTTTCTATACAGCTTGACCATGGCAAGGAGGATGAACAATGATGTAGATAACTACAACAGAGCTCTAAAGAAGAAAAGGATGACAAACAAGGATGATGGACAAGAAAAACACTCATACTTGGACCAAAAAGTCGTCCTGAGTGTTGACATCTACCATGCCAAAGGTCTCGATGGCCCTAATTATAGTACAATACGTTGTACTAATAGGGTCTATCAACTTGTCTTACGGATGGAACCTGGCAAAGAGTACGAGTCGAAGTTTGTCTACAGTTGCCGAACCCTAAATGGAATCTACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

202

Amino Acids

23.65

Weight (kDa)

7.72

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 570
AcyI GRCGYC 1 cut(s) 15
AfaI GTAC 3 cut(s) 493, 505, 554
AfiI CCNNNNNNNGG 2 cut(s) 168, 330
AflIII ACRYGT 1 cut(s) 211
AjnI CCWGG 1 cut(s) 541
AluBI AGCT 2 cut(s) 318, 368
AluI AGCT 2 cut(s) 318, 368
Alw21I GWGCWC 2 cut(s) 144, 370
Alw26I GTCTC 1 cut(s) 476
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 427, 479
AsuHPI GGTGA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 370
BarI GAAGNNNNNNTAC 2 cut(s) 554, 586
Bbv12I GWGCWC 2 cut(s) 144, 370
BbvI GCAGC 1 cut(s) 91
BccI CCATC 4 cut(s) 293, 395, 470, 529
BciT130I CCWGG 1 cut(s) 543
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 1 cut(s) 11
BfmI CTRYAG 3 cut(s) 123, 177, 571
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 427, 479
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 1 cut(s) 543
BmsI GCATC 1 cut(s) 49
BplI GAGNNNNNCTC 4 cut(s) 131, 163, 185, 217
BsaAI YACGTR 1 cut(s) 214
BsaHI GRCGYC 1 cut(s) 15
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 324
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 330
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 5 cut(s) 120, 342, 390, 403, 540
BseLI CCNNNNNNNGG 2 cut(s) 168, 330
BseMII CTCAG 1 cut(s) 434
BseNI ACTGG 1 cut(s) 279
BseXI GCAGC 1 cut(s) 91
BshFI GGCC 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 144, 370
BsiSI CCGG 1 cut(s) 154
BslFI GGGAC 1 cut(s) 267
BslI CCNNNNNNNGG 2 cut(s) 168, 330
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 267
BsnI GGCC 1 cut(s) 480
Bso31I GGTCTC 1 cut(s) 476
Bsp1286I GDGCHC 2 cut(s) 144, 370
Bsp19I CCATGG 1 cut(s) 324
BspANI GGCC 1 cut(s) 480
BspCNI CTCAG 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 540
BspMAI CTGCAG 2 cut(s) 127, 181
BspQI GCTCTTC 1 cut(s) 132
BspTNI GGTCTC 1 cut(s) 476
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 279
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 1 cut(s) 324
BssNI GRCGYC 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 2 cut(s) 98, 575
Bst6I CTCTTC 3 cut(s) 78, 132, 186
BstACI GRCGYC 1 cut(s) 15
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 5 cut(s) 120, 342, 390, 403, 540
BstMAI GTCTC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 543
BstNSI RCATGY 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 541
BstSFI CTRYAG 3 cut(s) 123, 177, 571
BstV1I GCAGC 1 cut(s) 91
BsuRI GGCC 1 cut(s) 480
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 5 cut(s) 120, 342, 390, 403, 540
Cac8I GCNNGC 1 cut(s) 159
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 427, 479
CseI GACGC 1 cut(s) 23
Csp6I GTAC 3 cut(s) 492, 504, 553
CviAII CATG 3 cut(s) 37, 325, 461
CviJI RGCY 5 cut(s) 104, 161, 318, 368, 480
CviKI_1 RGCY 5 cut(s) 104, 161, 318, 368, 480
CviQI GTAC 3 cut(s) 492, 504, 553
DdeI CTNAG 1 cut(s) 443
DraI TTTAAA 1 cut(s) 222
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
Eam1104I CTCTTC 3 cut(s) 78, 132, 186
EarI CTCTTC 3 cut(s) 78, 132, 186
Ecl136II GAGCTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 324
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 40, 328, 464
FaqI GGGAC 1 cut(s) 267
FatI CATG 3 cut(s) 36, 324, 460
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 5 cut(s) 127, 349, 397, 410, 547
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 1 cut(s) 11
GluI GCNGC 1 cut(s) 105
HaeIII GGCC 1 cut(s) 480
HapII CCGG 1 cut(s) 154
HgaI GACGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 15
Hin1II CATG 3 cut(s) 40, 328, 464
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 5 cut(s) 58, 146, 200, 557, 594
HpaII CCGG 1 cut(s) 154
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 451, 571
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 2 cut(s) 442, 473
Hpy8I GTNNAC 2 cut(s) 451, 571
HpyCH4III ACNGT 2 cut(s) 98, 575
HpyCH4IV ACGT 2 cut(s) 213, 499
HpyCH4V TGCA 3 cut(s) 40, 125, 179
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 2 cut(s) 213, 499
Hsp92I GRCGYC 1 cut(s) 15
Hsp92II CATG 3 cut(s) 40, 328, 464
LguI GCTCTTC 1 cut(s) 132
LpnPI CCDG 7 cut(s) 18, 167, 245, 292, 455, 528, 555
Lsp1109I GCAGC 1 cut(s) 91
LweI GCATC 1 cut(s) 49
MaeI CTAG 1 cut(s) 11
MaeII ACGT 2 cut(s) 213, 499
MboII GAAGA 9 cut(s) 65, 143, 146, 149, 194, 197, 200, 203, 388
MhlI GDGCHC 2 cut(s) 144, 370
MluCI AATT 2 cut(s) 303, 484
MlyI GAGTC 4 cut(s) 52, 140, 194, 566
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 2 cut(s) 162, 327
Mph1103I ATGCAT 1 cut(s) 42
MseI TTAA 2 cut(s) 221, 302
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 543
MvaI CCWGG 1 cut(s) 543
NcoI CCATGG 1 cut(s) 324
NlaIII CATG 3 cut(s) 40, 328, 464
NlaIV GGNNCC 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 42
NspI RCATGY 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 594
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 4 cut(s) 52, 140, 194, 565
PpsI GAGTC 4 cut(s) 52, 140, 194, 565
Ppu21I YACGTR 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 540
PspPI GGNCC 2 cut(s) 427, 479
PstI CTGCAG 2 cut(s) 127, 181
RsaI GTAC 3 cut(s) 493, 505, 554
RsaNI GTAC 3 cut(s) 492, 504, 553
SacI GAGCTC 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 221, 302
SatI GCNGC 1 cut(s) 105
Sau96I GGNCC 2 cut(s) 427, 479
SchI GAGTC 4 cut(s) 52, 140, 194, 566
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 2 cut(s) 144, 370
SetI ASST 7 cut(s) 12, 216, 320, 370, 472, 502, 544
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 3 cut(s) 123, 177, 571
SinI GGWCC 1 cut(s) 427
Sse9I AATT 2 cut(s) 303, 484
SspMI CTAG 1 cut(s) 11
SstI GAGCTC 1 cut(s) 370
StyD4I CCNGG 1 cut(s) 541
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 98, 575
TaiI ACGT 2 cut(s) 216, 502
TaqI TCGA 2 cut(s) 474, 560
TasI AATT 2 cut(s) 303, 484
TatI WGTACW 2 cut(s) 491, 503
TfiI GAWTC 1 cut(s) 594
Tru1I TTAA 2 cut(s) 221, 302
Tru9I TTAA 2 cut(s) 221, 302
TseI GCWGC 1 cut(s) 104
TspDTI ATGAA 5 cut(s) 39, 81, 131, 144, 353
TspGWI ACGGA 1 cut(s) 547
VpaK11BI GGWCC 1 cut(s) 427
XceI RCATGY 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 570
XspI CTAG 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.