Prupe.2G246600_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001015
Physical Location & Seq
Forward (+)
26322626 .. 26323883
1258 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G246600.1

Sequence Viewer

Length: 948 bp
ATGGCCACCTCTCGCCCTCCACCGCCGAAGCATCTCGATCTCGACCTTACGATCGTCTCAGCCAAGCATCTAAAGAACGTCAACTGGAAAAACGGCGACCTCAAGCCCTACGCGGTCTTCTGGGTCGACTCAGACCGCCGACTCGCCACCAAATCTGACGACTCCGGCTCAACCCGCCCGGTCTGGAACGAGCGGTTCACCGTCCCACTCACCCTCCCCGTCCACGACTCCTTCCTCACCCTCGAAATCTTCCACTCCAAACCCTCCGACACTCCCAAGCCCTTAGTCGGCACCCTCCGGGTCCCACTCAAGGACCTGCCCGACCCGGATGACTCCACTCGAATCCGCACCTTCCAGCTCGTCCGCCCCTCGGGCCGTCCACAAGGTAAGATCCGCGTAAAGCTCGCCGTTCGAGAACGACCTCTGCCGCCAGATTACCATATGACCCCTCCGCCCAGCTATTTTTACTCAGGCGCCCCTATGCCTCCACCCTCTGTTCGTGACTACAGGTCATACTCGCCGTCGCCGTACTCTTCTCTACAAGCTTCGGCTCCGACTCCATCGGCTTCCCCACCGCCGCCGCCGCCGTACCATTACAACTCGTATTCCGATCCGTACTCGAGTTACTATCCGGCGTACTACTCCAGCGCGCCACCTCCGCCTCCACCGAGGCCGTTCTTCGACCGGCAGGCAGGTTACAGTGGGCCGGGTGGTCCTGGTGGGCCTGGCGGGCCTTCCGCACCACTGGACTACTCGAATTATGATCAGAAGCCCAAGAGTGGGAAGATGGGGCTGGGCACGGGATTGGCTGTGGGCGCAGTGGCGGGAGGTATAGGTGGACTGGCATTGGATGAGGGATTGAGGTACGAAGAAGATAAGATTGCGGAGAGGGTTGAGAATGAGTTGCGTGAGCGTGACGATTACAGCAACTATCGTGCCGATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
C2

Protein Analysis

316

Amino Acids

34.71

Weight (kDa)

8.76

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 324, 683
AccB1I GGYRCC 2 cut(s) 290, 473
AccBSI CCGCTC 1 cut(s) 193
AccI GTMKAC 1 cut(s) 126
AccII CGCG 3 cut(s) 113, 396, 650
AclWI GGATC 2 cut(s) 385, 605
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 5 cut(s) 530, 590, 617, 638, 866
AfiI CCNNNNNNNGG 5 cut(s) 287, 310, 370, 779, 780
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 2 cut(s) 715, 724
AluBI AGCT 4 cut(s) 358, 403, 459, 545
AluI AGCT 4 cut(s) 358, 403, 459, 545
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 2 cut(s) 385, 605
Ama87I CYCGRG 2 cut(s) 370, 619
AoxI GGCC 6 cut(s) 3, 373, 671, 704, 722, 731
AspLEI GCGC 4 cut(s) 476, 650, 652, 818
AspS9I GGNCC 7 cut(s) 301, 313, 373, 704, 713, 722, 731
AsuC2I CCSGG 4 cut(s) 179, 299, 326, 708
AsuHPI GGTGA 3 cut(s) 190, 202, 229
AvaI CYCGRG 2 cut(s) 370, 619
AvaII GGWCC 3 cut(s) 301, 313, 713
BaeGI GKGCMC 1 cut(s) 800
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 290, 473
BbsI GAAGAC 1 cut(s) 109
BccI CCATC 2 cut(s) 568, 781
BceAI ACGGC 7 cut(s) 109, 360, 392, 505, 511, 571, 658
BciT130I CCWGG 2 cut(s) 717, 726
BclI TGATCA 1 cut(s) 763
BcnI CCSGG 4 cut(s) 179, 299, 326, 708
BcoDI GTCTC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 477
BfuAI ACCTGC 2 cut(s) 324, 683
BglI GCCNNNNNGGC 2 cut(s) 372, 730
BisI GCNGC 4 cut(s) 428, 578, 581, 584
BlsI GCNGC 4 cut(s) 429, 579, 582, 585
Bme1390I CCNGG 6 cut(s) 179, 299, 326, 708, 717, 726
Bme18I GGWCC 3 cut(s) 301, 313, 713
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 2 cut(s) 370, 619
BmgT120I GGNCC 7 cut(s) 301, 313, 373, 704, 713, 722, 731
BmiI GGNNCC 5 cut(s) 292, 302, 303, 475, 552
BmrFI CCNGG 6 cut(s) 179, 299, 326, 708, 717, 726
BmsI GCATC 2 cut(s) 40, 76
BpiI GAAGAC 1 cut(s) 109
BpmI CTGGAG 1 cut(s) 628
BpuEI CTTGAG 2 cut(s) 86, 293
BpuMI CCSGG 4 cut(s) 179, 299, 326, 708
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 2 cut(s) 369, 668
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 5 cut(s) 287, 310, 370, 779, 780
Bse118I RCCGGY 1 cut(s) 684
Bse1I ACTGG 3 cut(s) 89, 750, 846
BseBI CCWGG 2 cut(s) 717, 726
BseDI CCNNGG 2 cut(s) 369, 668
BseGI GGATG 2 cut(s) 334, 856
BseLI CCNNNNNNNGG 5 cut(s) 287, 310, 370, 779, 780
BseMII CTCAG 3 cut(s) 72, 144, 483
BseNI ACTGG 3 cut(s) 89, 750, 846
BsePI GCGCGC 1 cut(s) 648
BseSI GKGCMC 1 cut(s) 800
BseYI CCCAGC 2 cut(s) 455, 793
Bsh1236I CGCG 3 cut(s) 113, 396, 650
Bsh1285I CGRYCG 2 cut(s) 54, 685
BshFI GGCC 6 cut(s) 5, 375, 673, 706, 724, 733
BshNI GGYRCC 2 cut(s) 290, 473
BsiEI CGRYCG 2 cut(s) 54, 685
BsiHKCI CYCGRG 2 cut(s) 370, 619
BsiSI CCGG 7 cut(s) 165, 179, 298, 326, 632, 685, 707
BslFI GGGAC 2 cut(s) 188, 287
BslI CCNNNNNNNGG 5 cut(s) 287, 310, 370, 779, 780
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 188, 287
BsnI GGCC 6 cut(s) 5, 375, 673, 706, 724, 733
BsoBI CYCGRG 2 cut(s) 370, 619
Bsp1286I GDGCHC 1 cut(s) 800
Bsp143I GATC 5 cut(s) 37, 51, 390, 610, 763
BspANI GGCC 6 cut(s) 5, 375, 673, 706, 724, 733
BspCNI CTCAG 3 cut(s) 71, 143, 482
BspFNI CGCG 3 cut(s) 113, 396, 650
BspLI GGNNCC 5 cut(s) 292, 302, 303, 475, 552
BspMI ACCTGC 2 cut(s) 324, 683
BspPI GGATC 2 cut(s) 385, 605
BspT107I GGYRCC 2 cut(s) 290, 473
BsrBI CCGCTC 1 cut(s) 193
BsrFI RCCGGY 1 cut(s) 684
BsrI ACTGG 3 cut(s) 89, 750, 846
BssAI RCCGGY 1 cut(s) 684
BssECI CCNNGG 2 cut(s) 369, 668
BssHII GCGCGC 1 cut(s) 648
BssMI GATC 5 cut(s) 37, 51, 390, 610, 763
BssNI GRCGYC 1 cut(s) 474
Bst2UI CCWGG 2 cut(s) 717, 726
Bst4CI ACNGT 2 cut(s) 202, 701
Bst6I CTCTTC 1 cut(s) 538
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 4 cut(s) 405, 650, 690, 731
BstDEI CTNAG 4 cut(s) 58, 130, 283, 469
BstF5I GGATG 2 cut(s) 334, 856
BstFNI CGCG 3 cut(s) 113, 396, 650
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 4 cut(s) 476, 650, 652, 818
BstKTI GATC 5 cut(s) 40, 54, 393, 613, 766
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 5 cut(s) 37, 51, 390, 610, 763
BstMCI CGRYCG 2 cut(s) 54, 685
BstMWI GCNNNNNNNGC 6 cut(s) 174, 372, 583, 658, 730, 815
BstNI CCWGG 2 cut(s) 717, 726
BstSCI CCNGG 6 cut(s) 177, 297, 324, 706, 715, 724
BstSFI CTRYAG 1 cut(s) 505
BstSLI GKGCMC 1 cut(s) 800
BstUI CGCG 3 cut(s) 113, 396, 650
BstV2I GAAGAC 1 cut(s) 109
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BsuRI GGCC 6 cut(s) 5, 375, 673, 706, 724, 733
BtsCI GGATG 2 cut(s) 334, 856
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 3 cut(s) 706, 743, 825
BveI ACCTGC 2 cut(s) 324, 683
Cac8I GCNNGC 4 cut(s) 405, 650, 690, 731
CfoI GCGC 4 cut(s) 476, 650, 652, 818
Cfr10I RCCGGY 1 cut(s) 684
Cfr13I GGNCC 7 cut(s) 301, 313, 373, 704, 713, 722, 731
Csp6I GTAC 5 cut(s) 529, 589, 616, 637, 865
CviQI GTAC 5 cut(s) 529, 589, 616, 637, 865
DdeI CTNAG 4 cut(s) 58, 130, 283, 469
DinI GGCGCC 1 cut(s) 475
DpnI GATC 5 cut(s) 39, 53, 392, 612, 765
DpnII GATC 5 cut(s) 37, 51, 390, 610, 763
DriI GACNNNNNGTC 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 538
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 538
EciI GGCGGA 3 cut(s) 353, 441, 648
Eco47I GGWCC 3 cut(s) 301, 313, 713
Eco88I CYCGRG 2 cut(s) 370, 619
EcoO109I RGGNCCY 2 cut(s) 301, 313
EcoRII CCWGG 2 cut(s) 715, 724
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
Esp3I CGTCTC 1 cut(s) 61
FaiI YATR 6 cut(s) 441, 443, 482, 514, 762, 833
FaqI GGGAC 2 cut(s) 188, 287
FauI CCCGC 3 cut(s) 182, 722, 817
FauNDI CATATG 1 cut(s) 441
FbaI TGATCA 1 cut(s) 763
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 428, 578, 581, 584
FokI GGATG 2 cut(s) 341, 863
Fsp4HI GCNGC 4 cut(s) 428, 578, 581, 584
GlaI GCGC 4 cut(s) 475, 649, 651, 817
GluI GCNGC 4 cut(s) 428, 578, 581, 584
GsaI CCCAGC 2 cut(s) 459, 797
GsuI CTGGAG 1 cut(s) 628
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 6 cut(s) 5, 375, 673, 706, 724, 733
HapII CCGG 7 cut(s) 165, 179, 298, 326, 632, 685, 707
HhaI GCGC 4 cut(s) 476, 650, 652, 818
Hin1I GRCGYC 1 cut(s) 474
Hin6I GCGC 4 cut(s) 474, 648, 650, 816
HinP1I GCGC 4 cut(s) 474, 648, 650, 816
HincII GTYRAC 2 cut(s) 82, 127
HindII GTYRAC 2 cut(s) 82, 127
HindIII AAGCTT 1 cut(s) 543
HinfI GANTC 7 cut(s) 128, 141, 161, 227, 332, 342, 556
HpaII CCGG 7 cut(s) 165, 179, 298, 326, 632, 685, 707
HphI GGTGA 3 cut(s) 190, 202, 229
Hpy166II GTNNAC 6 cut(s) 82, 127, 198, 223, 380, 839
Hpy188I TCNGA 6 cut(s) 133, 157, 268, 555, 610, 768
Hpy188III TCNNGA 5 cut(s) 35, 41, 184, 413, 500
Hpy8I GTNNAC 6 cut(s) 82, 127, 198, 223, 380, 839
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 3 cut(s) 241, 361, 744
HpyCH4III ACNGT 2 cut(s) 202, 701
HpyCH4IV ACGT 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 6 cut(s) 174, 372, 583, 658, 730, 815
HpyF3I CTNAG 4 cut(s) 58, 130, 283, 469
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 1 cut(s) 474
HspAI GCGC 4 cut(s) 474, 648, 650, 816
KasI GGCGCC 1 cut(s) 473
KflI GGGWCCC 1 cut(s) 301
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 5 cut(s) 37, 51, 390, 610, 763
LmnI GCTCC 1 cut(s) 556
LweI GCATC 2 cut(s) 40, 76
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 4 cut(s) 500, 623, 695, 914
MalI GATC 5 cut(s) 39, 53, 392, 612, 765
MbiI CCGCTC 1 cut(s) 193
MboI GATC 5 cut(s) 37, 51, 390, 610, 763
MboII GAAGA 7 cut(s) 109, 241, 525, 670, 796, 881, 884
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 1 cut(s) 800
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 757
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 6 cut(s) 122, 135, 155, 221, 326, 550
MmeI TCCRAC 2 cut(s) 291, 578
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 7 cut(s) 165, 179, 298, 326, 632, 685, 707
MspR9I CCNGG 6 cut(s) 179, 299, 326, 708, 717, 726
MvaI CCWGG 2 cut(s) 717, 726
MvnI CGCG 3 cut(s) 113, 396, 650
MwoI GCNNNNNNNGC 6 cut(s) 174, 372, 583, 658, 730, 815
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 4 cut(s) 179, 299, 326, 708
NdeI CATATG 1 cut(s) 441
NdeII GATC 5 cut(s) 37, 51, 390, 610, 763
NlaIV GGNNCC 5 cut(s) 292, 302, 303, 475, 552
NmuCI GTSAC 2 cut(s) 500, 914
PaeR7I CTCGAG 1 cut(s) 619
PauI GCGCGC 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 524
PfeI GAWTC 1 cut(s) 342
PkrI GCNGC 4 cut(s) 429, 579, 582, 585
Ple19I CGATCG 1 cut(s) 54
PleI GAGTC 6 cut(s) 122, 135, 155, 221, 326, 550
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 6 cut(s) 122, 135, 155, 221, 326, 550
PpuMI RGGWCCY 2 cut(s) 301, 313
Psp5II RGGWCCY 2 cut(s) 301, 313
Psp6I CCWGG 2 cut(s) 715, 724
PspFI CCCAGC 2 cut(s) 455, 793
PspGI CCWGG 2 cut(s) 715, 724
PspN4I GGNNCC 5 cut(s) 292, 302, 303, 475, 552
PspPI GGNCC 7 cut(s) 301, 313, 373, 704, 713, 722, 731
PspPPI RGGWCCY 2 cut(s) 301, 313
PspXI VCTCGAGB 1 cut(s) 619
PsuI RGATCY 1 cut(s) 390
PteI GCGCGC 1 cut(s) 648
PvuI CGATCG 1 cut(s) 54
RsaI GTAC 5 cut(s) 530, 590, 617, 638, 866
RsaNI GTAC 5 cut(s) 529, 589, 616, 637, 865
SalI GTCGAC 1 cut(s) 125
SatI GCNGC 4 cut(s) 428, 578, 581, 584
Sau3AI GATC 5 cut(s) 37, 51, 390, 610, 763
Sau96I GGNCC 7 cut(s) 301, 313, 373, 704, 713, 722, 731
SchI GAGTC 6 cut(s) 122, 135, 155, 221, 326, 550
ScrFI CCNGG 6 cut(s) 179, 299, 326, 708, 717, 726
SduI GDGCHC 1 cut(s) 800
SfaNI GCATC 2 cut(s) 40, 76
SfcI CTRYAG 1 cut(s) 505
SfiI GGCCNNNNNGGCC 1 cut(s) 730
SfoI GGCGCC 1 cut(s) 475
Sfr274I CTCGAG 1 cut(s) 619
SinI GGWCC 3 cut(s) 301, 313, 713
SlaI CTCGAG 1 cut(s) 619
SmlI CTYRAG 3 cut(s) 101, 308, 619
SmoI CTYRAG 3 cut(s) 101, 308, 619
Sse9I AATT 1 cut(s) 757
SspDI GGCGCC 1 cut(s) 473
StyD4I CCNGG 6 cut(s) 177, 297, 324, 706, 715, 724
TaaI ACNGT 2 cut(s) 202, 701
TaiI ACGT 1 cut(s) 81
TaqI TCGA 9 cut(s) 36, 42, 126, 243, 340, 412, 620, 681, 755
TasI AATT 1 cut(s) 757
TauI GCSGC 4 cut(s) 430, 580, 583, 586
TfiI GAWTC 1 cut(s) 342
TscAI CASTG 3 cut(s) 706, 750, 825
TseFI GTSAC 2 cut(s) 500, 914
Tsp45I GTSAC 2 cut(s) 500, 914
TspGWI ACGGA 1 cut(s) 603
TspRI CASTG 3 cut(s) 706, 750, 825
VpaK11BI GGWCC 3 cut(s) 301, 313, 713
XhoI CTCGAG 1 cut(s) 619
XmiI GTMKAC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.