MD01G1173400.v1.1

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000527
Physical Location & Seq
Reverse (-)
27508856 .. 27515156
6301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1173400.v1.1.491

Sequence Viewer

Length: 3066 bp
ATGGTGGTCAAGAAAGGTCAATCAGCAACCCATTTGGGCAAGGATTCAAAGGTGGAAGTTTGCAGCAATGAAGATGGCTTCAAGGGCGCATGGTTCCCCGCCAAAATAATCGACTCGAAACCACTGATTCCGGCGACGAAGAAGAAAAGGAAGAGCTTTTCCGACGGCAGCAGCAACTCCTTCTCACCCACCAAGGCTGAGGTCCAGTACGAGACTCTGGTCTCTGACGTCGACCCGGACAAGCCCCTGACGGAGTTTGTCGAGTTGGGTCAAATCCGGCCGGTGCCTCCGGACGACAATGTCGACAGGCCCTTCGAGCCCGGCGACAAAGTCGACGCCTTTCACCTCGATGCGTGGTGGCCCGGGGTTGTGATCAAGCGCGAGGAGGATGCGTACACGGTGGGGTTCATGTACCCGCCTGATCTGCTTGTGCTGCGGCGGTCAGAATTGCGTTCTCACTGGGATTTGGCGGACGGCGTTTGGGTCCGAGCCAAAAAGGAGATGATGATGGTATCAAATTTCAGCCCTGGGACAGCAGTTGAAATGAATCCGGACGAAGAAAGACTATTTTATGCTTGGTTTCCAGCAATTTATCTTGGTCAACTGGGGATCGACTCTTTCTTGCTTCAATATAAGAAATCAAACAACAGCGATGATAAAATTGTCGTACGTGGCCATCAGATTCGGCCTCAGCCTCCAAATTCGACTGAAAGAGATTTTAACTTGTTGGATAAGGTGGATGCATTCCATGGCATGGGTTGGTGGGTCGGAGACATTACAAAAGTTCTTAAAGGAAACAAGTATGCGGTCACTTTAAGTTGCACGAGGCAGGAGAAGGAATTCAGCCTTTCAGAATTGAGGACTCACATGGATTGGACTGATGGAGGATGGGCTACTATGAGCCATGGAAAATGGATTACTGAAGTTCGGGAATTTCACTTTAGAAGGGGGTCTGAAGCACAATCAAAGCATTCATGTCAGAGTTCCAAACGTGATTATGAATTACAATCATGTGAGAGTTACGGAAACTCTGATGTAGGTACACCACTTCGAAAGAAAACACCTTGTTCTAGGATCTCAGTGAAAATTCAATCGAAGCCCTTGAGTCCTTGCACGGATAAATTACCTTATGGAAAGACCAACAAGAAATTGAAAATGTCTCCGCAACCTAAGGATGATGCAACAATTTTAAGTCTTTACAAGAAGTTAAAAGGTGGGCATTCAGATGACTCCTTATCTTTGGAGAAACCGTTTGCAAGGAGAACGCTGGTTAAAACTCTAAACAAAGAATCCCCAGTTATTAATGTTTTGAAGATTGCGAAACTGAAGGTTAGAGGACTAGATGATCAAGCATTAACTCATTTCAAAAGAAAAGGCAAGAAAAGAAGTGAAATTGAAAAGACCGGTGAAGTAAATATTGGAGATGAGCATGTAATGGACAACACTGAATCTTCTGGTATCAAGTCAGAGTCTGAAGCAACAGGGGGATCTCATGCTGGAGCATCATGTCTGCTCCCTATGGAGGGGGTTGGACAGAATGAAGATGGAGTGACCAGTGCAGTAGAAGGGAATGGAAAACGGACTGCATTGCTTGTTGAAGGCGAGGAACATAATGGCGGTGGATCTATGGCTGCGGATTGCTCGAATGATATATTCGAATTGTCTATGATCACAAGGTTGGATTTGAAAGGTGAGAAACATGATGGAACTGGAGTTGTTGCTCAAGTTGAATCGACTGAGACTCAAGTTGCTAAATATAAAGGTTCTGAAGATGCAATGGTCGAAGAGGAAGCTGTATATTCTGCAATGGATATCGATAAAGGCATTACAAGTATTACAGTGGGCTCAAGTAACCCTGCTGGGATTTCCGAACAACAAAGTGAGGTCAGAAAACAGGTTGAAACTGGCGTAACAGGTTCTGAAGAAGCAATGAGAGAGACGGAAACTGTGAATTCTACAACGGATTACTTGACACAAGAAGGTCAGGTGGTTGTGACTGGAGTATTGCCAAAGCCATCCGCTCATAATCAACCTTTATCACTGTGCATAAATGAAGTGCATTCTGTAAAAACCATAGGTGGTACAAGTAACCTTGTTGGGACTGCCAACCAAGAGATTAATGGAAGACAAGTTGAAATTAGTGTCACAGGTAACCTTGCTGGGACTCCGAACCAACAAAACGAGGTTAATGGAAGACAAGTTGAAACTGGTGTCACAGATGAGGCTGATGCGGAGCAAAACAACATGAGACAGGCAGAAACTGTTGATTCTCCAATGGGTTGCTCGACAAAAGAACTTCAGGTGGCTGTGACTGGAATATCCGAAAAGGCATCTGCTCATGATCAACCCTTTTTGTTGTGCACAAGTGAAATGCATTCTGTCAAACCTACAGAGGGCTCAAGTAACCCAGCCGAGACTGATAACCAACAAAATGAGGTTACAGGTACACAAGTTGAAACTGGTGTCACTGTTAACATTGAGCAACAAGACAGTCCAGATTTGCCATTTGTGAAATCTTATCCTGAAATTTGGGAAAAAGTTGAATCTATGGATGCATTTAGAAGATATCCGCAAAAACCACATTTTTATCCTTTAGTTAAGTGTGGAGAGCTAAGTCGCGAATCATTAGCCATTGCAAAGATGTTAACCTTTGCCTCTTTGGTTGAGAAGACCTCCAAGGTGAACATTGAGGTTCCTGACGACTTCTTTGATAGCAGCTTCCAAGAAATAGGTGATTTAGAAACGTTTGGATTTGATGTCAAGGCAGTACGTGACCGTCTGAACTCTTTGCTAGAAATGAAAGTTCAGTTGGGGCAGCTTCAGGACAAGTCAAAGGAAGTTGAAATTCAGATTGCTGTGCAAACTCAAGAAAGAAAGAAACATAATGAAAAAATCAGTGGGGTTGCTAAGCAGATTAAGGACTTGCAGGATCAACTGGTGGTGCTGATGTCAGAGGACGCGGCCAAAGGTACTGAAATTAGCAGGTTGCAATCCGAAGAAAACGCAATCACTGAAAGCATTCTGAGCACCCGTCGTAACTTTGAGAAACTGAGCGAAGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

1022

Amino Acids

112.95

Weight (kDa)

5.3

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 97 5e-14 Agenet domain
Agenet PF05641 177 - 233 4e-06 Agenet domain
DUF724 PF05266 834 - 1018 8.1e-48 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AatII GACGTC 1 cut(s) 231
Acc36I ACCTGC 1 cut(s) 2973
AccB1I GGYRCC 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 754
AccBSI CCGCTC 1 cut(s) 2021
AccI GTMKAC 3 cut(s) 231, 303, 333
AccII CGCG 3 cut(s) 381, 2619, 2960
AccIII TCCGGA 2 cut(s) 289, 550
AclI AACGTT 1 cut(s) 2744
AclWI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2937
AcoI YGGCCR 3 cut(s) 278, 673, 2961
AcsI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2526, 2844
AcuI CTGAAG 8 cut(s) 942, 975, 1346, 1494, 1788, 1941, 2282, 2804
AcyI GRCGYC 2 cut(s) 228, 336
AfaI GTAC 9 cut(s) 209, 395, 413, 669, 1042, 2083, 2446, 2769, 2971
AfiI CCNNNNNNNGG 4 cut(s) 754, 1522, 1523, 2393
AgeI ACCGGT 1 cut(s) 1403
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 4 cut(s) 1401, 1433, 2792, 2824
AluBI AGCT 5 cut(s) 156, 1793, 2611, 2718, 2818
AluI AGCT 5 cut(s) 156, 1793, 2611, 2718, 2818
Alw21I GWGCWC 2 cut(s) 2363, 3029
Alw26I GTCTC 8 cut(s) 206, 226, 765, 1164, 1733, 1931, 2242, 2408
Alw44I GTGCAC 1 cut(s) 2359
AlwI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2937
AlwNI CAGNNNCTG 3 cut(s) 1472, 1919, 2261
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 2 cut(s) 289, 550
AoxI GGCC 6 cut(s) 278, 308, 359, 673, 686, 2961
ApaLI GTGCAC 1 cut(s) 2359
ApeKI GCWGC 7 cut(s) 63, 168, 171, 433, 1631, 2715, 2815
ApoI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2526, 2844
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 2690, 2722
AseI ATTAAT 2 cut(s) 1302, 2118
AsiGI ACCGGT 1 cut(s) 1403
Asp700I GAANNNNTTC 2 cut(s) 839, 3018
AspLEI GCGC 2 cut(s) 89, 381
AspS9I GGNCC 4 cut(s) 202, 309, 360, 484
AsuC2I CCSGG 4 cut(s) 236, 321, 363, 364
AsuHPI GGTGA 6 cut(s) 177, 335, 1418, 1703, 2692, 2744
AsuII TTCGAA 2 cut(s) 1051, 1656
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 2 cut(s) 202, 484
AxyI CCTNAGG 1 cut(s) 1170
BaeGI GKGCMC 1 cut(s) 2363
BaeI ACNNNNGTAYC 2 cut(s) 2436, 2469
BalI TGGCCA 1 cut(s) 675
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 3 cut(s) 321, 1847, 2399
BauI CACGAG 1 cut(s) 823
BbsI GAAGAC 3 cut(s) 2131, 2200, 2675
Bbv12I GWGCWC 2 cut(s) 2363, 3029
BbvCI CCTCAGC 2 cut(s) 198, 690
BbvI GCAGC 7 cut(s) 75, 180, 183, 420, 1618, 2727, 2827
BccI CCATC 8 cut(s) 68, 502, 684, 875, 882, 1538, 1697, 2023
BceAI ACGGC 2 cut(s) 181, 490
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 4 cut(s) 372, 1345, 1668, 2341
BcnI CCSGG 4 cut(s) 236, 321, 363, 364
BcoDI GTCTC 8 cut(s) 206, 226, 765, 1164, 1733, 1931, 2242, 2408
BfaI CTAG 3 cut(s) 1071, 1340, 2792
BfmI CTRYAG 1 cut(s) 2388
BfuAI ACCTGC 1 cut(s) 2973
BisI GCNGC 9 cut(s) 64, 169, 172, 434, 437, 1632, 2716, 2816, 2961
BlpI GCTNAGC 1 cut(s) 2907
BlsI GCNGC 9 cut(s) 65, 170, 173, 435, 438, 1633, 2717, 2817, 2962
Bme1390I CCNGG 5 cut(s) 236, 321, 363, 364, 528
Bme18I GGWCC 2 cut(s) 202, 484
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 4 cut(s) 202, 309, 360, 484
BmiI GGNNCC 4 cut(s) 95, 285, 485, 2694
BmrFI CCNGG 5 cut(s) 236, 321, 363, 364, 528
BmrI ACTGGG 3 cut(s) 469, 614, 1289
BmsI GCATC 9 cut(s) 340, 379, 730, 1168, 1511, 1762, 2218, 2339, 2542
BmuI ACTGGG 3 cut(s) 469, 614, 1289
BoxI GACNNNNGTC 1 cut(s) 218
BpiI GAAGAC 3 cut(s) 2131, 2200, 2675
BplI GAGNNNNNCTC 2 cut(s) 2657, 2689
BpmI CTGGAG 3 cut(s) 1518, 1731, 2019
Bpu10I CCTNAGC 2 cut(s) 198, 690
Bpu1102I GCTNAGC 1 cut(s) 2907
Bpu14I TTCGAA 2 cut(s) 1051, 1656
BpuEI CTTGAG 6 cut(s) 1123, 1707, 1728, 1831, 2383, 2850
BpuMI CCSGG 4 cut(s) 236, 321, 363, 364
Bsa29I ATCGAT 1 cut(s) 1815
BsaAI YACGTR 2 cut(s) 671, 2771
BsaHI GRCGYC 2 cut(s) 228, 336
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 8 cut(s) 192, 362, 363, 526, 527, 748, 904, 2676
BsaWI WCCGGW 3 cut(s) 289, 550, 1403
BsaXI ACNNNNNCTCC 4 cut(s) 377, 407, 1235, 1265
Bsc4I CCNNNNNNNGG 4 cut(s) 754, 1522, 1523, 2393
Bse118I RCCGGY 2 cut(s) 280, 1403
Bse21I CCTNAGG 1 cut(s) 1170
Bse3DI GCAATG 6 cut(s) 73, 1586, 1782, 1812, 1935, 2631
BseAI TCCGGA 2 cut(s) 289, 550
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 1 cut(s) 1815
BseDI CCNNGG 8 cut(s) 192, 362, 363, 526, 527, 748, 904, 2676
BseGI GGATG 6 cut(s) 394, 745, 893, 1180, 2015, 2557
BseLI CCNNNNNNNGG 4 cut(s) 754, 1522, 1523, 2393
BseMI GCAATG 6 cut(s) 73, 1586, 1782, 1812, 1935, 2631
BseMII CTCAG 6 cut(s) 189, 704, 1092, 1728, 3014, 3041
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 2363
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 7 cut(s) 75, 180, 183, 420, 1618, 2727, 2827
BseYI CCCAGC 3 cut(s) 1859, 2159, 2407
BsgI GTGCAG 1 cut(s) 1578
Bsh1236I CGCG 3 cut(s) 381, 2619, 2960
Bsh1285I CGRYCG 1 cut(s) 281
BshFI GGCC 6 cut(s) 280, 310, 361, 675, 688, 2963
BshNI GGYRCC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 1403
BshVI ATCGAT 1 cut(s) 1815
BsiEI CGRYCG 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 2363, 3029
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
BsiWI CGTACG 1 cut(s) 667
BslFI GGGAC 3 cut(s) 544, 2113, 2176
BslI CCNNNNNNNGG 4 cut(s) 754, 1522, 1523, 2393
BsmAI GTCTC 8 cut(s) 206, 226, 765, 1164, 1733, 1931, 2242, 2408
BsmBI CGTCTC 1 cut(s) 1931
BsmFI GGGAC 3 cut(s) 544, 2113, 2176
BsmI GAATGC 6 cut(s) 743, 970, 1219, 2059, 2374, 3018
BsnI GGCC 6 cut(s) 280, 310, 361, 675, 688, 2963
Bso31I GGTCTC 1 cut(s) 226
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 2 cut(s) 1051, 1656
Bsp1286I GDGCHC 5 cut(s) 321, 1847, 2363, 2399, 3029
Bsp13I TCCGGA 2 cut(s) 289, 550
Bsp1720I GCTNAGC 1 cut(s) 2907
Bsp19I CCATGG 2 cut(s) 748, 904
Bsp68I TCGCGA 1 cut(s) 2619
BspANI GGCC 6 cut(s) 280, 310, 361, 675, 688, 2963
BspCNI CTCAG 6 cut(s) 190, 703, 1091, 1729, 3015, 3042
BspDI ATCGAT 1 cut(s) 1815
BspEI TCCGGA 2 cut(s) 289, 550
BspFNI CGCG 3 cut(s) 381, 2619, 2960
BspHI TCATGA 1 cut(s) 2338
BspLI GGNNCC 4 cut(s) 95, 285, 485, 2694
BspMI ACCTGC 1 cut(s) 2973
BspPI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2937
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 2 cut(s) 1051, 1656
BspT107I GGYRCC 1 cut(s) 283
BspTNI GGTCTC 1 cut(s) 226
BsrBI CCGCTC 1 cut(s) 2021
BsrDI GCAATG 6 cut(s) 73, 1586, 1782, 1812, 1935, 2631
BsrFI RCCGGY 2 cut(s) 280, 1403
BssAI RCCGGY 2 cut(s) 280, 1403
BssECI CCNNGG 8 cut(s) 192, 362, 363, 526, 527, 748, 904, 2676
BssNI GRCGYC 2 cut(s) 228, 336
BssSI CACGAG 1 cut(s) 823
BssT1I CCWWGG 4 cut(s) 192, 748, 904, 2676
Bst2BI CACGAG 1 cut(s) 823
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 9 cut(s) 400, 1251, 1840, 1948, 2043, 2263, 2470, 2492, 2777
Bst6I CTCTTC 2 cut(s) 146, 1779
BstACI GRCGYC 2 cut(s) 228, 336
BstBAI YACGTR 2 cut(s) 671, 2771
BstBI TTCGAA 2 cut(s) 1051, 1656
BstDEI CTNAG 9 cut(s) 198, 690, 1078, 1170, 1737, 2612, 2907, 3023, 3050
BstDSI CCRYGG 2 cut(s) 748, 904
BstEII GGTNACC 1 cut(s) 2150
BstF5I GGATG 6 cut(s) 394, 745, 893, 1180, 2015, 2557
BstFNI CGCG 3 cut(s) 381, 2619, 2960
BstHHI GCGC 2 cut(s) 89, 381
BstMAI GTCTC 8 cut(s) 206, 226, 765, 1164, 1733, 1931, 2242, 2408
BstMCI CGRYCG 1 cut(s) 281
BstMWI GCNNNNNNNGC 5 cut(s) 84, 316, 424, 433, 3024
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 1433
BstPAI GACNNNNGTC 1 cut(s) 218
BstPI GGTNACC 1 cut(s) 2150
BstSCI CCNGG 5 cut(s) 234, 319, 361, 362, 526
BstSFI CTRYAG 1 cut(s) 2388
BstSLI GKGCMC 1 cut(s) 2363
BstUI CGCG 3 cut(s) 381, 2619, 2960
BstV1I GCAGC 7 cut(s) 75, 180, 183, 420, 1618, 2727, 2827
BstV2I GAAGAC 3 cut(s) 2131, 2200, 2675
BstX2I RGATCY 3 cut(s) 1074, 1487, 1622
BstYI RGATCY 3 cut(s) 1074, 1487, 1622
BstZI CGGCCG 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 1815
Bsu36I CCTNAGG 1 cut(s) 1170
BsuRI GGCC 6 cut(s) 280, 310, 361, 675, 688, 2963
BsuTUI ATCGAT 1 cut(s) 1815
BtgI CCRYGG 2 cut(s) 748, 904
BtgZI GCGATG 1 cut(s) 666
BtsCI GGATG 6 cut(s) 394, 745, 893, 1180, 2015, 2557
BtuMI TCGCGA 1 cut(s) 2619
BveI ACCTGC 1 cut(s) 2973
CaiI CAGNNNCTG 3 cut(s) 1472, 1919, 2261
CciI TCATGA 1 cut(s) 2338
CfoI GCGC 2 cut(s) 89, 381
Cfr10I RCCGGY 2 cut(s) 280, 1403
Cfr13I GGNCC 4 cut(s) 202, 309, 360, 484
Cfr9I CCCGGG 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 1815
CseI GACGC 2 cut(s) 344, 2966
Csp6I GTAC 9 cut(s) 208, 394, 412, 668, 1041, 2082, 2445, 2768, 2970
CspAI ACCGGT 1 cut(s) 1403
CviQI GTAC 9 cut(s) 208, 394, 412, 668, 1041, 2082, 2445, 2768, 2970
DdeI CTNAG 9 cut(s) 198, 690, 1078, 1170, 1737, 2612, 2907, 3023, 3050
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 3 cut(s) 278, 673, 2961
EagI CGGCCG 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 146, 1779
EarI CTCTTC 2 cut(s) 146, 1779
EciI GGCGGA 1 cut(s) 485
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 4 cut(s) 192, 748, 904, 2676
Eco24I GRGCYC 3 cut(s) 321, 1847, 2399
Eco31I GGTCTC 1 cut(s) 226
Eco32I GATATC 2 cut(s) 1813, 2567
Eco47I GGWCC 2 cut(s) 202, 484
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 8 cut(s) 942, 975, 1346, 1494, 1788, 1941, 2282, 2804
Eco81I CCTNAGG 1 cut(s) 1170
Eco88I CYCGRG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 2150
EcoO109I RGGNCCY 1 cut(s) 309
EcoO65I GGTNACC 1 cut(s) 2150
EcoRI GAATTC 2 cut(s) 839, 1951
EcoRII CCWGG 1 cut(s) 526
EcoRV GATATC 2 cut(s) 1813, 2567
EcoT14I CCWWGG 4 cut(s) 192, 748, 904, 2676
EcoT22I ATGCAT 3 cut(s) 745, 2376, 2557
EcoT38I GRGCYC 3 cut(s) 321, 1847, 2399
ErhI CCWWGG 4 cut(s) 192, 748, 904, 2676
Esp3I CGTCTC 1 cut(s) 1931
FaqI GGGAC 3 cut(s) 544, 2113, 2176
FauI CCCGC 2 cut(s) 106, 423
FbaI TGATCA 4 cut(s) 372, 1345, 1668, 2341
FblI GTMKAC 3 cut(s) 231, 303, 333
Fnu4HI GCNGC 9 cut(s) 64, 169, 172, 434, 437, 1632, 2716, 2816, 2961
FokI GGATG 6 cut(s) 401, 752, 900, 1187, 2002, 2564
FriOI GRGCYC 3 cut(s) 321, 1847, 2399
Fsp4HI GCNGC 9 cut(s) 64, 169, 172, 434, 437, 1632, 2716, 2816, 2961
FspBI CTAG 3 cut(s) 1071, 1340, 2792
GlaI GCGC 2 cut(s) 88, 380
GluI GCNGC 9 cut(s) 64, 169, 172, 434, 437, 1632, 2716, 2816, 2961
GsaI CCCAGC 3 cut(s) 1863, 2163, 2411
GsuI CTGGAG 3 cut(s) 1518, 1731, 2019
HaeIII GGCC 6 cut(s) 280, 310, 361, 675, 688, 2963
HapII CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
HgaI GACGC 2 cut(s) 344, 2966
HhaI GCGC 2 cut(s) 89, 381
Hin1I GRCGYC 2 cut(s) 228, 336
Hin6I GCGC 2 cut(s) 87, 379
HinP1I GCGC 2 cut(s) 87, 379
HincII GTYRAC 6 cut(s) 232, 304, 334, 602, 2473, 2646
HindII GTYRAC 6 cut(s) 232, 304, 334, 602, 2473, 2646
HpaI GTTAAC 2 cut(s) 2473, 2646
HpaII CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
HphI GGTGA 6 cut(s) 177, 335, 1418, 1703, 2692, 2744
Hpy99I CGWCG 5 cut(s) 139, 167, 233, 338, 3036
HpyAV CCTTC 8 cut(s) 190, 322, 829, 939, 1321, 1559, 1592, 1973
HpyCH4III ACNGT 9 cut(s) 400, 1251, 1840, 1948, 2043, 2263, 2470, 2492, 2777
HpyCH4IV ACGT 5 cut(s) 228, 670, 991, 2744, 2770
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 316, 424, 433, 3024
HpyF3I CTNAG 9 cut(s) 198, 690, 1078, 1170, 1737, 2612, 2907, 3023, 3050
HpySE526I ACGT 5 cut(s) 228, 670, 991, 2744, 2770
Hsp92I GRCGYC 2 cut(s) 228, 336
HspAI GCGC 2 cut(s) 87, 379
Kpn2I TCCGGA 2 cut(s) 289, 550
Ksp22I TGATCA 4 cut(s) 372, 1345, 1668, 2341
KspAI GTTAAC 2 cut(s) 2473, 2646
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 3 cut(s) 1499, 1518, 2233
Lsp1109I GCAGC 7 cut(s) 75, 180, 183, 420, 1618, 2727, 2827
LweI GCATC 9 cut(s) 340, 379, 730, 1168, 1511, 1762, 2218, 2339, 2542
MaeI CTAG 3 cut(s) 1071, 1340, 2792
MaeII ACGT 5 cut(s) 228, 670, 991, 2744, 2770
MbiI CCGCTC 1 cut(s) 2021
MflI RGATCY 3 cut(s) 1074, 1487, 1622
MhlI GDGCHC 5 cut(s) 321, 1847, 2363, 2399, 3029
MlsI TGGCCA 1 cut(s) 675
MluNI TGGCCA 1 cut(s) 675
MlyI GAGTC 9 cut(s) 107, 208, 608, 856, 1114, 1223, 1478, 1735, 2158
MmeI TCCRAC 5 cut(s) 186, 708, 748, 1510, 1659
Mox20I TGGCCA 1 cut(s) 675
Mph1103I ATGCAT 3 cut(s) 745, 2376, 2557
MroI TCCGGA 2 cut(s) 289, 550
MroXI GAANNNNTTC 2 cut(s) 839, 3018
MscI TGGCCA 1 cut(s) 675
MslI CAYNNNNRTG 2 cut(s) 348, 1224
Msp20I TGGCCA 1 cut(s) 675
MspI CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
MspR9I CCNGG 5 cut(s) 236, 321, 363, 364, 528
Mva1269I GAATGC 6 cut(s) 743, 970, 1219, 2059, 2374, 3018
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 3 cut(s) 381, 2619, 2960
MwoI GCNNNNNNNGC 5 cut(s) 84, 316, 424, 433, 3024
NciI CCSGG 4 cut(s) 236, 321, 363, 364
NcoI CCATGG 2 cut(s) 748, 904
NlaIV GGNNCC 4 cut(s) 95, 285, 485, 2694
NmeAIII GCCGAG 1 cut(s) 2437
NmuCI GTSAC 8 cut(s) 808, 1549, 1993, 2143, 2212, 2308, 2464, 2771
NruI TCGCGA 1 cut(s) 2619
NsiI ATGCAT 3 cut(s) 745, 2376, 2557
NspI RCATGY 1 cut(s) 1433
NspV TTCGAA 2 cut(s) 1051, 1656
PagI TCATGA 1 cut(s) 2338
PasI CCCWGGG 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 321
PctI GAATGC 6 cut(s) 743, 970, 1219, 2059, 2374, 3018
PdmI GAANNNNTTC 2 cut(s) 839, 3018
Pfl23II CGTACG 1 cut(s) 667
PflFI GACNNNGTC 2 cut(s) 299, 329
PflMI CCANNNNNTGG 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 1403
PkrI GCNGC 9 cut(s) 65, 170, 173, 435, 438, 1633, 2717, 2817, 2962
PleI GAGTC 9 cut(s) 107, 208, 608, 856, 1113, 1223, 1477, 1735, 2158
PpsI GAGTC 9 cut(s) 107, 208, 608, 856, 1113, 1223, 1477, 1735, 2158
Ppu21I YACGTR 2 cut(s) 671, 2771
PshAI GACNNNNGTC 1 cut(s) 218
PshBI ATTAAT 2 cut(s) 1302, 2118
Psp1406I AACGTT 1 cut(s) 2744
Psp6I CCWGG 1 cut(s) 526
PspEI GGTNACC 1 cut(s) 2150
PspFI CCCAGC 3 cut(s) 1859, 2159, 2407
PspGI CCWGG 1 cut(s) 526
PspLI CGTACG 1 cut(s) 667
PspN4I GGNNCC 4 cut(s) 95, 285, 485, 2694
PspPI GGNCC 4 cut(s) 202, 309, 360, 484
PstNI CAGNNNCTG 3 cut(s) 1472, 1919, 2261
PsuI RGATCY 3 cut(s) 1074, 1487, 1622
PsyI GACNNNGTC 2 cut(s) 299, 329
RruI TCGCGA 1 cut(s) 2619
RsaI GTAC 9 cut(s) 209, 395, 413, 669, 1042, 2083, 2446, 2769, 2971
RsaNI GTAC 9 cut(s) 208, 394, 412, 668, 1041, 2082, 2445, 2768, 2970
RseI CAYNNNNRTG 2 cut(s) 348, 1224
SalI GTCGAC 3 cut(s) 230, 302, 332
SapI GCTCTTC 1 cut(s) 146
SatI GCNGC 9 cut(s) 64, 169, 172, 434, 437, 1632, 2716, 2816, 2961
Sau96I GGNCC 4 cut(s) 202, 309, 360, 484
SchI GAGTC 9 cut(s) 107, 208, 608, 856, 1114, 1223, 1478, 1735, 2158
ScrFI CCNGG 5 cut(s) 236, 321, 363, 364, 528
SduI GDGCHC 5 cut(s) 321, 1847, 2363, 2399, 3029
SfaNI GCATC 9 cut(s) 340, 379, 730, 1168, 1511, 1762, 2218, 2339, 2542
SfcI CTRYAG 1 cut(s) 2388
SfuI TTCGAA 2 cut(s) 1051, 1656
SinI GGWCC 2 cut(s) 202, 484
SmaI CCCGGG 1 cut(s) 364
SmiMI CAYNNNNRTG 2 cut(s) 348, 1224
SmlI CTYRAG 6 cut(s) 1102, 1722, 1743, 1846, 2398, 2865
SmoI CTYRAG 6 cut(s) 1102, 1722, 1743, 1846, 2398, 2865
SspI AATATT 1 cut(s) 1417
SspMI CTAG 3 cut(s) 1071, 1340, 2792
StyD4I CCNGG 5 cut(s) 234, 319, 361, 362, 526
StyI CCWWGG 4 cut(s) 192, 748, 904, 2676
TaaI ACNGT 9 cut(s) 400, 1251, 1840, 1948, 2043, 2263, 2470, 2492, 2777
TaiI ACGT 5 cut(s) 231, 673, 994, 2747, 2773
TauI GCSGC 2 cut(s) 439, 2963
TseFI GTSAC 8 cut(s) 808, 1549, 1993, 2143, 2212, 2308, 2464, 2771
TseI GCWGC 7 cut(s) 63, 168, 171, 433, 1631, 2715, 2815
Tsp45I GTSAC 8 cut(s) 808, 1549, 1993, 2143, 2212, 2308, 2464, 2771
TspDTI ATGAA 9 cut(s) 84, 397, 560, 963, 1014, 1554, 2067, 2813, 2901
TspGWI ACGGA 6 cut(s) 266, 1038, 1130, 1594, 1955, 1976
TspMI CCCGGG 1 cut(s) 362
Tth111I GACNNNGTC 2 cut(s) 299, 329
Van91I CCANNNNNTGG 1 cut(s) 754
VneI GTGCAC 1 cut(s) 2359
VpaK11BI GGWCC 2 cut(s) 202, 484
VspI ATTAAT 2 cut(s) 1302, 2118
XapI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2526, 2844
XceI RCATGY 1 cut(s) 1433
XcmI CCANNNNNNNNNTGG 1 cut(s) 2117
XmaI CCCGGG 1 cut(s) 362
XmiI GTMKAC 3 cut(s) 231, 303, 333
XmnI GAANNNNTTC 2 cut(s) 839, 3018
XspI CTAG 3 cut(s) 1071, 1340, 2792
ZraI GACGTC 1 cut(s) 229
Zsp2I ATGCAT 3 cut(s) 745, 2376, 2557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.