Prupe.2G061500_v2.0.a1

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000527
Physical Location & Seq
Forward (+)
7806220 .. 7811613
5394 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G061500.1

Sequence Viewer

Length: 2457 bp
ATGGTGGTGAAGCGGAGCCAAACGCATTTGAGCAAGGATTCAAAGGTGGAAGTTTGCAGCAACGAAGTGGGTTACAGAGGTGCATGGTTCCCAGCCATAATCCTTGACCCTCAACCCTCAGATCTCTCACGGGAGAAGAAAAGGAAGAGCCTTGGCAACTCAAGCAACGCTCTGGTGCAGTACGAGAATCTGGTCTCTGACGATGACCCAAACAAGCCACTCACTGAGCTCGTTGACGTGCGCTACATCCGGCCAGTGCCGCCTTCTGACAATCCTGACGAGCCCTTGGAGCCAGCCGACGTCGTAGACGCCTCTTACCTTGATGCTTGGTGGGTGGGGGTTGTGATGAGGTTTGAGGATGACAAGTATACGGTGGGTTTCAAATGCCCACCTGATGTGCTTGAGTTAAGGCGCTCTGAGCTGCGGCCTCATTGGGATTTGCAGGACGGCATATGGGTTCGTGCCCGGAAGAAGAGGATGACGGGATCAATTTTCAGCCCTGGGACAGCAGTGGAAGTGAATCTCAACAAAGAACATCTATTCTGTGCGTGGTTCCCAGCAATTTATCTTGGGGAACTAGGGGTCAACTCTTTCTTGCTTCAATATAAGAGCTCTAACAACTGCGATGTTAAAGTGGTCGTAGGTGGGAAACAGATTCGGCCTCAGCCTCCAAAGTTGGCGGAAAGAGATTTTAAGTTGGAGGAAAAGGTGGATGCATTCTTTGATATGTGTTGGTGTGCCGGAGAGATTAAAAAAGTTCTTACAGGAAAAAAGTATATGGTCAGTTTGAAATTCACAGAGGAGGTGAAGCAATACAATCAGTCAGATTTGAGGCCTCACATGTACTGGACGGATGGAAGATGGGTTACTTTGATCAATGGAAAACAGTTTACTATGATCAATGAAAACTGGGTTCCTTACTTTAAGGAAGGTCACTTTAGTAGGGACCATGAAGTACGATCAAAGCTCACATGTGAGAGTTTCAGTAGTTCTGAGTTAGCTACAGCACTTGAAAGTTCAGGTGCTACCAAGGATAATAATGAAGAAAAAACACCTTGTTCGAGGATTTCGTGGAAGAATCAGTCGGAGGACTTGAGTCCTTGCATTGATAATTCACCTTATGGAAAGACCAAAAAGAAACTAAAAATTAATCAGAAACCTAACGATGATGCAACCATTTTAGGTCTGTCCAAGAAGTTAATATATGGACATTCAGAAGATTCCGTATCTTTTGCACAATTATTTCGAAGGAGAACGCCAGTTAAAACATCAAGCAAAGAAGCCCCAGTTAGAGATGGTGCGAAGACTAAGCAACAGCAGGCTGGAGGACTGGACAATCAAACAATAGTTTTTTATAAACGAAAAGTCAAGGAGGGCAGTGAAATAAAAAAGGCAGATGAAGATGTTGAAGATGAGTATGGACGAGACAACATTGAATCTTTGGGTATACTGTCAGATTTTGAACTAACAGGGGGATCTCTTGTTGACACATCATGTCTGCTGCCCATGGAGGAGGTTGAGCAGAATGAAGATGGAGTGAGCAGTGAAATAGAAGGGAAGGGAAAACTGCCTGAATATCTTATTGAACACTCAAAGGATCCAGCAACAGGTGAGAAGCAAAATGGAACTGGAGTTGTTGGTCTAGTTGAATTGACTAAGACACAAGTTGCCAATGATAATGGTTCTGAAGAAGCCAAGAGAGAGACAAAAATTGTGGATTCTGCAATGGATTACTTGACAACGGCATCTGCCCATGATCAACCTTTATCACTGTGCATCGATGAAATGCATTCTGTAAAAGCCATAGAGTGCTCAAGTAACACTACTAGGACTGCCAACCAACAAAATGAGGTTAGAGGAACACAAGTTGTGCAACAATACAGTCCACATTCGCCTTTTGTGAGAAGTTCTCCATTTTGGGAAAGTATTGAATCCATGGAAGTATTCAAAAGATTTCCGCAAAAGCCACATTTTCATCCTCTGGTGAAGTCTAAAGCAGTTTGTCGTGAAGGATCCGCCCTTGGAAATATGATAACCTTTGCCTCTTTGGTGGAGACGACTTCCAAGCTGCAAGTTGGCGATCCTAGAGACATATTTGATAGCAATTTGGAGGCACTTGTTGACTTGGAAATGTTGGGATTTGATGTAACGGCAGTACGGCATCGTCTGAAGGAGTTGGTTGAAATGAAAGTTAAGTTGGGGCAGCTTCATAACCAATTAAAAGAAGTTGACATTCAGATCAGAGAGTGTACTCTGGACAGAACCAGAAACAATGAAACCATCAGTCGGATTGATAAGAAGATTAAGGACTTGAAGGAAAAAGGGGCAACGCTGATGTCAATCAATGCGGCCAAAGGTTCTGAAATTAGCAAGCTGCAATCAGAGGCAAATGCTATCACTGAAGGCATTCAGAGCATCCATTGTGATTTTGAGAAATTAGCTGCTGCAGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

819

Amino Acids

92.13

Weight (kDa)

5.77

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1356
AatII GACGTC 1 cut(s) 303
AccI GTMKAC 3 cut(s) 306, 368, 1447
AciI CCGC 7 cut(s) 13, 260, 424, 680, 1958, 2016, 2348
AclWI GGATC 7 cut(s) 493, 1483, 1592, 1605, 2007, 2020, 2075
AcoI YGGCCR 2 cut(s) 251, 2349
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 3 cut(s) 1707, 2189, 2421
AcyI GRCGYC 2 cut(s) 300, 309
AfaI GTAC 5 cut(s) 182, 845, 957, 2157, 2251
AfiI CCNNNNNNNGG 2 cut(s) 1607, 2286
AflIII ACRYGT 2 cut(s) 840, 971
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 2180, 2212
AluBI AGCT 9 cut(s) 229, 421, 612, 967, 1001, 2068, 2206, 2374, 2441
AluI AGCT 9 cut(s) 229, 421, 612, 967, 1001, 2068, 2206, 2374, 2441
Alw21I GWGCWC 3 cut(s) 231, 614, 1814
Alw26I GTCTC 5 cut(s) 199, 1419, 1697, 2048, 2082
AlwI GGATC 7 cut(s) 493, 1483, 1592, 1605, 2007, 2020, 2075
AoxI GGCC 5 cut(s) 251, 425, 659, 833, 2349
ApeKI GCWGC 9 cut(s) 57, 421, 1501, 2068, 2203, 2374, 2441, 2444, 2447
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 1149
Asp700I GAANNNNTTC 1 cut(s) 2406
AspLEI GCGC 2 cut(s) 243, 414
AspS9I GGNCC 1 cut(s) 946
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 5 cut(s) 19, 817, 1107, 1622, 1996
AsuII TTCGAA 1 cut(s) 1246
AvaII GGWCC 1 cut(s) 946
BaeGI GKGCMC 1 cut(s) 466
BamHI GGATCC 2 cut(s) 1597, 2012
BanII GRGCYC 3 cut(s) 231, 285, 614
BarI GAAGNNNNNNTAC 2 cut(s) 850, 882
BbsI GAAGAC 1 cut(s) 1310
Bbv12I GWGCWC 3 cut(s) 231, 614, 1814
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 8 cut(s) 69, 408, 1488, 2055, 2215, 2361, 2428, 2431
BccI CCATC 5 cut(s) 848, 855, 1289, 1526, 2288
BceAI ACGGC 4 cut(s) 463, 1758, 2166, 2174
BciT130I CCWGG 1 cut(s) 501
BclI TGATCA 3 cut(s) 873, 897, 1756
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 5 cut(s) 199, 1419, 1697, 2048, 2082
BfaI CTAG 4 cut(s) 578, 1643, 1827, 2085
BfmI CTRYAG 2 cut(s) 1002, 2445
BfoI RGCGCY 1 cut(s) 415
BglII AGATCT 1 cut(s) 121
Bme1390I CCNGG 2 cut(s) 466, 501
Bme18I GGWCC 1 cut(s) 946
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 946
BmiI GGNNCC 8 cut(s) 17, 89, 291, 554, 915, 947, 1599, 2014
BmrFI CCNGG 2 cut(s) 466, 501
BmrI ACTGGG 2 cut(s) 919, 1280
BmsI GCATC 7 cut(s) 313, 703, 1159, 1754, 1785, 2170, 2424
BmuI ACTGGG 2 cut(s) 919, 1280
BoxI GACNNNNGTC 1 cut(s) 1095
BpiI GAAGAC 1 cut(s) 1310
BplI GAGNNNNNCTC 2 cut(s) 1894, 1926
BpmI CTGGAG 2 cut(s) 1344, 1650
Bpu10I CCTNAGC 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 1246
BpuEI CTTGAG 4 cut(s) 145, 422, 1114, 1798
BpuMI CCSGG 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 1779
BsaBI GATNNNNATC 1 cut(s) 2339
BsaHI GRCGYC 2 cut(s) 300, 309
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 8 cut(s) 151, 285, 499, 500, 1029, 1506, 1935, 2020
Bsc4I CCNNNNNNNGG 2 cut(s) 1607, 2286
Bse1I ACTGG 7 cut(s) 254, 851, 914, 1259, 1286, 1335, 1633
Bse3DI GCAATG 1 cut(s) 1731
Bse8I GATNNNNATC 1 cut(s) 2339
BseBI CCWGG 1 cut(s) 501
BseCI ATCGAT 1 cut(s) 1779
BseDI CCNNGG 8 cut(s) 151, 285, 499, 500, 1029, 1506, 1935, 2020
BseGI GGATG 7 cut(s) 246, 364, 483, 718, 859, 1975, 2415
BseJI GATNNNNATC 1 cut(s) 2339
BseLI CCNNNNNNNGG 2 cut(s) 1607, 2286
BseMI GCAATG 1 cut(s) 1731
BseMII CTCAG 5 cut(s) 132, 216, 408, 677, 984
BseNI ACTGG 7 cut(s) 254, 851, 914, 1259, 1286, 1335, 1633
BseRI GAGGAG 2 cut(s) 815, 1526
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 8 cut(s) 69, 408, 1488, 2055, 2215, 2361, 2428, 2431
BseYI CCCAGC 2 cut(s) 91, 556
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 5 cut(s) 253, 427, 661, 835, 2351
BshVI ATCGAT 1 cut(s) 1779
BsiHKAI GWGCWC 3 cut(s) 231, 614, 1814
BsiSI CCGG 3 cut(s) 250, 466, 741
BslFI GGGAC 2 cut(s) 517, 959
BslI CCNNNNNNNGG 2 cut(s) 1607, 2286
BsmAI GTCTC 5 cut(s) 199, 1419, 1697, 2048, 2082
BsmBI CGTCTC 1 cut(s) 2048
BsmFI GGGAC 2 cut(s) 517, 959
BsmI GAATGC 3 cut(s) 716, 1789, 2406
BsnI GGCC 5 cut(s) 253, 427, 661, 835, 2351
Bso31I GGTCTC 1 cut(s) 199
Bsp119I TTCGAA 1 cut(s) 1246
Bsp1286I GDGCHC 5 cut(s) 231, 285, 466, 614, 1814
Bsp19I CCATGG 2 cut(s) 1506, 1935
BspACI CCGC 7 cut(s) 13, 260, 424, 680, 1958, 2016, 2348
BspANI GGCC 5 cut(s) 253, 427, 661, 835, 2351
BspCNI CTCAG 5 cut(s) 131, 217, 409, 676, 985
BspDI ATCGAT 1 cut(s) 1779
BspLI GGNNCC 8 cut(s) 17, 89, 291, 554, 915, 947, 1599, 2014
BspMAI CTGCAG 1 cut(s) 2449
BspPI GGATC 7 cut(s) 493, 1483, 1592, 1605, 2007, 2020, 2075
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 1246
BspTNI GGTCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 1731
BsrI ACTGG 7 cut(s) 254, 851, 914, 1259, 1286, 1335, 1633
BssECI CCNNGG 8 cut(s) 151, 285, 499, 500, 1029, 1506, 1935, 2020
BssNAI GTATAC 2 cut(s) 369, 1448
BssNI GRCGYC 2 cut(s) 300, 309
BssT1I CCWWGG 6 cut(s) 151, 285, 1029, 1506, 1935, 2020
Bst1107I GTATAC 2 cut(s) 369, 1448
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 5 cut(s) 373, 888, 1452, 1773, 1883
Bst6I CTCTTC 2 cut(s) 140, 467
BstACI GRCGYC 2 cut(s) 300, 309
BstBI TTCGAA 1 cut(s) 1246
BstC8I GCNNGC 3 cut(s) 294, 1320, 2372
BstDEI CTNAG 7 cut(s) 118, 225, 417, 663, 993, 1308, 1656
BstDSI CCRYGG 2 cut(s) 1506, 1935
BstF5I GGATG 7 cut(s) 246, 364, 483, 718, 859, 1975, 2415
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 243, 414
BstMAI GTCTC 5 cut(s) 199, 1419, 1697, 2048, 2082
BstMWI GCNNNNNNNGC 6 cut(s) 162, 259, 289, 418, 2412, 2447
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 2 cut(s) 844, 975
BstPAI GACNNNNGTC 1 cut(s) 1095
BstSCI CCNGG 2 cut(s) 464, 499
BstSFI CTRYAG 2 cut(s) 1002, 2445
BstSLI GKGCMC 1 cut(s) 466
BstV1I GCAGC 8 cut(s) 69, 408, 1488, 2055, 2215, 2361, 2428, 2431
BstV2I GAAGAC 1 cut(s) 1310
BstX2I RGATCY 4 cut(s) 121, 1475, 1597, 2012
BstYI RGATCY 4 cut(s) 121, 1475, 1597, 2012
BstZ17I GTATAC 2 cut(s) 369, 1448
Bsu15I ATCGAT 1 cut(s) 1779
BsuRI GGCC 5 cut(s) 253, 427, 661, 835, 2351
BsuTUI ATCGAT 1 cut(s) 1779
BtgI CCRYGG 2 cut(s) 1506, 1935
BtgZI GCGATG 1 cut(s) 639
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 7 cut(s) 246, 364, 483, 718, 859, 1975, 2415
BtsI GCAGTG 3 cut(s) 516, 1384, 1549
BtsIMutI CAGTG 7 cut(s) 222, 261, 516, 1384, 1549, 1769, 2397
Cac8I GCNNGC 3 cut(s) 294, 1320, 2372
CfoI GCGC 2 cut(s) 243, 414
Cfr13I GGNCC 1 cut(s) 946
ClaI ATCGAT 1 cut(s) 1779
CseI GACGC 1 cut(s) 317
Csp6I GTAC 5 cut(s) 181, 844, 956, 2156, 2250
CspCI CAANNNNNGTGG 2 cut(s) 1695, 1730
CviAII CATG 9 cut(s) 84, 841, 950, 972, 1494, 1507, 1754, 1936, 2451
CviQI GTAC 5 cut(s) 181, 844, 956, 2156, 2250
DdeI CTNAG 7 cut(s) 118, 225, 417, 663, 993, 1308, 1656
EaeI YGGCCR 2 cut(s) 251, 2349
Eam1104I CTCTTC 2 cut(s) 140, 467
EarI CTCTTC 2 cut(s) 140, 467
EciI GGCGGA 2 cut(s) 695, 2005
Ecl136II GAGCTC 2 cut(s) 229, 612
Eco130I CCWWGG 6 cut(s) 151, 285, 1029, 1506, 1935, 2020
Eco147I AGGCCT 1 cut(s) 835
Eco24I GRGCYC 3 cut(s) 231, 285, 614
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 946
Eco53kI GAGCTC 2 cut(s) 229, 612
Eco57I CTGAAG 3 cut(s) 1707, 2189, 2421
EcoICRI GAGCTC 2 cut(s) 229, 612
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 6 cut(s) 151, 285, 1029, 1506, 1935, 2020
EcoT22I ATGCAT 2 cut(s) 718, 1791
EcoT38I GRGCYC 3 cut(s) 231, 285, 614
ErhI CCWWGG 6 cut(s) 151, 285, 1029, 1506, 1935, 2020
Esp3I CGTCTC 1 cut(s) 2048
FaeI CATG 9 cut(s) 87, 844, 953, 975, 1497, 1510, 1757, 1939, 2454
FaqI GGGAC 2 cut(s) 517, 959
FatI CATG 9 cut(s) 83, 840, 949, 971, 1493, 1506, 1753, 1935, 2450
FauNDI CATATG 1 cut(s) 452
FbaI TGATCA 3 cut(s) 873, 897, 1756
FblI GTMKAC 3 cut(s) 306, 368, 1447
FokI GGATG 7 cut(s) 233, 371, 490, 725, 866, 1962, 2402
FriOI GRGCYC 3 cut(s) 231, 285, 614
FspBI CTAG 4 cut(s) 578, 1643, 1827, 2085
GlaI GCGC 2 cut(s) 242, 413
GsaI CCCAGC 2 cut(s) 95, 560
GsuI CTGGAG 2 cut(s) 1344, 1650
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 5 cut(s) 253, 427, 661, 835, 2351
HapII CCGG 3 cut(s) 250, 466, 741
HgaI GACGC 1 cut(s) 317
HhaI GCGC 2 cut(s) 243, 414
Hin1I GRCGYC 2 cut(s) 300, 309
Hin1II CATG 9 cut(s) 87, 844, 953, 975, 1497, 1510, 1757, 1939, 2454
Hin6I GCGC 2 cut(s) 241, 412
HinP1I GCGC 2 cut(s) 241, 412
HincII GTYRAC 5 cut(s) 235, 586, 1486, 2122, 2230
HindII GTYRAC 5 cut(s) 235, 586, 1486, 2122, 2230
HpaII CCGG 3 cut(s) 250, 466, 741
HphI GGTGA 5 cut(s) 19, 817, 1107, 1622, 1996
Hpy188III TCNNGA 3 cut(s) 275, 2006, 2255
Hpy99I CGWCG 2 cut(s) 302, 305
HpyAV CCTTC 9 cut(s) 273, 923, 1242, 1547, 1552, 2003, 2164, 2308, 2396
HpyCH4III ACNGT 5 cut(s) 373, 888, 1452, 1773, 1883
HpyCH4IV ACGT 2 cut(s) 237, 300
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 259, 289, 418, 2412, 2447
HpyF3I CTNAG 7 cut(s) 118, 225, 417, 663, 993, 1308, 1656
HpySE526I ACGT 2 cut(s) 237, 300
Hsp92I GRCGYC 2 cut(s) 300, 309
Hsp92II CATG 9 cut(s) 87, 844, 953, 975, 1497, 1510, 1757, 1939, 2454
HspAI GCGC 2 cut(s) 241, 412
Ksp22I TGATCA 3 cut(s) 873, 897, 1756
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 15, 289
Lsp1109I GCAGC 8 cut(s) 69, 408, 1488, 2055, 2215, 2361, 2428, 2431
LweI GCATC 7 cut(s) 313, 703, 1159, 1754, 1785, 2170, 2424
MaeI CTAG 4 cut(s) 578, 1643, 1827, 2085
MaeII ACGT 2 cut(s) 237, 300
MaeIII GTNAC 5 cut(s) 71, 865, 932, 1817, 2146
MflI RGATCY 4 cut(s) 121, 1475, 1597, 2012
MhlI GDGCHC 5 cut(s) 231, 285, 466, 614, 1814
MlyI GAGTC 1 cut(s) 1105
MmeI TCCRAC 3 cut(s) 678, 1065, 2267
Mph1103I ATGCAT 2 cut(s) 718, 1791
MroXI GAANNNNTTC 1 cut(s) 2406
MspI CCGG 3 cut(s) 250, 466, 741
MspR9I CCNGG 2 cut(s) 466, 501
Mva1269I GAATGC 3 cut(s) 716, 1789, 2406
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 6 cut(s) 162, 259, 289, 418, 2412, 2447
NciI CCSGG 1 cut(s) 466
NcoI CCATGG 2 cut(s) 1506, 1935
NdeI CATATG 1 cut(s) 452
NlaIII CATG 9 cut(s) 87, 844, 953, 975, 1497, 1510, 1757, 1939, 2454
NlaIV GGNNCC 8 cut(s) 17, 89, 291, 554, 915, 947, 1599, 2014
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 2 cut(s) 718, 1791
NspI RCATGY 2 cut(s) 844, 975
NspV TTCGAA 1 cut(s) 1246
PasI CCCWGGG 1 cut(s) 500
PceI AGGCCT 1 cut(s) 835
PciI ACATGT 2 cut(s) 840, 971
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 1067
PctI GAATGC 3 cut(s) 716, 1789, 2406
PdmI GAANNNNTTC 1 cut(s) 2406
PfeI GAWTC 9 cut(s) 38, 187, 520, 655, 1078, 1220, 1436, 1718, 1931
PleI GAGTC 1 cut(s) 1104
PpsI GAGTC 1 cut(s) 1104
PscI ACATGT 2 cut(s) 840, 971
PshAI GACNNNNGTC 1 cut(s) 1095
PshBI ATTAAT 1 cut(s) 1149
PsiI TTATAA 1 cut(s) 1356
Psp124BI GAGCTC 2 cut(s) 231, 614
Psp6I CCWGG 1 cut(s) 499
PspFI CCCAGC 2 cut(s) 91, 556
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 8 cut(s) 17, 89, 291, 554, 915, 947, 1599, 2014
PspPI GGNCC 1 cut(s) 946
PstI CTGCAG 1 cut(s) 2449
PsuI RGATCY 4 cut(s) 121, 1475, 1597, 2012
RsaI GTAC 5 cut(s) 182, 845, 957, 2157, 2251
RsaNI GTAC 5 cut(s) 181, 844, 956, 2156, 2250
SacI GAGCTC 2 cut(s) 231, 614
SapI GCTCTTC 1 cut(s) 140
Sau96I GGNCC 1 cut(s) 946
SchI GAGTC 1 cut(s) 1105
ScrFI CCNGG 2 cut(s) 466, 501
SduI GDGCHC 5 cut(s) 231, 285, 466, 614, 1814
SfaNI GCATC 7 cut(s) 313, 703, 1159, 1754, 1785, 2170, 2424
SfcI CTRYAG 2 cut(s) 1002, 2445
SfuI TTCGAA 1 cut(s) 1246
SinI GGWCC 1 cut(s) 946
SmlI CTYRAG 4 cut(s) 160, 401, 1093, 1813
SmoI CTYRAG 4 cut(s) 160, 401, 1093, 1813
SseBI AGGCCT 1 cut(s) 835
SsiI CCGC 7 cut(s) 13, 260, 424, 680, 1958, 2016, 2348
SspMI CTAG 4 cut(s) 578, 1643, 1827, 2085
SstI GAGCTC 2 cut(s) 231, 614
StuI AGGCCT 1 cut(s) 835
StyD4I CCNGG 2 cut(s) 464, 499
StyI CCWWGG 6 cut(s) 151, 285, 1029, 1506, 1935, 2020
TaaI ACNGT 5 cut(s) 373, 888, 1452, 1773, 1883
TaiI ACGT 2 cut(s) 240, 303
TaqI TCGA 3 cut(s) 1061, 1246, 1779
TatI WGTACW 2 cut(s) 843, 2249
TauI GCSGC 3 cut(s) 262, 427, 2351
TfiI GAWTC 9 cut(s) 38, 187, 520, 655, 1078, 1220, 1436, 1718, 1931
TscAI CASTG 7 cut(s) 229, 261, 516, 1384, 1549, 1776, 2404
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 9 cut(s) 57, 421, 1501, 2068, 2203, 2374, 2441, 2444, 2447
Tsp45I GTSAC 1 cut(s) 932
TspGWI ACGGA 2 cut(s) 866, 1213
TspRI CASTG 7 cut(s) 229, 261, 516, 1384, 1549, 1776, 2404
VpaK11BI GGWCC 1 cut(s) 946
VspI ATTAAT 1 cut(s) 1149
XapI RAATTY 1 cut(s) 791
XceI RCATGY 2 cut(s) 844, 975
XmiI GTMKAC 3 cut(s) 306, 368, 1447
XmnI GAANNNNTTC 1 cut(s) 2406
XspI CTAG 4 cut(s) 578, 1643, 1827, 2085
ZraI GACGTC 1 cut(s) 301
Zsp2I ATGCAT 2 cut(s) 718, 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.