Rroxscaffold_4G00284490

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000527
Physical Location & Seq
Forward (+)
6077654 .. 6081585
3932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284490.1

Sequence Viewer

Length: 2121 bp
ATGAAGAACCCGAAGGAGGAGATCAGTTGTCTCCCATGTGGGAATTTCAGCCGCGGCGCGAAAGTCGAGGTGCAAGACGAACAAGGTGCATGGTTTCCTGCTCATGTCCTGGACCCAAATCCAGACCCAAACCCAAACCCAAACCTGTCCCCATTCTCGCTTCGGAATCGGAAGCAGAAGTTGCTGGTGGTAGAGTATGAAACTCTCGTCTCCGACGACGACCCTTCGAAGCCACTGACGGAGGAAATCGAGGTGGGTATGCTCCGACCGTGGCCGCCTGAGGAGGAGGAGGAGTGGTTTGAGGTAAACGACGTCGTTGACGCGTTTAATTTGGACGCGTGGTGGCCCGGGGTGGTGATGAGTGTTGTGGGGGACAAGTACACGGTGGGGTTTAAGAGCCCGCCGGATTTGCTGCAGCTTGGGCGTGGCGAGCTGCGGCGCCATTGGGATTGGCGCGGCGGCGAGTGGAGTCGCGCCGGGAAGGAGATGATGATGCGATTGGATTTCAGCCCTGGGGAAGATGTTGAAGTGAATCTACACAAAGAACGTGTATGGTATGCTTGGATACCGGCAATTTATGTTGGTCCATTAGGGGGCAATTCTTTCCTGGTCCGTTATAAGGGTGCGAATAATGGTGATGAAAATGGTTTTGTTAAAGCTGTATCGGACTATCAGATTCGACCTCTCCCTCCGCAGCAAGAGGAAAGAGCTTTTGAATTGTTGGCAATGGTGGATGCATACCAAGATATGTGTTGGTGGGTTGGAGACATTACAACAGTTCTTACAGGGGGAAAGGATGATGGCGATGATATCAGTCAGTTCATCATTTCAGTCTTTTCTTTTGGATTAGCATCATTATGTAATGTGATTCTGGAAGTTCAACGATGTCCCTCATTAGAATTACCCCCACATTCCTCTTGCAGAAAGTCTGAGCAACAACATGTTGGGCAACTGGATGTTGAAGTAACCACTCCCATAAAAAGAAGGGGTAGACGACGAAAATCACAAGCTGATAGCCCATTCCCTGGTGTTAGACAGGTTTCAGTCAAGAAGAGCAGTGGAATTGACAAAGCATATGAACAAAATCTTGAAGATGAGCATGTAATTAACGGAATTGAATCTTCTGGGATCGGTTCGGAGAATGAAGTAATGGGGGGATCAAAGGGTGGAAGCTCATGTCTGCTCCCTATGGAGGATAACCAACAAAATAATGAAGATGGAACAAGCAGTGGAATAAAACTGAAGAGAAAACTGCCTATGTTGGTTGTTGAACACTCAAAAGATCCAACAACAGGTTCTCGAGATTCGGTGAGAGATGGCCGAGCTGTAGCTTCTGTAGAGAAAGTTAAGCTGGCTGTGTCTGGAATTCCTGCAAAGGAACCTGATCATGATCAATCTTTATTTATAAAGGAAGGGAATTCTGTAAAAACCGTGGAGGGATCAACTGGAACTGCCAACCAACAAAATGAAGTAAGTGGAAGAAAAATTGAAACTGGAGTCAAAGGTGATGTGGAGCAAGAAGACAGTCCAAATTTGCCTTTTGTGAAAAGTATCCCATTTTGGGAAAATATTGAATCCATGGAAGTATTCAAAAGATTTCCACAGAAGCCGCATTTTCATCCTCTACTGAAAACTAAAGAGGTGTTTCGTGAGGGATCGGCTTTCGGAAAAATGTTAGCCTTTAGCTCTTTGGTAGACCGGACCTCCAAGATACGGATTGGTGATCCTAGAAACGTCATTGATAGCAACTTGGAAGCGACAGTTGAATTGGAACTGCTCGGCTTTGATGTTGATGCCATAAAGTGTCGTCTGACTGGGTTGCTAGACCTGAAAGCCAAGCTGGAGGAGCTTCAAAACCAGTCCAAAGTAGTTGAAACCAAGATCACGGCGTGTAGACAAAACACAATCAAGGACACTGAAAATATCAGTAGAATTGACAAGGAGATCAAGGACTTAGAAGCAAACATCAAGGGCTTGAAGAAAAAACGGGCCATGGTTGTGTCAATGTGTTCGGCCGAAGATTCCGAGCTTAGCAAGTTGCAGTCAGAAGCAAAAGTTATCAGTGAAGGCATTCAGAGCATCCAACATGGTTTCGACATACTAGCTGCTGCTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

706

Amino Acids

78.66

Weight (kDa)

5.17

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 19 - 93 1.3e-07 Agenet domain
Agenet PF05641 172 - 231 1.9e-06 Agenet domain
DUF724 PF05266 512 - 705 3.4e-52 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 618, 1406
AatII GACGTC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 438
AccB7I CCANNNNNTGG 1 cut(s) 1025
AccI GTMKAC 3 cut(s) 991, 1695, 1894
AccII CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
AciI CCGC 9 cut(s) 52, 54, 275, 401, 436, 456, 459, 692, 1610
AclWI GGATC 6 cut(s) 1136, 1165, 1277, 1447, 1663, 1718
AcoI YGGCCR 3 cut(s) 272, 1318, 2013
AcsI RAATTY 4 cut(s) 43, 1365, 1417, 1531
AcuI CTGAAG 1 cut(s) 1262
AcyI GRCGYC 2 cut(s) 312, 439
AdeI CACNNNGTG 1 cut(s) 1890
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 8 cut(s) 16, 593, 619, 1025, 1192, 1292, 1561, 1713
AflIII ACRYGT 4 cut(s) 321, 336, 547, 940
AjnI CCWGG 4 cut(s) 108, 511, 606, 1024
AjuI GAANNNNNNNTTGG 2 cut(s) 1279, 1311
Alw26I GTCTC 3 cut(s) 35, 214, 759
AlwI GGATC 6 cut(s) 1136, 1165, 1277, 1447, 1663, 1718
Ama87I CYCGRG 2 cut(s) 347, 1299
AoxI GGCC 5 cut(s) 272, 344, 1318, 1989, 2013
ApeKI GCWGC 6 cut(s) 412, 415, 433, 694, 2105, 2108
ApoI RAATTY 4 cut(s) 43, 1365, 1417, 1531
Asp700I GAANNNNTTC 1 cut(s) 2070
AspLEI GCGC 4 cut(s) 59, 441, 456, 476
AspS9I GGNCC 6 cut(s) 112, 345, 584, 610, 1701, 1989
AsuC2I CCSGG 3 cut(s) 348, 349, 478
AsuHPI GGTGA 5 cut(s) 367, 647, 1321, 1517, 1733
AsuII TTCGAA 1 cut(s) 227
AvaI CYCGRG 2 cut(s) 347, 1299
AvaII GGWCC 4 cut(s) 112, 584, 610, 1701
AxyI CCTNAGG 1 cut(s) 279
BanI GGYRCC 1 cut(s) 438
BanII GRGCYC 1 cut(s) 401
BarI GAAGNNNNNNTAC 2 cut(s) 519, 551
BbsI GAAGAC 1 cut(s) 1527
BbvI GCAGC 6 cut(s) 399, 420, 427, 706, 2092, 2095
BccI CCATC 3 cut(s) 794, 1211, 1310
BceAI ACGGC 1 cut(s) 1902
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 110, 513, 608, 1026
BciVI GTATCC 2 cut(s) 558, 1562
BclI TGATCA 2 cut(s) 1384, 1390
BcnI CCSGG 3 cut(s) 348, 349, 478
BcoDI GTCTC 3 cut(s) 35, 214, 759
BfaI CTAG 3 cut(s) 1728, 1823, 2102
BfmI CTRYAG 3 cut(s) 413, 1326, 1335
BfoI RGCGCY 1 cut(s) 442
BfuI GTATCC 2 cut(s) 558, 1562
BlpI GCTNAGC 1 cut(s) 2030
Bme1390I CCNGG 7 cut(s) 110, 348, 349, 478, 513, 608, 1026
Bme18I GGWCC 4 cut(s) 112, 584, 610, 1701
BmeT110I CYCGRG 2 cut(s) 347, 1299
BmgT120I GGNCC 6 cut(s) 112, 345, 584, 610, 1701, 1989
BmiI GGNNCC 3 cut(s) 114, 440, 1380
BmrFI CCNGG 7 cut(s) 110, 348, 349, 478, 513, 608, 1026
BmrI ACTGGG 1 cut(s) 1824
BmsI GCATC 5 cut(s) 483, 724, 860, 1783, 2088
BmuI ACTGGG 1 cut(s) 1824
BpiI GAAGAC 1 cut(s) 1527
BpmI CTGGAG 2 cut(s) 1515, 1862
Bpu1102I GCTNAGC 1 cut(s) 2030
Bpu14I TTCGAA 1 cut(s) 227
BpuMI CCSGG 3 cut(s) 348, 349, 478
BsaBI GATNNNNATC 2 cut(s) 850, 1389
BsaHI GRCGYC 2 cut(s) 312, 439
BsaWI WCCGGW 1 cut(s) 1698
BsaXI ACNNNNNCTCC 6 cut(s) 278, 281, 308, 311, 1688, 1718
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 593, 619, 1025, 1192, 1292, 1561, 1713
Bse118I RCCGGY 1 cut(s) 568
Bse1I ACTGG 5 cut(s) 957, 1450, 1498, 1819, 1858
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 2 cut(s) 850, 1389
BseBI CCWGG 4 cut(s) 110, 513, 608, 1026
BseGI GGATG 5 cut(s) 739, 802, 961, 1618, 2079
BseJI GATNNNNATC 2 cut(s) 850, 1389
BseLI CCNNNNNNNGG 8 cut(s) 16, 593, 619, 1025, 1192, 1292, 1561, 1713
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 270, 921
BseNI ACTGG 5 cut(s) 957, 1450, 1498, 1819, 1858
BseRI GAGGAG 6 cut(s) 32, 296, 299, 302, 305, 1859
BseX3I CGGCCG 1 cut(s) 2013
BseXI GCAGC 6 cut(s) 399, 420, 427, 706, 2092, 2095
Bsh1236I CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
Bsh1285I CGRYCG 2 cut(s) 269, 2016
BshFI GGCC 5 cut(s) 274, 346, 1320, 1991, 2015
BshNI GGYRCC 1 cut(s) 438
BsiEI CGRYCG 2 cut(s) 269, 2016
BsiHKCI CYCGRG 2 cut(s) 347, 1299
BsiSI CCGG 5 cut(s) 348, 404, 477, 569, 1699
BslFI GGGAC 3 cut(s) 133, 386, 873
BslI CCNNNNNNNGG 8 cut(s) 16, 593, 619, 1025, 1192, 1292, 1561, 1713
BsmAI GTCTC 3 cut(s) 35, 214, 759
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 3 cut(s) 133, 386, 873
BsmI GAATGC 1 cut(s) 2070
BsnI GGCC 5 cut(s) 274, 346, 1320, 1991, 2015
BsoBI CYCGRG 2 cut(s) 347, 1299
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 401
Bsp1720I GCTNAGC 1 cut(s) 2030
Bsp19I CCATGG 2 cut(s) 1578, 1992
BspACI CCGC 9 cut(s) 52, 54, 275, 401, 436, 456, 459, 692, 1610
BspANI GGCC 5 cut(s) 274, 346, 1320, 1991, 2015
BspCNI CTCAG 2 cut(s) 271, 922
BspFNI CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
BspHI TCATGA 1 cut(s) 1387
BspLI GGNNCC 3 cut(s) 114, 440, 1380
BspMAI CTGCAG 1 cut(s) 417
BspPI GGATC 6 cut(s) 1136, 1165, 1277, 1447, 1663, 1718
BspQI GCTCTTC 1 cut(s) 1046
BspT104I TTCGAA 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 438
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 1 cut(s) 568
BsrI ACTGG 5 cut(s) 957, 1450, 1498, 1819, 1858
BssAI RCCGGY 1 cut(s) 568
BssNI GRCGYC 2 cut(s) 312, 439
BssT1I CCWWGG 2 cut(s) 1578, 1992
Bst2UI CCWGG 4 cut(s) 110, 513, 608, 1026
Bst4CI ACNGT 6 cut(s) 270, 385, 778, 1432, 1526, 1762
Bst6I CTCTTC 2 cut(s) 1046, 1238
BstACI GRCGYC 2 cut(s) 312, 439
BstAPI GCANNNNNTGC 1 cut(s) 181
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 401, 431, 1353
BstDEI CTNAG 4 cut(s) 279, 930, 1954, 2030
BstDSI CCRYGG 5 cut(s) 52, 269, 1431, 1578, 1992
BstF5I GGATG 5 cut(s) 739, 802, 961, 1618, 2079
BstFNI CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 4 cut(s) 59, 441, 456, 476
BstMAI GTCTC 3 cut(s) 35, 214, 759
BstMCI CGRYCG 2 cut(s) 269, 2016
BstMWI GCNNNNNNNGC 6 cut(s) 181, 409, 421, 430, 1846, 2076
BstNI CCWGG 4 cut(s) 110, 513, 608, 1026
BstNSI RCATGY 2 cut(s) 944, 1103
BstSCI CCNGG 7 cut(s) 108, 346, 347, 476, 511, 606, 1024
BstSFI CTRYAG 3 cut(s) 413, 1326, 1335
BstUI CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
BstV1I GCAGC 6 cut(s) 399, 420, 427, 706, 2092, 2095
BstV2I GAAGAC 1 cut(s) 1527
BstX2I RGATCY 1 cut(s) 1282
BstYI RGATCY 1 cut(s) 1282
BstZI CGGCCG 1 cut(s) 2013
Bsu36I CCTNAGG 1 cut(s) 279
BsuI GTATCC 2 cut(s) 558, 1562
BsuRI GGCC 5 cut(s) 274, 346, 1320, 1991, 2015
BtgI CCRYGG 5 cut(s) 52, 269, 1431, 1578, 1992
BtgZI GCGATG 1 cut(s) 819
BtsCI GGATG 5 cut(s) 739, 802, 961, 1618, 2079
BtsI GCAGTG 2 cut(s) 1063, 1234
BtsIMutI CAGTG 5 cut(s) 233, 1063, 1234, 1914, 2068
Cac8I GCNNGC 3 cut(s) 401, 431, 1353
CciI TCATGA 1 cut(s) 1387
CfoI GCGC 4 cut(s) 59, 441, 456, 476
Cfr10I RCCGGY 1 cut(s) 568
Cfr13I GGNCC 6 cut(s) 112, 345, 584, 610, 1701, 1989
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 347
CseI GACGC 2 cut(s) 329, 344
Csp6I GTAC 1 cut(s) 379
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 4 cut(s) 279, 930, 1954, 2030
DinI GGCGCC 1 cut(s) 440
DraIII CACNNNGTG 1 cut(s) 1890
EaeI YGGCCR 3 cut(s) 272, 1318, 2013
EagI CGGCCG 1 cut(s) 2013
Eam1104I CTCTTC 2 cut(s) 1046, 1238
EarI CTCTTC 2 cut(s) 1046, 1238
EclXI CGGCCG 1 cut(s) 2013
Eco130I CCWWGG 2 cut(s) 1578, 1992
Eco24I GRGCYC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 811
Eco47I GGWCC 4 cut(s) 112, 584, 610, 1701
Eco52I CGGCCG 1 cut(s) 2013
Eco57I CTGAAG 1 cut(s) 1262
Eco81I CCTNAGG 1 cut(s) 279
Eco88I CYCGRG 2 cut(s) 347, 1299
EcoRI GAATTC 2 cut(s) 1365, 1417
EcoRII CCWGG 4 cut(s) 108, 511, 606, 1024
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 2 cut(s) 1578, 1992
EcoT22I ATGCAT 1 cut(s) 739
EcoT38I GRGCYC 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 440
EheI GGCGCC 1 cut(s) 440
ErhI CCWWGG 2 cut(s) 1578, 1992
Esp3I CGTCTC 1 cut(s) 214
FaqI GGGAC 3 cut(s) 133, 386, 873
FauI CCCGC 1 cut(s) 408
FauNDI CATATG 1 cut(s) 1075
FbaI TGATCA 2 cut(s) 1384, 1390
FblI GTMKAC 3 cut(s) 991, 1695, 1894
FokI GGATG 5 cut(s) 746, 809, 968, 1605, 2066
FriOI GRGCYC 1 cut(s) 401
FspBI CTAG 3 cut(s) 1728, 1823, 2102
GlaI GCGC 4 cut(s) 58, 440, 455, 475
GsuI CTGGAG 2 cut(s) 1515, 1862
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 5 cut(s) 274, 346, 1320, 1991, 2015
HapII CCGG 5 cut(s) 348, 404, 477, 569, 1699
HgaI GACGC 2 cut(s) 329, 344
HhaI GCGC 4 cut(s) 59, 441, 456, 476
Hin1I GRCGYC 2 cut(s) 312, 439
Hin6I GCGC 4 cut(s) 57, 439, 454, 474
HinP1I GCGC 4 cut(s) 57, 439, 454, 474
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HpaII CCGG 5 cut(s) 348, 404, 477, 569, 1699
HphI GGTGA 5 cut(s) 367, 647, 1321, 1517, 1733
Hpy166II GTNNAC 6 cut(s) 307, 319, 381, 992, 1696, 1895
Hpy188III TCNNGA 9 cut(s) 122, 872, 1048, 1088, 1299, 1301, 1362, 1388, 1649
Hpy8I GTNNAC 6 cut(s) 307, 319, 381, 992, 1696, 1895
Hpy99I CGWCG 5 cut(s) 218, 221, 314, 317, 999
HpyAV CCTTC 6 cut(s) 7, 234, 475, 978, 1406, 2060
HpyCH4III ACNGT 6 cut(s) 270, 385, 778, 1432, 1526, 1762
HpyCH4IV ACGT 3 cut(s) 312, 547, 1734
HpyCH4V TGCA 7 cut(s) 73, 89, 415, 737, 921, 1373, 2041
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 409, 421, 430, 1846, 2076
HpyF3I CTNAG 4 cut(s) 279, 930, 1954, 2030
HpySE526I ACGT 3 cut(s) 312, 547, 1734
Hsp92I GRCGYC 2 cut(s) 312, 439
HspAI GCGC 4 cut(s) 57, 439, 454, 474
KasI GGCGCC 1 cut(s) 438
Ksp22I TGATCA 2 cut(s) 1384, 1390
KspI CCGCGG 1 cut(s) 55
LguI GCTCTTC 1 cut(s) 1046
LmnI GCTCC 5 cut(s) 267, 1188, 1513, 1846, 2116
Lsp1109I GCAGC 6 cut(s) 399, 420, 427, 706, 2092, 2095
LweI GCATC 5 cut(s) 483, 724, 860, 1783, 2088
MaeI CTAG 3 cut(s) 1728, 1823, 2102
MaeII ACGT 3 cut(s) 312, 547, 1734
MaeIII GTNAC 1 cut(s) 964
MflI RGATCY 1 cut(s) 1282
MhlI GDGCHC 1 cut(s) 401
MluI ACGCGT 2 cut(s) 321, 336
Mly113I GGCGCC 1 cut(s) 439
MlyI GAGTC 2 cut(s) 478, 1506
MmeI TCCRAC 5 cut(s) 237, 289, 742, 1310, 2107
Mph1103I ATGCAT 1 cut(s) 739
MroXI GAANNNNTTC 1 cut(s) 2070
MseI TTAA 5 cut(s) 327, 393, 654, 1107, 1347
MslI CAYNNNNRTG 2 cut(s) 856, 1997
MspA1I CMGCKG 1 cut(s) 54
MspI CCGG 5 cut(s) 348, 404, 477, 569, 1699
MspR9I CCNGG 7 cut(s) 110, 348, 349, 478, 513, 608, 1026
Mva1269I GAATGC 1 cut(s) 2070
MvaI CCWGG 4 cut(s) 110, 513, 608, 1026
MvnI CGCG 6 cut(s) 54, 59, 323, 338, 456, 474
MwoI GCNNNNNNNGC 6 cut(s) 181, 409, 421, 430, 1846, 2076
NarI GGCGCC 1 cut(s) 439
NciI CCSGG 3 cut(s) 348, 349, 478
NcoI CCATGG 2 cut(s) 1578, 1992
NdeI CATATG 1 cut(s) 1075
NlaIV GGNNCC 3 cut(s) 114, 440, 1380
NmeAIII GCCGAG 2 cut(s) 1346, 1758
NsiI ATGCAT 1 cut(s) 739
NspI RCATGY 2 cut(s) 944, 1103
NspV TTCGAA 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 1299
PagI TCATGA 1 cut(s) 1387
PasI CCCWGGG 1 cut(s) 512
PciI ACATGT 1 cut(s) 940
PciSI GCTCTTC 1 cut(s) 1046
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PctI GAATGC 1 cut(s) 2070
PdmI GAANNNNTTC 1 cut(s) 2070
PfeI GAWTC 8 cut(s) 166, 532, 676, 868, 1118, 1304, 1574, 2021
PflMI CCANNNNNTGG 1 cut(s) 1025
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 2 cut(s) 477, 1505
PluTI GGCGCC 1 cut(s) 442
PpsI GAGTC 2 cut(s) 477, 1505
PscI ACATGT 1 cut(s) 940
PsiI TTATAA 2 cut(s) 618, 1406
Psp6I CCWGG 4 cut(s) 108, 511, 606, 1024
PspGI CCWGG 4 cut(s) 108, 511, 606, 1024
PspN4I GGNNCC 3 cut(s) 114, 440, 1380
PspPI GGNCC 6 cut(s) 112, 345, 584, 610, 1701, 1989
PstI CTGCAG 1 cut(s) 417
PsuI RGATCY 1 cut(s) 1282
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 2 cut(s) 856, 1997
SacII CCGCGG 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 1046
SaqAI TTAA 5 cut(s) 327, 393, 654, 1107, 1347
Sau96I GGNCC 6 cut(s) 112, 345, 584, 610, 1701, 1989
SchI GAGTC 2 cut(s) 478, 1506
ScrFI CCNGG 7 cut(s) 110, 348, 349, 478, 513, 608, 1026
SduI GDGCHC 1 cut(s) 401
SfaNI GCATC 5 cut(s) 483, 724, 860, 1783, 2088
SfcI CTRYAG 3 cut(s) 413, 1326, 1335
SfoI GGCGCC 1 cut(s) 440
Sfr274I CTCGAG 1 cut(s) 1299
Sfr303I CCGCGG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 227
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 4 cut(s) 112, 584, 610, 1701
SlaI CTCGAG 1 cut(s) 1299
SmaI CCCGGG 1 cut(s) 349
SmiMI CAYNNNNRTG 2 cut(s) 856, 1997
SmlI CTYRAG 1 cut(s) 1299
SmoI CTYRAG 1 cut(s) 1299
SsiI CCGC 9 cut(s) 52, 54, 275, 401, 436, 456, 459, 692, 1610
SspDI GGCGCC 1 cut(s) 438
SspI AATATT 1 cut(s) 1570
SspMI CTAG 3 cut(s) 1728, 1823, 2102
StyD4I CCNGG 7 cut(s) 108, 346, 347, 476, 511, 606, 1024
StyI CCWWGG 2 cut(s) 1578, 1992
TaaI ACNGT 6 cut(s) 270, 385, 778, 1432, 1526, 1762
TaiI ACGT 3 cut(s) 315, 550, 1737
TaqI TCGA 6 cut(s) 66, 227, 249, 679, 1300, 2094
TatI WGTACW 1 cut(s) 378
TauI GCSGC 7 cut(s) 54, 57, 277, 439, 459, 462, 1612
TfiI GAWTC 8 cut(s) 166, 532, 676, 868, 1118, 1304, 1574, 2021
Tru1I TTAA 5 cut(s) 327, 393, 654, 1107, 1347
Tru9I TTAA 5 cut(s) 327, 393, 654, 1107, 1347
TscAI CASTG 5 cut(s) 240, 1063, 1234, 1921, 2068
TseI GCWGC 6 cut(s) 412, 415, 433, 694, 2105, 2108
TspDTI ATGAA 9 cut(s) 17, 213, 654, 811, 1092, 1158, 1227, 1482, 1607
TspGWI ACGGA 4 cut(s) 254, 602, 1125, 1729
TspMI CCCGGG 1 cut(s) 347
TspRI CASTG 5 cut(s) 240, 1063, 1234, 1921, 2068
Van91I CCANNNNNTGG 1 cut(s) 1025
VpaK11BI GGWCC 4 cut(s) 112, 584, 610, 1701
XapI RAATTY 4 cut(s) 43, 1365, 1417, 1531
XceI RCATGY 2 cut(s) 944, 1103
XhoI CTCGAG 1 cut(s) 1299
XmaI CCCGGG 1 cut(s) 347
XmiI GTMKAC 3 cut(s) 991, 1695, 1894
XmnI GAANNNNTTC 1 cut(s) 2070
XspI CTAG 3 cut(s) 1728, 1823, 2102
ZraI GACGTC 1 cut(s) 313
Zsp2I ATGCAT 1 cut(s) 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.