Prupe.2G269000_v2.0.a1

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000527
Physical Location & Seq
Reverse (-)
27391653 .. 27398025
6373 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G269000.3

Sequence Viewer

Length: 2640 bp
ATGGTGGTGAAGCGGAGTGAAGCGCATTTGAGCAAGGATTCAAAGGTGGAAGTTTGCAGCAACGAAGAGGGTTATAGAGGTGCATGGTTCCCTGCCATAATCCTTGACCCACAACCCTCAGATCTCTCAGCGAAGAAGAAAAGGAAGAGCCTTGGCAACTCAAGCAAGGCTCTGGTGCAGTACGAGACTCTGGTCTTCGACGATGACCCAAACAAGCCACTCACTGAGTTCGTTGACGTGTGCTCCATCCGGCCCGTGCCTCCTCCTGACAATCCTGACCAGCCCTTCGAGCCCGCAGACGTCGTAGACTCCTTTTACCAAGAAGCTTGGTGGGTGGGGGTTGTGATGAGGTTTGAGGATGACAAGTATACGGTGGGTTTCAAAAACCCACCTGATCTGCTTGAGTTAAGGCGCTCTGAGCTGCGGCCTCATTGGGATTTTCTGGACGGCGTATGGATTCGAGCCCGGAAGGAGAGGATAGAGGAGACAATTTTCAGCCATGGGACAGCAGTGGAAATAAATCTCAACGAAGACAATCTATTGTATGCTTGGTTCCCAGCAATATATCTTGGTGAACTAGGGGTTAACTCTTTCTTGCTTCAATATAAGAGCTCAAACAACCGTGATGTTAAAGTGGTTGTAAATGGCCATCAGATTCGGCCTCATCCTCCAAAGTCTGGCAAAAGAGATTTTAACTTGATGGAAATGGTGGATGCATTCTATGATATGGGTTGGTGGGTCGGAGAGATTACACAAATTCTTACAGAAGAAAAGTATATGGTCCGTTCAAAATTCACAAAGCAGGTAAAGGAATGCAGTCCTTCAGAATTGAGGCCTCATGTGGAATGGACGGAGAGAGGATGGATTACAAAGATCAATGGAACATGGGTTGATTGCTATAAGGAAGCTCACTTTAGTGTGGATTATGAAGTACAATCAAAGCGTGCATGCGAGAGTTTCAGAAGCTCCGAGGTCGCTAAAGCACTTGAAAGTTCAGGTGCTACCAAGGATAATAATGAAGAAAAAACAGCTTGTTCAAGGATTTCATGGAAGAATCAGTTGGAGCACTTGAGTCCTTGCATTGATAAATCACCATATGGAAAGACCAAAAAGAAACTAAAAATTAATCAGAAACCCAATGAGGATGCAACAATTCTATGTCTTTCCAAGAATCCAGAAGACTTCTTATCTTTTACACCATCATTTTCAAGGAGAACGCCAGCTAAAACATCAAAAAGAGAAGCCCCAGTTAGAAAGGGTGCGAAGACTGAGCAACAACAGGTTGGAGAACCGGACAATCAAGAAATCATTTCTTATAAACATAAAGTCAAGAAGGGCAGTGAAATCGAAAATGCTGATGAAGAAGATGTTGAAGATGAGTATGGACTGGACAACATTGAATCTTCTGGTATAAGGTCAGATTCTGAGCTAACAGGGGGATCTCATGCTGGAACATCATGTCTGCTCCCCGTAGAGGAGGTTGAATGGAATGAAGATGGGGTGAGCAGTGAAATAAAAGGGAAGGGAAAACAGCCTGAATTTCTTGTTGAACACCCAAAAGATCCAGCAACAGATCATACGAATGATATATTTGAATTGCCTGTAACAACAATGTGGGATTTGACAGGCGAGAAGCTCAATGGAACTGGAGTTGTTGCTCAAGTTGAATTGACTAAGACTCAAGTTGTCAATGATAATGGTTTTGAAGATGCAAGGGTGGAAGCAGAAGCTGAAGATACTGCAATGGATATTGAGAAAATAATAGAGGGCTTAAGTAATCCTGCAGGGTTTTCCAGCAAACAAAATGAGGTCAGAGGAAGACAGGTTGAAACTGGTGTAACAGGTTCTGAAGAAACAATGAGAGAGACGGAAACTCTGGATTCCGCAATGGATTACTTGACAAAAGAAGTTCAGGTGACTGTGACTGGAGTATCTGCAACAGCGTCTGCCCATGATCAACCTTTATCACTGTATGTAGATGAAGTGCATTCTATAAAAGCCAAAGAGTGCTCAAGTAACCCTGCTGGGACTGCCAAGCAACAAAATGAGGTTGAGCAACAAGACAGTCCACATTCACCTTTTGTGAGAAGTTCTCCATTTTGGAAAAGTATTGAATCCATGGAAGTATTCAAAAGATTTCCGCAAAGGCCACATTTTCTTCCTCTGATGAAGTGTAAAGCGGTTTGTCGCGAAGGATCCGCTCTTGGAAATATGATAACCTTTGCCTCTTTGGTGGAGAAGACTTCCAAGTTGCAAGTTGGCGATCCTAGAGAGTTATTTGATAGCAATTTGGAGGCACTAGTTGACTTGGAAATGTTGGGATTTGATGTCACGGCAGTACGGCATCGACTGAAGGAATTGATTGAAATGAAAGTTAAGTTGGGTCAGCTTGAGAACCAATCAAAAGAAGTTGACATTCAGATCACGGAGTGTACTTTTGACAGAACCAGAAACAATGAAACAATCAGTCAGATTGATAAGGAGATCAAGGACTTGAAGGAAAAACGGGGGACCCTGATGTCAATCGATGTGGCCAAAGGTTCTGAAATTAGCAAGCTGCAATCAGAAGCAAATGCTATCACTGAAGGCATTCAGAGCATCCATCGTGATTTTGAGAAATTAGCTGCTGCAGCGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

880

Amino Acids

99.2

Weight (kDa)

5.09

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1317
AasI GACNNNNNNGTC 1 cut(s) 2518
AatII GACGTC 1 cut(s) 303
Acc36I ACCTGC 1 cut(s) 793
AccB7I CCANNNNNTGG 1 cut(s) 677
AccBSI CCGCTC 1 cut(s) 2201
AccI GTMKAC 2 cut(s) 306, 368
AccII CGCG 1 cut(s) 2190
AciI CCGC 7 cut(s) 13, 294, 424, 1884, 2141, 2180, 2199
AclWI GGATC 5 cut(s) 1447, 1556, 2190, 2203, 2258
AcoI YGGCCR 2 cut(s) 646, 2532
AcsI RAATTY 3 cut(s) 756, 791, 1538
AcuI CTGAAG 5 cut(s) 807, 1752, 1869, 2372, 2604
AcyI GRCGYC 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 2430
AfaI GTAC 4 cut(s) 182, 933, 2340, 2434
AfiI CCNNNNNNNGG 2 cut(s) 677, 1474
AflII CTTAAG 1 cut(s) 1771
AflIII ACRYGT 1 cut(s) 237
AhlI ACTAGT 1 cut(s) 2299
AjiI CACGTC 1 cut(s) 238
AjuI GAANNNNNNNTTGG 4 cut(s) 1043, 1075, 2363, 2395
AleI CACNNNNGTG 1 cut(s) 915
Alw21I GWGCWC 4 cut(s) 245, 614, 1068, 2012
Alw26I GTCTC 3 cut(s) 179, 479, 1859
AlwI GGATC 5 cut(s) 1447, 1556, 2190, 2203, 2258
AlwNI CAGNNNCTG 4 cut(s) 1424, 1730, 1847, 1946
AoxI GGCC 7 cut(s) 251, 425, 646, 659, 833, 2147, 2532
ApeKI GCWGC 6 cut(s) 57, 421, 2557, 2624, 2627, 2630
ApoI RAATTY 3 cut(s) 756, 791, 1538
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 1125
Asp700I GAANNNNTTC 1 cut(s) 2589
AspLEI GCGC 2 cut(s) 25, 414
AspS9I GGNCC 3 cut(s) 252, 781, 2511
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 6 cut(s) 19, 584, 1083, 1513, 1927, 2067
AvaII GGWCC 2 cut(s) 781, 2511
BaeI ACNNNNGTAYC 2 cut(s) 1914, 1947
BalI TGGCCA 2 cut(s) 648, 2534
BamHI GGATCC 1 cut(s) 2195
BanII GRGCYC 3 cut(s) 294, 466, 614
BbsI GAAGAC 6 cut(s) 187, 537, 1185, 1271, 1825, 2246
Bbv12I GWGCWC 4 cut(s) 245, 614, 1068, 2012
BbvI GCAGC 5 cut(s) 69, 408, 2544, 2611, 2614
BccI CCATC 7 cut(s) 254, 657, 694, 855, 1207, 1490, 2610
BceAI ACGGC 3 cut(s) 463, 2349, 2357
BcgI CGANNNNNNTGC 2 cut(s) 1327, 1361
BclI TGATCA 1 cut(s) 1954
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 179, 479, 1859
BcuI ACTAGT 1 cut(s) 2299
BfaI CTAG 3 cut(s) 578, 2268, 2300
BfmI CTRYAG 2 cut(s) 1782, 2628
BfoI RGCGCY 1 cut(s) 415
BfrI CTTAAG 1 cut(s) 1771
BfuAI ACCTGC 1 cut(s) 793
BglII AGATCT 1 cut(s) 121
BisI GCNGC 7 cut(s) 58, 422, 425, 2558, 2625, 2628, 2631
BlsI GCNGC 7 cut(s) 59, 423, 426, 2559, 2626, 2629, 2632
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 2 cut(s) 781, 2511
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 3 cut(s) 252, 781, 2511
BmiI GGNNCC 5 cut(s) 89, 554, 2197, 2512, 2513
BmrFI CCNGG 1 cut(s) 466
BmrI ACTGGG 1 cut(s) 1241
BmsI GCATC 5 cut(s) 703, 1135, 1699, 2353, 2607
BmuI ACTGGG 1 cut(s) 1241
BoxI GACNNNNGTC 1 cut(s) 191
BpiI GAAGAC 6 cut(s) 187, 537, 1185, 1271, 1825, 2246
BplI GAGNNNNNCTC 2 cut(s) 2077, 2109
BpmI CTGGAG 2 cut(s) 1668, 1947
BpuEI CTTGAG 7 cut(s) 145, 422, 1090, 1644, 1665, 1996, 2411
BpuMI CCSGG 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 2526
BsaBI GATNNNNATC 1 cut(s) 2522
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 5 cut(s) 151, 499, 969, 1005, 2118
BsaWI WCCGGW 1 cut(s) 1291
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 677, 1474
Bse1I ACTGG 5 cut(s) 1247, 1392, 1651, 1837, 1930
Bse3DI GCAATG 2 cut(s) 1749, 1893
Bse8I GATNNNNATC 1 cut(s) 2522
BseCI ATCGAT 1 cut(s) 2526
BseDI CCNNGG 5 cut(s) 151, 499, 969, 1005, 2118
BseGI GGATG 7 cut(s) 246, 364, 664, 718, 866, 1150, 2598
BseJI GATNNNNATC 1 cut(s) 2522
BseLI CCNNNNNNNGG 2 cut(s) 677, 1474
BseMI GCAATG 2 cut(s) 1749, 1893
BseMII CTCAG 6 cut(s) 132, 141, 216, 408, 1260, 1416
BseNI ACTGG 5 cut(s) 1247, 1392, 1651, 1837, 1930
BseRI GAGGAG 3 cut(s) 252, 497, 1490
BseXI GCAGC 5 cut(s) 69, 408, 2544, 2611, 2614
BseYI CCCAGC 2 cut(s) 556, 2024
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 2190
BshFI GGCC 7 cut(s) 253, 427, 648, 661, 835, 2149, 2534
BshVI ATCGAT 1 cut(s) 2526
BsiHKAI GWGCWC 4 cut(s) 245, 614, 1068, 2012
BsiSI CCGG 3 cut(s) 250, 466, 1292
BslFI GGGAC 3 cut(s) 517, 2041, 2524
BslI CCNNNNNNNGG 2 cut(s) 677, 1474
BsmAI GTCTC 3 cut(s) 179, 479, 1859
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 3 cut(s) 517, 2041, 2524
BsmI GAATGC 4 cut(s) 716, 818, 1987, 2589
BsnI GGCC 7 cut(s) 253, 427, 648, 661, 835, 2149, 2534
Bsp1286I GDGCHC 6 cut(s) 245, 294, 466, 614, 1068, 2012
Bsp19I CCATGG 2 cut(s) 499, 2118
Bsp68I TCGCGA 1 cut(s) 2190
BspACI CCGC 7 cut(s) 13, 294, 424, 1884, 2141, 2180, 2199
BspANI GGCC 7 cut(s) 253, 427, 648, 661, 835, 2149, 2534
BspCNI CTCAG 6 cut(s) 131, 140, 217, 409, 1261, 1417
BspDI ATCGAT 1 cut(s) 2526
BspFNI CGCG 1 cut(s) 2190
BspLI GGNNCC 5 cut(s) 89, 554, 2197, 2512, 2513
BspMAI CTGCAG 2 cut(s) 1786, 2632
BspMI ACCTGC 1 cut(s) 793
BspPI GGATC 5 cut(s) 1447, 1556, 2190, 2203, 2258
BspQI GCTCTTC 1 cut(s) 140
BspTI CTTAAG 1 cut(s) 1771
BsrBI CCGCTC 1 cut(s) 2201
BsrDI GCAATG 2 cut(s) 1749, 1893
BsrI ACTGG 5 cut(s) 1247, 1392, 1651, 1837, 1930
BssECI CCNNGG 5 cut(s) 151, 499, 969, 1005, 2118
BssNAI GTATAC 1 cut(s) 369
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 4 cut(s) 151, 499, 1005, 2118
Bst1107I GTATAC 1 cut(s) 369
Bst4CI ACNGT 5 cut(s) 373, 623, 1921, 1971, 2066
Bst6I CTCTTC 2 cut(s) 60, 140
BstACI GRCGYC 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 1771
BstC8I GCNNGC 5 cut(s) 294, 945, 949, 1221, 2555
BstDEI CTNAG 7 cut(s) 118, 127, 225, 417, 1269, 1425, 1674
BstDSI CCRYGG 2 cut(s) 499, 2118
BstF5I GGATG 7 cut(s) 246, 364, 664, 718, 866, 1150, 2598
BstFNI CGCG 1 cut(s) 2190
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 25, 414
BstMAI GTCTC 3 cut(s) 179, 479, 1859
BstMWI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 2030, 2595, 2630
BstNSI RCATGY 1 cut(s) 951
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 464
BstSFI CTRYAG 2 cut(s) 1782, 2628
BstUI CGCG 1 cut(s) 2190
BstV1I GCAGC 5 cut(s) 69, 408, 2544, 2611, 2614
BstV2I GAAGAC 6 cut(s) 187, 537, 1185, 1271, 1825, 2246
BstX2I RGATCY 4 cut(s) 121, 1439, 1561, 2195
BstYI RGATCY 4 cut(s) 121, 1439, 1561, 2195
BstZ17I GTATAC 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 2526
BsuRI GGCC 7 cut(s) 253, 427, 648, 661, 835, 2149, 2534
BsuTUI ATCGAT 1 cut(s) 2526
BtgI CCRYGG 2 cut(s) 499, 2118
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 7 cut(s) 246, 364, 664, 718, 866, 1150, 2598
BtsI GCAGTG 3 cut(s) 516, 1345, 1513
BtsIMutI CAGTG 6 cut(s) 222, 516, 1345, 1513, 1967, 2580
BtuMI TCGCGA 1 cut(s) 2190
BveI ACCTGC 1 cut(s) 793
Cac8I GCNNGC 5 cut(s) 294, 945, 949, 1221, 2555
CaiI CAGNNNCTG 4 cut(s) 1424, 1730, 1847, 1946
CfoI GCGC 2 cut(s) 25, 414
Cfr13I GGNCC 3 cut(s) 252, 781, 2511
ClaI ATCGAT 1 cut(s) 2526
CseI GACGC 1 cut(s) 1932
Csp6I GTAC 4 cut(s) 181, 932, 2339, 2433
CspCI CAANNNNNGTGG 2 cut(s) 2511, 2546
CviQI GTAC 4 cut(s) 181, 932, 2339, 2433
DdeI CTNAG 7 cut(s) 118, 127, 225, 417, 1269, 1425, 1674
DraIII CACNNNGTG 1 cut(s) 2430
DrdI GACNNNNNNGTC 1 cut(s) 2518
DseDI GACNNNNNNGTC 1 cut(s) 2518
EaeI YGGCCR 2 cut(s) 646, 2532
Eam1104I CTCTTC 2 cut(s) 60, 140
EarI CTCTTC 2 cut(s) 60, 140
Ecl136II GAGCTC 1 cut(s) 612
Eco130I CCWWGG 4 cut(s) 151, 499, 1005, 2118
Eco147I AGGCCT 1 cut(s) 835
Eco24I GRGCYC 3 cut(s) 294, 466, 614
Eco47I GGWCC 2 cut(s) 781, 2511
Eco53kI GAGCTC 1 cut(s) 612
Eco57I CTGAAG 5 cut(s) 807, 1752, 1869, 2372, 2604
EcoICRI GAGCTC 1 cut(s) 612
EcoO109I RGGNCCY 1 cut(s) 2511
EcoT14I CCWWGG 4 cut(s) 151, 499, 1005, 2118
EcoT22I ATGCAT 1 cut(s) 718
EcoT38I GRGCYC 3 cut(s) 294, 466, 614
ErhI CCWWGG 4 cut(s) 151, 499, 1005, 2118
Esp3I CGTCTC 1 cut(s) 1859
FaqI GGGAC 3 cut(s) 517, 2041, 2524
FauI CCCGC 1 cut(s) 301
FauNDI CATATG 1 cut(s) 1096
FbaI TGATCA 1 cut(s) 1954
FblI GTMKAC 2 cut(s) 306, 368
Fnu4HI GCNGC 7 cut(s) 58, 422, 425, 2558, 2625, 2628, 2631
FokI GGATG 7 cut(s) 233, 371, 651, 725, 873, 1157, 2585
FriOI GRGCYC 3 cut(s) 294, 466, 614
Fsp4HI GCNGC 7 cut(s) 58, 422, 425, 2558, 2625, 2628, 2631
FspBI CTAG 3 cut(s) 578, 2268, 2300
GlaI GCGC 2 cut(s) 24, 413
GluI GCNGC 7 cut(s) 58, 422, 425, 2558, 2625, 2628, 2631
GsaI CCCAGC 2 cut(s) 560, 2028
GsuI CTGGAG 2 cut(s) 1668, 1947
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 7 cut(s) 253, 427, 648, 661, 835, 2149, 2534
HapII CCGG 3 cut(s) 250, 466, 1292
HgaI GACGC 1 cut(s) 1932
HhaI GCGC 2 cut(s) 25, 414
Hin1I GRCGYC 1 cut(s) 300
Hin6I GCGC 2 cut(s) 23, 412
HinP1I GCGC 2 cut(s) 23, 412
HincII GTYRAC 4 cut(s) 235, 586, 2305, 2413
HindII GTYRAC 4 cut(s) 235, 586, 2305, 2413
HindIII AAGCTT 1 cut(s) 324
HpaI GTTAAC 1 cut(s) 586
HpaII CCGG 3 cut(s) 250, 466, 1292
HphI GGTGA 6 cut(s) 19, 584, 1083, 1513, 1927, 2067
Hpy166II GTNNAC 9 cut(s) 235, 307, 369, 575, 586, 2069, 2305, 2413, 2433
Hpy188III TCNNGA 9 cut(s) 266, 275, 443, 1175, 1301, 1330, 1877, 2189, 2606
Hpy8I GTNNAC 9 cut(s) 235, 307, 369, 575, 586, 2069, 2305, 2413, 2433
Hpy99I CGWCG 2 cut(s) 203, 305
HpyAV CCTTC 9 cut(s) 295, 463, 831, 1327, 1516, 2186, 2347, 2491, 2579
HpyCH4III ACNGT 5 cut(s) 373, 623, 1921, 1971, 2066
HpyCH4IV ACGT 2 cut(s) 237, 300
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 2030, 2595, 2630
HpyF3I CTNAG 7 cut(s) 118, 127, 225, 417, 1269, 1425, 1674
HpySE526I ACGT 2 cut(s) 237, 300
Hsp92I GRCGYC 1 cut(s) 300
HspAI GCGC 2 cut(s) 23, 412
KflI GGGWCCC 1 cut(s) 2511
Ksp22I TGATCA 1 cut(s) 1954
KspAI GTTAAC 1 cut(s) 586
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 4 cut(s) 248, 971, 1063, 1470
Lsp1109I GCAGC 5 cut(s) 69, 408, 2544, 2611, 2614
LweI GCATC 5 cut(s) 703, 1135, 1699, 2353, 2607
MaeI CTAG 3 cut(s) 578, 2268, 2300
MaeII ACGT 2 cut(s) 237, 300
MaeIII GTNAC 6 cut(s) 1603, 1837, 1915, 1921, 2015, 2329
MbiI CCGCTC 1 cut(s) 2201
MflI RGATCY 4 cut(s) 121, 1439, 1561, 2195
MhlI GDGCHC 6 cut(s) 245, 294, 466, 614, 1068, 2012
MlsI TGGCCA 2 cut(s) 648, 2534
MluNI TGGCCA 2 cut(s) 648, 2534
MlyI GAGTC 4 cut(s) 181, 302, 1081, 1672
MmeI TCCRAC 3 cut(s) 721, 1041, 1264
Mox20I TGGCCA 2 cut(s) 648, 2534
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 2589
MscI TGGCCA 2 cut(s) 648, 2534
MseI TTAA 7 cut(s) 407, 585, 630, 693, 1125, 1772, 2376
MslI CAYNNNNRTG 2 cut(s) 915, 1581
Msp20I TGGCCA 2 cut(s) 648, 2534
MspCI CTTAAG 1 cut(s) 1771
MspI CCGG 3 cut(s) 250, 466, 1292
MspR9I CCNGG 1 cut(s) 466
Mva1269I GAATGC 4 cut(s) 716, 818, 1987, 2589
MvnI CGCG 1 cut(s) 2190
MwoI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 2030, 2595, 2630
NciI CCSGG 1 cut(s) 466
NcoI CCATGG 2 cut(s) 499, 2118
NdeI CATATG 1 cut(s) 1096
NlaIV GGNNCC 5 cut(s) 89, 554, 2197, 2512, 2513
NmuCI GTSAC 3 cut(s) 1915, 1921, 2329
NruI TCGCGA 1 cut(s) 2190
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 951
OliI CACNNNNGTG 1 cut(s) 915
PaeI GCATGC 1 cut(s) 951
PceI AGGCCT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 140
PctI GAATGC 4 cut(s) 716, 818, 1987, 2589
PdmI GAANNNNTTC 1 cut(s) 2589
PfeI GAWTC 9 cut(s) 38, 457, 655, 1054, 1171, 1400, 1421, 1880, 2114
PflMI CCANNNNNTGG 1 cut(s) 677
PkrI GCNGC 7 cut(s) 59, 423, 426, 2559, 2626, 2629, 2632
PleI GAGTC 4 cut(s) 181, 302, 1080, 1672
PpsI GAGTC 4 cut(s) 181, 302, 1080, 1672
PpuMI RGGWCCY 1 cut(s) 2511
PshAI GACNNNNGTC 1 cut(s) 191
PshBI ATTAAT 1 cut(s) 1125
PsiI TTATAA 1 cut(s) 1317
Psp124BI GAGCTC 1 cut(s) 614
Psp5II RGGWCCY 1 cut(s) 2511
PspFI CCCAGC 2 cut(s) 556, 2024
PspN4I GGNNCC 5 cut(s) 89, 554, 2197, 2512, 2513
PspPI GGNCC 3 cut(s) 252, 781, 2511
PspPPI RGGWCCY 1 cut(s) 2511
PsrI GAACNNNNNNTAC 2 cut(s) 536, 568
PstI CTGCAG 2 cut(s) 1786, 2632
PstNI CAGNNNCTG 4 cut(s) 1424, 1730, 1847, 1946
PsuI RGATCY 4 cut(s) 121, 1439, 1561, 2195
RruI TCGCGA 1 cut(s) 2190
RsaI GTAC 4 cut(s) 182, 933, 2340, 2434
RsaNI GTAC 4 cut(s) 181, 932, 2339, 2433
RseI CAYNNNNRTG 2 cut(s) 915, 1581
SacI GAGCTC 1 cut(s) 614
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 7 cut(s) 407, 585, 630, 693, 1125, 1772, 2376
SatI GCNGC 7 cut(s) 58, 422, 425, 2558, 2625, 2628, 2631
Sau96I GGNCC 3 cut(s) 252, 781, 2511
SbfI CCTGCAGG 1 cut(s) 1786
SchI GAGTC 4 cut(s) 181, 302, 1081, 1672
ScrFI CCNGG 1 cut(s) 466
SdaI CCTGCAGG 1 cut(s) 1786
SduI GDGCHC 6 cut(s) 245, 294, 466, 614, 1068, 2012
SfaNI GCATC 5 cut(s) 703, 1135, 1699, 2353, 2607
SfcI CTRYAG 2 cut(s) 1782, 2628
SinI GGWCC 2 cut(s) 781, 2511
SmiMI CAYNNNNRTG 2 cut(s) 915, 1581
SmlI CTYRAG 8 cut(s) 160, 401, 1069, 1659, 1680, 1771, 2011, 2390
SmoI CTYRAG 8 cut(s) 160, 401, 1069, 1659, 1680, 1771, 2011, 2390
SpeI ACTAGT 1 cut(s) 2299
SphI GCATGC 1 cut(s) 951
Sse8387I CCTGCAGG 1 cut(s) 1786
SseBI AGGCCT 1 cut(s) 835
SsiI CCGC 7 cut(s) 13, 294, 424, 1884, 2141, 2180, 2199
SspMI CTAG 3 cut(s) 578, 2268, 2300
SstI GAGCTC 1 cut(s) 614
StuI AGGCCT 1 cut(s) 835
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 4 cut(s) 151, 499, 1005, 2118
TaaI ACNGT 5 cut(s) 373, 623, 1921, 1971, 2066
TaiI ACGT 2 cut(s) 240, 303
TaqI TCGA 6 cut(s) 198, 288, 460, 1347, 2347, 2526
TatI WGTACW 2 cut(s) 931, 2432
TauI GCSGC 1 cut(s) 427
TfiI GAWTC 9 cut(s) 38, 457, 655, 1054, 1171, 1400, 1421, 1880, 2114
Tru1I TTAA 7 cut(s) 407, 585, 630, 693, 1125, 1772, 2376
Tru9I TTAA 7 cut(s) 407, 585, 630, 693, 1125, 1772, 2376
TscAI CASTG 6 cut(s) 229, 516, 1345, 1513, 1974, 2587
TseFI GTSAC 3 cut(s) 1915, 1921, 2329
TseI GCWGC 6 cut(s) 57, 421, 2557, 2624, 2627, 2630
Tsp45I GTSAC 3 cut(s) 1915, 1921, 2329
TspDTI ATGAA 9 cut(s) 942, 1032, 1035, 1374, 1506, 1995, 2183, 2384, 2472
TspGWI ACGGA 4 cut(s) 773, 866, 1883, 2441
TspRI CASTG 6 cut(s) 229, 516, 1345, 1513, 1974, 2587
Van91I CCANNNNNTGG 1 cut(s) 677
Vha464I CTTAAG 1 cut(s) 1771
VpaK11BI GGWCC 2 cut(s) 781, 2511
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 3 cut(s) 756, 791, 1538
XceI RCATGY 1 cut(s) 951
XmiI GTMKAC 2 cut(s) 306, 368
XmnI GAANNNNTTC 1 cut(s) 2589
XspI CTAG 3 cut(s) 578, 2268, 2300
ZraI GACGTC 1 cut(s) 301
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.