RLG00000026852

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000527
Physical Location & Seq
Forward (+)
6525100 .. 6528940
3841 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026852

Sequence Viewer

Length: 2391 bp
ATGAAGAACCCGAAGGAGGAGATCAGTTGTCCCCCATGTGAGTATTTCAGCCGCGGCGCGAAAGTCGAGGTGCGCGACGAGCAAGGTGAATGGTTTCCTGCTCATGTCCTGGACCCAAATCCAAACCCGCTTCGGAATCGGAAGCAGAAGTTGCTGGTGGTAGAGTATGAAACTCTCGTCTCCGACGACGACCCTTCGAAGCCGCTGACGGAGGAAATCGAGGTGGGTTTGCTCCGGCCTTCGCCGCCTGTGGAGGAGGAGGAGGAGGGGTTTGAGGTCAACGACGTCGTTGACGCGTTTAATTTGGACGCGTGGTGGCCCGGGGTGGTGATGCGTGTTGTGGGGGACAAGTACACGGTGGGGTTTAAGAGCCCGCCGGATTTGCTGGAACTTGGGCGCGGCGAGCTGCGGCGGCATTGGGATTGGCGCGGCGGCGAGTGGACTCGCGCCGGGAAGGAGATGATGATGCAATTGGATTTCAGCCCTGGGGAAGATGTTGAAGTGAATCTACACAAAGAACGTGTATGGTATGCTTGGATACCGGCAACTTATGTTGGTCCATTAGGGGGCAATTCTTTCCTGGTCCGTTATAAGGGTGCGAATAATGGTCATGAAAATGGTTTTGTTAAAGTTGTATCGGACTATCAGATTCGACCTCTTCCTCCGCAGCAAGAGGAAAGAGCTTTTGAATTGTTGGCAATGGTGGATGCATACCAAGATATGTGTTGGTGGGTTGGAGACATTACAAAAGTTCTTACAGGGGAAAGGTATGTTGTCACATTAAGATTCACAAAGCAGGAGAAGGAATTCAGATCGTCAGACCTGAGGCACCACTCAGAATGGATTGGTAGCAAATGGGTGTCTGACCCTAAGGCACTTGAAAATTCTATTGTCAATGAAGAACGATTAAGATATACAAGAAGCAATTACAGAAAATCCGAAGAAAAAACACCTTGTCATACTAAGTCTCAAATGAAGCAGTTGACTTGTCGTGTGAATAAATCACCTTACAGAAATACCATTAAGAAACTCAAATTATCTGAGCTTTCTAATGATGGTGCAACATTTTCAAGTCCAACTCCTTCAAAGAAGTTAAAAGGGGAACGCTCAGAAGACTCCTTATCTCTGTTGGCATTATTTGGCAGGGCAAAATCAATTAAAACATCCAACAGAAAAGGGCCTTTTGAAGATGGAGAGAAGTCCGAGCAACAACATGTTGGGCAACTGGATGGTAAAGTAACCACTCCCGTAAAAAGAAGGGTTGGACGACGAAAATCACAAGCTGATAGCCCATCCCCTGGTGTTAGACAGGGTACAAAGGCTAGACGACCAAAATTGCATGTCAAGAAGAGCAGTGGAATTGACAAAGCATATGAACAAAATCTTGAAGATGAGCATGTAATTAATGGAATTGAATCTTCTGGGATCGGTTCGGAGAATGAAGTAATGGGGGGATCAAAGGCTGGAAGCTCATGTCTGCTCGCTATGGAGGATAACCAACAACATAATGAAGATGGAGCAAGCAGTGGAATAAAACTGAAGAGAAAACGGCCTATGTTAGTTGTTGAACACTCAAAAGATCCAACAACAGGTTCTGGAGATTCGGTGAGAGATGGCCGAGCTGTTGCTTCTGTAGAGAAAGTTAAGCTGGCTGTCTCTGGAATTCCTGCAAAGGAACCTGATCATGATCAATCTTTATTCACAAAGGAAGGGAATTCTGTAATAACCGTGGAGGGATCAATAAGTGGAAGAAAAATTGAAACTGGAGTCAAAGGTGATGTGGAGCAAGAAGACGGTCCAAATTTGCCTTTTGTGAAAAGTATCCCACTTTGGGAAAAGATTGAATCAATGGAAGTATTCAAAAGATTTCCACAGAAGCCGCATTTTCATCCTCTACTGAAAACTAAAGAGGTGTTTCGTGAGGGATCGGCTTTCGGAAAAATGTTAGCCTTTAGCTCTTTGGTAGACCAGACCTCCAAGATACGGATTGGTGATCCTAGAGACGTCATTGATAGCAACTTGGAAGCGACAGTTGCATTGGAACTGCACGGCTTTGATGTTGATGCCATAAAGCGTCGTCTGACTGGGTTGCTAGACCTGAAAGCCGAGCTGGAGGAGCTTCAAAACCAGTCCAAAGTAGTTGAAGCCAAGATAACGGCGTGTAGACAAAACACAATCAAGGACACTGAAACTATCGGTAGAATTGACAAGGAGATCAAGGACTTAGAAGCAAACATCAAGGGCTTGAAGAAAAAACGGGCCACGGTAGTGTCAATGTGTTCGGCCAAAGATTCCGAGGTTAGCAAGTTGCAGTCAGAAGCAAAAGTTATCAGTGAAGGCATCGAGAGCATCCAACATGGTTTCGACATACTAGCTGCTGCTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

797

Amino Acids

89.25

Weight (kDa)

6.83

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 19 - 83 1.3e-09 Agenet domain
Agenet PF05641 163 - 222 1e-06 Agenet domain
DUF724 PF05266 602 - 795 5.7e-52 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AatII GACGTC 2 cut(s) 288, 2007
AccB1I GGYRCC 1 cut(s) 828
AccB7I CCANNNNNTGG 1 cut(s) 1298
AccI GTMKAC 2 cut(s) 1965, 2164
AccII CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
AclWI GGATC 6 cut(s) 1433, 1462, 1574, 1744, 1933, 1988
AcoI YGGCCR 2 cut(s) 1615, 2283
AcsI RAATTY 5 cut(s) 806, 883, 1662, 1714, 1801
AcuI CTGAAG 1 cut(s) 1559
AcyI GRCGYC 2 cut(s) 285, 2004
AfaI GTAC 2 cut(s) 353, 1315
AfiI CCNNNNNNNGG 7 cut(s) 16, 566, 592, 1298, 1589, 1831, 1983
AflIII ACRYGT 4 cut(s) 294, 309, 520, 1213
AjnI CCWGG 4 cut(s) 108, 484, 579, 1297
AjuI GAANNNNNNNTTGG 2 cut(s) 1576, 1608
AleI CACNNNNGTG 1 cut(s) 2267
Alw26I GTCTC 5 cut(s) 184, 732, 972, 1660, 1995
AlwI GGATC 6 cut(s) 1433, 1462, 1574, 1744, 1933, 1988
AlwNI CAGNNNCTG 1 cut(s) 1595
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 7 cut(s) 236, 317, 1178, 1550, 1615, 2259, 2283
ApeKI GCWGC 4 cut(s) 406, 667, 2375, 2378
ApoI RAATTY 5 cut(s) 806, 883, 1662, 1714, 1801
AseI ATTAAT 1 cut(s) 1404
Asp700I GAANNNNTTC 2 cut(s) 93, 806
AspLEI GCGC 5 cut(s) 59, 75, 399, 429, 449
AspS9I GGNCC 7 cut(s) 112, 318, 557, 583, 1178, 1796, 2259
AsuC2I CCSGG 3 cut(s) 321, 322, 451
AsuHPI GGTGA 6 cut(s) 98, 340, 996, 1618, 1787, 2003
AsuII TTCGAA 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 4 cut(s) 112, 557, 583, 1796
AxyI CCTNAGG 2 cut(s) 824, 870
BanI GGYRCC 1 cut(s) 828
BanII GRGCYC 1 cut(s) 374
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BbsI GAAGAC 2 cut(s) 1119, 1797
BbvI GCAGC 4 cut(s) 393, 679, 2362, 2365
BccI CCATC 6 cut(s) 1049, 1184, 1223, 1300, 1508, 1607
BceAI ACGGC 3 cut(s) 1565, 2065, 2172
BciT130I CCWGG 4 cut(s) 110, 486, 581, 1299
BciVI GTATCC 2 cut(s) 531, 1832
BclI TGATCA 2 cut(s) 1681, 1687
BcnI CCSGG 3 cut(s) 321, 322, 451
BcoDI GTCTC 5 cut(s) 184, 732, 972, 1660, 1995
BfaI CTAG 4 cut(s) 1323, 1998, 2093, 2372
BfmI CTRYAG 1 cut(s) 1632
BfuI GTATCC 2 cut(s) 531, 1832
Bme1390I CCNGG 7 cut(s) 110, 321, 322, 451, 486, 581, 1299
Bme18I GGWCC 4 cut(s) 112, 557, 583, 1796
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 7 cut(s) 112, 318, 557, 583, 1178, 1796, 2259
BmiI GGNNCC 3 cut(s) 114, 830, 1677
BmrFI CCNGG 7 cut(s) 110, 321, 322, 451, 486, 581, 1299
BmrI ACTGGG 1 cut(s) 2094
BmsI GCATC 6 cut(s) 321, 456, 697, 2053, 2349, 2358
BmuI ACTGGG 1 cut(s) 2094
BpiI GAAGAC 2 cut(s) 1119, 1797
BpmI CTGGAG 3 cut(s) 1617, 1785, 2132
Bpu14I TTCGAA 1 cut(s) 197
BpuMI CCSGG 3 cut(s) 321, 322, 451
BsaBI GATNNNNATC 1 cut(s) 1686
BsaHI GRCGYC 2 cut(s) 285, 2004
BsaJI CCNNGG 9 cut(s) 52, 320, 321, 484, 485, 1297, 1728, 2262, 2295
BsaXI ACNNNNNCTCC 6 cut(s) 254, 284, 1590, 1620, 1958, 1988
Bsc4I CCNNNNNNNGG 7 cut(s) 16, 566, 592, 1298, 1589, 1831, 1983
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 4 cut(s) 1230, 1768, 2089, 2128
Bse21I CCTNAGG 2 cut(s) 824, 870
Bse3DI GCAATG 1 cut(s) 705
Bse8I GATNNNNATC 1 cut(s) 1686
BseBI CCWGG 4 cut(s) 110, 486, 581, 1299
BseDI CCNNGG 9 cut(s) 52, 320, 321, 484, 485, 1297, 1728, 2262, 2295
BseGI GGATG 6 cut(s) 712, 1163, 1234, 1292, 1888, 2349
BseJI GATNNNNATC 1 cut(s) 1686
BseLI CCNNNNNNNGG 7 cut(s) 16, 566, 592, 1298, 1589, 1831, 1983
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 4 cut(s) 815, 849, 1032, 1122
BseNI ACTGG 4 cut(s) 1230, 1768, 2089, 2128
BseRI GAGGAG 6 cut(s) 32, 269, 272, 275, 278, 2129
BseXI GCAGC 4 cut(s) 393, 679, 2362, 2365
BsgI GTGCAG 1 cut(s) 2030
Bsh1236I CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
BshFI GGCC 7 cut(s) 238, 319, 1180, 1552, 1617, 2261, 2285
BshNI GGYRCC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 5 cut(s) 235, 321, 377, 450, 542
BslFI GGGAC 2 cut(s) 15, 359
BslI CCNNNNNNNGG 7 cut(s) 16, 566, 592, 1298, 1589, 1831, 1983
BsmAI GTCTC 5 cut(s) 184, 732, 972, 1660, 1995
BsmBI CGTCTC 2 cut(s) 184, 1995
BsmFI GGGAC 2 cut(s) 15, 359
BsnI GGCC 7 cut(s) 238, 319, 1180, 1552, 1617, 2261, 2285
BsoBI CYCGRG 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 197
Bsp1286I GDGCHC 1 cut(s) 374
BspANI GGCC 7 cut(s) 238, 319, 1180, 1552, 1617, 2261, 2285
BspCNI CTCAG 4 cut(s) 816, 848, 1033, 1121
BspFNI CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
BspHI TCATGA 2 cut(s) 610, 1684
BspLI GGNNCC 3 cut(s) 114, 830, 1677
BspPI GGATC 6 cut(s) 1433, 1462, 1574, 1744, 1933, 1988
BspQI GCTCTTC 1 cut(s) 1343
BspT104I TTCGAA 1 cut(s) 197
BspT107I GGYRCC 1 cut(s) 828
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 4 cut(s) 1230, 1768, 2089, 2128
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 9 cut(s) 52, 320, 321, 484, 485, 1297, 1728, 2262, 2295
BssNI GRCGYC 2 cut(s) 285, 2004
Bst2UI CCWGG 4 cut(s) 110, 486, 581, 1299
Bst4CI ACNGT 5 cut(s) 358, 1729, 1796, 2032, 2266
Bst6I CTCTTC 3 cut(s) 663, 1343, 1535
BstACI GRCGYC 2 cut(s) 285, 2004
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBI TTCGAA 1 cut(s) 197
BstC8I GCNNGC 5 cut(s) 374, 404, 1482, 1522, 1650
BstDEI CTNAG 7 cut(s) 824, 835, 870, 963, 1041, 1108, 2224
BstDSI CCRYGG 3 cut(s) 52, 1728, 2262
BstF5I GGATG 6 cut(s) 712, 1163, 1234, 1292, 1888, 2349
BstFNI CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
BstHHI GCGC 5 cut(s) 59, 75, 399, 429, 449
BstMAI GTCTC 5 cut(s) 184, 732, 972, 1660, 1995
BstMWI GCNNNNNNNGC 9 cut(s) 79, 151, 244, 382, 403, 412, 2033, 2116, 2346
BstNI CCWGG 4 cut(s) 110, 486, 581, 1299
BstNSI RCATGY 3 cut(s) 1217, 1343, 1400
BstSCI CCNGG 7 cut(s) 108, 319, 320, 449, 484, 579, 1297
BstSFI CTRYAG 1 cut(s) 1632
BstUI CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
BstV1I GCAGC 4 cut(s) 393, 679, 2362, 2365
BstV2I GAAGAC 2 cut(s) 1119, 1797
BstX2I RGATCY 1 cut(s) 1579
BstYI RGATCY 1 cut(s) 1579
Bsu36I CCTNAGG 2 cut(s) 824, 870
BsuI GTATCC 2 cut(s) 531, 1832
BsuRI GGCC 7 cut(s) 238, 319, 1180, 1552, 1617, 2261, 2285
BtgI CCRYGG 3 cut(s) 52, 1728, 2262
BtsCI GGATG 6 cut(s) 712, 1163, 1234, 1292, 1888, 2349
BtsI GCAGTG 2 cut(s) 1360, 1531
BtsIMutI CAGTG 4 cut(s) 1360, 1531, 2184, 2338
Cac8I GCNNGC 5 cut(s) 374, 404, 1482, 1522, 1650
CaiI CAGNNNCTG 1 cut(s) 1595
CciI TCATGA 2 cut(s) 610, 1684
CfoI GCGC 5 cut(s) 59, 75, 399, 429, 449
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 7 cut(s) 112, 318, 557, 583, 1178, 1796, 2259
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 320
CseI GACGC 3 cut(s) 302, 317, 2063
Csp6I GTAC 2 cut(s) 352, 1314
CviAII CATG 9 cut(s) 36, 104, 611, 1214, 1340, 1397, 1473, 1685, 2357
CviQI GTAC 2 cut(s) 352, 1314
DdeI CTNAG 7 cut(s) 824, 835, 870, 963, 1041, 1108, 2224
EaeI YGGCCR 2 cut(s) 1615, 2283
Eam1104I CTCTTC 3 cut(s) 663, 1343, 1535
EarI CTCTTC 3 cut(s) 663, 1343, 1535
Eco24I GRGCYC 1 cut(s) 374
Eco47I GGWCC 4 cut(s) 112, 557, 583, 1796
Eco57I CTGAAG 1 cut(s) 1559
Eco81I CCTNAGG 2 cut(s) 824, 870
Eco88I CYCGRG 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 1178
EcoRI GAATTC 3 cut(s) 806, 1662, 1714
EcoRII CCWGG 4 cut(s) 108, 484, 579, 1297
EcoT22I ATGCAT 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 374
Esp3I CGTCTC 2 cut(s) 184, 1995
FaeI CATG 9 cut(s) 39, 107, 614, 1217, 1343, 1400, 1476, 1688, 2360
FaqI GGGAC 2 cut(s) 15, 359
FatI CATG 9 cut(s) 35, 103, 610, 1213, 1339, 1396, 1472, 1684, 2356
FauI CCCGC 2 cut(s) 135, 381
FauNDI CATATG 1 cut(s) 1372
FbaI TGATCA 2 cut(s) 1681, 1687
FblI GTMKAC 2 cut(s) 1965, 2164
FokI GGATG 6 cut(s) 719, 1150, 1241, 1279, 1875, 2336
FriOI GRGCYC 1 cut(s) 374
FspBI CTAG 4 cut(s) 1323, 1998, 2093, 2372
GlaI GCGC 5 cut(s) 58, 74, 398, 428, 448
GsuI CTGGAG 3 cut(s) 1617, 1785, 2132
HaeIII GGCC 7 cut(s) 238, 319, 1180, 1552, 1617, 2261, 2285
HapII CCGG 5 cut(s) 235, 321, 377, 450, 542
HgaI GACGC 3 cut(s) 302, 317, 2063
HhaI GCGC 5 cut(s) 59, 75, 399, 429, 449
Hin1I GRCGYC 2 cut(s) 285, 2004
Hin1II CATG 9 cut(s) 39, 107, 614, 1217, 1343, 1400, 1476, 1688, 2360
Hin6I GCGC 5 cut(s) 57, 73, 397, 427, 447
HinP1I GCGC 5 cut(s) 57, 73, 397, 427, 447
HincII GTYRAC 3 cut(s) 280, 292, 984
HindII GTYRAC 3 cut(s) 280, 292, 984
HpaII CCGG 5 cut(s) 235, 321, 377, 450, 542
HphI GGTGA 6 cut(s) 98, 340, 996, 1618, 1787, 2003
Hpy166II GTNNAC 7 cut(s) 280, 292, 354, 441, 984, 1966, 2165
Hpy188III TCNNGA 8 cut(s) 611, 1345, 1385, 1596, 1659, 1685, 1919, 2344
Hpy8I GTNNAC 7 cut(s) 280, 292, 354, 441, 984, 1966, 2165
Hpy99I CGWCG 7 cut(s) 80, 188, 191, 287, 290, 1272, 2079
HpyAV CCTTC 9 cut(s) 7, 204, 249, 448, 796, 1092, 1251, 1703, 2330
HpyCH4III ACNGT 5 cut(s) 358, 1729, 1796, 2032, 2266
HpyCH4IV ACGT 3 cut(s) 285, 520, 2004
HpyCH4V TGCA 8 cut(s) 469, 710, 1061, 1339, 1670, 2036, 2047, 2311
HpyF10VI GCNNNNNNNGC 9 cut(s) 79, 151, 244, 382, 403, 412, 2033, 2116, 2346
HpyF3I CTNAG 7 cut(s) 824, 835, 870, 963, 1041, 1108, 2224
HpySE526I ACGT 3 cut(s) 285, 520, 2004
Hsp92I GRCGYC 2 cut(s) 285, 2004
Hsp92II CATG 9 cut(s) 39, 107, 614, 1217, 1343, 1400, 1476, 1688, 2360
HspAI GCGC 5 cut(s) 57, 73, 397, 427, 447
Ksp22I TGATCA 2 cut(s) 1681, 1687
KspI CCGCGG 1 cut(s) 55
LguI GCTCTTC 1 cut(s) 1343
LmnI GCTCC 5 cut(s) 237, 1517, 1783, 2116, 2386
Lsp1109I GCAGC 4 cut(s) 393, 679, 2362, 2365
LweI GCATC 6 cut(s) 321, 456, 697, 2053, 2349, 2358
MaeI CTAG 4 cut(s) 1323, 1998, 2093, 2372
MaeII ACGT 3 cut(s) 285, 520, 2004
MaeIII GTNAC 2 cut(s) 775, 1237
MfeI CAATTG 1 cut(s) 470
MflI RGATCY 1 cut(s) 1579
MhlI GDGCHC 1 cut(s) 374
MluI ACGCGT 2 cut(s) 294, 309
MlyI GAGTC 3 cut(s) 436, 1109, 1776
MmeI TCCRAC 7 cut(s) 207, 715, 1100, 1191, 1243, 1607, 2377
Mph1103I ATGCAT 1 cut(s) 712
MroXI GAANNNNTTC 2 cut(s) 93, 806
MslI CAYNNNNRTG 2 cut(s) 615, 2267
MspA1I CMGCKG 2 cut(s) 54, 205
MspI CCGG 5 cut(s) 235, 321, 377, 450, 542
MspR9I CCNGG 7 cut(s) 110, 321, 322, 451, 486, 581, 1299
MunI CAATTG 1 cut(s) 470
MvaI CCWGG 4 cut(s) 110, 486, 581, 1299
MvnI CGCG 8 cut(s) 54, 59, 75, 296, 311, 399, 429, 447
MwoI GCNNNNNNNGC 9 cut(s) 79, 151, 244, 382, 403, 412, 2033, 2116, 2346
NciI CCSGG 3 cut(s) 321, 322, 451
NdeI CATATG 1 cut(s) 1372
NlaIII CATG 9 cut(s) 39, 107, 614, 1217, 1343, 1400, 1476, 1688, 2360
NlaIV GGNNCC 3 cut(s) 114, 830, 1677
NmeAIII GCCGAG 2 cut(s) 1643, 2131
NmuCI GTSAC 1 cut(s) 775
NsiI ATGCAT 1 cut(s) 712
NspI RCATGY 3 cut(s) 1217, 1343, 1400
NspV TTCGAA 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 2267
PagI TCATGA 2 cut(s) 610, 1684
PasI CCCWGGG 1 cut(s) 485
PciI ACATGT 1 cut(s) 1213
PciSI GCTCTTC 1 cut(s) 1343
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 183
PdmI GAANNNNTTC 2 cut(s) 93, 806
PfeI GAWTC 8 cut(s) 136, 505, 649, 786, 1415, 1601, 1844, 2291
PflMI CCANNNNNTGG 1 cut(s) 1298
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 3 cut(s) 436, 1109, 1775
PpsI GAGTC 3 cut(s) 436, 1109, 1775
PscI ACATGT 1 cut(s) 1213
PshBI ATTAAT 1 cut(s) 1404
PsiI TTATAA 1 cut(s) 591
Psp6I CCWGG 4 cut(s) 108, 484, 579, 1297
PspGI CCWGG 4 cut(s) 108, 484, 579, 1297
PspN4I GGNNCC 3 cut(s) 114, 830, 1677
PspPI GGNCC 7 cut(s) 112, 318, 557, 583, 1178, 1796, 2259
PstNI CAGNNNCTG 1 cut(s) 1595
PsuI RGATCY 1 cut(s) 1579
RsaI GTAC 2 cut(s) 353, 1315
RsaNI GTAC 2 cut(s) 352, 1314
RseI CAYNNNNRTG 2 cut(s) 615, 2267
SacII CCGCGG 1 cut(s) 55
SapI GCTCTTC 1 cut(s) 1343
Sau96I GGNCC 7 cut(s) 112, 318, 557, 583, 1178, 1796, 2259
SchI GAGTC 3 cut(s) 436, 1109, 1776
ScrFI CCNGG 7 cut(s) 110, 321, 322, 451, 486, 581, 1299
SduI GDGCHC 1 cut(s) 374
SfaNI GCATC 6 cut(s) 321, 456, 697, 2053, 2349, 2358
SfcI CTRYAG 1 cut(s) 1632
Sfr303I CCGCGG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 197
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 4 cut(s) 112, 557, 583, 1796
SmaI CCCGGG 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 615, 2267
SspMI CTAG 4 cut(s) 1323, 1998, 2093, 2372
StyD4I CCNGG 7 cut(s) 108, 319, 320, 449, 484, 579, 1297
TaaI ACNGT 5 cut(s) 358, 1729, 1796, 2032, 2266
TaiI ACGT 3 cut(s) 288, 523, 2007
TaqI TCGA 6 cut(s) 66, 197, 219, 652, 2343, 2364
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 8 cut(s) 136, 505, 649, 786, 1415, 1601, 1844, 2291
TscAI CASTG 4 cut(s) 1360, 1531, 2191, 2338
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 4 cut(s) 406, 667, 2375, 2378
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 9 cut(s) 17, 183, 627, 912, 989, 1389, 1455, 1524, 1877
TspGWI ACGGA 3 cut(s) 224, 575, 1999
TspMI CCCGGG 1 cut(s) 320
TspRI CASTG 4 cut(s) 1360, 1531, 2191, 2338
Van91I CCANNNNNTGG 1 cut(s) 1298
VpaK11BI GGWCC 4 cut(s) 112, 557, 583, 1796
VspI ATTAAT 1 cut(s) 1404
XapI RAATTY 5 cut(s) 806, 883, 1662, 1714, 1801
XceI RCATGY 3 cut(s) 1217, 1343, 1400
XmaI CCCGGG 1 cut(s) 320
XmiI GTMKAC 2 cut(s) 1965, 2164
XmnI GAANNNNTTC 2 cut(s) 93, 806
XspI CTAG 4 cut(s) 1323, 1998, 2093, 2372
ZraI GACGTC 2 cut(s) 286, 2005
Zsp2I ATGCAT 1 cut(s) 712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.