Rh1AG384300

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000527
Physical Location & Seq
Reverse (-)
61541358 .. 61547422
6065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG384300.1

Sequence Viewer

Length: 2856 bp
ATGGCGAAACCGAGCAGCAATACTCGGACTTCGTTCTGGAGGCGAGGCGCGAAAGTGGAGGTGCGCACAGAAGAAGAAGGCTTCCACGGCGCGTGGTTCCCGGCGACGATCCTGGAGCCGCCGTCGAATCCTTCTTCGGTTCTCGGCAGGAAACGAAAGAGCTTCAACTCGAACAAGGTCTTGGTCCAGTACAACACCTTCGTCACGGAGAAGGACCCAACCAAGCCGCTGACGGAGTACACCAACGTCATGTTCCTCCGGCCGTCGCCGCCGGTGGAGGCTCCCGGGAAGACATTCGAGGCCGGCGAAGACGTGGACGCGTTTCACAACGACGCGTGGTGGCCGGGAGTGGTGATGAGTGTGGAAGGAGGGGACAGGTACAGGGTGGGGTTTAGGGACCCGCCGGAGTTGCTGGTGCTTGGCCGGAGTGAGTTGAGGAGTCACTTGGATTGGGTCAACGAGATTTGGTCCAGACCATACAAGAAGCGGATGAAGGAATCGATGTATAGTCCGGGGACTCCAATTGAAGTGAGACTTGTCAAAGAACATCTATGGTCTGCTTGGATTCCAGCAATTTATTCTGGTGCATTAGGGGGGAACTTGTTTTTGGTTCAATATAGGAGCCTAAAGAACAGTGATAAAGATGGGATTGCTACAGTGGCTGTATCTGACCATCAGATCCGACCTCGCCCTCCACGGCACAACGAAACAGGTTTTTGCTTGCTGGACATGGTGGATGCGTTCCATGATATGAGTTGGTGGGTTGGAAGTATTACAAATGTTCTTCCAGACAAAAAGTACATTGTCACTTTTAAACTTACAGATGAACAGAAAGAATTCAGTCGCTCAGAACTAAGGCTCCATATGGAATGGATTGACAAAAAATGGGAGTCTGACTTTTCGAGATTTGGGTCTGCGATGGATGATATAGAACAAACACAGCATACATTCACAAATTCCAGAAACTCTGCGGTAGCTACACTACTTAAAAGGTCACCTGCTACCAAGGATAGCACGGGAGAAGAAAATCCTTATGCTAGCTCACAGAAGAATCAGATGAAGCAGCTGGGTCCTTGCATTGATAAATCAGCTTATAGGAAAACCACAAATGAACTGAAACGACTGGACCAACCTAATGATGATGCAACATTTTTAAGTCCAACTCCTTCCAAGAAGTTAAAACGACATCCAGAAGAATCTGTTTCTATTGATGAAGTATATGTGAGGAGAACACCAATTAAAATATCCAATGAAAAAGGCCCTGTTGGAGATGGTGTGAAGACTGGGTCGCTGCTTGGAGAACCAGGTAGACAAAGAAAATCACAATTTGATGGGAGCAGTCCGCCACTGAAAATTCATGTTGCAGTCAAGAAGGGAAGTAGAATGGACAAGGCTGATGAAGAATGTATTGGCGATGAGCATGTAATGGACAAGACTGAATCTTCTGGTATTCAGTCAGACTCTGAAGTAACGAGAAGATCACTTGCTGGAAATTATAGTCTCCTGCATTTGAAGGAGATTAATCAACATGATGAAGATGGAGTGAGCAATCAGACAAAAGTGAAGGATTCTGAAGATTCAACTAGAAATGGGACAACAGAAGCATCTGGAATGGAAGTTCAGGTGACTGTGCCTGGATTGTCTGCAAAGCCTGGTGCTCATGATCAGCCAGTATCATTGTGTACAGATGAAATGCCTTCTGAATACACCATTGAGTGCTCAGTTAATCCGGTTGATGAAATGCATTCTCAATGCACTCTTAATCAGGATGGCAAAATGTTTCATGAAGGCACTATGGAGTGTGCAGGTAACCCTGCTGCAACTACCAACCAACAAATTCCAGTTGCTGAGGCTGGACTACCCGGAAAGACACCTACTCATGATCAGCCTCTACCATTGTGCATGTACAAGATGCATTCTGAAAAGACTATGGAGGGCTCAAGTTCTGAAGATCCAACAGAAGATGGAAAAGCTCAAGCTACTGCAATGGAAGTAGAGGTGGCTGGGACTGAAATATCCTCAGAGGCACCTCTTCCTGATCAACCTTTATTATTGTGTGACATGGACTCTGAAAACACTTTGGAGGGTAACCCCGCTGGGACTGCAAGAACAACCCTTCATGATCAATTTCAGGCATCAAGCATAGCTGAAATGTATTCTGAACATACTACTGAGGGCTCATGCAAGCGTGCTGGGACAGCAAAGCTTACTGGGAGACAAGGTGAAGCTGGAATTACCAGTGAAATTGAGCAAGAAGACACTCCACATCTGCCTTTCGTGAAAAAATCTCCATTTTGGAAAATGTTGGAATCCATGGATGCATTCAGAAGGTTTCCGCAACAGCCACATTTTAAGCCCTTACTGAAGGTTAAAATGTTATGCCGTGAAGGACTAGCCCTTGGAGGTATGCATATCTTTGCCGCTGTGGTCGAGTACACTTCGAAGCTGCAGGTTGATGATCCTAGTGACTCCATGAACGACATTTTGGAAGCTCTAGTTGAACTGGAACGGCTGGGATTTGATGTCAAGGCACTCCAGGTCCGACTGAATGAATTGCAAGACATGAAACTTAGGTTTGCAGAGCTTAAGAACAAGTCAGACGGAGTCAAAATCCTGATCACAAATTGTACGCATGAAAGAAGCAGAGGCTCTGAAGTTATCAGTGGGATGGATGAGGAGATCAATGACTTGGAGGAAAAGATCAGAGGGATACAGGCAAAAAGGGCAACGGCGGTGTCAGAGAGGGCGGCGAAAGATTCTGAGATCAGTATGTTGGAATCAGAAGCAAATGCTATCAATGAAGGCATTCAGAGCATCGAGCATGATTTCCAAAATCTAGCAGCAGCTGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

951

Amino Acids

106.0

Weight (kDa)

5.39

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 91 1.4e-13 Agenet domain
Agenet PF05641 172 - 232 2.6e-07 Agenet domain
DUF724 PF05266 757 - 949 8.4e-52 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1006
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 3 cut(s) 1006, 1796, 2440
AccB1I GGYRCC 1 cut(s) 2026
AccI GTMKAC 1 cut(s) 1309
AccII CGCG 4 cut(s) 50, 92, 320, 335
AclWI GGATC 4 cut(s) 103, 673, 1946, 2453
AcoI YGGCCR 3 cut(s) 260, 341, 421
AcsI RAATTY 4 cut(s) 836, 955, 1353, 1836
AcuI CTGAAG 5 cut(s) 1485, 1593, 1968, 2384, 2673
AfaI GTAC 8 cut(s) 191, 239, 380, 800, 1684, 1907, 2435, 2629
AflII CTTAAG 1 cut(s) 2585
AflIII ACRYGT 2 cut(s) 318, 333
AgsI TTSAA 6 cut(s) 166, 527, 614, 1513, 1581, 2501
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 5 cut(s) 111, 1303, 1633, 1651, 2535
AloI GAACNNNNNNTCC 4 cut(s) 843, 875, 1602, 1634
Alw21I GWGCWC 2 cut(s) 1660, 1721
Alw26I GTCTC 3 cut(s) 526, 1505, 2209
AlwI GGATC 4 cut(s) 103, 673, 1946, 2453
AlwNI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2846
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 5 cut(s) 260, 300, 341, 421, 1258
ApeKI GCWGC 8 cut(s) 15, 1063, 1291, 1817, 2446, 2840, 2843, 2846
ApoI RAATTY 4 cut(s) 836, 955, 1353, 1836
AseI ATTAAT 1 cut(s) 1521
Asp700I GAANNNNTTC 3 cut(s) 293, 836, 2805
AspLEI GCGC 3 cut(s) 50, 66, 92
AspS9I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2539
AsuC2I CCSGG 6 cut(s) 101, 285, 286, 345, 513, 1863
AsuHPI GGTGA 4 cut(s) 364, 987, 1636, 2234
AsuII TTCGAA 1 cut(s) 2441
AsuNHI GCTAGC 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2539
BanI GGYRCC 1 cut(s) 2026
BanII GRGCYC 2 cut(s) 1940, 2180
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbsI GAAGAC 4 cut(s) 296, 315, 1286, 2262
Bbv12I GWGCWC 2 cut(s) 1660, 1721
BbvCI CCTCAGC 1 cut(s) 1848
BbvI GCAGC 7 cut(s) 27, 1075, 1278, 1804, 2433, 2833, 2852
BccI CCATC 9 cut(s) 638, 681, 913, 1265, 1325, 1532, 1763, 1958, 2662
BceAI ACGGC 7 cut(s) 103, 106, 247, 713, 2367, 2525, 2745
BciT130I CCWGG 5 cut(s) 113, 1305, 1635, 1653, 2537
BciVI GTATCC 1 cut(s) 2703
BclI TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2616
BcnI CCSGG 6 cut(s) 101, 285, 286, 345, 513, 1863
BcoDI GTCTC 3 cut(s) 526, 1505, 2209
BfaI CTAG 6 cut(s) 1038, 1584, 2393, 2463, 2495, 2837
BfmI CTRYAG 2 cut(s) 654, 2447
BfrI CTTAAG 1 cut(s) 2585
BfuAI ACCTGC 3 cut(s) 1006, 1796, 2440
BfuI GTATCC 1 cut(s) 2703
Bme18I GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2539
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2539
BmrI ACTGGG 2 cut(s) 1293, 2220
BmsI GCATC 7 cut(s) 727, 1132, 1613, 1902, 2144, 2308, 2823
BmtI GCTAGC 1 cut(s) 1041
BmuI ACTGGG 2 cut(s) 1293, 2220
BpiI GAAGAC 4 cut(s) 296, 315, 1286, 2262
BpmI CTGGAG 3 cut(s) 58, 134, 2519
Bpu10I CCTNAGC 1 cut(s) 1848
Bpu14I TTCGAA 1 cut(s) 2441
BpuEI CTTGAG 2 cut(s) 1924, 1959
BpuMI CCSGG 6 cut(s) 101, 285, 286, 345, 513, 1863
Bsa29I ATCGAT 1 cut(s) 500
BsaJI CCNNGG 7 cut(s) 85, 284, 512, 695, 1005, 2313, 2398
BsaWI WCCGGW 1 cut(s) 1729
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 843, 873, 1260, 1290, 2206, 2236
Bse118I RCCGGY 2 cut(s) 271, 302
Bse1I ACTGG 8 cut(s) 187, 1128, 1288, 1670, 1841, 2215, 2238, 2508
Bse3DI GCAATG 1 cut(s) 1992
BseBI CCWGG 5 cut(s) 113, 1305, 1635, 1653, 2537
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 7 cut(s) 85, 284, 512, 695, 1005, 2313, 2398
BseGI GGATG 8 cut(s) 495, 742, 928, 1186, 1774, 2323, 2673, 2677
BseMI GCAATG 1 cut(s) 1992
BseMII CTCAG 6 cut(s) 861, 1734, 1839, 2034, 2163, 2751
BseNI ACTGG 8 cut(s) 187, 1128, 1288, 1670, 1841, 2215, 2238, 2508
BseRI GAGGAG 3 cut(s) 451, 1240, 2690
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 7 cut(s) 27, 1075, 1278, 1804, 2433, 2833, 2852
BseYI CCCAGC 5 cut(s) 1066, 2003, 2096, 2192, 2512
BsgI GTGCAG 1 cut(s) 1824
Bsh1236I CGCG 4 cut(s) 50, 92, 320, 335
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 5 cut(s) 262, 302, 343, 423, 1260
BshNI GGYRCC 1 cut(s) 2026
BshVI ATCGAT 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 1660, 1721
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
BsmAI GTCTC 3 cut(s) 526, 1505, 2209
BsmFI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
BsmI GAATGC 4 cut(s) 1744, 1915, 2321, 2805
BsnI GGCC 5 cut(s) 262, 302, 343, 423, 1260
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 2441
Bsp1286I GDGCHC 4 cut(s) 1660, 1721, 1940, 2180
Bsp1407I TGTACA 2 cut(s) 1682, 1905
Bsp19I CCATGG 1 cut(s) 2313
BspANI GGCC 5 cut(s) 262, 302, 343, 423, 1260
BspCNI CTCAG 6 cut(s) 860, 1733, 1840, 2033, 2164, 2752
BspDI ATCGAT 1 cut(s) 500
BspFNI CGCG 4 cut(s) 50, 92, 320, 335
BspHI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
BspMAI CTGCAG 1 cut(s) 2451
BspMI ACCTGC 3 cut(s) 1006, 1796, 2440
BspOI GCTAGC 1 cut(s) 1041
BspPI GGATC 4 cut(s) 103, 673, 1946, 2453
BspT104I TTCGAA 1 cut(s) 2441
BspT107I GGYRCC 1 cut(s) 2026
BspTI CTTAAG 1 cut(s) 2585
BsrDI GCAATG 1 cut(s) 1992
BsrFI RCCGGY 2 cut(s) 271, 302
BsrGI TGTACA 2 cut(s) 1682, 1905
BsrI ACTGG 8 cut(s) 187, 1128, 1288, 1670, 1841, 2215, 2238, 2508
BssAI RCCGGY 2 cut(s) 271, 302
BssECI CCNNGG 7 cut(s) 85, 284, 512, 695, 1005, 2313, 2398
BssT1I CCWWGG 3 cut(s) 1005, 2313, 2398
Bst2UI CCWGG 5 cut(s) 113, 1305, 1635, 1653, 2537
Bst4CI ACNGT 3 cut(s) 635, 658, 1630
Bst6I CTCTTC 1 cut(s) 2037
BstAFI CTTAAG 1 cut(s) 2585
BstAPI GCANNNNNTGC 1 cut(s) 2846
BstAUI TGTACA 2 cut(s) 1682, 1905
BstBI TTCGAA 1 cut(s) 2441
BstC8I GCNNGC 5 cut(s) 304, 722, 1039, 2186, 2190
BstDEI CTNAG 8 cut(s) 847, 854, 1720, 1848, 2020, 2172, 2570, 2760
BstDSI CCRYGG 3 cut(s) 85, 695, 2313
BstEII GGTNACC 3 cut(s) 993, 1808, 2087
BstF5I GGATG 8 cut(s) 495, 742, 928, 1186, 1774, 2323, 2673, 2677
BstFNI CGCG 4 cut(s) 50, 92, 320, 335
BstHHI GCGC 3 cut(s) 50, 66, 92
BstMAI GTCTC 3 cut(s) 526, 1505, 2209
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 9 cut(s) 87, 268, 409, 659, 2102, 2198, 2723, 2811, 2846
BstNI CCWGG 5 cut(s) 113, 1305, 1635, 1653, 2537
BstNSI RCATGY 2 cut(s) 1424, 1906
BstPI GGTNACC 3 cut(s) 993, 1808, 2087
BstSFI CTRYAG 2 cut(s) 654, 2447
BstUI CGCG 4 cut(s) 50, 92, 320, 335
BstV1I GCAGC 7 cut(s) 27, 1075, 1278, 1804, 2433, 2833, 2852
BstV2I GAAGAC 4 cut(s) 296, 315, 1286, 2262
BstX2I RGATCY 2 cut(s) 678, 1951
BstYI RGATCY 2 cut(s) 678, 1951
BstZI CGGCCG 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 500
BsuI GTATCC 1 cut(s) 2703
BsuRI GGCC 5 cut(s) 262, 302, 343, 423, 1260
BsuTUI ATCGAT 1 cut(s) 500
BtgI CCRYGG 3 cut(s) 85, 695, 2313
BtgZI GCGATG 2 cut(s) 932, 1428
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 8 cut(s) 495, 742, 928, 1186, 1774, 2323, 2673, 2677
BtsIMutI CAGTG 5 cut(s) 640, 663, 1346, 2245, 2668
BveI ACCTGC 3 cut(s) 1006, 1796, 2440
Cac8I GCNNGC 5 cut(s) 304, 722, 1039, 2186, 2190
CaiI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2846
CciI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
CfoI GCGC 3 cut(s) 50, 66, 92
Cfr10I RCCGGY 2 cut(s) 271, 302
Cfr13I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2539
Cfr9I CCCGGG 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 500
CseI GACGC 2 cut(s) 326, 341
CsiI ACCWGGT 1 cut(s) 1303
Csp6I GTAC 8 cut(s) 190, 238, 379, 799, 1683, 1906, 2434, 2628
CviQI GTAC 8 cut(s) 190, 238, 379, 799, 1683, 1906, 2434, 2628
DdeI CTNAG 8 cut(s) 847, 854, 1720, 1848, 2020, 2172, 2570, 2760
DraI TTTAAA 1 cut(s) 814
EaeI YGGCCR 3 cut(s) 260, 341, 421
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 2037
EarI CTCTTC 1 cut(s) 2037
EciI GGCGGA 1 cut(s) 1332
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 3 cut(s) 1005, 2313, 2398
Eco24I GRGCYC 2 cut(s) 1940, 2180
Eco47I GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2539
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 5 cut(s) 1485, 1593, 1968, 2384, 2673
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 3 cut(s) 993, 1808, 2087
EcoO109I RGGNCCY 4 cut(s) 214, 397, 1070, 1259
EcoO65I GGTNACC 3 cut(s) 993, 1808, 2087
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 5 cut(s) 111, 1303, 1633, 1651, 2535
EcoT14I CCWWGG 3 cut(s) 1005, 2313, 2398
EcoT22I ATGCAT 4 cut(s) 1746, 1917, 2323, 2412
EcoT38I GRGCYC 2 cut(s) 1940, 2180
ErhI CCWWGG 3 cut(s) 1005, 2313, 2398
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
FauI CCCGC 2 cut(s) 408, 2101
FauNDI CATATG 1 cut(s) 864
FbaI TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2616
FblI GTMKAC 1 cut(s) 1309
FokI GGATG 8 cut(s) 502, 749, 935, 1173, 1781, 2330, 2680, 2684
FriOI GRGCYC 2 cut(s) 1940, 2180
FspAI RTGCGCAY 1 cut(s) 65
FspBI CTAG 6 cut(s) 1038, 1584, 2393, 2463, 2495, 2837
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 3 cut(s) 49, 65, 91
GsaI CCCAGC 5 cut(s) 1070, 2007, 2100, 2196, 2516
GsuI CTGGAG 3 cut(s) 58, 134, 2519
HaeIII GGCC 5 cut(s) 262, 302, 343, 423, 1260
HgaI GACGC 2 cut(s) 326, 341
HhaI GCGC 3 cut(s) 50, 66, 92
Hin6I GCGC 3 cut(s) 48, 64, 90
HinP1I GCGC 3 cut(s) 48, 64, 90
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 2204
HphI GGTGA 4 cut(s) 364, 987, 1636, 2234
Hpy166II GTNNAC 6 cut(s) 240, 316, 457, 1310, 1683, 2436
Hpy8I GTNNAC 6 cut(s) 240, 316, 457, 1310, 1683, 2436
Hpy99I CGWCG 4 cut(s) 109, 127, 268, 335
HpyCH4III ACNGT 3 cut(s) 635, 658, 1630
HpyCH4IV ACGT 2 cut(s) 246, 312
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 268, 409, 659, 2102, 2198, 2723, 2811, 2846
HpyF3I CTNAG 8 cut(s) 847, 854, 1720, 1848, 2020, 2172, 2570, 2760
HpySE526I ACGT 2 cut(s) 246, 312
HspAI GCGC 3 cut(s) 48, 64, 90
KflI GGGWCCC 1 cut(s) 397
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2616
LmnI GCTCC 5 cut(s) 115, 286, 621, 864, 1335
Lsp1109I GCAGC 7 cut(s) 27, 1075, 1278, 1804, 2433, 2833, 2852
LweI GCATC 7 cut(s) 727, 1132, 1613, 1902, 2144, 2308, 2823
MabI ACCWGGT 1 cut(s) 1303
MaeI CTAG 6 cut(s) 1038, 1584, 2393, 2463, 2495, 2837
MaeII ACGT 2 cut(s) 246, 312
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 2 cut(s) 678, 1951
MhlI GDGCHC 4 cut(s) 1660, 1721, 1940, 2180
MluI ACGCGT 2 cut(s) 318, 333
MlyI GAGTC 7 cut(s) 448, 511, 899, 1454, 2060, 2462, 2613
MmeI TCCRAC 8 cut(s) 706, 745, 1184, 1246, 1979, 2286, 2566, 2754
Mph1103I ATGCAT 4 cut(s) 1746, 1917, 2323, 2412
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 3 cut(s) 293, 836, 2805
MspA1I CMGCKG 5 cut(s) 229, 1066, 2096, 2423, 2846
MspCI CTTAAG 1 cut(s) 2585
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 4 cut(s) 1744, 1915, 2321, 2805
MvaI CCWGG 5 cut(s) 113, 1305, 1635, 1653, 2537
MvnI CGCG 4 cut(s) 50, 92, 320, 335
MwoI GCNNNNNNNGC 9 cut(s) 87, 268, 409, 659, 2102, 2198, 2723, 2811, 2846
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 6 cut(s) 101, 285, 286, 345, 513, 1863
NcoI CCATGG 1 cut(s) 2313
NdeI CATATG 1 cut(s) 864
NgoMIV GCCGGC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 1037
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 7 cut(s) 202, 440, 805, 993, 1624, 2057, 2465
NsbI TGCGCA 1 cut(s) 65
NsiI ATGCAT 4 cut(s) 1746, 1917, 2323, 2412
NspI RCATGY 2 cut(s) 1424, 1906
NspV TTCGAA 1 cut(s) 2441
PagI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
PaqCI CACCTGC 1 cut(s) 1006
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 4 cut(s) 1744, 1915, 2321, 2805
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 3 cut(s) 293, 836, 2805
PflFI GACNNNGTC 2 cut(s) 1285, 2603
PfoI TCCNGGA 1 cut(s) 111
PleI GAGTC 7 cut(s) 447, 511, 898, 1454, 2060, 2462, 2612
PpsI GAGTC 7 cut(s) 447, 511, 898, 1454, 2060, 2462, 2612
PpuMI RGGWCCY 3 cut(s) 214, 397, 1070
PshBI ATTAAT 1 cut(s) 1521
Psp5II RGGWCCY 3 cut(s) 214, 397, 1070
Psp6I CCWGG 5 cut(s) 111, 1303, 1633, 1651, 2535
PspEI GGTNACC 3 cut(s) 993, 1808, 2087
PspFI CCCAGC 5 cut(s) 1066, 2003, 2096, 2192, 2512
PspGI CCWGG 5 cut(s) 111, 1303, 1633, 1651, 2535
PspPI GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2539
PspPPI RGGWCCY 3 cut(s) 214, 397, 1070
PstI CTGCAG 1 cut(s) 2451
PstNI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2846
PsuI RGATCY 2 cut(s) 678, 1951
PsyI GACNNNGTC 2 cut(s) 1285, 2603
PvuII CAGCTG 2 cut(s) 1066, 2846
RsaI GTAC 8 cut(s) 191, 239, 380, 800, 1684, 1907, 2435, 2629
RsaNI GTAC 8 cut(s) 190, 238, 379, 799, 1683, 1906, 2434, 2628
Sau96I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2539
SchI GAGTC 7 cut(s) 448, 511, 899, 1454, 2060, 2462, 2613
SduI GDGCHC 4 cut(s) 1660, 1721, 1940, 2180
SexAI ACCWGGT 1 cut(s) 1303
SfaNI GCATC 7 cut(s) 727, 1132, 1613, 1902, 2144, 2308, 2823
SfcI CTRYAG 2 cut(s) 654, 2447
SfuI TTCGAA 1 cut(s) 2441
SgrAI CRCCGGYG 1 cut(s) 271
SinI GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2539
SmaI CCCGGG 1 cut(s) 286
SmlI CTYRAG 3 cut(s) 1939, 1974, 2585
SmoI CTYRAG 3 cut(s) 1939, 1974, 2585
SspMI CTAG 6 cut(s) 1038, 1584, 2393, 2463, 2495, 2837
StyI CCWWGG 3 cut(s) 1005, 2313, 2398
TaaI ACNGT 3 cut(s) 635, 658, 1630
TaiI ACGT 2 cut(s) 249, 315
TaqI TCGA 8 cut(s) 125, 170, 297, 500, 902, 2430, 2441, 2817
TatI WGTACW 6 cut(s) 189, 237, 798, 1682, 1905, 2433
TauI GCSGC 5 cut(s) 121, 229, 271, 2423, 2750
TscAI CASTG 5 cut(s) 640, 663, 1353, 2245, 2668
TseFI GTSAC 7 cut(s) 202, 440, 805, 993, 1624, 2057, 2465
TseI GCWGC 8 cut(s) 15, 1063, 1291, 1817, 2446, 2840, 2843, 2846
Tsp45I GTSAC 7 cut(s) 202, 440, 805, 993, 1624, 2057, 2465
TspGWI ACGGA 3 cut(s) 221, 248, 2616
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 5 cut(s) 640, 663, 1353, 2245, 2668
Tth111I GACNNNGTC 2 cut(s) 1285, 2603
Vha464I CTTAAG 1 cut(s) 2585
VpaK11BI GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2539
VspI ATTAAT 1 cut(s) 1521
XapI RAATTY 4 cut(s) 836, 955, 1353, 1836
XceI RCATGY 2 cut(s) 1424, 1906
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1309
XmnI GAANNNNTTC 3 cut(s) 293, 836, 2805
XspI CTAG 6 cut(s) 1038, 1584, 2393, 2463, 2495, 2837
Zsp2I ATGCAT 4 cut(s) 1746, 1917, 2323, 2412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.