Rh1DG379300

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000527
Physical Location & Seq
Reverse (-)
60115750 .. 60121856
6107 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG379300.1

Sequence Viewer

Length: 2868 bp
ATGGCGAAACCGAGCAGCAATACTCGGACTTGGTTCTGGAACCGAGGCGCGAAAGTGGAGGTGCGCACAGAAGAAGAAGGCTTCCACGGCGCGTGGTTCCCGGCGACGATCCTGGAGCCGCCGTCGAATCCTTCTTCGGTTCTCAGCAGGAAACGAAAGAGCTTCAACTCGAACAAGGTCTTGGTCCAGTACAACACCTTCGTCACGGAGAAGGACCCAACCAAGCCGCTGACGGAGTACACCAACGTCATGTTCCTCCGGCCGTCGCCGCCGGTGGAGGCTCCCGGGAAGACATTCGAGGCCGGCGAAGACGTGGACGCGTTTCACAACGACGCGTGGTGGCCGGGAGTGGTGATGAGTGTGGAAGGAGGGGACAGGTACAGGGTGGGGTTTAGGGACCCGCCGGAGTTGCTGGTGCTTGGCCGGAGTGAGTTGAGGAGTCACTTGGATTGGGTCAACGAGATTTGGTCCAGACCATACAAGAAGCGGATGAAGGAATCGATGTATAGTCCGGGGACTCCAATTGAAGTGAGACTTGTCAAAGAACATCTATGGTCTGCTTGGATTCCAGCAATTTATGCTGGTGCATTAGGGGGGAACTTGTTTTTGGTTCAATATAAGAGCCTAAAGAACAGTGATAAAGATGGGATTGCTACAGTGGCTGTATCTGACCATCAGATCCGACCTCGCCCTCCACGGCACAACGAAACAGGTTTTTGCTTACTGGACATGGTGGATGCGTTCCATGATATGAGTTGGTGGGTTGGAAGTATTACAAATGTTCTTCCAGACAAAAAGTACATTGTCACTTTTAAACTTACAGATGAACAGAAAGAATTCAGTCACTCAGAACTAAGGCTCCATATGGAATGGATTGACAAAAAATGGGAGTCTGACTTTTCGAGATTTGGGTCTGTGATGGATGATATAGAACAAGCACAGCATACATTCACAAATTCCAGAAACTCTGCGGTAGCTACACCACTTAAAAGGTCACCTGCTACCAAGGATAGCACGGGAGAAGAAAATCCTTATGCTAGCTCACAGAAGAATCAGATGAAGCAGCTGGGTCCTTGCATTGATAAATCAGCTTATAGGAAAACCACAAATGAACTGAAACGACTGGACCAACCTAATGATGATGCAACATTTTTAAGTCCAACTCCTTCCAAGAAGTTAAAACGACATCCAGAAGAATCTGTTTCTATTGATGAAGTATATGTGAGGAGAACACCGATTAAAATATCCAAAGAAAAAGGCCCTGTTGGAGATGGTGTGAAGACTGGGTCGCTGCTTGGAGAACCAGGTAGACAAAGAAAATCACAATTTGATGGGAGCAGTCCACCACTGAAAATTCATGTTGCAGTCAAGAAGGGAAGTAGAATGGACAAGGCTGATGAAGAATGTATTGGCGATGAGCATGTAATGGACAAGACTGAATCTTCTGGTATTCAGTCAGACTCTGAAGTAACGAGAAGATCACTTACTGGAAATTATAGTCTCCTGCATTTGAAGGAGATTAATCAACATGATGAAGATGGAGTGAGCAATCAGACAAAAGTGAAGGATTCTGAAGATTCAACAAGAAATGGGACAACAGAAGCATCTGGAATGGAAATTCAGGTGACTGTGCCTGGATTGTCTGCAAAGCCTGCTGCTCATGATCAGCCAGTATCATTGTGTACAGATGAAATGCCTTCTGAATACACCATTGAGTGCTCAGTTAATCCGGTTGATGAAATGCATTCTCAATGCACTCTTAATCAGGATGACAAAATGTTTCATGAAGGCACTATGGAGTGTGCAGGTAACCCTGCTGCAACTACCAACCAACAAATTCCAGTTTCTGAGGCTGGACTACCCGGAAAGACACCTACTCATGATCAGCCTCTACCATTGTGCTTATACAAGATGCATTCTGAAAAGACTATGGATGGCTCAAGTTCTGAAGATCCAACAGAAGATGGAAAAGCTCAAGCTACTGCAATGGAAGTAGAGGTGGCTGGGACTGAAATATCCTCAGAGACACCTCTTCCTGATCAACCTTTATTATTGTGTGACATGGACTCTGAAAACACTGTGGAGGGTAACCCGGCTGGGACTGCAAGAACATCCCTTCATGATCAATTTCAGGCATCAAGCATAGCTGAAATGTATTCTGAACATACTACTGAGGGCTCATGCAAGCGTGCTGGGACAACAAAGCAACAATATGAGGTTACTGGGAGGCAAGGTGAAGCTGGAATTACCAGTGACATTGAGCAAGAAGACACTCCACATCTGCCTTTTATGAAAAAATCTCCATTTTGGAAAATGTTGGAATCCATGGATGCATTCAAAAGGTGTCCGCAACGGCCACATTTTAAGCCCTTACTGAAGGTTAAAATGTTCTGCCGTGAAGGACTAGCCCTTGGAGGTATGCATATCTTTGCCGCTGTGGTCGAGTACACTTCGAAGCTGCAGGTTGATGATCCTAGTGACTCCATCAACGACATTTTGGAAGCTCTAGTTGAACTGGAACGGCTGGGATTTGATGTCAAGGCACTCCAGGTCCGACTGAATGAATTGCAAGACATGAAACTTAGGTTTGCAGAGCTTAAGAACAAGTCAGACGGAGTCAAAATCCTGATCACAAATTGTACGCATGAAAGAAGCAAAGGCTCTGAAGTTATCAGTGGGATGGATGCGGAGATCAATGACTTGGAGGAAAAGATCAGAGGGATACAGGCAAAAAGGGCAAAGGCGGTGTCAGAGAGGGCGGCGAAAGATTCTGAAATCAGTATGTTGGAATCAGAAGCAAATGCTATCAATGAAGGCATTCAGAGCATCGAGCATGATTTCCAAAATCTAGCAGCAGCTGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

955

Amino Acids

106.62

Weight (kDa)

5.42

Isoelectric Point (pI)

51.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 91 1.4e-13 Agenet domain
Agenet PF05641 172 - 232 2e-07 Agenet domain
DUF724 PF05266 761 - 953 1.4e-51 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1006
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 3 cut(s) 1006, 1796, 2452
AccI GTMKAC 1 cut(s) 1309
AccII CGCG 4 cut(s) 50, 92, 320, 335
AclWI GGATC 4 cut(s) 103, 673, 1946, 2465
AcoI YGGCCR 4 cut(s) 260, 341, 421, 2354
AcsI RAATTY 5 cut(s) 836, 955, 1353, 1617, 1836
AcuI CTGAAG 5 cut(s) 1485, 1593, 1968, 2396, 2685
AfaI GTAC 7 cut(s) 191, 239, 380, 800, 1684, 2447, 2641
AflII CTTAAG 1 cut(s) 2597
AflIII ACRYGT 2 cut(s) 318, 333
AgsI TTSAA 7 cut(s) 166, 527, 614, 1513, 1581, 2338, 2513
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 4 cut(s) 111, 1303, 1633, 2547
AjuI GAANNNNNNNTTGG 8 cut(s) 164, 196, 236, 268, 590, 622, 1392, 1424
AloI GAACNNNNNNTCC 2 cut(s) 843, 875
Alw21I GWGCWC 1 cut(s) 1721
Alw26I GTCTC 3 cut(s) 526, 1505, 2018
AlwI GGATC 4 cut(s) 103, 673, 1946, 2465
AlwNI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2858
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 6 cut(s) 260, 300, 341, 421, 1258, 2354
ApeKI GCWGC 9 cut(s) 15, 1063, 1291, 1655, 1817, 2458, 2852, 2855, 2858
ApoI RAATTY 5 cut(s) 836, 955, 1353, 1617, 1836
ArsI GACNNNNNNTTYG 2 cut(s) 2732, 2764
AseI ATTAAT 1 cut(s) 1521
Asp700I GAANNNNTTC 3 cut(s) 293, 836, 2817
AspLEI GCGC 3 cut(s) 50, 66, 92
AspS9I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2551
AsuC2I CCSGG 7 cut(s) 101, 285, 286, 345, 513, 1863, 2093
AsuHPI GGTGA 4 cut(s) 364, 987, 1636, 2246
AsuII TTCGAA 1 cut(s) 2453
AsuNHI GCTAGC 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2551
BanII GRGCYC 1 cut(s) 2180
BarI GAAGNNNNNNTAC 4 cut(s) 182, 214, 1468, 1500
BbsI GAAGAC 4 cut(s) 296, 315, 1286, 2274
Bbv12I GWGCWC 1 cut(s) 1721
BbvI GCAGC 8 cut(s) 27, 1075, 1278, 1642, 1804, 2445, 2845, 2864
BceAI ACGGC 7 cut(s) 103, 106, 247, 713, 2369, 2379, 2537
BciT130I CCWGG 4 cut(s) 113, 1305, 1635, 2549
BciVI GTATCC 1 cut(s) 2715
BclI TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2628
BcnI CCSGG 7 cut(s) 101, 285, 286, 345, 513, 1863, 2093
BcoDI GTCTC 3 cut(s) 526, 1505, 2018
BfaI CTAG 5 cut(s) 1038, 2405, 2475, 2507, 2849
BfmI CTRYAG 2 cut(s) 654, 2459
BfrI CTTAAG 1 cut(s) 2597
BfuAI ACCTGC 3 cut(s) 1006, 1796, 2452
BfuI GTATCC 1 cut(s) 2715
Bme18I GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2551
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2551
BmiI GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 860, 1071
BmrI ACTGGG 2 cut(s) 1293, 2232
BmsI GCATC 8 cut(s) 727, 1132, 1613, 1902, 2144, 2320, 2674, 2835
BmtI GCTAGC 1 cut(s) 1041
BmuI ACTGGG 2 cut(s) 1293, 2232
BpiI GAAGAC 4 cut(s) 296, 315, 1286, 2274
BpmI CTGGAG 2 cut(s) 134, 2531
Bpu14I TTCGAA 1 cut(s) 2453
BpuEI CTTGAG 2 cut(s) 1924, 1959
BpuMI CCSGG 7 cut(s) 101, 285, 286, 345, 513, 1863, 2093
Bsa29I ATCGAT 1 cut(s) 500
BsaJI CCNNGG 8 cut(s) 43, 85, 284, 512, 695, 1005, 2325, 2410
BsaWI WCCGGW 1 cut(s) 1729
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 843, 873, 1260, 1290, 2218, 2248
Bse118I RCCGGY 2 cut(s) 271, 302
Bse3DI GCAATG 1 cut(s) 1992
BseBI CCWGG 4 cut(s) 113, 1305, 1635, 2549
BseCI ATCGAT 1 cut(s) 500
BseDI CCNNGG 8 cut(s) 43, 85, 284, 512, 695, 1005, 2325, 2410
BseMI GCAATG 1 cut(s) 1992
BseMII CTCAG 6 cut(s) 157, 861, 1734, 1839, 2034, 2163
BseRI GAGGAG 2 cut(s) 451, 1240
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 8 cut(s) 27, 1075, 1278, 1642, 1804, 2445, 2845, 2864
BseYI CCCAGC 5 cut(s) 1066, 2003, 2096, 2192, 2524
BsgI GTGCAG 1 cut(s) 1824
Bsh1236I CGCG 4 cut(s) 50, 92, 320, 335
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 6 cut(s) 262, 302, 343, 423, 1260, 2356
BshVI ATCGAT 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 1721
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
BsmAI GTCTC 3 cut(s) 526, 1505, 2018
BsmFI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
BsmI GAATGC 4 cut(s) 1744, 1915, 2333, 2817
BsnI GGCC 6 cut(s) 262, 302, 343, 423, 1260, 2356
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 2453
Bsp1286I GDGCHC 2 cut(s) 1721, 2180
Bsp1407I TGTACA 1 cut(s) 1682
Bsp19I CCATGG 1 cut(s) 2325
BspANI GGCC 6 cut(s) 262, 302, 343, 423, 1260, 2356
BspCNI CTCAG 6 cut(s) 156, 860, 1733, 1840, 2033, 2164
BspDI ATCGAT 1 cut(s) 500
BspFNI CGCG 4 cut(s) 50, 92, 320, 335
BspHI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
BspLI GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 860, 1071
BspMAI CTGCAG 1 cut(s) 2463
BspMI ACCTGC 3 cut(s) 1006, 1796, 2452
BspOI GCTAGC 1 cut(s) 1041
BspPI GGATC 4 cut(s) 103, 673, 1946, 2465
BspT104I TTCGAA 1 cut(s) 2453
BspTI CTTAAG 1 cut(s) 2597
BsrDI GCAATG 1 cut(s) 1992
BsrFI RCCGGY 2 cut(s) 271, 302
BsrGI TGTACA 1 cut(s) 1682
BssAI RCCGGY 2 cut(s) 271, 302
BssECI CCNNGG 8 cut(s) 43, 85, 284, 512, 695, 1005, 2325, 2410
BssT1I CCWWGG 3 cut(s) 1005, 2325, 2410
Bst2UI CCWGG 4 cut(s) 113, 1305, 1635, 2549
Bst4CI ACNGT 4 cut(s) 635, 658, 1630, 2080
Bst6I CTCTTC 1 cut(s) 2037
BstAFI CTTAAG 1 cut(s) 2597
BstAPI GCANNNNNTGC 3 cut(s) 578, 1652, 2858
BstAUI TGTACA 1 cut(s) 1682
BstBI TTCGAA 1 cut(s) 2453
BstC8I GCNNGC 5 cut(s) 304, 1039, 1653, 2186, 2190
BstDEI CTNAG 8 cut(s) 143, 847, 854, 1720, 1848, 2020, 2172, 2582
BstDSI CCRYGG 3 cut(s) 85, 695, 2325
BstEII GGTNACC 3 cut(s) 993, 1808, 2087
BstFNI CGCG 4 cut(s) 50, 92, 320, 335
BstHHI GCGC 3 cut(s) 50, 66, 92
BstMAI GTCTC 3 cut(s) 526, 1505, 2018
BstMCI CGRYCG 1 cut(s) 263
BstNI CCWGG 4 cut(s) 113, 1305, 1635, 2549
BstNSI RCATGY 1 cut(s) 1424
BstPI GGTNACC 3 cut(s) 993, 1808, 2087
BstSFI CTRYAG 2 cut(s) 654, 2459
BstUI CGCG 4 cut(s) 50, 92, 320, 335
BstV1I GCAGC 8 cut(s) 27, 1075, 1278, 1642, 1804, 2445, 2845, 2864
BstV2I GAAGAC 4 cut(s) 296, 315, 1286, 2274
BstX2I RGATCY 2 cut(s) 678, 1951
BstYI RGATCY 2 cut(s) 678, 1951
BstZI CGGCCG 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 500
BsuI GTATCC 1 cut(s) 2715
BsuRI GGCC 6 cut(s) 262, 302, 343, 423, 1260, 2356
BsuTUI ATCGAT 1 cut(s) 500
BtgI CCRYGG 3 cut(s) 85, 695, 2325
BtgZI GCGATG 1 cut(s) 1428
BtrI CACGTC 1 cut(s) 313
BtsIMutI CAGTG 6 cut(s) 640, 663, 1346, 2076, 2257, 2680
BveI ACCTGC 3 cut(s) 1006, 1796, 2452
Cac8I GCNNGC 5 cut(s) 304, 1039, 1653, 2186, 2190
CaiI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2858
CciI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
CfoI GCGC 3 cut(s) 50, 66, 92
Cfr10I RCCGGY 2 cut(s) 271, 302
Cfr13I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2551
Cfr9I CCCGGG 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 500
CseI GACGC 2 cut(s) 326, 341
CsiI ACCWGGT 1 cut(s) 1303
Csp6I GTAC 7 cut(s) 190, 238, 379, 799, 1683, 2446, 2640
CviQI GTAC 7 cut(s) 190, 238, 379, 799, 1683, 2446, 2640
DdeI CTNAG 8 cut(s) 143, 847, 854, 1720, 1848, 2020, 2172, 2582
DraI TTTAAA 1 cut(s) 814
EaeI YGGCCR 4 cut(s) 260, 341, 421, 2354
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 2037
EarI CTCTTC 1 cut(s) 2037
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 3 cut(s) 1005, 2325, 2410
Eco24I GRGCYC 1 cut(s) 2180
Eco47I GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2551
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 5 cut(s) 1485, 1593, 1968, 2396, 2685
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 3 cut(s) 993, 1808, 2087
EcoO109I RGGNCCY 4 cut(s) 214, 397, 1070, 1259
EcoO65I GGTNACC 3 cut(s) 993, 1808, 2087
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 4 cut(s) 111, 1303, 1633, 2547
EcoT14I CCWWGG 3 cut(s) 1005, 2325, 2410
EcoT22I ATGCAT 4 cut(s) 1746, 1917, 2335, 2424
EcoT38I GRGCYC 1 cut(s) 2180
ErhI CCWWGG 3 cut(s) 1005, 2325, 2410
FalI AAGNNNNNCTT 2 cut(s) 519, 551
FaqI GGGAC 7 cut(s) 386, 410, 529, 1606, 2020, 2113, 2209
FauI CCCGC 1 cut(s) 408
FauNDI CATATG 1 cut(s) 864
FbaI TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2628
FblI GTMKAC 1 cut(s) 1309
FriOI GRGCYC 1 cut(s) 2180
FspAI RTGCGCAY 1 cut(s) 65
FspBI CTAG 5 cut(s) 1038, 2405, 2475, 2507, 2849
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 3 cut(s) 49, 65, 91
GsaI CCCAGC 5 cut(s) 1070, 2007, 2100, 2196, 2528
GsuI CTGGAG 2 cut(s) 134, 2531
HaeIII GGCC 6 cut(s) 262, 302, 343, 423, 1260, 2356
HgaI GACGC 2 cut(s) 326, 341
HhaI GCGC 3 cut(s) 50, 66, 92
Hin6I GCGC 3 cut(s) 48, 64, 90
HinP1I GCGC 3 cut(s) 48, 64, 90
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HphI GGTGA 4 cut(s) 364, 987, 1636, 2246
Hpy166II GTNNAC 7 cut(s) 240, 316, 457, 1310, 1343, 1683, 2448
Hpy8I GTNNAC 7 cut(s) 240, 316, 457, 1310, 1343, 1683, 2448
Hpy99I CGWCG 4 cut(s) 109, 127, 268, 335
HpyCH4III ACNGT 4 cut(s) 635, 658, 1630, 2080
HpyCH4IV ACGT 2 cut(s) 246, 312
HpyF3I CTNAG 8 cut(s) 143, 847, 854, 1720, 1848, 2020, 2172, 2582
HpySE526I ACGT 2 cut(s) 246, 312
HspAI GCGC 3 cut(s) 48, 64, 90
KflI GGGWCCC 1 cut(s) 397
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 5 cut(s) 1663, 1882, 2038, 2122, 2628
LmnI GCTCC 4 cut(s) 115, 286, 864, 1335
Lsp1109I GCAGC 8 cut(s) 27, 1075, 1278, 1642, 1804, 2445, 2845, 2864
LweI GCATC 8 cut(s) 727, 1132, 1613, 1902, 2144, 2320, 2674, 2835
MabI ACCWGGT 1 cut(s) 1303
MaeI CTAG 5 cut(s) 1038, 2405, 2475, 2507, 2849
MaeII ACGT 2 cut(s) 246, 312
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 2 cut(s) 678, 1951
MhlI GDGCHC 2 cut(s) 1721, 2180
MluI ACGCGT 2 cut(s) 318, 333
MlyI GAGTC 7 cut(s) 448, 511, 899, 1454, 2060, 2474, 2625
MmeI TCCRAC 8 cut(s) 706, 745, 1184, 1246, 1979, 2298, 2578, 2766
Mph1103I ATGCAT 4 cut(s) 1746, 1917, 2335, 2424
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 3 cut(s) 293, 836, 2817
MspA1I CMGCKG 4 cut(s) 229, 1066, 2435, 2858
MspCI CTTAAG 1 cut(s) 2597
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 4 cut(s) 1744, 1915, 2333, 2817
MvaI CCWGG 4 cut(s) 113, 1305, 1635, 2549
MvnI CGCG 4 cut(s) 50, 92, 320, 335
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 7 cut(s) 101, 285, 286, 345, 513, 1863, 2093
NcoI CCATGG 1 cut(s) 2325
NdeI CATATG 1 cut(s) 864
NgoMIV GCCGGC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 1037
NlaIV GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 860, 1071
NmuCI GTSAC 9 cut(s) 202, 440, 805, 842, 993, 1624, 2057, 2252, 2477
NsbI TGCGCA 1 cut(s) 65
NsiI ATGCAT 4 cut(s) 1746, 1917, 2335, 2424
NspI RCATGY 1 cut(s) 1424
NspV TTCGAA 1 cut(s) 2453
PagI TCATGA 4 cut(s) 1660, 1783, 1879, 2119
PaqCI CACCTGC 1 cut(s) 1006
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 4 cut(s) 1744, 1915, 2333, 2817
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 3 cut(s) 293, 836, 2817
PflFI GACNNNGTC 2 cut(s) 1285, 2615
PfoI TCCNGGA 1 cut(s) 111
PleI GAGTC 7 cut(s) 447, 511, 898, 1454, 2060, 2474, 2624
PpsI GAGTC 7 cut(s) 447, 511, 898, 1454, 2060, 2474, 2624
PpuMI RGGWCCY 3 cut(s) 214, 397, 1070
PshBI ATTAAT 1 cut(s) 1521
Psp5II RGGWCCY 3 cut(s) 214, 397, 1070
Psp6I CCWGG 4 cut(s) 111, 1303, 1633, 2547
PspEI GGTNACC 3 cut(s) 993, 1808, 2087
PspFI CCCAGC 5 cut(s) 1066, 2003, 2096, 2192, 2524
PspGI CCWGG 4 cut(s) 111, 1303, 1633, 2547
PspN4I GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 860, 1071
PspPI GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2551
PspPPI RGGWCCY 3 cut(s) 214, 397, 1070
PstI CTGCAG 1 cut(s) 2463
PstNI CAGNNNCTG 4 cut(s) 662, 1463, 1847, 2858
PsuI RGATCY 2 cut(s) 678, 1951
PsyI GACNNNGTC 2 cut(s) 1285, 2615
PvuII CAGCTG 2 cut(s) 1066, 2858
RsaI GTAC 7 cut(s) 191, 239, 380, 800, 1684, 2447, 2641
RsaNI GTAC 7 cut(s) 190, 238, 379, 799, 1683, 2446, 2640
Sau96I GGNCC 8 cut(s) 184, 214, 397, 468, 1070, 1126, 1259, 2551
SchI GAGTC 7 cut(s) 448, 511, 899, 1454, 2060, 2474, 2625
SduI GDGCHC 2 cut(s) 1721, 2180
SexAI ACCWGGT 1 cut(s) 1303
SfaNI GCATC 8 cut(s) 727, 1132, 1613, 1902, 2144, 2320, 2674, 2835
SfcI CTRYAG 2 cut(s) 654, 2459
SfuI TTCGAA 1 cut(s) 2453
SgrAI CRCCGGYG 1 cut(s) 271
SinI GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2551
SmaI CCCGGG 1 cut(s) 286
SmlI CTYRAG 3 cut(s) 1939, 1974, 2597
SmoI CTYRAG 3 cut(s) 1939, 1974, 2597
SspMI CTAG 5 cut(s) 1038, 2405, 2475, 2507, 2849
StyI CCWWGG 3 cut(s) 1005, 2325, 2410
TaaI ACNGT 4 cut(s) 635, 658, 1630, 2080
TaiI ACGT 2 cut(s) 249, 315
TaqI TCGA 8 cut(s) 125, 170, 297, 500, 902, 2442, 2453, 2829
TatI WGTACW 5 cut(s) 189, 237, 798, 1682, 2445
TauI GCSGC 5 cut(s) 121, 229, 271, 2435, 2762
TscAI CASTG 6 cut(s) 640, 663, 1353, 2083, 2257, 2680
TseFI GTSAC 9 cut(s) 202, 440, 805, 842, 993, 1624, 2057, 2252, 2477
TseI GCWGC 9 cut(s) 15, 1063, 1291, 1655, 1817, 2458, 2852, 2855, 2858
Tsp45I GTSAC 9 cut(s) 202, 440, 805, 842, 993, 1624, 2057, 2252, 2477
TspGWI ACGGA 3 cut(s) 221, 248, 2628
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 6 cut(s) 640, 663, 1353, 2083, 2257, 2680
Tth111I GACNNNGTC 2 cut(s) 1285, 2615
Vha464I CTTAAG 1 cut(s) 2597
VpaK11BI GGWCC 7 cut(s) 184, 214, 397, 468, 1070, 1126, 2551
VspI ATTAAT 1 cut(s) 1521
XapI RAATTY 5 cut(s) 836, 955, 1353, 1617, 1836
XceI RCATGY 1 cut(s) 1424
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1309
XmnI GAANNNNTTC 3 cut(s) 293, 836, 2817
XspI CTAG 5 cut(s) 1038, 2405, 2475, 2507, 2849
Zsp2I ATGCAT 4 cut(s) 1746, 1917, 2335, 2424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.