Rmu_ssc0000425.1_g000007

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000425.1
OG0000527
Physical Location & Seq
Forward (+)
52168 .. 56903
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000425.1_g000007.1.cds

Sequence Viewer

Length: 2370 bp
atggaggagcaggagagcaatggtggccataaccctgttgggtatttcacccaaggcgccaacgtcgaagtttgcagcaaagaaaaagggtctgaaggtgcatggttccctgcaacaatcttagagccaccagcaaactcctcagcctcaaaaagaaagacctttacttccaacaaggttttgattcggtacaagactcttgtctccgacaaggaccccaccaagccacttgtcgagcacaccaccttggggcttctccggccgccgcttccggctgacaatgcggaggaggagaggtttgaggagaacgacgtcgttgatgcttttcaccttgaaggttggtggactgggttggtgatgagtgttgtgggggagaagtatggtgtggggtttaagaaaccgccggattatctggagctggagcgcagtaagttgaggtcacattgggattgggaccatggggtttggaccagagccccaagacaggtgttgaggggatcaaattacaagccatggacaccagttgaagtgaatctggaaaaagatcatctatggtcttcgtggttacctgcattttttattggtcaagtagggggtgactctttcttggtgcaatacaaatataagaccccagaaaatgatgataaaaatggtcttgttaaagtagttgtagctgatcaccagattcgacctcgccctccacagcaagaggaaaaagattttcacttgtcacaaatggtggatgcattctgtgatatgggttggtgggtaggggacattacagaagttcttacagacaagaggtacgttgtcactttcagattcacaaaggaggagaaggagttttgtcatccagagttaaggtgccacttggaatggattggtaaacaatgggtgactagcgattcggaatttcattctactaagtattgcggagtacaaccaagacatgcacgtgaaatttctagtaattctgtaggcaaaacaccttgtcataccaagtttaggatgaagaaattgagtccttgcatggataagttacattgtgaaagaaccaaagacaaactgaaaatatctcagcatcctagcgatgattcaacatttttaagtcccactccctgcaagaagttaaaaggggatgattcagaagacaccttgtctcttgcacaatcctatcaaagcaaaaccccagctaaaacatcaaagaggaaaggcctatttactgatgaagagacgaatgagcaacagcagtttgaacaactggatagtctagcaatcaattttgtaaaaagggggagacaaccaaactcacaatttgtaaaacgtggaaggaagggtataccccgaaaattgcatgtcagcaatggcagtaaaagtgtaagaggtgaccaagaatacattgaagatgaaaatgtaatgaccaggattgaatcttttggtatcccgtcagagcctgaagcaattgggggcttgcatgctggaaattcatgtctgcttcctgtgaaggagattaaacatggtaatgaagataaagtgagcaggggaataaaacaggctgagttacttgttcaacacccaaaagatccagcagcaggttgtatgaatgatatacttgaaaagcctgcaaatccaagcgggaaccaaaacctggcaacttaccaaagaaagaaaaggttcaaactgacaggtgtaaagcataacggaactggagtttttactgatgctgaagtgtccaagactaatgttgttgaacatataggtaaccctgctgggactgtcaaccaacaagaaaaaagtctaaatctgccttttgtaaagaattctccactatgggaacatcttgaatccatggaagtattcaaaaactttccgcagaagccgcattttcattttgcggtgaacttgaatcctgtatttcgtgaaggagcagccattggcagtatgttagcctttgcttctttggttgagaagatttccaatttaggagttgatgatcctagagaattaatcgaaagctatttgaaaggactagttgaaatggaattgatgggatttgatgtcaaggcaccaaggcatcgtctgactgaattgctagacatgaaaataaagttgggacagcttcagaaacaatcaaaagaagttaaacttaggatcacagggagtactcatgacacaaccacctatgatgaaacaatcactgggattgacaagaagattaaggacttgcaagaaaaacggatgatggcagtgtcaatgaaagaggtcaaagtttctgaaattagcaggttgcaagcagaagcaacagctatcaatgaagacatccagagtatcagatgtgattttggaaacctagtggctgctgaatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

789

Amino Acids

89.42

Weight (kDa)

7.14

Isoelectric Point (pI)

38.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 3 cut(s) 577, 1583, 2274
AccB1I GGYRCC 3 cut(s) 56, 864, 2065
AccB7I CCANNNNNTGG 1 cut(s) 1648
AccI GTMKAC 1 cut(s) 1339
AciI CCGC 9 cut(s) 263, 266, 284, 401, 933, 1635, 1871, 1880, 1895
AclWI GGATC 4 cut(s) 505, 1577, 1988, 2159
AcoI YGGCCR 2 cut(s) 25, 260
AcsI RAATTY 4 cut(s) 911, 960, 1483, 1819
AcuI CTGAAG 4 cut(s) 114, 1476, 1746, 2105
AcvI CACGTG 1 cut(s) 956
AcyI GRCGYC 2 cut(s) 57, 312
AfaI GTAC 4 cut(s) 191, 806, 939, 2164
AfiI CCNNNNNNNGG 5 cut(s) 249, 484, 1005, 1592, 1648
AhdI GACNNNNNGTC 1 cut(s) 1156
AhlI ACTAGT 1 cut(s) 2029
AjnI CCWGG 2 cut(s) 1421, 1647
AjuI GAANNNNNNNTTGG 2 cut(s) 2093, 2125
AluBI AGCT 6 cut(s) 418, 674, 1193, 2016, 2119, 2306
AluI AGCT 6 cut(s) 418, 674, 1193, 2016, 2119, 2306
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 4 cut(s) 208, 1164, 1226, 1291
AlwI GGATC 4 cut(s) 505, 1577, 1988, 2159
AlwNI CAGNNNCTG 1 cut(s) 1454
AoxI GGCC 3 cut(s) 25, 260, 1213
ApeKI GCWGC 4 cut(s) 75, 1589, 1928, 2357
ApoI RAATTY 4 cut(s) 911, 960, 1483, 1819
ArsI GACNNNNNNTTYG 2 cut(s) 889, 921
AseI ATTAAT 1 cut(s) 2006
Asp700I GAANNNNTTC 1 cut(s) 1673
AspLEI GCGC 2 cut(s) 59, 426
AspS9I GGNCC 3 cut(s) 214, 454, 468
AsuHPI GGTGA 8 cut(s) 40, 320, 367, 608, 671, 907, 1397, 1909
AvaII GGWCC 3 cut(s) 214, 454, 468
BalI TGGCCA 1 cut(s) 27
BanI GGYRCC 3 cut(s) 56, 864, 2065
BanII GRGCYC 1 cut(s) 478
BbrPI CACGTG 1 cut(s) 956
BbsI GAAGAC 3 cut(s) 549, 1155, 2322
Bbv12I GWGCWC 1 cut(s) 240
BbvCI CCTCAGC 1 cut(s) 142
BbvI GCAGC 4 cut(s) 87, 1601, 1940, 2344
BccI CCATC 2 cut(s) 2041, 2236
BciT130I CCWGG 2 cut(s) 1423, 1649
BciVI GTATCC 1 cut(s) 1451
BclI TGATCA 1 cut(s) 676
BcoDI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BcuI ACTAGT 1 cut(s) 2029
BfaI CTAG 8 cut(s) 900, 966, 1086, 1271, 1998, 2030, 2093, 2351
BfmI CTRYAG 1 cut(s) 975
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 3 cut(s) 577, 1583, 2274
BfuI GTATCC 1 cut(s) 1451
BisI GCNGC 7 cut(s) 76, 263, 266, 1590, 1880, 1929, 2358
BlsI GCNGC 7 cut(s) 77, 264, 267, 1591, 1881, 1930, 2359
BmcAI AGTACT 1 cut(s) 2164
Bme1390I CCNGG 2 cut(s) 1423, 1649
Bme18I GGWCC 3 cut(s) 214, 454, 468
BmeRI GACNNNNNGTC 1 cut(s) 1156
BmgT120I GGNCC 3 cut(s) 214, 454, 468
BmiI GGNNCC 7 cut(s) 58, 107, 216, 455, 866, 1640, 2067
BmrFI CCNGG 2 cut(s) 1423, 1649
BmrI ACTGGG 2 cut(s) 357, 2208
BmsI GCATC 5 cut(s) 310, 733, 1090, 1711, 2083
BmuI ACTGGG 2 cut(s) 357, 2208
BoxI GACNNNNGTC 1 cut(s) 200
BpiI GAAGAC 3 cut(s) 549, 1155, 2322
BpmI CTGGAG 3 cut(s) 434, 440, 1728
Bpu10I CCTNAGC 1 cut(s) 142
BsaAI YACGTR 1 cut(s) 956
BsaHI GRCGYC 2 cut(s) 57, 312
BsaJI CCNNGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 827, 857
Bsc4I CCNNNNNNNGG 5 cut(s) 249, 484, 1005, 1592, 1648
Bse1I ACTGG 5 cut(s) 352, 521, 1266, 1711, 2203
Bse3DI GCAATG 2 cut(s) 25, 1369
BseBI CCWGG 2 cut(s) 1423, 1649
BseDI CCNNGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
BseGI GGATG 7 cut(s) 748, 850, 1014, 1081, 1144, 2244, 2319
BseLI CCNNNNNNNGG 5 cut(s) 249, 484, 1005, 1592, 1648
BseMI GCAATG 2 cut(s) 25, 1369
BseMII CTCAG 3 cut(s) 156, 1091, 1548
BseNI ACTGG 5 cut(s) 352, 521, 1266, 1711, 2203
BseRI GAGGAG 6 cut(s) 20, 130, 302, 305, 317, 848
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 4 cut(s) 87, 1601, 1940, 2344
BseYI CCCAGC 2 cut(s) 1189, 1769
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 3 cut(s) 27, 262, 1215
BshNI GGYRCC 3 cut(s) 56, 864, 2065
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 3 cut(s) 259, 272, 404
BslFI GGGAC 5 cut(s) 467, 788, 1095, 1786, 2127
BslI CCNNNNNNNGG 5 cut(s) 249, 484, 1005, 1592, 1648
BsmAI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BsmBI CGTCTC 1 cut(s) 1226
BsmFI GGGAC 5 cut(s) 467, 788, 1095, 1786, 2127
BsmI GAATGC 1 cut(s) 746
BsnI GGCC 3 cut(s) 27, 262, 1215
Bsp1286I GDGCHC 2 cut(s) 240, 478
Bsp143I GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
Bsp19I CCATGG 3 cut(s) 457, 512, 1848
BspACI CCGC 9 cut(s) 263, 266, 284, 401, 933, 1635, 1871, 1880, 1895
BspANI GGCC 3 cut(s) 27, 262, 1215
BspCNI CTCAG 3 cut(s) 155, 1090, 1549
BspHI TCATGA 1 cut(s) 2167
BspLI GGNNCC 7 cut(s) 58, 107, 216, 455, 866, 1640, 2067
BspMI ACCTGC 3 cut(s) 577, 1583, 2274
BspPI GGATC 4 cut(s) 505, 1577, 1988, 2159
BspT107I GGYRCC 3 cut(s) 56, 864, 2065
BsrDI GCAATG 2 cut(s) 25, 1369
BsrI ACTGG 5 cut(s) 352, 521, 1266, 1711, 2203
BssECI CCNNGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
BssMI GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
BssNAI GTATAC 1 cut(s) 1340
BssNI GRCGYC 2 cut(s) 57, 312
BssT1I CCWWGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
Bst1107I GTATAC 1 cut(s) 1340
Bst2UI CCWGG 2 cut(s) 1423, 1649
Bst4CI ACNGT 1 cut(s) 1777
Bst6I CTCTTC 1 cut(s) 1224
BstACI GRCGYC 2 cut(s) 57, 312
BstBAI YACGTR 1 cut(s) 956
BstC8I GCNNGC 4 cut(s) 1472, 1476, 1623, 2292
BstDEI CTNAG 6 cut(s) 121, 142, 924, 1077, 1557, 2147
BstDSI CCRYGG 3 cut(s) 457, 512, 1848
BstEII GGTNACC 3 cut(s) 564, 1385, 1760
BstF5I GGATG 7 cut(s) 748, 850, 1014, 1081, 1144, 2244, 2319
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 426
BstKTI GATC 6 cut(s) 500, 547, 679, 1585, 1996, 2154
BstMAI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BstMBI GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1879
BstNI CCWGG 2 cut(s) 1423, 1649
BstNSI RCATGY 3 cut(s) 953, 1358, 1478
BstPAI GACNNNNGTC 1 cut(s) 200
BstPI GGTNACC 3 cut(s) 564, 1385, 1760
BstSCI CCNGG 2 cut(s) 1421, 1647
BstSFI CTRYAG 1 cut(s) 975
BstV1I GCAGC 4 cut(s) 87, 1601, 1940, 2344
BstV2I GAAGAC 3 cut(s) 549, 1155, 2322
BstX2I RGATCY 1 cut(s) 1582
BstYI RGATCY 1 cut(s) 1582
BstZ17I GTATAC 1 cut(s) 1340
BstZI CGGCCG 1 cut(s) 260
BsuI GTATCC 1 cut(s) 1451
BsuRI GGCC 3 cut(s) 27, 262, 1215
BtgI CCRYGG 3 cut(s) 457, 512, 1848
BtgZI GCGATG 1 cut(s) 1104
BtsCI GGATG 7 cut(s) 748, 850, 1014, 1081, 1144, 2244, 2319
BtsI GCAGTG 1 cut(s) 2253
BtsIMutI CAGTG 2 cut(s) 2196, 2253
BveI ACCTGC 3 cut(s) 577, 1583, 2274
Cac8I GCNNGC 4 cut(s) 1472, 1476, 1623, 2292
CaiI CAGNNNCTG 1 cut(s) 1454
CciI TCATGA 1 cut(s) 2167
CfoI GCGC 2 cut(s) 59, 426
Cfr13I GGNCC 3 cut(s) 214, 454, 468
Csp6I GTAC 4 cut(s) 190, 805, 938, 2163
CviQI GTAC 4 cut(s) 190, 805, 938, 2163
DdeI CTNAG 6 cut(s) 121, 142, 924, 1077, 1557, 2147
DinI GGCGCC 1 cut(s) 58
DpnI GATC 6 cut(s) 499, 546, 678, 1584, 1995, 2153
DpnII GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
DriI GACNNNNNGTC 1 cut(s) 1156
EaeI YGGCCR 2 cut(s) 25, 260
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 1224
Eam1105I GACNNNNNGTC 1 cut(s) 1156
EarI CTCTTC 1 cut(s) 1224
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 214, 454, 468
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 4 cut(s) 114, 1476, 1746, 2105
Eco72I CACGTG 1 cut(s) 956
Eco91I GGTNACC 3 cut(s) 564, 1385, 1760
EcoO109I RGGNCCY 1 cut(s) 214
EcoO65I GGTNACC 3 cut(s) 564, 1385, 1760
EcoRI GAATTC 1 cut(s) 1819
EcoRII CCWGG 2 cut(s) 1421, 1647
EcoT14I CCWWGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
Esp3I CGTCTC 1 cut(s) 1226
FalI AAGNNNNNCTT 2 cut(s) 2130, 2162
FaqI GGGAC 5 cut(s) 467, 788, 1095, 1786, 2127
FauI CCCGC 1 cut(s) 1628
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 1339
Fnu4HI GCNGC 7 cut(s) 76, 263, 266, 1590, 1880, 1929, 2358
FokI GGATG 7 cut(s) 755, 837, 1021, 1068, 1151, 2251, 2306
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 7 cut(s) 76, 263, 266, 1590, 1880, 1929, 2358
FspBI CTAG 8 cut(s) 900, 966, 1086, 1271, 1998, 2030, 2093, 2351
GlaI GCGC 2 cut(s) 58, 425
GluI GCNGC 7 cut(s) 76, 263, 266, 1590, 1880, 1929, 2358
GsaI CCCAGC 2 cut(s) 1193, 1773
GsuI CTGGAG 3 cut(s) 434, 440, 1728
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 27, 262, 1215
HapII CCGG 3 cut(s) 259, 272, 404
HhaI GCGC 2 cut(s) 59, 426
Hin1I GRCGYC 2 cut(s) 57, 312
Hin6I GCGC 2 cut(s) 57, 424
HinP1I GCGC 2 cut(s) 57, 424
HincII GTYRAC 1 cut(s) 1780
HindII GTYRAC 1 cut(s) 1780
HpaII CCGG 3 cut(s) 259, 272, 404
HphI GGTGA 8 cut(s) 40, 320, 367, 608, 671, 907, 1397, 1909
Hpy166II GTNNAC 5 cut(s) 345, 887, 1340, 1780, 1900
Hpy188III TCNNGA 7 cut(s) 413, 536, 854, 1841, 1919, 2168, 2323
Hpy8I GTNNAC 5 cut(s) 345, 887, 1340, 1780, 1900
Hpy99I CGWCG 3 cut(s) 68, 314, 317
HpyAV CCTTC 7 cut(s) 89, 329, 832, 1323, 1327, 1498, 1916
HpyCH4III ACNGT 1 cut(s) 1777
HpyCH4IV ACGT 5 cut(s) 63, 312, 807, 955, 1324
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1879
HpyF3I CTNAG 6 cut(s) 121, 142, 924, 1077, 1557, 2147
HpySE526I ACGT 5 cut(s) 63, 312, 807, 955, 1324
Hsp92I GRCGYC 2 cut(s) 57, 312
HspAI GCGC 2 cut(s) 57, 424
KasI GGCGCC 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
LmnI GCTCC 4 cut(s) 7, 415, 421, 1925
Lsp1109I GCAGC 4 cut(s) 87, 1601, 1940, 2344
LweI GCATC 5 cut(s) 310, 733, 1090, 1711, 2083
MaeI CTAG 8 cut(s) 900, 966, 1086, 1271, 1998, 2030, 2093, 2351
MaeII ACGT 5 cut(s) 63, 312, 807, 955, 1324
MalI GATC 6 cut(s) 499, 546, 678, 1584, 1995, 2153
MboI GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
MboII GAAGA 9 cut(s) 549, 1024, 1160, 1241, 1415, 1538, 1981, 2224, 2327
MfeI CAATTG 1 cut(s) 1461
MflI RGATCY 1 cut(s) 1582
MhlI GDGCHC 2 cut(s) 240, 478
MlsI TGGCCA 1 cut(s) 27
MluNI TGGCCA 1 cut(s) 27
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 3 cut(s) 190, 593, 1030
MmeI TCCRAC 2 cut(s) 195, 231
Mox20I TGGCCA 1 cut(s) 27
Mph1103I ATGCAT 1 cut(s) 748
MroXI GAANNNNTTC 1 cut(s) 1673
MscI TGGCCA 1 cut(s) 27
MseI TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2006, 2142, 2217
MslI CAYNNNNRTG 2 cut(s) 954, 1521
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 3 cut(s) 259, 272, 404
MspR9I CCNGG 2 cut(s) 1423, 1649
MunI CAATTG 1 cut(s) 1461
Mva1269I GAATGC 1 cut(s) 746
MvaI CCWGG 2 cut(s) 1423, 1649
MwoI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1879
NarI GGCGCC 1 cut(s) 57
NcoI CCATGG 3 cut(s) 457, 512, 1848
NdeII GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
NlaIV GGNNCC 7 cut(s) 58, 107, 216, 455, 866, 1640, 2067
NmuCI GTSAC 6 cut(s) 438, 596, 729, 811, 895, 1385
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 3 cut(s) 953, 1358, 1478
PaeI GCATGC 1 cut(s) 1478
PagI TCATGA 1 cut(s) 2167
PceI AGGCCT 1 cut(s) 1215
PctI GAATGC 1 cut(s) 746
PdmI GAANNNNTTC 1 cut(s) 1673
PflMI CCANNNNNTGG 1 cut(s) 1648
PkrI GCNGC 7 cut(s) 77, 264, 267, 1591, 1881, 1930, 2359
PleI GAGTC 3 cut(s) 190, 593, 1029
PluTI GGCGCC 1 cut(s) 60
PmaCI CACGTG 1 cut(s) 956
PmlI CACGTG 1 cut(s) 956
PpsI GAGTC 3 cut(s) 190, 593, 1029
Ppu21I YACGTR 1 cut(s) 956
PpuMI RGGWCCY 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 200
PshBI ATTAAT 1 cut(s) 2006
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 1421, 1647
PspCI CACGTG 1 cut(s) 956
PspEI GGTNACC 3 cut(s) 564, 1385, 1760
PspFI CCCAGC 2 cut(s) 1189, 1769
PspGI CCWGG 2 cut(s) 1421, 1647
PspN4I GGNNCC 7 cut(s) 58, 107, 216, 455, 866, 1640, 2067
PspPI GGNCC 3 cut(s) 214, 454, 468
PspPPI RGGWCCY 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 1454
PsuI RGATCY 1 cut(s) 1582
RsaI GTAC 4 cut(s) 191, 806, 939, 2164
RsaNI GTAC 4 cut(s) 190, 805, 938, 2163
RseI CAYNNNNRTG 2 cut(s) 954, 1521
SaqAI TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2006, 2142, 2217
SatI GCNGC 7 cut(s) 76, 263, 266, 1590, 1880, 1929, 2358
Sau3AI GATC 6 cut(s) 497, 544, 676, 1582, 1993, 2151
Sau96I GGNCC 3 cut(s) 214, 454, 468
ScaI AGTACT 1 cut(s) 2164
SchI GAGTC 3 cut(s) 190, 593, 1030
ScrFI CCNGG 2 cut(s) 1423, 1649
SduI GDGCHC 2 cut(s) 240, 478
SfaNI GCATC 5 cut(s) 310, 733, 1090, 1711, 2083
SfcI CTRYAG 1 cut(s) 975
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 3 cut(s) 214, 454, 468
SmiMI CAYNNNNRTG 2 cut(s) 954, 1521
SpeI ACTAGT 1 cut(s) 2029
SphI GCATGC 1 cut(s) 1478
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 9 cut(s) 263, 266, 284, 401, 933, 1635, 1871, 1880, 1895
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 8 cut(s) 900, 966, 1086, 1271, 1998, 2030, 2093, 2351
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 2 cut(s) 1421, 1647
StyI CCWWGG 6 cut(s) 52, 246, 457, 512, 1848, 2069
TaaI ACNGT 1 cut(s) 1777
TaiI ACGT 5 cut(s) 66, 315, 810, 958, 1327
TaqI TCGA 4 cut(s) 66, 234, 688, 2010
TatI WGTACW 2 cut(s) 937, 2162
TauI GCSGC 3 cut(s) 265, 268, 1882
Tru1I TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2006, 2142, 2217
Tru9I TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2006, 2142, 2217
TscAI CASTG 2 cut(s) 2203, 2253
TseFI GTSAC 6 cut(s) 438, 596, 729, 811, 895, 1385
TseI GCWGC 4 cut(s) 75, 1589, 1928, 2357
Tsp45I GTSAC 6 cut(s) 438, 596, 729, 811, 895, 1385
TspGWI ACGGA 2 cut(s) 1716, 2251
TspRI CASTG 2 cut(s) 2203, 2253
Van91I CCANNNNNTGG 1 cut(s) 1648
VpaK11BI GGWCC 3 cut(s) 214, 454, 468
VspI ATTAAT 1 cut(s) 2006
XapI RAATTY 4 cut(s) 911, 960, 1483, 1819
XceI RCATGY 3 cut(s) 953, 1358, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 1339
XmnI GAANNNNTTC 1 cut(s) 1673
XspI CTAG 8 cut(s) 900, 966, 1086, 1271, 1998, 2030, 2093, 2351
ZraI GACGTC 1 cut(s) 313
ZrmI AGTACT 1 cut(s) 2164
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.