Prupe.2G269100_v2.0.a1

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000527
Physical Location & Seq
Reverse (-)
27398173 .. 27404268
6096 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G269100.8

Sequence Viewer

Length: 2427 bp
ATGGTGGTGAAGCGGAGCCAAGCGCATTTGAGCAAGGATTCAAAGGTGGAAGTTTGCAGCAACGAAGACGGGTACCAAGGTGCATGGTTCCCAGCCATATTCCTTGACCCACAACCCTCAGATGGCACACCAAAGAAGAAAAGGAAGAGCCTTGGCAACTCAAGCAAGGCTCTGGTGCAGTACGAGACTCTGGTCTCCGACGATGACCCAAACAAGCCACTCACTGAGCTCGTTGACGTGTGCTCCATCCGGCCAGTGCCTCCTCCTGACAATCCTGACCAGCCCTTCGAGCCCGCAAACGTCGTAGACGCCTTTTACCAAGAAGCTTGGTGGGTGGGGGTTGTGATAAGGTTTGAGGATGACAAGTATACGGTTGGTTTCAAAAACCCACCCGATCTGCTTGAGTTAAGGCGCTCTGAGCTGCGGCCTCATTGGGATTTTCTGGACGGCGTATGGGTTCGAGCCCGGAAGGAGAGGATGGTGGGATCATTTTTCAGCCCTGGGACAGCAGTGGAAGTGAATCTCTACAAAGAACATCTATTCTGTGCTTGGTTCCCAGCAATTTATCTTGGTGAACTAGGGGCCAACAATTTCTTGCTTCAATATAAGAGCTCAAACAACTGTGATGTTAAAGCGGTCGTAGGTGGCAAACAGATTCGGCCTCAGCCTCCAAAGTTGGCTGAAAGAGATTTTAACTTGATGGAAAAGGTGGATGCATTCTTTGATATGGGTTGGTGGGTCGGAGAGATTATAAAAGTTCTTACAGGAAAGAAGTATATGGTCTGTTTAAAATTGACAGAGGAGGTGAAGCAATACAGTCAGTCAGAATTGAGGCGTCACATGTACTGGACTGATGGAAGATGGGTTACTATGATCAATGGAAAACGGGTTACTATGATCAATAGAAACTGGGTTACTATGATCAATGGAAACTGGGTTCCTTACTTTAAGGAAGGTCACTTTAGCAGGGATTATGAAGTACGATCAAAGCGTGCATGCGAGAGTTCCAGGAGTTCTGAGTTAGCTGCAGCACTTGAATGTACAGGTGCTACCAAGGATAATAATGAAGAAAAAACACCTTGTTTGAGGATTTCGTGGAAGAATCAGTCGGAGGACTTGAGTCCTTGCATTGATAAATCACCTTATGGAAAGACCAAAAAGAAAATTAAAATTAATCAGAAACCTAACGATGATGCAACCATTTTAGGTCTTTCCAAGAAGTTAATATGGGGACATTCAGAAGACTCCTTATCTTTTGCACAATTATTTCGAAGGAGCAAAGAAGCCCCAGTTAGAGATGGTGTGACTAAGCAACAGCAGGTTGGAGGACTGGACAATCAAGCAATAGTTTCTCTTAAACGAAAAGTCAAGAAGGGCAGTGAAATAAGAAAGGCTGATGAAGAAGATGTTGATGATGAGTATGGAGTAGACAGCATTGAATCTTCTGGTACAGAGTCTAAACTAACAGGGGGATCTCATGCTGACGCATCATGTCTGCTCCCCATGGAGGAGGTTGAATGGAATGAAGATGTAGCGAGCAGTGAATTGCATGTGATAAAAAGGTTGGAGTGGACAGGTGAGAAGCACAATGGAACTGGAGTTGTTGCTCAAGTTGAATTGACTAAGACTCAAGTTGCGAATGATAATGGTTCTGAAGAAGCGATGAGAGAGACGGAAACTGTGGATTCCGCAATGGATTACTTGACAAAAGAAGTTCAGGTGACTGTGACTGGAGTATCTGCAACAGCATCTGCCCATGATCAACCTTTATCACTGTATGTAGATGAAATGCATTCTATAAAAGCCAAAGAGTGCTCAAGTAACCCTGCTGGGACTGCCAAGCAACAAGAGAGTCCACATTCGCCTTTTGTGAGTTGTTCTCCATTTTGGAAAAGTATTGAATCCATGGAAGTATTCAAAAGATTTCCGCAAAGGCCACATTTTCTTCCTCTGATGAAGTGTAAAGCGGTTTGTCGCGAAGGATCCGCTCTTGGAAATATGATAACCTTTGCCTCTTTGGTGGAGAAGACTTCCAAGTTGCAAGTTGGCGATCCTATAGACTTATTTGATAGCAATTTGGAGGCACTGGTTGACTTGGAAATGTTGGGATTTGATGTCACGGCAGTACGGCATCGACTGAAGGAATTGATTGAAATGAAAGTTAAGTTGGGTCAGCTTGAGAACCAATCAAAAGAAGTTGACATTCAGATCACGGAGTGTACTTTTGACAGAACCAGAAACAATGAAACAATCAGTCGGATTGATAAGGAGATCAAGGACTTGAAGGAAAAACGGGGGACCCTGATGTCAATCGATGTGGCCAAAGGTTCTGAAATTAGCAAGCTGCAATCAGAAGCAAATGCTATAACTGAAGGCATTCAGAGCATCCATCGTGATTTTGAGAAATTAGCTGCTGCGGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

809

Amino Acids

91.14

Weight (kDa)

5.79

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 752
AasI GACNNNNNNGTC 1 cut(s) 2305
Acc36I ACCTGC 1 cut(s) 1309
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 72
AccBSI CCGCTC 1 cut(s) 1988
AccI GTMKAC 3 cut(s) 306, 368, 1428
AccII CGCG 1 cut(s) 1977
AciI CCGC 9 cut(s) 13, 294, 424, 635, 1689, 1928, 1967, 1986, 2417
AclWI GGATC 5 cut(s) 493, 1480, 1977, 1990, 2045
AcoI YGGCCR 2 cut(s) 251, 2319
AcuI CTGAAG 3 cut(s) 1674, 2159, 2391
AcyI GRCGYC 2 cut(s) 309, 835
AdeI CACNNNGTG 1 cut(s) 2217
AfaI GTAC 8 cut(s) 74, 182, 845, 981, 1042, 1450, 2127, 2221
AfiI CCNNNNNNNGG 2 cut(s) 122, 1507
AflIII ACRYGT 2 cut(s) 237, 840
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 2 cut(s) 499, 1007
AjuI GAANNNNNNNTTGG 2 cut(s) 2150, 2182
AloI GAACNNNNNNTCC 2 cut(s) 921, 953
AluBI AGCT 8 cut(s) 229, 326, 421, 612, 1025, 2176, 2344, 2411
AluI AGCT 8 cut(s) 229, 326, 421, 612, 1025, 2176, 2344, 2411
Alw21I GWGCWC 4 cut(s) 231, 245, 614, 1817
Alw26I GTCTC 3 cut(s) 179, 199, 1664
AlwI GGATC 5 cut(s) 493, 1480, 1977, 1990, 2045
AlwNI CAGNNNCTG 1 cut(s) 1751
AoxI GGCC 6 cut(s) 251, 425, 582, 659, 1934, 2319
ApeKI GCWGC 7 cut(s) 57, 421, 1025, 1028, 2344, 2411, 2414
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 1173
Asp700I GAANNNNTTC 1 cut(s) 2376
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 25, 414
AspS9I GGNCC 2 cut(s) 582, 2298
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 6 cut(s) 19, 584, 817, 1131, 1589, 1732
AsuII TTCGAA 1 cut(s) 1270
AvaII GGWCC 1 cut(s) 2298
BaeI ACNNNNGTAYC 4 cut(s) 64, 97, 1719, 1752
BalI TGGCCA 1 cut(s) 2321
BamHI GGATCC 1 cut(s) 1982
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 4 cut(s) 231, 294, 466, 614
BarI GAAGNNNNNNTAC 2 cut(s) 850, 882
BbsI GAAGAC 3 cut(s) 72, 1248, 2033
Bbv12I GWGCWC 4 cut(s) 231, 245, 614, 1817
BbvCI CCTCAGC 1 cut(s) 663
BbvI GCAGC 7 cut(s) 69, 408, 1012, 1040, 2331, 2398, 2401
BccI CCATC 8 cut(s) 116, 254, 472, 694, 848, 855, 1292, 2397
BceAI ACGGC 3 cut(s) 463, 2136, 2144
BciT130I CCWGG 2 cut(s) 501, 1009
BclI TGATCA 4 cut(s) 873, 897, 921, 1759
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 179, 199, 1664
BfaI CTAG 1 cut(s) 578
BfmI CTRYAG 2 cut(s) 1026, 2055
BfoI RGCGCY 1 cut(s) 415
BfuAI ACCTGC 1 cut(s) 1309
BisI GCNGC 9 cut(s) 58, 422, 425, 1026, 1029, 2345, 2412, 2415, 2418
BlsI GCNGC 9 cut(s) 59, 423, 426, 1027, 1030, 2346, 2413, 2416, 2419
Bme1390I CCNGG 3 cut(s) 466, 501, 1009
Bme18I GGWCC 1 cut(s) 2298
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 582, 2298
BmiI GGNNCC 9 cut(s) 17, 74, 89, 554, 583, 939, 1984, 2299, 2300
BmrFI CCNGG 3 cut(s) 466, 501, 1009
BmrI ACTGGG 3 cut(s) 919, 943, 1283
BmsI GCATC 6 cut(s) 703, 1183, 1496, 1757, 2140, 2394
BmuI ACTGGG 3 cut(s) 919, 943, 1283
BoxI GACNNNNGTC 2 cut(s) 191, 1119
BpiI GAAGAC 3 cut(s) 72, 1248, 2033
BplI GAGNNNNNCTC 2 cut(s) 1864, 1896
BpmI CTGGAG 2 cut(s) 1617, 1752
Bpu10I CCTNAGC 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 1270
BpuEI CTTGAG 7 cut(s) 145, 422, 1138, 1593, 1614, 1801, 2198
BpuMI CCSGG 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 2313
BsaBI GATNNNNATC 1 cut(s) 2309
BsaHI GRCGYC 2 cut(s) 309, 835
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 7 cut(s) 76, 151, 499, 500, 1053, 1503, 1905
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 1507
Bse1I ACTGG 9 cut(s) 254, 851, 914, 938, 1289, 1335, 1600, 1735, 2091
Bse3DI GCAATG 1 cut(s) 1698
Bse8I GATNNNNATC 1 cut(s) 2309
BseBI CCWGG 2 cut(s) 501, 1009
BseCI ATCGAT 1 cut(s) 2313
BseDI CCNNGG 7 cut(s) 76, 151, 499, 500, 1053, 1503, 1905
BseGI GGATG 5 cut(s) 246, 364, 483, 718, 2385
BseJI GATNNNNATC 1 cut(s) 2309
BseLI CCNNNNNNNGG 2 cut(s) 122, 1507
BseMI GCAATG 1 cut(s) 1698
BseMII CTCAG 5 cut(s) 132, 216, 408, 677, 1008
BseNI ACTGG 9 cut(s) 254, 851, 914, 938, 1289, 1335, 1600, 1735, 2091
BseRI GAGGAG 3 cut(s) 252, 815, 1523
BseXI GCAGC 7 cut(s) 69, 408, 1012, 1040, 2331, 2398, 2401
BseYI CCCAGC 3 cut(s) 91, 556, 1829
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 1977
Bsh1285I CGRYCG 1 cut(s) 639
BshFI GGCC 6 cut(s) 253, 427, 584, 661, 1936, 2321
BshNI GGYRCC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 2313
BsiEI CGRYCG 1 cut(s) 639
BsiHKAI GWGCWC 4 cut(s) 231, 245, 614, 1817
BsiSI CCGG 2 cut(s) 250, 466
BslFI GGGAC 4 cut(s) 517, 1245, 1846, 2311
BslI CCNNNNNNNGG 2 cut(s) 122, 1507
BsmAI GTCTC 3 cut(s) 179, 199, 1664
BsmBI CGTCTC 1 cut(s) 1664
BsmFI GGGAC 4 cut(s) 517, 1245, 1846, 2311
BsmI GAATGC 3 cut(s) 716, 1792, 2376
BsnI GGCC 6 cut(s) 253, 427, 584, 661, 1936, 2321
Bso31I GGTCTC 1 cut(s) 199
Bsp119I TTCGAA 1 cut(s) 1270
Bsp1286I GDGCHC 6 cut(s) 231, 245, 294, 466, 614, 1817
Bsp1407I TGTACA 1 cut(s) 1040
Bsp19I CCATGG 2 cut(s) 1503, 1905
Bsp68I TCGCGA 1 cut(s) 1977
BspACI CCGC 9 cut(s) 13, 294, 424, 635, 1689, 1928, 1967, 1986, 2417
BspANI GGCC 6 cut(s) 253, 427, 584, 661, 1936, 2321
BspCNI CTCAG 5 cut(s) 131, 217, 409, 676, 1009
BspDI ATCGAT 1 cut(s) 2313
BspFNI CGCG 1 cut(s) 1977
BspLI GGNNCC 9 cut(s) 17, 74, 89, 554, 583, 939, 1984, 2299, 2300
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 1 cut(s) 1309
BspPI GGATC 5 cut(s) 493, 1480, 1977, 1990, 2045
BspQI GCTCTTC 1 cut(s) 140
BspT104I TTCGAA 1 cut(s) 1270
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 199
BsrBI CCGCTC 1 cut(s) 1988
BsrDI GCAATG 1 cut(s) 1698
BsrGI TGTACA 1 cut(s) 1040
BsrI ACTGG 9 cut(s) 254, 851, 914, 938, 1289, 1335, 1600, 1735, 2091
BssECI CCNNGG 7 cut(s) 76, 151, 499, 500, 1053, 1503, 1905
BssNAI GTATAC 1 cut(s) 369
BssNI GRCGYC 2 cut(s) 309, 835
BssT1I CCWWGG 5 cut(s) 76, 151, 1053, 1503, 1905
Bst1107I GTATAC 1 cut(s) 369
Bst2UI CCWGG 2 cut(s) 501, 1009
Bst4CI ACNGT 6 cut(s) 373, 623, 818, 1681, 1726, 1776
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 2 cut(s) 309, 835
BstAUI TGTACA 1 cut(s) 1040
BstBI TTCGAA 1 cut(s) 1270
BstC8I GCNNGC 5 cut(s) 294, 993, 997, 1537, 2342
BstDEI CTNAG 7 cut(s) 118, 225, 417, 663, 1017, 1308, 1623
BstDSI CCRYGG 2 cut(s) 1503, 1905
BstF5I GGATG 5 cut(s) 246, 364, 483, 718, 2385
BstFNI CGCG 1 cut(s) 1977
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 2 cut(s) 25, 414
BstMAI GTCTC 3 cut(s) 179, 199, 1664
BstMCI CGRYCG 1 cut(s) 639
BstMWI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 1835, 2382, 2417
BstNI CCWGG 2 cut(s) 501, 1009
BstNSI RCATGY 3 cut(s) 844, 999, 1553
BstPAI GACNNNNGTC 2 cut(s) 191, 1119
BstSCI CCNGG 3 cut(s) 464, 499, 1007
BstSFI CTRYAG 2 cut(s) 1026, 2055
BstUI CGCG 1 cut(s) 1977
BstV1I GCAGC 7 cut(s) 69, 408, 1012, 1040, 2331, 2398, 2401
BstV2I GAAGAC 3 cut(s) 72, 1248, 2033
BstX2I RGATCY 2 cut(s) 1472, 1982
BstYI RGATCY 2 cut(s) 1472, 1982
BstZ17I GTATAC 1 cut(s) 369
Bsu15I ATCGAT 1 cut(s) 2313
BsuRI GGCC 6 cut(s) 253, 427, 584, 661, 1936, 2321
BsuTUI ATCGAT 1 cut(s) 2313
BtgI CCRYGG 2 cut(s) 1503, 1905
BtgZI GCGATG 1 cut(s) 1676
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 5 cut(s) 246, 364, 483, 718, 2385
BtsI GCAGTG 3 cut(s) 516, 1384, 1546
BtsIMutI CAGTG 7 cut(s) 222, 261, 516, 1384, 1546, 1772, 2084
BtuMI TCGCGA 1 cut(s) 1977
BveI ACCTGC 1 cut(s) 1309
Cac8I GCNNGC 5 cut(s) 294, 993, 997, 1537, 2342
CaiI CAGNNNCTG 1 cut(s) 1751
CfoI GCGC 2 cut(s) 25, 414
Cfr13I GGNCC 2 cut(s) 582, 2298
ClaI ATCGAT 1 cut(s) 2313
CseI GACGC 3 cut(s) 317, 824, 1493
Csp6I GTAC 8 cut(s) 73, 181, 844, 980, 1041, 1449, 2126, 2220
CspCI CAANNNNNGTGG 2 cut(s) 2298, 2333
CviQI GTAC 8 cut(s) 73, 181, 844, 980, 1041, 1449, 2126, 2220
DdeI CTNAG 7 cut(s) 118, 225, 417, 663, 1017, 1308, 1623
DraI TTTAAA 1 cut(s) 789
DraIII CACNNNGTG 1 cut(s) 2217
DrdI GACNNNNNNGTC 1 cut(s) 2305
DseDI GACNNNNNNGTC 1 cut(s) 2305
EaeI YGGCCR 2 cut(s) 251, 2319
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Ecl136II GAGCTC 2 cut(s) 229, 612
Eco130I CCWWGG 5 cut(s) 76, 151, 1053, 1503, 1905
Eco24I GRGCYC 4 cut(s) 231, 294, 466, 614
Eco31I GGTCTC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 2298
Eco53kI GAGCTC 2 cut(s) 229, 612
Eco57I CTGAAG 3 cut(s) 1674, 2159, 2391
EcoICRI GAGCTC 2 cut(s) 229, 612
EcoO109I RGGNCCY 1 cut(s) 2298
EcoRII CCWGG 2 cut(s) 499, 1007
EcoT14I CCWWGG 5 cut(s) 76, 151, 1053, 1503, 1905
EcoT22I ATGCAT 2 cut(s) 718, 1794
EcoT38I GRGCYC 4 cut(s) 231, 294, 466, 614
ErhI CCWWGG 5 cut(s) 76, 151, 1053, 1503, 1905
Esp3I CGTCTC 1 cut(s) 1664
FaqI GGGAC 4 cut(s) 517, 1245, 1846, 2311
FauI CCCGC 1 cut(s) 301
FbaI TGATCA 4 cut(s) 873, 897, 921, 1759
FblI GTMKAC 3 cut(s) 306, 368, 1428
Fnu4HI GCNGC 9 cut(s) 58, 422, 425, 1026, 1029, 2345, 2412, 2415, 2418
FokI GGATG 5 cut(s) 233, 371, 490, 725, 2372
FriOI GRGCYC 4 cut(s) 231, 294, 466, 614
Fsp4HI GCNGC 9 cut(s) 58, 422, 425, 1026, 1029, 2345, 2412, 2415, 2418
FspBI CTAG 1 cut(s) 578
GlaI GCGC 2 cut(s) 24, 413
GluI GCNGC 9 cut(s) 58, 422, 425, 1026, 1029, 2345, 2412, 2415, 2418
GsaI CCCAGC 3 cut(s) 95, 560, 1833
GsuI CTGGAG 2 cut(s) 1617, 1752
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 6 cut(s) 253, 427, 584, 661, 1936, 2321
HapII CCGG 2 cut(s) 250, 466
HgaI GACGC 3 cut(s) 317, 824, 1493
HhaI GCGC 2 cut(s) 25, 414
Hin1I GRCGYC 2 cut(s) 309, 835
Hin6I GCGC 2 cut(s) 23, 412
HinP1I GCGC 2 cut(s) 23, 412
HincII GTYRAC 3 cut(s) 235, 2092, 2200
HindII GTYRAC 3 cut(s) 235, 2092, 2200
HindIII AAGCTT 1 cut(s) 324
HpaII CCGG 2 cut(s) 250, 466
HphI GGTGA 6 cut(s) 19, 584, 817, 1131, 1589, 1732
Hpy188III TCNNGA 6 cut(s) 266, 275, 443, 1369, 1976, 2393
Hpy99I CGWCG 2 cut(s) 203, 305
HpyAV CCTTC 9 cut(s) 295, 463, 947, 1266, 1366, 1973, 2134, 2278, 2366
HpyCH4III ACNGT 6 cut(s) 373, 623, 818, 1681, 1726, 1776
HpyCH4IV ACGT 2 cut(s) 237, 300
HpyF10VI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 1835, 2382, 2417
HpyF3I CTNAG 7 cut(s) 118, 225, 417, 663, 1017, 1308, 1623
HpySE526I ACGT 2 cut(s) 237, 300
Hsp92I GRCGYC 2 cut(s) 309, 835
HspAI GCGC 2 cut(s) 23, 412
KflI GGGWCCC 1 cut(s) 2298
KpnI GGTACC 1 cut(s) 76
Ksp22I TGATCA 4 cut(s) 873, 897, 921, 1759
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 4 cut(s) 15, 248, 1275, 1503
Lsp1109I GCAGC 7 cut(s) 69, 408, 1012, 1040, 2331, 2398, 2401
LweI GCATC 6 cut(s) 703, 1183, 1496, 1757, 2140, 2394
MaeI CTAG 1 cut(s) 578
MaeII ACGT 2 cut(s) 237, 300
MbiI CCGCTC 1 cut(s) 1988
MflI RGATCY 2 cut(s) 1472, 1982
MhlI GDGCHC 6 cut(s) 231, 245, 294, 466, 614, 1817
MlsI TGGCCA 1 cut(s) 2321
MluNI TGGCCA 1 cut(s) 2321
MlyI GAGTC 6 cut(s) 181, 1129, 1238, 1463, 1621, 1861
MmeI TCCRAC 6 cut(s) 222, 721, 1089, 1303, 1545, 2237
Mox20I TGGCCA 1 cut(s) 2321
Mph1103I ATGCAT 2 cut(s) 718, 1794
MroXI GAANNNNTTC 1 cut(s) 2376
MscI TGGCCA 1 cut(s) 2321
MslI CAYNNNNRTG 1 cut(s) 1036
Msp20I TGGCCA 1 cut(s) 2321
MspI CCGG 2 cut(s) 250, 466
MspR9I CCNGG 3 cut(s) 466, 501, 1009
Mva1269I GAATGC 3 cut(s) 716, 1792, 2376
MvaI CCWGG 2 cut(s) 501, 1009
MvnI CGCG 1 cut(s) 1977
MwoI GCNNNNNNNGC 6 cut(s) 162, 289, 418, 1835, 2382, 2417
NciI CCSGG 1 cut(s) 466
NcoI CCATGG 2 cut(s) 1503, 1905
NlaIV GGNNCC 9 cut(s) 17, 74, 89, 554, 583, 939, 1984, 2299, 2300
NmuCI GTSAC 6 cut(s) 836, 956, 1303, 1720, 1726, 2116
NruI TCGCGA 1 cut(s) 1977
NsiI ATGCAT 2 cut(s) 718, 1794
NspI RCATGY 3 cut(s) 844, 999, 1553
NspV TTCGAA 1 cut(s) 1270
PaeI GCATGC 1 cut(s) 999
PasI CCCWGGG 1 cut(s) 500
PciI ACATGT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 988
PctI GAATGC 3 cut(s) 716, 1792, 2376
PdmI GAANNNNTTC 1 cut(s) 2376
PfeI GAWTC 7 cut(s) 38, 520, 655, 1102, 1439, 1685, 1901
PfoI TCCNGGA 1 cut(s) 1007
PkrI GCNGC 9 cut(s) 59, 423, 426, 1027, 1030, 2346, 2413, 2416, 2419
PleI GAGTC 6 cut(s) 181, 1128, 1238, 1462, 1621, 1860
PpsI GAGTC 6 cut(s) 181, 1128, 1238, 1462, 1621, 1860
PpuMI RGGWCCY 1 cut(s) 2298
PscI ACATGT 1 cut(s) 840
PshAI GACNNNNGTC 2 cut(s) 191, 1119
PshBI ATTAAT 1 cut(s) 1173
PsiI TTATAA 1 cut(s) 752
Psp124BI GAGCTC 2 cut(s) 231, 614
Psp5II RGGWCCY 1 cut(s) 2298
Psp6I CCWGG 2 cut(s) 499, 1007
PspFI CCCAGC 3 cut(s) 91, 556, 1829
PspGI CCWGG 2 cut(s) 499, 1007
PspN4I GGNNCC 9 cut(s) 17, 74, 89, 554, 583, 939, 1984, 2299, 2300
PspPI GGNCC 2 cut(s) 582, 2298
PspPPI RGGWCCY 1 cut(s) 2298
PstI CTGCAG 1 cut(s) 1030
PstNI CAGNNNCTG 1 cut(s) 1751
PsuI RGATCY 2 cut(s) 1472, 1982
RruI TCGCGA 1 cut(s) 1977
RsaI GTAC 8 cut(s) 74, 182, 845, 981, 1042, 1450, 2127, 2221
RsaNI GTAC 8 cut(s) 73, 181, 844, 980, 1041, 1449, 2126, 2220
RseI CAYNNNNRTG 1 cut(s) 1036
SacI GAGCTC 2 cut(s) 231, 614
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 9 cut(s) 58, 422, 425, 1026, 1029, 2345, 2412, 2415, 2418
Sau96I GGNCC 2 cut(s) 582, 2298
SchI GAGTC 6 cut(s) 181, 1129, 1238, 1463, 1621, 1861
ScrFI CCNGG 3 cut(s) 466, 501, 1009
SduI GDGCHC 6 cut(s) 231, 245, 294, 466, 614, 1817
SfaNI GCATC 6 cut(s) 703, 1183, 1496, 1757, 2140, 2394
SfcI CTRYAG 2 cut(s) 1026, 2055
SfuI TTCGAA 1 cut(s) 1270
SinI GGWCC 1 cut(s) 2298
SmiMI CAYNNNNRTG 1 cut(s) 1036
SmlI CTYRAG 7 cut(s) 160, 401, 1117, 1608, 1629, 1816, 2177
SmoI CTYRAG 7 cut(s) 160, 401, 1117, 1608, 1629, 1816, 2177
SphI GCATGC 1 cut(s) 999
SsiI CCGC 9 cut(s) 13, 294, 424, 635, 1689, 1928, 1967, 1986, 2417
SspMI CTAG 1 cut(s) 578
SstI GAGCTC 2 cut(s) 231, 614
StyD4I CCNGG 3 cut(s) 464, 499, 1007
StyI CCWWGG 5 cut(s) 76, 151, 1053, 1503, 1905
TaaI ACNGT 6 cut(s) 373, 623, 818, 1681, 1726, 1776
TaiI ACGT 2 cut(s) 240, 303
TaqI TCGA 5 cut(s) 288, 460, 1270, 2134, 2313
TatI WGTACW 3 cut(s) 843, 1040, 2219
TauI GCSGC 2 cut(s) 427, 2420
TfiI GAWTC 7 cut(s) 38, 520, 655, 1102, 1439, 1685, 1901
TscAI CASTG 7 cut(s) 229, 261, 516, 1384, 1546, 1779, 2091
TseFI GTSAC 6 cut(s) 836, 956, 1303, 1720, 1726, 2116
TseI GCWGC 7 cut(s) 57, 421, 1025, 1028, 2344, 2411, 2414
Tsp45I GTSAC 6 cut(s) 836, 956, 1303, 1720, 1726, 2116
TspDTI ATGAA 8 cut(s) 990, 1080, 1413, 1539, 1800, 1970, 2171, 2259
TspGWI ACGGA 2 cut(s) 1688, 2228
TspRI CASTG 7 cut(s) 229, 261, 516, 1384, 1546, 1779, 2091
VpaK11BI GGWCC 1 cut(s) 2298
VspI ATTAAT 1 cut(s) 1173
XceI RCATGY 3 cut(s) 844, 999, 1553
XmiI GTMKAC 3 cut(s) 306, 368, 1428
XmnI GAANNNNTTC 1 cut(s) 2376
XspI CTAG 1 cut(s) 578
Zsp2I ATGCAT 2 cut(s) 718, 1794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.