Rroxscaffold_4G00284500

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000527
Physical Location & Seq
Forward (+)
6089958 .. 6102022
12065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284500.1

Sequence Viewer

Length: 768 bp
ATGATATACTTGAAAAGCCTGCAAATCCAAGGGGGAAACCAAAACCTGGCAACTTACCAAAGAAAGAAAAGGTTCGAACTGACAGGTGTAAAGCATAACGGAACTGGAGTTTTTACTGATGTTGAAGTGTCCAAGACTAATGTTGTTCAACATATAGGTAACCCTGCTGGGACTGTCAACCAACAAGAAAAAAGTCCAAATCTGCCTTTTGTGAAGAATTCTCCACTATGGGAACATCTTGAATCCATGGAAGTATTCAAAAACTATCCACAGAAGCCTCATTTTCATTTTGCGGTGAACTTGAATCCTGTATTTCGTGAAGGAGCAGCCATCGGCAGTATGTTAGCCTTTGCTTCTTTGGTTGAGAAGATTTCCAATTTACGAGTTGATGATCCTAGAGAATTAATCGAAAGCTATTTGAATGGACTAGTTGAAATGGAATTGATGGGATTTGATGTCAAAGCACTAAGGCATCGTATGACTGAATTGCTAGACATTAAAATAAAGTTGGGACAGCTTCAGAAACAATCAAAAGAAGTTAAACTTAGGATCACAGGGAGTACTCATGACACAACCACCTATGATGAAACAATCACTGGGATTGACAAGAAGATTAAGGACTTACAAGAAAAACGGATGATGGCAGTGTCAATGAAAGAGGTCAAAGTTTCTGAAATTAGCAGGTTGCAAGCAGAAGCGACAGCTATCAATGAAGACATCCAGAGTATCAGGTGTGATTTTGGAAACCTAGTGGCTGCTGAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

255

Amino Acids

28.9

Weight (kDa)

7.82

Isoelectric Point (pI)

33.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF724 PF05266 68 - 253 2e-55 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 672
AccB7I CCANNNNNTGG 1 cut(s) 46
AciI CCGC 1 cut(s) 293
AclWI GGATC 2 cut(s) 386, 557
AcsI RAATTY 1 cut(s) 217
AcuI CTGAAG 1 cut(s) 503
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 8 cut(s) 13, 125, 149, 242, 259, 304, 421, 434
AhlI ACTAGT 1 cut(s) 427
AjnI CCWGG 1 cut(s) 45
AluBI AGCT 3 cut(s) 414, 517, 704
AluI AGCT 3 cut(s) 414, 517, 704
AlwI GGATC 2 cut(s) 386, 557
ApeKI GCWGC 2 cut(s) 326, 755
ApoI RAATTY 1 cut(s) 217
AseI ATTAAT 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 307
AsuII TTCGAA 1 cut(s) 75
BbsI GAAGAC 1 cut(s) 720
BbvI GCAGC 2 cut(s) 338, 742
BccI CCATC 3 cut(s) 338, 439, 634
BciT130I CCWGG 1 cut(s) 47
BcuI ACTAGT 1 cut(s) 427
BfaI CTAG 4 cut(s) 396, 428, 491, 749
BfuAI ACCTGC 1 cut(s) 672
BisI GCNGC 2 cut(s) 327, 756
BlsI GCNGC 2 cut(s) 328, 757
BmcAI AGTACT 1 cut(s) 562
Bme1390I CCNGG 1 cut(s) 47
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 1 cut(s) 481
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 28, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 2 cut(s) 109, 601
BseBI CCWGG 1 cut(s) 47
BseDI CCNNGG 2 cut(s) 28, 246
BseGI GGATG 2 cut(s) 642, 717
BseLI CCNNNNNNNGG 1 cut(s) 46
BseNI ACTGG 2 cut(s) 109, 601
BseXI GCAGC 2 cut(s) 338, 742
BseYI CCCAGC 1 cut(s) 167
BslFI GGGAC 2 cut(s) 184, 525
BslI CCNNNNNNNGG 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 184, 525
Bsp119I TTCGAA 1 cut(s) 75
Bsp143I GATC 2 cut(s) 391, 549
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 1 cut(s) 293
BspHI TCATGA 1 cut(s) 565
BspMI ACCTGC 1 cut(s) 672
BspPI GGATC 2 cut(s) 386, 557
BspT104I TTCGAA 1 cut(s) 75
BsrI ACTGG 2 cut(s) 109, 601
BssECI CCNNGG 2 cut(s) 28, 246
BssMI GATC 2 cut(s) 391, 549
BssT1I CCWWGG 2 cut(s) 28, 246
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 175
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 2 cut(s) 20, 690
BstDEI CTNAG 2 cut(s) 467, 545
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 2 cut(s) 642, 717
BstKTI GATC 2 cut(s) 394, 552
BstMBI GATC 2 cut(s) 391, 549
BstNI CCWGG 1 cut(s) 47
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 45
BstV1I GCAGC 2 cut(s) 338, 742
BstV2I GAAGAC 1 cut(s) 720
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 2 cut(s) 642, 717
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 594, 651
BveI ACCTGC 1 cut(s) 672
Cac8I GCNNGC 2 cut(s) 20, 690
CciI TCATGA 1 cut(s) 565
Csp6I GTAC 1 cut(s) 561
CviAII CATG 2 cut(s) 247, 566
CviJI RGCY 8 cut(s) 18, 277, 329, 347, 414, 517, 704, 755
CviKI_1 RGCY 8 cut(s) 18, 277, 329, 347, 414, 517, 704, 755
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 2 cut(s) 467, 545
DpnI GATC 2 cut(s) 393, 551
DpnII GATC 2 cut(s) 391, 549
Eco130I CCWWGG 2 cut(s) 28, 246
Eco57I CTGAAG 1 cut(s) 503
Eco91I GGTNACC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 28, 246
ErhI CCWWGG 2 cut(s) 28, 246
FaeI CATG 2 cut(s) 250, 569
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FaqI GGGAC 2 cut(s) 184, 525
FatI CATG 2 cut(s) 246, 565
Fnu4HI GCNGC 2 cut(s) 327, 756
FokI GGATG 2 cut(s) 649, 704
Fsp4HI GCNGC 2 cut(s) 327, 756
FspBI CTAG 4 cut(s) 396, 428, 491, 749
GluI GCNGC 2 cut(s) 327, 756
GsaI CCCAGC 1 cut(s) 171
GsuI CTGGAG 1 cut(s) 126
Hin1II CATG 2 cut(s) 250, 569
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 2 cut(s) 242, 304
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 2 cut(s) 178, 298
Hpy188I TCNGA 2 cut(s) 522, 673
Hpy188III TCNNGA 4 cut(s) 239, 317, 566, 721
Hpy8I GTNNAC 2 cut(s) 178, 298
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 2 cut(s) 22, 688
HpyF3I CTNAG 2 cut(s) 467, 545
Hsp92II CATG 2 cut(s) 250, 569
Kzo9I GATC 2 cut(s) 391, 549
LmnI GCTCC 1 cut(s) 323
Lsp1109I GCAGC 2 cut(s) 338, 742
LweI GCATC 1 cut(s) 481
MaeI CTAG 4 cut(s) 396, 428, 491, 749
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 393, 551
MboI GATC 2 cut(s) 391, 549
MboII GAAGA 4 cut(s) 226, 379, 622, 725
MluCI AATT 6 cut(s) 217, 376, 401, 440, 485, 675
MnlI CCTC 2 cut(s) 288, 652
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 4 cut(s) 404, 498, 540, 615
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 246
NdeII GATC 2 cut(s) 391, 549
NlaIII CATG 2 cut(s) 250, 569
NspV TTCGAA 1 cut(s) 75
PagI TCATGA 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 2 cut(s) 242, 304
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 2 cut(s) 328, 757
PshBI ATTAAT 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 45
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 167
PspGI CCWGG 1 cut(s) 45
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
SaqAI TTAA 4 cut(s) 404, 498, 540, 615
SatI GCNGC 2 cut(s) 327, 756
Sau3AI GATC 2 cut(s) 391, 549
ScaI AGTACT 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 47
SfaNI GCATC 1 cut(s) 481
SfuI TTCGAA 1 cut(s) 75
SpeI ACTAGT 1 cut(s) 427
Sse9I AATT 6 cut(s) 217, 376, 401, 440, 485, 675
SsiI CCGC 1 cut(s) 293
SspMI CTAG 4 cut(s) 396, 428, 491, 749
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 2 cut(s) 28, 246
TaaI ACNGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 75, 408
TasI AATT 6 cut(s) 217, 376, 401, 440, 485, 675
TatI WGTACW 1 cut(s) 560
TfiI GAWTC 2 cut(s) 242, 304
Tru1I TTAA 4 cut(s) 404, 498, 540, 615
Tru9I TTAA 4 cut(s) 404, 498, 540, 615
TscAI CASTG 2 cut(s) 601, 651
TseI GCWGC 2 cut(s) 326, 755
TspDTI ATGAA 4 cut(s) 275, 600, 668, 726
TspGWI ACGGA 2 cut(s) 114, 649
TspRI CASTG 2 cut(s) 601, 651
Van91I CCANNNNNTGG 1 cut(s) 46
VspI ATTAAT 1 cut(s) 404
XapI RAATTY 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 4 cut(s) 396, 428, 491, 749
ZrmI AGTACT 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.