MD07G1240300.v1.1

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000527
Physical Location & Seq
Reverse (-)
31171262 .. 31176269
5008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1240300.v1.1.491

Sequence Viewer

Length: 2262 bp
ATGGTGGTCAAGAGAAGTCAATCATCAACCCATTTGAGCAAGGATTCAAAGGTGGAGGTTTGCAGCAATGAAGATGGCTACAAGGGTGTTTGGTTCCCCGCCAAAATCATCGACCCGGACCCCCCGATTCCGGCCACGAGGAAGAAAAGGAAGAGCCTTTCCGGCTGCAGCAGCAGAAGCTCATCCTCCTCCACCAAGGCTCTGGTCCAGTACGAGACTCTGCTCTCCGACGATGACCCGGACAAGCCCCTGACGGAGCTCGTCGAGGTGGGTTTCATCCGGCCGGTGCCTCCGTCCGACATTGACGACAGGCCTTTCGAGCCCGGTGACAAAGTCGACGCCTTTCACCTCGACGCGTGGTGGCCCGGGGTTGTGATCAAGCTCGAGGAGGATGAGTACACGGTGGGGTTTCTGGACCCGCCTGATCTGCTTGTGCTGCGGCGGTCCGATTTGCGGTCGCACTGGGATTTCACAGACGGCATTTGGGTCGGAGCTAAGAAGGAGACGATGACAGGACCAAATTTCATTCCTGGGACAGCAGTTGAAGTGAATCCCAGCAAAGTAATACCTTTTTATGCTTGGTTTCCAGCAATTTATCTTGGGCAGCTGGGGATCGACTCTTTCTTGCTTCAATATAAGAGATCAAAGAACTGGGATGTTAAAACTGTAGTGTGTCGCCATCAGATTCGGCCTCAGCCTCCACTGTCGGATGAAAGAGATTTTAGCTTGTTGGATAAAGTGGATGCATTCCATGACATGGGTTGGTGGGTCGGAGAGATTACGAAAGTTCTTGAAGGAAACAAGTACGTGGTCACTTTAAGCTGCACAAGGCAGGAGATGGAATTCAGCATTTCAGAATTGAGGACTCACTTAGTTTGGCACGATGAAAGATGGAGTAATGAATTTGCGGAATCTCACTTTAGAAGGGATTCTCAAGTACCAACAAAGCATTCATGTCAGAGTTCCAGAAGTGATTACGAATTACAATCATGCAAGAGTTTTGGAAACTCTGAGGTAGCTACACCACTTCGAAAGAAAACACCTTGTTCTAGGATTTCATTGAAAAATCAATCGAAGCACTTGAGTCCTTGCATGGATAAATTACCTTATGTAATGACGAAAAAGAAATCGAAAAAGTCTCAGCAACCTAAAGATGATGAAACAATTGCATGTTTTTCCAAGAAGTTAAAGGGTGGATACTCAGAAGACTCCTTATCGTTTGAAAAATCAAATGCAAGGAGAACGCCCGCTAAAACTCAAAACAAAGAATCCCCAGTAACTGATGTCGTGAAGATTGAGAAACCGAAGATTGGACGACTGGATGATCAAGCATTAACTCATTCTAAAAGAAAAGGTAACCCTTCTGGGACTGCCAACCAACAAAATGAGGATAATGGAAGACAGGTTGAAGCTGGTGTCACAGGTAACCCTGTGGGGACTCCCAACAAACAAAACGAGGTTAACGGAAGACAAGTTGAAACTGTTGTCACAGATTGCTCGACAAGAGAACTTAAGGTGGCTGCTGTAAAACCTATAGAGGGCTCAAGTAGCCCTGCCGAGACTGCCAACCAACAAAATGATGTTACAGGAACACAAGTTGAAACTGGTGTTACTGTTAACATTGAGCAACAAGACAGTCCAGATTTGCCATTTGTGAAATCTTATCCGGAAATTTGGGAGAAAGTTGAATCTATGGATGCATTTAAAAGATATCCGCAAAAACCACATTTTTATCCTTTAGTTAAATGTGGAGAGCTAAGTCGTGAATCATTAGCGGTTGCAAAGATGTTAACCTTTGCCTCTTTGGTAGAGAAGACCTCCAAGTTGAACGTTGAGGATCCTGACGACTTCTTTGATAGCATCTTCGAAGAACTAGGTGATTTGGAAACGTTTGGATTTGATGTCAAGGCAGTACACGATCGTCTGAACTCTTTGCTAGAAATGAAAGTTCAGTTGGGGCACCTTCAGGATAAGTCAAAAGAAGTTCAAATCAAGATCGTGGAGCAAACTCAAGACAGAAAGAAACATAATGAAATAATCAGTGGGGTTGCTAAGCAGATTAAGGACTTGCAGGATCAACTCGTGGTGCTGATGTCAGAGGACGCAGCCAAAGGTTCTGAAATTAGCAGGTTGCAATCCGAAGCAGACGCTATCATTGAAAGCATTCAGAGCATCCGCCGTATGAGACACTGGCTGAAGCATGGTGATCCTCGTGAAAACGTTCATTTGATTACCGCTTTGTCTGCGGAAACTGAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

754

Amino Acids

85.03

Weight (kDa)

6.06

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 98 3.7e-14 Agenet domain
DUF724 PF05266 549 - 726 4.5e-47 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2118
AccB1I GGYRCC 2 cut(s) 286, 1959
AccB7I CCANNNNNTGG 1 cut(s) 757
AccI GTMKAC 1 cut(s) 336
AccII CGCG 1 cut(s) 356
AccIII TCCGGA 1 cut(s) 1666
AclI AACGTT 3 cut(s) 1830, 1889, 2220
AclWI GGATC 5 cut(s) 620, 1832, 1845, 2082, 2201
AcoI YGGCCR 2 cut(s) 132, 281
AcsI RAATTY 4 cut(s) 520, 842, 902, 1671
AcuI CTGAAG 2 cut(s) 1949, 2216
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 5 cut(s) 212, 398, 806, 939, 1914
AfiI CCNNNNNNNGG 5 cut(s) 130, 453, 757, 1310, 1538
AflII CTTAAG 1 cut(s) 1511
AflIII ACRYGT 1 cut(s) 354
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 4 cut(s) 934, 966, 1937, 1969
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 5 cut(s) 209, 497, 1143, 1553, 2179
AlwI GGATC 5 cut(s) 620, 1832, 1845, 2082, 2201
AlwNI CAGNNNCTG 1 cut(s) 1280
Ama87I CYCGRG 2 cut(s) 365, 383
Aor13HI TCCGGA 1 cut(s) 1666
AoxI GGCC 5 cut(s) 132, 281, 311, 362, 689
ApeKI GCWGC 9 cut(s) 63, 165, 168, 171, 436, 604, 822, 1520, 2105
ApoI RAATTY 4 cut(s) 520, 842, 902, 1671
ArsI GACNNNNNNTTYG 4 cut(s) 299, 331, 1835, 1867
Asp700I GAANNNNTTC 3 cut(s) 928, 2163, 2220
AspS9I GGNCC 6 cut(s) 118, 205, 363, 415, 444, 515
AsuC2I CCSGG 5 cut(s) 116, 239, 324, 366, 367
AsuHPI GGTGA 4 cut(s) 338, 338, 1889, 2216
AsuII TTCGAA 2 cut(s) 1030, 1866
AvaI CYCGRG 2 cut(s) 365, 383
AvaII GGWCC 5 cut(s) 118, 205, 415, 444, 515
BaeGI GKGCMC 1 cut(s) 1962
BamHI GGATCC 1 cut(s) 1837
BanI GGYRCC 2 cut(s) 286, 1959
BanII GRGCYC 3 cut(s) 261, 324, 1544
BauI CACGAG 3 cut(s) 136, 2081, 2211
BbsI GAAGAC 4 cut(s) 1212, 1405, 1474, 1820
Bbv12I GWGCWC 1 cut(s) 261
BbvCI CCTCAGC 1 cut(s) 693
BbvI GCAGC 9 cut(s) 75, 152, 180, 183, 423, 616, 809, 1507, 2117
BccI CCATC 4 cut(s) 68, 687, 832, 885
BceAI ACGGC 2 cut(s) 493, 2163
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 1 cut(s) 1190
BclI TGATCA 2 cut(s) 375, 1324
BcnI CCSGG 5 cut(s) 116, 239, 324, 366, 367
BcoDI GTCTC 5 cut(s) 209, 497, 1143, 1553, 2179
BfaI CTAG 3 cut(s) 1050, 1874, 1937
BfmI CTRYAG 3 cut(s) 166, 666, 1533
BfrI CTTAAG 1 cut(s) 1511
BfuAI ACCTGC 1 cut(s) 2118
BfuI GTATCC 1 cut(s) 1190
BglI GCCNNNNNGGC 1 cut(s) 162
BlpI GCTNAGC 1 cut(s) 2052
Bme1390I CCNGG 6 cut(s) 116, 239, 324, 366, 367, 531
Bme18I GGWCC 5 cut(s) 118, 205, 415, 444, 515
BmeT110I CYCGRG 2 cut(s) 365, 383
BmgT120I GGNCC 6 cut(s) 118, 205, 363, 415, 444, 515
BmiI GGNNCC 6 cut(s) 95, 120, 288, 417, 1839, 1961
BmrFI CCNGG 6 cut(s) 116, 239, 324, 366, 367, 531
BmrI ACTGGG 3 cut(s) 472, 661, 1268
BmsI GCATC 4 cut(s) 733, 1687, 1869, 2181
BmuI ACTGGG 3 cut(s) 472, 661, 1268
BpiI GAAGAC 4 cut(s) 1212, 1405, 1474, 1820
BplI GAGNNNNNCTC 2 cut(s) 1802, 1834
Bpu10I CCTNAGC 1 cut(s) 693
Bpu1102I GCTNAGC 1 cut(s) 2052
Bpu14I TTCGAA 2 cut(s) 1030, 1866
BpuEI CTTGAG 4 cut(s) 918, 1102, 1528, 1995
BpuMI CCSGG 5 cut(s) 116, 239, 324, 366, 367
BsaAI YACGTR 1 cut(s) 808
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 4 cut(s) 195, 365, 366, 530
BsaWI WCCGGW 1 cut(s) 1666
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 5 cut(s) 130, 453, 757, 1310, 1538
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 7 cut(s) 208, 467, 656, 1274, 1323, 1609, 2195
Bse3DI GCAATG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 1666
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 4 cut(s) 195, 365, 366, 530
BseGI GGATG 9 cut(s) 182, 276, 397, 661, 715, 748, 1327, 1702, 2172
BseLI CCNNNNNNNGG 5 cut(s) 130, 453, 757, 1310, 1538
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 4 cut(s) 707, 1002, 1154, 1215
BseNI ACTGG 7 cut(s) 208, 467, 656, 1274, 1323, 1609, 2195
BseRI GAGGAG 2 cut(s) 178, 401
BseSI GKGCMC 1 cut(s) 1962
BseX3I CGGCCG 1 cut(s) 281
BseXI GCAGC 9 cut(s) 75, 152, 180, 183, 423, 616, 809, 1507, 2117
BseYI CCCAGC 2 cut(s) 554, 607
BsgI GTGCAG 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 356
Bsh1285I CGRYCG 3 cut(s) 284, 458, 1921
BshFI GGCC 5 cut(s) 134, 283, 313, 364, 691
BshNI GGYRCC 2 cut(s) 286, 1959
BsiEI CGRYCG 3 cut(s) 284, 458, 1921
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 2 cut(s) 365, 383
BsiSI CCGG 9 cut(s) 116, 131, 162, 239, 280, 284, 324, 366, 1667
BslFI GGGAC 3 cut(s) 547, 1381, 1450
BslI CCNNNNNNNGG 5 cut(s) 130, 453, 757, 1310, 1538
BsmAI GTCTC 5 cut(s) 209, 497, 1143, 1553, 2179
BsmBI CGTCTC 1 cut(s) 497
BsmFI GGGAC 3 cut(s) 547, 1381, 1450
BsmI GAATGC 3 cut(s) 746, 949, 2163
BsnI GGCC 5 cut(s) 134, 283, 313, 364, 691
BsoBI CYCGRG 2 cut(s) 365, 383
Bsp119I TTCGAA 2 cut(s) 1030, 1866
Bsp1286I GDGCHC 4 cut(s) 261, 324, 1544, 1962
Bsp13I TCCGGA 1 cut(s) 1666
Bsp1720I GCTNAGC 1 cut(s) 2052
BspANI GGCC 5 cut(s) 134, 283, 313, 364, 691
BspCNI CTCAG 4 cut(s) 706, 1003, 1153, 1214
BspEI TCCGGA 1 cut(s) 1666
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 6 cut(s) 95, 120, 288, 417, 1839, 1961
BspMAI CTGCAG 1 cut(s) 170
BspMI ACCTGC 1 cut(s) 2118
BspPI GGATC 5 cut(s) 620, 1832, 1845, 2082, 2201
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 2 cut(s) 1030, 1866
BspT107I GGYRCC 2 cut(s) 286, 1959
BspTI CTTAAG 1 cut(s) 1511
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 283
BsrI ACTGG 7 cut(s) 208, 467, 656, 1274, 1323, 1609, 2195
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 4 cut(s) 195, 365, 366, 530
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 3 cut(s) 136, 2081, 2211
BssT1I CCWWGG 1 cut(s) 195
Bst2BI CACGAG 3 cut(s) 136, 2081, 2211
Bst2UI CCWGG 1 cut(s) 531
Bst4CI ACNGT 6 cut(s) 403, 667, 705, 1483, 1615, 1637
Bst6I CTCTTC 1 cut(s) 146
BstACI GRCGYC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 1511
BstBAI YACGTR 1 cut(s) 808
BstBI TTCGAA 2 cut(s) 1030, 1866
BstC8I GCNNGC 1 cut(s) 1248
BstDEI CTNAG 8 cut(s) 495, 693, 871, 1011, 1140, 1201, 1757, 2052
BstEII GGTNACC 2 cut(s) 1355, 1424
BstF5I GGATG 9 cut(s) 182, 276, 397, 661, 715, 748, 1327, 1702, 2172
BstFNI CGCG 1 cut(s) 356
BstMAI GTCTC 5 cut(s) 209, 497, 1143, 1553, 2179
BstMCI CGRYCG 3 cut(s) 284, 458, 1921
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 1 cut(s) 1173
BstPI GGTNACC 2 cut(s) 1355, 1424
BstSCI CCNGG 6 cut(s) 114, 237, 322, 364, 365, 529
BstSFI CTRYAG 3 cut(s) 166, 666, 1533
BstSLI GKGCMC 1 cut(s) 1962
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 9 cut(s) 75, 152, 180, 183, 423, 616, 809, 1507, 2117
BstV2I GAAGAC 4 cut(s) 1212, 1405, 1474, 1820
BstX2I RGATCY 1 cut(s) 1837
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 1837
BstZI CGGCCG 1 cut(s) 281
BsuI GTATCC 1 cut(s) 1190
BsuRI GGCC 5 cut(s) 134, 283, 313, 364, 691
BtsCI GGATG 9 cut(s) 182, 276, 397, 661, 715, 748, 1327, 1702, 2172
BtsIMutI CAGTG 4 cut(s) 460, 701, 2047, 2188
BveI ACCTGC 1 cut(s) 2118
Cac8I GCNNGC 1 cut(s) 1248
CaiI CAGNNNCTG 1 cut(s) 1280
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 6 cut(s) 118, 205, 363, 415, 444, 515
Cfr9I CCCGGG 1 cut(s) 365
CpoI CGGWCCG 1 cut(s) 444
CseI GACGC 4 cut(s) 347, 362, 2111, 2156
Csp6I GTAC 5 cut(s) 211, 397, 805, 938, 1913
CspI CGGWCCG 1 cut(s) 444
CviAII CATG 7 cut(s) 752, 757, 954, 990, 1093, 1170, 2201
CviQI GTAC 5 cut(s) 211, 397, 805, 938, 1913
DdeI CTNAG 8 cut(s) 495, 693, 871, 1011, 1140, 1201, 1757, 2052
DraI TTTAAA 1 cut(s) 1705
EaeI YGGCCR 2 cut(s) 132, 281
EagI CGGCCG 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 2165
Ecl136II GAGCTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 281
Eco130I CCWWGG 1 cut(s) 195
Eco147I AGGCCT 1 cut(s) 313
Eco24I GRGCYC 3 cut(s) 261, 324, 1544
Eco32I GATATC 1 cut(s) 1712
Eco47I GGWCC 5 cut(s) 118, 205, 415, 444, 515
Eco52I CGGCCG 1 cut(s) 281
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 1949, 2216
Eco88I CYCGRG 2 cut(s) 365, 383
Eco91I GGTNACC 2 cut(s) 1355, 1424
EcoICRI GAGCTC 1 cut(s) 259
EcoO65I GGTNACC 2 cut(s) 1355, 1424
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 1 cut(s) 529
EcoRV GATATC 1 cut(s) 1712
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 2 cut(s) 748, 1702
EcoT38I GRGCYC 3 cut(s) 261, 324, 1544
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 1 cut(s) 497
FaeI CATG 7 cut(s) 755, 760, 957, 993, 1096, 1173, 2204
FaqI GGGAC 3 cut(s) 547, 1381, 1450
FatI CATG 7 cut(s) 751, 756, 953, 989, 1092, 1169, 2200
FauI CCCGC 3 cut(s) 106, 426, 1255
FbaI TGATCA 2 cut(s) 375, 1324
FblI GTMKAC 1 cut(s) 336
FokI GGATG 9 cut(s) 169, 263, 404, 668, 722, 755, 1334, 1709, 2159
FriOI GRGCYC 3 cut(s) 261, 324, 1544
FspBI CTAG 3 cut(s) 1050, 1874, 1937
GsaI CCCAGC 2 cut(s) 558, 611
HaeIII GGCC 5 cut(s) 134, 283, 313, 364, 691
HapII CCGG 9 cut(s) 116, 131, 162, 239, 280, 284, 324, 366, 1667
HgaI GACGC 4 cut(s) 347, 362, 2111, 2156
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 7 cut(s) 755, 760, 957, 993, 1096, 1173, 2204
HincII GTYRAC 4 cut(s) 337, 1462, 1618, 1791
HindII GTYRAC 4 cut(s) 337, 1462, 1618, 1791
HpaI GTTAAC 3 cut(s) 1462, 1618, 1791
HpaII CCGG 9 cut(s) 116, 131, 162, 239, 280, 284, 324, 366, 1667
HphI GGTGA 4 cut(s) 338, 338, 1889, 2216
Hpy166II GTNNAC 6 cut(s) 337, 399, 1462, 1618, 1791, 1915
Hpy8I GTNNAC 6 cut(s) 337, 399, 1462, 1618, 1791, 1915
Hpy99I CGWCG 4 cut(s) 233, 266, 341, 356
HpyAV CCTTC 5 cut(s) 493, 788, 918, 1371, 1973
HpyCH4III ACNGT 6 cut(s) 403, 667, 705, 1483, 1615, 1637
HpyCH4IV ACGT 4 cut(s) 807, 1830, 1889, 2220
HpyF3I CTNAG 8 cut(s) 495, 693, 871, 1011, 1140, 1201, 1757, 2052
HpySE526I ACGT 4 cut(s) 807, 1830, 1889, 2220
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 7 cut(s) 755, 760, 957, 993, 1096, 1173, 2204
Kpn2I TCCGGA 1 cut(s) 1666
Ksp22I TGATCA 2 cut(s) 375, 1324
KspAI GTTAAC 3 cut(s) 1462, 1618, 1791
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 3 cut(s) 256, 491, 2002
Lsp1109I GCAGC 9 cut(s) 75, 152, 180, 183, 423, 616, 809, 1507, 2117
LweI GCATC 4 cut(s) 733, 1687, 1869, 2181
MaeI CTAG 3 cut(s) 1050, 1874, 1937
MaeII ACGT 4 cut(s) 807, 1830, 1889, 2220
MaeIII GTNAC 9 cut(s) 326, 811, 1276, 1355, 1417, 1424, 1486, 1582, 1609
MfeI CAATTG 1 cut(s) 1164
MflI RGATCY 1 cut(s) 1837
MhlI GDGCHC 4 cut(s) 261, 324, 1544, 1962
MluI ACGCGT 1 cut(s) 354
MlyI GAGTC 6 cut(s) 211, 611, 859, 1093, 1202, 1432
MmeI TCCRAC 6 cut(s) 252, 321, 469, 687, 711, 751
Mph1103I ATGCAT 2 cut(s) 748, 1702
MroI TCCGGA 1 cut(s) 1666
MroXI GAANNNNTTC 3 cut(s) 928, 2163, 2220
MspA1I CMGCKG 1 cut(s) 607
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 9 cut(s) 116, 131, 162, 239, 280, 284, 324, 366, 1667
MspR9I CCNGG 6 cut(s) 116, 239, 324, 366, 367, 531
MunI CAATTG 1 cut(s) 1164
Mva1269I GAATGC 3 cut(s) 746, 949, 2163
MvaI CCWGG 1 cut(s) 531
MvnI CGCG 1 cut(s) 356
NciI CCSGG 5 cut(s) 116, 239, 324, 366, 367
NlaIII CATG 7 cut(s) 755, 760, 957, 993, 1096, 1173, 2204
NlaIV GGNNCC 6 cut(s) 95, 120, 288, 417, 1839, 1961
NmeAIII GCCGAG 1 cut(s) 1582
NmuCI GTSAC 4 cut(s) 326, 811, 1417, 1486
NsiI ATGCAT 2 cut(s) 748, 1702
NspI RCATGY 1 cut(s) 1173
NspV TTCGAA 2 cut(s) 1030, 1866
PaeR7I CTCGAG 1 cut(s) 383
PceI AGGCCT 1 cut(s) 313
PciSI GCTCTTC 1 cut(s) 146
PctI GAATGC 3 cut(s) 746, 949, 2163
PdmI GAANNNNTTC 3 cut(s) 928, 2163, 2220
PfeI GAWTC 9 cut(s) 44, 127, 550, 685, 911, 929, 1268, 1688, 1766
PflFI GACNNNGTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 757
Ple19I CGATCG 1 cut(s) 1921
PleI GAGTC 6 cut(s) 211, 611, 859, 1092, 1202, 1432
PpsI GAGTC 6 cut(s) 211, 611, 859, 1092, 1202, 1432
Ppu21I YACGTR 1 cut(s) 808
Psp124BI GAGCTC 1 cut(s) 261
Psp1406I AACGTT 3 cut(s) 1830, 1889, 2220
Psp6I CCWGG 1 cut(s) 529
PspEI GGTNACC 2 cut(s) 1355, 1424
PspFI CCCAGC 2 cut(s) 554, 607
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 6 cut(s) 95, 120, 288, 417, 1839, 1961
PspPI GGNCC 6 cut(s) 118, 205, 363, 415, 444, 515
PspXI VCTCGAGB 1 cut(s) 383
PstI CTGCAG 1 cut(s) 170
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 1 cut(s) 1837
PsyI GACNNNGTC 1 cut(s) 332
PvuI CGATCG 1 cut(s) 1921
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 5 cut(s) 212, 398, 806, 939, 1914
RsaNI GTAC 5 cut(s) 211, 397, 805, 938, 1913
Rsr2I CGGWCCG 1 cut(s) 444
RsrII CGGWCCG 1 cut(s) 444
SacI GAGCTC 1 cut(s) 261
SalI GTCGAC 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 146
Sau96I GGNCC 6 cut(s) 118, 205, 363, 415, 444, 515
SchI GAGTC 6 cut(s) 211, 611, 859, 1093, 1202, 1432
ScrFI CCNGG 6 cut(s) 116, 239, 324, 366, 367, 531
SduI GDGCHC 4 cut(s) 261, 324, 1544, 1962
SfaNI GCATC 4 cut(s) 733, 1687, 1869, 2181
SfcI CTRYAG 3 cut(s) 166, 666, 1533
Sfr274I CTCGAG 1 cut(s) 383
SfuI TTCGAA 2 cut(s) 1030, 1866
SinI GGWCC 5 cut(s) 118, 205, 415, 444, 515
SlaI CTCGAG 1 cut(s) 383
SmaI CCCGGG 1 cut(s) 367
SmlI CTYRAG 6 cut(s) 383, 933, 1081, 1511, 1543, 2010
SmoI CTYRAG 6 cut(s) 383, 933, 1081, 1511, 1543, 2010
SseBI AGGCCT 1 cut(s) 313
SspMI CTAG 3 cut(s) 1050, 1874, 1937
SstI GAGCTC 1 cut(s) 261
StuI AGGCCT 1 cut(s) 313
StyD4I CCNGG 6 cut(s) 114, 237, 322, 364, 365, 529
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 6 cut(s) 403, 667, 705, 1483, 1615, 1637
TaiI ACGT 4 cut(s) 810, 1833, 1892, 2223
TatI WGTACW 2 cut(s) 396, 1912
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 9 cut(s) 44, 127, 550, 685, 911, 929, 1268, 1688, 1766
TscAI CASTG 4 cut(s) 467, 708, 2047, 2195
TseFI GTSAC 4 cut(s) 326, 811, 1417, 1486
TseI GCWGC 9 cut(s) 63, 165, 168, 171, 436, 604, 822, 1520, 2105
Tsp45I GTSAC 4 cut(s) 326, 811, 1417, 1486
TspGWI ACGGA 3 cut(s) 269, 282, 1479
TspMI CCCGGG 1 cut(s) 365
TspRI CASTG 4 cut(s) 467, 708, 2047, 2195
Tth111I GACNNNGTC 1 cut(s) 332
Van91I CCANNNNNTGG 1 cut(s) 757
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 5 cut(s) 118, 205, 415, 444, 515
XapI RAATTY 4 cut(s) 520, 842, 902, 1671
XceI RCATGY 1 cut(s) 1173
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XhoI CTCGAG 1 cut(s) 383
XmaI CCCGGG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 3 cut(s) 928, 2163, 2220
XspI CTAG 3 cut(s) 1050, 1874, 1937
Zsp2I ATGCAT 2 cut(s) 748, 1702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.