pycom01g18520

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000527
Physical Location & Seq
Reverse (-)
17487557 .. 17493496
5940 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18520.9

Sequence Viewer

Length: 3078 bp
ATGGTGGTCAAGAAAAGTCAATCAGCAACCCATTTGATCAAGGATTCAAAGGTGGAAGTTTGCAGCCTTGAAGATGGCTTCAAGGGCGCATGGTTCCCCGCCAAAATCATCGACTCGAAGCCCCTGATTCCGGCGACGAAGAAGAAAAGGAAGAGCTTTTCTGACGGCAGCAGTAACTCCTTCTCACCAACCAAGGCTGTGGTCCAGTACGAGACTCTGCTCTCCGACGACGACCCGGACAAGCCCCTGACGGAGTTTGTCGAGATGGGTCAAATCCGGCCGGTGCCTCCGGACGACAATGTCGACAGGCCCTTCGAGCCCGGCGACAAAGTCGACGCCTTTCACCTCGACGCGTGGTGGCCCGGGGTTGTAATCAAGCGCGAGGAGGATGAGTACACGGTGGGGTTCATGTACCCGCCTGATCTGCTTGTGGTGCGGCGGTCCGAATTGCGTTCTCACTGGGATTTGGCGGACGGCGTTTGGGTCCGAGCCAAAAAGGAGATGATGATGGTATCAAATTTCAGCCCTGGGACAGCAGTTGAAATGAATCCGGACGAAGAAAGACTATTTTATGCTTGGTTTCCAGCAATTTATCTTGGTCAACTGGGGATCGACTCTTTCTTGCTTCAATATAAGAAATCAAACAACAGTGATGTTAAAATTGTTGTACGTGGCCATCAGATTCGGCCTCAGCCTCCAAATTCGACCGAAAGAGATTTTAGCTTGTTGGATAAAGTGGATGCATTCCATGACATGGGTTGGTGGGTCGGAGATATTACAAAAGTTCTTAAAGGAAACAAGTATGCGGTCACTTTAAGTTGCACGAGGCAGGAGAAGGAATTCAGCCTTTCAGAATTGAGGACTCACATGGATTGGAATGATGGAGGATGGGCTACTATGAGCCATGGAAGATGGATTACTGAAGTTCGGGAATTTCACTTTAGAAGGGGGTCTGAAGCACAATCAAAGCATTCATGTCAGAGTTCCAAACGTGATTATGAATTACAATCATGTGAGAGTTTCGGAAACTCTGACGTAGGTACACCACTTCGAAAGAAAACACCTTGTTCTAGGATCTCAGTGAAAATTCAATCGAAGCCCTTGAGTCCTTGCACGGATAAATTACCTTATGGAAAGACCAAAAAGAAATTGAAAATGTCTCTGCAACCTAAAGATGATGCAACAATTTTAAGTCTTTACAAGAAGTTAAAAGGTGGGCATTCAGATGACTCCTTATCTTTGGAGAAACCATTTGCAAGGAGAACGCTGATTAAAACTCTAAACAAAGAATCCCCAGTTATTAATGTTTTGAAGATTGCGAAACAGAAGGTTAGAGGACTAGATGATCAAGCATTAACTCATTTCAAAAGAAAAGGCAAGAAAAGCAGTGAAATCGAAAAGACCGGTGAAGTAAATGTTGGAGATGAGCATGTAATGGACAACACTGAATCTTCTGGTATCAAGTCAGAGTCTGAAGCAACAGGGGGATCTCATGCTGAAGCATCATGTCTGCTCCGTATGGAGGGGGTTGGACAGAATGAAGATGGAATGAGCAGTGCAGTAGAAGGGAATGGAAAACTGACTGCATTGCTTGTTGAAGGCGAGGAACATAATGGCGGTGGATCTATGGCTGCAGATTGCTCGAATGATATATTCGAATTGTCTATGATCACAAGGTTGGATTTGAAAGGTGAGAAGCACGATGGAACTGGAGTTGTTGCTCAAGTTGAATCGACTGAGACTCAAGTTGCAAAAGATAAAGGTTCTGAAGATGCAATGGTCAAAGAGGAAGCTGTACATTCTGCAATGGATATCGATAAAGGCATTACAAGTATTACAGTGGGCTCAAGTAACCCTGCTGGGATTTCCAAACAACAAAGTGAGGTCAGACAACAGGTTGAAACTGGCGTAACAGGTTCTGAAGAAGCAATGAGAGAGATGGAAACTGTGAATTCGACAATGGATTACTTGACACAAGAAGGTCAGGTGGCTGTGACTGGAGTATTGCCAAAGACATCTGCTCATGATCAACCTTTATCACTGTGCATAAATGAAATGCATTCTGTAAAAACCATAGGTGGTACAAGTAACCCTGCTGGGACTGCCAGCCAACAAAATGAGGTTAATGGAAGACAAGTTGAAATTAGTGTCACAGGTAACCTTGATGGGACTCCGAACCAACAAAACGAGGTTAATGGAAGACAAGTTGAAACTGGTGTCACAGATGGGGCCGATGCGGAGCAAAACAACAATGCAGTGAGACAGGTGGAAACTGTTGATTCTCCAATGGGTTGCTTGACAAAAGAACTTCAGGTGGCTGTGACTGGAATATCCGAAAAGGCATCTGCTCATGATCAACCCTTTTTGTTGTGCACAAGTGAAATGTATTCTGTCAAACCTACAGAGGGCTCAAGTAACCCAGCCGAGACTGATAACCAACAAAATGAGGTTACAGGTACACAAGTTGAAACTGGTGTCACTGTTAACATTGAGCAACAAGACAGTCCAGATTCGCCATTTGTGAAATCTTATCCTGAAATTTGGCAAAAAGTTGAATCTATGGATGCATTTAGAAGATATCCGCAAAAACCACATTTTTATCCTTTAGTTAAGTGTGGAGAGCTTAGTCGTGAATCATTAGCCATTGCAAAGATGCTAACCTTTGCCTCTTTGGTTGAGAAGACCTCCAAGTTGAACATTGAGGTTCCTGACGACTTCTTTGATAGCAGCTTCCAAGAACTAAGTGATTTAGAAACGTTTGGATTTGATGTCAAGGGAGTACGTGACCGTCTGAACTCTTTGCTAGAAATGAAAGTTCAGTTGGGGCAGCTTCAGGACAAGTCAAAGGAAGTTGAAATTCAGATTGCTGAGCAAACTCAAGAAAGAAAGAAACATAATGAAAAAATCAGTGGGGTTGCTAAGCAGATTAAGGACTTGCAGGATCAACTGGTGGTGCTAATGTCAGAGGATGCGGCCAAGGGTACTGAAATTAGCAGGTTGCAATCCGAAGAAAACGCCATCACTGAAAGCATTCTGAGCACCCGTTGTAACTTTGAGAAACTGAGCGAAGCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

1026

Amino Acids

113.56

Weight (kDa)

5.25

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
Acc36I ACCTGC 1 cut(s) 2985
AccB1I GGYRCC 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 2 cut(s) 303, 333
AccII CGCG 2 cut(s) 353, 381
AccIII TCCGGA 2 cut(s) 289, 550
AclI AACGTT 1 cut(s) 2756
AclWI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2949
AcoI YGGCCR 3 cut(s) 278, 673, 2973
AcsI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2538, 2856
AcuI CTGAAG 8 cut(s) 942, 975, 1494, 1518, 1788, 1941, 2294, 2816
AcyI GRCGYC 1 cut(s) 336
AfiI CCNNNNNNNGG 4 cut(s) 130, 754, 1522, 2405
AflIII ACRYGT 1 cut(s) 351
AgeI ACCGGT 1 cut(s) 1403
AjnI CCWGG 1 cut(s) 526
AjuI GAANNNNNNNTTGG 4 cut(s) 1401, 1433, 2804, 2836
AluBI AGCT 6 cut(s) 156, 723, 1793, 2623, 2730, 2830
AluI AGCT 6 cut(s) 156, 723, 1793, 2623, 2730, 2830
Alw21I GWGCWC 2 cut(s) 2375, 3041
Alw26I GTCTC 5 cut(s) 206, 1164, 1733, 2254, 2420
Alw44I GTGCAC 1 cut(s) 2371
AlwI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2949
AlwNI CAGNNNCTG 2 cut(s) 1472, 1919
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 2 cut(s) 289, 550
AoxI GGCC 7 cut(s) 278, 308, 359, 673, 686, 2229, 2973
ApaLI GTGCAC 1 cut(s) 2371
ApeKI GCWGC 5 cut(s) 63, 168, 1631, 2727, 2827
ApoI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2538, 2856
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 2702, 2734
AseI ATTAAT 1 cut(s) 1302
AsiGI ACCGGT 1 cut(s) 1403
Asp700I GAANNNNTTC 2 cut(s) 839, 3030
AspLEI GCGC 2 cut(s) 89, 381
AspS9I GGNCC 6 cut(s) 202, 309, 360, 441, 484, 2229
AsuC2I CCSGG 4 cut(s) 236, 321, 363, 364
AsuHPI GGTGA 4 cut(s) 177, 335, 1418, 1703
AsuII TTCGAA 2 cut(s) 1051, 1656
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 3 cut(s) 202, 441, 484
BaeGI GKGCMC 1 cut(s) 2375
BaeI ACNNNNGTAYC 2 cut(s) 2448, 2481
BalI TGGCCA 1 cut(s) 675
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 3 cut(s) 321, 1847, 2411
BarI GAAGNNNNNNTAC 2 cut(s) 901, 933
BauI CACGAG 1 cut(s) 823
BbsI GAAGAC 3 cut(s) 2137, 2206, 2687
Bbv12I GWGCWC 2 cut(s) 2375, 3041
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 5 cut(s) 75, 180, 1618, 2739, 2839
BceAI ACGGC 2 cut(s) 181, 490
BcgI CGANNNNNNTGC 4 cut(s) 1375, 1409, 1623, 1657
BciT130I CCWGG 1 cut(s) 528
BclI TGATCA 5 cut(s) 36, 1345, 1668, 2026, 2353
BcnI CCSGG 4 cut(s) 236, 321, 363, 364
BcoDI GTCTC 5 cut(s) 206, 1164, 1733, 2254, 2420
BfaI CTAG 3 cut(s) 1071, 1340, 2804
BfmI CTRYAG 2 cut(s) 1632, 2400
BfuAI ACCTGC 1 cut(s) 2985
BisI GCNGC 7 cut(s) 64, 169, 437, 1632, 2728, 2828, 2973
BlpI GCTNAGC 2 cut(s) 2868, 2919
BlsI GCNGC 7 cut(s) 65, 170, 438, 1633, 2729, 2829, 2974
Bme1390I CCNGG 5 cut(s) 236, 321, 363, 364, 528
Bme18I GGWCC 3 cut(s) 202, 441, 484
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 6 cut(s) 202, 309, 360, 441, 484, 2229
BmiI GGNNCC 5 cut(s) 95, 285, 485, 2230, 2706
BmrFI CCNGG 5 cut(s) 236, 321, 363, 364, 528
BmrI ACTGGG 3 cut(s) 469, 614, 1289
BmsI GCATC 9 cut(s) 730, 1168, 1511, 1762, 2224, 2351, 2554, 2643, 2959
BmuI ACTGGG 3 cut(s) 469, 614, 1289
BpiI GAAGAC 3 cut(s) 2137, 2206, 2687
BplI GAGNNNNNCTC 2 cut(s) 2669, 2701
BpmI CTGGAG 2 cut(s) 1731, 2019
Bpu10I CCTNAGC 1 cut(s) 690
Bpu1102I GCTNAGC 2 cut(s) 2868, 2919
Bpu14I TTCGAA 2 cut(s) 1051, 1656
BpuEI CTTGAG 6 cut(s) 1123, 1707, 1728, 1831, 2395, 2862
BpuMI CCSGG 4 cut(s) 236, 321, 363, 364
Bsa29I ATCGAT 1 cut(s) 1815
BsaAI YACGTR 2 cut(s) 671, 2783
BsaHI GRCGYC 1 cut(s) 336
BsaJI CCNNGG 7 cut(s) 192, 362, 363, 526, 527, 904, 2976
BsaWI WCCGGW 3 cut(s) 289, 550, 1403
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 4 cut(s) 130, 754, 1522, 2405
Bse118I RCCGGY 2 cut(s) 280, 1403
Bse3DI GCAATG 5 cut(s) 1586, 1782, 1812, 1935, 2643
BseAI TCCGGA 2 cut(s) 289, 550
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 1 cut(s) 1815
BseDI CCNNGG 7 cut(s) 192, 362, 363, 526, 527, 904, 2976
BseGI GGATG 5 cut(s) 394, 745, 893, 2569, 2974
BseLI CCNNNNNNNGG 4 cut(s) 130, 754, 1522, 2405
BseMI GCAATG 5 cut(s) 1586, 1782, 1812, 1935, 2643
BseMII CTCAG 6 cut(s) 704, 1092, 1728, 2859, 3026, 3053
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 2375
BseX3I CGGCCG 1 cut(s) 278
BseXI GCAGC 5 cut(s) 75, 180, 1618, 2739, 2839
BseYI CCCAGC 3 cut(s) 1859, 2096, 2419
BsgI GTGCAG 1 cut(s) 1578
Bsh1236I CGCG 2 cut(s) 353, 381
Bsh1285I CGRYCG 2 cut(s) 281, 708
BshFI GGCC 7 cut(s) 280, 310, 361, 675, 688, 2231, 2975
BshNI GGYRCC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 1403
BshVI ATCGAT 1 cut(s) 1815
BsiEI CGRYCG 2 cut(s) 281, 708
BsiHKAI GWGCWC 2 cut(s) 2375, 3041
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
BslFI GGGAC 3 cut(s) 544, 2113, 2182
BslI CCNNNNNNNGG 4 cut(s) 130, 754, 1522, 2405
BsmAI GTCTC 5 cut(s) 206, 1164, 1733, 2254, 2420
BsmFI GGGAC 3 cut(s) 544, 2113, 2182
BsmI GAATGC 5 cut(s) 743, 970, 1219, 2059, 3030
BsnI GGCC 7 cut(s) 280, 310, 361, 675, 688, 2231, 2975
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 2 cut(s) 1051, 1656
Bsp1286I GDGCHC 5 cut(s) 321, 1847, 2375, 2411, 3041
Bsp13I TCCGGA 2 cut(s) 289, 550
Bsp1407I TGTACA 1 cut(s) 1795
Bsp1720I GCTNAGC 2 cut(s) 2868, 2919
Bsp19I CCATGG 1 cut(s) 904
BspANI GGCC 7 cut(s) 280, 310, 361, 675, 688, 2231, 2975
BspCNI CTCAG 6 cut(s) 703, 1091, 1729, 2860, 3027, 3054
BspDI ATCGAT 1 cut(s) 1815
BspEI TCCGGA 2 cut(s) 289, 550
BspFNI CGCG 2 cut(s) 353, 381
BspHI TCATGA 2 cut(s) 2023, 2350
BspLI GGNNCC 5 cut(s) 95, 285, 485, 2230, 2706
BspMAI CTGCAG 1 cut(s) 1636
BspMI ACCTGC 1 cut(s) 2985
BspPI GGATC 5 cut(s) 617, 1082, 1495, 1630, 2949
BspQI GCTCTTC 1 cut(s) 146
BspT104I TTCGAA 2 cut(s) 1051, 1656
BspT107I GGYRCC 1 cut(s) 283
BsrDI GCAATG 5 cut(s) 1586, 1782, 1812, 1935, 2643
BsrFI RCCGGY 2 cut(s) 280, 1403
BsrGI TGTACA 1 cut(s) 1795
BssAI RCCGGY 2 cut(s) 280, 1403
BssECI CCNNGG 7 cut(s) 192, 362, 363, 526, 527, 904, 2976
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 1 cut(s) 823
BssT1I CCWWGG 3 cut(s) 192, 904, 2976
Bst2BI CACGAG 1 cut(s) 823
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 9 cut(s) 400, 650, 1840, 1948, 2043, 2275, 2482, 2504, 2789
Bst6I CTCTTC 1 cut(s) 146
BstACI GRCGYC 1 cut(s) 336
BstAUI TGTACA 1 cut(s) 1795
BstBAI YACGTR 2 cut(s) 671, 2783
BstBI TTCGAA 2 cut(s) 1051, 1656
BstC8I GCNNGC 1 cut(s) 2107
BstDEI CTNAG 9 cut(s) 690, 1078, 1737, 2624, 2741, 2868, 2919, 3035, 3062
BstDSI CCRYGG 1 cut(s) 904
BstEII GGTNACC 1 cut(s) 2156
BstF5I GGATG 5 cut(s) 394, 745, 893, 2569, 2974
BstFNI CGCG 2 cut(s) 353, 381
BstHHI GCGC 2 cut(s) 89, 381
BstMAI GTCTC 5 cut(s) 206, 1164, 1733, 2254, 2420
BstMCI CGRYCG 2 cut(s) 281, 708
BstMWI GCNNNNNNNGC 7 cut(s) 84, 316, 424, 433, 1383, 2102, 3036
BstNI CCWGG 1 cut(s) 528
BstNSI RCATGY 1 cut(s) 1433
BstPI GGTNACC 1 cut(s) 2156
BstSCI CCNGG 5 cut(s) 234, 319, 361, 362, 526
BstSFI CTRYAG 2 cut(s) 1632, 2400
BstSLI GKGCMC 1 cut(s) 2375
BstUI CGCG 2 cut(s) 353, 381
BstV1I GCAGC 5 cut(s) 75, 180, 1618, 2739, 2839
BstV2I GAAGAC 3 cut(s) 2137, 2206, 2687
BstX2I RGATCY 3 cut(s) 1074, 1487, 1622
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 3 cut(s) 1074, 1487, 1622
BstZI CGGCCG 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 1815
BsuRI GGCC 7 cut(s) 280, 310, 361, 675, 688, 2231, 2975
BsuTUI ATCGAT 1 cut(s) 1815
BtgI CCRYGG 1 cut(s) 904
BtsCI GGATG 5 cut(s) 394, 745, 893, 2569, 2974
BtsI GCAGTG 3 cut(s) 1393, 1561, 2262
BveI ACCTGC 1 cut(s) 2985
Cac8I GCNNGC 1 cut(s) 2107
CaiI CAGNNNCTG 2 cut(s) 1472, 1919
CciI TCATGA 2 cut(s) 2023, 2350
CfoI GCGC 2 cut(s) 89, 381
Cfr10I RCCGGY 2 cut(s) 280, 1403
Cfr13I GGNCC 6 cut(s) 202, 309, 360, 441, 484, 2229
Cfr9I CCCGGG 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 1815
CpoI CGGWCCG 1 cut(s) 441
CseI GACGC 2 cut(s) 344, 359
CspAI ACCGGT 1 cut(s) 1403
CspI CGGWCCG 1 cut(s) 441
DdeI CTNAG 9 cut(s) 690, 1078, 1737, 2624, 2741, 2868, 2919, 3035, 3062
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 3 cut(s) 278, 673, 2973
EagI CGGCCG 1 cut(s) 278
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 485
EclXI CGGCCG 1 cut(s) 278
Eco130I CCWWGG 3 cut(s) 192, 904, 2976
Eco24I GRGCYC 3 cut(s) 321, 1847, 2411
Eco32I GATATC 2 cut(s) 1813, 2579
Eco47I GGWCC 3 cut(s) 202, 441, 484
Eco52I CGGCCG 1 cut(s) 278
Eco57I CTGAAG 8 cut(s) 942, 975, 1494, 1518, 1788, 1941, 2294, 2816
Eco88I CYCGRG 1 cut(s) 362
Eco91I GGTNACC 1 cut(s) 2156
EcoO109I RGGNCCY 1 cut(s) 309
EcoO65I GGTNACC 1 cut(s) 2156
EcoRI GAATTC 2 cut(s) 839, 1951
EcoRII CCWGG 1 cut(s) 526
EcoRV GATATC 2 cut(s) 1813, 2579
EcoT14I CCWWGG 3 cut(s) 192, 904, 2976
EcoT22I ATGCAT 3 cut(s) 745, 2061, 2569
EcoT38I GRGCYC 3 cut(s) 321, 1847, 2411
ErhI CCWWGG 3 cut(s) 192, 904, 2976
FaqI GGGAC 3 cut(s) 544, 2113, 2182
FauI CCCGC 2 cut(s) 106, 423
FbaI TGATCA 5 cut(s) 36, 1345, 1668, 2026, 2353
FblI GTMKAC 2 cut(s) 303, 333
Fnu4HI GCNGC 7 cut(s) 64, 169, 437, 1632, 2728, 2828, 2973
FokI GGATG 5 cut(s) 401, 752, 900, 2576, 2981
FriOI GRGCYC 3 cut(s) 321, 1847, 2411
Fsp4HI GCNGC 7 cut(s) 64, 169, 437, 1632, 2728, 2828, 2973
FspBI CTAG 3 cut(s) 1071, 1340, 2804
GlaI GCGC 2 cut(s) 88, 380
GluI GCNGC 7 cut(s) 64, 169, 437, 1632, 2728, 2828, 2973
GsaI CCCAGC 3 cut(s) 1863, 2100, 2423
GsuI CTGGAG 2 cut(s) 1731, 2019
HaeIII GGCC 7 cut(s) 280, 310, 361, 675, 688, 2231, 2975
HapII CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
HgaI GACGC 2 cut(s) 344, 359
HhaI GCGC 2 cut(s) 89, 381
Hin1I GRCGYC 1 cut(s) 336
Hin6I GCGC 2 cut(s) 87, 379
HinP1I GCGC 2 cut(s) 87, 379
HincII GTYRAC 4 cut(s) 304, 334, 602, 2485
HindII GTYRAC 4 cut(s) 304, 334, 602, 2485
HpaI GTTAAC 1 cut(s) 2485
HpaII CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
HphI GGTGA 4 cut(s) 177, 335, 1418, 1703
Hpy166II GTNNAC 8 cut(s) 304, 334, 396, 602, 1043, 2373, 2459, 2485
Hpy8I GTNNAC 8 cut(s) 304, 334, 396, 602, 1043, 2373, 2459, 2485
Hpy99I CGWCG 5 cut(s) 139, 230, 233, 338, 353
HpyAV CCTTC 8 cut(s) 190, 322, 829, 939, 1321, 1559, 1592, 1973
HpyCH4III ACNGT 9 cut(s) 400, 650, 1840, 1948, 2043, 2275, 2482, 2504, 2789
HpyCH4IV ACGT 5 cut(s) 670, 991, 1035, 2756, 2782
HpyF10VI GCNNNNNNNGC 7 cut(s) 84, 316, 424, 433, 1383, 2102, 3036
HpyF3I CTNAG 9 cut(s) 690, 1078, 1737, 2624, 2741, 2868, 2919, 3035, 3062
HpySE526I ACGT 5 cut(s) 670, 991, 1035, 2756, 2782
Hsp92I GRCGYC 1 cut(s) 336
HspAI GCGC 2 cut(s) 87, 379
Kpn2I TCCGGA 2 cut(s) 289, 550
Ksp22I TGATCA 5 cut(s) 36, 1345, 1668, 2026, 2353
KspAI GTTAAC 1 cut(s) 2485
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 2 cut(s) 1518, 2239
Lsp1109I GCAGC 5 cut(s) 75, 180, 1618, 2739, 2839
LweI GCATC 9 cut(s) 730, 1168, 1511, 1762, 2224, 2351, 2554, 2643, 2959
MaeI CTAG 3 cut(s) 1071, 1340, 2804
MaeII ACGT 5 cut(s) 670, 991, 1035, 2756, 2782
MflI RGATCY 3 cut(s) 1074, 1487, 1622
MhlI GDGCHC 5 cut(s) 321, 1847, 2375, 2411, 3041
MlsI TGGCCA 1 cut(s) 675
MluI ACGCGT 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 675
MlyI GAGTC 9 cut(s) 107, 208, 608, 856, 1114, 1223, 1478, 1735, 2164
MmeI TCCRAC 6 cut(s) 249, 708, 748, 1399, 1510, 1659
Mox20I TGGCCA 1 cut(s) 675
Mph1103I ATGCAT 3 cut(s) 745, 2061, 2569
MroI TCCGGA 2 cut(s) 289, 550
MroXI GAANNNNTTC 2 cut(s) 839, 3030
MscI TGGCCA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 1224
Msp20I TGGCCA 1 cut(s) 675
MspI CCGG 9 cut(s) 131, 236, 277, 281, 290, 321, 363, 551, 1404
MspR9I CCNGG 5 cut(s) 236, 321, 363, 364, 528
Mva1269I GAATGC 5 cut(s) 743, 970, 1219, 2059, 3030
MvaI CCWGG 1 cut(s) 528
MvnI CGCG 2 cut(s) 353, 381
MwoI GCNNNNNNNGC 7 cut(s) 84, 316, 424, 433, 1383, 2102, 3036
NciI CCSGG 4 cut(s) 236, 321, 363, 364
NcoI CCATGG 1 cut(s) 904
NlaIV GGNNCC 5 cut(s) 95, 285, 485, 2230, 2706
NmeAIII GCCGAG 1 cut(s) 2449
NmuCI GTSAC 7 cut(s) 808, 1993, 2149, 2218, 2320, 2476, 2783
NsiI ATGCAT 3 cut(s) 745, 2061, 2569
NspI RCATGY 1 cut(s) 1433
NspV TTCGAA 2 cut(s) 1051, 1656
PagI TCATGA 2 cut(s) 2023, 2350
PasI CCCWGGG 1 cut(s) 527
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 300, 321
PctI GAATGC 5 cut(s) 743, 970, 1219, 2059, 3030
PdmI GAANNNNTTC 2 cut(s) 839, 3030
PflFI GACNNNGTC 2 cut(s) 299, 329
PflMI CCANNNNNTGG 1 cut(s) 754
PinAI ACCGGT 1 cut(s) 1403
PkrI GCNGC 7 cut(s) 65, 170, 438, 1633, 2729, 2829, 2974
PleI GAGTC 9 cut(s) 107, 208, 608, 856, 1113, 1223, 1477, 1735, 2164
PpsI GAGTC 9 cut(s) 107, 208, 608, 856, 1113, 1223, 1477, 1735, 2164
Ppu21I YACGTR 2 cut(s) 671, 2783
PshBI ATTAAT 1 cut(s) 1302
Psp1406I AACGTT 1 cut(s) 2756
Psp6I CCWGG 1 cut(s) 526
PspEI GGTNACC 1 cut(s) 2156
PspFI CCCAGC 3 cut(s) 1859, 2096, 2419
PspGI CCWGG 1 cut(s) 526
PspN4I GGNNCC 5 cut(s) 95, 285, 485, 2230, 2706
PspPI GGNCC 6 cut(s) 202, 309, 360, 441, 484, 2229
PstI CTGCAG 1 cut(s) 1636
PstNI CAGNNNCTG 2 cut(s) 1472, 1919
PsuI RGATCY 3 cut(s) 1074, 1487, 1622
PsyI GACNNNGTC 2 cut(s) 299, 329
RseI CAYNNNNRTG 1 cut(s) 1224
Rsr2I CGGWCCG 1 cut(s) 441
RsrII CGGWCCG 1 cut(s) 441
SalI GTCGAC 2 cut(s) 302, 332
SapI GCTCTTC 1 cut(s) 146
SatI GCNGC 7 cut(s) 64, 169, 437, 1632, 2728, 2828, 2973
Sau96I GGNCC 6 cut(s) 202, 309, 360, 441, 484, 2229
SchI GAGTC 9 cut(s) 107, 208, 608, 856, 1114, 1223, 1478, 1735, 2164
ScrFI CCNGG 5 cut(s) 236, 321, 363, 364, 528
SduI GDGCHC 5 cut(s) 321, 1847, 2375, 2411, 3041
SfaNI GCATC 9 cut(s) 730, 1168, 1511, 1762, 2224, 2351, 2554, 2643, 2959
SfcI CTRYAG 2 cut(s) 1632, 2400
SfuI TTCGAA 2 cut(s) 1051, 1656
SinI GGWCC 3 cut(s) 202, 441, 484
SmaI CCCGGG 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 1224
SmlI CTYRAG 6 cut(s) 1102, 1722, 1743, 1846, 2410, 2877
SmoI CTYRAG 6 cut(s) 1102, 1722, 1743, 1846, 2410, 2877
SspMI CTAG 3 cut(s) 1071, 1340, 2804
StyD4I CCNGG 5 cut(s) 234, 319, 361, 362, 526
StyI CCWWGG 3 cut(s) 192, 904, 2976
TaaI ACNGT 9 cut(s) 400, 650, 1840, 1948, 2043, 2275, 2482, 2504, 2789
TaiI ACGT 5 cut(s) 673, 994, 1038, 2759, 2785
TaqII GACCGA 1 cut(s) 722
TatI WGTACW 2 cut(s) 393, 1795
TauI GCSGC 2 cut(s) 439, 2975
TseFI GTSAC 7 cut(s) 808, 1993, 2149, 2218, 2320, 2476, 2783
TseI GCWGC 5 cut(s) 63, 168, 1631, 2727, 2827
Tsp45I GTSAC 7 cut(s) 808, 1993, 2149, 2218, 2320, 2476, 2783
TspDTI ATGAA 8 cut(s) 397, 560, 963, 1014, 1554, 2067, 2825, 2913
TspGWI ACGGA 3 cut(s) 266, 1130, 1505
TspMI CCCGGG 1 cut(s) 362
Tth111I GACNNNGTC 2 cut(s) 299, 329
Van91I CCANNNNNTGG 1 cut(s) 754
VneI GTGCAC 1 cut(s) 2371
VpaK11BI GGWCC 3 cut(s) 202, 441, 484
VspI ATTAAT 1 cut(s) 1302
XapI RAATTY 8 cut(s) 517, 700, 839, 932, 1086, 1951, 2538, 2856
XceI RCATGY 1 cut(s) 1433
XmaI CCCGGG 1 cut(s) 362
XmiI GTMKAC 2 cut(s) 303, 333
XmnI GAANNNNTTC 2 cut(s) 839, 3030
XspI CTAG 3 cut(s) 1071, 1340, 2804
Zsp2I ATGCAT 3 cut(s) 745, 2061, 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.