Rw1G033890

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000527
Physical Location & Seq
Reverse (-)
61848174 .. 61853239
5066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G033890.1

Sequence Viewer

Length: 2163 bp
ATGGCGAAACCGAGCAGCAATACTCGGACTTGGTTCTGGAGCCGAGGCGCGAAAGTGGAGGTGCGCACAGAAGAAGAAGGCTTCCACGGCGCGTGGTTCCCGGCGACGATCCTGGAGCCGCCGTCGAATCCTTCTTCGGTTCTCGGCAGGAAACGAAAGAGCTTCAACTCGAACAAGGTCTTGGTCCAGTACAAGACCTTCGTCACGGAGAAGGACCCAACCAAGCCGCTGACGGAGTACACCAACGTCATGTTCCTCCGGCCGTCGCCGCCGGTGGAGGCTCCCGGGAAGACATTCGAGGCCGGCGAAGACGTGGACGCGTTTCACAACGACGCGTGGGGGGACAGGTACAGGGTGGGGTTTAGGGACCCGCCGGAGTTGCTGGTGCTTGGCCGGAGTGAGTTGAGGAGTCACTTGGATTGGGTCAACGAGATTTGGTCCAGACCATACAAGAAGCGGATGAAGGAATCGATGTATAGTCCGGGGACTCCAATTGAAGTGAGACTTGTCAAAGAACATCTATGGTCTGCTTGGATTCCAGCAATTTATTCTGGTGCATTAGGGGGGAACTTGTTTTTGGTTCAATATAGGAGCCTAAAGAACAGTGATAAAGATGGGATTGCTACAGTGGCTGTATCTGACCATCAGATCCGACCTCGCCCTCCACGGCACAACGAAACAGGTTTTTGCTTGCTGGACATGGTGGATGCGTTCCATGATATGAGTTGGTGGGTTGGAAGTATTACAAATGTTCTTCCAGACAAAAAGTACATTGTCACTTTTAAACTTACAGATGAACAGAAAGAATTCAGTCACTCAGAACTAAGGCTCCATATGGAATGGATTGACAAAAAATGGGAGTCTGACTTTTCGAGATTTGGGTCTGCGATGGATGATATAGAACAAACACAGCATACATTCACAAATTCCAGAAACTCTGCGGTAGCTACACTACTTAAAAGGTCACCTGCTACCAAGGATAGCACGGGAGAAGAAAATCCTTATGCTAGCTCACAGAAGAATCAGATGAAGCAGCTGGGTCCTTGCATTGATAAATCAGCTTATAGGAAAACCACAAATGAACTGAAACGACTGGACCAACCTAATGATGATGCAACATTTTTAAGTCCAACTCCTTCCAAGAAGTTAAAACGACATCCAGAAGAATCTGTTTCTATTGATGAAGTATATGTGAGGAGAACACCAATTAAAATATCCAATGAAAAAGACCCTGTTGGAGATGGTGTGAAGACTGGGTCGCTGCTTGGAGAACCAGGTAGACAAAGAAAATCACAATTTGATGGGAGCAGTCCGCCACTGAAAATTCATGTTGCAGTCAAGAAGGGAAGTAGAATGGACAAGGCTGATGAAGAATGTATTGGCGATGAGCATGTAATGGACAAGACTGAATCTTCTGGTATTCAGTCAGACTCTGAAGTAACGAGAAGATCACTTGCTGGAAATTATAGTCTCCTGCATTTGAAGGAGATTAATCAACATGATGAAGATGGAGTGAGCAATCAGACAAAAGTGAAGGATTCTGAAGATTCAACAAGAAATGGGACAACAGAAGCATCTGGAATGGAAGTTCAGGTGACTGTGCCTGGATTGTCTGCAAAGCCTGCTGCTCATGATCAGCCAGTATCATTGTGTACAGATGAAATGCCTTCTGAATACACCATTGAGTGCTCAGTTAATCCGGTTGATGAAATGCATTCTCAATGCACTCTTAATCAGGATGGCAAAATGTTTCATGAAGGCACTATGGAGTGTGCAGGTTCTGAAGATCCAACAGAAGATGGAAAAGCTCAAGCTACTGCAATGGAAGTAGAGGTGGCTGGGACTGAAATATCCTCAGAGGCACCTCTTCCTGATCAACCTTTATTATTGTGTGACATGGACTCTGAAAACACTTTGGAGGGTAACCCCGCTGGGACTGCAAGAACAACCCTTCATGATCAATTTCAGGCATCAAGCATAGCTGAAATGTATTCTGAACATACTACTGAGGGCTCATACCTCACTTCATGTTGTCTGACAAAATTGAATGGAAAATGTGCTCTAGGCAAGCGTGCTGGGACAACAAAGCTTACTGGGAGACAAGGTGAAGCTGGAATTACCAGTGAATTGAGCAAGAAGACACTCCACACCTGCCTTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

720

Amino Acids

80.19

Weight (kDa)

5.65

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 91 7.2e-14 Agenet domain
Agenet PF05641 162 - 222 1.9e-07 Agenet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 976, 2159
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 3 cut(s) 976, 1766, 2159
AccB1I GGYRCC 1 cut(s) 1861
AccI GTMKAC 1 cut(s) 1279
AccII CGCG 4 cut(s) 50, 92, 320, 335
AciI CCGC 8 cut(s) 119, 227, 269, 371, 457, 941, 1313, 1929
AclWI GGATC 3 cut(s) 103, 643, 1781
AcoI YGGCCR 2 cut(s) 260, 391
AcsI RAATTY 3 cut(s) 806, 925, 1323
AcuI CTGAAG 3 cut(s) 1455, 1563, 1803
AfaI GTAC 5 cut(s) 191, 239, 350, 770, 1654
AflIII ACRYGT 2 cut(s) 318, 333
AgsI TTSAA 6 cut(s) 166, 497, 584, 1483, 1551, 2047
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 3 cut(s) 111, 1273, 1603
AjuI GAANNNNNNNTTGG 8 cut(s) 164, 196, 236, 268, 560, 592, 1362, 1394
AloI GAACNNNNNNTCC 4 cut(s) 813, 845, 1572, 1604
Alw21I GWGCWC 2 cut(s) 1691, 2062
Alw26I GTCTC 3 cut(s) 496, 1475, 2092
AlwI GGATC 3 cut(s) 103, 643, 1781
AlwNI CAGNNNCTG 3 cut(s) 632, 1433, 1781
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 3 cut(s) 260, 300, 391
ApeKI GCWGC 4 cut(s) 15, 1033, 1261, 1625
ApoI RAATTY 3 cut(s) 806, 925, 1323
AseI ATTAAT 1 cut(s) 1491
Asp700I GAANNNNTTC 2 cut(s) 293, 806
AspLEI GCGC 3 cut(s) 50, 66, 92
AspS9I GGNCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
AsuC2I CCSGG 4 cut(s) 101, 285, 286, 483
AsuHPI GGTGA 3 cut(s) 957, 1606, 2117
AsuNHI GCTAGC 1 cut(s) 1007
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
BanI GGYRCC 1 cut(s) 1861
BanII GRGCYC 1 cut(s) 2015
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbsI GAAGAC 4 cut(s) 296, 315, 1256, 2144
Bbv12I GWGCWC 2 cut(s) 1691, 2062
BbvI GCAGC 4 cut(s) 27, 1045, 1248, 1612
BccI CCATC 8 cut(s) 608, 651, 883, 1235, 1295, 1502, 1733, 1793
BceAI ACGGC 4 cut(s) 103, 106, 247, 683
BciT130I CCWGG 3 cut(s) 113, 1275, 1605
BclI TGATCA 3 cut(s) 1633, 1873, 1957
BcnI CCSGG 4 cut(s) 101, 285, 286, 483
BcoDI GTCTC 3 cut(s) 496, 1475, 2092
BfaI CTAG 2 cut(s) 1008, 2063
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 3 cut(s) 976, 1766, 2159
BisI GCNGC 7 cut(s) 16, 119, 227, 269, 1034, 1262, 1626
BlsI GCNGC 7 cut(s) 17, 120, 228, 270, 1035, 1263, 1627
Bme1390I CCNGG 7 cut(s) 101, 113, 285, 286, 483, 1275, 1605
Bme18I GGWCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
BmrFI CCNGG 7 cut(s) 101, 113, 285, 286, 483, 1275, 1605
BmrI ACTGGG 2 cut(s) 1263, 2103
BmsI GCATC 4 cut(s) 697, 1102, 1583, 1979
BmtI GCTAGC 1 cut(s) 1011
BmuI ACTGGG 2 cut(s) 1263, 2103
BoxI GACNNNNGTC 1 cut(s) 200
BpiI GAAGAC 4 cut(s) 296, 315, 1256, 2144
BpmI CTGGAG 2 cut(s) 58, 134
BpuEI CTTGAG 1 cut(s) 1794
BpuMI CCSGG 4 cut(s) 101, 285, 286, 483
Bsa29I ATCGAT 1 cut(s) 470
BsaJI CCNNGG 6 cut(s) 43, 85, 284, 482, 665, 975
BsaWI WCCGGW 1 cut(s) 1699
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 813, 843, 1230, 1260, 2089, 2119
Bse118I RCCGGY 2 cut(s) 271, 302
Bse1I ACTGG 6 cut(s) 187, 1098, 1258, 1640, 2098, 2121
Bse3DI GCAATG 1 cut(s) 1827
BseBI CCWGG 3 cut(s) 113, 1275, 1605
BseCI ATCGAT 1 cut(s) 470
BseDI CCNNGG 6 cut(s) 43, 85, 284, 482, 665, 975
BseGI GGATG 5 cut(s) 465, 712, 898, 1156, 1744
BseMI GCAATG 1 cut(s) 1827
BseMII CTCAG 4 cut(s) 831, 1704, 1869, 1998
BseNI ACTGG 6 cut(s) 187, 1098, 1258, 1640, 2098, 2121
BseRI GAGGAG 2 cut(s) 421, 1210
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 4 cut(s) 27, 1045, 1248, 1612
BseYI CCCAGC 4 cut(s) 1036, 1838, 1931, 2075
BsgI GTGCAG 1 cut(s) 1794
Bsh1236I CGCG 4 cut(s) 50, 92, 320, 335
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 3 cut(s) 262, 302, 393
BshNI GGYRCC 1 cut(s) 1861
BshVI ATCGAT 1 cut(s) 470
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 1691, 2062
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 9 cut(s) 101, 259, 272, 285, 303, 374, 394, 482, 1700
BslFI GGGAC 7 cut(s) 356, 380, 499, 1576, 1855, 1948, 2092
BsmAI GTCTC 3 cut(s) 496, 1475, 2092
BsmFI GGGAC 7 cut(s) 356, 380, 499, 1576, 1855, 1948, 2092
BsmI GAATGC 1 cut(s) 1714
BsnI GGCC 3 cut(s) 262, 302, 393
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 1691, 2015, 2062
Bsp1407I TGTACA 1 cut(s) 1652
Bsp143I GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
BspACI CCGC 8 cut(s) 119, 227, 269, 371, 457, 941, 1313, 1929
BspANI GGCC 3 cut(s) 262, 302, 393
BspCNI CTCAG 4 cut(s) 830, 1703, 1868, 1999
BspDI ATCGAT 1 cut(s) 470
BspFNI CGCG 4 cut(s) 50, 92, 320, 335
BspHI TCATGA 3 cut(s) 1630, 1753, 1954
BspMI ACCTGC 3 cut(s) 976, 1766, 2159
BspOI GCTAGC 1 cut(s) 1011
BspPI GGATC 3 cut(s) 103, 643, 1781
BspT107I GGYRCC 1 cut(s) 1861
BsrDI GCAATG 1 cut(s) 1827
BsrFI RCCGGY 2 cut(s) 271, 302
BsrGI TGTACA 1 cut(s) 1652
BsrI ACTGG 6 cut(s) 187, 1098, 1258, 1640, 2098, 2121
BssAI RCCGGY 2 cut(s) 271, 302
BssECI CCNNGG 6 cut(s) 43, 85, 284, 482, 665, 975
BssMI GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 3 cut(s) 113, 1275, 1605
Bst4CI ACNGT 3 cut(s) 605, 628, 1600
Bst6I CTCTTC 1 cut(s) 1872
BstAPI GCANNNNNTGC 1 cut(s) 1622
BstAUI TGTACA 1 cut(s) 1652
BstC8I GCNNGC 6 cut(s) 304, 692, 1009, 1623, 2069, 2073
BstDEI CTNAG 5 cut(s) 817, 824, 1690, 1855, 2007
BstDSI CCRYGG 2 cut(s) 85, 665
BstEII GGTNACC 2 cut(s) 963, 1922
BstF5I GGATG 5 cut(s) 465, 712, 898, 1156, 1744
BstFNI CGCG 4 cut(s) 50, 92, 320, 335
BstHHI GCGC 3 cut(s) 50, 66, 92
BstKTI GATC 7 cut(s) 111, 651, 1451, 1636, 1789, 1876, 1960
BstMAI GTCTC 3 cut(s) 496, 1475, 2092
BstMBI GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 6 cut(s) 87, 268, 379, 629, 1622, 1937
BstNI CCWGG 3 cut(s) 113, 1275, 1605
BstNSI RCATGY 1 cut(s) 1394
BstPAI GACNNNNGTC 1 cut(s) 200
BstPI GGTNACC 2 cut(s) 963, 1922
BstSCI CCNGG 7 cut(s) 99, 111, 283, 284, 481, 1273, 1603
BstSFI CTRYAG 1 cut(s) 624
BstUI CGCG 4 cut(s) 50, 92, 320, 335
BstV1I GCAGC 4 cut(s) 27, 1045, 1248, 1612
BstV2I GAAGAC 4 cut(s) 296, 315, 1256, 2144
BstX2I RGATCY 2 cut(s) 648, 1786
BstYI RGATCY 2 cut(s) 648, 1786
BstZI CGGCCG 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 470
BsuRI GGCC 3 cut(s) 262, 302, 393
BsuTUI ATCGAT 1 cut(s) 470
BtgI CCRYGG 2 cut(s) 85, 665
BtgZI GCGATG 2 cut(s) 902, 1398
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 5 cut(s) 465, 712, 898, 1156, 1744
BtsIMutI CAGTG 4 cut(s) 610, 633, 1316, 2128
BveI ACCTGC 3 cut(s) 976, 1766, 2159
Cac8I GCNNGC 6 cut(s) 304, 692, 1009, 1623, 2069, 2073
CaiI CAGNNNCTG 3 cut(s) 632, 1433, 1781
CciI TCATGA 3 cut(s) 1630, 1753, 1954
CfoI GCGC 3 cut(s) 50, 66, 92
Cfr10I RCCGGY 2 cut(s) 271, 302
Cfr13I GGNCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
Cfr9I CCCGGG 1 cut(s) 284
ClaI ATCGAT 1 cut(s) 470
CseI GACGC 2 cut(s) 326, 341
CsiI ACCWGGT 1 cut(s) 1273
Csp6I GTAC 5 cut(s) 190, 238, 349, 769, 1653
CviQI GTAC 5 cut(s) 190, 238, 349, 769, 1653
DdeI CTNAG 5 cut(s) 817, 824, 1690, 1855, 2007
DpnI GATC 7 cut(s) 110, 650, 1450, 1635, 1788, 1875, 1959
DpnII GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
DraI TTTAAA 1 cut(s) 784
EaeI YGGCCR 2 cut(s) 260, 391
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 1872
EarI CTCTTC 1 cut(s) 1872
EciI GGCGGA 1 cut(s) 1302
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 975
Eco24I GRGCYC 1 cut(s) 2015
Eco47I GGWCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 3 cut(s) 1455, 1563, 1803
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 2 cut(s) 963, 1922
EcoO109I RGGNCCY 3 cut(s) 214, 367, 1040
EcoO65I GGTNACC 2 cut(s) 963, 1922
EcoRI GAATTC 1 cut(s) 806
EcoRII CCWGG 3 cut(s) 111, 1273, 1603
EcoT14I CCWWGG 1 cut(s) 975
EcoT22I ATGCAT 1 cut(s) 1716
EcoT38I GRGCYC 1 cut(s) 2015
ErhI CCWWGG 1 cut(s) 975
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 7 cut(s) 356, 380, 499, 1576, 1855, 1948, 2092
FauI CCCGC 2 cut(s) 378, 1936
FauNDI CATATG 1 cut(s) 834
FbaI TGATCA 3 cut(s) 1633, 1873, 1957
FblI GTMKAC 1 cut(s) 1279
Fnu4HI GCNGC 7 cut(s) 16, 119, 227, 269, 1034, 1262, 1626
FokI GGATG 5 cut(s) 472, 719, 905, 1143, 1751
FriOI GRGCYC 1 cut(s) 2015
Fsp4HI GCNGC 7 cut(s) 16, 119, 227, 269, 1034, 1262, 1626
FspAI RTGCGCAY 1 cut(s) 65
FspBI CTAG 2 cut(s) 1008, 2063
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 3 cut(s) 49, 65, 91
GluI GCNGC 7 cut(s) 16, 119, 227, 269, 1034, 1262, 1626
GsaI CCCAGC 4 cut(s) 1040, 1842, 1935, 2079
GsuI CTGGAG 2 cut(s) 58, 134
HaeIII GGCC 3 cut(s) 262, 302, 393
HapII CCGG 9 cut(s) 101, 259, 272, 285, 303, 374, 394, 482, 1700
HgaI GACGC 2 cut(s) 326, 341
HhaI GCGC 3 cut(s) 50, 66, 92
Hin6I GCGC 3 cut(s) 48, 64, 90
HinP1I GCGC 3 cut(s) 48, 64, 90
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HindIII AAGCTT 1 cut(s) 2087
HpaII CCGG 9 cut(s) 101, 259, 272, 285, 303, 374, 394, 482, 1700
HphI GGTGA 3 cut(s) 957, 1606, 2117
Hpy166II GTNNAC 5 cut(s) 240, 316, 427, 1280, 1653
Hpy8I GTNNAC 5 cut(s) 240, 316, 427, 1280, 1653
Hpy99I CGWCG 4 cut(s) 109, 127, 268, 335
HpyCH4III ACNGT 3 cut(s) 605, 628, 1600
HpyCH4IV ACGT 2 cut(s) 246, 312
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 268, 379, 629, 1622, 1937
HpyF3I CTNAG 5 cut(s) 817, 824, 1690, 1855, 2007
HpySE526I ACGT 2 cut(s) 246, 312
HspAI GCGC 3 cut(s) 48, 64, 90
KflI GGGWCCC 1 cut(s) 367
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 3 cut(s) 1633, 1873, 1957
Kzo9I GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
LmnI GCTCC 6 cut(s) 39, 115, 286, 591, 834, 1305
Lsp1109I GCAGC 4 cut(s) 27, 1045, 1248, 1612
LweI GCATC 4 cut(s) 697, 1102, 1583, 1979
MabI ACCWGGT 1 cut(s) 1273
MaeI CTAG 2 cut(s) 1008, 2063
MaeII ACGT 2 cut(s) 246, 312
MaeIII GTNAC 9 cut(s) 202, 410, 775, 812, 963, 1438, 1594, 1892, 1922
MalI GATC 7 cut(s) 110, 650, 1450, 1635, 1788, 1875, 1959
MboI GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
MfeI CAATTG 1 cut(s) 492
MflI RGATCY 2 cut(s) 648, 1786
MhlI GDGCHC 3 cut(s) 1691, 2015, 2062
MluI ACGCGT 2 cut(s) 318, 333
MlyI GAGTC 5 cut(s) 418, 481, 869, 1424, 1895
MmeI TCCRAC 5 cut(s) 676, 715, 1154, 1216, 1814
Mph1103I ATGCAT 1 cut(s) 1716
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 2 cut(s) 293, 806
MseI TTAA 8 cut(s) 783, 957, 1124, 1148, 1209, 1491, 1695, 1731
MspA1I CMGCKG 3 cut(s) 229, 1036, 1931
MspI CCGG 9 cut(s) 101, 259, 272, 285, 303, 374, 394, 482, 1700
MspR9I CCNGG 7 cut(s) 101, 113, 285, 286, 483, 1275, 1605
MunI CAATTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 1714
MvaI CCWGG 3 cut(s) 113, 1275, 1605
MvnI CGCG 4 cut(s) 50, 92, 320, 335
MwoI GCNNNNNNNGC 6 cut(s) 87, 268, 379, 629, 1622, 1937
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 4 cut(s) 101, 285, 286, 483
NdeI CATATG 1 cut(s) 834
NdeII GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
NgoMIV GCCGGC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 1007
NmeAIII GCCGAG 2 cut(s) 68, 123
NmuCI GTSAC 7 cut(s) 202, 410, 775, 812, 963, 1594, 1892
NsbI TGCGCA 1 cut(s) 65
NsiI ATGCAT 1 cut(s) 1716
NspI RCATGY 1 cut(s) 1394
PagI TCATGA 3 cut(s) 1630, 1753, 1954
PaqCI CACCTGC 2 cut(s) 976, 2159
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 1714
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 2 cut(s) 293, 806
PfeI GAWTC 8 cut(s) 127, 467, 535, 1021, 1166, 1409, 1538, 1547
PflFI GACNNNGTC 1 cut(s) 1255
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 7 cut(s) 17, 120, 228, 270, 1035, 1263, 1627
PleI GAGTC 5 cut(s) 417, 481, 868, 1424, 1895
PpsI GAGTC 5 cut(s) 417, 481, 868, 1424, 1895
PpuMI RGGWCCY 3 cut(s) 214, 367, 1040
PshAI GACNNNNGTC 1 cut(s) 200
PshBI ATTAAT 1 cut(s) 1491
Psp5II RGGWCCY 3 cut(s) 214, 367, 1040
Psp6I CCWGG 3 cut(s) 111, 1273, 1603
PspEI GGTNACC 2 cut(s) 963, 1922
PspFI CCCAGC 4 cut(s) 1036, 1838, 1931, 2075
PspGI CCWGG 3 cut(s) 111, 1273, 1603
PspPI GGNCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
PspPPI RGGWCCY 3 cut(s) 214, 367, 1040
PstNI CAGNNNCTG 3 cut(s) 632, 1433, 1781
PsuI RGATCY 2 cut(s) 648, 1786
PsyI GACNNNGTC 1 cut(s) 1255
PvuII CAGCTG 1 cut(s) 1036
RsaI GTAC 5 cut(s) 191, 239, 350, 770, 1654
RsaNI GTAC 5 cut(s) 190, 238, 349, 769, 1653
SaqAI TTAA 8 cut(s) 783, 957, 1124, 1148, 1209, 1491, 1695, 1731
SatI GCNGC 7 cut(s) 16, 119, 227, 269, 1034, 1262, 1626
Sau3AI GATC 7 cut(s) 108, 648, 1448, 1633, 1786, 1873, 1957
Sau96I GGNCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
SchI GAGTC 5 cut(s) 418, 481, 869, 1424, 1895
ScrFI CCNGG 7 cut(s) 101, 113, 285, 286, 483, 1275, 1605
SduI GDGCHC 3 cut(s) 1691, 2015, 2062
SexAI ACCWGGT 1 cut(s) 1273
SfaNI GCATC 4 cut(s) 697, 1102, 1583, 1979
SfcI CTRYAG 1 cut(s) 624
SgrAI CRCCGGYG 1 cut(s) 271
SinI GGWCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
SmaI CCCGGG 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 1809
SmoI CTYRAG 1 cut(s) 1809
SsiI CCGC 8 cut(s) 119, 227, 269, 371, 457, 941, 1313, 1929
SspMI CTAG 2 cut(s) 1008, 2063
StyD4I CCNGG 7 cut(s) 99, 111, 283, 284, 481, 1273, 1603
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 3 cut(s) 605, 628, 1600
TaiI ACGT 2 cut(s) 249, 315
TaqI TCGA 5 cut(s) 125, 170, 297, 470, 872
TatI WGTACW 4 cut(s) 189, 237, 768, 1652
TauI GCSGC 3 cut(s) 121, 229, 271
TfiI GAWTC 8 cut(s) 127, 467, 535, 1021, 1166, 1409, 1538, 1547
Tru1I TTAA 8 cut(s) 783, 957, 1124, 1148, 1209, 1491, 1695, 1731
Tru9I TTAA 8 cut(s) 783, 957, 1124, 1148, 1209, 1491, 1695, 1731
TscAI CASTG 4 cut(s) 610, 633, 1323, 2128
TseFI GTSAC 7 cut(s) 202, 410, 775, 812, 963, 1594, 1892
TseI GCWGC 4 cut(s) 15, 1033, 1261, 1625
Tsp45I GTSAC 7 cut(s) 202, 410, 775, 812, 963, 1594, 1892
TspGWI ACGGA 2 cut(s) 221, 248
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 4 cut(s) 610, 633, 1323, 2128
Tth111I GACNNNGTC 1 cut(s) 1255
VpaK11BI GGWCC 6 cut(s) 184, 214, 367, 438, 1040, 1096
VspI ATTAAT 1 cut(s) 1491
XapI RAATTY 3 cut(s) 806, 925, 1323
XceI RCATGY 1 cut(s) 1394
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1279
XmnI GAANNNNTTC 2 cut(s) 293, 806
XspI CTAG 2 cut(s) 1008, 2063
Zsp2I ATGCAT 1 cut(s) 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.