Rh1AG384200

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000527
Physical Location & Seq
Reverse (-)
61536750 .. 61540894
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG384200.1

Sequence Viewer

Length: 2409 bp
ATGAAGAGCCCGAAGGAGGAGATCAGTTGTCCCCCATGTGAGTATTTCAGCCGCGGCGCGAAAGTGGAGGTGCGCGACGAGCAAGGTGCGTGGTTTCCTGCTCATGTCCTGGACCCAAATCCAAACCAGTCCCCATTCTCGCTTCGGAATCGGAATCGGAAGCAGAAGTTGCTGGTGGTAGAGTATGAAACTCTCGTCTCCGACGACGACCCTTCGAAGCCGCTGACGGAGGAAATCGAGGTGGGTATGCTCCGGCCGTCGCCGCCTGCGGAGGAGGAGGAGGAGGGGTTTGAGGCAAACGACGTCGTTGACGCGTTTAATTTGGACGCGTGGTGGCCCGGGGTGGTGATGAGTGTTGTGGGGGACAAGTACACGGTGGGGTTTAAGAGCCCGCCGGATTTGCTGGAGCTTGGGCGCGGCGAGCTGCGGCGCCATTGGGATTGGCGCGGCGGCGAGTGGACTCGAGCCGGGAAGGAGATGATGATGCGATTGGATTTCAGCCCTGGGGAAGATGTTGAAGTGAACCTACACAAAGAACGTGTATGGTATGCTTGGATACCGGCAATTTATGTTGGTCCATTAGGGGGCAATTCTTTCCTGGTCCGTTATAAGGGTGCGAATAATGGTCATGAAAATGGTTTTGTTAAAGTTGTATCGGACTATCAGATTCGACCTCTTCCTCCGCAGCAAGAGGAAAGAGCTTTTGAATTGTTGGGAATGGTGGATGCATACCTAGATATGTGTTGGTGGGTTGGAGACATTACAAAAGTTCTTACAGGGGAAAGGTATGTTGTCACATTAAGATTCACAAAGCAGGAGAAGGAATTCAGATCGTCAGACCTGAGGCACCACTCAGAATGGATTGGTAGCAAATGGGTGTCTGACCCTAAGGCACTTGAAAATTCTATTGTCAATGAAGAACGATTAAGATATGCAAGAAACAATTACAGAAAATCCGAAGAAAAAACACCTTGTCATACTAAGTCTCAAATGAAGCAGTTGACTTGTCTTGTGAAGAAATCACCTTACGGAAATACCATTAAGAAACTAAAATTATCTGAGCTTTCTAATGATGGTGCAACATTTTCAAGTTCAACTCCTTCAAAGAAGTTAAAAGGGGAACGCTCAGAAGACTCCTTATCTCTGTCAGCATTATTTGGCAGGGCAAAATCAGTTAAAACATCCAACAGAAAAGGGCCTTTTGAAGATGGAGAGAAGTCCGAGCAACAACTTGTCGGGCAACTGGATGGTGAAGTAACCACTCCCGTAAAAAGAAGGGTTGGACAACGAAAATCACAAGCTGATAGCCCTTCCCCTGGTGTTAGACAGGGTACAAAGGCTAGACGACCAAAATTGCATGTCAAGAAGAGAAGTGGAATTGACAAAGCATATGAACAAAATCTTGAAGATGAGCATGTAATTAACGGAATTGAATCTTCTGGGATCGGTTCGGAGAATGAAATAATGGGGGGATCAAAGGCTGGAAGCTCATGTCTGCTCCCTATGGAGGATAACCAACAACATAATGAAGATGGAGCAAGCAGTGGAATAAAACTGAAGAGAAAACTGCCTATGTTAGCTGTTGAACACTCAAAAGATCCAACAACAGGTTCTGGAGATTCGGTGAGAGATGGCCGAGCTGTAGCTTCTGTAGAGAAAGTTAAGCTGGCTGTGTCTGGAATTCCTGCAAAGAAACTTGATCATGATCAATCTTTATTCATAAAGGAAGGGAATTCTGTAAAAACCGTGGAGGGATCAATAAGTGGAAGAAAAATTGAAACTGGAGTCAAAGGTGATGTGGCGCAACAAGACAGTCCAAATTTGCCTTTTGTGAAAAGTATCCCACTTTGGGAAAATATTGAATCCATGGAAGTATTCAAAAGATTTCCACAGAAGCCGCATTTTCATCCTGTACTGAAATCTAAAGAGGTGTTTCGTGAGGGATCGGCTTTCGGAAAAATGTTAGCCTTTCGCACTCTGGTAGACTGGACCTCCAAGATACGGATTGGTGATCCTAGAAACGTCATTGATAGCAACTTGGAAGCGACAGTTGAATTGGAACTGCACGGCTTTGATGTTGATGCCATTAAGTGTCGTCTGACTGGATTGCTAGACCTGAAAGCCAAGCTGGAGGAGCTTCAAAACCAGTCCAAAGTAGTTGAAACCAAGATCACGGCGTGTAGACAAAACACAATCAAGGACACTGAAACTATCTGTAGAATTGACAAGGAGATCAAGGACTTAGAAGCAAACATCAAGGGCTTGAAGAAAAAACGGGCCATGGCTGTGTCAATGTGTTCGGCCAAAGATTCCGAGCTTAGCAAGTTGCAGTCAGAAGCAAAAGTTATCAGTGAAGGCATTCAGAGCATCCAACATGGTTTCGACATACTAGCTGCTGCTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

802

Amino Acids

89.8

Weight (kDa)

8.1

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 19 - 89 7.4e-10 Agenet domain
Agenet PF05641 169 - 228 3e-06 Agenet domain
DUF724 PF05266 608 - 801 3.1e-51 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 609
AatII GACGTC 1 cut(s) 306
AccB1I GGYRCC 2 cut(s) 429, 846
AccI GTMKAC 2 cut(s) 1983, 2182
AccII CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
AclWI GGATC 6 cut(s) 1451, 1480, 1592, 1762, 1951, 2006
AcoI YGGCCR 3 cut(s) 254, 1633, 2301
AcsI RAATTY 5 cut(s) 824, 901, 1680, 1732, 1819
AcuI CTGAAG 1 cut(s) 1577
AcyI GRCGYC 2 cut(s) 303, 430
AdeI CACNNNGTG 1 cut(s) 2178
AfaI GTAC 3 cut(s) 371, 1333, 1914
AfiI CCNNNNNNNGG 8 cut(s) 16, 584, 610, 1316, 1507, 1607, 1849, 2001
AflIII ACRYGT 3 cut(s) 312, 327, 538
AjnI CCWGG 4 cut(s) 108, 502, 597, 1315
AjuI GAANNNNNNNTTGG 2 cut(s) 1594, 1626
Alw26I GTCTC 3 cut(s) 202, 750, 990
AlwI GGATC 6 cut(s) 1451, 1480, 1592, 1762, 1951, 2006
AlwNI CAGNNNCTG 1 cut(s) 1613
Ama87I CYCGRG 2 cut(s) 338, 462
AoxI GGCC 6 cut(s) 254, 335, 1196, 1633, 2277, 2301
ApeKI GCWGC 4 cut(s) 424, 685, 2393, 2396
ApoI RAATTY 5 cut(s) 824, 901, 1680, 1732, 1819
Asp700I GAANNNNTTC 2 cut(s) 824, 2358
AspLEI GCGC 6 cut(s) 59, 75, 417, 432, 447, 1804
AspS9I GGNCC 7 cut(s) 112, 336, 575, 601, 1196, 1989, 2277
AsuC2I CCSGG 3 cut(s) 339, 340, 469
AsuHPI GGTGA 6 cut(s) 358, 1014, 1262, 1636, 1805, 2021
AsuII TTCGAA 1 cut(s) 215
AvaI CYCGRG 2 cut(s) 338, 462
AvaII GGWCC 4 cut(s) 112, 575, 601, 1989
AxyI CCTNAGG 2 cut(s) 842, 888
BanI GGYRCC 2 cut(s) 429, 846
BanII GRGCYC 2 cut(s) 11, 392
BarI GAAGNNNNNNTAC 2 cut(s) 510, 542
BbsI GAAGAC 1 cut(s) 1137
BbvI GCAGC 4 cut(s) 411, 697, 2380, 2383
BccI CCATC 5 cut(s) 1067, 1202, 1241, 1526, 1625
BceAI ACGGC 3 cut(s) 241, 2083, 2190
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 110, 504, 599, 1317
BciVI GTATCC 2 cut(s) 549, 1850
BclI TGATCA 2 cut(s) 1699, 1705
BcnI CCSGG 3 cut(s) 339, 340, 469
BcoDI GTCTC 3 cut(s) 202, 750, 990
BfaI CTAG 5 cut(s) 734, 1341, 2016, 2111, 2390
BfmI CTRYAG 3 cut(s) 1641, 1650, 2215
BfoI RGCGCY 1 cut(s) 433
BfuI GTATCC 2 cut(s) 549, 1850
BlpI GCTNAGC 1 cut(s) 2318
Bme1390I CCNGG 7 cut(s) 110, 339, 340, 469, 504, 599, 1317
Bme18I GGWCC 4 cut(s) 112, 575, 601, 1989
BmeT110I CYCGRG 2 cut(s) 338, 462
BmgT120I GGNCC 7 cut(s) 112, 336, 575, 601, 1196, 1989, 2277
BmiI GGNNCC 3 cut(s) 114, 431, 848
BmrFI CCNGG 7 cut(s) 110, 339, 340, 469, 504, 599, 1317
BmsI GCATC 4 cut(s) 474, 715, 2071, 2376
BpiI GAAGAC 1 cut(s) 1137
BpmI CTGGAG 4 cut(s) 425, 1635, 1803, 2150
Bpu1102I GCTNAGC 1 cut(s) 2318
Bpu14I TTCGAA 1 cut(s) 215
BpuMI CCSGG 3 cut(s) 339, 340, 469
BsaBI GATNNNNATC 1 cut(s) 1704
BsaHI GRCGYC 2 cut(s) 303, 430
BsaJI CCNNGG 9 cut(s) 52, 338, 339, 502, 503, 1315, 1746, 1866, 2280
BsaXI ACNNNNNCTCC 6 cut(s) 272, 302, 1608, 1638, 1976, 2006
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 584, 610, 1316, 1507, 1607, 1849, 2001
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 6 cut(s) 127, 1248, 1786, 1991, 2107, 2146
Bse21I CCTNAGG 2 cut(s) 842, 888
Bse8I GATNNNNATC 1 cut(s) 1704
BseBI CCWGG 4 cut(s) 110, 504, 599, 1317
BseDI CCNNGG 9 cut(s) 52, 338, 339, 502, 503, 1315, 1746, 1866, 2280
BseGI GGATG 5 cut(s) 730, 1181, 1252, 1906, 2367
BseJI GATNNNNATC 1 cut(s) 1704
BseLI CCNNNNNNNGG 8 cut(s) 16, 584, 610, 1316, 1507, 1607, 1849, 2001
BseMII CTCAG 4 cut(s) 833, 867, 1050, 1140
BseNI ACTGG 6 cut(s) 127, 1248, 1786, 1991, 2107, 2146
BseRI GAGGAG 6 cut(s) 32, 287, 290, 293, 296, 2147
BseX3I CGGCCG 1 cut(s) 254
BseXI GCAGC 4 cut(s) 411, 697, 2380, 2383
BsgI GTGCAG 1 cut(s) 2048
Bsh1236I CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
Bsh1285I CGRYCG 1 cut(s) 257
BshFI GGCC 6 cut(s) 256, 337, 1198, 1635, 2279, 2303
BshNI GGYRCC 2 cut(s) 429, 846
BsiEI CGRYCG 1 cut(s) 257
BsiHKCI CYCGRG 2 cut(s) 338, 462
BsiSI CCGG 5 cut(s) 253, 339, 395, 468, 560
BslFI GGGAC 3 cut(s) 15, 115, 377
BslI CCNNNNNNNGG 8 cut(s) 16, 584, 610, 1316, 1507, 1607, 1849, 2001
BsmAI GTCTC 3 cut(s) 202, 750, 990
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 3 cut(s) 15, 115, 377
BsmI GAATGC 1 cut(s) 2358
BsnI GGCC 6 cut(s) 256, 337, 1198, 1635, 2279, 2303
BsoBI CYCGRG 2 cut(s) 338, 462
Bsp119I TTCGAA 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 11, 392
Bsp1720I GCTNAGC 1 cut(s) 2318
Bsp19I CCATGG 2 cut(s) 1866, 2280
BspANI GGCC 6 cut(s) 256, 337, 1198, 1635, 2279, 2303
BspCNI CTCAG 4 cut(s) 834, 866, 1051, 1139
BspFNI CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
BspHI TCATGA 2 cut(s) 628, 1702
BspLI GGNNCC 3 cut(s) 114, 431, 848
BspPI GGATC 6 cut(s) 1451, 1480, 1592, 1762, 1951, 2006
BspT104I TTCGAA 1 cut(s) 215
BspT107I GGYRCC 2 cut(s) 429, 846
BsrFI RCCGGY 1 cut(s) 559
BsrI ACTGG 6 cut(s) 127, 1248, 1786, 1991, 2107, 2146
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 9 cut(s) 52, 338, 339, 502, 503, 1315, 1746, 1866, 2280
BssNI GRCGYC 2 cut(s) 303, 430
BssT1I CCWWGG 2 cut(s) 1866, 2280
Bst2UI CCWGG 4 cut(s) 110, 504, 599, 1317
Bst4CI ACNGT 4 cut(s) 376, 1747, 1814, 2050
Bst6I CTCTTC 3 cut(s) 681, 1361, 1553
BstACI GRCGYC 2 cut(s) 303, 430
BstAPI GCANNNNNTGC 1 cut(s) 169
BstBI TTCGAA 1 cut(s) 215
BstC8I GCNNGC 5 cut(s) 267, 392, 422, 1540, 1668
BstDEI CTNAG 8 cut(s) 842, 853, 888, 981, 1059, 1126, 2242, 2318
BstDSI CCRYGG 4 cut(s) 52, 1746, 1866, 2280
BstF5I GGATG 5 cut(s) 730, 1181, 1252, 1906, 2367
BstFNI CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 6 cut(s) 59, 75, 417, 432, 447, 1804
BstMAI GTCTC 3 cut(s) 202, 750, 990
BstMCI CGRYCG 1 cut(s) 257
BstMWI GCNNNNNNNGC 7 cut(s) 79, 169, 262, 400, 421, 2134, 2364
BstNI CCWGG 4 cut(s) 110, 504, 599, 1317
BstNSI RCATGY 2 cut(s) 1361, 1418
BstSCI CCNGG 7 cut(s) 108, 337, 338, 467, 502, 597, 1315
BstSFI CTRYAG 3 cut(s) 1641, 1650, 2215
BstUI CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
BstV1I GCAGC 4 cut(s) 411, 697, 2380, 2383
BstV2I GAAGAC 1 cut(s) 1137
BstX2I RGATCY 1 cut(s) 1597
BstYI RGATCY 1 cut(s) 1597
BstZI CGGCCG 1 cut(s) 254
Bsu36I CCTNAGG 2 cut(s) 842, 888
BsuI GTATCC 2 cut(s) 549, 1850
BsuRI GGCC 6 cut(s) 256, 337, 1198, 1635, 2279, 2303
BtgI CCRYGG 4 cut(s) 52, 1746, 1866, 2280
BtsCI GGATG 5 cut(s) 730, 1181, 1252, 1906, 2367
BtsI GCAGTG 1 cut(s) 1549
BtsIMutI CAGTG 3 cut(s) 1549, 2202, 2356
Cac8I GCNNGC 5 cut(s) 267, 392, 422, 1540, 1668
CaiI CAGNNNCTG 1 cut(s) 1613
CciI TCATGA 2 cut(s) 628, 1702
CfoI GCGC 6 cut(s) 59, 75, 417, 432, 447, 1804
Cfr10I RCCGGY 1 cut(s) 559
Cfr13I GGNCC 7 cut(s) 112, 336, 575, 601, 1196, 1989, 2277
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 338
CseI GACGC 2 cut(s) 320, 335
Csp6I GTAC 3 cut(s) 370, 1332, 1913
CspCI CAANNNNNGTGG 2 cut(s) 71, 106
CviQI GTAC 3 cut(s) 370, 1332, 1913
DdeI CTNAG 8 cut(s) 842, 853, 888, 981, 1059, 1126, 2242, 2318
DinI GGCGCC 1 cut(s) 431
DraIII CACNNNGTG 1 cut(s) 2178
EaeI YGGCCR 3 cut(s) 254, 1633, 2301
EagI CGGCCG 1 cut(s) 254
Eam1104I CTCTTC 3 cut(s) 681, 1361, 1553
EarI CTCTTC 3 cut(s) 681, 1361, 1553
EclXI CGGCCG 1 cut(s) 254
Eco130I CCWWGG 2 cut(s) 1866, 2280
Eco24I GRGCYC 2 cut(s) 11, 392
Eco47I GGWCC 4 cut(s) 112, 575, 601, 1989
Eco52I CGGCCG 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 1577
Eco81I CCTNAGG 2 cut(s) 842, 888
Eco88I CYCGRG 2 cut(s) 338, 462
EcoO109I RGGNCCY 1 cut(s) 1196
EcoRI GAATTC 3 cut(s) 824, 1680, 1732
EcoRII CCWGG 4 cut(s) 108, 502, 597, 1315
EcoT14I CCWWGG 2 cut(s) 1866, 2280
EcoT22I ATGCAT 1 cut(s) 730
EcoT38I GRGCYC 2 cut(s) 11, 392
EgeI GGCGCC 1 cut(s) 431
EheI GGCGCC 1 cut(s) 431
ErhI CCWWGG 2 cut(s) 1866, 2280
Esp3I CGTCTC 1 cut(s) 202
FaqI GGGAC 3 cut(s) 15, 115, 377
FauI CCCGC 1 cut(s) 399
FauNDI CATATG 1 cut(s) 1390
FbaI TGATCA 2 cut(s) 1699, 1705
FblI GTMKAC 2 cut(s) 1983, 2182
FokI GGATG 5 cut(s) 737, 1168, 1259, 1893, 2354
FriOI GRGCYC 2 cut(s) 11, 392
FspBI CTAG 5 cut(s) 734, 1341, 2016, 2111, 2390
GlaI GCGC 6 cut(s) 58, 74, 416, 431, 446, 1803
GsuI CTGGAG 4 cut(s) 425, 1635, 1803, 2150
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 6 cut(s) 256, 337, 1198, 1635, 2279, 2303
HapII CCGG 5 cut(s) 253, 339, 395, 468, 560
HgaI GACGC 2 cut(s) 320, 335
HhaI GCGC 6 cut(s) 59, 75, 417, 432, 447, 1804
Hin1I GRCGYC 2 cut(s) 303, 430
Hin6I GCGC 6 cut(s) 57, 73, 415, 430, 445, 1802
HinP1I GCGC 6 cut(s) 57, 73, 415, 430, 445, 1802
HincII GTYRAC 2 cut(s) 310, 1002
HindII GTYRAC 2 cut(s) 310, 1002
HpaII CCGG 5 cut(s) 253, 339, 395, 468, 560
HphI GGTGA 6 cut(s) 358, 1014, 1262, 1636, 1805, 2021
Hpy166II GTNNAC 7 cut(s) 310, 372, 459, 523, 1002, 1984, 2183
Hpy188III TCNNGA 7 cut(s) 629, 1363, 1403, 1614, 1677, 1703, 1937
Hpy8I GTNNAC 7 cut(s) 310, 372, 459, 523, 1002, 1984, 2183
Hpy99I CGWCG 6 cut(s) 80, 206, 209, 262, 305, 308
HpyAV CCTTC 9 cut(s) 7, 222, 466, 814, 1110, 1269, 1320, 1721, 2348
HpyCH4III ACNGT 4 cut(s) 376, 1747, 1814, 2050
HpyCH4IV ACGT 3 cut(s) 303, 538, 2022
HpyCH4V TGCA 7 cut(s) 728, 935, 1079, 1357, 1688, 2065, 2329
HpyF10VI GCNNNNNNNGC 7 cut(s) 79, 169, 262, 400, 421, 2134, 2364
HpyF3I CTNAG 8 cut(s) 842, 853, 888, 981, 1059, 1126, 2242, 2318
HpySE526I ACGT 3 cut(s) 303, 538, 2022
Hsp92I GRCGYC 2 cut(s) 303, 430
HspAI GCGC 6 cut(s) 57, 73, 415, 430, 445, 1802
KasI GGCGCC 1 cut(s) 429
Ksp22I TGATCA 2 cut(s) 1699, 1705
KspI CCGCGG 1 cut(s) 55
LmnI GCTCC 6 cut(s) 255, 406, 1503, 1535, 2134, 2404
Lsp1109I GCAGC 4 cut(s) 411, 697, 2380, 2383
LweI GCATC 4 cut(s) 474, 715, 2071, 2376
MaeI CTAG 5 cut(s) 734, 1341, 2016, 2111, 2390
MaeII ACGT 3 cut(s) 303, 538, 2022
MaeIII GTNAC 2 cut(s) 793, 1255
MflI RGATCY 1 cut(s) 1597
MhlI GDGCHC 2 cut(s) 11, 392
MluI ACGCGT 2 cut(s) 312, 327
Mly113I GGCGCC 1 cut(s) 430
MlyI GAGTC 3 cut(s) 454, 1127, 1794
MmeI TCCRAC 6 cut(s) 225, 733, 1209, 1261, 1625, 2395
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 2 cut(s) 824, 2358
MslI CAYNNNNRTG 2 cut(s) 633, 2285
MspA1I CMGCKG 2 cut(s) 54, 223
MspI CCGG 5 cut(s) 253, 339, 395, 468, 560
MspR9I CCNGG 7 cut(s) 110, 339, 340, 469, 504, 599, 1317
Mva1269I GAATGC 1 cut(s) 2358
MvaI CCWGG 4 cut(s) 110, 504, 599, 1317
MvnI CGCG 7 cut(s) 54, 59, 75, 314, 329, 417, 447
MwoI GCNNNNNNNGC 7 cut(s) 79, 169, 262, 400, 421, 2134, 2364
NarI GGCGCC 1 cut(s) 430
NciI CCSGG 3 cut(s) 339, 340, 469
NcoI CCATGG 2 cut(s) 1866, 2280
NdeI CATATG 1 cut(s) 1390
NlaIV GGNNCC 3 cut(s) 114, 431, 848
NmeAIII GCCGAG 1 cut(s) 1661
NmuCI GTSAC 1 cut(s) 793
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 2 cut(s) 1361, 1418
NspV TTCGAA 1 cut(s) 215
PaeR7I CTCGAG 1 cut(s) 462
PagI TCATGA 2 cut(s) 628, 1702
PasI CCCWGGG 1 cut(s) 503
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 2358
PdmI GAANNNNTTC 2 cut(s) 824, 2358
PfeI GAWTC 8 cut(s) 148, 154, 667, 804, 1433, 1619, 1862, 2309
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 3 cut(s) 454, 1127, 1793
PluTI GGCGCC 1 cut(s) 433
PpsI GAGTC 3 cut(s) 454, 1127, 1793
PsiI TTATAA 1 cut(s) 609
Psp6I CCWGG 4 cut(s) 108, 502, 597, 1315
PspGI CCWGG 4 cut(s) 108, 502, 597, 1315
PspN4I GGNNCC 3 cut(s) 114, 431, 848
PspPI GGNCC 7 cut(s) 112, 336, 575, 601, 1196, 1989, 2277
PspXI VCTCGAGB 1 cut(s) 462
PstNI CAGNNNCTG 1 cut(s) 1613
PsuI RGATCY 1 cut(s) 1597
RsaI GTAC 3 cut(s) 371, 1333, 1914
RsaNI GTAC 3 cut(s) 370, 1332, 1913
RseI CAYNNNNRTG 2 cut(s) 633, 2285
SacII CCGCGG 1 cut(s) 55
Sau96I GGNCC 7 cut(s) 112, 336, 575, 601, 1196, 1989, 2277
SchI GAGTC 3 cut(s) 454, 1127, 1794
ScrFI CCNGG 7 cut(s) 110, 339, 340, 469, 504, 599, 1317
SduI GDGCHC 2 cut(s) 11, 392
SfaNI GCATC 4 cut(s) 474, 715, 2071, 2376
SfcI CTRYAG 3 cut(s) 1641, 1650, 2215
SfoI GGCGCC 1 cut(s) 431
Sfr274I CTCGAG 1 cut(s) 462
Sfr303I CCGCGG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 4 cut(s) 112, 575, 601, 1989
SlaI CTCGAG 1 cut(s) 462
SmaI CCCGGG 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 633, 2285
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
SspDI GGCGCC 1 cut(s) 429
SspI AATATT 1 cut(s) 1858
SspMI CTAG 5 cut(s) 734, 1341, 2016, 2111, 2390
StyD4I CCNGG 7 cut(s) 108, 337, 338, 467, 502, 597, 1315
StyI CCWWGG 2 cut(s) 1866, 2280
TaaI ACNGT 4 cut(s) 376, 1747, 1814, 2050
TaiI ACGT 3 cut(s) 306, 541, 2025
TaqI TCGA 5 cut(s) 215, 237, 463, 670, 2382
TatI WGTACW 2 cut(s) 369, 1912
TauI GCSGC 9 cut(s) 54, 57, 223, 265, 420, 430, 450, 453, 1900
TfiI GAWTC 8 cut(s) 148, 154, 667, 804, 1433, 1619, 1862, 2309
TscAI CASTG 3 cut(s) 1549, 2209, 2356
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 4 cut(s) 424, 685, 2393, 2396
Tsp45I GTSAC 1 cut(s) 793
TspGWI ACGGA 5 cut(s) 242, 593, 1044, 1440, 2017
TspMI CCCGGG 1 cut(s) 338
TspRI CASTG 3 cut(s) 1549, 2209, 2356
VpaK11BI GGWCC 4 cut(s) 112, 575, 601, 1989
XapI RAATTY 5 cut(s) 824, 901, 1680, 1732, 1819
XceI RCATGY 2 cut(s) 1361, 1418
XhoI CTCGAG 1 cut(s) 462
XmaI CCCGGG 1 cut(s) 338
XmiI GTMKAC 2 cut(s) 1983, 2182
XmnI GAANNNNTTC 2 cut(s) 824, 2358
XspI CTAG 5 cut(s) 734, 1341, 2016, 2111, 2390
ZraI GACGTC 1 cut(s) 304
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.