Rh1DG379200

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000527
Physical Location & Seq
Reverse (-)
60110680 .. 60115317
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG379200.1

Sequence Viewer

Length: 2400 bp
ATGAAGAGCCCGAAGGAGGAGATCAGTTGTCCCCCATGTGAGTATTTCCGCCGCGGCGCGAAAGTCGAGGTGCGCGACGAGCAAGGTGCGTGGTTTCCTGCTCATGTCCTGGACCCAAATCCGTCCCCATTCTCGCTGCGGAATCGGAATCGGAAGCAGAAGTTGCTGGTGGTAGAGTATGAAACTCTCGTCTCCGACGACGACCCTTCGAAGCCGCTGACGGAGGAAATCGAGGTGGATATGCTCCGGCCTTCGCCGCCTGCGGAGGAGGAGGAGGGGTTTGAGGTAAACGACGTCGTTGACGCGCTTAATTTGGACGCGTGGTGGCCCGGGGTGGTGATGAGTGTTTTGGGGGACAAGTACACGGTGGGGTTTAAGAGCCCGCCGGATTTGCTGGAGCTTGTGCGCTGCGAGCTGCGGCGCCATTGGGATTGGCGCGGCGGCGAGTGGACTCGCGCCGGGAAGGAGATGATGATGCGATTGGATTTCAGCCCTGGGGAAGATGTTGAAGTGAACCTACACAAAGAACGTGTATGGTATGCTTGGATACCGGCAATTTATGTTGGTCCATTAGGGGGCAATTCTTTCCTGGTCCGTTATAAGGGTGCGAATAATGGTCATGAAAATGGTTTTGTTAAAGTTGTATCGGACTATCAGATTCGACCTCTTCCTCCACAGCAAGAGGAAAGAGCTTTTGAATTGTTGGGAATGGTGGATGCATACCTAGATATGTGTTGGTGGGTTGGAGACATTACAAAAGTTCTTACAGGGGAAAGGTATGTTGTCACATTAAGATTCACAAAGCAGGAGAAGGAATTCAGATCGTCAGACCTGAGGCACCACTCAGAATGGATTGGTAGCAAATGGGTGTCTGACCCTAAGGCACTTGAAAATTCTATTGTCAATGAAGAACGATTAAGATATGCAAGAAGCAATTACAGAAAATCCGAAGAAAAAACACCTTGTCATACTAAGTCTCAAATGAAGCAGTTGACTTGTCTTGTGAATAAATCACCTTACGGAAATACCATTAAGAAACTCAAATTATCTGAGCTTTCTAATGATGGTGCAACATTTTCAAGTTCAACTCCTTCAAAGAAGTTAAAAGGGGAACGCTCAGAAGACTCCTTATCTCTGTCAGCATTATTTGGCAGGGCAAAATCAATTAAAACATCCAACAGAAAAGGGCCTTTTGAAGATGGAGAGAAGTCTGAGCAACAACTTGTCGGGCAACTGGATGGTGAAGTAACCACTCCCGTAAAAAGAAGGGTTGGACAACGAAAATCACAAGCTGATAGCCCTTCCCCTGGTGTTAGACAGGGTACAAAGGCTAGACGACCAAAATTGCATGTCAAGAAGAGAAGTGGAATTGACAAAGCATATGAACAAAATCTTGAAGATGAGCATGTAATTAACGGAATTGAATCTTCTGGGATCGGTTCGGAGAATGAAGTAATGAGGGGATCAAAGGCTGGAAGCTCATGTCTGCTCCCTATGGAGGATAACCAACAACATAATGAAGATGGAGCAAGCAGTGGAATAAAACTGAAGAGAAAACTGCCTATGTTAGTTGTTGAACACTCAAAAGATCCAACAACAGGTTCTGGAGATTCGGTGAGAGATGGCCGAGCTGTAGCGTCTGTAGAGAAAGTTAAGCTGGCTGTGTCTGGAATTCCTGCAAAGAAACTTGATCATGATCAATCTTTATTCATAAAGGAAGGGAATTCTGTAAAAACCGTGGAGCGATCAATAAGTGGAAGAAAAATTGAAACTGGAGTCAAAGGTGATCTGGCGCAAGAAGACAGTCCAAATTTGCCTTTTGTGAAAAGTATCCCACTTTGGGAAAATATTGAATCCATGGAAGTATTCAAAAGATTTCCACAGAAGCCGCATTTTCATCCTGTACTGAAATCTAAAGAGGTGTTTCGTGAGGGATCGGCTTTCGGAAAAATGTTAGCCTTTAGCTCTTTGGTAGACCGGACCTCCAAGATACGGATTGGTGATCCTAGAAACGTCATTGATAGCAACTTGGAAGCGACAGTTGAATTGGAACAGCACGGCTTTGATGTTGATGCCATAAAGTGTCGTCTGACTGGGTTGCTAGAGCTGAAAGCCAAGCTGGAGGAGCTTCAAAACCAGTCCAAAGTAGTTGAAACCAAGATCACGGCGTGTAGACAAAACACAATCAAGGACACTGAAACTATCAGTAGAATTGACAAGGAGATCAAGGACTTAGAAGCAAACATCAAGGGCTTGAAGAAAAAACGGGCCATGGCTGTGTCAATGTGTTCGGCCAAAGATTCCGAGCTTAGCAAGTTGCAGTCAGAAGCAAAAGTTATCAGTGAAGGCATTCAGAGCATCCAACATGGTTTCGACATACTAGCTGCTGCTCCATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

799

Amino Acids

89.75

Weight (kDa)

8.27

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 19 - 87 1.4e-10 Agenet domain
Agenet PF05641 166 - 225 3e-06 Agenet domain
DUF724 PF05266 605 - 798 2.1e-53 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
AatII GACGTC 1 cut(s) 297
AccB1I GGYRCC 2 cut(s) 420, 837
AccI GTMKAC 2 cut(s) 1974, 2173
AccII CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
AclWI GGATC 5 cut(s) 1442, 1471, 1583, 1942, 1997
AcoI YGGCCR 2 cut(s) 1624, 2292
AcsI RAATTY 5 cut(s) 815, 892, 1671, 1723, 1810
AcuI CTGAAG 1 cut(s) 1568
AcyI GRCGYC 2 cut(s) 294, 421
AdeI CACNNNGTG 1 cut(s) 2169
AfaI GTAC 3 cut(s) 362, 1324, 1905
AfiI CCNNNNNNNGG 8 cut(s) 16, 575, 601, 1307, 1498, 1598, 1840, 1992
AflIII ACRYGT 2 cut(s) 318, 529
AjnI CCWGG 4 cut(s) 108, 493, 588, 1306
AjuI GAANNNNNNNTTGG 2 cut(s) 1585, 1617
Alw26I GTCTC 3 cut(s) 196, 741, 981
AlwI GGATC 5 cut(s) 1442, 1471, 1583, 1942, 1997
AlwNI CAGNNNCTG 1 cut(s) 1604
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 6 cut(s) 248, 326, 1187, 1624, 2268, 2292
ApeKI GCWGC 5 cut(s) 136, 408, 415, 2384, 2387
ApoI RAATTY 5 cut(s) 815, 892, 1671, 1723, 1810
Asp700I GAANNNNTTC 2 cut(s) 815, 2349
AspLEI GCGC 8 cut(s) 59, 75, 307, 408, 423, 438, 458, 1795
AspS9I GGNCC 7 cut(s) 112, 327, 566, 592, 1187, 1980, 2268
AsuC2I CCSGG 3 cut(s) 330, 331, 460
AsuHPI GGTGA 6 cut(s) 349, 1005, 1253, 1627, 1796, 2012
AsuII TTCGAA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 4 cut(s) 112, 566, 592, 1980
AxyI CCTNAGG 2 cut(s) 833, 879
BanI GGYRCC 2 cut(s) 420, 837
BanII GRGCYC 2 cut(s) 11, 383
BarI GAAGNNNNNNTAC 2 cut(s) 501, 533
BbsI GAAGAC 2 cut(s) 1128, 1806
BbvI GCAGC 5 cut(s) 123, 395, 402, 2371, 2374
BccI CCATC 5 cut(s) 1058, 1193, 1232, 1517, 1616
BceAI ACGGC 2 cut(s) 2074, 2181
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 4 cut(s) 110, 495, 590, 1308
BciVI GTATCC 2 cut(s) 540, 1841
BclI TGATCA 2 cut(s) 1690, 1696
BcnI CCSGG 3 cut(s) 330, 331, 460
BcoDI GTCTC 3 cut(s) 196, 741, 981
BfaI CTAG 5 cut(s) 725, 1332, 2007, 2102, 2381
BfmI CTRYAG 2 cut(s) 1632, 1641
BfoI RGCGCY 1 cut(s) 424
BfuI GTATCC 2 cut(s) 540, 1841
BlpI GCTNAGC 1 cut(s) 2309
Bme1390I CCNGG 7 cut(s) 110, 330, 331, 460, 495, 590, 1308
Bme18I GGWCC 4 cut(s) 112, 566, 592, 1980
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 7 cut(s) 112, 327, 566, 592, 1187, 1980, 2268
BmiI GGNNCC 3 cut(s) 114, 422, 839
BmrFI CCNGG 7 cut(s) 110, 330, 331, 460, 495, 590, 1308
BmrI ACTGGG 1 cut(s) 2103
BmsI GCATC 4 cut(s) 465, 706, 2062, 2367
BmuI ACTGGG 1 cut(s) 2103
BpiI GAAGAC 2 cut(s) 1128, 1806
BpmI CTGGAG 4 cut(s) 416, 1626, 1794, 2141
Bpu1102I GCTNAGC 1 cut(s) 2309
Bpu14I TTCGAA 1 cut(s) 209
BpuMI CCSGG 3 cut(s) 330, 331, 460
BsaBI GATNNNNATC 1 cut(s) 1695
BsaHI GRCGYC 2 cut(s) 294, 421
BsaJI CCNNGG 9 cut(s) 52, 329, 330, 493, 494, 1306, 1737, 1857, 2271
BsaWI WCCGGW 1 cut(s) 1977
BsaXI ACNNNNNCTCC 6 cut(s) 263, 293, 1599, 1629, 1967, 1997
Bsc4I CCNNNNNNNGG 8 cut(s) 16, 575, 601, 1307, 1498, 1598, 1840, 1992
Bse118I RCCGGY 1 cut(s) 550
Bse1I ACTGG 4 cut(s) 1239, 1777, 2098, 2137
Bse21I CCTNAGG 2 cut(s) 833, 879
Bse8I GATNNNNATC 1 cut(s) 1695
BseBI CCWGG 4 cut(s) 110, 495, 590, 1308
BseDI CCNNGG 9 cut(s) 52, 329, 330, 493, 494, 1306, 1737, 1857, 2271
BseGI GGATG 5 cut(s) 721, 1172, 1243, 1897, 2358
BseJI GATNNNNATC 1 cut(s) 1695
BseLI CCNNNNNNNGG 8 cut(s) 16, 575, 601, 1307, 1498, 1598, 1840, 1992
BseMII CTCAG 5 cut(s) 824, 858, 1041, 1131, 1203
BseNI ACTGG 4 cut(s) 1239, 1777, 2098, 2137
BseRI GAGGAG 5 cut(s) 32, 281, 284, 287, 2138
BseXI GCAGC 5 cut(s) 123, 395, 402, 2371, 2374
Bsh1236I CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
BshFI GGCC 6 cut(s) 250, 328, 1189, 1626, 2270, 2294
BshNI GGYRCC 2 cut(s) 420, 837
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 6 cut(s) 247, 330, 386, 459, 551, 1978
BslFI GGGAC 3 cut(s) 15, 109, 368
BslI CCNNNNNNNGG 8 cut(s) 16, 575, 601, 1307, 1498, 1598, 1840, 1992
BsmAI GTCTC 3 cut(s) 196, 741, 981
BsmBI CGTCTC 1 cut(s) 196
BsmFI GGGAC 3 cut(s) 15, 109, 368
BsmI GAATGC 1 cut(s) 2349
BsnI GGCC 6 cut(s) 250, 328, 1189, 1626, 2270, 2294
BsoBI CYCGRG 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 209
Bsp1286I GDGCHC 2 cut(s) 11, 383
Bsp1720I GCTNAGC 1 cut(s) 2309
Bsp19I CCATGG 2 cut(s) 1857, 2271
BspANI GGCC 6 cut(s) 250, 328, 1189, 1626, 2270, 2294
BspCNI CTCAG 5 cut(s) 825, 857, 1042, 1130, 1204
BspFNI CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
BspHI TCATGA 2 cut(s) 619, 1693
BspLI GGNNCC 3 cut(s) 114, 422, 839
BspPI GGATC 5 cut(s) 1442, 1471, 1583, 1942, 1997
BspT104I TTCGAA 1 cut(s) 209
BspT107I GGYRCC 2 cut(s) 420, 837
BsrFI RCCGGY 1 cut(s) 550
BsrI ACTGG 4 cut(s) 1239, 1777, 2098, 2137
BssAI RCCGGY 1 cut(s) 550
BssECI CCNNGG 9 cut(s) 52, 329, 330, 493, 494, 1306, 1737, 1857, 2271
BssNI GRCGYC 2 cut(s) 294, 421
BssT1I CCWWGG 2 cut(s) 1857, 2271
Bst2UI CCWGG 4 cut(s) 110, 495, 590, 1308
Bst4CI ACNGT 4 cut(s) 367, 1738, 1805, 2041
Bst6I CTCTTC 3 cut(s) 672, 1352, 1544
BstACI GRCGYC 2 cut(s) 294, 421
BstAPI GCANNNNNTGC 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 209
BstC8I GCNNGC 5 cut(s) 261, 383, 413, 1531, 1659
BstDEI CTNAG 9 cut(s) 833, 844, 879, 972, 1050, 1117, 1212, 2233, 2309
BstDSI CCRYGG 4 cut(s) 52, 1737, 1857, 2271
BstF5I GGATG 5 cut(s) 721, 1172, 1243, 1897, 2358
BstFNI CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 8 cut(s) 59, 75, 307, 408, 423, 438, 458, 1795
BstMAI GTCTC 3 cut(s) 196, 741, 981
BstMWI GCNNNNNNNGC 7 cut(s) 79, 163, 256, 391, 412, 2125, 2355
BstNI CCWGG 4 cut(s) 110, 495, 590, 1308
BstNSI RCATGY 2 cut(s) 1352, 1409
BstSCI CCNGG 7 cut(s) 108, 328, 329, 458, 493, 588, 1306
BstSFI CTRYAG 2 cut(s) 1632, 1641
BstUI CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
BstV1I GCAGC 5 cut(s) 123, 395, 402, 2371, 2374
BstV2I GAAGAC 2 cut(s) 1128, 1806
BstX2I RGATCY 1 cut(s) 1588
BstYI RGATCY 1 cut(s) 1588
Bsu36I CCTNAGG 2 cut(s) 833, 879
BsuI GTATCC 2 cut(s) 540, 1841
BsuRI GGCC 6 cut(s) 250, 328, 1189, 1626, 2270, 2294
BtgI CCRYGG 4 cut(s) 52, 1737, 1857, 2271
BtsCI GGATG 5 cut(s) 721, 1172, 1243, 1897, 2358
BtsI GCAGTG 1 cut(s) 1540
BtsIMutI CAGTG 3 cut(s) 1540, 2193, 2347
Cac8I GCNNGC 5 cut(s) 261, 383, 413, 1531, 1659
CaiI CAGNNNCTG 1 cut(s) 1604
CciI TCATGA 2 cut(s) 619, 1693
CfoI GCGC 8 cut(s) 59, 75, 307, 408, 423, 438, 458, 1795
Cfr10I RCCGGY 1 cut(s) 550
Cfr13I GGNCC 7 cut(s) 112, 327, 566, 592, 1187, 1980, 2268
Cfr42I CCGCGG 1 cut(s) 55
Cfr9I CCCGGG 1 cut(s) 329
CseI GACGC 3 cut(s) 311, 326, 1626
Csp6I GTAC 3 cut(s) 361, 1323, 1904
CspCI CAANNNNNGTGG 2 cut(s) 71, 106
CviQI GTAC 3 cut(s) 361, 1323, 1904
DdeI CTNAG 9 cut(s) 833, 844, 879, 972, 1050, 1117, 1212, 2233, 2309
DinI GGCGCC 1 cut(s) 422
DraIII CACNNNGTG 1 cut(s) 2169
EaeI YGGCCR 2 cut(s) 1624, 2292
Eam1104I CTCTTC 3 cut(s) 672, 1352, 1544
EarI CTCTTC 3 cut(s) 672, 1352, 1544
EciI GGCGGA 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 1857, 2271
Eco24I GRGCYC 2 cut(s) 11, 383
Eco47I GGWCC 4 cut(s) 112, 566, 592, 1980
Eco57I CTGAAG 1 cut(s) 1568
Eco81I CCTNAGG 2 cut(s) 833, 879
Eco88I CYCGRG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 1187
EcoRI GAATTC 3 cut(s) 815, 1671, 1723
EcoRII CCWGG 4 cut(s) 108, 493, 588, 1306
EcoT14I CCWWGG 2 cut(s) 1857, 2271
EcoT22I ATGCAT 1 cut(s) 721
EcoT38I GRGCYC 2 cut(s) 11, 383
EgeI GGCGCC 1 cut(s) 422
EheI GGCGCC 1 cut(s) 422
ErhI CCWWGG 2 cut(s) 1857, 2271
Esp3I CGTCTC 1 cut(s) 196
FaqI GGGAC 3 cut(s) 15, 109, 368
FauI CCCGC 1 cut(s) 390
FauNDI CATATG 1 cut(s) 1381
FbaI TGATCA 2 cut(s) 1690, 1696
FblI GTMKAC 2 cut(s) 1974, 2173
FokI GGATG 5 cut(s) 728, 1159, 1250, 1884, 2345
FriOI GRGCYC 2 cut(s) 11, 383
FspBI CTAG 5 cut(s) 725, 1332, 2007, 2102, 2381
GlaI GCGC 8 cut(s) 58, 74, 306, 407, 422, 437, 457, 1794
GsuI CTGGAG 4 cut(s) 416, 1626, 1794, 2141
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 6 cut(s) 250, 328, 1189, 1626, 2270, 2294
HapII CCGG 6 cut(s) 247, 330, 386, 459, 551, 1978
HgaI GACGC 3 cut(s) 311, 326, 1626
HhaI GCGC 8 cut(s) 59, 75, 307, 408, 423, 438, 458, 1795
Hin1I GRCGYC 2 cut(s) 294, 421
Hin6I GCGC 8 cut(s) 57, 73, 305, 406, 421, 436, 456, 1793
HinP1I GCGC 8 cut(s) 57, 73, 305, 406, 421, 436, 456, 1793
HincII GTYRAC 2 cut(s) 301, 993
HindII GTYRAC 2 cut(s) 301, 993
HpaII CCGG 6 cut(s) 247, 330, 386, 459, 551, 1978
HphI GGTGA 6 cut(s) 349, 1005, 1253, 1627, 1796, 2012
Hpy166II GTNNAC 8 cut(s) 289, 301, 363, 450, 514, 993, 1975, 2174
Hpy188III TCNNGA 7 cut(s) 620, 1354, 1394, 1605, 1668, 1694, 1928
Hpy8I GTNNAC 8 cut(s) 289, 301, 363, 450, 514, 993, 1975, 2174
Hpy99I CGWCG 5 cut(s) 80, 200, 203, 296, 299
HpyCH4III ACNGT 4 cut(s) 367, 1738, 1805, 2041
HpyCH4IV ACGT 3 cut(s) 294, 529, 2013
HpyCH4V TGCA 6 cut(s) 719, 926, 1070, 1348, 1679, 2320
HpyF10VI GCNNNNNNNGC 7 cut(s) 79, 163, 256, 391, 412, 2125, 2355
HpyF3I CTNAG 9 cut(s) 833, 844, 879, 972, 1050, 1117, 1212, 2233, 2309
HpySE526I ACGT 3 cut(s) 294, 529, 2013
Hsp92I GRCGYC 2 cut(s) 294, 421
HspAI GCGC 8 cut(s) 57, 73, 305, 406, 421, 436, 456, 1793
KasI GGCGCC 1 cut(s) 420
Ksp22I TGATCA 2 cut(s) 1690, 1696
KspI CCGCGG 1 cut(s) 55
LmnI GCTCC 7 cut(s) 249, 397, 1494, 1526, 1741, 2125, 2395
Lsp1109I GCAGC 5 cut(s) 123, 395, 402, 2371, 2374
LweI GCATC 4 cut(s) 465, 706, 2062, 2367
MaeI CTAG 5 cut(s) 725, 1332, 2007, 2102, 2381
MaeII ACGT 3 cut(s) 294, 529, 2013
MaeIII GTNAC 2 cut(s) 784, 1246
MflI RGATCY 1 cut(s) 1588
MhlI GDGCHC 2 cut(s) 11, 383
MluI ACGCGT 1 cut(s) 318
Mly113I GGCGCC 1 cut(s) 421
MlyI GAGTC 3 cut(s) 445, 1118, 1785
MmeI TCCRAC 6 cut(s) 219, 724, 1200, 1252, 1616, 2386
Mph1103I ATGCAT 1 cut(s) 721
MroXI GAANNNNTTC 2 cut(s) 815, 2349
MslI CAYNNNNRTG 2 cut(s) 624, 2276
MspA1I CMGCKG 2 cut(s) 54, 217
MspI CCGG 6 cut(s) 247, 330, 386, 459, 551, 1978
MspR9I CCNGG 7 cut(s) 110, 330, 331, 460, 495, 590, 1308
Mva1269I GAATGC 1 cut(s) 2349
MvaI CCWGG 4 cut(s) 110, 495, 590, 1308
MvnI CGCG 7 cut(s) 54, 59, 75, 305, 320, 438, 456
MwoI GCNNNNNNNGC 7 cut(s) 79, 163, 256, 391, 412, 2125, 2355
NarI GGCGCC 1 cut(s) 421
NciI CCSGG 3 cut(s) 330, 331, 460
NcoI CCATGG 2 cut(s) 1857, 2271
NdeI CATATG 1 cut(s) 1381
NlaIV GGNNCC 3 cut(s) 114, 422, 839
NmeAIII GCCGAG 1 cut(s) 1652
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 1 cut(s) 721
NspI RCATGY 2 cut(s) 1352, 1409
NspV TTCGAA 1 cut(s) 209
PagI TCATGA 2 cut(s) 619, 1693
PasI CCCWGGG 1 cut(s) 494
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 195
PctI GAATGC 1 cut(s) 2349
PdmI GAANNNNTTC 2 cut(s) 815, 2349
PfeI GAWTC 8 cut(s) 142, 148, 658, 795, 1424, 1610, 1853, 2300
PfoI TCCNGGA 1 cut(s) 108
PleI GAGTC 3 cut(s) 445, 1118, 1784
PluTI GGCGCC 1 cut(s) 424
PpsI GAGTC 3 cut(s) 445, 1118, 1784
PsiI TTATAA 1 cut(s) 600
Psp6I CCWGG 4 cut(s) 108, 493, 588, 1306
PspGI CCWGG 4 cut(s) 108, 493, 588, 1306
PspN4I GGNNCC 3 cut(s) 114, 422, 839
PspPI GGNCC 7 cut(s) 112, 327, 566, 592, 1187, 1980, 2268
PstNI CAGNNNCTG 1 cut(s) 1604
PsuI RGATCY 1 cut(s) 1588
RsaI GTAC 3 cut(s) 362, 1324, 1905
RsaNI GTAC 3 cut(s) 361, 1323, 1904
RseI CAYNNNNRTG 2 cut(s) 624, 2276
SacII CCGCGG 1 cut(s) 55
Sau96I GGNCC 7 cut(s) 112, 327, 566, 592, 1187, 1980, 2268
SchI GAGTC 3 cut(s) 445, 1118, 1785
ScrFI CCNGG 7 cut(s) 110, 330, 331, 460, 495, 590, 1308
SduI GDGCHC 2 cut(s) 11, 383
SfaNI GCATC 4 cut(s) 465, 706, 2062, 2367
SfcI CTRYAG 2 cut(s) 1632, 1641
SfoI GGCGCC 1 cut(s) 422
Sfr303I CCGCGG 1 cut(s) 55
SfuI TTCGAA 1 cut(s) 209
SgrBI CCGCGG 1 cut(s) 55
SinI GGWCC 4 cut(s) 112, 566, 592, 1980
SmaI CCCGGG 1 cut(s) 331
SmiMI CAYNNNNRTG 2 cut(s) 624, 2276
SspDI GGCGCC 1 cut(s) 420
SspI AATATT 1 cut(s) 1849
SspMI CTAG 5 cut(s) 725, 1332, 2007, 2102, 2381
StyD4I CCNGG 7 cut(s) 108, 328, 329, 458, 493, 588, 1306
StyI CCWWGG 2 cut(s) 1857, 2271
TaaI ACNGT 4 cut(s) 367, 1738, 1805, 2041
TaiI ACGT 3 cut(s) 297, 532, 2016
TaqI TCGA 5 cut(s) 66, 209, 231, 661, 2373
TatI WGTACW 2 cut(s) 360, 1903
TauI GCSGC 8 cut(s) 54, 57, 217, 259, 421, 441, 444, 1891
TfiI GAWTC 8 cut(s) 142, 148, 658, 795, 1424, 1610, 1853, 2300
TscAI CASTG 3 cut(s) 1540, 2200, 2347
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 5 cut(s) 136, 408, 415, 2384, 2387
Tsp45I GTSAC 1 cut(s) 784
TspGWI ACGGA 6 cut(s) 111, 236, 584, 1035, 1431, 2008
TspMI CCCGGG 1 cut(s) 329
TspRI CASTG 3 cut(s) 1540, 2200, 2347
VpaK11BI GGWCC 4 cut(s) 112, 566, 592, 1980
XapI RAATTY 5 cut(s) 815, 892, 1671, 1723, 1810
XceI RCATGY 2 cut(s) 1352, 1409
XmaI CCCGGG 1 cut(s) 329
XmiI GTMKAC 2 cut(s) 1974, 2173
XmnI GAANNNNTTC 2 cut(s) 815, 2349
XspI CTAG 5 cut(s) 725, 1332, 2007, 2102, 2381
ZraI GACGTC 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.