Rroxscaffold_4G00284480

agenet domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000527
Physical Location & Seq
Forward (+)
6070985 .. 6077064
6080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00284480.1

Sequence Viewer

Length: 2769 bp
ATGGCGAAACCGAGCAGCAATACTCGGACTTGGTTCTGGAGCCGAGGCTCGAAAGTGGAGGTGCGCACAGAAGAAGAAGGCTTCCACGGCGCGTGGTTCCCGGCGACGATCCTGGAGCCGCCGTCGAATCCTTCTTCGGTTCTCAGCAGGAAACGAAAGAGCTTCAACTCGAACAAGGTCTTGGTCCAGTACAACACCTTCGTCACGGAGAAGGACCCAACCAAGCCGCTGACGGAGTACACCAACGTGATGTTCCTCCGGCCGTCGCCGCCGGTGGAGGCTCCCGGGAAGATATTCGAGGCCGGCGACTACGTGGACGCGTTTCACAACGACGCGTGGTGGCCGGGAGTGGTGATGACTGTGGAAGGAGGGGACAGGTACAGGGTGGGGTTTAGGGACCCGCCGGAGTTGCTGGTTCTTGGCCGGAGTGAGTTGAGGAGTCACTTGGATTGGGTCAACGAGATTTGGTCCAGACCATACAAGAAGAGCCTAAAGAACAATGATAAAGATGGGATTGCTACAGTGGCTGTATCTGACCATCAGATCCGACCTCGCCCTCCACGGCACAACGAAACAGGTTTTTGCTTGCTGGACATGGTGGATGCGTTCCATGATAACAGTTGGTGGGTTGGAAGTATTACAAATGTTCTTCCAGACAAAAAGTACATTGTCACTTTTAAACTTACAGATGAACAGAAGGAATTCAGTCACTCAGACTTAAGGCTCCATATGGAATGGATTGACAAAAAATGGGAGTCTGACTTTTCGAGATTTGGGTCTGCGATGGATGATATAGAACAAACACAGCATACATTCACAAATTCCAGAAACTCTGTGGTAGCTACACCACTTAAAAGGTCACCTGCTATGAAGGATAGCACGGGAGAAGAAAATCCTTATGCTAGCTCACAGAAGAATCAGATGAAGCAGCTGGGTCCTTGCATTGATAAATCAGCTTATAGGAAAACCACAAATGAACTGAAACTACTGGACCAACCTAATGATGATGCAACATTTTTAAGTCCAACTCCTTCCAAGAAGTTAAAACGACATCCAGAAGAATCTGTTTCTATTGATGAAGTATATGTGAGGAGAAAACCAATTAATATATCCAATGAAAAAGGCCCTGTTGGAGATGGTGTGAAGACTGGGTCGCTGCTTGGAGAACCGGGTAGACAAAGAAAATCACAATTTGATGGGAGCAGTCCACCACTGAAAATTCATGTTGCAGTCAAGAAGGGAAGTAGAATGGACAAGGCTGATGAAGAATGTATAGGCGATGAGCATGTAATGGACAAGACTGAATCTTCTGGTATTCAGTCAGACTCTGAAGTAACGAGAAGATCACTTGCTGGAAATTATAGTCTCCTGCATTTGAAGGAGATTAATCAACATGATGAAGACGGAGTGAGCAATCAGACAAAAGTGAAGGGTAAGCTGCCTGATATACTTGTTGAACACTTGAGAGATTCTGAAGATTCAACAAGAAATGGGACAACAGAAGCATCTGGAATGGAAGTTCAGGTGACTGTGCCTGGATTGTCTGCAAAGCCTGCTGCTCATGATCAGCCAGTATCATTGTGTACAGATGAAATGCCTTCTGAATACACCATTGAGTGCTTAGTTAATCCGGTTGATGAAATGCATTCTCAATGCACTCTTAATCAGGATGACAAAATGTTTCATGAAGGCACTATGGAGTGTGCAGGTAACCCTGCTGCAACTACCAACCAACAAATTCCAGTTGCTGAGGCTGGACTACCCGGAAAGACACCTACTCATGATCAGCCTCTACCATTGTGCATATACAAGATGCATTCTGAAAAGACTATGGAGGGCTCAAGTTTTGAAGATCCAACAGAAGATGGAAAAGCTCAAGCTACTGCAATGGAAGTAGAGGTGGCTGGGACTGAAATATCCTCAGAGACACCTCTTCATGATCAACCTTTATTATTGTGTGACATGGACTCTGAAAACACTTTGGAGGGTTACCCTTCTGGGACTGCAAAAACATCCCTTCATGATCAATTTCAGGCATCAAGCATAGCTGAAATGTATTCTGAACATACTACTGAGGGCTCATGCAAACGTGCTGGAACAGCAAAGCAACAATATGAGGTTACAGGGAGACAAGGTGAAACCGGAATTACCAAGGACATTGAGCAAGAAGACACTCCACATCTGCCTTTTGTGAAAAAATCTCCATTTTGGAAAATGTTGGAATCCATGGATGCATTCAAAAGGTTTCCGCAACTGCCACATTTTAAGCCCTTACTGAAGGCTAAAATGTTATGCCGTGAAGGACTAGCCCTTGGAGGTATGCATATCTTTGCCGCTGTGGTCGAGTACACTTCGAAGCTGCAGGTTGATGATCCTAGTGACTCGATCAACGACATTTTGGAAGCTCTAGTTGAACTGGAACGGCTGGGATTTGATGTCAAGGTACTCCAGGACCGACTAAATGAATTGCAAGACATGAAACTTAGGTTTGCAGAGCTTAAGAACAAGTCAGACGGAGTCAAAATCCTGATCACGAATTGTACGCATGAAAGAAGCAAAGGCTCTGAAGTTATCAGTGGGATGGATGCGGAGATCAATGACTTGGAGGAAAAGATCAGAGGGATACAGGCAAAAAGGGCAAAGGTGGTGTCAGAGAGGGCGGCGAAAGATTCTGAAATCATTATGTTGGAATCAGAAGCAGATGCTATCAATGAAGGCATTCAGAGCATCGAGCATGATTTCCAAAATCTAGCAGCAGCTGTATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

922

Amino Acids

103.15

Weight (kDa)

5.29

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 16 - 91 5.2e-14 Agenet domain
DUF724 PF05266 728 - 920 6e-51 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 871
Acc16I TGCGCA 1 cut(s) 65
Acc36I ACCTGC 3 cut(s) 871, 1697, 2353
AccI GTMKAC 1 cut(s) 1174
AccII CGCG 3 cut(s) 92, 320, 335
AciI CCGC 8 cut(s) 119, 227, 269, 401, 2249, 2334, 2588, 2660
AclWI GGATC 4 cut(s) 103, 538, 1847, 2366
AcoI YGGCCR 3 cut(s) 260, 341, 421
AcsI RAATTY 4 cut(s) 701, 820, 1218, 1737
AcuI CTGAAG 4 cut(s) 1350, 1494, 2297, 2586
AfaI GTAC 8 cut(s) 191, 239, 380, 665, 1585, 2348, 2445, 2542
AflII CTTAAG 2 cut(s) 718, 2498
AflIII ACRYGT 2 cut(s) 318, 333
AgsI TTSAA 7 cut(s) 166, 1378, 1457, 1482, 1850, 2239, 2414
AjnI CCWGG 3 cut(s) 111, 1534, 2448
AjuI GAANNNNNNNTTGG 4 cut(s) 164, 196, 236, 268
AleI CACNNNNGTG 1 cut(s) 245
AloI GAACNNNNNNTCC 2 cut(s) 1503, 1535
Alw26I GTCTC 3 cut(s) 1370, 1919, 2122
AlwI GGATC 4 cut(s) 103, 538, 1847, 2366
AlwNI CAGNNNCTG 4 cut(s) 527, 1328, 1748, 2759
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 5 cut(s) 260, 300, 341, 421, 1123
ApeKI GCWGC 9 cut(s) 15, 928, 1156, 1438, 1556, 1718, 2359, 2753, 2756
ApoI RAATTY 4 cut(s) 701, 820, 1218, 1737
ArsI GACNNNNNNTTYG 2 cut(s) 2633, 2665
AseI ATTAAT 2 cut(s) 1104, 1386
Asp700I GAANNNNTTC 3 cut(s) 293, 701, 2718
AspLEI GCGC 2 cut(s) 66, 92
AspS9I GGNCC 8 cut(s) 184, 214, 397, 468, 935, 991, 1124, 2452
AsuC2I CCSGG 6 cut(s) 101, 285, 286, 345, 1170, 1764
AsuHPI GGTGA 4 cut(s) 364, 852, 1537, 2147
AsuII TTCGAA 1 cut(s) 2354
AsuNHI GCTAGC 1 cut(s) 902
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 7 cut(s) 184, 214, 397, 468, 935, 991, 2452
BanII GRGCYC 2 cut(s) 1841, 2081
BarI GAAGNNNNNNTAC 2 cut(s) 182, 214
BbsI GAAGAC 3 cut(s) 1151, 1407, 2175
BbvCI CCTCAGC 1 cut(s) 1749
BbvI GCAGC 8 cut(s) 27, 940, 1143, 1425, 1543, 1705, 2346, 2765
BccI CCATC 7 cut(s) 503, 546, 778, 1130, 1190, 1859, 2575
BceAI ACGGC 6 cut(s) 103, 106, 247, 578, 2280, 2438
BciT130I CCWGG 3 cut(s) 113, 1536, 2450
BciVI GTATCC 1 cut(s) 2616
BclI TGATCA 5 cut(s) 1564, 1783, 1939, 2023, 2529
BcnI CCSGG 6 cut(s) 101, 285, 286, 345, 1170, 1764
BcoDI GTCTC 3 cut(s) 1370, 1919, 2122
BfaI CTAG 5 cut(s) 903, 2306, 2376, 2408, 2750
BfmI CTRYAG 2 cut(s) 519, 2360
BfrI CTTAAG 2 cut(s) 718, 2498
BfuAI ACCTGC 3 cut(s) 871, 1697, 2353
BfuI GTATCC 1 cut(s) 2616
Bme1390I CCNGG 9 cut(s) 101, 113, 285, 286, 345, 1170, 1536, 1764, 2450
Bme18I GGWCC 7 cut(s) 184, 214, 397, 468, 935, 991, 2452
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 8 cut(s) 184, 214, 397, 468, 935, 991, 1124, 2452
BmiI GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 725, 936
BmrFI CCNGG 9 cut(s) 101, 113, 285, 286, 345, 1170, 1536, 1764, 2450
BmrI ACTGGG 1 cut(s) 1158
BmsI GCATC 9 cut(s) 592, 997, 1514, 1803, 2045, 2221, 2575, 2692, 2736
BmtI GCTAGC 1 cut(s) 906
BmuI ACTGGG 1 cut(s) 1158
BpiI GAAGAC 3 cut(s) 1151, 1407, 2175
BpmI CTGGAG 3 cut(s) 58, 134, 2432
Bpu10I CCTNAGC 1 cut(s) 1749
Bpu14I TTCGAA 1 cut(s) 2354
BpuEI CTTGAG 3 cut(s) 1483, 1825, 1860
BpuMI CCSGG 6 cut(s) 101, 285, 286, 345, 1170, 1764
BsaAI YACGTR 1 cut(s) 313
BsaJI CCNNGG 7 cut(s) 43, 85, 284, 560, 2151, 2226, 2311
BsaWI WCCGGW 2 cut(s) 1630, 2141
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 1125, 1155, 2119, 2149
Bse118I RCCGGY 2 cut(s) 271, 302
Bse1I ACTGG 6 cut(s) 187, 993, 1153, 1571, 1742, 2421
Bse3DI GCAATG 1 cut(s) 1893
BseBI CCWGG 3 cut(s) 113, 1536, 2450
BseDI CCNNGG 7 cut(s) 43, 85, 284, 560, 2151, 2226, 2311
BseGI GGATG 8 cut(s) 607, 793, 1051, 1675, 2012, 2236, 2586, 2590
BseMI GCAATG 1 cut(s) 1893
BseMII CTCAG 5 cut(s) 157, 726, 1740, 1935, 2064
BseNI ACTGG 6 cut(s) 187, 993, 1153, 1571, 1742, 2421
BseRI GAGGAG 2 cut(s) 451, 1105
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 8 cut(s) 27, 940, 1143, 1425, 1543, 1705, 2346, 2765
BseYI CCCAGC 3 cut(s) 931, 1904, 2425
BsgI GTGCAG 1 cut(s) 1725
Bsh1236I CGCG 3 cut(s) 92, 320, 335
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 5 cut(s) 262, 302, 343, 423, 1125
BsiEI CGRYCG 1 cut(s) 263
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 5 cut(s) 386, 410, 1507, 1921, 2014
BsmAI GTCTC 3 cut(s) 1370, 1919, 2122
BsmFI GGGAC 5 cut(s) 386, 410, 1507, 1921, 2014
BsmI GAATGC 4 cut(s) 1645, 1816, 2234, 2718
BsnI GGCC 5 cut(s) 262, 302, 343, 423, 1125
BsoBI CYCGRG 1 cut(s) 284
Bsp119I TTCGAA 1 cut(s) 2354
Bsp1286I GDGCHC 2 cut(s) 1841, 2081
Bsp1407I TGTACA 1 cut(s) 1583
Bsp19I CCATGG 1 cut(s) 2226
BspACI CCGC 8 cut(s) 119, 227, 269, 401, 2249, 2334, 2588, 2660
BspANI GGCC 5 cut(s) 262, 302, 343, 423, 1125
BspCNI CTCAG 5 cut(s) 156, 725, 1741, 1934, 2065
BspFNI CGCG 3 cut(s) 92, 320, 335
BspHI TCATGA 5 cut(s) 1561, 1684, 1780, 1936, 2020
BspLI GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 725, 936
BspMAI CTGCAG 1 cut(s) 2364
BspMI ACCTGC 3 cut(s) 871, 1697, 2353
BspOI GCTAGC 1 cut(s) 906
BspPI GGATC 4 cut(s) 103, 538, 1847, 2366
BspQI GCTCTTC 1 cut(s) 479
BspT104I TTCGAA 1 cut(s) 2354
BspTI CTTAAG 2 cut(s) 718, 2498
BsrDI GCAATG 1 cut(s) 1893
BsrFI RCCGGY 2 cut(s) 271, 302
BsrGI TGTACA 1 cut(s) 1583
BsrI ACTGG 6 cut(s) 187, 993, 1153, 1571, 1742, 2421
BssAI RCCGGY 2 cut(s) 271, 302
BssECI CCNNGG 7 cut(s) 43, 85, 284, 560, 2151, 2226, 2311
BssT1I CCWWGG 3 cut(s) 2151, 2226, 2311
Bst2UI CCWGG 3 cut(s) 113, 1536, 2450
Bst4CI ACNGT 4 cut(s) 361, 523, 620, 1531
Bst6I CTCTTC 2 cut(s) 479, 1938
BstAFI CTTAAG 2 cut(s) 718, 2498
BstAPI GCANNNNNTGC 1 cut(s) 1553
BstAUI TGTACA 1 cut(s) 1583
BstBAI YACGTR 1 cut(s) 313
BstBI TTCGAA 1 cut(s) 2354
BstC8I GCNNGC 4 cut(s) 304, 587, 904, 1554
BstDEI CTNAG 7 cut(s) 143, 712, 1621, 1749, 1921, 2073, 2483
BstDSI CCRYGG 3 cut(s) 85, 560, 2226
BstEII GGTNACC 3 cut(s) 858, 1709, 1988
BstF5I GGATG 8 cut(s) 607, 793, 1051, 1675, 2012, 2236, 2586, 2590
BstFNI CGCG 3 cut(s) 92, 320, 335
BstHHI GCGC 2 cut(s) 66, 92
BstMAI GTCTC 3 cut(s) 1370, 1919, 2122
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 8 cut(s) 87, 268, 409, 524, 1553, 2099, 2636, 2724
BstNI CCWGG 3 cut(s) 113, 1536, 2450
BstNSI RCATGY 1 cut(s) 1289
BstPI GGTNACC 3 cut(s) 858, 1709, 1988
BstSCI CCNGG 9 cut(s) 99, 111, 283, 284, 343, 1168, 1534, 1762, 2448
BstSFI CTRYAG 2 cut(s) 519, 2360
BstUI CGCG 3 cut(s) 92, 320, 335
BstV1I GCAGC 8 cut(s) 27, 940, 1143, 1425, 1543, 1705, 2346, 2765
BstV2I GAAGAC 3 cut(s) 1151, 1407, 2175
BstX2I RGATCY 2 cut(s) 543, 1852
BstYI RGATCY 2 cut(s) 543, 1852
BstZI CGGCCG 1 cut(s) 260
BsuI GTATCC 1 cut(s) 2616
BsuRI GGCC 5 cut(s) 262, 302, 343, 423, 1125
BtgI CCRYGG 3 cut(s) 85, 560, 2226
BtgZI GCGATG 2 cut(s) 797, 1293
BtsCI GGATG 8 cut(s) 607, 793, 1051, 1675, 2012, 2236, 2586, 2590
BtsIMutI CAGTG 3 cut(s) 528, 1211, 2581
BveI ACCTGC 3 cut(s) 871, 1697, 2353
Cac8I GCNNGC 4 cut(s) 304, 587, 904, 1554
CaiI CAGNNNCTG 4 cut(s) 527, 1328, 1748, 2759
CciI TCATGA 5 cut(s) 1561, 1684, 1780, 1936, 2020
CfoI GCGC 2 cut(s) 66, 92
Cfr10I RCCGGY 2 cut(s) 271, 302
Cfr13I GGNCC 8 cut(s) 184, 214, 397, 468, 935, 991, 1124, 2452
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 2 cut(s) 326, 341
Csp6I GTAC 8 cut(s) 190, 238, 379, 664, 1584, 2347, 2444, 2541
CviQI GTAC 8 cut(s) 190, 238, 379, 664, 1584, 2347, 2444, 2541
DdeI CTNAG 7 cut(s) 143, 712, 1621, 1749, 1921, 2073, 2483
DraI TTTAAA 1 cut(s) 679
EaeI YGGCCR 3 cut(s) 260, 341, 421
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 2 cut(s) 479, 1938
EarI CTCTTC 2 cut(s) 479, 1938
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 3 cut(s) 2151, 2226, 2311
Eco24I GRGCYC 2 cut(s) 1841, 2081
Eco47I GGWCC 7 cut(s) 184, 214, 397, 468, 935, 991, 2452
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 4 cut(s) 1350, 1494, 2297, 2586
Eco88I CYCGRG 1 cut(s) 284
Eco91I GGTNACC 3 cut(s) 858, 1709, 1988
EcoO109I RGGNCCY 4 cut(s) 214, 397, 935, 1124
EcoO65I GGTNACC 3 cut(s) 858, 1709, 1988
EcoRI GAATTC 1 cut(s) 701
EcoRII CCWGG 3 cut(s) 111, 1534, 2448
EcoT14I CCWWGG 3 cut(s) 2151, 2226, 2311
EcoT22I ATGCAT 4 cut(s) 1647, 1818, 2236, 2325
EcoT38I GRGCYC 2 cut(s) 1841, 2081
ErhI CCWWGG 3 cut(s) 2151, 2226, 2311
FaqI GGGAC 5 cut(s) 386, 410, 1507, 1921, 2014
FauI CCCGC 1 cut(s) 408
FauNDI CATATG 1 cut(s) 729
FbaI TGATCA 5 cut(s) 1564, 1783, 1939, 2023, 2529
FblI GTMKAC 1 cut(s) 1174
FokI GGATG 8 cut(s) 614, 800, 1038, 1682, 1999, 2243, 2593, 2597
FriOI GRGCYC 2 cut(s) 1841, 2081
FspAI RTGCGCAY 1 cut(s) 65
FspBI CTAG 5 cut(s) 903, 2306, 2376, 2408, 2750
FspI TGCGCA 1 cut(s) 65
GlaI GCGC 2 cut(s) 65, 91
GsaI CCCAGC 3 cut(s) 935, 1908, 2429
GsuI CTGGAG 3 cut(s) 58, 134, 2432
HaeIII GGCC 5 cut(s) 262, 302, 343, 423, 1125
HgaI GACGC 2 cut(s) 326, 341
HhaI GCGC 2 cut(s) 66, 92
Hin6I GCGC 2 cut(s) 64, 90
HinP1I GCGC 2 cut(s) 64, 90
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HphI GGTGA 4 cut(s) 364, 852, 1537, 2147
Hpy166II GTNNAC 7 cut(s) 240, 316, 457, 1175, 1208, 1584, 2349
Hpy8I GTNNAC 7 cut(s) 240, 316, 457, 1175, 1208, 1584, 2349
Hpy99I CGWCG 4 cut(s) 109, 127, 268, 335
HpyCH4III ACNGT 4 cut(s) 361, 523, 620, 1531
HpyCH4IV ACGT 3 cut(s) 246, 312, 2089
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 268, 409, 524, 1553, 2099, 2636, 2724
HpyF3I CTNAG 7 cut(s) 143, 712, 1621, 1749, 1921, 2073, 2483
HpySE526I ACGT 3 cut(s) 246, 312, 2089
HspAI GCGC 2 cut(s) 64, 90
KflI GGGWCCC 1 cut(s) 397
KroI GCCGGC 1 cut(s) 302
KroNI GCCGGC 1 cut(s) 304
Ksp22I TGATCA 5 cut(s) 1564, 1783, 1939, 2023, 2529
LguI GCTCTTC 1 cut(s) 479
LmnI GCTCC 5 cut(s) 39, 115, 286, 729, 1200
Lsp1109I GCAGC 8 cut(s) 27, 940, 1143, 1425, 1543, 1705, 2346, 2765
LweI GCATC 9 cut(s) 592, 997, 1514, 1803, 2045, 2221, 2575, 2692, 2736
MaeI CTAG 5 cut(s) 903, 2306, 2376, 2408, 2750
MaeII ACGT 3 cut(s) 246, 312, 2089
MflI RGATCY 2 cut(s) 543, 1852
MhlI GDGCHC 2 cut(s) 1841, 2081
MluI ACGCGT 2 cut(s) 318, 333
MlyI GAGTC 6 cut(s) 448, 764, 1319, 1961, 2375, 2526
MmeI TCCRAC 7 cut(s) 571, 610, 1049, 1111, 1880, 2199, 2667
Mph1103I ATGCAT 4 cut(s) 1647, 1818, 2236, 2325
MroNI GCCGGC 1 cut(s) 302
MroXI GAANNNNTTC 3 cut(s) 293, 701, 2718
MslI CAYNNNNRTG 1 cut(s) 245
MspA1I CMGCKG 4 cut(s) 229, 931, 2336, 2759
MspCI CTTAAG 2 cut(s) 718, 2498
MspR9I CCNGG 9 cut(s) 101, 113, 285, 286, 345, 1170, 1536, 1764, 2450
Mva1269I GAATGC 4 cut(s) 1645, 1816, 2234, 2718
MvaI CCWGG 3 cut(s) 113, 1536, 2450
MvnI CGCG 3 cut(s) 92, 320, 335
MwoI GCNNNNNNNGC 8 cut(s) 87, 268, 409, 524, 1553, 2099, 2636, 2724
NaeI GCCGGC 1 cut(s) 304
NciI CCSGG 6 cut(s) 101, 285, 286, 345, 1170, 1764
NcoI CCATGG 1 cut(s) 2226
NdeI CATATG 1 cut(s) 729
NgoMIV GCCGGC 1 cut(s) 302
NheI GCTAGC 1 cut(s) 902
NlaIV GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 725, 936
NmeAIII GCCGAG 1 cut(s) 68
NmuCI GTSAC 8 cut(s) 202, 440, 670, 707, 858, 1525, 1958, 2378
NsbI TGCGCA 1 cut(s) 65
NsiI ATGCAT 4 cut(s) 1647, 1818, 2236, 2325
NspI RCATGY 1 cut(s) 1289
NspV TTCGAA 1 cut(s) 2354
OliI CACNNNNGTG 1 cut(s) 245
PagI TCATGA 5 cut(s) 1561, 1684, 1780, 1936, 2020
PaqCI CACCTGC 1 cut(s) 871
PciSI GCTCTTC 1 cut(s) 479
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 4 cut(s) 1645, 1816, 2234, 2718
PdiI GCCGGC 1 cut(s) 304
PdmI GAANNNNTTC 3 cut(s) 293, 701, 2718
PfeI GAWTC 9 cut(s) 127, 916, 1061, 1304, 1469, 1478, 2222, 2669, 2690
PflFI GACNNNGTC 2 cut(s) 1150, 2516
PfoI TCCNGGA 2 cut(s) 111, 2448
PleI GAGTC 6 cut(s) 447, 763, 1319, 1961, 2375, 2525
PpsI GAGTC 6 cut(s) 447, 763, 1319, 1961, 2375, 2525
Ppu21I YACGTR 1 cut(s) 313
PpuMI RGGWCCY 3 cut(s) 214, 397, 935
PshBI ATTAAT 2 cut(s) 1104, 1386
Psp5II RGGWCCY 3 cut(s) 214, 397, 935
Psp6I CCWGG 3 cut(s) 111, 1534, 2448
PspEI GGTNACC 3 cut(s) 858, 1709, 1988
PspFI CCCAGC 3 cut(s) 931, 1904, 2425
PspGI CCWGG 3 cut(s) 111, 1534, 2448
PspN4I GGNNCC 9 cut(s) 41, 98, 117, 216, 282, 398, 399, 725, 936
PspPI GGNCC 8 cut(s) 184, 214, 397, 468, 935, 991, 1124, 2452
PspPPI RGGWCCY 3 cut(s) 214, 397, 935
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PstI CTGCAG 1 cut(s) 2364
PstNI CAGNNNCTG 4 cut(s) 527, 1328, 1748, 2759
PsuI RGATCY 2 cut(s) 543, 1852
PsyI GACNNNGTC 2 cut(s) 1150, 2516
PvuII CAGCTG 2 cut(s) 931, 2759
RsaI GTAC 8 cut(s) 191, 239, 380, 665, 1585, 2348, 2445, 2542
RsaNI GTAC 8 cut(s) 190, 238, 379, 664, 1584, 2347, 2444, 2541
RseI CAYNNNNRTG 1 cut(s) 245
SapI GCTCTTC 1 cut(s) 479
Sau96I GGNCC 8 cut(s) 184, 214, 397, 468, 935, 991, 1124, 2452
SchI GAGTC 6 cut(s) 448, 764, 1319, 1961, 2375, 2526
ScrFI CCNGG 9 cut(s) 101, 113, 285, 286, 345, 1170, 1536, 1764, 2450
SduI GDGCHC 2 cut(s) 1841, 2081
SfaNI GCATC 9 cut(s) 592, 997, 1514, 1803, 2045, 2221, 2575, 2692, 2736
SfcI CTRYAG 2 cut(s) 519, 2360
SfuI TTCGAA 1 cut(s) 2354
SgrAI CRCCGGYG 1 cut(s) 271
SinI GGWCC 7 cut(s) 184, 214, 397, 468, 935, 991, 2452
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 245
SmlI CTYRAG 5 cut(s) 718, 1462, 1840, 1875, 2498
SmoI CTYRAG 5 cut(s) 718, 1462, 1840, 1875, 2498
SsiI CCGC 8 cut(s) 119, 227, 269, 401, 2249, 2334, 2588, 2660
SspMI CTAG 5 cut(s) 903, 2306, 2376, 2408, 2750
StyD4I CCNGG 9 cut(s) 99, 111, 283, 284, 343, 1168, 1534, 1762, 2448
StyI CCWWGG 3 cut(s) 2151, 2226, 2311
TaaI ACNGT 4 cut(s) 361, 523, 620, 1531
TaiI ACGT 3 cut(s) 249, 315, 2092
TaqI TCGA 9 cut(s) 50, 125, 170, 297, 767, 2343, 2354, 2384, 2730
TaqII GACCGA 1 cut(s) 2469
TatI WGTACW 5 cut(s) 189, 237, 663, 1583, 2346
TauI GCSGC 5 cut(s) 121, 229, 271, 2336, 2663
TfiI GAWTC 9 cut(s) 127, 916, 1061, 1304, 1469, 1478, 2222, 2669, 2690
TscAI CASTG 3 cut(s) 528, 1218, 2581
TseFI GTSAC 8 cut(s) 202, 440, 670, 707, 858, 1525, 1958, 2378
TseI GCWGC 9 cut(s) 15, 928, 1156, 1438, 1556, 1718, 2359, 2753, 2756
Tsp45I GTSAC 8 cut(s) 202, 440, 670, 707, 858, 1525, 1958, 2378
TspGWI ACGGA 4 cut(s) 221, 248, 1419, 2529
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 3 cut(s) 528, 1218, 2581
Tth111I GACNNNGTC 2 cut(s) 1150, 2516
Vha464I CTTAAG 2 cut(s) 718, 2498
VpaK11BI GGWCC 7 cut(s) 184, 214, 397, 468, 935, 991, 2452
VspI ATTAAT 2 cut(s) 1104, 1386
XapI RAATTY 4 cut(s) 701, 820, 1218, 1737
XceI RCATGY 1 cut(s) 1289
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 1174
XmnI GAANNNNTTC 3 cut(s) 293, 701, 2718
XspI CTAG 5 cut(s) 903, 2306, 2376, 2408, 2750
Zsp2I ATGCAT 4 cut(s) 1647, 1818, 2236, 2325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.