RLG00000026853

Agenet domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000527
Physical Location & Seq
Forward (+)
6530895 .. 6535630
4736 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026853

Sequence Viewer

Length: 2382 bp
ATGGAGGAGCAGGAGAGCAATGGTGGCCATAACCCTGTTGGGTGTTTCACCCAAGGCGCCAACGTCGAAGTTTGCAGCAAAGAAAAAGGGTCTGAAGGTGCATGGTTCCCTGCAACAATCTTAGAGCCACCAGCAAACTCCTCAGCCTCAAAAAGAAAGACCTTTACTTCCAACAAGGTTTTGATTCAGTACAAGACTCTTGTCTCCGACAAGGACCCCACCAAGCCACTTGTCGAGCACACCACCGTGGGGCTTCTCCGGCCGCCGCCTCCGGCTGACAATGCTGAGGAGGAGAGGTTTGAGGAGAACGACGTCGTTGATGCTTTTCACCTTGAAGGTTGGTGGACTGGGTTGGTGATGAGTGTTGCGGGGGAGAAGTATGGTGTGGGGTTTAAGAAACCGCCGGATTATCTGGAGCTAGAGCGCGGTAAGTTGAGGTCACATTGGGATTGGGACCATGGGGTTTGGACCAGAGCCCCAAGACAGGTGTTGAGTGGATCAAATTACAAGCCATGGACACCAGTTGAAGTGAATCTGGAAAAAGATCATCTATGGTCTTCATGGTTACCTGCATTTTTTATTGGTCAAGTAGGGGTTGACTCTTTTTTGGTGCAATACAAATATAAGACCCTAGAAAATGATGATAAAAATGGTCTTGTTAAAGTAGTTGTAACTGATCACCAGATTCGACCTCGCCCTCCACAGCAAGAGGAAAAAGATTTTCACTTGTCGCAAATGGTGGATGCGTTCTGTGATATGGGTTGGTGGGTAGGGGACATTACAGAAGTTCTTACACACAAGAGGTACATTGTCACTTTCAGATTCATAAAGGAGGAGAAAGAGTTTTGTCATTCAGAGTTAAGGTGCCACTTGGAATGGATTGGTAAACAATGGGTGACTAGCGGTCCGGAATTTCATTCTACTAAGGATCGTGGAGTACAACCAAGACATGCACATGAAATTTCCAGTAATTCTGTAGGCAAAACACCTTGTCATACCAAGTTTAGGATGAAGCAATTGAGTCCTTGCATGGATGAGTTACCTTGTGAAAGAACCAAAGACAAACTGAAAATATCTCAGCATCCTAGTGATGATTCAACATTTTTAAGTCCCACTCCCTGCAAGAAGTTAAAAGGGGAAGGTTCAGAAGACACCTTGTCTCTTGCACAATCCTATCAAAGGAAAACCCCAGCTAAAACATCCAAGAGGAAAGGCCTATTTACTGATGAAGAGACGAATGAGCAACAGCAGTTCGAACAACTGGATAGTCTAGCAATCAATTTTGTAAAAAGGGGGAGACAACCAAACTCACAATTTGTAAAATGTGGAAGGAAGGGTATACCCCGAAAATTGCATGTCAGCAATGGCAGTAAAAGTGTAAGAGGTGACCAAGAATACATTGAAGATGAAAATGTAATGACCAAGATTGAATCTTTTGGTATCTCGTCAGAGCCTGAAGCAATTGGGGGCTTGCATGCTGGAAATTCATGTTTGCTTCCTGTGAAGGAGATTAAACATGGTAATGAAGATAAAGTGAGCAGTGGAATAAAAGGGAAGAAAAAACAGGCTGAGTTACTTGTTCAACACCCAAAAGATCCAGCAGCAGGTTGTATGAAAGATATACTTGAAAAGCCTGCAAATCCAAGGGGGAACCAAAACCTGGCAACTTACCAAAGAAAGAAAAGGTTCAAACTGACAGGTGTAAAGCATGACGGAACTGGAGTTTTTACTGATGTTGAAGTGTCCAAGACTAATGTTGTTGAACATATATGTAACCCTCCTGGGACTGTCAACCAACAAGAAAAAAGTCCAAATCTGCCTTTTGTGAAGAATTCTCCACTATGGGAACATCTTGAATCCATGGAAGTATTCAAAAACTTTCCGCAGAAGCCGCATTTTCATTTTGCGGTGAACTTGAATCCTGTATTTCGTGAAGGAGCAGCCATCGGCAGTATGTTAGCCTTTGTTTCTTTGGTTGAGAAGATTTCCAATTTACGAGTTGATGATCCTAGAGAATTAATCGAAAGCTATTTGAAAGGACTAGTTGAAATGGAATTGATGGGATTTGATGTCAAAGCACTAAGGCATCATCTGACTGAATTGCTAGACATGAAAATAAAGTTGGGACAGCTTCAGAAACAATCAAAAGAAGTTAAACTTAGGATCACAGGGAGTACTCATGACACAACCACCTATGATGAAACAATCACTGGGATTGACAAGAAGATTAAGGACTTGCAAGAAAAACGGATGATGGCAGTGTCAATGAAAGAGGTCAAAGTTTCTGAAATTAGCAGGTTGCAAGCAGAAGCAACAGCTATCAATGAAGACATCCAGAGTATCAGGTGTGATTTTGGAAACCTAGTGGCTGCTGAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Agenet DUF724

Protein Analysis

794

Amino Acids

89.8

Weight (kDa)

7.16

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Agenet PF05641 19 - 91 4.2e-13 Agenet domain
Agenet PF05641 173 - 234 3.7e-07 Agenet domain
DUF724 PF05266 606 - 791 3.5e-54 Protein of unknown function (DUF724)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000527)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03300 AT1G11420 AT1G26540 AT2G47230 AT2G47230 AT3G62300 AT3G62300 AT5G23770 AT5G23770 AT5G23780 AT5G23780 AT5G23800 AT5G23800
fragaria_vesca FvH4_7g26470 FvH4_7g26480 FvH4_7g26480 FvH4_7g26480
malus_domestica MD01G1173400.v1.1 MD01G1179100.v1.1 MD07G1101600.v1.1 MD07G1240300.v1.1
prunus_persica Prupe.2G061500_v2.0.a1 Prupe.2G061500_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G061600_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269000_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.2G269100_v2.0.a1 Prupe.3G124100_v2.0.a1
pyrus_communis pycom01g18520 pycom07g21670 pycom07g27230
rosa_chinensis RchiOBHm_Chr1g0373061 RchiOBHm_Chr1g0373071 RchiOBHm_Chr1g0373081
rosa_laevigata RLG00000001149 RLG00000016652 RLG00000026851 RLG00000026852 RLG00000026853
rosa_multiflora Rmu_co8353763.1_g000001 Rmu_sc0033012.1_g000001 Rmu_sc0033013.1_g000001 Rmu_sc0034594.1_g000001 Rmu_sc0035726.1_g000001 Rmu_ssc0000425.1_g000006 Rmu_ssc0000425.1_g000007
rosa_roxburghii Rroxscaffold_2G00111710 Rroxscaffold_3G00228370 Rroxscaffold_4G00284480 Rroxscaffold_4G00284490 Rroxscaffold_4G00284500 Rroxscaffold_5G00355260
rosa_rugosa Rorug01G0375800 Rorug01G0375900 Rorug01G0376000
rosa_samantha Rh1AG384100 Rh1AG384200 Rh1AG384300 Rh1BG348100 Rh1BG348300 Rh1BG348400 Rh1CG361500 Rh1CG361700 Rh1CG361800 Rh1DG379000 Rh1DG379200 Rh1DG379300 Rh7AG386700
rosa_wichuraiana Rw1G033870 Rw1G033880 Rw1G033890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 3 cut(s) 577, 1595, 2286
AccB1I GGYRCC 2 cut(s) 56, 864
AccB7I CCANNNNNTGG 1 cut(s) 1660
AccI GTMKAC 1 cut(s) 1339
AccII CGCG 1 cut(s) 426
AccIII TCCGGA 1 cut(s) 907
AciI CCGC 9 cut(s) 263, 266, 368, 401, 426, 903, 1883, 1892, 1907
AclWI GGATC 5 cut(s) 505, 936, 1589, 2000, 2171
AcoI YGGCCR 2 cut(s) 25, 260
AcsI RAATTY 4 cut(s) 911, 960, 1483, 1831
AcuI CTGAAG 3 cut(s) 114, 1476, 2117
AcyI GRCGYC 2 cut(s) 57, 312
AfaI GTAC 4 cut(s) 191, 806, 939, 2176
AfiI CCNNNNNNNGG 6 cut(s) 249, 484, 1005, 1179, 1604, 1660
AhdI GACNNNNNGTC 2 cut(s) 903, 1156
AhlI ACTAGT 1 cut(s) 2041
AjnI CCWGG 2 cut(s) 1659, 1780
AjuI GAANNNNNNNTTGG 2 cut(s) 2105, 2137
AleI CACNNNNGTG 1 cut(s) 245
AluBI AGCT 5 cut(s) 418, 1193, 2028, 2131, 2318
AluI AGCT 5 cut(s) 418, 1193, 2028, 2131, 2318
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 4 cut(s) 208, 1164, 1226, 1291
AlwI GGATC 5 cut(s) 505, 936, 1589, 2000, 2171
AlwNI CAGNNNCTG 1 cut(s) 1454
Aor13HI TCCGGA 1 cut(s) 907
AoxI GGCC 3 cut(s) 25, 260, 1213
ApeKI GCWGC 4 cut(s) 75, 1601, 1940, 2369
ApoI RAATTY 4 cut(s) 911, 960, 1483, 1831
AseI ATTAAT 1 cut(s) 2018
Asp700I GAANNNNTTC 1 cut(s) 1685
AspLEI GCGC 2 cut(s) 59, 426
AspS9I GGNCC 4 cut(s) 214, 454, 468, 905
AsuHPI GGTGA 7 cut(s) 40, 320, 367, 671, 907, 1397, 1921
AsuII TTCGAA 1 cut(s) 1254
AvaII GGWCC 4 cut(s) 214, 454, 468, 905
BalI TGGCCA 1 cut(s) 27
BanI GGYRCC 2 cut(s) 56, 864
BanII GRGCYC 1 cut(s) 478
BbsI GAAGAC 3 cut(s) 549, 1155, 2334
Bbv12I GWGCWC 1 cut(s) 240
BbvCI CCTCAGC 2 cut(s) 142, 285
BbvI GCAGC 4 cut(s) 87, 1613, 1952, 2356
BccI CCATC 3 cut(s) 1952, 2053, 2248
BciT130I CCWGG 2 cut(s) 1661, 1782
BclI TGATCA 1 cut(s) 676
BcoDI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BcuI ACTAGT 1 cut(s) 2041
BfaI CTAG 9 cut(s) 419, 632, 900, 1086, 1271, 2010, 2042, 2105, 2363
BfmI CTRYAG 1 cut(s) 975
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 3 cut(s) 577, 1595, 2286
BisI GCNGC 7 cut(s) 76, 263, 266, 1602, 1892, 1941, 2370
BlsI GCNGC 7 cut(s) 77, 264, 267, 1603, 1893, 1942, 2371
BmcAI AGTACT 1 cut(s) 2176
Bme1390I CCNGG 2 cut(s) 1661, 1782
Bme18I GGWCC 4 cut(s) 214, 454, 468, 905
BmeRI GACNNNNNGTC 2 cut(s) 903, 1156
BmgT120I GGNCC 4 cut(s) 214, 454, 468, 905
BmiI GGNNCC 6 cut(s) 58, 107, 216, 455, 866, 1652
BmrFI CCNGG 2 cut(s) 1661, 1782
BmrI ACTGGG 2 cut(s) 357, 2220
BmsI GCATC 4 cut(s) 310, 733, 1090, 2095
BmuI ACTGGG 2 cut(s) 357, 2220
BoxI GACNNNNGTC 1 cut(s) 200
BpiI GAAGAC 3 cut(s) 549, 1155, 2334
BpmI CTGGAG 2 cut(s) 434, 1740
Bpu10I CCTNAGC 2 cut(s) 142, 285
Bpu14I TTCGAA 1 cut(s) 1254
BsaHI GRCGYC 2 cut(s) 57, 312
BsaJI CCNNGG 7 cut(s) 52, 246, 457, 512, 1643, 1781, 1860
BsaWI WCCGGW 1 cut(s) 907
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 827, 857
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 484, 1005, 1179, 1604, 1660
Bse1I ACTGG 6 cut(s) 352, 521, 966, 1266, 1723, 2215
Bse3DI GCAATG 2 cut(s) 25, 1369
BseAI TCCGGA 1 cut(s) 907
BseBI CCWGG 2 cut(s) 1661, 1782
BseDI CCNNGG 7 cut(s) 52, 246, 457, 512, 1643, 1781, 1860
BseGI GGATG 7 cut(s) 748, 1014, 1039, 1081, 1199, 2256, 2331
BseLI CCNNNNNNNGG 6 cut(s) 249, 484, 1005, 1179, 1604, 1660
BseMI GCAATG 2 cut(s) 25, 1369
BseMII CTCAG 4 cut(s) 156, 276, 1091, 1560
BseNI ACTGG 6 cut(s) 352, 521, 966, 1266, 1723, 2215
BseRI GAGGAG 6 cut(s) 20, 130, 302, 305, 317, 848
BseX3I CGGCCG 1 cut(s) 260
BseXI GCAGC 4 cut(s) 87, 1613, 1952, 2356
BseYI CCCAGC 1 cut(s) 1189
Bsh1236I CGCG 1 cut(s) 426
Bsh1285I CGRYCG 1 cut(s) 263
BshFI GGCC 3 cut(s) 27, 262, 1215
BshNI GGYRCC 2 cut(s) 56, 864
BsiEI CGRYCG 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 4 cut(s) 259, 272, 404, 908
BslFI GGGAC 5 cut(s) 467, 788, 1095, 1798, 2139
BslI CCNNNNNNNGG 6 cut(s) 249, 484, 1005, 1179, 1604, 1660
BsmAI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BsmBI CGTCTC 1 cut(s) 1226
BsmFI GGGAC 5 cut(s) 467, 788, 1095, 1798, 2139
BsnI GGCC 3 cut(s) 27, 262, 1215
Bsp119I TTCGAA 1 cut(s) 1254
Bsp1286I GDGCHC 2 cut(s) 240, 478
Bsp13I TCCGGA 1 cut(s) 907
Bsp143I GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
Bsp19I CCATGG 3 cut(s) 457, 512, 1860
BspACI CCGC 9 cut(s) 263, 266, 368, 401, 426, 903, 1883, 1892, 1907
BspANI GGCC 3 cut(s) 27, 262, 1215
BspCNI CTCAG 4 cut(s) 155, 277, 1090, 1561
BspEI TCCGGA 1 cut(s) 907
BspFNI CGCG 1 cut(s) 426
BspHI TCATGA 1 cut(s) 2179
BspLI GGNNCC 6 cut(s) 58, 107, 216, 455, 866, 1652
BspMI ACCTGC 3 cut(s) 577, 1595, 2286
BspPI GGATC 5 cut(s) 505, 936, 1589, 2000, 2171
BspT104I TTCGAA 1 cut(s) 1254
BspT107I GGYRCC 2 cut(s) 56, 864
BsrDI GCAATG 2 cut(s) 25, 1369
BsrI ACTGG 6 cut(s) 352, 521, 966, 1266, 1723, 2215
BssECI CCNNGG 7 cut(s) 52, 246, 457, 512, 1643, 1781, 1860
BssMI GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
BssNAI GTATAC 1 cut(s) 1340
BssNI GRCGYC 2 cut(s) 57, 312
BssT1I CCWWGG 5 cut(s) 52, 457, 512, 1643, 1860
Bst1107I GTATAC 1 cut(s) 1340
Bst2UI CCWGG 2 cut(s) 1661, 1782
Bst4CI ACNGT 2 cut(s) 247, 1789
Bst6I CTCTTC 1 cut(s) 1224
BstACI GRCGYC 2 cut(s) 57, 312
BstBI TTCGAA 1 cut(s) 1254
BstC8I GCNNGC 4 cut(s) 1472, 1476, 1635, 2304
BstDEI CTNAG 8 cut(s) 121, 142, 285, 924, 1077, 1569, 2081, 2159
BstDSI CCRYGG 4 cut(s) 246, 457, 512, 1860
BstEII GGTNACC 2 cut(s) 564, 1385
BstENI CCTNNNNNAGG 1 cut(s) 1177
BstF5I GGATG 7 cut(s) 748, 1014, 1039, 1081, 1199, 2256, 2331
BstFNI CGCG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 426
BstKTI GATC 7 cut(s) 500, 547, 679, 931, 1597, 2008, 2166
BstMAI GTCTC 4 cut(s) 208, 1164, 1226, 1291
BstMBI GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
BstMCI CGRYCG 1 cut(s) 263
BstMWI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1891
BstNI CCWGG 2 cut(s) 1661, 1782
BstNSI RCATGY 3 cut(s) 953, 1358, 1478
BstPAI GACNNNNGTC 1 cut(s) 200
BstPI GGTNACC 2 cut(s) 564, 1385
BstSCI CCNGG 2 cut(s) 1659, 1780
BstSFI CTRYAG 1 cut(s) 975
BstUI CGCG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 87, 1613, 1952, 2356
BstV2I GAAGAC 3 cut(s) 549, 1155, 2334
BstX2I RGATCY 1 cut(s) 1594
BstYI RGATCY 1 cut(s) 1594
BstZ17I GTATAC 1 cut(s) 1340
BstZI CGGCCG 1 cut(s) 260
BsuRI GGCC 3 cut(s) 27, 262, 1215
BtgI CCRYGG 4 cut(s) 246, 457, 512, 1860
BtsCI GGATG 7 cut(s) 748, 1014, 1039, 1081, 1199, 2256, 2331
BtsI GCAGTG 2 cut(s) 1546, 2265
BtsIMutI CAGTG 3 cut(s) 1546, 2208, 2265
BveI ACCTGC 3 cut(s) 577, 1595, 2286
Cac8I GCNNGC 4 cut(s) 1472, 1476, 1635, 2304
CaiI CAGNNNCTG 1 cut(s) 1454
CciI TCATGA 1 cut(s) 2179
CfoI GCGC 2 cut(s) 59, 426
Cfr13I GGNCC 4 cut(s) 214, 454, 468, 905
CpoI CGGWCCG 1 cut(s) 905
Csp6I GTAC 4 cut(s) 190, 805, 938, 2175
CspI CGGWCCG 1 cut(s) 905
CviQI GTAC 4 cut(s) 190, 805, 938, 2175
DdeI CTNAG 8 cut(s) 121, 142, 285, 924, 1077, 1569, 2081, 2159
DinI GGCGCC 1 cut(s) 58
DpnI GATC 7 cut(s) 499, 546, 678, 930, 1596, 2007, 2165
DpnII GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
DriI GACNNNNNGTC 2 cut(s) 903, 1156
EaeI YGGCCR 2 cut(s) 25, 260
EagI CGGCCG 1 cut(s) 260
Eam1104I CTCTTC 1 cut(s) 1224
Eam1105I GACNNNNNGTC 2 cut(s) 903, 1156
EarI CTCTTC 1 cut(s) 1224
EclXI CGGCCG 1 cut(s) 260
Eco130I CCWWGG 5 cut(s) 52, 457, 512, 1643, 1860
Eco147I AGGCCT 1 cut(s) 1215
Eco24I GRGCYC 1 cut(s) 478
Eco47I GGWCC 4 cut(s) 214, 454, 468, 905
Eco52I CGGCCG 1 cut(s) 260
Eco57I CTGAAG 3 cut(s) 114, 1476, 2117
Eco91I GGTNACC 2 cut(s) 564, 1385
EcoNI CCTNNNNNAGG 1 cut(s) 1177
EcoO109I RGGNCCY 1 cut(s) 214
EcoO65I GGTNACC 2 cut(s) 564, 1385
EcoRI GAATTC 1 cut(s) 1831
EcoRII CCWGG 2 cut(s) 1659, 1780
EcoT14I CCWWGG 5 cut(s) 52, 457, 512, 1643, 1860
EcoT38I GRGCYC 1 cut(s) 478
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 5 cut(s) 52, 457, 512, 1643, 1860
Esp3I CGTCTC 1 cut(s) 1226
FalI AAGNNNNNCTT 4 cut(s) 1608, 1640, 2142, 2174
FaqI GGGAC 5 cut(s) 467, 788, 1095, 1798, 2139
FauI CCCGC 1 cut(s) 361
FbaI TGATCA 1 cut(s) 676
FblI GTMKAC 1 cut(s) 1339
Fnu4HI GCNGC 7 cut(s) 76, 263, 266, 1602, 1892, 1941, 2370
FokI GGATG 7 cut(s) 755, 1021, 1046, 1068, 1186, 2263, 2318
FriOI GRGCYC 1 cut(s) 478
Fsp4HI GCNGC 7 cut(s) 76, 263, 266, 1602, 1892, 1941, 2370
FspBI CTAG 9 cut(s) 419, 632, 900, 1086, 1271, 2010, 2042, 2105, 2363
GlaI GCGC 2 cut(s) 58, 425
GluI GCNGC 7 cut(s) 76, 263, 266, 1602, 1892, 1941, 2370
GsaI CCCAGC 1 cut(s) 1193
GsuI CTGGAG 2 cut(s) 434, 1740
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 27, 262, 1215
HapII CCGG 4 cut(s) 259, 272, 404, 908
HhaI GCGC 2 cut(s) 59, 426
Hin1I GRCGYC 2 cut(s) 57, 312
Hin6I GCGC 2 cut(s) 57, 424
HinP1I GCGC 2 cut(s) 57, 424
HincII GTYRAC 2 cut(s) 598, 1792
HindII GTYRAC 2 cut(s) 598, 1792
HpaII CCGG 4 cut(s) 259, 272, 404, 908
HphI GGTGA 7 cut(s) 40, 320, 367, 671, 907, 1397, 1921
Hpy166II GTNNAC 6 cut(s) 345, 598, 887, 1340, 1792, 1912
Hpy188I TCNGA 9 cut(s) 94, 208, 821, 856, 1147, 1450, 2094, 2136, 2287
Hpy188III TCNNGA 7 cut(s) 413, 536, 908, 1853, 1931, 2180, 2335
Hpy8I GTNNAC 6 cut(s) 345, 598, 887, 1340, 1792, 1912
Hpy99I CGWCG 3 cut(s) 68, 314, 317
HpyAV CCTTC 7 cut(s) 89, 329, 1133, 1323, 1327, 1498, 1928
HpyCH4III ACNGT 2 cut(s) 247, 1789
HpyCH4IV ACGT 2 cut(s) 63, 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1891
HpyF3I CTNAG 8 cut(s) 121, 142, 285, 924, 1077, 1569, 2081, 2159
HpySE526I ACGT 2 cut(s) 63, 312
Hsp92I GRCGYC 2 cut(s) 57, 312
HspAI GCGC 2 cut(s) 57, 424
KasI GGCGCC 1 cut(s) 56
Kpn2I TCCGGA 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 676
Kzo9I GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
LmnI GCTCC 3 cut(s) 7, 415, 1937
Lsp1109I GCAGC 4 cut(s) 87, 1613, 1952, 2356
LweI GCATC 4 cut(s) 310, 733, 1090, 2095
MaeI CTAG 9 cut(s) 419, 632, 900, 1086, 1271, 2010, 2042, 2105, 2363
MaeII ACGT 2 cut(s) 63, 312
MaeIII GTNAC 9 cut(s) 438, 564, 670, 811, 895, 1038, 1385, 1572, 1772
MalI GATC 7 cut(s) 499, 546, 678, 930, 1596, 2007, 2165
MboI GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
MfeI CAATTG 2 cut(s) 1016, 1461
MflI RGATCY 1 cut(s) 1594
MhlI GDGCHC 2 cut(s) 240, 478
MlsI TGGCCA 1 cut(s) 27
MluNI TGGCCA 1 cut(s) 27
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 3 cut(s) 190, 593, 1030
MmeI TCCRAC 2 cut(s) 195, 231
Mox20I TGGCCA 1 cut(s) 27
MroI TCCGGA 1 cut(s) 907
MroXI GAANNNNTTC 1 cut(s) 1685
MscI TGGCCA 1 cut(s) 27
MseI TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2018, 2154, 2229
MslI CAYNNNNRTG 4 cut(s) 245, 954, 1086, 1521
Msp20I TGGCCA 1 cut(s) 27
MspI CCGG 4 cut(s) 259, 272, 404, 908
MspR9I CCNGG 2 cut(s) 1661, 1782
MunI CAATTG 2 cut(s) 1016, 1461
MvaI CCWGG 2 cut(s) 1661, 1782
MvnI CGCG 1 cut(s) 426
MwoI GCNNNNNNNGC 4 cut(s) 24, 259, 281, 1891
NarI GGCGCC 1 cut(s) 57
NcoI CCATGG 3 cut(s) 457, 512, 1860
NdeII GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
NlaIV GGNNCC 6 cut(s) 58, 107, 216, 455, 866, 1652
NmuCI GTSAC 4 cut(s) 438, 811, 895, 1385
NspI RCATGY 3 cut(s) 953, 1358, 1478
NspV TTCGAA 1 cut(s) 1254
OliI CACNNNNGTG 1 cut(s) 245
PaeI GCATGC 1 cut(s) 1478
PagI TCATGA 1 cut(s) 2179
PceI AGGCCT 1 cut(s) 1215
PdmI GAANNNNTTC 1 cut(s) 1685
PfeI GAWTC 8 cut(s) 184, 532, 685, 822, 1094, 1430, 1856, 1918
PflMI CCANNNNNTGG 1 cut(s) 1660
PkrI GCNGC 7 cut(s) 77, 264, 267, 1603, 1893, 1942, 2371
PleI GAGTC 3 cut(s) 190, 593, 1029
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 3 cut(s) 190, 593, 1029
PpuMI RGGWCCY 1 cut(s) 214
PshAI GACNNNNGTC 1 cut(s) 200
PshBI ATTAAT 1 cut(s) 2018
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 1659, 1780
PspEI GGTNACC 2 cut(s) 564, 1385
PspFI CCCAGC 1 cut(s) 1189
PspGI CCWGG 2 cut(s) 1659, 1780
PspN4I GGNNCC 6 cut(s) 58, 107, 216, 455, 866, 1652
PspPI GGNCC 4 cut(s) 214, 454, 468, 905
PspPPI RGGWCCY 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 1454
PsuI RGATCY 1 cut(s) 1594
RsaI GTAC 4 cut(s) 191, 806, 939, 2176
RsaNI GTAC 4 cut(s) 190, 805, 938, 2175
RseI CAYNNNNRTG 4 cut(s) 245, 954, 1086, 1521
Rsr2I CGGWCCG 1 cut(s) 905
RsrII CGGWCCG 1 cut(s) 905
SaqAI TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2018, 2154, 2229
SatI GCNGC 7 cut(s) 76, 263, 266, 1602, 1892, 1941, 2370
Sau3AI GATC 7 cut(s) 497, 544, 676, 928, 1594, 2005, 2163
Sau96I GGNCC 4 cut(s) 214, 454, 468, 905
ScaI AGTACT 1 cut(s) 2176
SchI GAGTC 3 cut(s) 190, 593, 1030
ScrFI CCNGG 2 cut(s) 1661, 1782
SduI GDGCHC 2 cut(s) 240, 478
SfaNI GCATC 4 cut(s) 310, 733, 1090, 2095
SfcI CTRYAG 1 cut(s) 975
SfoI GGCGCC 1 cut(s) 58
SfuI TTCGAA 1 cut(s) 1254
SinI GGWCC 4 cut(s) 214, 454, 468, 905
SmiMI CAYNNNNRTG 4 cut(s) 245, 954, 1086, 1521
SpeI ACTAGT 1 cut(s) 2041
SphI GCATGC 1 cut(s) 1478
SseBI AGGCCT 1 cut(s) 1215
SsiI CCGC 9 cut(s) 263, 266, 368, 401, 426, 903, 1883, 1892, 1907
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 9 cut(s) 419, 632, 900, 1086, 1271, 2010, 2042, 2105, 2363
StuI AGGCCT 1 cut(s) 1215
StyD4I CCNGG 2 cut(s) 1659, 1780
StyI CCWWGG 5 cut(s) 52, 457, 512, 1643, 1860
TaaI ACNGT 2 cut(s) 247, 1789
TaiI ACGT 2 cut(s) 66, 315
TaqI TCGA 5 cut(s) 66, 234, 688, 1254, 2022
TatI WGTACW 3 cut(s) 189, 937, 2174
TauI GCSGC 3 cut(s) 265, 268, 1894
TfiI GAWTC 8 cut(s) 184, 532, 685, 822, 1094, 1430, 1856, 1918
Tru1I TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2018, 2154, 2229
Tru9I TTAA 9 cut(s) 393, 660, 860, 1106, 1130, 1512, 2018, 2154, 2229
TscAI CASTG 3 cut(s) 1546, 2215, 2265
TseFI GTSAC 4 cut(s) 438, 811, 895, 1385
TseI GCWGC 4 cut(s) 75, 1601, 1940, 2369
Tsp45I GTSAC 4 cut(s) 438, 811, 895, 1385
TspGWI ACGGA 2 cut(s) 1728, 2263
TspRI CASTG 3 cut(s) 1546, 2215, 2265
Van91I CCANNNNNTGG 1 cut(s) 1660
VpaK11BI GGWCC 4 cut(s) 214, 454, 468, 905
VspI ATTAAT 1 cut(s) 2018
XagI CCTNNNNNAGG 1 cut(s) 1177
XapI RAATTY 4 cut(s) 911, 960, 1483, 1831
XceI RCATGY 3 cut(s) 953, 1358, 1478
XcmI CCANNNNNNNNNTGG 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 1339
XmnI GAANNNNTTC 1 cut(s) 1685
XspI CTAG 9 cut(s) 419, 632, 900, 1086, 1271, 2010, 2042, 2105, 2363
ZraI GACGTC 1 cut(s) 313
ZrmI AGTACT 1 cut(s) 2176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.