Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000684

Physical Location & Seq

Genomic Coordinates Forward (+)

591249 .. 596955

Gene Structure 5707 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1007400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGCGAACTTCATGGGCAGATTTGGCTGCAAATTCTGCAGCTGAGAATGTAGGTTCTGGCTCTTCTGGCAATGTTGGTTCAGGGAGTGCTCCTGCTCCTGCTCCTGCTTCTAATCGGTCCACCTATGTCCCCCCACATCTCCGTAACCGTCCACCTTCCTCGGACCCACCTGCTCCAGCATATACTGCGCCGGTTACCAATGATCGAGGTGGCCCAGGTGGGTTCAGTGCACCTCGTTGGGGTGGTCCCAGAAACGACAACAGTCGAACTGGCTATGGTGGCAATGCTGGTCGTGGTGGAGGATGGGGAAGCAGGAGTGGTGGTTGGGATGGGAGAGCACGAGAAGTGAACCCCTTTAGAGATGATGATGAAACAGAGCAGCCGTTTAGTGAGCAAGAGAACAGTGGTATTAACTTTGATGCATACGAAGATATTCCGGTTGAGACAAGTGGCGATAATGTTCCCACTCCTGTGAATACCTTTGCAGATATTGACTTGGGGGACGCACTTAATAAAAATATTCAGAGGTGCAAATATGTGAAACCCACACCTGTGCAGCGTCATGCCATTCCTATCTCCCTTGCAGGCCGAGACTTGATGGCATGTGCCCAAACTGGTTCTGGAAAGACTGCTGCTTTCTGTTTCCCAATCATCAGTGGAATTATGAAGGGACAACCTGCACAGAGACCACCCCGTGGTGCACATACAGTTCATCCGCTTGCTCTCATTCTATCTCCTACTAGGGAGCTTTCAATTCAAATTCATGAGGAAGCCCGGAAATTTTCGTATCAAACAGGTGTTAGGGTGGTTGTTGCGTATGGAGGAGCACCAATTAACCAACAGCTGCGGGAGCTTGAAAGAGGTGTTGATATTCTTGTGGCAACTCCTGGAAGATTGGTGGATTTGCTGGAGAGAGCCAGGGTTTCGTTGCAGATGATTAGATATTTAGCCCTAGATGAGGCTGATCGAATGCTGGATATGGGTTTTGAACCACAAATAAGGAAAATTGTGGAGCAAATGGACATGCCTCCTCCAGGTATGCGACAGACTATGCTGTTCAGTGCCACTTTTCCGAAGGAAATACAGAGACTGGCCTCTGATTTTCTTGCAAAGTACATCTTTCTGGCGGTTGGAAGGGTGGGTTCTAGTACTGATTTGATTGTCCAAAGAGTGGAATTTGTGCATGAATCTGATAAAAGAAGTCACCTCATGGACCTTATTCATGCACAGAGGGCCAATGGCGCACAGGGCAAGCAAGCTCTGACATTAGTTTTTGTGGAGACAAAGAAGGGAGCTGATTCGCTGGAACATTGGCTGTGTATGAATGGTTTTCCTGCAACTACTATTCATGGTGACAGATCACAGCAGGAAAGAGAACAAGCATTGAGGTCATTTAAGAGTGGGAACACTCCGATCTTGGTGGCTACTGATGTGGCTGCACGTGGGCTAGACATTCCTCATGTTGCACATGTTGTCAACTTTGATCTTCCAAATGACATTGATGATTATGTTCACCGCATTGGTCGTACGGGACGAGCTGGGAAGTCTGGCTTGGCTACTGCCTTCTTTAATGAGAACAATTCATCACTTGCTAGGTCTTTGGCAGAATTGATGCAAGAATCAAATCAAGAAGTACCTGCTTGGCTGTCCCGGTACGCAGCTCGAGCTTCTTTTGGTGGTGGGAGGAACCGTCGTTCTGGGGGAGGCCGGTTTGGTGGCCGTGACTTCCGAAGGGATTCCTCTTTCAGCAGGGGTGGCAATGATTACTACGCTGGAGGTGGTGGGGGTAGCAGCGGTGGATATGGGGGTGCTTCTGGTGGATATGGGGGTGCTTCTGGAGGATATGGTGCTGGGGTGGCCAGTGCGTGGGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04622 ko05161 ko05203 map04622 map05161 map05203

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

625

Amino Acids

66.83

Weight (kDa)

8.54

Isoelectric Point (pI)

43.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	183 - 363	1.3e-49	DEAD/DEAH box helicase
Helicase_C	PF00271	399 - 514	1.1e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42520 AT2G42520 AT2G42520 AT3G58510 AT3G58510 AT3G58510 AT3G58570
fragaria_vesca	FvH4_1g03520 FvH4_1g03520 FvH4_1g03520 FvH4_3g45200 FvH4_3g45200 FvH4_3g45200 FvH4_6g21570
malus_domestica	MD02G1034300.v1.1 MD03G1007400.v1.1 MD11G1010700.v1.1 MD12G1053000.v1.1 MD14G1051400.v1.1 MD15G1175000.v1.1
prunus_persica	Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.7G084600_v2.0.a1 Prupe.7G240900_v2.0.a1
pyrus_communis	pycom02g02800 pycom03g00760 pycom11g00670 pycom12g04630 pycom14g04260 pycom15g15650
rosa_chinensis	RchiOBHm_Chr2g0088731 RchiOBHm_Chr2g0088781 RchiOBHm_Chr2g0088941 RchiOBHm_Chr3g0478811 RchiOBHm_Chr5g0082811
rosa_laevigata	RLG00000015980 RLG00000016349 RLG00000023623 RLG00000036991
rosa_multiflora	Rmu_co8138978.1_g000001 Rmu_co8475903.1_g000001 Rmu_sc0000965.1_g000010 Rmu_sc0001381.1_g000020 Rmu_sc0001519.1_g000001 Rmu_sc0005751.1_g000004 Rmu_sc0011683.1_g000004
rosa_roxburghii	Rroxscaffold_1G00001630 Rroxscaffold_2G00148710 Rroxscaffold_6G00403050
rosa_rugosa	Rorug01G0486600 Rorug03G0168400 Rorug05G0479900 Rorug05G0479900 Rorug05G0479900 Rorug05G0480000
rosa_samantha	Rh2AG041800 Rh2AG076200 Rh2BG040500 Rh2BG077600 Rh2CG041300 Rh2CG041900 Rh2CG079800 Rh2DG040900 Rh2DG074900 Rh3AG220200 Rh3BG252000 Rh3DG245100 Rh5AG530300 Rh5DG565600
rosa_wichuraiana	Rw2G003260 Rw2G006990 Rw3G019790 Rw5G049570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	178
Acc36I	ACCTGC	3 cut(s)	178, 685, 1645
AccB7I	CCANNNNNTGG	3 cut(s)	695, 1171, 1866
AciI	CCGC	5 cut(s)	716, 847, 1127, 1516, 1794
AcoI	YGGCCR	2 cut(s)	1717, 1857
AcsI	RAATTY	4 cut(s)	31, 759, 779, 1175
AcvI	CACGTG	1 cut(s)	1442
AdeI	CACNNNGTG	1 cut(s)	695
AfaI	GTAC	5 cut(s)	1115, 1150, 1528, 1635, 1655
AfiI	CCNNNNNNNGG	6 cut(s)	239, 695, 958, 1034, 1171, 1866
AflIII	ACRYGT	1 cut(s)	1468
AgsI	TTSAA	4 cut(s)	753, 758, 857, 989
AjnI	CCWGG	4 cut(s)	214, 886, 917, 1033
AjuI	GAANNNNNNNTTGG	2 cut(s)	1535, 1567
AleI	CACNNNNGTG	2 cut(s)	470, 551
AloI	GAACNNNNNNTCC	2 cut(s)	1678, 1710
AluBI	AGCT	9 cut(s)	41, 748, 844, 853, 1259, 1295, 1538, 1661, 1667
AluI	AGCT	9 cut(s)	41, 748, 844, 853, 1259, 1295, 1538, 1661, 1667
Alw21I	GWGCWC	5 cut(s)	91, 232, 340, 703, 829
Alw26I	GTCTC	5 cut(s)	437, 585, 679, 1081, 1274
Alw44I	GTGCAC	2 cut(s)	228, 699
AlwNI	CAGNNNCTG	1 cut(s)	1090
Ama87I	CYCGRG	1 cut(s)	1662
AoxI	GGCC	7 cut(s)	211, 586, 1092, 1233, 1705, 1717, 1857
ApaLI	GTGCAC	2 cut(s)	228, 699
ApeKI	GCWGC	9 cut(s)	26, 38, 379, 556, 632, 844, 1436, 1658, 1791
ApoI	RAATTY	4 cut(s)	31, 759, 779, 1175
Asp700I	GAANNNNTTC	2 cut(s)	432, 1735
AspLEI	GCGC	2 cut(s)	190, 1244
AspS9I	GGNCC	6 cut(s)	117, 163, 212, 245, 1213, 1233
AsuC2I	CCSGG	2 cut(s)	775, 1651
AsuHPI	GGTGA	3 cut(s)	1196, 1364, 1505
AvaI	CYCGRG	1 cut(s)	1662
AvaII	GGWCC	4 cut(s)	117, 163, 245, 1213
BaeGI	GKGCMC	3 cut(s)	232, 610, 703
BalI	TGGCCA	1 cut(s)	1859
BauI	CACGAG	1 cut(s)	339
BbrPI	CACGTG	1 cut(s)	1442
Bbv12I	GWGCWC	5 cut(s)	91, 232, 340, 703, 829
BbvI	GCAGC	9 cut(s)	13, 50, 391, 568, 619, 831, 1423, 1670, 1803
BccI	CCATC	3 cut(s)	297, 323, 592
BceAI	ACGGC	2 cut(s)	367, 1704
BciT130I	CCWGG	4 cut(s)	216, 888, 919, 1035
BcnI	CCSGG	2 cut(s)	775, 1651
BcoDI	GTCTC	5 cut(s)	437, 585, 679, 1081, 1274
BfaI	CTAG	5 cut(s)	741, 953, 1146, 1448, 1593
BfmI	CTRYAG	1 cut(s)	36
BfuAI	ACCTGC	3 cut(s)	178, 685, 1645
BisI	GCNGC	9 cut(s)	27, 39, 380, 557, 633, 845, 1437, 1659, 1792
BlsI	GCNGC	9 cut(s)	28, 40, 381, 558, 634, 846, 1438, 1660, 1793
BmcAI	AGTACT	1 cut(s)	1150
Bme1390I	CCNGG	6 cut(s)	216, 775, 888, 919, 1035, 1651
Bme18I	GGWCC	4 cut(s)	117, 163, 245, 1213
BmeT110I	CYCGRG	1 cut(s)	1662
BmgT120I	GGNCC	6 cut(s)	117, 163, 212, 245, 1213, 1233
BmiI	GGNNCC	3 cut(s)	165, 247, 1688
BmrFI	CCNGG	6 cut(s)	216, 775, 888, 919, 1035, 1651
BmsI	GCATC	2 cut(s)	409, 1602
BoxI	GACNNNNGTC	1 cut(s)	261
BpmI	CTGGAG	5 cut(s)	159, 929, 1017, 1794, 1857
BpuMI	CCSGG	2 cut(s)	775, 1651
BsaAI	YACGTR	1 cut(s)	1442
BsaI	GGTCTC	1 cut(s)	679
BsaJI	CCNNGG	4 cut(s)	159, 214, 694, 918
BsaWI	WCCGGW	1 cut(s)	436
BsaXI	ACNNNNNCTCC	4 cut(s)	307, 337, 1675, 1705
Bsc4I	CCNNNNNNNGG	6 cut(s)	239, 695, 958, 1034, 1171, 1866
Bse118I	RCCGGY	2 cut(s)	190, 1707
Bse1I	ACTGG	4 cut(s)	274, 619, 1095, 1860
Bse3DI	GCAATG	3 cut(s)	76, 289, 1765
BseBI	CCWGG	4 cut(s)	216, 888, 919, 1035
BseDI	CCNNGG	4 cut(s)	159, 214, 694, 918
BseGI	GGATG	3 cut(s)	308, 334, 712
BseLI	CCNNNNNNNGG	6 cut(s)	239, 695, 958, 1034, 1171, 1866
BseMI	GCAATG	3 cut(s)	76, 289, 1765
BseMII	CTCAG	1 cut(s)	33
BseNI	ACTGG	4 cut(s)	274, 619, 1095, 1860
BseRI	GAGGAG	2 cut(s)	837, 1020
BseSI	GKGCMC	3 cut(s)	232, 610, 703
BseXI	GCAGC	9 cut(s)	13, 50, 391, 568, 619, 831, 1423, 1670, 1803
BseYI	CCCAGC	2 cut(s)	1538, 1850
BsgI	GTGCAG	3 cut(s)	575, 663, 1422
BshFI	GGCC	7 cut(s)	213, 588, 1094, 1235, 1707, 1719, 1859
BsiHKAI	GWGCWC	5 cut(s)	91, 232, 340, 703, 829
BsiHKCI	CYCGRG	1 cut(s)	1662
BsiSI	CCGG	5 cut(s)	191, 437, 775, 1651, 1708
BsiWI	CGTACG	1 cut(s)	1526
BslFI	GGGAC	6 cut(s)	113, 231, 515, 684, 1545, 1633
BslI	CCNNNNNNNGG	6 cut(s)	239, 695, 958, 1034, 1171, 1866
BsmAI	GTCTC	5 cut(s)	437, 585, 679, 1081, 1274
BsmFI	GGGAC	6 cut(s)	113, 231, 515, 684, 1545, 1633
BsmI	GAATGC	1 cut(s)	975
BsnI	GGCC	7 cut(s)	213, 588, 1094, 1235, 1707, 1719, 1859
Bso31I	GGTCTC	1 cut(s)	679
BsoBI	CYCGRG	1 cut(s)	1662
Bsp1286I	GDGCHC	6 cut(s)	91, 232, 340, 610, 703, 829
Bsp143I	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
BspACI	CCGC	5 cut(s)	716, 847, 1127, 1516, 1794
BspANI	GGCC	7 cut(s)	213, 588, 1094, 1235, 1707, 1719, 1859
BspCNI	CTCAG	1 cut(s)	34
BspHI	TCATGA	1 cut(s)	763
BspLI	GGNNCC	3 cut(s)	165, 247, 1688
BspMAI	CTGCAG	1 cut(s)	40
BspMI	ACCTGC	3 cut(s)	178, 685, 1645
BspQI	GCTCTTC	1 cut(s)	67
BspTNI	GGTCTC	1 cut(s)	679
BsrDI	GCAATG	3 cut(s)	76, 289, 1765
BsrFI	RCCGGY	2 cut(s)	190, 1707
BsrI	ACTGG	4 cut(s)	274, 619, 1095, 1860
BssAI	RCCGGY	2 cut(s)	190, 1707
BssECI	CCNNGG	4 cut(s)	159, 214, 694, 918
BssMI	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
BssSI	CACGAG	1 cut(s)	339
Bst2BI	CACGAG	1 cut(s)	339
Bst2UI	CCWGG	4 cut(s)	216, 888, 919, 1035
Bst4CI	ACNGT	5 cut(s)	149, 263, 404, 709, 1691
Bst6I	CTCTTC	1 cut(s)	67
BstAPI	GCANNNNNTGC	2 cut(s)	35, 185
BstBAI	YACGTR	1 cut(s)	1442
BstC8I	GCNNGC	4 cut(s)	586, 720, 1253, 1257
BstDEI	CTNAG	1 cut(s)	42
BstDSI	CCRYGG	1 cut(s)	694
BstEII	GGTNACC	1 cut(s)	193
BstENI	CCTNNNNNAGG	2 cut(s)	956, 1032
BstF5I	GGATG	3 cut(s)	308, 334, 712
BstHHI	GCGC	2 cut(s)	190, 1244
BstKTI	GATC	5 cut(s)	205, 967, 1361, 1416, 1486
BstMAI	GTCTC	5 cut(s)	437, 585, 679, 1081, 1274
BstMBI	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
BstNI	CCWGG	4 cut(s)	216, 888, 919, 1035
BstNSI	RCATGY	3 cut(s)	606, 1027, 1472
BstPAI	GACNNNNGTC	1 cut(s)	261
BstPI	GGTNACC	1 cut(s)	193
BstSCI	CCNGG	6 cut(s)	214, 773, 886, 917, 1033, 1649
BstSFI	CTRYAG	1 cut(s)	36
BstSLI	GKGCMC	3 cut(s)	232, 610, 703
BstV1I	GCAGC	9 cut(s)	13, 50, 391, 568, 619, 831, 1423, 1670, 1803
BsuRI	GGCC	7 cut(s)	213, 588, 1094, 1235, 1707, 1719, 1859
BtgI	CCRYGG	1 cut(s)	694
BtsCI	GGATG	3 cut(s)	308, 334, 712
BtsIMutI	CAGTG	5 cut(s)	232, 409, 661, 1066, 1867
BveI	ACCTGC	3 cut(s)	178, 685, 1645
Cac8I	GCNNGC	4 cut(s)	586, 720, 1253, 1257
CaiI	CAGNNNCTG	1 cut(s)	1090
CciI	TCATGA	1 cut(s)	763
CfoI	GCGC	2 cut(s)	190, 1244
Cfr10I	RCCGGY	2 cut(s)	190, 1707
Cfr13I	GGNCC	6 cut(s)	117, 163, 212, 245, 1213, 1233
CseI	GACGC	2 cut(s)	512, 548
Csp6I	GTAC	5 cut(s)	1114, 1149, 1527, 1634, 1654
CviQI	GTAC	5 cut(s)	1114, 1149, 1527, 1634, 1654
DdeI	CTNAG	1 cut(s)	42
DpnI	GATC	5 cut(s)	204, 966, 1360, 1415, 1485
DpnII	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
DraIII	CACNNNGTG	1 cut(s)	695
EaeI	YGGCCR	2 cut(s)	1717, 1857
Eam1104I	CTCTTC	1 cut(s)	67
EarI	CTCTTC	1 cut(s)	67
Eco31I	GGTCTC	1 cut(s)	679
Eco47I	GGWCC	4 cut(s)	117, 163, 245, 1213
Eco72I	CACGTG	1 cut(s)	1442
Eco88I	CYCGRG	1 cut(s)	1662
Eco91I	GGTNACC	1 cut(s)	193
EcoNI	CCTNNNNNAGG	2 cut(s)	956, 1032
EcoO65I	GGTNACC	1 cut(s)	193
EcoRII	CCWGG	4 cut(s)	214, 886, 917, 1033
EcoT22I	ATGCAT	1 cut(s)	424
FalI	AAGNNNNNCTT	4 cut(s)	1103, 1135, 1535, 1567
FaqI	GGGAC	6 cut(s)	113, 231, 515, 684, 1545, 1633
FauI	CCCGC	1 cut(s)	840
Fnu4HI	GCNGC	9 cut(s)	27, 39, 380, 557, 633, 845, 1437, 1659, 1792
FokI	GGATG	3 cut(s)	315, 341, 699
Fsp4HI	GCNGC	9 cut(s)	27, 39, 380, 557, 633, 845, 1437, 1659, 1792
FspBI	CTAG	5 cut(s)	741, 953, 1146, 1448, 1593
GlaI	GCGC	2 cut(s)	189, 1243
GluI	GCNGC	9 cut(s)	27, 39, 380, 557, 633, 845, 1437, 1659, 1792
GsaI	CCCAGC	2 cut(s)	1542, 1854
GsuI	CTGGAG	5 cut(s)	159, 929, 1017, 1794, 1857
HaeIII	GGCC	7 cut(s)	213, 588, 1094, 1235, 1707, 1719, 1859
HapII	CCGG	5 cut(s)	191, 437, 775, 1651, 1708
HgaI	GACGC	2 cut(s)	512, 548
HhaI	GCGC	2 cut(s)	190, 1244
Hin6I	GCGC	2 cut(s)	188, 1242
HinP1I	GCGC	2 cut(s)	188, 1242
HincII	GTYRAC	1 cut(s)	1477
HindII	GTYRAC	1 cut(s)	1477
HinfI	GANTC	4 cut(s)	1187, 1298, 1619, 1736
HpaII	CCGG	5 cut(s)	191, 437, 775, 1651, 1708
HphI	GGTGA	3 cut(s)	1196, 1364, 1505
Hpy166II	GTNNAC	7 cut(s)	120, 152, 230, 349, 701, 1477, 1513
Hpy188I	TCNGA	8 cut(s)	163, 525, 1074, 1099, 1192, 1263, 1413, 1730
Hpy188III	TCNNGA	4 cut(s)	621, 764, 1628, 1836
Hpy8I	GTNNAC	7 cut(s)	120, 152, 230, 349, 701, 1477, 1513
Hpy99I	CGWCG	1 cut(s)	1695
HpyAV	CCTTC	7 cut(s)	165, 661, 1069, 1128, 1282, 1573, 1725
HpyCH4III	ACNGT	5 cut(s)	149, 263, 404, 709, 1691
HpyCH4IV	ACGT	1 cut(s)	1441
HpyF3I	CTNAG	1 cut(s)	42
HpySE526I	ACGT	1 cut(s)	1441
HspAI	GCGC	2 cut(s)	188, 1242
Kzo9I	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
LguI	GCTCTTC	1 cut(s)	67
LmnI	GCTCC	9 cut(s)	94, 100, 106, 178, 745, 824, 850, 1012, 1292
Lsp1109I	GCAGC	9 cut(s)	13, 50, 391, 568, 619, 831, 1423, 1670, 1803
LweI	GCATC	2 cut(s)	409, 1602
MaeI	CTAG	5 cut(s)	741, 953, 1146, 1448, 1593
MaeII	ACGT	1 cut(s)	1441
MaeIII	GTNAC	5 cut(s)	143, 193, 1202, 1352, 1721
MalI	GATC	5 cut(s)	204, 966, 1360, 1415, 1485
MboI	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
MboII	GAAGA	4 cut(s)	54, 440, 903, 1478
MhlI	GDGCHC	6 cut(s)	91, 232, 340, 610, 703, 829
MlsI	TGGCCA	1 cut(s)	1859
MluNI	TGGCCA	1 cut(s)	1859
MmeI	TCCRAC	1 cut(s)	1111
Mox20I	TGGCCA	1 cut(s)	1859
Mph1103I	ATGCAT	1 cut(s)	424
MroXI	GAANNNNTTC	2 cut(s)	432, 1735
MscI	TGGCCA	1 cut(s)	1859
MseI	TTAA	5 cut(s)	411, 510, 834, 1395, 1569
MslI	CAYNNNNRTG	2 cut(s)	470, 551
Msp20I	TGGCCA	1 cut(s)	1859
MspA1I	CMGCKG	3 cut(s)	41, 844, 1794
MspI	CCGG	5 cut(s)	191, 437, 775, 1651, 1708
MspR9I	CCNGG	6 cut(s)	216, 775, 888, 919, 1035, 1651
Mva1269I	GAATGC	1 cut(s)	975
MvaI	CCWGG	4 cut(s)	216, 888, 919, 1035
NciI	CCSGG	2 cut(s)	775, 1651
NdeII	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
NlaIV	GGNNCC	3 cut(s)	165, 247, 1688
NmeAIII	GCCGAG	1 cut(s)	614
NmuCI	GTSAC	3 cut(s)	1202, 1352, 1721
NsiI	ATGCAT	1 cut(s)	424
NspI	RCATGY	3 cut(s)	606, 1027, 1472
OliI	CACNNNNGTG	2 cut(s)	470, 551
PaeR7I	CTCGAG	1 cut(s)	1662
PagI	TCATGA	1 cut(s)	763
PaqCI	CACCTGC	1 cut(s)	178
PciI	ACATGT	1 cut(s)	1468
PciSI	GCTCTTC	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	1 cut(s)	1531
PctI	GAATGC	1 cut(s)	975
PdmI	GAANNNNTTC	2 cut(s)	432, 1735
PfeI	GAWTC	4 cut(s)	1187, 1298, 1619, 1736
Pfl23II	CGTACG	1 cut(s)	1526
PflMI	CCANNNNNTGG	3 cut(s)	695, 1171, 1866
PfoI	TCCNGGA	1 cut(s)	886
PkrI	GCNGC	9 cut(s)	28, 40, 381, 558, 634, 846, 1438, 1660, 1793
PmaCI	CACGTG	1 cut(s)	1442
PmlI	CACGTG	1 cut(s)	1442
Ppu21I	YACGTR	1 cut(s)	1442
PscI	ACATGT	1 cut(s)	1468
PshAI	GACNNNNGTC	1 cut(s)	261
Psp6I	CCWGG	4 cut(s)	214, 886, 917, 1033
PspCI	CACGTG	1 cut(s)	1442
PspEI	GGTNACC	1 cut(s)	193
PspFI	CCCAGC	2 cut(s)	1538, 1850
PspGI	CCWGG	4 cut(s)	214, 886, 917, 1033
PspLI	CGTACG	1 cut(s)	1526
PspN4I	GGNNCC	3 cut(s)	165, 247, 1688
PspPI	GGNCC	6 cut(s)	117, 163, 212, 245, 1213, 1233
PspXI	VCTCGAGB	1 cut(s)	1662
PstI	CTGCAG	1 cut(s)	40
PstNI	CAGNNNCTG	1 cut(s)	1090
PvuII	CAGCTG	2 cut(s)	41, 844
RsaI	GTAC	5 cut(s)	1115, 1150, 1528, 1635, 1655
RsaNI	GTAC	5 cut(s)	1114, 1149, 1527, 1634, 1654
RseI	CAYNNNNRTG	2 cut(s)	470, 551
SapI	GCTCTTC	1 cut(s)	67
SaqAI	TTAA	5 cut(s)	411, 510, 834, 1395, 1569
SatI	GCNGC	9 cut(s)	27, 39, 380, 557, 633, 845, 1437, 1659, 1792
Sau3AI	GATC	5 cut(s)	202, 964, 1358, 1413, 1483
Sau96I	GGNCC	6 cut(s)	117, 163, 212, 245, 1213, 1233
ScaI	AGTACT	1 cut(s)	1150
ScrFI	CCNGG	6 cut(s)	216, 775, 888, 919, 1035, 1651
SduI	GDGCHC	6 cut(s)	91, 232, 340, 610, 703, 829
SfaNI	GCATC	2 cut(s)	409, 1602
SfcI	CTRYAG	1 cut(s)	36
Sfr274I	CTCGAG	1 cut(s)	1662
SinI	GGWCC	4 cut(s)	117, 163, 245, 1213
SlaI	CTCGAG	1 cut(s)	1662
SmiMI	CAYNNNNRTG	2 cut(s)	470, 551
SmlI	CTYRAG	1 cut(s)	1662
SmoI	CTYRAG	1 cut(s)	1662
SsiI	CCGC	5 cut(s)	716, 847, 1127, 1516, 1794
SspI	AATATT	1 cut(s)	520
SspMI	CTAG	5 cut(s)	741, 953, 1146, 1448, 1593
StyD4I	CCNGG	6 cut(s)	214, 773, 886, 917, 1033, 1649
TaaI	ACNGT	5 cut(s)	149, 263, 404, 709, 1691
TaiI	ACGT	1 cut(s)	1444
TaqI	TCGA	4 cut(s)	205, 265, 967, 1663
TaqII	GACCGA	1 cut(s)	105
TatI	WGTACW	2 cut(s)	1113, 1148
TfiI	GAWTC	4 cut(s)	1187, 1298, 1619, 1736
Tru1I	TTAA	5 cut(s)	411, 510, 834, 1395, 1569
Tru9I	TTAA	5 cut(s)	411, 510, 834, 1395, 1569
TscAI	CASTG	5 cut(s)	232, 409, 661, 1066, 1867
TseFI	GTSAC	3 cut(s)	1202, 1352, 1721
TseI	GCWGC	9 cut(s)	26, 38, 379, 556, 632, 844, 1436, 1658, 1791
Tsp45I	GTSAC	3 cut(s)	1202, 1352, 1721
TspDTI	ATGAA	9 cut(s)	384, 680, 701, 752, 1200, 1211, 1337, 1337, 1572
TspGWI	ACGGA	1 cut(s)	131
TspRI	CASTG	5 cut(s)	232, 409, 661, 1066, 1867
Van91I	CCANNNNNTGG	3 cut(s)	695, 1171, 1866
VneI	GTGCAC	2 cut(s)	228, 699
VpaK11BI	GGWCC	4 cut(s)	117, 163, 245, 1213
XagI	CCTNNNNNAGG	2 cut(s)	956, 1032
XapI	RAATTY	4 cut(s)	31, 759, 779, 1175
XceI	RCATGY	3 cut(s)	606, 1027, 1472
XcmI	CCANNNNNNNNNTGG	1 cut(s)	617
XhoI	CTCGAG	1 cut(s)	1662
XmnI	GAANNNNTTC	2 cut(s)	432, 1735
XspI	CTAG	5 cut(s)	741, 953, 1146, 1448, 1593
ZrmI	AGTACT	1 cut(s)	1150
Zsp2I	ATGCAT	1 cut(s)	424

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.