Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000684

Physical Location & Seq

Genomic Coordinates Forward (+)

5930762 .. 5936307

Gene Structure 5546 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1053000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1872 bp

ATGACAACTTCATGGGCGGATTCTGTCGCAAATTCTCAAGGAGAGAATCCGACTATCGGTTCATCTGCAAATAACAGCTCATCTCGCCCTGCACGATCAAGTTATGTCCCGCCACATCTTCGTAATAGGCCGCCGTCTTCTGATGCCACGGCTCCTTCACATCCTGCCCCATTACCAGCCAATGACCGCGTAGGTTACAATGGGCCTGCACCTGGCCCTGCCTGGGGTGCTGGCAGTTCTAGGCATGATGTTGGGCGTCCGGGATATGGTTCCAGTGGTGGTCGAGGTGGTGGTGGTTGGAACAATAGAAGTGGGGGGTGGGACCGTGGGAGGGAGCGGGAAGTAAACCCTTTTGGCGATGAAGACATCTCGGAACCGACATTTAGTGATCAAGAAAACACAGGCATTAACTTTGATGCTTATGAGGATATCCCAGTGGAAACTAGTGGGGATAATGTGCCACCACCTGTGAATACTTTTGCAGAGATAGACTTAGGGGAGGCATTGAATTTAAATATTCGAAGGTGCAGGTATGTGAAGCCAACTCCTGTTCAGCGTCATGCAATACCTATTTCTCTGGGAGGTAGGGATTTGATGGCTTGTGCTCAGACGGGTTCAGGGAAGACAGCTGCTTTCTGCTTTCCAATTATTAGTGGAATCATGCTAGGGCAGTATGCTCAGAGACCTCGTGGAGCACGTACTGTGTATCCTCTTGCTCTTATTCTCTCCCCGACCAGAGAACTCTCTTGCCAGATACATGAAGAAACTAGAAAGTTTGCTTATCAAACCGGTGTCAAGGTGGTAGTTGCTTATGGAGGTGCACCAATAAATCAACAGTTGAGAGAGCTTGAGAGAGGAGTTGATATTCTTGTGGCAACTCCGGGACGATTGGTAGATTTGCTTGAGAGGGCAAGAGTGTCGTTGCAGATGATCAGATATTTGGCTCTTGATGAGGCAGATAGGATGCTGGACATGGGTTTTGAGCCTCAAATTAGGAAGATAGTGGAAAAGATGGACATGCCTCCGCCAGGTTTGAGACAGACCATGCTGTTTAGTGCTACGTTTCCAAAGGAGATACAGCGACTGGCATCAGATTTTCTTTCAAATTATATATTTTTGGCTGTTGGAAGGGTTGGTTCAAGTACTGATTTAATTGTTCAAAGAGTTGAATTTGTTCATGAGTCTGATAAACGAAGTCATCTCATGGACCTCCTTCATGCACAGAGGGAAAATGGAGCTCATGGCAAGCAATCTCTGACGTTAGTTTTTGTGGAGACAAAGAAGGGAGCTGATGCATTGGAACACTGGTTGTGCATGAATGGGTTTCCAGCAACTACTATTCATGGTGACAGAACACAACAGGAAAGAGAACATGCATTAAGATCATTCAAGAGCGGAAATACACCAATATTAGTGGCAACAGATGTGGCAGCACGTGGTCTTGACATCCCCCATGTAGCTCATGTGGTAAATTTTGATCTTCCAAATGACGTTGATGATTATGTTCACCGGATAGGTCGGACAGGGCGAGCCGGCAAATCAGGACTGGCAACTGCTTTCTTTAACGAGAACAATCTCTCATTGGCAAAACCACTCGCTGATCTAATGCAAGAAGCAAATCAGGAAGTACCTGCTTGGCTTGCTCGTTATGCATCAAGGGCTTCTTATGGCGGAGGAGGCAGAAACCGACGATCAGGGGGAGGTCGGTTTGGTGGCCGTGATTTTAGAAGGGACGGCTCTTTCAGTAGGGGTTCAGATTTTTATGGTGGAGGAAATGGTGGTGGATATGGCAACTCTGGTGGCTATGGTGGCGGCTCTGGGGGTTATGGTCCGGGTAATGGTGTTAACTTCGTTTGGTGGAAAGCACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005777 GO:0005886 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016020 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042579 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko04622 ko05161 ko05203 map04622 map05161 map05203

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

624

Amino Acids

67.38

Weight (kDa)

8.47

Isoelectric Point (pI)

40.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	182 - 361	6.4e-48	DEAD/DEAH box helicase
Helicase_C	PF00271	397 - 512	1.3e-31	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42520 AT2G42520 AT2G42520 AT3G58510 AT3G58510 AT3G58510 AT3G58570
fragaria_vesca	FvH4_1g03520 FvH4_1g03520 FvH4_1g03520 FvH4_3g45200 FvH4_3g45200 FvH4_3g45200 FvH4_6g21570
malus_domestica	MD02G1034300.v1.1 MD03G1007400.v1.1 MD11G1010700.v1.1 MD12G1053000.v1.1 MD14G1051400.v1.1 MD15G1175000.v1.1
prunus_persica	Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.7G084600_v2.0.a1 Prupe.7G240900_v2.0.a1
pyrus_communis	pycom02g02800 pycom03g00760 pycom11g00670 pycom12g04630 pycom14g04260 pycom15g15650
rosa_chinensis	RchiOBHm_Chr2g0088731 RchiOBHm_Chr2g0088781 RchiOBHm_Chr2g0088941 RchiOBHm_Chr3g0478811 RchiOBHm_Chr5g0082811
rosa_laevigata	RLG00000015980 RLG00000016349 RLG00000023623 RLG00000036991
rosa_multiflora	Rmu_co8138978.1_g000001 Rmu_co8475903.1_g000001 Rmu_sc0000965.1_g000010 Rmu_sc0001381.1_g000020 Rmu_sc0001519.1_g000001 Rmu_sc0005751.1_g000004 Rmu_sc0011683.1_g000004
rosa_roxburghii	Rroxscaffold_1G00001630 Rroxscaffold_2G00148710 Rroxscaffold_6G00403050
rosa_rugosa	Rorug01G0486600 Rorug03G0168400 Rorug05G0479900 Rorug05G0479900 Rorug05G0479900 Rorug05G0480000
rosa_samantha	Rh2AG041800 Rh2AG076200 Rh2BG040500 Rh2BG077600 Rh2CG041300 Rh2CG041900 Rh2CG079800 Rh2DG040900 Rh2DG074900 Rh3AG220200 Rh3BG252000 Rh3DG245100 Rh5AG530300 Rh5DG565600
rosa_wichuraiana	Rw2G003260 Rw2G006990 Rw3G019790 Rw5G049570

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	519, 1639
AccBSI	CCGCTC	2 cut(s)	337, 1395
AccII	CGCG	1 cut(s)	189
AciI	CCGC	9 cut(s)	17, 110, 131, 187, 337, 1025, 1395, 1671, 1812
AcoI	YGGCCR	1 cut(s)	1714
AcsI	RAATTY	4 cut(s)	31, 508, 1169, 1471
AcvI	CACGTG	1 cut(s)	1436
AcyI	GRCGYC	1 cut(s)	256
AfaI	GTAC	3 cut(s)	700, 1144, 1629
AfiI	CCNNNNNNNGG	8 cut(s)	56, 212, 223, 224, 266, 331, 1028, 1838
AgeI	ACCGGT	1 cut(s)	788
AgsI	TTSAA	6 cut(s)	508, 1104, 1140, 1160, 1169, 1390
AhlI	ACTAGT	1 cut(s)	443
AjnI	CCWGG	3 cut(s)	211, 221, 1027
AluBI	AGCT	6 cut(s)	78, 629, 847, 1238, 1289, 1460
AluI	AGCT	6 cut(s)	78, 629, 847, 1238, 1289, 1460
Alw21I	GWGCWC	4 cut(s)	607, 697, 823, 1240
Alw26I	GTCTC	3 cut(s)	676, 1030, 1268
Alw44I	GTGCAC	1 cut(s)	819
AlwNI	CAGNNNCTG	1 cut(s)	1084
AoxI	GGCC	4 cut(s)	128, 203, 214, 1714
ApaLI	GTGCAC	1 cut(s)	819
ApeKI	GCWGC	2 cut(s)	629, 1430
ApoI	RAATTY	4 cut(s)	31, 508, 1169, 1471
ArsI	GACNNNNNNTTYG	2 cut(s)	962, 994
AsiGI	ACCGGT	1 cut(s)	788
Asp700I	GAANNNNTTC	1 cut(s)	1173
AspS9I	GGNCC	5 cut(s)	203, 215, 322, 1207, 1829
AsuC2I	CCSGG	3 cut(s)	261, 882, 1833
AsuHPI	GGTGA	2 cut(s)	1358, 1499
AsuII	TTCGAA	1 cut(s)	520
AvaII	GGWCC	3 cut(s)	322, 1207, 1829
BaeGI	GKGCMC	1 cut(s)	823
BanII	GRGCYC	1 cut(s)	1240
BauI	CACGAG	1 cut(s)	687
BbrPI	CACGTG	1 cut(s)	1436
BbsI	GAAGAC	3 cut(s)	129, 369, 629
Bbv12I	GWGCWC	4 cut(s)	607, 697, 823, 1240
BbvI	GCAGC	2 cut(s)	616, 1442
BccI	CCATC	2 cut(s)	589, 1006
BceAI	ACGGC	4 cut(s)	118, 165, 1701, 1750
BcgI	CGANNNNNNTGC	2 cut(s)	900, 934
BciT130I	CCWGG	3 cut(s)	213, 223, 1029
BciVI	GTATCC	1 cut(s)	717
BclI	TGATCA	2 cut(s)	388, 930
BcnI	CCSGG	3 cut(s)	261, 882, 1833
BcoDI	GTCTC	3 cut(s)	676, 1030, 1268
BcuI	ACTAGT	1 cut(s)	443
BfaI	CTAG	4 cut(s)	240, 444, 665, 768
BfuAI	ACCTGC	2 cut(s)	519, 1639
BfuI	GTATCC	1 cut(s)	717
BisI	GCNGC	4 cut(s)	131, 630, 1431, 1813
BlsI	GCNGC	4 cut(s)	132, 631, 1432, 1814
BmcAI	AGTACT	1 cut(s)	1144
Bme1390I	CCNGG	6 cut(s)	213, 223, 261, 882, 1029, 1833
Bme18I	GGWCC	3 cut(s)	322, 1207, 1829
BmgT120I	GGNCC	5 cut(s)	203, 215, 322, 1207, 1829
BmiI	GGNNCC	4 cut(s)	153, 271, 323, 375
BmrFI	CCNGG	6 cut(s)	213, 223, 261, 882, 1029, 1833
BmrI	ACTGGG	1 cut(s)	428
BmsI	GCATC	6 cut(s)	133, 406, 954, 1097, 1282, 1661
BmuI	ACTGGG	1 cut(s)	428
BpiI	GAAGAC	3 cut(s)	129, 369, 629
Bpu14I	TTCGAA	1 cut(s)	520
BpuEI	CTTGAG	3 cut(s)	21, 869, 923
BpuMI	CCSGG	3 cut(s)	261, 882, 1833
BsaAI	YACGTR	2 cut(s)	698, 1436
BsaHI	GRCGYC	1 cut(s)	256
BsaI	GGTCTC	1 cut(s)	676
BsaJI	CCNNGG	3 cut(s)	147, 222, 325
BsaWI	WCCGGW	2 cut(s)	788, 1509
Bsc4I	CCNNNNNNNGG	8 cut(s)	56, 212, 223, 224, 266, 331, 1028, 1838
Bse118I	RCCGGY	2 cut(s)	788, 1532
Bse1I	ACTGG	5 cut(s)	273, 434, 1089, 1310, 1551
BseBI	CCWGG	3 cut(s)	213, 223, 1029
BseDI	CCNNGG	3 cut(s)	147, 222, 325
BseGI	GGATG	3 cut(s)	160, 969, 1446
BseLI	CCNNNNNNNGG	8 cut(s)	56, 212, 223, 224, 266, 331, 1028, 1838
BseMII	CTCAG	2 cut(s)	620, 692
BseNI	ACTGG	5 cut(s)	273, 434, 1089, 1310, 1551
BseRI	GAGGAG	2 cut(s)	870, 1689
BseSI	GKGCMC	1 cut(s)	823
BseXI	GCAGC	2 cut(s)	616, 1442
BsgI	GTGCAG	3 cut(s)	75, 192, 547
Bsh1236I	CGCG	1 cut(s)	189
BshFI	GGCC	4 cut(s)	130, 205, 216, 1716
BshTI	ACCGGT	1 cut(s)	788
BsiHKAI	GWGCWC	4 cut(s)	607, 697, 823, 1240
BsiSI	CCGG	6 cut(s)	260, 789, 881, 1510, 1533, 1832
BslFI	GGGAC	4 cut(s)	92, 335, 897, 1745
BslI	CCNNNNNNNGG	8 cut(s)	56, 212, 223, 224, 266, 331, 1028, 1838
BsmAI	GTCTC	3 cut(s)	676, 1030, 1268
BsmFI	GGGAC	4 cut(s)	92, 335, 897, 1745
BsnI	GGCC	4 cut(s)	130, 205, 216, 1716
Bso31I	GGTCTC	1 cut(s)	676
Bsp119I	TTCGAA	1 cut(s)	520
Bsp1286I	GDGCHC	4 cut(s)	607, 697, 823, 1240
Bsp143I	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
BspACI	CCGC	9 cut(s)	17, 110, 131, 187, 337, 1025, 1395, 1671, 1812
BspANI	GGCC	4 cut(s)	130, 205, 216, 1716
BspCNI	CTCAG	2 cut(s)	619, 691
BspFNI	CGCG	1 cut(s)	189
BspHI	TCATGA	1 cut(s)	1177
BspLI	GGNNCC	4 cut(s)	153, 271, 323, 375
BspMI	ACCTGC	2 cut(s)	519, 1639
BspT104I	TTCGAA	1 cut(s)	520
BspTNI	GGTCTC	1 cut(s)	676
BsrBI	CCGCTC	2 cut(s)	337, 1395
BsrFI	RCCGGY	2 cut(s)	788, 1532
BsrI	ACTGG	5 cut(s)	273, 434, 1089, 1310, 1551
BssAI	RCCGGY	2 cut(s)	788, 1532
BssECI	CCNNGG	3 cut(s)	147, 222, 325
BssMI	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
BssNI	GRCGYC	1 cut(s)	256
BssSI	CACGAG	1 cut(s)	687
Bst2BI	CACGAG	1 cut(s)	687
Bst2UI	CCWGG	3 cut(s)	213, 223, 1029
Bst4CI	ACNGT	3 cut(s)	326, 703, 837
BstACI	GRCGYC	1 cut(s)	256
BstBAI	YACGTR	2 cut(s)	698, 1436
BstBI	TTCGAA	1 cut(s)	520
BstC8I	GCNNGC	6 cut(s)	207, 232, 1247, 1530, 1534, 1641
BstDEI	CTNAG	3 cut(s)	493, 606, 678
BstDSI	CCRYGG	2 cut(s)	147, 325
BstENI	CCTNNNNNAGG	1 cut(s)	1026
BstF5I	GGATG	3 cut(s)	160, 969, 1446
BstFNI	CGCG	1 cut(s)	189
BstKTI	GATC	7 cut(s)	98, 391, 933, 1385, 1480, 1603, 1694
BstMAI	GTCTC	3 cut(s)	676, 1030, 1268
BstMBI	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
BstMWI	GCNNNNNNNGC	7 cut(s)	84, 227, 1640, 1649, 1658, 1677, 1809
BstNI	CCWGG	3 cut(s)	213, 223, 1029
BstNSI	RCATGY	2 cut(s)	1021, 1376
BstSCI	CCNGG	6 cut(s)	211, 221, 259, 880, 1027, 1831
BstSLI	GKGCMC	1 cut(s)	823
BstUI	CGCG	1 cut(s)	189
BstV1I	GCAGC	2 cut(s)	616, 1442
BstV2I	GAAGAC	3 cut(s)	129, 369, 629
BsuI	GTATCC	1 cut(s)	717
BsuRI	GGCC	4 cut(s)	130, 205, 216, 1716
BtgI	CCRYGG	2 cut(s)	147, 325
BtgZI	GCGATG	1 cut(s)	372
BtsCI	GGATG	3 cut(s)	160, 969, 1446
BtsIMutI	CAGTG	3 cut(s)	280, 441, 1303
BveI	ACCTGC	2 cut(s)	519, 1639
Cac8I	GCNNGC	6 cut(s)	207, 232, 1247, 1530, 1534, 1641
CaiI	CAGNNNCTG	1 cut(s)	1084
CciI	TCATGA	1 cut(s)	1177
Cfr10I	RCCGGY	2 cut(s)	788, 1532
Cfr13I	GGNCC	5 cut(s)	203, 215, 322, 1207, 1829
CseI	GACGC	2 cut(s)	245, 545
Csp6I	GTAC	3 cut(s)	699, 1143, 1628
CspAI	ACCGGT	1 cut(s)	788
CspCI	CAANNNNNGTGG	8 cut(s)	292, 327, 1395, 1407, 1430, 1442, 1780, 1815
CviQI	GTAC	3 cut(s)	699, 1143, 1628
DdeI	CTNAG	3 cut(s)	493, 606, 678
DpnI	GATC	7 cut(s)	97, 390, 932, 1384, 1479, 1602, 1693
DpnII	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
DraI	TTTAAA	1 cut(s)	513
EaeI	YGGCCR	1 cut(s)	1714
EciI	GGCGGA	3 cut(s)	32, 1014, 1686
Ecl136II	GAGCTC	1 cut(s)	1238
Eco24I	GRGCYC	1 cut(s)	1240
Eco31I	GGTCTC	1 cut(s)	676
Eco32I	GATATC	1 cut(s)	430
Eco47I	GGWCC	3 cut(s)	322, 1207, 1829
Eco53kI	GAGCTC	1 cut(s)	1238
Eco72I	CACGTG	1 cut(s)	1436
EcoICRI	GAGCTC	1 cut(s)	1238
EcoNI	CCTNNNNNAGG	1 cut(s)	1026
EcoRII	CCWGG	3 cut(s)	211, 221, 1027
EcoRV	GATATC	1 cut(s)	430
EcoT22I	ATGCAT	3 cut(s)	1297, 1378, 1654
EcoT38I	GRGCYC	1 cut(s)	1240
FalI	AAGNNNNNCTT	2 cut(s)	1648, 1680
FaqI	GGGAC	4 cut(s)	92, 335, 897, 1745
FauI	CCCGC	2 cut(s)	117, 330
FbaI	TGATCA	2 cut(s)	388, 930
Fnu4HI	GCNGC	4 cut(s)	131, 630, 1431, 1813
FokI	GGATG	3 cut(s)	147, 976, 1433
FriOI	GRGCYC	1 cut(s)	1240
Fsp4HI	GCNGC	4 cut(s)	131, 630, 1431, 1813
FspBI	CTAG	4 cut(s)	240, 444, 665, 768
GluI	GCNGC	4 cut(s)	131, 630, 1431, 1813
HaeIII	GGCC	4 cut(s)	130, 205, 216, 1716
HapII	CCGG	6 cut(s)	260, 789, 881, 1510, 1533, 1832
HgaI	GACGC	2 cut(s)	245, 545
Hin1I	GRCGYC	1 cut(s)	256
HincII	GTYRAC	1 cut(s)	1846
HindII	GTYRAC	1 cut(s)	1846
HinfI	GANTC	4 cut(s)	20, 46, 657, 1181
HpaI	GTTAAC	1 cut(s)	1846
HpaII	CCGG	6 cut(s)	260, 789, 881, 1510, 1533, 1832
HphI	GGTGA	2 cut(s)	1358, 1499
Hpy166II	GTNNAC	4 cut(s)	346, 821, 1507, 1846
Hpy188III	TCNNGA	7 cut(s)	392, 947, 1178, 1390, 1442, 1542, 1622
Hpy8I	GTNNAC	4 cut(s)	346, 821, 1507, 1846
Hpy99I	CGWCG	1 cut(s)	1692
HpyAV	CCTTC	6 cut(s)	165, 516, 1122, 1223, 1276, 1722
HpyCH4III	ACNGT	3 cut(s)	326, 703, 837
HpyCH4IV	ACGT	5 cut(s)	697, 1061, 1259, 1435, 1491
HpyF10VI	GCNNNNNNNGC	7 cut(s)	84, 227, 1640, 1649, 1658, 1677, 1809
HpyF3I	CTNAG	3 cut(s)	493, 606, 678
HpySE526I	ACGT	5 cut(s)	697, 1061, 1259, 1435, 1491
Hsp92I	GRCGYC	1 cut(s)	256
KroI	GCCGGC	1 cut(s)	1532
KroNI	GCCGGC	1 cut(s)	1534
Ksp22I	TGATCA	2 cut(s)	388, 930
KspAI	GTTAAC	1 cut(s)	1846
Kzo9I	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
LmnI	GCTCC	5 cut(s)	157, 334, 692, 1235, 1286
Lsp1109I	GCAGC	2 cut(s)	616, 1442
LweI	GCATC	6 cut(s)	133, 406, 954, 1097, 1282, 1661
MaeI	CTAG	4 cut(s)	240, 444, 665, 768
MaeII	ACGT	5 cut(s)	697, 1061, 1259, 1435, 1491
MaeIII	GTNAC	2 cut(s)	194, 1346
MalI	GATC	7 cut(s)	97, 390, 932, 1384, 1479, 1602, 1693
MbiI	CCGCTC	2 cut(s)	337, 1395
MboI	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
MboII	GAAGA	7 cut(s)	110, 129, 374, 634, 773, 1009, 1472
MhlI	GDGCHC	4 cut(s)	607, 697, 823, 1240
MluCI	AATT	8 cut(s)	31, 508, 645, 990, 1105, 1152, 1169, 1471
MlyI	GAGTC	1 cut(s)	1190
MmeI	TCCRAC	4 cut(s)	74, 278, 1105, 1499
Mph1103I	ATGCAT	3 cut(s)	1297, 1378, 1654
MroNI	GCCGGC	1 cut(s)	1532
MroXI	GAANNNNTTC	1 cut(s)	1173
MseI	TTAA	6 cut(s)	408, 512, 1151, 1379, 1563, 1845
MspA1I	CMGCKG	1 cut(s)	629
MspI	CCGG	6 cut(s)	260, 789, 881, 1510, 1533, 1832
MspR9I	CCNGG	6 cut(s)	213, 223, 261, 882, 1029, 1833
MvaI	CCWGG	3 cut(s)	213, 223, 1029
MvnI	CGCG	1 cut(s)	189
MwoI	GCNNNNNNNGC	7 cut(s)	84, 227, 1640, 1649, 1658, 1677, 1809
NaeI	GCCGGC	1 cut(s)	1534
NciI	CCSGG	3 cut(s)	261, 882, 1833
NdeII	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
NgoMIV	GCCGGC	1 cut(s)	1532
NlaIV	GGNNCC	4 cut(s)	153, 271, 323, 375
NmuCI	GTSAC	1 cut(s)	1346
NsiI	ATGCAT	3 cut(s)	1297, 1378, 1654
NspI	RCATGY	2 cut(s)	1021, 1376
NspV	TTCGAA	1 cut(s)	520
PagI	TCATGA	1 cut(s)	1177
PcsI	WCGNNNNNNNCGW	3 cut(s)	91, 694, 1525
PdiI	GCCGGC	1 cut(s)	1534
PdmI	GAANNNNTTC	1 cut(s)	1173
PfeI	GAWTC	3 cut(s)	20, 46, 657
PfoI	TCCNGGA	2 cut(s)	259, 880
PinAI	ACCGGT	1 cut(s)	788
PkrI	GCNGC	4 cut(s)	132, 631, 1432, 1814
PleI	GAGTC	1 cut(s)	1189
PmaCI	CACGTG	1 cut(s)	1436
PmlI	CACGTG	1 cut(s)	1436
PpsI	GAGTC	1 cut(s)	1189
Ppu21I	YACGTR	2 cut(s)	698, 1436
Psp124BI	GAGCTC	1 cut(s)	1240
Psp6I	CCWGG	3 cut(s)	211, 221, 1027
PspCI	CACGTG	1 cut(s)	1436
PspGI	CCWGG	3 cut(s)	211, 221, 1027
PspN4I	GGNNCC	4 cut(s)	153, 271, 323, 375
PspPI	GGNCC	5 cut(s)	203, 215, 322, 1207, 1829
PstNI	CAGNNNCTG	1 cut(s)	1084
PvuII	CAGCTG	1 cut(s)	629
RsaI	GTAC	3 cut(s)	700, 1144, 1629
RsaNI	GTAC	3 cut(s)	699, 1143, 1628
SacI	GAGCTC	1 cut(s)	1240
SaqAI	TTAA	6 cut(s)	408, 512, 1151, 1379, 1563, 1845
SatI	GCNGC	4 cut(s)	131, 630, 1431, 1813
Sau3AI	GATC	7 cut(s)	95, 388, 930, 1382, 1477, 1600, 1691
Sau96I	GGNCC	5 cut(s)	203, 215, 322, 1207, 1829
ScaI	AGTACT	1 cut(s)	1144
SchI	GAGTC	1 cut(s)	1190
ScrFI	CCNGG	6 cut(s)	213, 223, 261, 882, 1029, 1833
SduI	GDGCHC	4 cut(s)	607, 697, 823, 1240
SfaNI	GCATC	6 cut(s)	133, 406, 954, 1097, 1282, 1661
SfuI	TTCGAA	1 cut(s)	520
SinI	GGWCC	3 cut(s)	322, 1207, 1829
SmiI	ATTTAAAT	1 cut(s)	513
SmlI	CTYRAG	3 cut(s)	36, 848, 902
SmoI	CTYRAG	3 cut(s)	36, 848, 902
SpeI	ACTAGT	1 cut(s)	443
Sse9I	AATT	8 cut(s)	31, 508, 645, 990, 1105, 1152, 1169, 1471
SsiI	CCGC	9 cut(s)	17, 110, 131, 187, 337, 1025, 1395, 1671, 1812
SspI	AATATT	2 cut(s)	517, 1410
SspMI	CTAG	4 cut(s)	240, 444, 665, 768
SstI	GAGCTC	1 cut(s)	1240
StyD4I	CCNGG	6 cut(s)	211, 221, 259, 880, 1027, 1831
SwaI	ATTTAAAT	1 cut(s)	513
TaaI	ACNGT	3 cut(s)	326, 703, 837
TaiI	ACGT	5 cut(s)	700, 1064, 1262, 1438, 1494
TaqI	TCGA	2 cut(s)	283, 520
TasI	AATT	8 cut(s)	31, 508, 645, 990, 1105, 1152, 1169, 1471
TatI	WGTACW	1 cut(s)	1142
TauI	GCSGC	2 cut(s)	133, 1815
TfiI	GAWTC	3 cut(s)	20, 46, 657
Tru1I	TTAA	6 cut(s)	408, 512, 1151, 1379, 1563, 1845
Tru9I	TTAA	6 cut(s)	408, 512, 1151, 1379, 1563, 1845
TscAI	CASTG	3 cut(s)	280, 441, 1310
TseFI	GTSAC	1 cut(s)	1346
TseI	GCWGC	2 cut(s)	629, 1430
Tsp45I	GTSAC	1 cut(s)	1346
TspDTI	ATGAA	7 cut(s)	51, 375, 774, 1166, 1205, 1331, 1331
TspRI	CASTG	3 cut(s)	280, 441, 1310
VneI	GTGCAC	1 cut(s)	819
VpaK11BI	GGWCC	3 cut(s)	322, 1207, 1829
XagI	CCTNNNNNAGG	1 cut(s)	1026
XapI	RAATTY	4 cut(s)	31, 508, 1169, 1471
XceI	RCATGY	2 cut(s)	1021, 1376
XmnI	GAANNNNTTC	1 cut(s)	1173
XspI	CTAG	4 cut(s)	240, 444, 665, 768
ZrmI	AGTACT	1 cut(s)	1144
Zsp2I	ATGCAT	3 cut(s)	1297, 1378, 1654

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.