MD15G1175000.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000684
Physical Location & Seq
Reverse (-)
13612197 .. 13613760
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1175000.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCCGGTGCTCAGACCGGGTCCGGCAAGACGGCTGCGTTTTGCTTCCCCATCATCAATGGGGTCTTAAAGAATGGCGTCGAGTGGTCGCCGGCTCGCGCCGGGGATCGTGCTGTGTGCCCTACCGCCCTCATTTTGTCTCCGACGAGAGAGCTGGCTGGTCAGATTCATGAGGAAGCTAAGAAGTTTGGTTATCAGAGTGGGGTGAAGATTGTTGTTGGTTATGGTGGAGCTCCAATTTCTCAGCAGTTGCGTATGCTGGAGAGAGGCTGTGATATTCTTGTTGCAACTCCTGGGCGCTTAGTGGACATGATTGAGAGGTCACGAGTTTCGCTGAGAAATGTCAAATATTTGGCATTAGATGAGGCTGATAGGATGTTGGACATGGGTTTTGAGCCTCAGATAAGGAGGATTGTTGAACAAACGGACATGCCTCGCTCTGGTGCAAGACAAACGTTGCTTTTCAGTGCTACATTTCCAGATGAAATACAGAGGCTTGCTGCAGATTTTCTGTTAAACTACATATTTCTCGCTGTTGGAAGAGTTGGATCAAGCACAGGTCTCATCACACAAAGAGTTGGTTCAATTTTGGTCAATGGAGATTGGAGATTCGAACTTGGTATCTCGTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 ko05161 ko05203 map04622 map05161 map05203
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

212

Amino Acids

23.02

Weight (kDa)

8.88

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 2 - 165 5.9e-44 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AciI CCGC 1 cut(s) 126
AclI AACGTT 1 cut(s) 455
AclWI GGATC 2 cut(s) 114, 556
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 78
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 2 cut(s) 419, 585
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 3 cut(s) 154, 179, 233
AluI AGCT 3 cut(s) 154, 179, 233
Alw21I GWGCWC 2 cut(s) 13, 235
Alw26I GTCTC 2 cut(s) 144, 566
AlwI GGATC 2 cut(s) 114, 556
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 35, 500
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AspLEI GCGC 2 cut(s) 101, 300
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 2 cut(s) 19, 103
AsuHPI GGTGA 1 cut(s) 217
AsuII TTCGAA 1 cut(s) 612
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 235
BauI CACGAG 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 13, 235
BbvI GCAGC 2 cut(s) 22, 487
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 294
BcnI CCSGG 2 cut(s) 19, 103
BcoDI GTCTC 2 cut(s) 144, 566
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 301
BisI GCNGC 2 cut(s) 36, 501
BlsI GCNGC 2 cut(s) 37, 502
Bme1390I CCNGG 3 cut(s) 19, 103, 294
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 3 cut(s) 19, 103, 294
BpmI CTGGAG 1 cut(s) 281
Bpu14I TTCGAA 1 cut(s) 612
BpuMI CCSGG 2 cut(s) 19, 103
BsaHI GRCGYC 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 2 cut(s) 102, 293
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 2 cut(s) 5, 91
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 102, 293
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 4 cut(s) 26, 257, 326, 413
BseSI GKGCMC 1 cut(s) 122
BseXI GCAGC 2 cut(s) 22, 487
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 13, 235
BsiSI CCGG 5 cut(s) 6, 18, 24, 92, 102
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 2 cut(s) 144, 566
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 566
Bsp119I TTCGAA 1 cut(s) 612
Bsp1286I GDGCHC 3 cut(s) 13, 122, 235
Bsp143I GATC 2 cut(s) 106, 548
BspACI CCGC 1 cut(s) 126
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 25, 256, 327, 412
BspFNI CGCG 1 cut(s) 99
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 2 cut(s) 114, 556
BspT104I TTCGAA 1 cut(s) 612
BspTNI GGTCTC 1 cut(s) 566
BsrFI RCCGGY 2 cut(s) 5, 91
BssAI RCCGGY 2 cut(s) 5, 91
BssECI CCNNGG 2 cut(s) 102, 293
BssMI GATC 2 cut(s) 106, 548
BssNI GRCGYC 1 cut(s) 78
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 294
Bst6I CTCTTC 1 cut(s) 535
BstACI GRCGYC 1 cut(s) 78
BstBI TTCGAA 1 cut(s) 612
BstC8I GCNNGC 4 cut(s) 93, 97, 156, 498
BstDEI CTNAG 6 cut(s) 12, 180, 243, 301, 335, 399
BstF5I GGATG 1 cut(s) 381
BstFNI CGCG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 301
BstHHI GCGC 2 cut(s) 101, 300
BstKTI GATC 2 cut(s) 109, 551
BstMAI GTCTC 2 cut(s) 144, 566
BstMBI GATC 2 cut(s) 106, 548
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 433
BstSCI CCNGG 3 cut(s) 17, 101, 292
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 122
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 2 cut(s) 22, 487
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 472
Cac8I GCNNGC 4 cut(s) 93, 97, 156, 498
CciI TCATGA 1 cut(s) 169
CfoI GCGC 2 cut(s) 101, 300
Cfr10I RCCGGY 2 cut(s) 5, 91
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 1 cut(s) 67
CviAII CATG 4 cut(s) 170, 310, 385, 430
DdeI CTNAG 6 cut(s) 12, 180, 243, 301, 335, 399
DpnI GATC 2 cut(s) 108, 550
DpnII GATC 2 cut(s) 106, 548
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 535
EarI CTCTTC 1 cut(s) 535
Ecl136II GAGCTC 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 235
Eco31I GGTCTC 1 cut(s) 566
Eco47I GGWCC 1 cut(s) 21
Eco53kI GAGCTC 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 4 cut(s) 173, 313, 388, 433
FaiI YATR 7 cut(s) 171, 225, 257, 311, 386, 431, 524
FatI CATG 4 cut(s) 169, 309, 384, 429
Fnu4HI GCNGC 2 cut(s) 36, 501
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 36, 501
GlaI GCGC 2 cut(s) 100, 299
GluI GCNGC 2 cut(s) 36, 501
GsuI CTGGAG 1 cut(s) 281
HaeII RGCGCY 1 cut(s) 301
HaeIII GGCC 1 cut(s) 5
HapII CCGG 5 cut(s) 6, 18, 24, 92, 102
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 101, 300
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 4 cut(s) 173, 313, 388, 433
Hin6I GCGC 2 cut(s) 99, 298
HinP1I GCGC 2 cut(s) 99, 298
HinfI GANTC 2 cut(s) 166, 609
HpaII CCGG 5 cut(s) 6, 18, 24, 92, 102
HphI GGTGA 1 cut(s) 217
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 5 cut(s) 15, 144, 165, 198, 402
Hpy188III TCNNGA 3 cut(s) 170, 324, 479
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 2 cut(s) 83, 148
HpyCH4IV ACGT 1 cut(s) 455
HpyCH4V TGCA 3 cut(s) 287, 446, 503
HpyF3I CTNAG 6 cut(s) 12, 180, 243, 301, 335, 399
HpySE526I ACGT 1 cut(s) 455
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 4 cut(s) 173, 313, 388, 433
HspAI GCGC 2 cut(s) 99, 298
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Kzo9I GATC 2 cut(s) 106, 548
LmnI GCTCC 2 cut(s) 230, 238
Lsp1109I GCAGC 2 cut(s) 22, 487
MaeII ACGT 1 cut(s) 455
MaeIII GTNAC 1 cut(s) 321
MalI GATC 2 cut(s) 108, 550
MboI GATC 2 cut(s) 106, 548
MboII GAAGA 2 cut(s) 220, 552
MhlI GDGCHC 3 cut(s) 13, 122, 235
MluCI AATT 2 cut(s) 237, 585
MmeI TCCRAC 4 cut(s) 167, 360, 517, 526
MnlI CCTC 9 cut(s) 140, 166, 260, 312, 358, 402, 408, 444, 486
MroNI GCCGGC 1 cut(s) 91
MseI TTAA 2 cut(s) 68, 515
MspI CCGG 5 cut(s) 6, 18, 24, 92, 102
MspR9I CCNGG 3 cut(s) 19, 103, 294
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 1 cut(s) 99
NaeI GCCGGC 1 cut(s) 93
NciI CCSGG 2 cut(s) 19, 103
NdeII GATC 2 cut(s) 106, 548
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 4 cut(s) 173, 313, 388, 433
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 321
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 612
PagI TCATGA 1 cut(s) 169
PdiI GCCGGC 1 cut(s) 93
PfeI GAWTC 2 cut(s) 166, 609
PflFI GACNNNGTC 1 cut(s) 19
PkrI GCNGC 2 cut(s) 37, 502
Psp124BI GAGCTC 1 cut(s) 235
Psp1406I AACGTT 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 21
PstI CTGCAG 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 19
SacI GAGCTC 1 cut(s) 235
SaqAI TTAA 2 cut(s) 68, 515
SatI GCNGC 2 cut(s) 36, 501
Sau3AI GATC 2 cut(s) 106, 548
Sau96I GGNCC 1 cut(s) 21
ScrFI CCNGG 3 cut(s) 19, 103, 294
SduI GDGCHC 3 cut(s) 13, 122, 235
SetI ASST 6 cut(s) 156, 181, 235, 323, 458, 562
SfcI CTRYAG 1 cut(s) 501
SfuI TTCGAA 1 cut(s) 612
SinI GGWCC 1 cut(s) 21
Sse9I AATT 2 cut(s) 237, 585
SsiI CCGC 1 cut(s) 126
SspI AATATT 1 cut(s) 350
SstI GAGCTC 1 cut(s) 235
StyD4I CCNGG 3 cut(s) 17, 101, 292
TaiI ACGT 1 cut(s) 458
TaqI TCGA 2 cut(s) 81, 612
TasI AATT 2 cut(s) 237, 585
TfiI GAWTC 2 cut(s) 166, 609
Tru1I TTAA 2 cut(s) 68, 515
Tru9I TTAA 2 cut(s) 68, 515
TscAI CASTG 1 cut(s) 472
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 35, 500
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 2 cut(s) 158, 498
TspGWI ACGGA 1 cut(s) 440
TspRI CASTG 1 cut(s) 472
Tth111I GACNNNGTC 1 cut(s) 19
VpaK11BI GGWCC 1 cut(s) 21
XceI RCATGY 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.