Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0000684

Physical Location & Seq

Genomic Coordinates Reverse (-)

3290237 .. 3294065

Gene Structure 3829 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG041300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1980 bp

ATGAGCCTGAGGACGATGGCTTGGGCCGAAGCGGTGACCGAGTCCGCCCGGAAGCAAACTCGCCCACTTCCCCCGCCGCGCAACACTACCTACTTGCCTCCGCATCTTCGCTACTCCGGCGGCGGCGGCGGCGATGGTGGTGGTTACAGCTCCTACGGCCGTGGCTCTAGTGAGTCGGCTCAGTACGGTTTTGGTGGTGGTGGTTACAGGGGAGGCGGCCGGAGCGGTCCGGGTCGCGACGGAGGACGAGGAGGTCGTCGTGGTGGTGGTGGTGGCCGGAGTCGAGGTTGGGTGCAGACGCCGAGGCCGAACAGGGACGCGTTTCATGACGTGTCCGGGAAATTTGAGCAGCTGAAAGTGAGGGAGGGGGATGACGTCGGCAATGTGGGTATAAATTTCGATGCTTACGAGGATATTCCGGTGGAGGCGAGCGGCGAGGACGTGCCGGCGCCGGCGAGCACTTTTGGGGAGATTGATTTGGGGGAGTGCTTGAACAACAACATCAAGAGGTGTAGGTACGTGAAGCCGACGCCGGTTCAGCGCCACGCGATTCCGATAGCCATGGCCGGCCGGGACTTGATGGCTTGTGCTCAGACCGGGTCCGGAAAGACGGCGGCTTTTTGCTTCCCCATTATCAGTGGAGTGTTGACTAAGTGTGTGGAGAGGTCTCAGAGCAATGGGGATGGTTGGACTGTGTTCCCCAGAGCTCTCATTTTGTCTCCGACGAGAGAGTTGGCTAGTCAGATATTTGAGGAAGCTGAGAAGTTTGCTTATCAGAGTGGGGCAAAAGTGGCTGTTGTTTATGGTGGAGCGCCTATGGGTCAGCAGCTTCGTGATCTGGAGAGAGGTGTTGACATCCTAGTCGCCACTCCAGGGCGCTTAGTGGACATGATTGAAAGGTCACGAGTTTCACTCAAGATGATCAAATACTTGGCACTAGATGAGGCTGATAGGATGTTGGACATGGGTTTTGAACCTCAAATAAGGAAGATTGTTCAAAGAATGGACATGCCTCCCCCAGGTGCAAGACAAACACTGCTTTTCAGTGCAACATTCCCGCAAGAAATACAGAGGCTTGCTGCAGATTTCCTCAAGAACTACATTTTTCTGACTGTTGGGAGAGTTGGGTCAAGCACTGATCTTATAGCACAAAAGGTTGAGTTTGTCAGGGATATGGACAAAATACAGCATTTATGTGGTCTTCTTAAGAACCAGAGGGACAATGAAACTCATGGAAAGCTTGCTTTAACCTTGGTGTTTGTTGAGACCAAGAAAGGAGCAAATCAATTGGAAAACTGGTTGTCCAGGAATGGATTCCCAGCAATAGCAATACATGGTGACAAAGTGCAAATGGAGAGGGAACGAGCTTTGAGATTGTTCAAGTGTGGCACAACTCCAATACTGGTGGCAACAGATGTTGCTTCACGAGGTTTAGATATCCCTCATGTTGCCCATGTCATCAATTTTGACTTCCCAAAAAGCATAGATGATTATGTTCACCGGATTGGTCGGACTGGACGTGCTGGTAAATCTGGTCTTGCAACTGCTTTCTTCAGTGAAAAGAACCAGCCTCTTGCAAAGGCACTAGTGGAGTTAATGAAGGAGTCAAAGCAGGAGGTTCCTGATTGGCTTAATGATTATGCTGAACAGTCATTGTCTTATGGTGGAGACCAGAGCCAAAGGTACAGTAGCAGTAACTTTGGTGGATATGACGTCCGTAGGAGTGAGAATTATTATGGTTCAGGCAGTTATGGTTATGGAGATTCTTCTGTGGGTGCGGCTGCTGCTACTTGTAATGATGTTTCTGGTTCATATGCTACTGGTTCATTTGGCCATGGAGGAAATTATGGGAATTCTTCTGTGGATGAGCCTGCTGCTTCCTACAATGGTTCTTATGGGGGTGAAACTGATGCTGCTGGTACTGCTGGTTCATCGAGCTTTGGAAATTATGAAAATATTGTTGCTGGTGGGTGGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04622 ko05161 ko05203 map04622 map05161 map05203

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

659

Amino Acids

71.07

Weight (kDa)

7.58

Isoelectric Point (pI)

39.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	177 - 358	1.7e-50	DEAD/DEAH box helicase
Helicase_C	PF00271	394 - 509	1.5e-29	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42520 AT2G42520 AT2G42520 AT3G58510 AT3G58510 AT3G58510 AT3G58570
fragaria_vesca	FvH4_1g03520 FvH4_1g03520 FvH4_1g03520 FvH4_3g45200 FvH4_3g45200 FvH4_3g45200 FvH4_6g21570
malus_domestica	MD02G1034300.v1.1 MD03G1007400.v1.1 MD11G1010700.v1.1 MD12G1053000.v1.1 MD14G1051400.v1.1 MD15G1175000.v1.1
prunus_persica	Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.7G084600_v2.0.a1 Prupe.7G240900_v2.0.a1
pyrus_communis	pycom02g02800 pycom03g00760 pycom11g00670 pycom12g04630 pycom14g04260 pycom15g15650
rosa_chinensis	RchiOBHm_Chr2g0088731 RchiOBHm_Chr2g0088781 RchiOBHm_Chr2g0088941 RchiOBHm_Chr3g0478811 RchiOBHm_Chr5g0082811
rosa_laevigata	RLG00000015980 RLG00000016349 RLG00000023623 RLG00000036991
rosa_multiflora	Rmu_co8138978.1_g000001 Rmu_co8475903.1_g000001 Rmu_sc0000965.1_g000010 Rmu_sc0001381.1_g000020 Rmu_sc0001519.1_g000001 Rmu_sc0005751.1_g000004 Rmu_sc0011683.1_g000004
rosa_roxburghii	Rroxscaffold_1G00001630 Rroxscaffold_2G00148710 Rroxscaffold_6G00403050
rosa_rugosa	Rorug01G0486600 Rorug03G0168400 Rorug05G0479900 Rorug05G0479900 Rorug05G0479900 Rorug05G0480000
rosa_samantha	Rh2AG041800 Rh2AG076200 Rh2BG040500 Rh2BG077600 Rh2CG041300 Rh2CG041900 Rh2CG079800 Rh2DG040900 Rh2DG074900 Rh3AG220200 Rh3BG252000 Rh3DG245100 Rh5AG530300 Rh5DG565600
rosa_wichuraiana	Rw2G003260 Rw2G006990 Rw3G019790 Rw5G049570

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	252, 860
AatII	GACGTC	2 cut(s)	378, 1716
AccB1I	GGYRCC	1 cut(s)	448
AccBSI	CCGCTC	2 cut(s)	225, 432
AccII	CGCG	4 cut(s)	79, 237, 320, 548
AccIII	TCCGGA	1 cut(s)	602
AcoI	YGGCCR	6 cut(s)	157, 217, 274, 564, 568, 1831
AcsI	RAATTY	3 cut(s)	341, 394, 1852
AcuI	CTGAAG	1 cut(s)	1537
AcyI	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
AfaI	GTAC	4 cut(s)	185, 518, 1685, 1921
AfiI	CCNNNNNNNGG	2 cut(s)	873, 1019
AflII	CTTAAG	1 cut(s)	1205
AflIII	ACRYGT	2 cut(s)	318, 330
AgsI	TTSAA	5 cut(s)	493, 896, 974, 998, 1381
AhlI	ACTAGT	1 cut(s)	1585
AjiI	CACGTC	3 cut(s)	331, 442, 1520
AjnI	CCWGG	3 cut(s)	871, 1018, 1304
AluBI	AGCT	8 cut(s)	150, 352, 707, 758, 829, 1240, 1367, 1938
AluI	AGCT	8 cut(s)	150, 352, 707, 758, 829, 1240, 1367, 1938
Alw21I	GWGCWC	3 cut(s)	461, 592, 709
Alw26I	GTCTC	4 cut(s)	672, 723, 1259, 1662
Aor13HI	TCCGGA	1 cut(s)	602
AoxI	GGCC	8 cut(s)	24, 157, 217, 274, 305, 564, 568, 1831
ApeKI	GCWGC	7 cut(s)	349, 826, 1079, 1781, 1784, 1874, 1913
ApoI	RAATTY	3 cut(s)	341, 394, 1852
ArsI	GACNNNNNNTTYG	2 cut(s)	953, 985
Asp700I	GAANNNNTTC	1 cut(s)	1313
AspLEI	GCGC	5 cut(s)	81, 451, 543, 814, 879
AspS9I	GGNCC	3 cut(s)	24, 227, 600
AsuC2I	CCSGG	5 cut(s)	49, 231, 337, 572, 598
AsuHPI	GGTGA	4 cut(s)	46, 1349, 1490, 1913
AvaII	GGWCC	2 cut(s)	227, 600
AxyI	CCTNAGG	1 cut(s)	8
BalI	TGGCCA	1 cut(s)	1833
BanI	GGYRCC	1 cut(s)	448
BanII	GRGCYC	1 cut(s)	709
BauI	CACGAG	2 cut(s)	903, 1425
BbsI	GAAGAC	1 cut(s)	1193
Bbv12I	GWGCWC	3 cut(s)	461, 592, 709
BbvI	GCAGC	7 cut(s)	361, 838, 1066, 1768, 1771, 1861, 1900
BccI	CCATC	4 cut(s)	10, 128, 574, 677
BceAI	ACGGC	3 cut(s)	144, 172, 627
BciT130I	CCWGG	3 cut(s)	873, 1020, 1306
BclI	TGATCA	1 cut(s)	921
BcnI	CCSGG	5 cut(s)	49, 231, 337, 572, 598
BcoDI	GTCTC	4 cut(s)	672, 723, 1259, 1662
BcuI	ACTAGT	1 cut(s)	1585
BfaI	CTAG	5 cut(s)	168, 738, 860, 938, 1586
BfmI	CTRYAG	1 cut(s)	1080
BfoI	RGCGCY	4 cut(s)	452, 544, 815, 880
BfrI	CTTAAG	1 cut(s)	1205
Bme1390I	CCNGG	8 cut(s)	49, 231, 337, 572, 598, 873, 1020, 1306
Bme18I	GGWCC	2 cut(s)	227, 600
BmgBI	CACGTC	3 cut(s)	331, 442, 1520
BmgT120I	GGNCC	3 cut(s)	24, 227, 600
BmiI	GGNNCC	3 cut(s)	450, 601, 1620
BmrFI	CCNGG	8 cut(s)	49, 231, 337, 572, 598, 873, 1020, 1306
BmsI	GCATC	3 cut(s)	112, 391, 1900
BpiI	GAAGAC	1 cut(s)	1193
BplI	GAGNNNNNCTC	2 cut(s)	897, 929
BpmI	CTGGAG	2 cut(s)	855, 860
BpuEI	CTTGAG	2 cut(s)	899, 1076
BpuMI	CCSGG	5 cut(s)	49, 231, 337, 572, 598
BsaAI	YACGTR	1 cut(s)	520
BsaHI	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
BsaI	GGTCTC	3 cut(s)	672, 1259, 1662
BsaJI	CCNNGG	7 cut(s)	160, 302, 561, 872, 1018, 1251, 1834
BsaWI	WCCGGW	3 cut(s)	418, 602, 1500
BsaXI	ACNNNNNCTCC	2 cut(s)	271, 301
Bsc4I	CCNNNNNNNGG	2 cut(s)	873, 1019
Bse118I	RCCGGY	4 cut(s)	445, 451, 532, 566
Bse1I	ACTGG	4 cut(s)	1301, 1407, 1519, 1825
Bse21I	CCTNAGG	1 cut(s)	8
Bse3DI	GCAATG	2 cut(s)	388, 682
BseAI	TCCGGA	1 cut(s)	602
BseBI	CCWGG	3 cut(s)	873, 1020, 1306
BseDI	CCNNGG	7 cut(s)	160, 302, 561, 872, 1018, 1251, 1834
BseGI	GGATG	5 cut(s)	376, 688, 855, 960, 1870
BseLI	CCNNNNNNNGG	2 cut(s)	873, 1019
BseMI	GCAATG	2 cut(s)	388, 682
BseMII	CTCAG	4 cut(s)	194, 605, 683, 750
BseNI	ACTGG	4 cut(s)	1301, 1407, 1519, 1825
BseRI	GAGGAG	1 cut(s)	264
BseX3I	CGGCCG	3 cut(s)	157, 217, 568
BseXI	GCAGC	7 cut(s)	361, 838, 1066, 1768, 1771, 1861, 1900
BseYI	CCCAGC	1 cut(s)	1318
BsgI	GTGCAG	1 cut(s)	314
Bsh1236I	CGCG	4 cut(s)	79, 237, 320, 548
Bsh1285I	CGRYCG	3 cut(s)	160, 220, 571
BshFI	GGCC	8 cut(s)	26, 159, 219, 276, 307, 566, 570, 1833
BshNI	GGYRCC	1 cut(s)	448
BsiEI	CGRYCG	3 cut(s)	160, 220, 571
BsiHKAI	GWGCWC	3 cut(s)	461, 592, 709
BslFI	GGGAC	3 cut(s)	329, 587, 1232
BslI	CCNNNNNNNGG	2 cut(s)	873, 1019
BsmAI	GTCTC	4 cut(s)	672, 723, 1259, 1662
BsmFI	GGGAC	3 cut(s)	329, 587, 1232
BsnI	GGCC	8 cut(s)	26, 159, 219, 276, 307, 566, 570, 1833
Bso31I	GGTCTC	3 cut(s)	672, 1259, 1662
Bsp1286I	GDGCHC	3 cut(s)	461, 592, 709
Bsp13I	TCCGGA	1 cut(s)	602
Bsp143I	GATC	3 cut(s)	835, 921, 1138
Bsp19I	CCATGG	2 cut(s)	561, 1834
Bsp68I	TCGCGA	1 cut(s)	237
BspANI	GGCC	8 cut(s)	26, 159, 219, 276, 307, 566, 570, 1833
BspCNI	CTCAG	4 cut(s)	193, 604, 682, 751
BspEI	TCCGGA	1 cut(s)	602
BspFNI	CGCG	4 cut(s)	79, 237, 320, 548
BspHI	TCATGA	1 cut(s)	325
BspLI	GGNNCC	3 cut(s)	450, 601, 1620
BspMAI	CTGCAG	1 cut(s)	1084
BspT107I	GGYRCC	1 cut(s)	448
BspTI	CTTAAG	1 cut(s)	1205
BspTNI	GGTCTC	3 cut(s)	672, 1259, 1662
BsrBI	CCGCTC	2 cut(s)	225, 432
BsrDI	GCAATG	2 cut(s)	388, 682
BsrFI	RCCGGY	4 cut(s)	445, 451, 532, 566
BsrI	ACTGG	4 cut(s)	1301, 1407, 1519, 1825
BssAI	RCCGGY	4 cut(s)	445, 451, 532, 566
BssECI	CCNNGG	7 cut(s)	160, 302, 561, 872, 1018, 1251, 1834
BssMI	GATC	3 cut(s)	835, 921, 1138
BssNI	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
BssSI	CACGAG	2 cut(s)	903, 1425
BssT1I	CCWWGG	3 cut(s)	561, 1251, 1834
Bst2BI	CACGAG	2 cut(s)	903, 1425
Bst2UI	CCWGG	3 cut(s)	873, 1020, 1306
Bst4CI	ACNGT	5 cut(s)	188, 694, 1114, 1650, 1688
BstACI	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
BstAFI	CTTAAG	1 cut(s)	1205
BstBAI	YACGTR	1 cut(s)	520
BstC8I	GCNNGC	8 cut(s)	430, 447, 453, 457, 568, 1077, 1242, 1872
BstDEI	CTNAG	7 cut(s)	8, 180, 591, 651, 669, 759, 880
BstDSI	CCRYGG	3 cut(s)	160, 561, 1834
BstEII	GGTNACC	1 cut(s)	34
BstENI	CCTNNNNNAGG	1 cut(s)	1017
BstF5I	GGATG	5 cut(s)	376, 688, 855, 960, 1870
BstFNI	CGCG	4 cut(s)	79, 237, 320, 548
BstH2I	RGCGCY	4 cut(s)	452, 544, 815, 880
BstHHI	GCGC	5 cut(s)	81, 451, 543, 814, 879
BstKTI	GATC	3 cut(s)	838, 924, 1141
BstMAI	GTCTC	4 cut(s)	672, 723, 1259, 1662
BstMBI	GATC	3 cut(s)	835, 921, 1138
BstMCI	CGRYCG	3 cut(s)	160, 220, 571
BstMWI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 222, 538, 791, 1784, 1922
BstNI	CCWGG	3 cut(s)	873, 1020, 1306
BstNSI	RCATGY	1 cut(s)	1012
BstPI	GGTNACC	1 cut(s)	34
BstSCI	CCNGG	8 cut(s)	47, 229, 335, 570, 596, 871, 1018, 1304
BstSFI	CTRYAG	1 cut(s)	1080
BstUI	CGCG	4 cut(s)	79, 237, 320, 548
BstV1I	GCAGC	7 cut(s)	361, 838, 1066, 1768, 1771, 1861, 1900
BstV2I	GAAGAC	1 cut(s)	1193
BstZI	CGGCCG	3 cut(s)	157, 217, 568
Bsu36I	CCTNAGG	1 cut(s)	8
BsuRI	GGCC	8 cut(s)	26, 159, 219, 276, 307, 566, 570, 1833
BtgI	CCRYGG	3 cut(s)	160, 561, 1834
BtgZI	GCGATG	1 cut(s)	147
BtrI	CACGTC	3 cut(s)	331, 442, 1520
BtsCI	GGATG	5 cut(s)	376, 688, 855, 960, 1870
BtsI	GCAGTG	1 cut(s)	1034
BtsIMutI	CAGTG	5 cut(s)	643, 1034, 1051, 1134, 1561
BtuMI	TCGCGA	1 cut(s)	237
Cac8I	GCNNGC	8 cut(s)	430, 447, 453, 457, 568, 1077, 1242, 1872
CciI	TCATGA	1 cut(s)	325
CfoI	GCGC	5 cut(s)	81, 451, 543, 814, 879
Cfr10I	RCCGGY	4 cut(s)	445, 451, 532, 566
Cfr13I	GGNCC	3 cut(s)	24, 227, 600
CpoI	CGGWCCG	1 cut(s)	227
CseI	GACGC	3 cut(s)	307, 326, 538
Csp6I	GTAC	4 cut(s)	184, 517, 1684, 1920
CspCI	CAANNNNNGTGG	2 cut(s)	1386, 1421
CspI	CGGWCCG	1 cut(s)	227
CviQI	GTAC	4 cut(s)	184, 517, 1684, 1920
DdeI	CTNAG	7 cut(s)	8, 180, 591, 651, 669, 759, 880
DinI	GGCGCC	1 cut(s)	450
DpnI	GATC	3 cut(s)	837, 923, 1140
DpnII	GATC	3 cut(s)	835, 921, 1138
DrdI	GACNNNNNNGTC	2 cut(s)	252, 860
DseDI	GACNNNNNNGTC	2 cut(s)	252, 860
EaeI	YGGCCR	6 cut(s)	157, 217, 274, 564, 568, 1831
EagI	CGGCCG	3 cut(s)	157, 217, 568
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	707
EclXI	CGGCCG	3 cut(s)	157, 217, 568
Eco130I	CCWWGG	3 cut(s)	561, 1251, 1834
Eco24I	GRGCYC	1 cut(s)	709
Eco31I	GGTCTC	3 cut(s)	672, 1259, 1662
Eco32I	GATATC	1 cut(s)	1438
Eco47I	GGWCC	2 cut(s)	227, 600
Eco52I	CGGCCG	3 cut(s)	157, 217, 568
Eco53kI	GAGCTC	1 cut(s)	707
Eco57I	CTGAAG	1 cut(s)	1537
Eco81I	CCTNAGG	1 cut(s)	8
Eco91I	GGTNACC	1 cut(s)	34
EcoICRI	GAGCTC	1 cut(s)	707
EcoNI	CCTNNNNNAGG	1 cut(s)	1017
EcoO65I	GGTNACC	1 cut(s)	34
EcoRI	GAATTC	1 cut(s)	1852
EcoRII	CCWGG	3 cut(s)	871, 1018, 1304
EcoRV	GATATC	1 cut(s)	1438
EcoT14I	CCWWGG	3 cut(s)	561, 1251, 1834
EcoT38I	GRGCYC	1 cut(s)	709
EgeI	GGCGCC	1 cut(s)	450
EheI	GGCGCC	1 cut(s)	450
ErhI	CCWWGG	3 cut(s)	561, 1251, 1834
FaqI	GGGAC	3 cut(s)	329, 587, 1232
FauI	CCCGC	2 cut(s)	81, 1065
FauNDI	CATATG	1 cut(s)	1813
FbaI	TGATCA	1 cut(s)	921
FokI	GGATG	5 cut(s)	383, 695, 842, 967, 1877
FriOI	GRGCYC	1 cut(s)	709
FseI	GGCCGGCC	1 cut(s)	570
FspBI	CTAG	5 cut(s)	168, 738, 860, 938, 1586
GlaI	GCGC	5 cut(s)	80, 450, 542, 813, 878
GsaI	CCCAGC	1 cut(s)	1322
GsuI	CTGGAG	2 cut(s)	855, 860
HaeII	RGCGCY	4 cut(s)	452, 544, 815, 880
HaeIII	GGCC	8 cut(s)	26, 159, 219, 276, 307, 566, 570, 1833
HgaI	GACGC	3 cut(s)	307, 326, 538
HhaI	GCGC	5 cut(s)	81, 451, 543, 814, 879
Hin1I	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
Hin6I	GCGC	5 cut(s)	79, 449, 541, 812, 877
HinP1I	GCGC	5 cut(s)	79, 449, 541, 812, 877
HincII	GTYRAC	2 cut(s)	648, 853
HindII	GTYRAC	2 cut(s)	648, 853
HindIII	AAGCTT	1 cut(s)	1238
HinfI	GANTC	7 cut(s)	41, 173, 280, 550, 1314, 1604, 1763
HphI	GGTGA	4 cut(s)	46, 1349, 1490, 1913
Hpy166II	GTNNAC	4 cut(s)	648, 853, 886, 1498
Hpy188I	TCNGA	8 cut(s)	555, 594, 672, 723, 744, 777, 1110, 1512
Hpy8I	GTNNAC	4 cut(s)	648, 853, 886, 1498
Hpy99I	CGWCG	5 cut(s)	242, 261, 380, 532, 727
HpyAV	CCTTC	1 cut(s)	1594
HpyCH4III	ACNGT	5 cut(s)	188, 694, 1114, 1650, 1688
HpyCH4IV	ACGT	6 cut(s)	330, 375, 441, 519, 1519, 1713
HpyCH4V	TGCA	7 cut(s)	295, 1025, 1049, 1082, 1348, 1541, 1577
HpyF10VI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 222, 538, 791, 1784, 1922
HpyF3I	CTNAG	7 cut(s)	8, 180, 591, 651, 669, 759, 880
HpySE526I	ACGT	6 cut(s)	330, 375, 441, 519, 1519, 1713
Hsp92I	GRCGYC	5 cut(s)	299, 375, 449, 530, 1713
HspAI	GCGC	5 cut(s)	79, 449, 541, 812, 877
KasI	GGCGCC	1 cut(s)	448
Kpn2I	TCCGGA	1 cut(s)	602
KroI	GCCGGC	3 cut(s)	445, 451, 566
KroNI	GCCGGC	3 cut(s)	447, 453, 568
Ksp22I	TGATCA	1 cut(s)	921
Kzo9I	GATC	3 cut(s)	835, 921, 1138
LmnI	GCTCC	4 cut(s)	155, 222, 809, 1277
Lsp1109I	GCAGC	7 cut(s)	361, 838, 1066, 1768, 1771, 1861, 1900
LweI	GCATC	3 cut(s)	112, 391, 1900
MaeI	CTAG	5 cut(s)	168, 738, 860, 938, 1586
MaeII	ACGT	6 cut(s)	330, 375, 441, 519, 1519, 1713
MaeIII	GTNAC	6 cut(s)	34, 143, 203, 900, 1337, 1694
MalI	GATC	3 cut(s)	837, 923, 1140
MbiI	CCGCTC	2 cut(s)	225, 432
MboI	GATC	3 cut(s)	835, 921, 1138
MboII	GAAGA	6 cut(s)	98, 1000, 1193, 1543, 1758, 1848
MfeI	CAATTG	1 cut(s)	1286
MhlI	GDGCHC	3 cut(s)	461, 592, 709
MlsI	TGGCCA	1 cut(s)	1833
MluCI	AATT	8 cut(s)	341, 394, 1286, 1462, 1729, 1843, 1852, 1945
MluI	ACGCGT	1 cut(s)	318
MluNI	TGGCCA	1 cut(s)	1833
Mly113I	GGCGCC	1 cut(s)	449
MlyI	GAGTC	4 cut(s)	50, 182, 289, 1613
MmeI	TCCRAC	4 cut(s)	668, 746, 939, 1490
Mox20I	TGGCCA	1 cut(s)	1833
MreI	CGCCGGCG	1 cut(s)	451
MroI	TCCGGA	1 cut(s)	602
MroNI	GCCGGC	3 cut(s)	445, 451, 566
MroXI	GAANNNNTTC	1 cut(s)	1313
MscI	TGGCCA	1 cut(s)	1833
MseI	TTAA	4 cut(s)	1206, 1247, 1595, 1632
MslI	CAYNNNNRTG	1 cut(s)	1194
Msp20I	TGGCCA	1 cut(s)	1833
MspA1I	CMGCKG	1 cut(s)	352
MspCI	CTTAAG	1 cut(s)	1205
MspR9I	CCNGG	8 cut(s)	49, 231, 337, 572, 598, 873, 1020, 1306
MunI	CAATTG	1 cut(s)	1286
MvaI	CCWGG	3 cut(s)	873, 1020, 1306
MvnI	CGCG	4 cut(s)	79, 237, 320, 548
MwoI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 222, 538, 791, 1784, 1922
NaeI	GCCGGC	3 cut(s)	447, 453, 568
NarI	GGCGCC	1 cut(s)	449
NciI	CCSGG	5 cut(s)	49, 231, 337, 572, 598
NcoI	CCATGG	2 cut(s)	561, 1834
NdeI	CATATG	1 cut(s)	1813
NdeII	GATC	3 cut(s)	835, 921, 1138
NgoMIV	GCCGGC	3 cut(s)	445, 451, 566
NlaIV	GGNNCC	3 cut(s)	450, 601, 1620
NmeAIII	GCCGAG	1 cut(s)	327
NmuCI	GTSAC	3 cut(s)	34, 900, 1337
NruI	TCGCGA	1 cut(s)	237
NspI	RCATGY	1 cut(s)	1012
PagI	TCATGA	1 cut(s)	325
PcsI	WCGNNNNNNNCGW	4 cut(s)	253, 305, 405, 1516
PdiI	GCCGGC	3 cut(s)	447, 453, 568
PdmI	GAANNNNTTC	1 cut(s)	1313
PfeI	GAWTC	3 cut(s)	550, 1314, 1763
PflFI	GACNNNGTC	2 cut(s)	40, 598
PfoI	TCCNGGA	2 cut(s)	335, 1304
PleI	GAGTC	4 cut(s)	49, 181, 288, 1612
PluTI	GGCGCC	1 cut(s)	452
PpsI	GAGTC	4 cut(s)	49, 181, 288, 1612
Ppu21I	YACGTR	1 cut(s)	520
Psp124BI	GAGCTC	1 cut(s)	709
Psp6I	CCWGG	3 cut(s)	871, 1018, 1304
PspEI	GGTNACC	1 cut(s)	34
PspFI	CCCAGC	1 cut(s)	1318
PspGI	CCWGG	3 cut(s)	871, 1018, 1304
PspN4I	GGNNCC	3 cut(s)	450, 601, 1620
PspPI	GGNCC	3 cut(s)	24, 227, 600
PsrI	GAACNNNNNNTAC	2 cut(s)	1912, 1944
PstI	CTGCAG	1 cut(s)	1084
PsyI	GACNNNGTC	2 cut(s)	40, 598
PvuII	CAGCTG	1 cut(s)	352
RigI	GGCCGGCC	1 cut(s)	570
RruI	TCGCGA	1 cut(s)	237
RsaI	GTAC	4 cut(s)	185, 518, 1685, 1921
RsaNI	GTAC	4 cut(s)	184, 517, 1684, 1920
RseI	CAYNNNNRTG	1 cut(s)	1194
Rsr2I	CGGWCCG	1 cut(s)	227
RsrII	CGGWCCG	1 cut(s)	227
SacI	GAGCTC	1 cut(s)	709
SaqAI	TTAA	4 cut(s)	1206, 1247, 1595, 1632
Sau3AI	GATC	3 cut(s)	835, 921, 1138
Sau96I	GGNCC	3 cut(s)	24, 227, 600
SchI	GAGTC	4 cut(s)	50, 182, 289, 1613
ScrFI	CCNGG	8 cut(s)	49, 231, 337, 572, 598, 873, 1020, 1306
SduI	GDGCHC	3 cut(s)	461, 592, 709
SfaNI	GCATC	3 cut(s)	112, 391, 1900
SfcI	CTRYAG	1 cut(s)	1080
SfoI	GGCGCC	1 cut(s)	450
SgrAI	CRCCGGYG	1 cut(s)	451
SinI	GGWCC	2 cut(s)	227, 600
SmiMI	CAYNNNNRTG	1 cut(s)	1194
SmlI	CTYRAG	3 cut(s)	914, 1091, 1205
SmoI	CTYRAG	3 cut(s)	914, 1091, 1205
SpeI	ACTAGT	1 cut(s)	1585
Sse9I	AATT	8 cut(s)	341, 394, 1286, 1462, 1729, 1843, 1852, 1945
SspDI	GGCGCC	1 cut(s)	448
SspI	AATATT	1 cut(s)	1957
SspMI	CTAG	5 cut(s)	168, 738, 860, 938, 1586
SstI	GAGCTC	1 cut(s)	709
StyD4I	CCNGG	8 cut(s)	47, 229, 335, 570, 596, 871, 1018, 1304
StyI	CCWWGG	3 cut(s)	561, 1251, 1834
TaaI	ACNGT	5 cut(s)	188, 694, 1114, 1650, 1688
TaiI	ACGT	6 cut(s)	333, 378, 444, 522, 1522, 1716
TaqI	TCGA	3 cut(s)	283, 399, 1934
TaqII	GACCGA	1 cut(s)	53
TasI	AATT	8 cut(s)	341, 394, 1286, 1462, 1729, 1843, 1852, 1945
TauI	GCSGC	9 cut(s)	79, 123, 126, 129, 132, 219, 435, 617, 1781
TfiI	GAWTC	3 cut(s)	550, 1314, 1763
Tru1I	TTAA	4 cut(s)	1206, 1247, 1595, 1632
Tru9I	TTAA	4 cut(s)	1206, 1247, 1595, 1632
TscAI	CASTG	5 cut(s)	643, 1041, 1051, 1141, 1561
TseFI	GTSAC	3 cut(s)	34, 900, 1337
TseI	GCWGC	7 cut(s)	349, 826, 1079, 1781, 1784, 1874, 1913
Tsp45I	GTSAC	3 cut(s)	34, 900, 1337
TspDTI	ATGAA	7 cut(s)	314, 1239, 1613, 1800, 1815, 1920, 1965
TspGWI	ACGGA	2 cut(s)	255, 1706
TspRI	CASTG	5 cut(s)	643, 1041, 1051, 1141, 1561
Tth111I	GACNNNGTC	2 cut(s)	40, 598
Vha464I	CTTAAG	1 cut(s)	1205
VpaK11BI	GGWCC	2 cut(s)	227, 600
XagI	CCTNNNNNAGG	1 cut(s)	1017
XapI	RAATTY	3 cut(s)	341, 394, 1852
XceI	RCATGY	1 cut(s)	1012
XmnI	GAANNNNTTC	1 cut(s)	1313
XspI	CTAG	5 cut(s)	168, 738, 860, 938, 1586
ZraI	GACGTC	2 cut(s)	376, 1714

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.