Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0000684

Physical Location & Seq

Genomic Coordinates Reverse (-)

2853778 .. 2857331

Gene Structure 3554 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG040900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGAGCCTGAGGACGATGGCTTGGGCCGAAGCGGTGACCGAGTCCGCCCGGAAGCAAACTCGCCCACTCCCCCCGCCGCGCAACACTACCTACTTGCCTCCGCATCTTCGCTACTCCGGCGGCGGCGGCGGCGATGGTGGTGGTTACAGCTCCTACGGCCGTGGCTCTAGTGAGTCGACTCAGTACGGTTTTGGAGGTGGTGGTGGTGGTGGTTACAGGGGAGGCGGCCGGAGCGGTCCGGGTCGCGACGGAGGACGAGGAGGTCGTGGTGGTGGTGGTGGCCGGAGTCGAGGTTGGGTGCAGACGCCGAGGCCGAACAGGGAGGCGTTTCATGACGTGTCCGGGAAATTTGAGCAGCTGAAAGTGAGGGAGGGGGATGACGTCGGCAATGTGGGTATAAATTTCGATGCTTACGAGGATATTCCGGTGGAGGCGAGCGGCGAGGACGTGCCGGCGCCGGCGAGCACTTTTGGGGAGATTGATTTGGGGGAGTGCTTGAACAACAACATCAAGAGGTGTAGGTACGTGAAGCCGACGCCGGTTCAGCGCCACGCGATTCCGATAGCCATGGCCGGCCGGGACTTGATGGCTTGTGCTCAGACCGGGTCCGGAAAGACGGCGGCTTTTTGCTTCCCCATTATCAGTGGAGTGTTGACTAAGTGTGTGGAGGGGTCTCAGAGTAATGGGGATGGTTGGACTGTGTTTCCCAGAGCTCTCATTTTGTCTCCGACGAGAGAGTTGGCTAGTCAGATATTTGAGGAAGCTGAGAAGTTTGCTTATCAGAGTGGGGCAAAAGTGGCTGTTGTTTATGGTGGAGCGCCGATGGGTCAGCAGCTTCGTGATCTGGAGAGAGGTGTTGACATCCTAGTCGCCACTCCAGGGCGCTTAGTGGACATGATTGAAAGGTCACGAGTTTCACTCAAGATGATCAAATACTTGGCACTAGATGAGGCTGATAGGATGTTGGACATGGGTTTTGAACCTCAAATAAGGAAGATTGTTCAAAGAATGGACATGCCTCCCCCAGGTGCAAGACAAACACTGCTTTTCAGTGCAACATTCCCGCATGAAATACAGAGGCTTGCTGCAGATTTCCTCAAGAACTACATTTTTCTGACTGTTGGGAGAGTTGGGTCAAGCACTGATCTTATAGCACAAAAGGTTGAGTTTGTCAGGGATATGGACAAAATACAGCATTTATGTGGTCTTCTTAAGAACCAGAGGGACAATGAAACTCATGGAAATCTTGCTTTAACCTTGGTGTTTGTTGAGACCAAGAAAGGAGCAAATCAATTGGAAAACTGGTTGTCCAGGAATGGATTCCCAGCAATAGCAATACATGGTGACAAAGTGCAAATGGAGAGGGAACGAGCTTTGAGATTGTTCAAGTGTGGCACAACTCCAATACTGGTGGCAACAGATGTTGCTTCACGAGGTTTAGATATCCCTCATGTTGCCCATGTCATCAATTTTGACTTCCCAAAAAGCATAGATGATTATGTTCACCGGATTGGTCGGACTGGACGTGCTGGTAAATCTGGTCTTGCAACTGCTTTCTTCAGTGAAAAGAACCAGCCTCTTGCAAAGGCACTAGTGGAGTTAATGAAGGAGTCAAAGCAGGAGGTTCCTAATTGGCTTAATGATTATGCTGAACAGTCATTGTCTTATGGTGGAGACCAGAGCCAAAGGTACAGTAGCAGTAACTTTGGTGGATATGACGTCCGTAGGAGTGAGAATTATTATGGTTCAGGCAGTTATGGTTATGGAGATTCTTCTGTGGGTGCGGCTGCTGCTACTTGTAATGATGTTTCTGGTTCATATGCTACTGGTTCATTTGGCCATGGAGGAAATTATGGGAATTCTTCTGTGGATGAGCCTGCTGCTTCCTACAATGGTTCTTATGGGGGTGAAACTGATGCTGCTGGTACTGCTGGTTCATCGAGCTTTGGAAATTATGAAAATATTGTTGCTGGTGGGTGGGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04622 ko05161 ko05203 map04622 map05161 map05203

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

661

Amino Acids

71.02

Weight (kDa)

6.85

Isoelectric Point (pI)

37.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	179 - 360	2.3e-50	DEAD/DEAH box helicase
Helicase_C	PF00271	396 - 511	1.8e-29	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42520 AT2G42520 AT2G42520 AT3G58510 AT3G58510 AT3G58510 AT3G58570
fragaria_vesca	FvH4_1g03520 FvH4_1g03520 FvH4_1g03520 FvH4_3g45200 FvH4_3g45200 FvH4_3g45200 FvH4_6g21570
malus_domestica	MD02G1034300.v1.1 MD03G1007400.v1.1 MD11G1010700.v1.1 MD12G1053000.v1.1 MD14G1051400.v1.1 MD15G1175000.v1.1
prunus_persica	Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.7G084600_v2.0.a1 Prupe.7G240900_v2.0.a1
pyrus_communis	pycom02g02800 pycom03g00760 pycom11g00670 pycom12g04630 pycom14g04260 pycom15g15650
rosa_chinensis	RchiOBHm_Chr2g0088731 RchiOBHm_Chr2g0088781 RchiOBHm_Chr2g0088941 RchiOBHm_Chr3g0478811 RchiOBHm_Chr5g0082811
rosa_laevigata	RLG00000015980 RLG00000016349 RLG00000023623 RLG00000036991
rosa_multiflora	Rmu_co8138978.1_g000001 Rmu_co8475903.1_g000001 Rmu_sc0000965.1_g000010 Rmu_sc0001381.1_g000020 Rmu_sc0001519.1_g000001 Rmu_sc0005751.1_g000004 Rmu_sc0011683.1_g000004
rosa_roxburghii	Rroxscaffold_1G00001630 Rroxscaffold_2G00148710 Rroxscaffold_6G00403050
rosa_rugosa	Rorug01G0486600 Rorug03G0168400 Rorug05G0479900 Rorug05G0479900 Rorug05G0479900 Rorug05G0480000
rosa_samantha	Rh2AG041800 Rh2AG076200 Rh2BG040500 Rh2BG077600 Rh2CG041300 Rh2CG041900 Rh2CG079800 Rh2DG040900 Rh2DG074900 Rh3AG220200 Rh3BG252000 Rh3DG245100 Rh5AG530300 Rh5DG565600
rosa_wichuraiana	Rw2G003260 Rw2G006990 Rw3G019790 Rw5G049570

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	261, 866
AatII	GACGTC	2 cut(s)	384, 1722
AccB1I	GGYRCC	1 cut(s)	454
AccBSI	CCGCTC	2 cut(s)	234, 438
AccI	GTMKAC	1 cut(s)	176
AccII	CGCG	3 cut(s)	79, 246, 554
AccIII	TCCGGA	1 cut(s)	608
AcoI	YGGCCR	6 cut(s)	157, 226, 280, 570, 574, 1837
AcsI	RAATTY	3 cut(s)	347, 400, 1858
AcuI	CTGAAG	1 cut(s)	1543
AcyI	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
AfaI	GTAC	4 cut(s)	185, 524, 1691, 1927
AfiI	CCNNNNNNNGG	2 cut(s)	879, 1025
AflII	CTTAAG	1 cut(s)	1211
AflIII	ACRYGT	1 cut(s)	336
AgsI	TTSAA	5 cut(s)	499, 902, 980, 1004, 1387
AhlI	ACTAGT	1 cut(s)	1591
AjiI	CACGTC	3 cut(s)	337, 448, 1526
AjnI	CCWGG	3 cut(s)	877, 1024, 1310
AluBI	AGCT	7 cut(s)	150, 358, 713, 764, 835, 1373, 1944
AluI	AGCT	7 cut(s)	150, 358, 713, 764, 835, 1373, 1944
Alw21I	GWGCWC	3 cut(s)	467, 598, 715
Alw26I	GTCTC	4 cut(s)	678, 729, 1265, 1668
Aor13HI	TCCGGA	1 cut(s)	608
AoxI	GGCC	8 cut(s)	24, 157, 226, 280, 311, 570, 574, 1837
ApeKI	GCWGC	7 cut(s)	355, 832, 1085, 1787, 1790, 1880, 1919
ApoI	RAATTY	3 cut(s)	347, 400, 1858
ArsI	GACNNNNNNTTYG	2 cut(s)	959, 991
Asp700I	GAANNNNTTC	1 cut(s)	1319
AspLEI	GCGC	5 cut(s)	81, 457, 549, 820, 885
AspS9I	GGNCC	3 cut(s)	24, 236, 606
AsuC2I	CCSGG	5 cut(s)	49, 240, 343, 578, 604
AsuHPI	GGTGA	4 cut(s)	46, 1355, 1496, 1919
AvaII	GGWCC	2 cut(s)	236, 606
AxyI	CCTNAGG	1 cut(s)	8
BalI	TGGCCA	1 cut(s)	1839
BanI	GGYRCC	1 cut(s)	454
BanII	GRGCYC	1 cut(s)	715
BauI	CACGAG	2 cut(s)	909, 1431
BbsI	GAAGAC	1 cut(s)	1199
Bbv12I	GWGCWC	3 cut(s)	467, 598, 715
BbvI	GCAGC	7 cut(s)	367, 844, 1072, 1774, 1777, 1867, 1906
BccI	CCATC	5 cut(s)	10, 128, 580, 683, 817
BceAI	ACGGC	3 cut(s)	144, 172, 633
BciT130I	CCWGG	3 cut(s)	879, 1026, 1312
BclI	TGATCA	1 cut(s)	927
BcnI	CCSGG	5 cut(s)	49, 240, 343, 578, 604
BcoDI	GTCTC	4 cut(s)	678, 729, 1265, 1668
BcuI	ACTAGT	1 cut(s)	1591
BfaI	CTAG	5 cut(s)	168, 744, 866, 944, 1592
BfmI	CTRYAG	1 cut(s)	1086
BfoI	RGCGCY	4 cut(s)	458, 550, 821, 886
BfrI	CTTAAG	1 cut(s)	1211
Bme1390I	CCNGG	8 cut(s)	49, 240, 343, 578, 604, 879, 1026, 1312
Bme18I	GGWCC	2 cut(s)	236, 606
BmgBI	CACGTC	3 cut(s)	337, 448, 1526
BmgT120I	GGNCC	3 cut(s)	24, 236, 606
BmiI	GGNNCC	3 cut(s)	456, 607, 1626
BmrFI	CCNGG	8 cut(s)	49, 240, 343, 578, 604, 879, 1026, 1312
BmsI	GCATC	3 cut(s)	112, 397, 1906
BpiI	GAAGAC	1 cut(s)	1199
BplI	GAGNNNNNCTC	2 cut(s)	903, 935
BpmI	CTGGAG	2 cut(s)	861, 866
BpuEI	CTTGAG	2 cut(s)	905, 1082
BpuMI	CCSGG	5 cut(s)	49, 240, 343, 578, 604
BsaAI	YACGTR	1 cut(s)	526
BsaHI	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
BsaI	GGTCTC	3 cut(s)	678, 1265, 1668
BsaJI	CCNNGG	7 cut(s)	160, 308, 567, 878, 1024, 1257, 1840
BsaWI	WCCGGW	3 cut(s)	424, 608, 1506
BsaXI	ACNNNNNCTCC	2 cut(s)	277, 307
Bsc4I	CCNNNNNNNGG	2 cut(s)	879, 1025
Bse118I	RCCGGY	4 cut(s)	451, 457, 538, 572
Bse1I	ACTGG	4 cut(s)	1307, 1413, 1525, 1831
Bse21I	CCTNAGG	1 cut(s)	8
Bse3DI	GCAATG	1 cut(s)	394
BseAI	TCCGGA	1 cut(s)	608
BseBI	CCWGG	3 cut(s)	879, 1026, 1312
BseDI	CCNNGG	7 cut(s)	160, 308, 567, 878, 1024, 1257, 1840
BseGI	GGATG	5 cut(s)	382, 694, 861, 966, 1876
BseLI	CCNNNNNNNGG	2 cut(s)	879, 1025
BseMI	GCAATG	1 cut(s)	394
BseMII	CTCAG	4 cut(s)	194, 611, 689, 756
BseNI	ACTGG	4 cut(s)	1307, 1413, 1525, 1831
BseRI	GAGGAG	1 cut(s)	273
BseX3I	CGGCCG	3 cut(s)	157, 226, 574
BseXI	GCAGC	7 cut(s)	367, 844, 1072, 1774, 1777, 1867, 1906
BseYI	CCCAGC	1 cut(s)	1324
BsgI	GTGCAG	1 cut(s)	320
Bsh1236I	CGCG	3 cut(s)	79, 246, 554
Bsh1285I	CGRYCG	3 cut(s)	160, 229, 577
BshFI	GGCC	8 cut(s)	26, 159, 228, 282, 313, 572, 576, 1839
BshNI	GGYRCC	1 cut(s)	454
BsiEI	CGRYCG	3 cut(s)	160, 229, 577
BsiHKAI	GWGCWC	3 cut(s)	467, 598, 715
BslFI	GGGAC	2 cut(s)	593, 1238
BslI	CCNNNNNNNGG	2 cut(s)	879, 1025
BsmAI	GTCTC	4 cut(s)	678, 729, 1265, 1668
BsmFI	GGGAC	2 cut(s)	593, 1238
BsnI	GGCC	8 cut(s)	26, 159, 228, 282, 313, 572, 576, 1839
Bso31I	GGTCTC	3 cut(s)	678, 1265, 1668
Bsp1286I	GDGCHC	3 cut(s)	467, 598, 715
Bsp13I	TCCGGA	1 cut(s)	608
Bsp143I	GATC	3 cut(s)	841, 927, 1144
Bsp19I	CCATGG	2 cut(s)	567, 1840
Bsp68I	TCGCGA	1 cut(s)	246
BspANI	GGCC	8 cut(s)	26, 159, 228, 282, 313, 572, 576, 1839
BspCNI	CTCAG	4 cut(s)	193, 610, 688, 757
BspEI	TCCGGA	1 cut(s)	608
BspFNI	CGCG	3 cut(s)	79, 246, 554
BspHI	TCATGA	1 cut(s)	331
BspLI	GGNNCC	3 cut(s)	456, 607, 1626
BspMAI	CTGCAG	1 cut(s)	1090
BspT107I	GGYRCC	1 cut(s)	454
BspTI	CTTAAG	1 cut(s)	1211
BspTNI	GGTCTC	3 cut(s)	678, 1265, 1668
BsrBI	CCGCTC	2 cut(s)	234, 438
BsrDI	GCAATG	1 cut(s)	394
BsrFI	RCCGGY	4 cut(s)	451, 457, 538, 572
BsrI	ACTGG	4 cut(s)	1307, 1413, 1525, 1831
BssAI	RCCGGY	4 cut(s)	451, 457, 538, 572
BssECI	CCNNGG	7 cut(s)	160, 308, 567, 878, 1024, 1257, 1840
BssMI	GATC	3 cut(s)	841, 927, 1144
BssNI	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
BssSI	CACGAG	2 cut(s)	909, 1431
BssT1I	CCWWGG	3 cut(s)	567, 1257, 1840
Bst2BI	CACGAG	2 cut(s)	909, 1431
Bst2UI	CCWGG	3 cut(s)	879, 1026, 1312
Bst4CI	ACNGT	5 cut(s)	188, 700, 1120, 1656, 1694
BstACI	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
BstAFI	CTTAAG	1 cut(s)	1211
BstBAI	YACGTR	1 cut(s)	526
BstC8I	GCNNGC	7 cut(s)	436, 453, 459, 463, 574, 1083, 1878
BstDEI	CTNAG	7 cut(s)	8, 180, 597, 657, 675, 765, 886
BstDSI	CCRYGG	3 cut(s)	160, 567, 1840
BstEII	GGTNACC	1 cut(s)	34
BstENI	CCTNNNNNAGG	1 cut(s)	1023
BstF5I	GGATG	5 cut(s)	382, 694, 861, 966, 1876
BstFNI	CGCG	3 cut(s)	79, 246, 554
BstH2I	RGCGCY	4 cut(s)	458, 550, 821, 886
BstHHI	GCGC	5 cut(s)	81, 457, 549, 820, 885
BstKTI	GATC	3 cut(s)	844, 930, 1147
BstMAI	GTCTC	4 cut(s)	678, 729, 1265, 1668
BstMBI	GATC	3 cut(s)	841, 927, 1144
BstMCI	CGRYCG	3 cut(s)	160, 229, 577
BstMWI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 231, 544, 797, 1790, 1928
BstNI	CCWGG	3 cut(s)	879, 1026, 1312
BstNSI	RCATGY	1 cut(s)	1018
BstPI	GGTNACC	1 cut(s)	34
BstSCI	CCNGG	8 cut(s)	47, 238, 341, 576, 602, 877, 1024, 1310
BstSFI	CTRYAG	1 cut(s)	1086
BstUI	CGCG	3 cut(s)	79, 246, 554
BstV1I	GCAGC	7 cut(s)	367, 844, 1072, 1774, 1777, 1867, 1906
BstV2I	GAAGAC	1 cut(s)	1199
BstZI	CGGCCG	3 cut(s)	157, 226, 574
Bsu36I	CCTNAGG	1 cut(s)	8
BsuRI	GGCC	8 cut(s)	26, 159, 228, 282, 313, 572, 576, 1839
BtgI	CCRYGG	3 cut(s)	160, 567, 1840
BtgZI	GCGATG	1 cut(s)	147
BtrI	CACGTC	3 cut(s)	337, 448, 1526
BtsCI	GGATG	5 cut(s)	382, 694, 861, 966, 1876
BtsI	GCAGTG	1 cut(s)	1040
BtsIMutI	CAGTG	5 cut(s)	649, 1040, 1057, 1140, 1567
BtuMI	TCGCGA	1 cut(s)	246
Cac8I	GCNNGC	7 cut(s)	436, 453, 459, 463, 574, 1083, 1878
CciI	TCATGA	1 cut(s)	331
CfoI	GCGC	5 cut(s)	81, 457, 549, 820, 885
Cfr10I	RCCGGY	4 cut(s)	451, 457, 538, 572
Cfr13I	GGNCC	3 cut(s)	24, 236, 606
CpoI	CGGWCCG	1 cut(s)	236
CseI	GACGC	2 cut(s)	313, 544
Csp6I	GTAC	4 cut(s)	184, 523, 1690, 1926
CspCI	CAANNNNNGTGG	2 cut(s)	1392, 1427
CspI	CGGWCCG	1 cut(s)	236
CviQI	GTAC	4 cut(s)	184, 523, 1690, 1926
DdeI	CTNAG	7 cut(s)	8, 180, 597, 657, 675, 765, 886
DinI	GGCGCC	1 cut(s)	456
DpnI	GATC	3 cut(s)	843, 929, 1146
DpnII	GATC	3 cut(s)	841, 927, 1144
DrdI	GACNNNNNNGTC	2 cut(s)	261, 866
DseDI	GACNNNNNNGTC	2 cut(s)	261, 866
EaeI	YGGCCR	6 cut(s)	157, 226, 280, 570, 574, 1837
EagI	CGGCCG	3 cut(s)	157, 226, 574
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	713
EclXI	CGGCCG	3 cut(s)	157, 226, 574
Eco130I	CCWWGG	3 cut(s)	567, 1257, 1840
Eco24I	GRGCYC	1 cut(s)	715
Eco31I	GGTCTC	3 cut(s)	678, 1265, 1668
Eco32I	GATATC	1 cut(s)	1444
Eco47I	GGWCC	2 cut(s)	236, 606
Eco52I	CGGCCG	3 cut(s)	157, 226, 574
Eco53kI	GAGCTC	1 cut(s)	713
Eco57I	CTGAAG	1 cut(s)	1543
Eco81I	CCTNAGG	1 cut(s)	8
Eco91I	GGTNACC	1 cut(s)	34
EcoICRI	GAGCTC	1 cut(s)	713
EcoNI	CCTNNNNNAGG	1 cut(s)	1023
EcoO65I	GGTNACC	1 cut(s)	34
EcoRI	GAATTC	1 cut(s)	1858
EcoRII	CCWGG	3 cut(s)	877, 1024, 1310
EcoRV	GATATC	1 cut(s)	1444
EcoT14I	CCWWGG	3 cut(s)	567, 1257, 1840
EcoT38I	GRGCYC	1 cut(s)	715
EgeI	GGCGCC	1 cut(s)	456
EheI	GGCGCC	1 cut(s)	456
ErhI	CCWWGG	3 cut(s)	567, 1257, 1840
FaqI	GGGAC	2 cut(s)	593, 1238
FauI	CCCGC	2 cut(s)	81, 1071
FauNDI	CATATG	1 cut(s)	1819
FbaI	TGATCA	1 cut(s)	927
FblI	GTMKAC	1 cut(s)	176
FokI	GGATG	5 cut(s)	389, 701, 848, 973, 1883
FriOI	GRGCYC	1 cut(s)	715
FseI	GGCCGGCC	1 cut(s)	576
FspBI	CTAG	5 cut(s)	168, 744, 866, 944, 1592
GlaI	GCGC	5 cut(s)	80, 456, 548, 819, 884
GsaI	CCCAGC	1 cut(s)	1328
GsuI	CTGGAG	2 cut(s)	861, 866
HaeII	RGCGCY	4 cut(s)	458, 550, 821, 886
HaeIII	GGCC	8 cut(s)	26, 159, 228, 282, 313, 572, 576, 1839
HgaI	GACGC	2 cut(s)	313, 544
HhaI	GCGC	5 cut(s)	81, 457, 549, 820, 885
Hin1I	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
Hin6I	GCGC	5 cut(s)	79, 455, 547, 818, 883
HinP1I	GCGC	5 cut(s)	79, 455, 547, 818, 883
HincII	GTYRAC	3 cut(s)	177, 654, 859
HindII	GTYRAC	3 cut(s)	177, 654, 859
HinfI	GANTC	8 cut(s)	41, 173, 178, 286, 556, 1320, 1610, 1769
HphI	GGTGA	4 cut(s)	46, 1355, 1496, 1919
Hpy166II	GTNNAC	5 cut(s)	177, 654, 859, 892, 1504
Hpy188I	TCNGA	8 cut(s)	561, 600, 678, 729, 750, 783, 1116, 1518
Hpy8I	GTNNAC	5 cut(s)	177, 654, 859, 892, 1504
Hpy99I	CGWCG	4 cut(s)	251, 386, 538, 733
HpyAV	CCTTC	1 cut(s)	1600
HpyCH4III	ACNGT	5 cut(s)	188, 700, 1120, 1656, 1694
HpyCH4IV	ACGT	6 cut(s)	336, 381, 447, 525, 1525, 1719
HpyCH4V	TGCA	7 cut(s)	301, 1031, 1055, 1088, 1354, 1547, 1583
HpyF10VI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 231, 544, 797, 1790, 1928
HpyF3I	CTNAG	7 cut(s)	8, 180, 597, 657, 675, 765, 886
HpySE526I	ACGT	6 cut(s)	336, 381, 447, 525, 1525, 1719
Hsp92I	GRCGYC	5 cut(s)	305, 381, 455, 536, 1719
HspAI	GCGC	5 cut(s)	79, 455, 547, 818, 883
KasI	GGCGCC	1 cut(s)	454
Kpn2I	TCCGGA	1 cut(s)	608
KroI	GCCGGC	3 cut(s)	451, 457, 572
KroNI	GCCGGC	3 cut(s)	453, 459, 574
Ksp22I	TGATCA	1 cut(s)	927
Kzo9I	GATC	3 cut(s)	841, 927, 1144
LmnI	GCTCC	4 cut(s)	155, 231, 815, 1283
Lsp1109I	GCAGC	7 cut(s)	367, 844, 1072, 1774, 1777, 1867, 1906
LweI	GCATC	3 cut(s)	112, 397, 1906
MaeI	CTAG	5 cut(s)	168, 744, 866, 944, 1592
MaeII	ACGT	6 cut(s)	336, 381, 447, 525, 1525, 1719
MaeIII	GTNAC	6 cut(s)	34, 143, 212, 906, 1343, 1700
MalI	GATC	3 cut(s)	843, 929, 1146
MbiI	CCGCTC	2 cut(s)	234, 438
MboI	GATC	3 cut(s)	841, 927, 1144
MboII	GAAGA	6 cut(s)	98, 1006, 1199, 1549, 1764, 1854
MfeI	CAATTG	1 cut(s)	1292
MhlI	GDGCHC	3 cut(s)	467, 598, 715
MlsI	TGGCCA	1 cut(s)	1839
MluCI	AATT	9 cut(s)	347, 400, 1292, 1468, 1630, 1735, 1849, 1858, 1951
MluNI	TGGCCA	1 cut(s)	1839
Mly113I	GGCGCC	1 cut(s)	455
MlyI	GAGTC	5 cut(s)	50, 172, 182, 295, 1619
MmeI	TCCRAC	4 cut(s)	674, 752, 945, 1496
Mox20I	TGGCCA	1 cut(s)	1839
MreI	CGCCGGCG	1 cut(s)	457
MroI	TCCGGA	1 cut(s)	608
MroNI	GCCGGC	3 cut(s)	451, 457, 572
MroXI	GAANNNNTTC	1 cut(s)	1319
MscI	TGGCCA	1 cut(s)	1839
MseI	TTAA	4 cut(s)	1212, 1253, 1601, 1638
MslI	CAYNNNNRTG	1 cut(s)	1200
Msp20I	TGGCCA	1 cut(s)	1839
MspA1I	CMGCKG	1 cut(s)	358
MspCI	CTTAAG	1 cut(s)	1211
MspR9I	CCNGG	8 cut(s)	49, 240, 343, 578, 604, 879, 1026, 1312
MunI	CAATTG	1 cut(s)	1292
MvaI	CCWGG	3 cut(s)	879, 1026, 1312
MvnI	CGCG	3 cut(s)	79, 246, 554
MwoI	GCNNNNNNNGC	9 cut(s)	117, 126, 129, 156, 231, 544, 797, 1790, 1928
NaeI	GCCGGC	3 cut(s)	453, 459, 574
NarI	GGCGCC	1 cut(s)	455
NciI	CCSGG	5 cut(s)	49, 240, 343, 578, 604
NcoI	CCATGG	2 cut(s)	567, 1840
NdeI	CATATG	1 cut(s)	1819
NdeII	GATC	3 cut(s)	841, 927, 1144
NgoMIV	GCCGGC	3 cut(s)	451, 457, 572
NlaIV	GGNNCC	3 cut(s)	456, 607, 1626
NmeAIII	GCCGAG	1 cut(s)	333
NmuCI	GTSAC	3 cut(s)	34, 906, 1343
NruI	TCGCGA	1 cut(s)	246
NspI	RCATGY	1 cut(s)	1018
PagI	TCATGA	1 cut(s)	331
PcsI	WCGNNNNNNNCGW	4 cut(s)	262, 311, 411, 1522
PdiI	GCCGGC	3 cut(s)	453, 459, 574
PdmI	GAANNNNTTC	1 cut(s)	1319
PfeI	GAWTC	3 cut(s)	556, 1320, 1769
PflFI	GACNNNGTC	2 cut(s)	40, 604
PfoI	TCCNGGA	2 cut(s)	341, 1310
PleI	GAGTC	5 cut(s)	49, 172, 181, 294, 1618
PluTI	GGCGCC	1 cut(s)	458
PpsI	GAGTC	5 cut(s)	49, 172, 181, 294, 1618
Ppu21I	YACGTR	1 cut(s)	526
Psp124BI	GAGCTC	1 cut(s)	715
Psp6I	CCWGG	3 cut(s)	877, 1024, 1310
PspEI	GGTNACC	1 cut(s)	34
PspFI	CCCAGC	1 cut(s)	1324
PspGI	CCWGG	3 cut(s)	877, 1024, 1310
PspN4I	GGNNCC	3 cut(s)	456, 607, 1626
PspPI	GGNCC	3 cut(s)	24, 236, 606
PsrI	GAACNNNNNNTAC	2 cut(s)	1918, 1950
PstI	CTGCAG	1 cut(s)	1090
PsyI	GACNNNGTC	2 cut(s)	40, 604
PvuII	CAGCTG	1 cut(s)	358
RigI	GGCCGGCC	1 cut(s)	576
RruI	TCGCGA	1 cut(s)	246
RsaI	GTAC	4 cut(s)	185, 524, 1691, 1927
RsaNI	GTAC	4 cut(s)	184, 523, 1690, 1926
RseI	CAYNNNNRTG	1 cut(s)	1200
Rsr2I	CGGWCCG	1 cut(s)	236
RsrII	CGGWCCG	1 cut(s)	236
SacI	GAGCTC	1 cut(s)	715
SalI	GTCGAC	1 cut(s)	175
SaqAI	TTAA	4 cut(s)	1212, 1253, 1601, 1638
Sau3AI	GATC	3 cut(s)	841, 927, 1144
Sau96I	GGNCC	3 cut(s)	24, 236, 606
SchI	GAGTC	5 cut(s)	50, 172, 182, 295, 1619
ScrFI	CCNGG	8 cut(s)	49, 240, 343, 578, 604, 879, 1026, 1312
SduI	GDGCHC	3 cut(s)	467, 598, 715
SfaNI	GCATC	3 cut(s)	112, 397, 1906
SfcI	CTRYAG	1 cut(s)	1086
SfoI	GGCGCC	1 cut(s)	456
SgrAI	CRCCGGYG	1 cut(s)	457
SinI	GGWCC	2 cut(s)	236, 606
SmiMI	CAYNNNNRTG	1 cut(s)	1200
SmlI	CTYRAG	3 cut(s)	920, 1097, 1211
SmoI	CTYRAG	3 cut(s)	920, 1097, 1211
SpeI	ACTAGT	1 cut(s)	1591
Sse9I	AATT	9 cut(s)	347, 400, 1292, 1468, 1630, 1735, 1849, 1858, 1951
SspDI	GGCGCC	1 cut(s)	454
SspI	AATATT	1 cut(s)	1963
SspMI	CTAG	5 cut(s)	168, 744, 866, 944, 1592
SstI	GAGCTC	1 cut(s)	715
StyD4I	CCNGG	8 cut(s)	47, 238, 341, 576, 602, 877, 1024, 1310
StyI	CCWWGG	3 cut(s)	567, 1257, 1840
TaaI	ACNGT	5 cut(s)	188, 700, 1120, 1656, 1694
TaiI	ACGT	6 cut(s)	339, 384, 450, 528, 1528, 1722
TaqI	TCGA	4 cut(s)	176, 289, 405, 1940
TaqII	GACCGA	1 cut(s)	53
TasI	AATT	9 cut(s)	347, 400, 1292, 1468, 1630, 1735, 1849, 1858, 1951
TauI	GCSGC	9 cut(s)	79, 123, 126, 129, 132, 228, 441, 623, 1787
TfiI	GAWTC	3 cut(s)	556, 1320, 1769
Tru1I	TTAA	4 cut(s)	1212, 1253, 1601, 1638
Tru9I	TTAA	4 cut(s)	1212, 1253, 1601, 1638
TscAI	CASTG	5 cut(s)	649, 1047, 1057, 1147, 1567
TseFI	GTSAC	3 cut(s)	34, 906, 1343
TseI	GCWGC	7 cut(s)	355, 832, 1085, 1787, 1790, 1880, 1919
Tsp45I	GTSAC	3 cut(s)	34, 906, 1343
TspDTI	ATGAA	8 cut(s)	320, 1083, 1245, 1619, 1806, 1821, 1926, 1971
TspGWI	ACGGA	2 cut(s)	264, 1712
TspRI	CASTG	5 cut(s)	649, 1047, 1057, 1147, 1567
Tth111I	GACNNNGTC	2 cut(s)	40, 604
Vha464I	CTTAAG	1 cut(s)	1211
VpaK11BI	GGWCC	2 cut(s)	236, 606
XagI	CCTNNNNNAGG	1 cut(s)	1023
XapI	RAATTY	3 cut(s)	347, 400, 1858
XceI	RCATGY	1 cut(s)	1018
XmiI	GTMKAC	1 cut(s)	176
XmnI	GAANNNNTTC	1 cut(s)	1319
XspI	CTAG	5 cut(s)	168, 744, 866, 944, 1592
ZraI	GACGTC	2 cut(s)	382, 1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.