Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000684

Physical Location & Seq

Genomic Coordinates Forward (+)

86066382 .. 86072263

Gene Structure 5882 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G049570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGCGAGCTTCATGGGCAGATTTGGCTGCAAATTCTGCAGCTGAGAATGTAGGTTCTGGGTCTTCTGCTAACAGCGCTGCCGGTGGTGCTCCCACTGCTGGTGCTCCGTCGCGGTCCACCTACGTCCCCCCGCATCTGCGTAACCGTCCGCCTTCATCGGACCCACCTGCTCCAGCGTATAGTGCGCCTGTGGGGAATGATCGAGGTGGGTATGGTGGATCTCGTTGGGGTGGCGGCAGGAATGACAACAGTCGGTCTGGGTATGGAGGGAGCGGCGGCGGCGGCGGCCGCGGTGGGGGATGGGGTGGCGGCAGAGGTGGCGGTTGGGACCGTGGGAGGGACAGGGAAGTGAACCCCTTTGAAGATGATGACGAAACAGAGCAGCCGTTTAGTGAGCAGGAGAACAGTGGCATTAACTTTGATGCTTATGAAGACATTCCTGTGGAGACAAGTGGTGACAATGTTCCACCCCCTGTGAATACATTTGCAGAGATTGACTTGGGTGACGCACTTAATAAAAATATTCAGAGGTGCAAATATGTGAAACCAACGCCTGTGCAGCGGCATGCCATTCCAATTTCCCTTGGAGGTCGGGACTTGATGGCATGTGCTCAAACTGGTTCTGGCAAGACAGCTGCTTTCTGTTTCCCTATCATTAGTGGAATCATGAAGGGCCAACCTCCACAGAGACCGCCCCGTTCACATACAGCCTGCCCACTTGCTCTCATTTTATCTCCTACTAGGGAGCTTTCTTCTCAAATACACGAGGAAGCTAAGAAATTTTCATATCAAACTGGTGTCAGAGCTGTTGTTGCTTATGGAGGAGCTCCAATTCATCAGCAGCTGCGGGAACTGGAAAGAGGTGTTGATATTCTTGTGGCAACTCCTGGAAGATTGGTGGATTTGCTGGAGAGAGCTAGAGTGTCATTGCAGAATATTAGGTATTTAGCCCTTGATGAGGCTGATCGAATGCTGGACATGGGTTTTGAACCGCAAATAAGGAAAATTGTGGAACAAATGGACATGCCTCCACCAGGTGTGCGACAGACTATGTTGTTCAGTGCCACTTTTCCAAAAGAAATACAGAGACTGGCCTCTGATTTTCTTTCCAAGTACATCTTTCTGGCTGTTGGAAGGGTGGGTTCTAGTACTGATTTGATTGTCCAACGAGTGGAATTTGTTCATGAATCTGATAAAAGAAGTCACCTTATGGACCTTCTGCATGCACAAAGGGCCAATGGTACACAGGGGAAGCAAGCTCTGACGTTAGTTTTTGTGGAGACAAAGAAGGGAGCTGATTCCCTGGAACACTGGTTGTGTATGAATGGTTTTCCTGCAACTACTATTCATGGCGACAGAACACAGCAGGAAAGAGAACAAGCATTGAGGTCATTTAAGAGCGGCAATACTCCAATTTTGGTGGCTACTGATGTTGCTGCACGTGGGCTTGATATTCCTCACGTTGCACATGTTGTCAACTTTGATCTTCCAAATGACATTGATGATTATGTTCACCGCATTGGTCGTACTGGACGAGCTGGGAAATCTGGTTTGGCTACTGCCTTCTTTAATGAGAACAATTCATCACTGGCCAAGTCTCTAGCAGATTTGATGCAAGAATCAAATCAAGAAGTACCTGCTTGGTTGTCACGGTATGCAGCTCGATCCTCTTACGTGGTGGGAGGAACCGCCGTTCTGGGGGAGGTCGGTTTGGTGGCCGTGACTTTCGAAGGGATTCCTATAGTGGTGGTGGCGGCGGCGGCGGCGGATATGGCGGTTCTGGTGGGTATGGTGGAGGAGGATATGGGGGTTCTGGTGGGTTTAGTGGAGGAGGAGGTTATGGTGCTGGGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko04622 ko05161 ko05203 map04622 map05161 map05203

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

617

Amino Acids

65.99

Weight (kDa)

5.64

Isoelectric Point (pI)

44.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	184 - 363	5.6e-50	DEAD/DEAH box helicase
Helicase_C	PF00271	399 - 514	9.1e-32	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42520 AT2G42520 AT2G42520 AT3G58510 AT3G58510 AT3G58510 AT3G58570
fragaria_vesca	FvH4_1g03520 FvH4_1g03520 FvH4_1g03520 FvH4_3g45200 FvH4_3g45200 FvH4_3g45200 FvH4_6g21570
malus_domestica	MD02G1034300.v1.1 MD03G1007400.v1.1 MD11G1010700.v1.1 MD12G1053000.v1.1 MD14G1051400.v1.1 MD15G1175000.v1.1
prunus_persica	Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.6G007000_v2.0.a1 Prupe.7G084600_v2.0.a1 Prupe.7G240900_v2.0.a1
pyrus_communis	pycom02g02800 pycom03g00760 pycom11g00670 pycom12g04630 pycom14g04260 pycom15g15650
rosa_chinensis	RchiOBHm_Chr2g0088731 RchiOBHm_Chr2g0088781 RchiOBHm_Chr2g0088941 RchiOBHm_Chr3g0478811 RchiOBHm_Chr5g0082811
rosa_laevigata	RLG00000015980 RLG00000016349 RLG00000023623 RLG00000036991
rosa_multiflora	Rmu_co8138978.1_g000001 Rmu_co8475903.1_g000001 Rmu_sc0000965.1_g000010 Rmu_sc0001381.1_g000020 Rmu_sc0001519.1_g000001 Rmu_sc0005751.1_g000004 Rmu_sc0011683.1_g000004
rosa_roxburghii	Rroxscaffold_1G00001630 Rroxscaffold_2G00148710 Rroxscaffold_6G00403050
rosa_rugosa	Rorug01G0486600 Rorug03G0168400 Rorug05G0479900 Rorug05G0479900 Rorug05G0479900 Rorug05G0480000
rosa_samantha	Rh2AG041800 Rh2AG076200 Rh2BG040500 Rh2BG077600 Rh2CG041300 Rh2CG041900 Rh2CG079800 Rh2DG040900 Rh2DG074900 Rh3AG220200 Rh3BG252000 Rh3DG245100 Rh5AG530300 Rh5DG565600
rosa_wichuraiana	Rw2G003260 Rw2G006990 Rw3G019790 Rw5G049570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	175
Acc36I	ACCTGC	2 cut(s)	175, 1645
AccB7I	CCANNNNNTGG	1 cut(s)	1171
AccBSI	CCGCTC	2 cut(s)	273, 1401
AccII	CGCG	2 cut(s)	112, 291
AclWI	GGATC	2 cut(s)	226, 1659
AcoI	YGGCCR	3 cut(s)	286, 1590, 1716
AcsI	RAATTY	3 cut(s)	31, 779, 1175
AcvI	CACGTG	1 cut(s)	1442
AdeI	CACNNNGTG	1 cut(s)	1037
AfaI	GTAC	5 cut(s)	1115, 1150, 1243, 1528, 1635
AfeI	AGCGCT	1 cut(s)	76
AfiI	CCNNNNNNNGG	7 cut(s)	98, 295, 337, 958, 1034, 1171, 1698
AflIII	ACRYGT	1 cut(s)	1468
AgsI	TTSAA	2 cut(s)	362, 989
AjnI	CCWGG	3 cut(s)	886, 1033, 1302
AjuI	GAANNNNNNNTTGG	2 cut(s)	1535, 1567
AloI	GAACNNNNNNTCC	2 cut(s)	1677, 1709
Alw21I	GWGCWC	4 cut(s)	91, 106, 613, 829
Alw26I	GTCTC	5 cut(s)	440, 682, 1081, 1274, 1602
AlwI	GGATC	2 cut(s)	226, 1659
AlwNI	CAGNNNCTG	2 cut(s)	844, 1090
Aor51HI	AGCGCT	1 cut(s)	76
AoxI	GGCC	6 cut(s)	286, 673, 1092, 1233, 1590, 1716
ApoI	RAATTY	3 cut(s)	31, 779, 1175
ArsI	GACNNNNNNTTYG	2 cut(s)	968, 1000
Asp700I	GAANNNNTTC	3 cut(s)	435, 1179, 1734
AspLEI	GCGC	2 cut(s)	77, 187
AspS9I	GGNCC	6 cut(s)	114, 160, 328, 673, 1213, 1233
AsuHPI	GGTGA	4 cut(s)	467, 515, 1196, 1505
AsuII	TTCGAA	1 cut(s)	1728
AvaII	GGWCC	4 cut(s)	114, 160, 328, 1213
BalI	TGGCCA	1 cut(s)	1592
BanII	GRGCYC	1 cut(s)	829
BauI	CACGAG	1 cut(s)	764
BbrPI	CACGTG	1 cut(s)	1442
BbsI	GAAGAC	2 cut(s)	54, 438
Bbv12I	GWGCWC	4 cut(s)	91, 106, 613, 829
BccI	CCATC	2 cut(s)	294, 595
BceAI	ACGGC	3 cut(s)	370, 1676, 1703
BciT130I	CCWGG	3 cut(s)	888, 1035, 1304
BcoDI	GTCTC	5 cut(s)	440, 682, 1081, 1274, 1602
BfaI	CTAG	4 cut(s)	741, 918, 1146, 1601
BfmI	CTRYAG	2 cut(s)	36, 1740
BfoI	RGCGCY	1 cut(s)	78
BfuAI	ACCTGC	2 cut(s)	175, 1645
BmcAI	AGTACT	1 cut(s)	1150
Bme1390I	CCNGG	3 cut(s)	888, 1035, 1304
Bme18I	GGWCC	4 cut(s)	114, 160, 328, 1213
BmgT120I	GGNCC	6 cut(s)	114, 160, 328, 673, 1213, 1233
BmiI	GGNNCC	3 cut(s)	162, 329, 1687
BmrFI	CCNGG	3 cut(s)	888, 1035, 1304
BmsI	GCATC	3 cut(s)	142, 412, 1602
BoxI	GACNNNNGTC	1 cut(s)	249
BpiI	GAAGAC	2 cut(s)	54, 438
BpmI	CTGGAG	2 cut(s)	156, 929
Bpu14I	TTCGAA	1 cut(s)	1728
BsaAI	YACGTR	2 cut(s)	1442, 1675
BsaI	GGTCTC	1 cut(s)	682
BsaJI	CCNNGG	4 cut(s)	289, 331, 583, 1302
Bsc4I	CCNNNNNNNGG	7 cut(s)	98, 295, 337, 958, 1034, 1171, 1698
Bse118I	RCCGGY	1 cut(s)	80
Bse1I	ACTGG	7 cut(s)	622, 799, 858, 1095, 1316, 1534, 1593
Bse3DI	GCAATG	1 cut(s)	926
BseBI	CCWGG	3 cut(s)	888, 1035, 1304
BseDI	CCNNGG	4 cut(s)	289, 331, 583, 1302
BseGI	GGATG	1 cut(s)	305
BseLI	CCNNNNNNNGG	7 cut(s)	98, 295, 337, 958, 1034, 1171, 1698
BseMI	GCAATG	1 cut(s)	926
BseMII	CTCAG	1 cut(s)	33
BseNI	ACTGG	7 cut(s)	622, 799, 858, 1095, 1316, 1534, 1593
BseRI	GAGGAG	4 cut(s)	837, 1811, 1844, 1847
BseX3I	CGGCCG	1 cut(s)	286
BseYI	CCCAGC	2 cut(s)	1538, 1846
BsgI	GTGCAG	2 cut(s)	578, 1422
Bsh1236I	CGCG	2 cut(s)	112, 291
Bsh1285I	CGRYCG	1 cut(s)	289
BshFI	GGCC	6 cut(s)	288, 675, 1094, 1235, 1592, 1718
BsiEI	CGRYCG	1 cut(s)	289
BsiHKAI	GWGCWC	4 cut(s)	91, 106, 613, 829
BsiSI	CCGG	1 cut(s)	81
BslFI	GGGAC	4 cut(s)	110, 341, 353, 608
BslI	CCNNNNNNNGG	7 cut(s)	98, 295, 337, 958, 1034, 1171, 1698
BsmAI	GTCTC	5 cut(s)	440, 682, 1081, 1274, 1602
BsmFI	GGGAC	4 cut(s)	110, 341, 353, 608
BsmI	GAATGC	1 cut(s)	975
BsnI	GGCC	6 cut(s)	288, 675, 1094, 1235, 1592, 1718
Bso31I	GGTCTC	1 cut(s)	682
Bsp119I	TTCGAA	1 cut(s)	1728
Bsp1286I	GDGCHC	4 cut(s)	91, 106, 613, 829
Bsp143I	GATC	5 cut(s)	199, 218, 964, 1483, 1664
BspANI	GGCC	6 cut(s)	288, 675, 1094, 1235, 1592, 1718
BspCNI	CTCAG	1 cut(s)	34
BspFNI	CGCG	2 cut(s)	112, 291
BspHI	TCATGA	2 cut(s)	666, 1183
BspLI	GGNNCC	3 cut(s)	162, 329, 1687
BspMAI	CTGCAG	1 cut(s)	40
BspMI	ACCTGC	2 cut(s)	175, 1645
BspPI	GGATC	2 cut(s)	226, 1659
BspT104I	TTCGAA	1 cut(s)	1728
BspTNI	GGTCTC	1 cut(s)	682
BsrBI	CCGCTC	2 cut(s)	273, 1401
BsrDI	GCAATG	1 cut(s)	926
BsrFI	RCCGGY	1 cut(s)	80
BsrI	ACTGG	7 cut(s)	622, 799, 858, 1095, 1316, 1534, 1593
BssAI	RCCGGY	1 cut(s)	80
BssECI	CCNNGG	4 cut(s)	289, 331, 583, 1302
BssMI	GATC	5 cut(s)	199, 218, 964, 1483, 1664
BssSI	CACGAG	1 cut(s)	764
BssT1I	CCWWGG	1 cut(s)	583
Bst2BI	CACGAG	1 cut(s)	764
Bst2UI	CCWGG	3 cut(s)	888, 1035, 1304
Bst4CI	ACNGT	5 cut(s)	146, 251, 332, 407, 1653
BstAPI	GCANNNNNTGC	1 cut(s)	35
BstBAI	YACGTR	2 cut(s)	1442, 1675
BstBI	TTCGAA	1 cut(s)	1728
BstC8I	GCNNGC	5 cut(s)	6, 567, 712, 1224, 1257
BstDEI	CTNAG	2 cut(s)	42, 774
BstDSI	CCRYGG	2 cut(s)	289, 331
BstENI	CCTNNNNNAGG	2 cut(s)	956, 1032
BstF5I	GGATG	1 cut(s)	305
BstFNI	CGCG	2 cut(s)	112, 291
BstH2I	RGCGCY	1 cut(s)	78
BstHHI	GCGC	2 cut(s)	77, 187
BstKTI	GATC	5 cut(s)	202, 221, 967, 1486, 1667
BstMAI	GTCTC	5 cut(s)	440, 682, 1081, 1274, 1602
BstMBI	GATC	5 cut(s)	199, 218, 964, 1483, 1664
BstMCI	CGRYCG	1 cut(s)	289
BstNI	CCWGG	3 cut(s)	888, 1035, 1304
BstNSI	RCATGY	5 cut(s)	569, 609, 1027, 1226, 1472
BstPAI	GACNNNNGTC	1 cut(s)	249
BstSCI	CCNGG	3 cut(s)	886, 1033, 1302
BstSFI	CTRYAG	2 cut(s)	36, 1740
BstUI	CGCG	2 cut(s)	112, 291
BstV2I	GAAGAC	2 cut(s)	54, 438
BstX2I	RGATCY	1 cut(s)	218
BstYI	RGATCY	1 cut(s)	218
BstZI	CGGCCG	1 cut(s)	286
BsuRI	GGCC	6 cut(s)	288, 675, 1094, 1235, 1592, 1718
BtgI	CCRYGG	2 cut(s)	289, 331
BtsCI	GGATG	1 cut(s)	305
BtsI	GCAGTG	1 cut(s)	93
BtsIMutI	CAGTG	5 cut(s)	93, 412, 1066, 1309, 1586
BveI	ACCTGC	2 cut(s)	175, 1645
Cac8I	GCNNGC	5 cut(s)	6, 567, 712, 1224, 1257
CaiI	CAGNNNCTG	2 cut(s)	844, 1090
CciI	TCATGA	2 cut(s)	666, 1183
CciNI	GCGGCCGC	1 cut(s)	286
CfoI	GCGC	2 cut(s)	77, 187
Cfr10I	RCCGGY	1 cut(s)	80
Cfr13I	GGNCC	6 cut(s)	114, 160, 328, 673, 1213, 1233
Cfr42I	CCGCGG	1 cut(s)	292
CseI	GACGC	1 cut(s)	515
CsiI	ACCWGGT	1 cut(s)	1033
Csp6I	GTAC	5 cut(s)	1114, 1149, 1242, 1527, 1634
CspCI	CAANNNNNGTGG	2 cut(s)	1401, 1436
CviQI	GTAC	5 cut(s)	1114, 1149, 1242, 1527, 1634
DdeI	CTNAG	2 cut(s)	42, 774
DpnI	GATC	5 cut(s)	201, 220, 966, 1485, 1666
DpnII	GATC	5 cut(s)	199, 218, 964, 1483, 1664
DraIII	CACNNNGTG	1 cut(s)	1037
EaeI	YGGCCR	3 cut(s)	286, 1590, 1716
EagI	CGGCCG	1 cut(s)	286
EciI	GGCGGA	2 cut(s)	138, 1781
Ecl136II	GAGCTC	1 cut(s)	827
EclXI	CGGCCG	1 cut(s)	286
Eco130I	CCWWGG	1 cut(s)	583
Eco24I	GRGCYC	1 cut(s)	829
Eco31I	GGTCTC	1 cut(s)	682
Eco47I	GGWCC	4 cut(s)	114, 160, 328, 1213
Eco47III	AGCGCT	1 cut(s)	76
Eco52I	CGGCCG	1 cut(s)	286
Eco53kI	GAGCTC	1 cut(s)	827
Eco72I	CACGTG	1 cut(s)	1442
EcoICRI	GAGCTC	1 cut(s)	827
EcoNI	CCTNNNNNAGG	2 cut(s)	956, 1032
EcoRII	CCWGG	3 cut(s)	886, 1033, 1302
EcoT14I	CCWWGG	1 cut(s)	583
EcoT38I	GRGCYC	1 cut(s)	829
ErhI	CCWWGG	1 cut(s)	583
FalI	AAGNNNNNCTT	2 cut(s)	1103, 1135
FaqI	GGGAC	4 cut(s)	110, 341, 353, 608
FauI	CCCGC	2 cut(s)	138, 840
FokI	GGATG	1 cut(s)	312
FriOI	GRGCYC	1 cut(s)	829
FspBI	CTAG	4 cut(s)	741, 918, 1146, 1601
GlaI	GCGC	2 cut(s)	76, 186
GsaI	CCCAGC	2 cut(s)	1542, 1850
GsuI	CTGGAG	2 cut(s)	156, 929
HaeII	RGCGCY	1 cut(s)	78
HaeIII	GGCC	6 cut(s)	288, 675, 1094, 1235, 1592, 1718
HapII	CCGG	1 cut(s)	81
HgaI	GACGC	1 cut(s)	515
HhaI	GCGC	2 cut(s)	77, 187
Hin6I	GCGC	2 cut(s)	75, 185
HinP1I	GCGC	2 cut(s)	75, 185
HincII	GTYRAC	1 cut(s)	1477
HindII	GTYRAC	1 cut(s)	1477
HinfI	GANTC	5 cut(s)	663, 1187, 1298, 1619, 1735
HpaII	CCGG	1 cut(s)	81
HphI	GGTGA	4 cut(s)	467, 515, 1196, 1505
Hpy166II	GTNNAC	6 cut(s)	117, 352, 701, 1244, 1477, 1513
Hpy188I	TCNGA	6 cut(s)	160, 528, 803, 1099, 1192, 1263
Hpy188III	TCNNGA	4 cut(s)	593, 667, 1184, 1628
Hpy8I	GTNNAC	6 cut(s)	117, 352, 701, 1244, 1477, 1513
Hpy99I	CGWCG	1 cut(s)	112
HpyAV	CCTTC	7 cut(s)	162, 664, 1128, 1226, 1282, 1573, 1724
HpyCH4III	ACNGT	5 cut(s)	146, 251, 332, 407, 1653
HpyCH4IV	ACGT	5 cut(s)	123, 1265, 1441, 1461, 1674
HpyF3I	CTNAG	2 cut(s)	42, 774
HpySE526I	ACGT	5 cut(s)	123, 1265, 1441, 1461, 1674
HspAI	GCGC	2 cut(s)	75, 185
KspI	CCGCGG	1 cut(s)	292
Kzo9I	GATC	5 cut(s)	199, 218, 964, 1483, 1664
LmnI	GCTCC	8 cut(s)	94, 109, 175, 270, 745, 824, 832, 1292
LweI	GCATC	3 cut(s)	142, 412, 1602
MabI	ACCWGGT	1 cut(s)	1033
MaeI	CTAG	4 cut(s)	741, 918, 1146, 1601
MaeII	ACGT	5 cut(s)	123, 1265, 1441, 1461, 1674
MaeIII	GTNAC	6 cut(s)	140, 455, 503, 1202, 1647, 1720
MalI	GATC	5 cut(s)	201, 220, 966, 1485, 1666
MbiI	CCGCTC	2 cut(s)	273, 1401
MboI	GATC	5 cut(s)	199, 218, 964, 1483, 1664
MboII	GAAGA	6 cut(s)	54, 374, 443, 744, 903, 1478
MflI	RGATCY	1 cut(s)	218
MhlI	GDGCHC	4 cut(s)	91, 106, 613, 829
MlsI	TGGCCA	1 cut(s)	1592
MluCI	AATT	8 cut(s)	31, 576, 779, 831, 1005, 1175, 1413, 1579
MluNI	TGGCCA	1 cut(s)	1592
MmeI	TCCRAC	2 cut(s)	1111, 1189
Mox20I	TGGCCA	1 cut(s)	1592
MroXI	GAANNNNTTC	3 cut(s)	435, 1179, 1734
MscI	TGGCCA	1 cut(s)	1592
MseI	TTAA	4 cut(s)	414, 513, 1395, 1569
MslI	CAYNNNNRTG	1 cut(s)	440
Msp20I	TGGCCA	1 cut(s)	1592
MspA1I	CMGCKG	5 cut(s)	41, 291, 562, 635, 844
MspI	CCGG	1 cut(s)	81
MspR9I	CCNGG	3 cut(s)	888, 1035, 1304
Mva1269I	GAATGC	1 cut(s)	975
MvaI	CCWGG	3 cut(s)	888, 1035, 1304
MvnI	CGCG	2 cut(s)	112, 291
NdeII	GATC	5 cut(s)	199, 218, 964, 1483, 1664
NlaIV	GGNNCC	3 cut(s)	162, 329, 1687
NmuCI	GTSAC	5 cut(s)	455, 503, 1202, 1647, 1720
NotI	GCGGCCGC	1 cut(s)	286
NspI	RCATGY	5 cut(s)	569, 609, 1027, 1226, 1472
NspV	TTCGAA	1 cut(s)	1728
PaeI	GCATGC	2 cut(s)	569, 1226
PagI	TCATGA	2 cut(s)	666, 1183
PaqCI	CACCTGC	1 cut(s)	175
PciI	ACATGT	1 cut(s)	1468
PcsI	WCGNNNNNNNCGW	1 cut(s)	1531
PctI	GAATGC	1 cut(s)	975
PdmI	GAANNNNTTC	3 cut(s)	435, 1179, 1734
PfeI	GAWTC	5 cut(s)	663, 1187, 1298, 1619, 1735
PflMI	CCANNNNNTGG	1 cut(s)	1171
PfoI	TCCNGGA	1 cut(s)	886
PmaCI	CACGTG	1 cut(s)	1442
PmlI	CACGTG	1 cut(s)	1442
Ppu21I	YACGTR	2 cut(s)	1442, 1675
PscI	ACATGT	1 cut(s)	1468
PshAI	GACNNNNGTC	1 cut(s)	249
Psp124BI	GAGCTC	1 cut(s)	829
Psp6I	CCWGG	3 cut(s)	886, 1033, 1302
PspCI	CACGTG	1 cut(s)	1442
PspFI	CCCAGC	2 cut(s)	1538, 1846
PspGI	CCWGG	3 cut(s)	886, 1033, 1302
PspN4I	GGNNCC	3 cut(s)	162, 329, 1687
PspPI	GGNCC	6 cut(s)	114, 160, 328, 673, 1213, 1233
PstI	CTGCAG	1 cut(s)	40
PstNI	CAGNNNCTG	2 cut(s)	844, 1090
PsuI	RGATCY	1 cut(s)	218
PvuII	CAGCTG	3 cut(s)	41, 635, 844
RsaI	GTAC	5 cut(s)	1115, 1150, 1243, 1528, 1635
RsaNI	GTAC	5 cut(s)	1114, 1149, 1242, 1527, 1634
RseI	CAYNNNNRTG	1 cut(s)	440
SacI	GAGCTC	1 cut(s)	829
SacII	CCGCGG	1 cut(s)	292
SaqAI	TTAA	4 cut(s)	414, 513, 1395, 1569
Sau3AI	GATC	5 cut(s)	199, 218, 964, 1483, 1664
Sau96I	GGNCC	6 cut(s)	114, 160, 328, 673, 1213, 1233
ScaI	AGTACT	1 cut(s)	1150
ScrFI	CCNGG	3 cut(s)	888, 1035, 1304
SduI	GDGCHC	4 cut(s)	91, 106, 613, 829
SexAI	ACCWGGT	1 cut(s)	1033
SfaNI	GCATC	3 cut(s)	142, 412, 1602
SfcI	CTRYAG	2 cut(s)	36, 1740
Sfr303I	CCGCGG	1 cut(s)	292
SfuI	TTCGAA	1 cut(s)	1728
SgrBI	CCGCGG	1 cut(s)	292
SinI	GGWCC	4 cut(s)	114, 160, 328, 1213
SmiMI	CAYNNNNRTG	1 cut(s)	440
SphI	GCATGC	2 cut(s)	569, 1226
Sse9I	AATT	8 cut(s)	31, 576, 779, 831, 1005, 1175, 1413, 1579
SspI	AATATT	2 cut(s)	523, 937
SspMI	CTAG	4 cut(s)	741, 918, 1146, 1601
SstI	GAGCTC	1 cut(s)	829
StyD4I	CCNGG	3 cut(s)	886, 1033, 1302
StyI	CCWWGG	1 cut(s)	583
TaaI	ACNGT	5 cut(s)	146, 251, 332, 407, 1653
TaiI	ACGT	5 cut(s)	126, 1268, 1444, 1464, 1677
TaqI	TCGA	4 cut(s)	202, 967, 1663, 1728
TaqII	GACCGA	1 cut(s)	243
TasI	AATT	8 cut(s)	31, 576, 779, 831, 1005, 1175, 1413, 1579
TatI	WGTACW	2 cut(s)	1113, 1148
TfiI	GAWTC	5 cut(s)	663, 1187, 1298, 1619, 1735
Tru1I	TTAA	4 cut(s)	414, 513, 1395, 1569
Tru9I	TTAA	4 cut(s)	414, 513, 1395, 1569
TscAI	CASTG	5 cut(s)	100, 412, 1066, 1316, 1593
TseFI	GTSAC	5 cut(s)	455, 503, 1202, 1647, 1720
Tsp45I	GTSAC	5 cut(s)	455, 503, 1202, 1647, 1720
TspGWI	ACGGA	1 cut(s)	96
TspRI	CASTG	5 cut(s)	100, 412, 1066, 1316, 1593
Van91I	CCANNNNNTGG	1 cut(s)	1171
VpaK11BI	GGWCC	4 cut(s)	114, 160, 328, 1213
XagI	CCTNNNNNAGG	2 cut(s)	956, 1032
XapI	RAATTY	3 cut(s)	31, 779, 1175
XceI	RCATGY	5 cut(s)	569, 609, 1027, 1226, 1472
XmnI	GAANNNNTTC	3 cut(s)	435, 1179, 1734
XspI	CTAG	4 cut(s)	741, 918, 1146, 1601
ZrmI	AGTACT	1 cut(s)	1150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.