RLG00000016349

Proteasome subunit beta type-8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000684
Physical Location & Seq
Forward (+)
5848457 .. 5849463
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016349

Sequence Viewer

Length: 639 bp
ATGGCTATAGGGACTACAAATATTGCATTCATGACTCGGACGGGGATAACCGCTGCTGTAGATTCCAGAACATGGCATGGCCAATTTGTAGACACAGATTTGGATATGAAGTTTTATACCTTATCAGAGAGATCAAATTTACATTGCACAGCCATGGGTCTTGTTCGTAGGTGGAATAATCTGTTCAACTATGTGAGCCAGAATGTCGAAATTAATAATGAAATACTTGACAACGAAGAATCCACAACAGCTTCAGTAGCAAAGGACTTTGTGCTAGAACACTTAAGAAACAATTGTCAGATTGAAGAAGCTGATCGAATTTATGGTACTCTTCTAGTTGGCTGGCCTCGAGACAAGCCTATGGAAATTCAGTCCATTCATATAGCAGGAAATCAACCTCATGTGGAAGGGATGAGTCCACCAGAAGAAGTACTAGAAGAACCTTCATTTTGGACATGTGGAGCGATATTGGGATCTGGCTATTCATATGCTTATCCAGTTATGATACGAGGCTATGATCCCGACATGTCAGATGACGACGCAGCTGACACAGCTTTAGAAGGTTTGTTTGTGGCATCTTTGAAAGATAAGTACACAGGAGGTCTTATGCTTGGTAATCTTTACTGCTCTAATACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000226 GO:0000502 GO:0002376 GO:0003674 GO:0003824 GO:0004175 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0005839 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007098 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010498 GO:0016043 GO:0016579 GO:0016787 GO:0019221 GO:0019222 GO:0019538 GO:0019774 GO:0019882 GO:0019941 GO:0022402 GO:0023052 GO:0030154 GO:0030162 GO:0030163 GO:0031023 GO:0031974 GO:0031981 GO:0032502 GO:0032991 GO:0034097 GO:0034340 GO:0036211 GO:0042221 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0043687 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0045444 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051246 GO:0051336 GO:0051603 GO:0051716 GO:0052547 GO:0052548 GO:0060255 GO:0060337 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070646 GO:0070647 GO:0070887 GO:0071310 GO:0071345 GO:0071357 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369 GO:1990111
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

213

Amino Acids

23.67

Weight (kDa)

4.5

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 72
AccI GTMKAC 1 cut(s) 90
AciI CCGC 1 cut(s) 51
AclWI GGATC 2 cut(s) 481, 512
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 3 cut(s) 136, 318, 366
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 3 cut(s) 328, 432, 593
AfiI CCNNNNNNNGG 1 cut(s) 72
AflII CTTAAG 1 cut(s) 283
AflIII ACRYGT 2 cut(s) 455, 525
AgsI TTSAA 3 cut(s) 187, 305, 583
AluBI AGCT 4 cut(s) 251, 311, 545, 554
AluI AGCT 4 cut(s) 251, 311, 545, 554
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 2 cut(s) 481, 512
Ama87I CYCGRG 1 cut(s) 348
AoxI GGCC 2 cut(s) 79, 344
ApeKI GCWGC 2 cut(s) 53, 542
ApoI RAATTY 3 cut(s) 136, 318, 366
AseI ATTAAT 1 cut(s) 213
AvaI CYCGRG 1 cut(s) 348
BalI TGGCCA 1 cut(s) 81
BbvI GCAGC 2 cut(s) 40, 554
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 3 cut(s) 275, 335, 434
BfmI CTRYAG 2 cut(s) 6, 57
BfrI CTTAAG 1 cut(s) 283
BisI GCNGC 2 cut(s) 54, 543
BlsI GCNGC 2 cut(s) 55, 544
BmcAI AGTACT 1 cut(s) 432
BmeT110I CYCGRG 1 cut(s) 348
BmsI GCATC 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 636
BsaJI CCNNGG 1 cut(s) 153
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 497
Bse3DI GCAATG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 417
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 497
BseXI GCAGC 2 cut(s) 40, 554
BshFI GGCC 2 cut(s) 81, 346
BsiHKCI CYCGRG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 345
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 2 cut(s) 81, 346
BsoBI CYCGRG 1 cut(s) 348
Bsp143I GATC 4 cut(s) 131, 313, 473, 517
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 1 cut(s) 51
BspANI GGCC 2 cut(s) 81, 346
BspHI TCATGA 1 cut(s) 30
BspPI GGATC 2 cut(s) 481, 512
BspTI CTTAAG 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 142
BsrI ACTGG 1 cut(s) 497
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 4 cut(s) 131, 313, 473, 517
BssT1I CCWWGG 1 cut(s) 153
Bst6I CTCTTC 1 cut(s) 336
BstAFI CTTAAG 1 cut(s) 283
BstBAI YACGTR 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 344
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 417
BstKTI GATC 4 cut(s) 134, 316, 476, 520
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 4 cut(s) 131, 313, 473, 517
BstMWI GCNNNNNNNGC 2 cut(s) 257, 551
BstNSI RCATGY 2 cut(s) 459, 529
BstSFI CTRYAG 2 cut(s) 6, 57
BstV1I GCAGC 2 cut(s) 40, 554
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BsuRI GGCC 2 cut(s) 81, 346
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 417
Cac8I GCNNGC 1 cut(s) 344
CciI TCATGA 1 cut(s) 30
CseI GACGC 1 cut(s) 548
Csp6I GTAC 3 cut(s) 327, 431, 592
CviAII CATG 7 cut(s) 31, 72, 77, 154, 401, 456, 526
CviQI GTAC 3 cut(s) 327, 431, 592
DpnI GATC 4 cut(s) 133, 315, 475, 519
DpnII GATC 4 cut(s) 131, 313, 473, 517
EaeI YGGCCR 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 336
EarI CTCTTC 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 348
EcoT14I CCWWGG 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 153
FaeI CATG 7 cut(s) 34, 75, 80, 157, 404, 459, 529
FaqI GGGAC 1 cut(s) 25
FatI CATG 7 cut(s) 30, 71, 76, 153, 400, 455, 525
FauNDI CATATG 1 cut(s) 487
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 54, 543
FokI GGATG 1 cut(s) 424
Fsp4HI GCNGC 2 cut(s) 54, 543
FspBI CTAG 3 cut(s) 275, 335, 434
GluI GCNGC 2 cut(s) 54, 543
HaeIII GGCC 2 cut(s) 81, 346
HgaI GACGC 1 cut(s) 548
Hin1II CATG 7 cut(s) 34, 75, 80, 157, 404, 459, 529
HinfI GANTC 4 cut(s) 34, 62, 239, 415
Hpy166II GTNNAC 3 cut(s) 91, 419, 594
Hpy188I TCNGA 4 cut(s) 39, 127, 300, 532
Hpy188III TCNNGA 4 cut(s) 31, 66, 350, 521
Hpy8I GTNNAC 3 cut(s) 91, 419, 594
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 3 cut(s) 401, 453, 554
HpyCH4IV ACGT 1 cut(s) 635
HpyCH4V TGCA 2 cut(s) 26, 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 551
HpySE526I ACGT 1 cut(s) 635
Hsp92II CATG 7 cut(s) 34, 75, 80, 157, 404, 459, 529
Kzo9I GATC 4 cut(s) 131, 313, 473, 517
LmnI GCTCC 1 cut(s) 461
LpnPI CCDG 8 cut(s) 79, 212, 328, 372, 435, 462, 510, 582
Lsp1109I GCAGC 2 cut(s) 40, 554
LweI GCATC 1 cut(s) 584
MaeI CTAG 3 cut(s) 275, 335, 434
MaeII ACGT 1 cut(s) 635
MalI GATC 4 cut(s) 133, 315, 475, 519
MboI GATC 4 cut(s) 131, 313, 473, 517
MboII GAAGA 5 cut(s) 248, 317, 323, 437, 449
MfeI CAATTG 1 cut(s) 292
MflI RGATCY 1 cut(s) 473
MlsI TGGCCA 1 cut(s) 81
MluCI AATT 6 cut(s) 83, 136, 210, 292, 318, 366
MluNI TGGCCA 1 cut(s) 81
MlyI GAGTC 2 cut(s) 28, 424
MnlI CCTC 4 cut(s) 357, 408, 503, 593
Mox20I TGGCCA 1 cut(s) 81
MscI TGGCCA 1 cut(s) 81
MseI TTAA 2 cut(s) 213, 284
MslI CAYNNNNRTG 1 cut(s) 152
Msp20I TGGCCA 1 cut(s) 81
MspA1I CMGCKG 2 cut(s) 53, 545
MspCI CTTAAG 1 cut(s) 283
MunI CAATTG 1 cut(s) 292
Mva1269I GAATGC 1 cut(s) 26
MwoI GCNNNNNNNGC 2 cut(s) 257, 551
NcoI CCATGG 1 cut(s) 153
NdeI CATATG 1 cut(s) 487
NdeII GATC 4 cut(s) 131, 313, 473, 517
NlaIII CATG 7 cut(s) 34, 75, 80, 157, 404, 459, 529
NspI RCATGY 2 cut(s) 459, 529
PaeR7I CTCGAG 1 cut(s) 348
PagI TCATGA 1 cut(s) 30
PciI ACATGT 2 cut(s) 455, 525
PctI GAATGC 1 cut(s) 26
PfeI GAWTC 2 cut(s) 62, 239
PflMI CCANNNNNTGG 1 cut(s) 72
PkrI GCNGC 2 cut(s) 55, 544
PleI GAGTC 2 cut(s) 28, 423
PpsI GAGTC 2 cut(s) 28, 423
Ppu21I YACGTR 1 cut(s) 636
PscI ACATGT 2 cut(s) 455, 525
PshBI ATTAAT 1 cut(s) 213
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 3 cut(s) 328, 432, 593
RsaNI GTAC 3 cut(s) 327, 431, 592
RseI CAYNNNNRTG 1 cut(s) 152
SaqAI TTAA 2 cut(s) 213, 284
SatI GCNGC 2 cut(s) 54, 543
Sau3AI GATC 4 cut(s) 131, 313, 473, 517
ScaI AGTACT 1 cut(s) 432
SchI GAGTC 2 cut(s) 28, 424
SfaNI GCATC 1 cut(s) 584
SfcI CTRYAG 2 cut(s) 6, 57
Sfr274I CTCGAG 1 cut(s) 348
SlaI CTCGAG 1 cut(s) 348
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 2 cut(s) 283, 348
SmoI CTYRAG 2 cut(s) 283, 348
Sse9I AATT 6 cut(s) 83, 136, 210, 292, 318, 366
SsiI CCGC 1 cut(s) 51
SspI AATATT 1 cut(s) 22
SspMI CTAG 3 cut(s) 275, 335, 434
StyI CCWWGG 1 cut(s) 153
TaiI ACGT 1 cut(s) 638
TaqI TCGA 3 cut(s) 207, 316, 349
TasI AATT 6 cut(s) 83, 136, 210, 292, 318, 366
TatI WGTACW 2 cut(s) 430, 591
TfiI GAWTC 2 cut(s) 62, 239
Tru1I TTAA 2 cut(s) 213, 284
Tru9I TTAA 2 cut(s) 213, 284
TseI GCWGC 2 cut(s) 53, 542
TspDTI ATGAA 6 cut(s) 19, 122, 234, 368, 435, 474
Van91I CCANNNNNTGG 1 cut(s) 72
Vha464I CTTAAG 1 cut(s) 283
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 3 cut(s) 136, 318, 366
XceI RCATGY 2 cut(s) 459, 529
XhoI CTCGAG 1 cut(s) 348
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 3 cut(s) 275, 335, 434
ZrmI AGTACT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.