DEAD-box ATP-dependent RNA helicase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 860 |
| AccBSI | CCGCTC | 1 cut(s) | 432 |
| AccI | GTMKAC | 1 cut(s) | 179 |
| AccII | CGCG | 3 cut(s) | 79, 243, 548 |
| AccIII | TCCGGA | 1 cut(s) | 602 |
| AcoI | YGGCCR | 5 cut(s) | 160, 223, 274, 564, 568 |
| AcsI | RAATTY | 3 cut(s) | 341, 394, 1840 |
| AcuI | CTGAAG | 1 cut(s) | 1537 |
| AcyI | GRCGYC | 2 cut(s) | 299, 530 |
| AfaI | GTAC | 5 cut(s) | 188, 294, 518, 1879, 1912 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 873, 1019, 1579 |
| AflII | CTTAAG | 1 cut(s) | 1205 |
| AflIII | ACRYGT | 1 cut(s) | 330 |
| AgsI | TTSAA | 5 cut(s) | 493, 896, 974, 998, 1381 |
| AjiI | CACGTC | 3 cut(s) | 331, 442, 1520 |
| AjnI | CCWGG | 3 cut(s) | 871, 1018, 1304 |
| AluBI | AGCT | 9 cut(s) | 153, 352, 707, 758, 829, 1240, 1367, 1749, 1929 |
| AluI | AGCT | 9 cut(s) | 153, 352, 707, 758, 829, 1240, 1367, 1749, 1929 |
| Alw21I | GWGCWC | 3 cut(s) | 461, 592, 709 |
| Alw26I | GTCTC | 4 cut(s) | 672, 723, 1259, 1662 |
| Ama87I | CYCGRG | 1 cut(s) | 1924 |
| Aor13HI | TCCGGA | 1 cut(s) | 602 |
| AoxI | GGCC | 8 cut(s) | 24, 160, 223, 274, 305, 318, 564, 568 |
| ApeKI | GCWGC | 6 cut(s) | 349, 826, 1079, 1746, 1805, 1862 |
| ApoI | RAATTY | 3 cut(s) | 341, 394, 1840 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 953, 985 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1313 |
| AspLEI | GCGC | 4 cut(s) | 81, 543, 814, 879 |
| AspS9I | GGNCC | 4 cut(s) | 24, 233, 319, 600 |
| AsuC2I | CCSGG | 5 cut(s) | 49, 237, 337, 572, 598 |
| AsuHPI | GGTGA | 3 cut(s) | 46, 1349, 1904 |
| AvaI | CYCGRG | 1 cut(s) | 1924 |
| AvaII | GGWCC | 2 cut(s) | 233, 600 |
| BanII | GRGCYC | 1 cut(s) | 709 |
| BauI | CACGAG | 2 cut(s) | 903, 1425 |
| BbsI | GAAGAC | 1 cut(s) | 1193 |
| Bbv12I | GWGCWC | 3 cut(s) | 461, 592, 709 |
| BbvI | GCAGC | 6 cut(s) | 361, 838, 1066, 1758, 1792, 1849 |
| BccI | CCATC | 6 cut(s) | 10, 131, 574, 677, 811, 1895 |
| BceAI | ACGGC | 3 cut(s) | 147, 175, 627 |
| BciT130I | CCWGG | 3 cut(s) | 873, 1020, 1306 |
| BclI | TGATCA | 1 cut(s) | 921 |
| BcnI | CCSGG | 5 cut(s) | 49, 237, 337, 572, 598 |
| BcoDI | GTCTC | 4 cut(s) | 672, 723, 1259, 1662 |
| BfaI | CTAG | 4 cut(s) | 171, 738, 860, 938 |
| BfmI | CTRYAG | 1 cut(s) | 1080 |
| BfoI | RGCGCY | 3 cut(s) | 544, 815, 880 |
| BfrI | CTTAAG | 1 cut(s) | 1205 |
| Bme1390I | CCNGG | 8 cut(s) | 49, 237, 337, 572, 598, 873, 1020, 1306 |
| Bme18I | GGWCC | 2 cut(s) | 233, 600 |
| BmeT110I | CYCGRG | 1 cut(s) | 1924 |
| BmgBI | CACGTC | 3 cut(s) | 331, 442, 1520 |
| BmgT120I | GGNCC | 4 cut(s) | 24, 233, 319, 600 |
| BmiI | GGNNCC | 4 cut(s) | 450, 601, 1620, 1921 |
| BmrFI | CCNGG | 8 cut(s) | 49, 237, 337, 572, 598, 873, 1020, 1306 |
| BmsI | GCATC | 2 cut(s) | 112, 391 |
| BpiI | GAAGAC | 1 cut(s) | 1193 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 897, 929 |
| BpmI | CTGGAG | 1 cut(s) | 855 |
| BpuEI | CTTGAG | 2 cut(s) | 899, 1076 |
| BpuMI | CCSGG | 5 cut(s) | 49, 237, 337, 572, 598 |
| BsaAI | YACGTR | 2 cut(s) | 94, 520 |
| BsaHI | GRCGYC | 2 cut(s) | 299, 530 |
| BsaI | GGTCTC | 3 cut(s) | 672, 1259, 1662 |
| BsaJI | CCNNGG | 6 cut(s) | 163, 302, 561, 872, 1018, 1251 |
| BsaWI | WCCGGW | 2 cut(s) | 418, 602 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 873, 1019, 1579 |
| Bse118I | RCCGGY | 3 cut(s) | 445, 532, 566 |
| Bse1I | ACTGG | 4 cut(s) | 1301, 1407, 1519, 1590 |
| Bse3DI | GCAATG | 1 cut(s) | 388 |
| BseAI | TCCGGA | 1 cut(s) | 602 |
| BseBI | CCWGG | 3 cut(s) | 873, 1020, 1306 |
| BseDI | CCNNGG | 6 cut(s) | 163, 302, 561, 872, 1018, 1251 |
| BseGI | GGATG | 6 cut(s) | 688, 855, 960, 1420, 1713, 1858 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 873, 1019, 1579 |
| BseMI | GCAATG | 1 cut(s) | 388 |
| BseMII | CTCAG | 5 cut(s) | 197, 605, 683, 750, 1244 |
| BseNI | ACTGG | 4 cut(s) | 1301, 1407, 1519, 1590 |
| BseRI | GAGGAG | 1 cut(s) | 270 |
| BseX3I | CGGCCG | 3 cut(s) | 160, 223, 568 |
| BseXI | GCAGC | 6 cut(s) | 361, 838, 1066, 1758, 1792, 1849 |
| BseYI | CCCAGC | 1 cut(s) | 1318 |
| Bsh1236I | CGCG | 3 cut(s) | 79, 243, 548 |
| Bsh1285I | CGRYCG | 3 cut(s) | 163, 226, 571 |
| BshFI | GGCC | 8 cut(s) | 26, 162, 225, 276, 307, 320, 566, 570 |
| BsiEI | CGRYCG | 3 cut(s) | 163, 226, 571 |
| BsiHKAI | GWGCWC | 3 cut(s) | 461, 592, 709 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1924 |
| BslFI | GGGAC | 2 cut(s) | 587, 1232 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 873, 1019, 1579 |
| BsmAI | GTCTC | 4 cut(s) | 672, 723, 1259, 1662 |
| BsmFI | GGGAC | 2 cut(s) | 587, 1232 |
| BsnI | GGCC | 8 cut(s) | 26, 162, 225, 276, 307, 320, 566, 570 |
| Bso31I | GGTCTC | 3 cut(s) | 672, 1259, 1662 |
| BsoBI | CYCGRG | 1 cut(s) | 1924 |
| Bsp1286I | GDGCHC | 3 cut(s) | 461, 592, 709 |
| Bsp13I | TCCGGA | 1 cut(s) | 602 |
| Bsp143I | GATC | 2 cut(s) | 921, 1138 |
| Bsp19I | CCATGG | 1 cut(s) | 561 |
| Bsp68I | TCGCGA | 1 cut(s) | 243 |
| BspANI | GGCC | 8 cut(s) | 26, 162, 225, 276, 307, 320, 566, 570 |
| BspCNI | CTCAG | 5 cut(s) | 196, 604, 682, 751, 1243 |
| BspEI | TCCGGA | 1 cut(s) | 602 |
| BspFNI | CGCG | 3 cut(s) | 79, 243, 548 |
| BspHI | TCATGA | 1 cut(s) | 325 |
| BspLI | GGNNCC | 4 cut(s) | 450, 601, 1620, 1921 |
| BspMAI | CTGCAG | 1 cut(s) | 1084 |
| BspTI | CTTAAG | 1 cut(s) | 1205 |
| BspTNI | GGTCTC | 3 cut(s) | 672, 1259, 1662 |
| BsrBI | CCGCTC | 1 cut(s) | 432 |
| BsrDI | GCAATG | 1 cut(s) | 388 |
| BsrFI | RCCGGY | 3 cut(s) | 445, 532, 566 |
| BsrI | ACTGG | 4 cut(s) | 1301, 1407, 1519, 1590 |
| BssAI | RCCGGY | 3 cut(s) | 445, 532, 566 |
| BssECI | CCNNGG | 6 cut(s) | 163, 302, 561, 872, 1018, 1251 |
| BssMI | GATC | 2 cut(s) | 921, 1138 |
| BssNI | GRCGYC | 2 cut(s) | 299, 530 |
| BssSI | CACGAG | 2 cut(s) | 903, 1425 |
| BssT1I | CCWWGG | 2 cut(s) | 561, 1251 |
| Bst2BI | CACGAG | 2 cut(s) | 903, 1425 |
| Bst2UI | CCWGG | 3 cut(s) | 873, 1020, 1306 |
| Bst4CI | ACNGT | 5 cut(s) | 191, 233, 694, 1114, 1650 |
| BstACI | GRCGYC | 2 cut(s) | 299, 530 |
| BstAFI | CTTAAG | 1 cut(s) | 1205 |
| BstBAI | YACGTR | 2 cut(s) | 94, 520 |
| BstC8I | GCNNGC | 7 cut(s) | 430, 447, 457, 568, 1077, 1242, 1860 |
| BstDEI | CTNAG | 7 cut(s) | 183, 591, 651, 669, 759, 880, 1230 |
| BstDSI | CCRYGG | 2 cut(s) | 163, 561 |
| BstEII | GGTNACC | 1 cut(s) | 34 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1017, 1577 |
| BstF5I | GGATG | 6 cut(s) | 688, 855, 960, 1420, 1713, 1858 |
| BstFNI | CGCG | 3 cut(s) | 79, 243, 548 |
| BstH2I | RGCGCY | 3 cut(s) | 544, 815, 880 |
| BstHHI | GCGC | 4 cut(s) | 81, 543, 814, 879 |
| BstKTI | GATC | 2 cut(s) | 924, 1141 |
| BstMAI | GTCTC | 4 cut(s) | 672, 723, 1259, 1662 |
| BstMBI | GATC | 2 cut(s) | 921, 1138 |
| BstMCI | CGRYCG | 3 cut(s) | 163, 226, 571 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 117, 126, 159, 538, 791, 1913 |
| BstNI | CCWGG | 3 cut(s) | 873, 1020, 1306 |
| BstNSI | RCATGY | 1 cut(s) | 1012 |
| BstPI | GGTNACC | 1 cut(s) | 34 |
| BstSCI | CCNGG | 8 cut(s) | 47, 235, 335, 570, 596, 871, 1018, 1304 |
| BstSFI | CTRYAG | 1 cut(s) | 1080 |
| BstUI | CGCG | 3 cut(s) | 79, 243, 548 |
| BstV1I | GCAGC | 6 cut(s) | 361, 838, 1066, 1758, 1792, 1849 |
| BstV2I | GAAGAC | 1 cut(s) | 1193 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1685 |
| BstZI | CGGCCG | 3 cut(s) | 160, 223, 568 |
| BsuRI | GGCC | 8 cut(s) | 26, 162, 225, 276, 307, 320, 566, 570 |
| BtgI | CCRYGG | 2 cut(s) | 163, 561 |
| BtgZI | GCGATG | 1 cut(s) | 150 |
| BtrI | CACGTC | 3 cut(s) | 331, 442, 1520 |
| BtsCI | GGATG | 6 cut(s) | 688, 855, 960, 1420, 1713, 1858 |
| BtsI | GCAGTG | 1 cut(s) | 1034 |
| BtsIMutI | CAGTG | 6 cut(s) | 643, 1034, 1051, 1134, 1561, 1583 |
| BtuMI | TCGCGA | 1 cut(s) | 243 |
| Cac8I | GCNNGC | 7 cut(s) | 430, 447, 457, 568, 1077, 1242, 1860 |
| CciI | TCATGA | 1 cut(s) | 325 |
| CfoI | GCGC | 4 cut(s) | 81, 543, 814, 879 |
| Cfr10I | RCCGGY | 3 cut(s) | 445, 532, 566 |
| Cfr13I | GGNCC | 4 cut(s) | 24, 233, 319, 600 |
| CpoI | CGGWCCG | 1 cut(s) | 233 |
| CseI | GACGC | 2 cut(s) | 307, 538 |
| Csp6I | GTAC | 5 cut(s) | 187, 293, 517, 1878, 1911 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1386, 1421 |
| CspI | CGGWCCG | 1 cut(s) | 233 |
| CviQI | GTAC | 5 cut(s) | 187, 293, 517, 1878, 1911 |
| DdeI | CTNAG | 7 cut(s) | 183, 591, 651, 669, 759, 880, 1230 |
| DpnI | GATC | 2 cut(s) | 923, 1140 |
| DpnII | GATC | 2 cut(s) | 921, 1138 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 860 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 860 |
| EaeI | YGGCCR | 5 cut(s) | 160, 223, 274, 564, 568 |
| EagI | CGGCCG | 3 cut(s) | 160, 223, 568 |
| EciI | GGCGGA | 1 cut(s) | 34 |
| Ecl136II | GAGCTC | 1 cut(s) | 707 |
| EclXI | CGGCCG | 3 cut(s) | 160, 223, 568 |
| Eco130I | CCWWGG | 2 cut(s) | 561, 1251 |
| Eco24I | GRGCYC | 1 cut(s) | 709 |
| Eco31I | GGTCTC | 3 cut(s) | 672, 1259, 1662 |
| Eco32I | GATATC | 1 cut(s) | 1438 |
| Eco47I | GGWCC | 2 cut(s) | 233, 600 |
| Eco52I | CGGCCG | 3 cut(s) | 160, 223, 568 |
| Eco53kI | GAGCTC | 1 cut(s) | 707 |
| Eco57I | CTGAAG | 1 cut(s) | 1537 |
| Eco88I | CYCGRG | 1 cut(s) | 1924 |
| Eco91I | GGTNACC | 1 cut(s) | 34 |
| EcoICRI | GAGCTC | 1 cut(s) | 707 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1017, 1577 |
| EcoO109I | RGGNCCY | 1 cut(s) | 319 |
| EcoO65I | GGTNACC | 1 cut(s) | 34 |
| EcoRI | GAATTC | 1 cut(s) | 1840 |
| EcoRII | CCWGG | 3 cut(s) | 871, 1018, 1304 |
| EcoRV | GATATC | 1 cut(s) | 1438 |
| EcoT14I | CCWWGG | 2 cut(s) | 561, 1251 |
| EcoT38I | GRGCYC | 1 cut(s) | 709 |
| ErhI | CCWWGG | 2 cut(s) | 561, 1251 |
| FaqI | GGGAC | 2 cut(s) | 587, 1232 |
| FauI | CCCGC | 2 cut(s) | 81, 1065 |
| FauNDI | CATATG | 1 cut(s) | 1801 |
| FbaI | TGATCA | 1 cut(s) | 921 |
| FblI | GTMKAC | 1 cut(s) | 179 |
| FokI | GGATG | 6 cut(s) | 695, 842, 967, 1427, 1700, 1865 |
| FriOI | GRGCYC | 1 cut(s) | 709 |
| FseI | GGCCGGCC | 1 cut(s) | 570 |
| FspBI | CTAG | 4 cut(s) | 171, 738, 860, 938 |
| GlaI | GCGC | 4 cut(s) | 80, 542, 813, 878 |
| GsaI | CCCAGC | 1 cut(s) | 1322 |
| GsuI | CTGGAG | 1 cut(s) | 855 |
| HaeII | RGCGCY | 3 cut(s) | 544, 815, 880 |
| HaeIII | GGCC | 8 cut(s) | 26, 162, 225, 276, 307, 320, 566, 570 |
| HgaI | GACGC | 2 cut(s) | 307, 538 |
| HhaI | GCGC | 4 cut(s) | 81, 543, 814, 879 |
| Hin1I | GRCGYC | 2 cut(s) | 299, 530 |
| Hin6I | GCGC | 4 cut(s) | 79, 541, 812, 877 |
| HinP1I | GCGC | 4 cut(s) | 79, 541, 812, 877 |
| HincII | GTYRAC | 3 cut(s) | 180, 648, 853 |
| HindII | GTYRAC | 3 cut(s) | 180, 648, 853 |
| HindIII | AAGCTT | 1 cut(s) | 1238 |
| HinfI | GANTC | 9 cut(s) | 4, 41, 176, 181, 280, 550, 1314, 1604, 1763 |
| HphI | GGTGA | 3 cut(s) | 46, 1349, 1904 |
| Hpy166II | GTNNAC | 5 cut(s) | 180, 648, 853, 886, 1498 |
| Hpy188I | TCNGA | 9 cut(s) | 9, 555, 594, 672, 723, 744, 777, 1110, 1512 |
| Hpy188III | TCNNGA | 9 cut(s) | 242, 326, 505, 603, 833, 903, 916, 1093, 1425 |
| Hpy8I | GTNNAC | 5 cut(s) | 180, 648, 853, 886, 1498 |
| Hpy99I | CGWCG | 3 cut(s) | 248, 532, 727 |
| HpyAV | CCTTC | 2 cut(s) | 332, 1594 |
| HpyCH4III | ACNGT | 5 cut(s) | 191, 233, 694, 1114, 1650 |
| HpyCH4IV | ACGT | 5 cut(s) | 93, 330, 441, 519, 1519 |
| HpyCH4V | TGCA | 6 cut(s) | 1025, 1049, 1082, 1348, 1541, 1577 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 117, 126, 159, 538, 791, 1913 |
| HpyF3I | CTNAG | 7 cut(s) | 183, 591, 651, 669, 759, 880, 1230 |
| HpySE526I | ACGT | 5 cut(s) | 93, 330, 441, 519, 1519 |
| Hsp92I | GRCGYC | 2 cut(s) | 299, 530 |
| HspAI | GCGC | 4 cut(s) | 79, 541, 812, 877 |
| Kpn2I | TCCGGA | 1 cut(s) | 602 |
| KroI | GCCGGC | 2 cut(s) | 445, 566 |
| KroNI | GCCGGC | 2 cut(s) | 447, 568 |
| Ksp22I | TGATCA | 1 cut(s) | 921 |
| Kzo9I | GATC | 2 cut(s) | 921, 1138 |
| LmnI | GCTCC | 4 cut(s) | 158, 454, 809, 1277 |
| Lsp1109I | GCAGC | 6 cut(s) | 361, 838, 1066, 1758, 1792, 1849 |
| LweI | GCATC | 2 cut(s) | 112, 391 |
| MaeI | CTAG | 4 cut(s) | 171, 738, 860, 938 |
| MaeII | ACGT | 5 cut(s) | 93, 330, 441, 519, 1519 |
| MaeIII | GTNAC | 6 cut(s) | 34, 146, 209, 900, 1337, 1694 |
| MalI | GATC | 2 cut(s) | 923, 1140 |
| MbiI | CCGCTC | 1 cut(s) | 432 |
| MboI | GATC | 2 cut(s) | 921, 1138 |
| MboII | GAAGA | 7 cut(s) | 25, 98, 1000, 1193, 1543, 1758, 1836 |
| MfeI | CAATTG | 1 cut(s) | 1286 |
| MhlI | GDGCHC | 3 cut(s) | 461, 592, 709 |
| MluCI | AATT | 9 cut(s) | 341, 394, 1286, 1462, 1624, 1732, 1831, 1840, 1936 |
| MlyI | GAGTC | 5 cut(s) | 13, 50, 175, 185, 289 |
| MmeI | TCCRAC | 4 cut(s) | 668, 746, 939, 1490 |
| MroI | TCCGGA | 1 cut(s) | 602 |
| MroNI | GCCGGC | 2 cut(s) | 445, 566 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1313 |
| MseI | TTAA | 4 cut(s) | 1206, 1247, 1595, 1632 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1194 |
| MspA1I | CMGCKG | 1 cut(s) | 352 |
| MspCI | CTTAAG | 1 cut(s) | 1205 |
| MspR9I | CCNGG | 8 cut(s) | 49, 237, 337, 572, 598, 873, 1020, 1306 |
| MunI | CAATTG | 1 cut(s) | 1286 |
| MvaI | CCWGG | 3 cut(s) | 873, 1020, 1306 |
| MvnI | CGCG | 3 cut(s) | 79, 243, 548 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 117, 126, 159, 538, 791, 1913 |
| NaeI | GCCGGC | 2 cut(s) | 447, 568 |
| NciI | CCSGG | 5 cut(s) | 49, 237, 337, 572, 598 |
| NcoI | CCATGG | 1 cut(s) | 561 |
| NdeI | CATATG | 1 cut(s) | 1801 |
| NdeII | GATC | 2 cut(s) | 921, 1138 |
| NgoMIV | GCCGGC | 2 cut(s) | 445, 566 |
| NlaIV | GGNNCC | 4 cut(s) | 450, 601, 1620, 1921 |
| NmeAIII | GCCGAG | 1 cut(s) | 327 |
| NmuCI | GTSAC | 3 cut(s) | 34, 900, 1337 |
| NruI | TCGCGA | 1 cut(s) | 243 |
| NspI | RCATGY | 1 cut(s) | 1012 |
| PaeR7I | CTCGAG | 1 cut(s) | 1924 |
| PagI | TCATGA | 1 cut(s) | 325 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 305, 405, 1516 |
| PdiI | GCCGGC | 2 cut(s) | 447, 568 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1313 |
| PfeI | GAWTC | 4 cut(s) | 550, 1314, 1604, 1763 |
| PflFI | GACNNNGTC | 2 cut(s) | 40, 598 |
| PfoI | TCCNGGA | 2 cut(s) | 335, 1304 |
| PleI | GAGTC | 5 cut(s) | 12, 49, 175, 184, 288 |
| PpsI | GAGTC | 5 cut(s) | 12, 49, 175, 184, 288 |
| Ppu21I | YACGTR | 2 cut(s) | 94, 520 |
| Psp124BI | GAGCTC | 1 cut(s) | 709 |
| Psp6I | CCWGG | 3 cut(s) | 871, 1018, 1304 |
| PspEI | GGTNACC | 1 cut(s) | 34 |
| PspFI | CCCAGC | 1 cut(s) | 1318 |
| PspGI | CCWGG | 3 cut(s) | 871, 1018, 1304 |
| PspN4I | GGNNCC | 4 cut(s) | 450, 601, 1620, 1921 |
| PspPI | GGNCC | 4 cut(s) | 24, 233, 319, 600 |
| PspXI | VCTCGAGB | 1 cut(s) | 1924 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1903, 1935 |
| PstI | CTGCAG | 1 cut(s) | 1084 |
| PsyI | GACNNNGTC | 2 cut(s) | 40, 598 |
| PvuII | CAGCTG | 1 cut(s) | 352 |
| RigI | GGCCGGCC | 1 cut(s) | 570 |
| RruI | TCGCGA | 1 cut(s) | 243 |
| RsaI | GTAC | 5 cut(s) | 188, 294, 518, 1879, 1912 |
| RsaNI | GTAC | 5 cut(s) | 187, 293, 517, 1878, 1911 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1194 |
| Rsr2I | CGGWCCG | 1 cut(s) | 233 |
| RsrII | CGGWCCG | 1 cut(s) | 233 |
| SacI | GAGCTC | 1 cut(s) | 709 |
| SalI | GTCGAC | 1 cut(s) | 178 |
| SaqAI | TTAA | 4 cut(s) | 1206, 1247, 1595, 1632 |
| Sau3AI | GATC | 2 cut(s) | 921, 1138 |
| Sau96I | GGNCC | 4 cut(s) | 24, 233, 319, 600 |
| SchI | GAGTC | 5 cut(s) | 13, 50, 175, 185, 289 |
| ScrFI | CCNGG | 8 cut(s) | 49, 237, 337, 572, 598, 873, 1020, 1306 |
| SduI | GDGCHC | 3 cut(s) | 461, 592, 709 |
| SfaNI | GCATC | 2 cut(s) | 112, 391 |
| SfcI | CTRYAG | 1 cut(s) | 1080 |
| Sfr274I | CTCGAG | 1 cut(s) | 1924 |
| SinI | GGWCC | 2 cut(s) | 233, 600 |
| SlaI | CTCGAG | 1 cut(s) | 1924 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1194 |
| SmlI | CTYRAG | 4 cut(s) | 914, 1091, 1205, 1924 |
| SmoI | CTYRAG | 4 cut(s) | 914, 1091, 1205, 1924 |
| Sse9I | AATT | 9 cut(s) | 341, 394, 1286, 1462, 1624, 1732, 1831, 1840, 1936 |
| SspI | AATATT | 1 cut(s) | 1948 |
| SspMI | CTAG | 4 cut(s) | 171, 738, 860, 938 |
| SstI | GAGCTC | 1 cut(s) | 709 |
| StyD4I | CCNGG | 8 cut(s) | 47, 235, 335, 570, 596, 871, 1018, 1304 |
| StyI | CCWWGG | 2 cut(s) | 561, 1251 |
| TaaI | ACNGT | 5 cut(s) | 191, 233, 694, 1114, 1650 |
| TaiI | ACGT | 5 cut(s) | 96, 333, 444, 522, 1522 |
| TaqI | TCGA | 5 cut(s) | 179, 283, 399, 1607, 1925 |
| TaqII | GACCGA | 1 cut(s) | 53 |
| TasI | AATT | 9 cut(s) | 341, 394, 1286, 1462, 1624, 1732, 1831, 1840, 1936 |
| TauI | GCSGC | 5 cut(s) | 79, 123, 225, 435, 617 |
| TfiI | GAWTC | 4 cut(s) | 550, 1314, 1604, 1763 |
| Tru1I | TTAA | 4 cut(s) | 1206, 1247, 1595, 1632 |
| Tru9I | TTAA | 4 cut(s) | 1206, 1247, 1595, 1632 |
| TscAI | CASTG | 6 cut(s) | 643, 1041, 1051, 1141, 1561, 1590 |
| TseFI | GTSAC | 3 cut(s) | 34, 900, 1337 |
| TseI | GCWGC | 6 cut(s) | 349, 826, 1079, 1746, 1805, 1862 |
| Tsp45I | GTSAC | 3 cut(s) | 34, 900, 1337 |
| TspDTI | ATGAA | 7 cut(s) | 314, 1077, 1239, 1613, 1788, 1803, 1956 |
| TspGWI | ACGGA | 3 cut(s) | 261, 1427, 1706 |
| TspRI | CASTG | 6 cut(s) | 643, 1041, 1051, 1141, 1561, 1590 |
| Tth111I | GACNNNGTC | 2 cut(s) | 40, 598 |
| Vha464I | CTTAAG | 1 cut(s) | 1205 |
| VpaK11BI | GGWCC | 2 cut(s) | 233, 600 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1017, 1577 |
| XapI | RAATTY | 3 cut(s) | 341, 394, 1840 |
| XceI | RCATGY | 1 cut(s) | 1012 |
| XhoI | CTCGAG | 1 cut(s) | 1924 |
| XmiI | GTMKAC | 1 cut(s) | 179 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1313 |
| XspI | CTAG | 4 cut(s) | 171, 738, 860, 938 |