pycom02g02800

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000684
Physical Location & Seq
Reverse (-)
1763852 .. 1766716
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02800.4

Sequence Viewer

Length: 1872 bp
ATGACAATGCCATGGTCCGATTCCTTCACCGAGTCAACTCGGAACCCGACTCGCCGACCTCTGTCCAAATACGTACCTCGGTACCTCCGAAACAGTGGCGACGGCGTGGGGTCGTACGGTTACGGCTACGCGCCCCCAACCGAGTCGGCCCAGTACGGCTTTCGGCAAAGCTACGCTGGTGGTCCCGGAAGGCAAGCCGGCCGGAACGGTCCGGGTCGCGGCGGCGGAGGACGAGGTCGCGGACGTGGTGGCGGTCGCTTTTGGGCTCAACCGCCCCCAAACCCGACCCCAGACCCAAACCCGTTTGATAATGTCCCTGAGGAATTTGATCAGCTCAAAGTCACTGAGGACGAAGGCGATGGAAATGGCGGTATCAATTTCGACGCCTACGAAGACATCCCGGTGGAGGCGAGCGGCGAGGACATTCCGCCGCCGGTGGACACGTTTGTCGAGATCGATTTGGCTGAATGCTTGAACGAAAACATCAAGCGGTGCAAGTACGTGAAGCCCACGCCGATTCAGCGGCACGCTATTCCGATAGCCATGGCTGGTCGCGACTTGATGGCCTGTGCTCAGACTGGGTCCGGCAAAACGGCTGCGTTTTGCTTCCCCATTATCAGTGGTGTCTTGAACAACGGTCTGGGGTTGTCGCCGGCTCGCGGGGTGGATCGCGGCGTGTGCCCCACCGCCCTCATTTTGTCTCCGACGAGAGAGCTGGCTGGTCAGATTCATGAGGAAGCTAAGAAGTTTGGTTATCGGAGTGGGGTGAAGATTGTTGTTGCTTATGGTGGAGCTCCAATTTCTCAGCAGTTGCGTATTCTGGATAGAGGTTGTGATATCCTTGTTGCAACACCCGGGCGCTTGGTGGACATGATTGAGAGGTCACGAGTTTCGCTGAGTAATATCAAGTATTTGGCATTAGATGAGGCTGATAGGATGTTGGACATGGGTTTTGAGCCCCAGATAAGGAGGATTGTTGAACAAATGGACATGCCTCGCTCTGGTGCAAGACAAACGTTGCTTTTCAGTGCTACATTCCCAGATGAAATACAGAGGCTCGCTGCAGATTTTCTGTCAAACTACATTTTTCTATCTGTTGGAAGAGTTGGATCTAGCACAGGTCTCATTGCACAAAAAGTTGAACTCGTTGAGGATATGGACAAAAGAAACCATTTGATGAAGTTGCTTAGCCAGCAGGCAAATGGAGCTCATGGAAAGGTTGCTTTAACCTTGGTGTTTGTGGAGACAAAGAGAAGTGCAGATTCTTTAGAAAACTGGTTACGCATGAATCGTTTTCCAGCTATAGCTATACATGGTGATAAAGTGCAAATGGAGAGGGAGCAAGCTTTGAGATTGTTTAAGTGTGGCAAGACTCCAATATTGGTGGCGACAGATGTTGCTTCACGAGGGCTAGACATCCCTCATGTTTCCCATGTCATCAACTTTGATCGATCAAAAAGCATAGATGACTACGTTCACAGGATTGGACGAACAGGGCGTGCTGGAAATTCAGGTCTTGCAACTGCTTTCTTCAATGCCAAGAACCAGCCACTCACCAAGGGACTGATCGAGCTGATGCAGGAGTCAAACCAGGAGGTTCCAAGCTGGCTGAACGAATATGCTGAGGGCGAATCACAGACGTACAGCAGTGGTGGTGGTGGCCGGAACAGGAGGTCTGGTGGCAGTAAGTTTGGTGGCTATGACTATCGTAGGGACTCTCAATCTGCGCATGACAGTTATTACGGTCCAAGCAGCTATGGCTATGGAGATTATTCTGGGGGCATGTCTGCTGCTCCCTATAACGATGCTACTGCTGCTGTTCCAAACAGCTACGGAAACTATGAAACTATTGTTGCTGGTGGGTGGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04622 ko05161 ko05203 map04622 map05161 map05203
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

624

Amino Acids

67.86

Weight (kDa)

6.78

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 446
Acc16I TGCGCA 1 cut(s) 1728
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccBSI CCGCTC 1 cut(s) 414
AccII CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
AclI AACGTT 1 cut(s) 1016
AclWI GGATC 2 cut(s) 675, 1117
AcoI YGGCCR 2 cut(s) 199, 1660
AcsI RAATTY 2 cut(s) 323, 1507
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 6 cut(s) 75, 83, 116, 155, 500, 1643
AfiI CCNNNNNNNGG 5 cut(s) 218, 406, 659, 966, 1001
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 5 cut(s) 475, 631, 980, 1142, 1534
AjiI CACGTC 1 cut(s) 245
AjnI CCWGG 1 cut(s) 1590
Alw21I GWGCWC 3 cut(s) 574, 796, 1210
Alw26I GTCTC 3 cut(s) 705, 1127, 1238
AlwI GGATC 2 cut(s) 675, 1117
Ama87I CYCGRG 1 cut(s) 854
AoxI GGCC 4 cut(s) 147, 199, 564, 1660
ApeKI GCWGC 5 cut(s) 596, 1061, 1752, 1790, 1814
ApoI RAATTY 2 cut(s) 323, 1507
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
Asp718I GGTACC 1 cut(s) 81
AspLEI GCGC 3 cut(s) 133, 861, 1729
AspS9I GGNCC 6 cut(s) 15, 148, 182, 209, 582, 1745
AsuC2I CCSGG 5 cut(s) 186, 213, 401, 855, 856
AsuHPI GGTGA 4 cut(s) 19, 778, 1328, 1546
AvaI CYCGRG 1 cut(s) 854
AvaII GGWCC 5 cut(s) 15, 182, 209, 582, 1745
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 683
BaeI ACNNNNGTAYC 2 cut(s) 65, 98
BanI GGYRCC 1 cut(s) 81
BanII GRGCYC 4 cut(s) 268, 796, 960, 1210
BauI CACGAG 2 cut(s) 885, 1404
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 3 cut(s) 574, 796, 1210
BbvCI CCTCAGC 1 cut(s) 1623
BbvI GCAGC 5 cut(s) 583, 1048, 1764, 1777, 1801
BccI CCATC 2 cut(s) 353, 556
BceAI ACGGC 4 cut(s) 118, 139, 172, 609
BcgI CGANNNNNNTGC 2 cut(s) 1793, 1827
BciT130I CCWGG 1 cut(s) 1592
BclI TGATCA 1 cut(s) 328
BcnI CCSGG 5 cut(s) 186, 213, 401, 855, 856
BcoDI GTCTC 3 cut(s) 705, 1127, 1238
BfaI CTAG 2 cut(s) 1113, 1412
BfmI CTRYAG 2 cut(s) 1062, 1302
BfoI RGCGCY 1 cut(s) 862
BlpI GCTNAGC 1 cut(s) 1187
Bme1390I CCNGG 6 cut(s) 186, 213, 401, 855, 856, 1592
Bme18I GGWCC 5 cut(s) 15, 182, 209, 582, 1745
BmeT110I CYCGRG 1 cut(s) 854
BmgBI CACGTC 1 cut(s) 245
BmgT120I GGNCC 6 cut(s) 15, 148, 182, 209, 582, 1745
BmiI GGNNCC 5 cut(s) 44, 83, 184, 583, 1599
BmrFI CCNGG 6 cut(s) 186, 213, 401, 855, 856, 1592
BmrI ACTGGG 2 cut(s) 145, 588
BmsI GCATC 2 cut(s) 1566, 1795
BmuI ACTGGG 2 cut(s) 145, 588
BoxI GACNNNNGTC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 1623
Bpu1102I GCTNAGC 1 cut(s) 1187
BpuMI CCSGG 5 cut(s) 186, 213, 401, 855, 856
Bsa29I ATCGAT 2 cut(s) 456, 1450
BsaAI YACGTR 2 cut(s) 73, 502
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 6 cut(s) 11, 77, 543, 854, 1230, 1557
BsaXI ACNNNNNCTCC 2 cut(s) 1663, 1693
Bsc4I CCNNNNNNNGG 5 cut(s) 218, 406, 659, 966, 1001
Bse118I RCCGGY 3 cut(s) 197, 433, 652
Bse1I ACTGG 3 cut(s) 151, 583, 1280
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 1125
BseBI CCWGG 1 cut(s) 1592
BseCI ATCGAT 2 cut(s) 456, 1450
BseDI CCNNGG 6 cut(s) 11, 77, 543, 854, 1230, 1557
BseGI GGATG 3 cut(s) 396, 942, 1416
BseLI CCNNNNNNNGG 5 cut(s) 218, 406, 659, 966, 1001
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 6 cut(s) 309, 336, 587, 818, 887, 1614
BseNI ACTGG 3 cut(s) 151, 583, 1280
BseSI GKGCMC 1 cut(s) 683
BseX3I CGGCCG 1 cut(s) 199
BseXI GCAGC 5 cut(s) 583, 1048, 1764, 1777, 1801
BsgI GTGCAG 1 cut(s) 1278
Bsh1236I CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
Bsh1285I CGRYCG 2 cut(s) 202, 256
BshFI GGCC 4 cut(s) 149, 201, 566, 1662
BshNI GGYRCC 1 cut(s) 81
BshVI ATCGAT 2 cut(s) 456, 1450
BsiEI CGRYCG 2 cut(s) 202, 256
BsiHKAI GWGCWC 3 cut(s) 574, 796, 1210
BsiHKCI CYCGRG 1 cut(s) 854
BsiWI CGTACG 1 cut(s) 114
BslFI GGGAC 4 cut(s) 168, 299, 1575, 1727
BslI CCNNNNNNNGG 5 cut(s) 218, 406, 659, 966, 1001
BsmAI GTCTC 3 cut(s) 705, 1127, 1238
BsmFI GGGAC 4 cut(s) 168, 299, 1575, 1727
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 4 cut(s) 149, 201, 566, 1662
Bso31I GGTCTC 1 cut(s) 1127
BsoBI CYCGRG 1 cut(s) 854
Bsp1286I GDGCHC 6 cut(s) 268, 574, 683, 796, 960, 1210
Bsp143I GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
Bsp1720I GCTNAGC 1 cut(s) 1187
Bsp19I CCATGG 2 cut(s) 11, 543
Bsp68I TCGCGA 1 cut(s) 555
BspANI GGCC 4 cut(s) 149, 201, 566, 1662
BspCNI CTCAG 6 cut(s) 310, 337, 586, 817, 888, 1615
BspDI ATCGAT 2 cut(s) 456, 1450
BspFNI CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
BspHI TCATGA 1 cut(s) 730
BspLI GGNNCC 5 cut(s) 44, 83, 184, 583, 1599
BspMAI CTGCAG 1 cut(s) 1066
BspPI GGATC 2 cut(s) 675, 1117
BspT107I GGYRCC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 1127
BsrBI CCGCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 1125
BsrFI RCCGGY 3 cut(s) 197, 433, 652
BsrI ACTGG 3 cut(s) 151, 583, 1280
BssAI RCCGGY 3 cut(s) 197, 433, 652
BssECI CCNNGG 6 cut(s) 11, 77, 543, 854, 1230, 1557
BssMI GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 2 cut(s) 885, 1404
BssT1I CCWWGG 4 cut(s) 11, 543, 1230, 1557
Bst2BI CACGAG 2 cut(s) 885, 1404
Bst2UI CCWGG 1 cut(s) 1592
Bst4CI ACNGT 6 cut(s) 95, 119, 209, 638, 1736, 1745
Bst6I CTCTTC 1 cut(s) 1096
BstACI GRCGYC 1 cut(s) 384
BstBAI YACGTR 2 cut(s) 73, 502
BstDEI CTNAG 8 cut(s) 318, 345, 573, 741, 804, 896, 1187, 1623
BstDSI CCRYGG 2 cut(s) 11, 543
BstF5I GGATG 3 cut(s) 396, 942, 1416
BstFNI CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
BstH2I RGCGCY 1 cut(s) 862
BstHHI GCGC 3 cut(s) 133, 861, 1729
BstKTI GATC 7 cut(s) 331, 456, 670, 1112, 1450, 1454, 1569
BstMAI GTCTC 3 cut(s) 705, 1127, 1238
BstMBI GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
BstMCI CGRYCG 2 cut(s) 202, 256
BstMWI GCNNNNNNNGC 6 cut(s) 520, 678, 1192, 1205, 1758, 1814
BstNI CCWGG 1 cut(s) 1592
BstNSI RCATGY 2 cut(s) 994, 1786
BstPAI GACNNNNGTC 1 cut(s) 61
BstSCI CCNGG 6 cut(s) 184, 211, 399, 853, 854, 1590
BstSFI CTRYAG 2 cut(s) 1062, 1302
BstSLI GKGCMC 1 cut(s) 683
BstSNI TACGTA 1 cut(s) 73
BstUI CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
BstV1I GCAGC 5 cut(s) 583, 1048, 1764, 1777, 1801
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 1109
BstYI RGATCY 1 cut(s) 1109
BstZI CGGCCG 1 cut(s) 199
Bsu15I ATCGAT 2 cut(s) 456, 1450
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 149, 201, 566, 1662
BsuTUI ATCGAT 2 cut(s) 456, 1450
BtgI CCRYGG 2 cut(s) 11, 543
BtgZI GCGATG 1 cut(s) 372
BtrI CACGTC 1 cut(s) 245
BtsCI GGATG 3 cut(s) 396, 942, 1416
BtsI GCAGTG 1 cut(s) 1654
BtsIMutI CAGTG 5 cut(s) 100, 342, 625, 1033, 1654
BtuMI TCGCGA 1 cut(s) 555
CciI TCATGA 1 cut(s) 730
CfoI GCGC 3 cut(s) 133, 861, 1729
Cfr10I RCCGGY 3 cut(s) 197, 433, 652
Cfr13I GGNCC 6 cut(s) 15, 148, 182, 209, 582, 1745
Cfr9I CCCGGG 1 cut(s) 854
ClaI ATCGAT 2 cut(s) 456, 1450
CpoI CGGWCCG 1 cut(s) 209
CseI GACGC 1 cut(s) 392
Csp6I GTAC 6 cut(s) 74, 82, 115, 154, 499, 1642
CspCI CAANNNNNGTGG 2 cut(s) 1365, 1400
CspI CGGWCCG 1 cut(s) 209
CviQI GTAC 6 cut(s) 74, 82, 115, 154, 499, 1642
DdeI CTNAG 8 cut(s) 318, 345, 573, 741, 804, 896, 1187, 1623
DpnI GATC 7 cut(s) 330, 455, 669, 1111, 1449, 1453, 1568
DpnII GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
DrdI GACNNNNNNGTC 1 cut(s) 446
DseDI GACNNNNNNGTC 1 cut(s) 446
EaeI YGGCCR 2 cut(s) 199, 1660
EagI CGGCCG 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 1096
EarI CTCTTC 1 cut(s) 1096
EciI GGCGGA 2 cut(s) 240, 417
Ecl136II GAGCTC 2 cut(s) 794, 1208
EclXI CGGCCG 1 cut(s) 199
Eco105I TACGTA 1 cut(s) 73
Eco130I CCWWGG 4 cut(s) 11, 543, 1230, 1557
Eco24I GRGCYC 4 cut(s) 268, 796, 960, 1210
Eco31I GGTCTC 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 5 cut(s) 15, 182, 209, 582, 1745
Eco52I CGGCCG 1 cut(s) 199
Eco53kI GAGCTC 2 cut(s) 794, 1208
Eco81I CCTNAGG 1 cut(s) 318
Eco88I CYCGRG 1 cut(s) 854
EcoICRI GAGCTC 2 cut(s) 794, 1208
EcoRII CCWGG 1 cut(s) 1590
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 4 cut(s) 11, 543, 1230, 1557
EcoT38I GRGCYC 4 cut(s) 268, 796, 960, 1210
ErhI CCWWGG 4 cut(s) 11, 543, 1230, 1557
FaqI GGGAC 4 cut(s) 168, 299, 1575, 1727
FauI CCCGC 1 cut(s) 653
FbaI TGATCA 1 cut(s) 328
FokI GGATG 3 cut(s) 383, 949, 1403
FriOI GRGCYC 4 cut(s) 268, 796, 960, 1210
FspBI CTAG 2 cut(s) 1113, 1412
FspI TGCGCA 1 cut(s) 1728
GlaI GCGC 3 cut(s) 132, 860, 1728
HaeII RGCGCY 1 cut(s) 862
HaeIII GGCC 4 cut(s) 149, 201, 566, 1662
HgaI GACGC 1 cut(s) 392
HhaI GCGC 3 cut(s) 133, 861, 1729
Hin1I GRCGYC 1 cut(s) 384
Hin6I GCGC 3 cut(s) 131, 859, 1727
HinP1I GCGC 3 cut(s) 131, 859, 1727
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 1344
HphI GGTGA 4 cut(s) 19, 778, 1328, 1546
Hpy166II GTNNAC 4 cut(s) 36, 439, 868, 1477
Hpy188I TCNGA 8 cut(s) 19, 42, 89, 537, 576, 705, 726, 759
Hpy188III TCNNGA 7 cut(s) 451, 554, 628, 731, 821, 885, 1404
Hpy8I GTNNAC 4 cut(s) 36, 439, 868, 1477
Hpy99I CGWCG 3 cut(s) 104, 386, 709
HpyAV CCTTC 3 cut(s) 34, 183, 347
HpyCH4III ACNGT 6 cut(s) 95, 119, 209, 638, 1736, 1745
HpyCH4IV ACGT 7 cut(s) 72, 244, 443, 501, 1016, 1473, 1640
HpyCH4V TGCA 9 cut(s) 495, 848, 1007, 1064, 1130, 1259, 1327, 1520, 1579
HpyF10VI GCNNNNNNNGC 6 cut(s) 520, 678, 1192, 1205, 1758, 1814
HpyF3I CTNAG 8 cut(s) 318, 345, 573, 741, 804, 896, 1187, 1623
HpySE526I ACGT 7 cut(s) 72, 244, 443, 501, 1016, 1473, 1640
Hsp92I GRCGYC 1 cut(s) 384
HspAI GCGC 3 cut(s) 131, 859, 1727
KpnI GGTACC 1 cut(s) 85
KroI GCCGGC 2 cut(s) 197, 652
KroNI GCCGGC 2 cut(s) 199, 654
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
LmnI GCTCC 5 cut(s) 791, 799, 1205, 1339, 1798
Lsp1109I GCAGC 5 cut(s) 583, 1048, 1764, 1777, 1801
LweI GCATC 2 cut(s) 1566, 1795
MaeI CTAG 2 cut(s) 1113, 1412
MaeII ACGT 7 cut(s) 72, 244, 443, 501, 1016, 1473, 1640
MaeIII GTNAC 4 cut(s) 119, 340, 882, 1278
MalI GATC 7 cut(s) 330, 455, 669, 1111, 1449, 1453, 1568
MbiI CCGCTC 1 cut(s) 414
MboI GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
MboII GAAGA 4 cut(s) 404, 781, 1113, 1522
MflI RGATCY 1 cut(s) 1109
MhlI GDGCHC 6 cut(s) 268, 574, 683, 796, 960, 1210
MluCI AATT 4 cut(s) 323, 376, 798, 1507
MlyI GAGTC 6 cut(s) 41, 43, 152, 1366, 1592, 1709
MmeI TCCRAC 4 cut(s) 728, 921, 1078, 1087
MroNI GCCGGC 2 cut(s) 197, 652
MseI TTAA 2 cut(s) 1226, 1359
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 523
MspR9I CCNGG 6 cut(s) 186, 213, 401, 855, 856, 1592
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 1 cut(s) 1592
MvnI CGCG 6 cut(s) 131, 219, 240, 555, 660, 672
MwoI GCNNNNNNNGC 6 cut(s) 520, 678, 1192, 1205, 1758, 1814
NaeI GCCGGC 2 cut(s) 199, 654
NciI CCSGG 5 cut(s) 186, 213, 401, 855, 856
NcoI CCATGG 2 cut(s) 11, 543
NdeII GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
NgoMIV GCCGGC 2 cut(s) 197, 652
NlaIV GGNNCC 5 cut(s) 44, 83, 184, 583, 1599
NmuCI GTSAC 2 cut(s) 340, 882
NruI TCGCGA 1 cut(s) 555
NsbI TGCGCA 1 cut(s) 1728
NspI RCATGY 2 cut(s) 994, 1786
PagI TCATGA 1 cut(s) 730
PcsI WCGNNNNNNNCGW 4 cut(s) 85, 387, 1288, 1495
PctI GAATGC 1 cut(s) 473
PdiI GCCGGC 2 cut(s) 199, 654
PfeI GAWTC 6 cut(s) 20, 517, 727, 1262, 1288, 1631
Pfl23II CGTACG 1 cut(s) 114
PflFI GACNNNGTC 2 cut(s) 234, 580
PfoI TCCNGGA 1 cut(s) 184
PleI GAGTC 6 cut(s) 40, 43, 151, 1366, 1591, 1709
PpsI GAGTC 6 cut(s) 40, 43, 151, 1366, 1591, 1709
Ppu21I YACGTR 2 cut(s) 73, 502
PshAI GACNNNNGTC 1 cut(s) 61
Psp124BI GAGCTC 2 cut(s) 796, 1210
Psp1406I AACGTT 1 cut(s) 1016
Psp6I CCWGG 1 cut(s) 1590
PspGI CCWGG 1 cut(s) 1590
PspLI CGTACG 1 cut(s) 114
PspN4I GGNNCC 5 cut(s) 44, 83, 184, 583, 1599
PspPI GGNCC 6 cut(s) 15, 148, 182, 209, 582, 1745
PstI CTGCAG 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 1109
PsyI GACNNNGTC 2 cut(s) 234, 580
RruI TCGCGA 1 cut(s) 555
RsaI GTAC 6 cut(s) 75, 83, 116, 155, 500, 1643
RsaNI GTAC 6 cut(s) 74, 82, 115, 154, 499, 1642
RseI CAYNNNNRTG 1 cut(s) 401
Rsr2I CGGWCCG 1 cut(s) 209
RsrII CGGWCCG 1 cut(s) 209
SacI GAGCTC 2 cut(s) 796, 1210
SaqAI TTAA 2 cut(s) 1226, 1359
Sau3AI GATC 7 cut(s) 328, 453, 667, 1109, 1447, 1451, 1566
Sau96I GGNCC 6 cut(s) 15, 148, 182, 209, 582, 1745
SchI GAGTC 6 cut(s) 41, 43, 152, 1366, 1592, 1709
ScrFI CCNGG 6 cut(s) 186, 213, 401, 855, 856, 1592
SduI GDGCHC 6 cut(s) 268, 574, 683, 796, 960, 1210
SfaNI GCATC 2 cut(s) 1566, 1795
SfcI CTRYAG 2 cut(s) 1062, 1302
SgrAI CRCCGGYG 1 cut(s) 433
SinI GGWCC 5 cut(s) 15, 182, 209, 582, 1745
SmaI CCCGGG 1 cut(s) 856
SmiMI CAYNNNNRTG 1 cut(s) 401
SnaBI TACGTA 1 cut(s) 73
Sse9I AATT 4 cut(s) 323, 376, 798, 1507
SspI AATATT 1 cut(s) 1380
SspMI CTAG 2 cut(s) 1113, 1412
SstI GAGCTC 2 cut(s) 796, 1210
StyD4I CCNGG 6 cut(s) 184, 211, 399, 853, 854, 1590
StyI CCWWGG 4 cut(s) 11, 543, 1230, 1557
TaaI ACNGT 6 cut(s) 95, 119, 209, 638, 1736, 1745
TaiI ACGT 7 cut(s) 75, 247, 446, 504, 1019, 1476, 1643
TaqI TCGA 5 cut(s) 381, 450, 456, 1450, 1569
TasI AATT 4 cut(s) 323, 376, 798, 1507
TauI GCSGC 6 cut(s) 222, 225, 417, 433, 526, 675
TfiI GAWTC 6 cut(s) 20, 517, 727, 1262, 1288, 1631
Tru1I TTAA 2 cut(s) 1226, 1359
Tru9I TTAA 2 cut(s) 1226, 1359
TscAI CASTG 5 cut(s) 100, 349, 625, 1033, 1654
TseFI GTSAC 2 cut(s) 340, 882
TseI GCWGC 5 cut(s) 596, 1061, 1752, 1790, 1814
Tsp45I GTSAC 2 cut(s) 340, 882
TspDTI ATGAA 5 cut(s) 719, 1059, 1193, 1301, 1857
TspGWI ACGGA 1 cut(s) 1848
TspMI CCCGGG 1 cut(s) 854
TspRI CASTG 5 cut(s) 100, 349, 625, 1033, 1654
Tth111I GACNNNGTC 2 cut(s) 234, 580
VpaK11BI GGWCC 5 cut(s) 15, 182, 209, 582, 1745
XapI RAATTY 2 cut(s) 323, 1507
XceI RCATGY 2 cut(s) 994, 1786
XcmI CCANNNNNNNNNTGG 1 cut(s) 1199
XmaI CCCGGG 1 cut(s) 854
XspI CTAG 2 cut(s) 1113, 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.