MD03G1094200.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000494
Physical Location & Seq
Reverse (-)
7875680 .. 7878153
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1094200.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGTTTTTGGTCGGCGAAGATGGATCTGGATCCACGTTGCATTCCACAAATGAATCCGGAAAGACTCCCTTGTGCATGGCTGCTGAAACTGCCAATCTTGAACTTGTTAAAGCAATGGTAGACAAGACGCCAAATAGCACAACAGACGCTAATTCTGGTAATTTTTGGCAAGGATCGAACGGCAACTCAATTCTTCGTAGTGCGATCATATGGAGGAAACAAGCAAAAGGTGGCCTCAAGAAATATGATGCAGTGTTCGTGTTGGTCATGCTCATGTTATGGAATTTCAACATTCCACTTTTTTTTGTGCTGGCGGGGGTTGTATGCTTGATTGAAGTTTCTCAATGGTGGCTGCCCAGAACCAACAGAAAGAAGTATACAAATATACCTCTGTCATTGCTATTTTCTATAGCAGTGTTCGCGTTGGTCATGTCCATATTAATCAATCTCATCAATCCACTCTTTGTGATGGCGGGGGTTGTATGCTGGATATTTTTTAAGAGTATAGTTTCTCAATGGTGGCTGCCCAGAGCCAACAGAAAGAAGTATACAAATCTCCTAGAGACGATATTGAGCAAAATGGATTCAGCTGATCTCATCAATTCAATGGACCAAGGGTGGATGTCTCCTCTTACAGTTGCGGCTTATATGGATAACTTTGTGGCGGTACGCATATTGTTGCACAAATTTACTGGATTGGCATACAAAGTTTCTCGATTATTGCCCCGACTTGAGGGATCAAGTGACCATGAAGGCCGGAATATTCTTCATCTTGCGGCCGCGCATGGAAGAGTTGATGTTGTCCGATATATTATTGGAAAGCGCGATCTTCAAGTGCTTAGCAAGCAAAGAGATCAAAGTGGAAATACCCCTCTACATATAGCCGTCCAAAACTGGCATCCTAAGATCGTCAAAATTCTTTCCCTCTTCCATGCGTATGAGAGAGCCAATTTAAATAGTTTGAACAATGCAGGTGAGACGGCATTGGACCTTGCAGAGAAGACCGGGAAACTAACACTTATGGCCTTGAACTTGGCTAATGCTCCACGATCTGAAGAGCGAAAAGTCTCCAAAGAGACACAGGATTCTGTTCCGAAGTCCTTGCACAATTCTGATAACCTAATGTTTTCGAAAGAGAGTGTCAATACTCTCCTATTGGTGTCAACGCTGGTGGTTGGTATAACGTTTGCTCAAGGCTTCACAGTACCAGGTGGATACAGTGACACTGGAAATAATGTGGGCACCCCAAATTTTCTTCCGCATAGCCTATTCAGAATTTTCTTGATATGCAATACCGTGGCTATGTGCGGCTCCATTACTGCTTCAATTGCCCTTATGTGGGCGCAAACATATGACCCTAGTATAATCTATGCTGCCATGAGATTTACCTTACCGGTGCTAGGCGTAACGCTTACCATGATGTCCTCGTCATTCCTGGCTGGTGTTGCACTGACAGTGTATCACGTTTTATGGCTTCGCATCTTTGTTTATGCTATGGGATTGTCCTTCACTGTCTTCATAGTAGGGCTCCTTGCTCCTCTCATAGATCTTCCAGTTTCCTACGAAACTATCCCAACACGCGCCATCTTATTCTTCTGCTTTGATCTATTAATGTTTGTCGGCGGATATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0002831 GO:0002833 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009966 GO:0009987 GO:0010033 GO:0010646 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016567 GO:0019538 GO:0023051 GO:0030176 GO:0031224 GO:0031226 GO:0031227 GO:0031347 GO:0031349 GO:0031984 GO:0032101 GO:0032103 GO:0032446 GO:0032870 GO:0035690 GO:0036211 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045087 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0098827 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901700 GO:1901701 GO:1902288 GO:1902290 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

545

Amino Acids

60.34

Weight (kDa)

9.27

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 182 - 274 6.8e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 256 - 307 2.1e-07 Ankyrin repeats (many copies)
PGG PF13962 378 - 484 3.1e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03670 AT4G03440 AT4G03440 AT4G03440 AT4G03450 AT4G03450 AT4G03460 AT4G03460 AT4G03470 AT4G03480 AT4G03480 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03500 AT4G03500 AT4G03500 AT4G03505 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G14390 AT4G14390 AT4G14390 AT4G14390 AT4G14400 AT4G14400 AT4G14400
fragaria_vesca FvH4_3g05910 FvH4_3g23320
malus_domestica MD03G1094000.v1.1 MD03G1094200.v1.1 MD03G1094600.v1.1 MD12G1218700.v1.1
prunus_persica Prupe.3G135100_v2.0.a1 Prupe.3G135200_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G142400_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G154000_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154600_v2.0.a1 Prupe.3G154700_v2.0.a1 Prupe.3G155200_v2.0.a1 Prupe.3G155300_v2.0.a1 Prupe.8G110300_v2.0.a1
pyrus_communis pycom03g07280 pycom03g07580 pycom03g07660 pycom03g07700 pycom03g07710
rosa_chinensis RchiOBHm_Chr3g0461161 RchiOBHm_Chr5g0041111
rosa_laevigata RLG00000023472 RLG00000023475 RLG00000034025
rosa_multiflora Rmu_co8126040.1_g000001 Rmu_co8268563.1_g000001 Rmu_sc0000964.1_g000014 Rmu_sc0028923.1_g000001 Rmu_sc0042194.1_g000001
rosa_roxburghii Rroxscaffold_1G00039610 Rroxscaffold_6G00401360
rosa_rugosa Rorug03G0048600 Rorug03G0048700 Rorug05G0190000
rosa_samantha Rh3AG105900 Rh3BG110200 Rh3BG268400 Rh3CG111800 Rh3DG111900 Rh5AG275800 Rh5BG280500 Rh5CG312600 Rh5DG288800
rosa_wichuraiana Rw3G009080 Rw5G025920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 962
Acc36I ACCTGC 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 1241
AccI GTMKAC 3 cut(s) 120, 377, 548
AccII CGCG 4 cut(s) 422, 782, 825, 1581
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 9 cut(s) 314, 473, 641, 665, 776, 780, 1259, 1308, 1623
AclI AACGTT 1 cut(s) 1184
AclWI GGATC 5 cut(s) 24, 31, 37, 181, 745
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 5 cut(s) 283, 686, 915, 1249, 1275
AcuI CTGAAG 1 cut(s) 1074
AcyI GRCGYC 1 cut(s) 128
AfaI GTAC 2 cut(s) 669, 1206
AfiI CCNNNNNNNGG 4 cut(s) 733, 1338, 1339, 1400
AgeI ACCGGT 1 cut(s) 1393
AgsI TTSAA 8 cut(s) 101, 289, 335, 606, 833, 964, 1030, 1326
AjnI CCWGG 2 cut(s) 1207, 1434
AluBI AGCT 1 cut(s) 590
AluI AGCT 1 cut(s) 590
Alw26I GTCTC 5 cut(s) 557, 630, 971, 1070, 1072
AlwI GGATC 5 cut(s) 24, 31, 37, 181, 745
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 4 cut(s) 232, 754, 777, 1023
ApeKI GCWGC 4 cut(s) 80, 352, 523, 1373
ApoI RAATTY 5 cut(s) 283, 686, 915, 1249, 1275
AseI ATTAAT 2 cut(s) 440, 1610
AsiGI ACCGGT 1 cut(s) 1393
AspLEI GCGC 4 cut(s) 784, 825, 1345, 1583
AspS9I GGNCC 2 cut(s) 610, 988
AsuC2I CCSGG 1 cut(s) 1006
AsuHPI GGTGA 1 cut(s) 986
AsuII TTCGAA 1 cut(s) 1130
AvaII GGWCC 2 cut(s) 610, 988
BaeGI GKGCMC 1 cut(s) 1244
BamHI GGATCC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 1241
BanII GRGCYC 1 cut(s) 1530
BbsI GAAGAC 2 cut(s) 1007, 1507
BbvI GCAGC 4 cut(s) 67, 339, 510, 1360
BccI CCATC 3 cut(s) 14, 463, 1592
BceAI ACGGC 3 cut(s) 196, 869, 996
BcgI CGANNNNNNTGC 2 cut(s) 1084, 1118
BciT130I CCWGG 2 cut(s) 1209, 1436
BciVI GTATCC 1 cut(s) 1208
BcnI CCSGG 1 cut(s) 1006
BcoDI GTCTC 5 cut(s) 557, 630, 971, 1070, 1072
BfaI CTAG 3 cut(s) 560, 1359, 1400
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 962
BfuI GTATCC 1 cut(s) 1208
BglII AGATCT 1 cut(s) 1546
BisI GCNGC 8 cut(s) 81, 353, 524, 642, 777, 780, 1309, 1374
BlpI GCTNAGC 1 cut(s) 839
BlsI GCNGC 8 cut(s) 82, 354, 525, 643, 778, 781, 1310, 1375
Bme1390I CCNGG 3 cut(s) 1006, 1209, 1436
Bme18I GGWCC 2 cut(s) 610, 988
BmgT120I GGNCC 2 cut(s) 610, 988
BmiI GGNNCC 4 cut(s) 31, 1243, 1312, 1529
BmrFI CCNGG 3 cut(s) 1006, 1209, 1436
BmsI GCATC 3 cut(s) 238, 907, 1488
BpiI GAAGAC 2 cut(s) 1007, 1507
Bpu1102I GCTNAGC 1 cut(s) 839
Bpu14I TTCGAA 1 cut(s) 1130
BpuEI CTTGAG 3 cut(s) 221, 752, 1176
BpuMI CCSGG 1 cut(s) 1006
BsaBI GATNNNNATC 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 128
BsaJI CCNNGG 2 cut(s) 613, 1296
BsaWI WCCGGW 2 cut(s) 56, 1393
Bsc4I CCNNNNNNNGG 4 cut(s) 733, 1338, 1339, 1400
Bse118I RCCGGY 1 cut(s) 1393
Bse1I ACTGG 4 cut(s) 697, 899, 1231, 1553
Bse3DI GCAATG 2 cut(s) 120, 395
Bse8I GATNNNNATC 1 cut(s) 28
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 2 cut(s) 1209, 1436
BseDI CCNNGG 2 cut(s) 613, 1296
BseGI GGATG 2 cut(s) 627, 898
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 4 cut(s) 733, 1338, 1339, 1400
BseMI GCAATG 2 cut(s) 120, 395
BseNI ACTGG 4 cut(s) 697, 899, 1231, 1553
BseRI GAGGAG 2 cut(s) 618, 1527
BseSI GKGCMC 1 cut(s) 1244
BseX3I CGGCCG 1 cut(s) 777
BseXI GCAGC 4 cut(s) 67, 339, 510, 1360
Bsh1236I CGCG 4 cut(s) 422, 782, 825, 1581
Bsh1285I CGRYCG 1 cut(s) 780
BshFI GGCC 4 cut(s) 234, 756, 779, 1025
BshNI GGYRCC 1 cut(s) 1241
BshTI ACCGGT 1 cut(s) 1393
BsiEI CGRYCG 1 cut(s) 780
BsiSI CCGG 4 cut(s) 57, 757, 1005, 1394
BslI CCNNNNNNNGG 4 cut(s) 733, 1338, 1339, 1400
BsmAI GTCTC 5 cut(s) 557, 630, 971, 1070, 1072
BsmBI CGTCTC 2 cut(s) 557, 971
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 4 cut(s) 234, 756, 779, 1025
Bsp119I TTCGAA 1 cut(s) 1130
Bsp1286I GDGCHC 2 cut(s) 1244, 1530
Bsp13I TCCGGA 1 cut(s) 56
Bsp1720I GCTNAGC 1 cut(s) 839
BspACI CCGC 9 cut(s) 314, 473, 641, 665, 776, 780, 1259, 1308, 1623
BspANI GGCC 4 cut(s) 234, 756, 779, 1025
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 4 cut(s) 422, 782, 825, 1581
BspLI GGNNCC 4 cut(s) 31, 1243, 1312, 1529
BspMI ACCTGC 1 cut(s) 962
BspPI GGATC 5 cut(s) 24, 31, 37, 181, 745
BspQI GCTCTTC 1 cut(s) 1050
BspT104I TTCGAA 1 cut(s) 1130
BspT107I GGYRCC 1 cut(s) 1241
BsrDI GCAATG 2 cut(s) 120, 395
BsrFI RCCGGY 1 cut(s) 1393
BsrI ACTGG 4 cut(s) 697, 899, 1231, 1553
BssAI RCCGGY 1 cut(s) 1393
BssECI CCNNGG 2 cut(s) 613, 1296
BssNAI GTATAC 2 cut(s) 378, 549
BssNI GRCGYC 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 613
Bst1107I GTATAC 2 cut(s) 378, 549
Bst2UI CCWGG 2 cut(s) 1209, 1436
Bst4CI ACNGT 6 cut(s) 637, 1204, 1220, 1297, 1456, 1513
Bst6I CTCTTC 3 cut(s) 784, 932, 1050
BstACI GRCGYC 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 1130
BstC8I GCNNGC 2 cut(s) 312, 845
BstDEI CTNAG 2 cut(s) 839, 903
BstDSI CCRYGG 1 cut(s) 1296
BstF5I GGATG 2 cut(s) 627, 898
BstFNI CGCG 4 cut(s) 422, 782, 825, 1581
BstHHI GCGC 4 cut(s) 784, 825, 1345, 1583
BstMAI GTCTC 5 cut(s) 557, 630, 971, 1070, 1072
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 5 cut(s) 89, 419, 844, 1328, 1445
BstNI CCWGG 2 cut(s) 1209, 1436
BstSCI CCNGG 3 cut(s) 1004, 1207, 1434
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 1244
BstUI CGCG 4 cut(s) 422, 782, 825, 1581
BstV1I GCAGC 4 cut(s) 67, 339, 510, 1360
BstV2I GAAGAC 2 cut(s) 1007, 1507
BstX2I RGATCY 3 cut(s) 23, 29, 1546
BstYI RGATCY 3 cut(s) 23, 29, 1546
BstZ17I GTATAC 2 cut(s) 378, 549
BstZI CGGCCG 1 cut(s) 777
BsuI GTATCC 1 cut(s) 1208
BsuRI GGCC 4 cut(s) 234, 756, 779, 1025
BtgI CCRYGG 1 cut(s) 1296
BtsCI GGATG 2 cut(s) 627, 898
BtsI GCAGTG 2 cut(s) 258, 420
BtsIMutI CAGTG 7 cut(s) 258, 420, 1224, 1225, 1448, 1461, 1509
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 2 cut(s) 312, 845
CciNI GCGGCCGC 1 cut(s) 777
CfoI GCGC 4 cut(s) 784, 825, 1345, 1583
Cfr10I RCCGGY 1 cut(s) 1393
Cfr13I GGNCC 2 cut(s) 610, 988
CseI GACGC 2 cut(s) 136, 155
CsiI ACCWGGT 1 cut(s) 1207
Csp6I GTAC 2 cut(s) 668, 1205
CspAI ACCGGT 1 cut(s) 1393
CspCI CAANNNNNGTGG 2 cut(s) 1152, 1187
CviAII CATG 9 cut(s) 76, 268, 274, 430, 749, 785, 932, 1378, 1417
CviQI GTAC 2 cut(s) 668, 1205
DdeI CTNAG 2 cut(s) 839, 903
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 1 cut(s) 777
EagI CGGCCG 1 cut(s) 777
Eam1104I CTCTTC 3 cut(s) 784, 932, 1050
EarI CTCTTC 3 cut(s) 784, 932, 1050
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 613
Eco24I GRGCYC 1 cut(s) 1530
Eco47I GGWCC 2 cut(s) 610, 988
Eco52I CGGCCG 1 cut(s) 777
Eco57I CTGAAG 1 cut(s) 1074
EcoRII CCWGG 2 cut(s) 1207, 1434
EcoT14I CCWWGG 1 cut(s) 613
EcoT38I GRGCYC 1 cut(s) 1530
ErhI CCWWGG 1 cut(s) 613
Esp3I CGTCTC 2 cut(s) 557, 971
FaeI CATG 9 cut(s) 79, 271, 277, 433, 752, 788, 935, 1381, 1420
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FatI CATG 9 cut(s) 75, 267, 273, 429, 748, 784, 931, 1377, 1416
FauI CCCGC 2 cut(s) 307, 466
FauNDI CATATG 2 cut(s) 209, 1351
FblI GTMKAC 3 cut(s) 120, 377, 548
Fnu4HI GCNGC 8 cut(s) 81, 353, 524, 642, 777, 780, 1309, 1374
FokI GGATG 2 cut(s) 634, 885
FriOI GRGCYC 1 cut(s) 1530
Fsp4HI GCNGC 8 cut(s) 81, 353, 524, 642, 777, 780, 1309, 1374
FspBI CTAG 3 cut(s) 560, 1359, 1400
GlaI GCGC 4 cut(s) 783, 824, 1344, 1582
GluI GCNGC 8 cut(s) 81, 353, 524, 642, 777, 780, 1309, 1374
HaeIII GGCC 4 cut(s) 234, 756, 779, 1025
HapII CCGG 4 cut(s) 57, 757, 1005, 1394
HgaI GACGC 2 cut(s) 136, 155
HhaI GCGC 4 cut(s) 784, 825, 1345, 1583
Hin1I GRCGYC 1 cut(s) 128
Hin1II CATG 9 cut(s) 79, 271, 277, 433, 752, 788, 935, 1381, 1420
Hin6I GCGC 4 cut(s) 782, 823, 1343, 1581
HinP1I GCGC 4 cut(s) 782, 823, 1343, 1581
HincII GTYRAC 1 cut(s) 1164
HindII GTYRAC 1 cut(s) 1164
HinfI GANTC 4 cut(s) 53, 64, 584, 1085
HpaII CCGG 4 cut(s) 57, 757, 1005, 1394
HphI GGTGA 1 cut(s) 986
Hpy166II GTNNAC 4 cut(s) 121, 378, 549, 1164
Hpy188I TCNGA 5 cut(s) 806, 1054, 1095, 1114, 1274
Hpy188III TCNNGA 6 cut(s) 27, 57, 98, 238, 714, 1282
Hpy8I GTNNAC 4 cut(s) 121, 378, 549, 1164
HpyAV CCTTC 2 cut(s) 746, 1516
HpyCH4III ACNGT 6 cut(s) 637, 1204, 1220, 1297, 1456, 1513
HpyCH4IV ACGT 3 cut(s) 35, 1184, 1464
HpyCH4V TGCA 9 cut(s) 40, 75, 251, 682, 971, 995, 1105, 1290, 1448
HpyF10VI GCNNNNNNNGC 5 cut(s) 89, 419, 844, 1328, 1445
HpyF3I CTNAG 2 cut(s) 839, 903
HpySE526I ACGT 3 cut(s) 35, 1184, 1464
Hsp92I GRCGYC 1 cut(s) 128
Hsp92II CATG 9 cut(s) 79, 271, 277, 433, 752, 788, 935, 1381, 1420
HspAI GCGC 4 cut(s) 782, 823, 1343, 1581
Kpn2I TCCGGA 1 cut(s) 56
LguI GCTCTTC 1 cut(s) 1050
LmnI GCTCC 4 cut(s) 1048, 1316, 1533, 1540
Lsp1109I GCAGC 4 cut(s) 67, 339, 510, 1360
LweI GCATC 3 cut(s) 238, 907, 1488
MabI ACCWGGT 1 cut(s) 1207
MaeI CTAG 3 cut(s) 560, 1359, 1400
MaeII ACGT 3 cut(s) 35, 1184, 1464
MaeIII GTNAC 3 cut(s) 743, 1220, 1405
MfeI CAATTG 1 cut(s) 1326
MflI RGATCY 3 cut(s) 23, 29, 1546
MhlI GDGCHC 2 cut(s) 1244, 1530
MlyI GAGTC 1 cut(s) 58
MnlI CCTC 9 cut(s) 207, 245, 399, 639, 727, 882, 935, 1435, 1548
MroI TCCGGA 1 cut(s) 56
MseI TTAA 6 cut(s) 108, 440, 498, 953, 1610, 1633
MslI CAYNNNNRTG 2 cut(s) 272, 936
MspA1I CMGCKG 1 cut(s) 590
MspI CCGG 4 cut(s) 57, 757, 1005, 1394
MspR9I CCNGG 3 cut(s) 1006, 1209, 1436
MunI CAATTG 1 cut(s) 1326
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 2 cut(s) 1209, 1436
MvnI CGCG 4 cut(s) 422, 782, 825, 1581
MwoI GCNNNNNNNGC 5 cut(s) 89, 419, 844, 1328, 1445
NciI CCSGG 1 cut(s) 1006
NdeI CATATG 2 cut(s) 209, 1351
NlaIII CATG 9 cut(s) 79, 271, 277, 433, 752, 788, 935, 1381, 1420
NlaIV GGNNCC 4 cut(s) 31, 1243, 1312, 1529
NmuCI GTSAC 2 cut(s) 743, 1220
NotI GCGGCCGC 1 cut(s) 777
NspV TTCGAA 1 cut(s) 1130
PaqCI CACCTGC 1 cut(s) 962
PciSI GCTCTTC 1 cut(s) 1050
PctI GAATGC 1 cut(s) 40
PfeI GAWTC 3 cut(s) 53, 584, 1085
PinAI ACCGGT 1 cut(s) 1393
PkrI GCNGC 8 cut(s) 82, 354, 525, 643, 778, 781, 1310, 1375
PleI GAGTC 1 cut(s) 58
PpsI GAGTC 1 cut(s) 58
PshBI ATTAAT 2 cut(s) 440, 1610
Psp1406I AACGTT 1 cut(s) 1184
Psp6I CCWGG 2 cut(s) 1207, 1434
PspGI CCWGG 2 cut(s) 1207, 1434
PspN4I GGNNCC 4 cut(s) 31, 1243, 1312, 1529
PspPI GGNCC 2 cut(s) 610, 988
PsuI RGATCY 3 cut(s) 23, 29, 1546
PvuII CAGCTG 1 cut(s) 590
RsaI GTAC 2 cut(s) 669, 1206
RsaNI GTAC 2 cut(s) 668, 1205
RseI CAYNNNNRTG 2 cut(s) 272, 936
SapI GCTCTTC 1 cut(s) 1050
SaqAI TTAA 6 cut(s) 108, 440, 498, 953, 1610, 1633
SatI GCNGC 8 cut(s) 81, 353, 524, 642, 777, 780, 1309, 1374
Sau96I GGNCC 2 cut(s) 610, 988
SchI GAGTC 1 cut(s) 58
ScrFI CCNGG 3 cut(s) 1006, 1209, 1436
SduI GDGCHC 2 cut(s) 1244, 1530
SexAI ACCWGGT 1 cut(s) 1207
SfaNI GCATC 3 cut(s) 238, 907, 1488
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 1130
SinI GGWCC 2 cut(s) 610, 988
SmiI ATTTAAAT 1 cut(s) 954
SmiMI CAYNNNNRTG 2 cut(s) 272, 936
SmlI CTYRAG 3 cut(s) 236, 731, 1191
SmoI CTYRAG 3 cut(s) 236, 731, 1191
SsiI CCGC 9 cut(s) 314, 473, 641, 665, 776, 780, 1259, 1308, 1623
SspI AATATT 1 cut(s) 763
SspMI CTAG 3 cut(s) 560, 1359, 1400
StyD4I CCNGG 3 cut(s) 1004, 1207, 1434
StyI CCWWGG 1 cut(s) 613
SwaI ATTTAAAT 1 cut(s) 954
TaaI ACNGT 6 cut(s) 637, 1204, 1220, 1297, 1456, 1513
TaiI ACGT 3 cut(s) 38, 1187, 1467
TaqI TCGA 3 cut(s) 176, 715, 1130
TauI GCSGC 4 cut(s) 644, 779, 782, 1311
TfiI GAWTC 3 cut(s) 53, 584, 1085
Tru1I TTAA 6 cut(s) 108, 440, 498, 953, 1610, 1633
Tru9I TTAA 6 cut(s) 108, 440, 498, 953, 1610, 1633
TscAI CASTG 7 cut(s) 258, 420, 1225, 1231, 1455, 1461, 1516
TseFI GTSAC 2 cut(s) 743, 1220
TseI GCWGC 4 cut(s) 80, 352, 523, 1373
Tsp45I GTSAC 2 cut(s) 743, 1220
TspDTI ATGAA 4 cut(s) 66, 758, 765, 1507
TspRI CASTG 7 cut(s) 258, 420, 1225, 1231, 1455, 1461, 1516
VpaK11BI GGWCC 2 cut(s) 610, 988
VspI ATTAAT 2 cut(s) 440, 1610
XapI RAATTY 5 cut(s) 283, 686, 915, 1249, 1275
XmiI GTMKAC 3 cut(s) 120, 377, 548
XspI CTAG 3 cut(s) 560, 1359, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.