RLG00000023475

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000494
Physical Location & Seq
Forward (+)
24375883 .. 24380636
4754 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023475

Sequence Viewer

Length: 2262 bp
ATGGCCGAGGCCGCCGAGCTAGTGGTAGGTGCTTTTTTAGGGCACTTGGTGGCGTCCGTGCGTTCGCTGATCAAGAACTTATATGATACCTTGGTATTCCGTGTTGACCTTGAATATTTATTAGTATGGTTGCTGGATAGGCTTGCTTTGCTGGTGATTCAACCATCACAGACACCACTCCTGCGAAACTTGCACGAGTATGAGCAAATAACTGATCCTTTCCAAAAGTTGTACAGGGCTGCAAGTTTGGGCGATAAAGATGAATTTTTGGCAACCCTGCCCGCAACAAATGATGAAAATTCTTTCATCCAGCTCATCTGTCAACTGAGTCCCCTCAAGAACACATTTCTTCACATAGCAGTGAGCTATGGCCATGAAAATCTGGCAACTGAGATAGTCAAACTTCACCGTCCGCTTTTGCTTAAGAAAAATTTCCAAGGTGACACTGCACTTCATATTGCGGCGAAAGGTGGGAGACTGCATATGGTAGGAATTCTTCTCGGTTTAATGGCGAGAACAACTCAAAGTTCAGTTACAAGTGATGGCGAAACAATAGATATTGAAAATCATATGTCGTTGTTGGAGATGAAAAATGACGAGGGAAACACCGCCTTACATGAGGCATTGATTAATGGTCACGAAGGTGTGGCATGGCAGCTGATCAAGACCGATCCTTCCCTTTCATTTACCTCTAATAATGAGCAAAAATCACCTCTGTATCTCGCAACTGAGGCCAGGTTTCTTTCAATTGTTAAGCTGATCAACAGAACTGCAGATCAGAATAACACAAGTAGCATAGGCAAATCACCATTGCATGTTGCAATTCTTCACCGCAATAAAGAAATGATGGGGGAAATATCAAGTATGGGATTTACCATCAACCTAAGAGATGAGGAAGGGAGAAATCCACTTCATTATGCAGCGTCAATAGGTTATTTAGAAGGAGTTTGCTTTTTCTTACGCAAATGTTCATCAGAGTCTCATTATTGTAATCAACCTGATAATAGTGGCACACTTCCTATCTATTCTGCATCAAGAAAAGGCCATGTTGACATTGTTAGAGCGCTCCTTCAACATTTTCCTGCTTTAAAGGAGGAACCCATGAGTTCAGAAGGACAAAATTTGCTTCATGTTGCAGCAAAATTTGGAAGACATAATCTTGTTAGATATTTTCTTCAAAACAGGGATGAATTTCAGATGTTGATTAATCACAAAGATAACCATGGAAATACTCCTTTACATTTGGCGACAATTCACAAACATGCCAGAGTGGTGAAATATTTCACATGGGATAGAAGGACCAACTTAAACCTTTCCAACAGGAAAGACATGACAGCTCTTGATATTGCAGAGAGCACAAAGGAAGTAATTGGTTCATTTCGTGGGAGGCTAACTTGGGTAGCACTAAAATCTGCCGGTGCAAAGAGAGCTAAACATTTGCATGTTCTCCTACGAACCACACAGAGTCCTGCAGAAGATGTTGTAAGAAACACAGACGGACAAGTTGCATCCGAAAATGAAGATGTTGCTACTGAAATCATTAAAGCTTGTAGACCGGATAGAGAAAATTCACAGTTAGATGACAAGAAAAGTTATACAGACAGGGTCAACACACTACTGGTGATGACAACTCTTGTTGCTACAGTGACATTTGCAGCTGGTTTCACAATGCCCGGTGGTTATAACAACTCCAGCCCTCATGAAGGCAGAGCAACCTTGCTAACAAGATCTATGTTCCAAGTCTTTGTGATCTCCAACACCATAGCTATGTATAGCTCCGTCTTTGTGGCAGTTACACTCATTTGGGCACAATCTGGTGATCTGATTATGGTATTCACAGGTCTTCGTTTGGTAATGCCAGTGTTGGGGATTGCACTAGCCATGCTGTCGTTAGCATTTATGGCTGGCGTTTATGTGGTACTAAGCAATCTTCCTTGGCTTGCGTCCTTGGTGTTGGCCATCGGAATAGTTTTTCTCTACACAGTCTTGGCTTGTTATGTTATTTATAATTCAACAGTTCCAGAGAAAACCCTCTTCGGATCAGATTTGGTGACGATCCTGGGTCGCCTGCCGGATTGGCTTGTAGCGTCAATGACACAGCCTCTCCCTCTTCCCTCACCCGGAGGGCTTGTTTGTCAAAGCCTCGCTGGTGCTGCTGTCATCAAAGCCTGTGAAGGCTGTGATCTAGATTGGGTGGTCGACCTAGGGTTGTTTGGTTTCAGATTGGTGGTCCTTGTGTTAGGAGGATCGGAGGGGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

754

Amino Acids

83.22

Weight (kDa)

6.6

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 106 - 168 4.3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 196 - 262 1.1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 258 - 321 8.1e-07 Ankyrin repeats (3 copies)
Ank_4 PF13637 271 - 320 8.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 293 - 359 4.2e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 330 - 394 2e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 370 - 435 6.5e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 376 - 428 1.6e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 398 - 450 1.2e-06 Ankyrin repeats (many copies)
PGG PF13962 530 - 640 4.4e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03670 AT4G03440 AT4G03440 AT4G03440 AT4G03450 AT4G03450 AT4G03460 AT4G03460 AT4G03470 AT4G03480 AT4G03480 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03500 AT4G03500 AT4G03500 AT4G03505 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G14390 AT4G14390 AT4G14390 AT4G14390 AT4G14400 AT4G14400 AT4G14400
fragaria_vesca FvH4_3g05910 FvH4_3g23320
malus_domestica MD03G1094000.v1.1 MD03G1094200.v1.1 MD03G1094600.v1.1 MD12G1218700.v1.1
prunus_persica Prupe.3G135100_v2.0.a1 Prupe.3G135200_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G142400_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G154000_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154600_v2.0.a1 Prupe.3G154700_v2.0.a1 Prupe.3G155200_v2.0.a1 Prupe.3G155300_v2.0.a1 Prupe.8G110300_v2.0.a1
pyrus_communis pycom03g07280 pycom03g07580 pycom03g07660 pycom03g07700 pycom03g07710
rosa_chinensis RchiOBHm_Chr3g0461161 RchiOBHm_Chr5g0041111
rosa_laevigata RLG00000023472 RLG00000023475 RLG00000034025
rosa_multiflora Rmu_co8126040.1_g000001 Rmu_co8268563.1_g000001 Rmu_sc0000964.1_g000014 Rmu_sc0028923.1_g000001 Rmu_sc0042194.1_g000001
rosa_roxburghii Rroxscaffold_1G00039610 Rroxscaffold_6G00401360
rosa_rugosa Rorug03G0048600 Rorug03G0048700 Rorug05G0190000
rosa_samantha Rh3AG105900 Rh3BG110200 Rh3BG268400 Rh3CG111800 Rh3DG111900 Rh5AG275800 Rh5BG280500 Rh5CG312600 Rh5DG288800
rosa_wichuraiana Rw3G009080 Rw5G025920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1683, 2007
AccI GTMKAC 2 cut(s) 1552, 2199
AciI CCGC 6 cut(s) 12, 282, 413, 461, 609, 832
AclWI GGATC 5 cut(s) 209, 665, 2047, 2050, 2254
AcoI YGGCCR 3 cut(s) 3, 370, 1956
AcsI RAATTY 8 cut(s) 263, 298, 430, 492, 1120, 1142, 1190, 1567
AcyI GRCGYC 1 cut(s) 53
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 233, 1920
AfeI AGCGCT 1 cut(s) 1065
AfiI CCNNNNNNNGG 3 cut(s) 1703, 1865, 2120
AflII CTTAAG 1 cut(s) 422
AgsI TTSAA 7 cut(s) 113, 161, 563, 747, 1073, 1178, 2013
AjnI CCWGG 2 cut(s) 734, 2058
AleI CACNNNNGTG 1 cut(s) 642
Alw21I GWGCWC 1 cut(s) 1358
Alw26I GTCTC 2 cut(s) 469, 984
AlwI GGATC 5 cut(s) 209, 665, 2047, 2050, 2254
Aor51HI AGCGCT 1 cut(s) 1065
AoxI GGCC 6 cut(s) 3, 9, 370, 732, 1042, 1956
ApeKI GCWGC 6 cut(s) 239, 655, 920, 1136, 1655, 2153
ApoI RAATTY 8 cut(s) 263, 298, 430, 492, 1120, 1142, 1190, 1567
AseI ATTAAT 2 cut(s) 630, 1206
Asp700I GAANNNNTTC 2 cut(s) 431, 1280
AspA2I CCTAGG 1 cut(s) 2203
AspLEI GCGC 1 cut(s) 1066
AspS9I GGNCC 2 cut(s) 1299, 2230
AsuC2I CCSGG 2 cut(s) 1674, 2121
AvaII GGWCC 2 cut(s) 1299, 2230
AvrII CCTAGG 1 cut(s) 2203
BaeGI GKGCMC 2 cut(s) 45, 1810
BaeI ACNNNNGTAYC 4 cut(s) 78, 111, 701, 734
BalI TGGCCA 2 cut(s) 372, 1958
BanII GRGCYC 1 cut(s) 2259
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
BauI CACGAG 1 cut(s) 194
BbsI GAAGAC 2 cut(s) 1156, 1835
Bbv12I GWGCWC 1 cut(s) 1358
BbvI GCAGC 6 cut(s) 226, 667, 932, 1148, 1667, 2140
BccI CCATC 5 cut(s) 172, 536, 841, 884, 1967
BciT130I CCWGG 2 cut(s) 736, 2060
BclI TGATCA 3 cut(s) 69, 660, 759
BcnI CCSGG 2 cut(s) 1674, 2121
BcoDI GTCTC 2 cut(s) 469, 984
BfaI CTAG 5 cut(s) 20, 1877, 2186, 2204, 2260
BfmI CTRYAG 3 cut(s) 771, 1470, 1641
BfoI RGCGCY 1 cut(s) 1067
BfrI CTTAAG 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 2077
BglII AGATCT 1 cut(s) 1727
BisI GCNGC 8 cut(s) 12, 240, 462, 656, 921, 1137, 1656, 2154
BlnI CCTAGG 1 cut(s) 2203
BlsI GCNGC 8 cut(s) 13, 241, 463, 657, 922, 1138, 1657, 2155
Bme1390I CCNGG 4 cut(s) 736, 1674, 2060, 2121
Bme18I GGWCC 2 cut(s) 1299, 2230
BmgT120I GGNCC 2 cut(s) 1299, 2230
BmiI GGNNCC 1 cut(s) 1098
BmrFI CCNGG 4 cut(s) 736, 1674, 2060, 2121
BmsI GCATC 2 cut(s) 1040, 1517
BpiI GAAGAC 2 cut(s) 1156, 1835
BplI GAGNNNNNCTC 2 cut(s) 505, 537
BpmI CTGGAG 1 cut(s) 1675
BpuEI CTTGAG 1 cut(s) 320
BpuMI CCSGG 2 cut(s) 1674, 2121
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 8 cut(s) 6, 90, 436, 1222, 1934, 1947, 2059, 2203
BsaWI WCCGGW 1 cut(s) 1555
BsaXI ACNNNNNCTCC 4 cut(s) 2088, 2115, 2118, 2145
Bsc4I CCNNNNNNNGG 3 cut(s) 1703, 1865, 2120
Bse118I RCCGGY 1 cut(s) 1415
Bse1I ACTGG 2 cut(s) 1623, 1859
Bse3DI GCAATG 1 cut(s) 809
BseBI CCWGG 2 cut(s) 736, 2060
BseDI CCNNGG 8 cut(s) 6, 90, 436, 1222, 1934, 1947, 2059, 2203
BseGI GGATG 3 cut(s) 306, 1192, 1508
BseLI CCNNNNNNNGG 3 cut(s) 1703, 1865, 2120
BseMI GCAATG 1 cut(s) 809
BseMII CTCAG 3 cut(s) 317, 381, 720
BseNI ACTGG 2 cut(s) 1623, 1859
BseSI GKGCMC 2 cut(s) 45, 1810
BseXI GCAGC 6 cut(s) 226, 667, 932, 1148, 1667, 2140
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 6 cut(s) 5, 11, 372, 734, 1044, 1958
BsiHKAI GWGCWC 1 cut(s) 1358
BsiSI CCGG 5 cut(s) 1416, 1556, 1674, 2072, 2121
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 3 cut(s) 1703, 1865, 2120
BsmAI GTCTC 2 cut(s) 469, 984
BsmFI GGGAC 1 cut(s) 315
BsnI GGCC 6 cut(s) 5, 11, 372, 734, 1044, 1958
Bsp1286I GDGCHC 4 cut(s) 45, 1358, 1810, 2259
Bsp1407I TGTACA 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 1222
BspACI CCGC 6 cut(s) 12, 282, 413, 461, 609, 832
BspANI GGCC 6 cut(s) 5, 11, 372, 734, 1044, 1958
BspCNI CTCAG 3 cut(s) 318, 382, 721
BspHI TCATGA 1 cut(s) 1699
BspLI GGNNCC 1 cut(s) 1098
BspMAI CTGCAG 2 cut(s) 775, 1474
BspPI GGATC 5 cut(s) 209, 665, 2047, 2050, 2254
BspTI CTTAAG 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 809
BsrFI RCCGGY 1 cut(s) 1415
BsrGI TGTACA 1 cut(s) 231
BsrI ACTGG 2 cut(s) 1623, 1859
BssAI RCCGGY 1 cut(s) 1415
BssECI CCNNGG 8 cut(s) 6, 90, 436, 1222, 1934, 1947, 2059, 2203
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 1 cut(s) 194
BssT1I CCWWGG 6 cut(s) 90, 436, 1222, 1934, 1947, 2203
Bst2BI CACGAG 1 cut(s) 194
Bst2UI CCWGG 2 cut(s) 736, 2060
Bst4CI ACNGT 5 cut(s) 410, 1575, 1645, 1984, 2017
Bst6I CTCTTC 2 cut(s) 2039, 2115
BstACI GRCGYC 1 cut(s) 53
BstAFI CTTAAG 1 cut(s) 422
BstAUI TGTACA 1 cut(s) 231
BstC8I GCNNGC 5 cut(s) 144, 282, 1906, 1941, 2069
BstDEI CTNAG 5 cut(s) 326, 390, 729, 884, 1922
BstDSI CCRYGG 1 cut(s) 1222
BstENI CCTNNNNNAGG 1 cut(s) 1701
BstF5I GGATG 3 cut(s) 306, 1192, 1508
BstH2I RGCGCY 1 cut(s) 1067
BstHHI GCGC 1 cut(s) 1066
BstMAI GTCTC 2 cut(s) 469, 984
BstMWI GCNNNNNNNGC 9 cut(s) 11, 139, 148, 190, 731, 1427, 1901, 2077, 2153
BstNI CCWGG 2 cut(s) 736, 2060
BstNSI RCATGY 3 cut(s) 818, 1265, 1445
BstSCI CCNGG 4 cut(s) 734, 1672, 2058, 2119
BstSFI CTRYAG 3 cut(s) 771, 1470, 1641
BstSLI GKGCMC 2 cut(s) 45, 1810
BstV1I GCAGC 6 cut(s) 226, 667, 932, 1148, 1667, 2140
BstV2I GAAGAC 2 cut(s) 1156, 1835
BstX2I RGATCY 1 cut(s) 1727
BstYI RGATCY 1 cut(s) 1727
BsuRI GGCC 6 cut(s) 5, 11, 372, 734, 1044, 1958
BtgI CCRYGG 1 cut(s) 1222
BtsCI GGATG 3 cut(s) 306, 1192, 1508
BtsI GCAGTG 2 cut(s) 366, 444
BtsIMutI CAGTG 4 cut(s) 366, 444, 1650, 1866
Cac8I GCNNGC 5 cut(s) 144, 282, 1906, 1941, 2069
CciI TCATGA 1 cut(s) 1699
CfoI GCGC 1 cut(s) 1066
Cfr10I RCCGGY 1 cut(s) 1415
Cfr13I GGNCC 2 cut(s) 1299, 2230
CseI GACGC 4 cut(s) 42, 912, 1932, 2076
Csp6I GTAC 2 cut(s) 232, 1919
CviQI GTAC 2 cut(s) 232, 1919
DdeI CTNAG 5 cut(s) 326, 390, 729, 884, 1922
DraI TTTAAA 1 cut(s) 1089
DraIII CACNNNGTG 1 cut(s) 49
EaeI YGGCCR 3 cut(s) 3, 370, 1956
Eam1104I CTCTTC 2 cut(s) 2039, 2115
EarI CTCTTC 2 cut(s) 2039, 2115
Eco130I CCWWGG 6 cut(s) 90, 436, 1222, 1934, 1947, 2203
Eco24I GRGCYC 1 cut(s) 2259
Eco47I GGWCC 2 cut(s) 1299, 2230
Eco47III AGCGCT 1 cut(s) 1065
EcoNI CCTNNNNNAGG 1 cut(s) 1701
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 2 cut(s) 734, 2058
EcoT14I CCWWGG 6 cut(s) 90, 436, 1222, 1934, 1947, 2203
EcoT38I GRGCYC 1 cut(s) 2259
ErhI CCWWGG 6 cut(s) 90, 436, 1222, 1934, 1947, 2203
FaqI GGGAC 1 cut(s) 315
FauI CCCGC 1 cut(s) 289
FauNDI CATATG 2 cut(s) 483, 570
FbaI TGATCA 3 cut(s) 69, 660, 759
FblI GTMKAC 2 cut(s) 1552, 2199
Fnu4HI GCNGC 8 cut(s) 12, 240, 462, 656, 921, 1137, 1656, 2154
FokI GGATG 3 cut(s) 293, 1199, 1495
FriOI GRGCYC 1 cut(s) 2259
Fsp4HI GCNGC 8 cut(s) 12, 240, 462, 656, 921, 1137, 1656, 2154
FspBI CTAG 5 cut(s) 20, 1877, 2186, 2204, 2260
GlaI GCGC 1 cut(s) 1065
GluI GCNGC 8 cut(s) 12, 240, 462, 656, 921, 1137, 1656, 2154
GsuI CTGGAG 1 cut(s) 1675
HaeII RGCGCY 1 cut(s) 1067
HaeIII GGCC 6 cut(s) 5, 11, 372, 734, 1044, 1958
HapII CCGG 5 cut(s) 1416, 1556, 1674, 2072, 2121
HgaI GACGC 4 cut(s) 42, 912, 1932, 2076
HhaI GCGC 1 cut(s) 1066
Hin1I GRCGYC 1 cut(s) 53
Hin6I GCGC 1 cut(s) 1064
HinP1I GCGC 1 cut(s) 1064
HincII GTYRAC 5 cut(s) 106, 323, 1051, 1609, 2200
HindII GTYRAC 5 cut(s) 106, 323, 1051, 1609, 2200
HindIII AAGCTT 1 cut(s) 1545
HinfI GANTC 4 cut(s) 157, 328, 977, 1465
HpaII CCGG 5 cut(s) 1416, 1556, 1674, 2072, 2121
Hpy166II GTNNAC 6 cut(s) 106, 323, 1051, 1553, 1609, 2200
Hpy188III TCNNGA 9 cut(s) 73, 337, 638, 664, 1035, 1340, 1700, 2021, 2186
Hpy8I GTNNAC 6 cut(s) 106, 323, 1051, 1553, 1609, 2200
HpyAV CCTTC 9 cut(s) 635, 684, 890, 935, 1079, 1106, 1290, 1697, 2168
HpyCH4III ACNGT 5 cut(s) 410, 1575, 1645, 1984, 2017
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 139, 148, 190, 731, 1427, 1901, 2077, 2153
HpyF3I CTNAG 5 cut(s) 326, 390, 729, 884, 1922
Hsp92I GRCGYC 1 cut(s) 53
HspAI GCGC 1 cut(s) 1064
Ksp22I TGATCA 3 cut(s) 69, 660, 759
LmnI GCTCC 2 cut(s) 1071, 1781
Lsp1109I GCAGC 6 cut(s) 226, 667, 932, 1148, 1667, 2140
LweI GCATC 2 cut(s) 1040, 1517
MaeI CTAG 5 cut(s) 20, 1877, 2186, 2204, 2260
MaeIII GTNAC 6 cut(s) 440, 532, 635, 1645, 1792, 2050
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 1727
MhlI GDGCHC 4 cut(s) 45, 1358, 1810, 2259
MlsI TGGCCA 2 cut(s) 372, 1958
MluNI TGGCCA 2 cut(s) 372, 1958
MlyI GAGTC 3 cut(s) 337, 986, 1474
MmeI TCCRAC 3 cut(s) 561, 1341, 1779
Mox20I TGGCCA 2 cut(s) 372, 1958
MroXI GAANNNNTTC 2 cut(s) 431, 1280
MscI TGGCCA 2 cut(s) 372, 1958
MseI TTAA 8 cut(s) 423, 506, 630, 753, 1088, 1206, 1307, 1542
MslI CAYNNNNRTG 6 cut(s) 198, 359, 642, 1260, 1440, 1766
Msp20I TGGCCA 2 cut(s) 372, 1958
MspA1I CMGCKG 2 cut(s) 658, 1658
MspCI CTTAAG 1 cut(s) 422
MspI CCGG 5 cut(s) 1416, 1556, 1674, 2072, 2121
MspR9I CCNGG 4 cut(s) 736, 1674, 2060, 2121
MunI CAATTG 1 cut(s) 747
MvaI CCWGG 2 cut(s) 736, 2060
MwoI GCNNNNNNNGC 9 cut(s) 11, 139, 148, 190, 731, 1427, 1901, 2077, 2153
NciI CCSGG 2 cut(s) 1674, 2121
NcoI CCATGG 1 cut(s) 1222
NdeI CATATG 2 cut(s) 483, 570
NlaIV GGNNCC 1 cut(s) 1098
NmeAIII GCCGAG 2 cut(s) 31, 40
NmuCI GTSAC 4 cut(s) 440, 635, 1645, 2050
NspI RCATGY 3 cut(s) 818, 1265, 1445
OliI CACNNNNGTG 1 cut(s) 642
PagI TCATGA 1 cut(s) 1699
PdmI GAANNNNTTC 2 cut(s) 431, 1280
PfeI GAWTC 1 cut(s) 157
PflFI GACNNNGTC 1 cut(s) 1604
PkrI GCNGC 8 cut(s) 13, 241, 463, 657, 922, 1138, 1657, 2155
PleI GAGTC 3 cut(s) 336, 985, 1473
PpsI GAGTC 3 cut(s) 336, 985, 1473
PshBI ATTAAT 2 cut(s) 630, 1206
PsiI TTATAA 2 cut(s) 1683, 2007
Psp6I CCWGG 2 cut(s) 734, 2058
PspGI CCWGG 2 cut(s) 734, 2058
PspN4I GGNNCC 1 cut(s) 1098
PspPI GGNCC 2 cut(s) 1299, 2230
PstI CTGCAG 2 cut(s) 775, 1474
PsuI RGATCY 1 cut(s) 1727
PsyI GACNNNGTC 1 cut(s) 1604
PvuII CAGCTG 2 cut(s) 658, 1658
RsaI GTAC 2 cut(s) 233, 1920
RsaNI GTAC 2 cut(s) 232, 1919
RseI CAYNNNNRTG 6 cut(s) 198, 359, 642, 1260, 1440, 1766
SalI GTCGAC 1 cut(s) 2198
SaqAI TTAA 8 cut(s) 423, 506, 630, 753, 1088, 1206, 1307, 1542
SatI GCNGC 8 cut(s) 12, 240, 462, 656, 921, 1137, 1656, 2154
Sau96I GGNCC 2 cut(s) 1299, 2230
SchI GAGTC 3 cut(s) 337, 986, 1474
ScrFI CCNGG 4 cut(s) 736, 1674, 2060, 2121
SduI GDGCHC 4 cut(s) 45, 1358, 1810, 2259
SfaNI GCATC 2 cut(s) 1040, 1517
SfcI CTRYAG 3 cut(s) 771, 1470, 1641
SinI GGWCC 2 cut(s) 1299, 2230
SmiMI CAYNNNNRTG 6 cut(s) 198, 359, 642, 1260, 1440, 1766
SmlI CTYRAG 2 cut(s) 335, 422
SmoI CTYRAG 2 cut(s) 335, 422
SsiI CCGC 6 cut(s) 12, 282, 413, 461, 609, 832
SspI AATATT 2 cut(s) 116, 1280
SspMI CTAG 5 cut(s) 20, 1877, 2186, 2204, 2260
StyD4I CCNGG 4 cut(s) 734, 1672, 2058, 2119
StyI CCWWGG 6 cut(s) 90, 436, 1222, 1934, 1947, 2203
TaaI ACNGT 5 cut(s) 410, 1575, 1645, 1984, 2017
TaqI TCGA 1 cut(s) 2199
TaqII GACCGA 1 cut(s) 683
TatI WGTACW 1 cut(s) 231
TauI GCSGC 2 cut(s) 14, 464
TfiI GAWTC 1 cut(s) 157
Tru1I TTAA 8 cut(s) 423, 506, 630, 753, 1088, 1206, 1307, 1542
Tru9I TTAA 8 cut(s) 423, 506, 630, 753, 1088, 1206, 1307, 1542
TscAI CASTG 4 cut(s) 366, 451, 1650, 1866
TseFI GTSAC 4 cut(s) 440, 635, 1645, 2050
TseI GCWGC 6 cut(s) 239, 655, 920, 1136, 1655, 2153
Tsp45I GTSAC 4 cut(s) 440, 635, 1645, 2050
TspGWI ACGGA 4 cut(s) 46, 89, 1512, 1768
TspRI CASTG 4 cut(s) 366, 451, 1650, 1866
Tth111I GACNNNGTC 1 cut(s) 1604
Vha464I CTTAAG 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 1299, 2230
VspI ATTAAT 2 cut(s) 630, 1206
XagI CCTNNNNNAGG 1 cut(s) 1701
XapI RAATTY 8 cut(s) 263, 298, 430, 492, 1120, 1142, 1190, 1567
XbaI TCTAGA 1 cut(s) 2185
XceI RCATGY 3 cut(s) 818, 1265, 1445
XmaJI CCTAGG 1 cut(s) 2203
XmiI GTMKAC 2 cut(s) 1552, 2199
XmnI GAANNNNTTC 2 cut(s) 431, 1280
XspI CTAG 5 cut(s) 20, 1877, 2186, 2204, 2260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.