Rh5AG275800

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000494
Physical Location & Seq
Forward (+)
36962217 .. 36964896
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG275800.1

Sequence Viewer

Length: 2175 bp
ATGCATCAAAATCAAATCCAGGACAGTCATCTAACCGATCACTCAAAATTGTACCATCAGTTGTACAGGTATGCTAGTGAGGGAAGGCAAACTGACTTTATCACAGCTATTCAGAAGGTTGCAAATTGTGATAAAAATGCTTCGATTCAGCTCCTGTCCCGACTGAGCCCGCTGAATAACTCATTTCTTCACACAGCAGTGACTTTCGGGCATGTAAGTCTAGCCATCAAGATCATGAAGCTTCACCCGCCACTTTTGTTGGTTAGAAACTTTGAAGGTGACACTGCACTCCACGTTGCGGCCAAAACCGGAGGGGGTGATGATAACCTGATTAGGTGCATTATGGAGCATGTGAATCTGGATTCTGGTGAAGGTCAAGCGACGGACATTGAAAAGGATGATATATCAGTGTTCAGAATGAAAAATGATGAGGAAAATACTCCCTTGCATGAGGCCCTGATAAATGGTAATCACTTGGAGGCAAAGTATTTGATCAAGGTTGATCCTTCAGTTTCATTTTGTGCTAACAAGGAGAGAAAGTCCCCTTTGTATCTAGCTGCAGAAGCTGGGCTCGTTGAAATTGTGAAGCTAATAAATGACGCTACTGCAGTTCAGAATAACACAGGTGATCAAGGTAATGGCGTGCCGCCTATGAACTTGGTGATGGAGGGCAAGTCCCCGTTGCATGCTGCGATTCTCAGCCAGAAGGAAGATGTCCTAGGCATAATATCAAGAATGCGATTTACCATAGAGCATTTCGAAGACGAGAAAGGGAGGACTCCACTTCATTGTGCAGCATCAACAGGTTATCTCGAAGGGCTTCGCTTCCTATTAAAAATATGTTTATCCGATTCTCATCGAGCAGATAGTGGTGGCACCTTTCCAATCCATTCTGCATCAAGCAAAGGTCATGTTCCTATAGTTAAGCTGCTTCTTGAACATTATCCCGATTTAAAGGAATTGAGGAACTCGAGTGGAGAAAACATTCTTCATGTTGCAGCAAAGTGTGGCAGGGACAATCTTGTGAAATATTTTCTCGAGAAAGGTGAATTTAAGATGTTGATAAACCAAAAAAACAAAGCTGGAAATACTCCTTTACATTTGGCTACAACTTACCAACATCCAGCAGTTGTGAACATCCTGACTTGGGATAAAAGGACCAATTTGAAGCTCTTGAATGTAAGAGGCATGACAGCTCTTGATATTGCAGAAAATACTCTGGAAACTACTGCATCGTTCCACGGGAGGCTAGCATGGATAGCACTGAAATCAGCGGGTGCACAGCAAGCTCAAAGTTCGAATATTCCACTAAGGAAAACAGGACAGAATACACAGCAAGAGTTGTTAGTTTCGGCAACTAAACCACTAGGAGGCAACGGTGGAGATGTCAGAACTAAAATTGAAGATGCTACGGACAGAGTTATAAATTCTTGTAACAATATTCAGCTAGTGTTACCAAACAAGCAAAGCAATAAGGACACAGTCAATACTCTTTTGGTGGTGACTACACTCATTGCCACAGTGTCGTTTGCAGCTGGTTTCACAATGCCAGGCGGCTACAATAACTCCGGCCCTCATGAGGGCACGGCAACTTTGCTAAATAAAGAAATGTTCCAAGTCTTCGTGATCTGCAACACACTTGCTATGTATAATTCTGTCCTTGTTGCAATCACTCTCATTTGGGCACAACTAGGGAATTTAAGCTTGGTGCCTCTTCGTATAGCATTGCCACTGTTGGGGCTCGCGCTTACAATGCTGTCCTTAGCATTCATGGCTGGAGTTTTTGTAGTAGTTAGTAGTCTTCAGTGGCTAGCTAGCGTTGTGATAATCATGGGTGTAGTTTTCCTTTTCACCGTGATAGCACTATTCACTCCACTCTTCTTCCCTTCCAAACTGTTCCCAACTACCTCCACTTTCCGTGTGATAACTTCTCTAAAATACATTGCAAATGAATATCCTGGAGGGATTGTGCACATCTTATATATGATCCCAAGCACTTCAGCCGAGCTAGACGTGTTGAACACCAGAGTAATCTCGTGCCGTCCTTACAATCCAGAGCCTAAGGACGGGACGGGATTAGTGGGAGCCACCAAAGGAGTTGTTAGACTCGGCCTTGGAGAAAAGCCCAGAGGGCTTGCCGGACTGTATTCATTAGGTTTAGTCCAAGCAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04310 map04310
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

724

Amino Acids

79.0

Weight (kDa)

8.52

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 140 - 199 1.2e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 220 - 279 6.3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 288 - 350 2.3e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 296 - 385 1.8e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 296 - 346 2.8e-06 Ankyrin repeats (many copies)
PGG PF13962 490 - 597 5.5e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03670 AT4G03440 AT4G03440 AT4G03440 AT4G03450 AT4G03450 AT4G03460 AT4G03460 AT4G03470 AT4G03480 AT4G03480 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03500 AT4G03500 AT4G03500 AT4G03505 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G14390 AT4G14390 AT4G14390 AT4G14390 AT4G14400 AT4G14400 AT4G14400
fragaria_vesca FvH4_3g05910 FvH4_3g23320
malus_domestica MD03G1094000.v1.1 MD03G1094200.v1.1 MD03G1094600.v1.1 MD12G1218700.v1.1
prunus_persica Prupe.3G135100_v2.0.a1 Prupe.3G135200_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G142400_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G154000_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154600_v2.0.a1 Prupe.3G154700_v2.0.a1 Prupe.3G155200_v2.0.a1 Prupe.3G155300_v2.0.a1 Prupe.8G110300_v2.0.a1
pyrus_communis pycom03g07280 pycom03g07580 pycom03g07660 pycom03g07700 pycom03g07710
rosa_chinensis RchiOBHm_Chr3g0461161 RchiOBHm_Chr5g0041111
rosa_laevigata RLG00000023472 RLG00000023475 RLG00000034025
rosa_multiflora Rmu_co8126040.1_g000001 Rmu_co8268563.1_g000001 Rmu_sc0000964.1_g000014 Rmu_sc0028923.1_g000001 Rmu_sc0042194.1_g000001
rosa_roxburghii Rroxscaffold_1G00039610 Rroxscaffold_6G00401360
rosa_rugosa Rorug03G0048600 Rorug03G0048700 Rorug05G0190000
rosa_samantha Rh3AG105900 Rh3BG110200 Rh3BG268400 Rh3CG111800 Rh3DG111900 Rh5AG275800 Rh5BG280500 Rh5CG312600 Rh5DG288800
rosa_wichuraiana Rw3G009080 Rw5G025920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1424
AccB1I GGYRCC 2 cut(s) 875, 1708
AccII CGCG 1 cut(s) 1745
AciI CCGC 6 cut(s) 170, 248, 299, 647, 1274, 1554
AclWI GGATC 2 cut(s) 497, 1981
AcoI YGGCCR 1 cut(s) 300
AcsI RAATTY 3 cut(s) 1049, 1426, 1696
AcuI CTGAAG 3 cut(s) 492, 1787, 1983
AfaI GTAC 2 cut(s) 53, 65
AfiI CCNNNNNNNGG 7 cut(s) 298, 1147, 1370, 1579, 1580, 1736, 2066
AflIII ACRYGT 1 cut(s) 2013
AgsI TTSAA 8 cut(s) 275, 392, 578, 938, 1168, 1177, 1403, 2020
AjiI CACGTC 1 cut(s) 2014
AjnI CCWGG 3 cut(s) 18, 1549, 1957
AjuI GAANNNNNNNTTGG 2 cut(s) 1688, 1720
AleI CACNNNNGTG 1 cut(s) 197
Alw21I GWGCWC 2 cut(s) 1282, 1974
Alw44I GTGCAC 2 cut(s) 1278, 1970
AlwI GGATC 2 cut(s) 497, 1981
AlwNI CAGNNNCTG 2 cut(s) 154, 566
Ama87I CYCGRG 2 cut(s) 970, 1037
AoxI GGCC 4 cut(s) 300, 453, 1570, 2110
ApaLI GTGCAC 2 cut(s) 1278, 1970
ApeKI GCWGC 6 cut(s) 557, 689, 794, 928, 998, 1532
ApoI RAATTY 3 cut(s) 1049, 1426, 1696
Asp700I GAANNNNTTC 2 cut(s) 819, 984
AspA2I CCTAGG 1 cut(s) 718
AspLEI GCGC 1 cut(s) 1747
AspS9I GGNCC 3 cut(s) 454, 1158, 1571
AsuHPI GGTGA 9 cut(s) 236, 290, 329, 380, 638, 673, 1058, 1513, 1843
AsuII TTCGAA 2 cut(s) 759, 1298
AsuNHI GCTAGC 3 cut(s) 1249, 1810, 1814
AvaI CYCGRG 2 cut(s) 970, 1037
AvaII GGWCC 1 cut(s) 1158
AvrII CCTAGG 1 cut(s) 718
AxyI CCTNAGG 1 cut(s) 2061
BaeGI GKGCMC 4 cut(s) 1282, 1586, 1687, 1974
BanI GGYRCC 2 cut(s) 875, 1708
BanII GRGCYC 3 cut(s) 170, 573, 1743
BauI CACGAG 1 cut(s) 2035
BbsI GAAGAC 3 cut(s) 768, 1612, 1793
Bbv12I GWGCWC 2 cut(s) 1282, 1974
BbvI GCAGC 6 cut(s) 544, 676, 806, 915, 1010, 1544
BccI CCATC 3 cut(s) 63, 233, 658
BceAI ACGGC 2 cut(s) 1602, 2025
BciT130I CCWGG 3 cut(s) 20, 1551, 1959
BclI TGATCA 2 cut(s) 492, 628
BfmI CTRYAG 3 cut(s) 558, 606, 918
BglI GCCNNNNNGGC 1 cut(s) 2131
BisI GCNGC 9 cut(s) 300, 558, 647, 690, 795, 929, 999, 1533, 1555
BlnI CCTAGG 1 cut(s) 718
BlsI GCNGC 9 cut(s) 301, 559, 648, 691, 796, 930, 1000, 1534, 1556
Bme1390I CCNGG 3 cut(s) 20, 1551, 1959
Bme18I GGWCC 1 cut(s) 1158
BmeT110I CYCGRG 2 cut(s) 970, 1037
BmgBI CACGTC 1 cut(s) 2014
BmgT120I GGNCC 3 cut(s) 454, 1158, 1571
BmiI GGNNCC 3 cut(s) 877, 1710, 2086
BmrFI CCNGG 3 cut(s) 20, 1551, 1959
BmsI GCATC 5 cut(s) 13, 806, 905, 1241, 1396
BmtI GCTAGC 3 cut(s) 1253, 1814, 1818
BpiI GAAGAC 3 cut(s) 768, 1612, 1793
BpmI CTGGAG 2 cut(s) 1797, 1980
Bpu10I CCTNAGC 1 cut(s) 1762
Bpu14I TTCGAA 2 cut(s) 759, 1298
BsaBI GATNNNNATC 1 cut(s) 855
BsaJI CCNNGG 3 cut(s) 718, 1240, 2113
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 7 cut(s) 298, 1147, 1370, 1579, 1580, 1736, 2066
Bse21I CCTNAGG 1 cut(s) 2061
Bse3DI GCAATG 3 cut(s) 1512, 1724, 1941
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 3 cut(s) 20, 1551, 1959
BseDI CCNNGG 3 cut(s) 718, 1240, 2113
BseGI GGATG 3 cut(s) 403, 1120, 1137
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 7 cut(s) 298, 1147, 1370, 1579, 1580, 1736, 2066
BseMI GCAATG 3 cut(s) 1512, 1724, 1941
BseMII CTCAG 2 cut(s) 155, 712
BseSI GKGCMC 4 cut(s) 1282, 1586, 1687, 1974
BseXI GCAGC 6 cut(s) 544, 676, 806, 915, 1010, 1544
BseYI CCCAGC 1 cut(s) 566
BsgI GTGCAG 2 cut(s) 270, 813
Bsh1236I CGCG 1 cut(s) 1745
BshFI GGCC 4 cut(s) 302, 455, 1572, 2112
BshNI GGYRCC 2 cut(s) 875, 1708
BsiHKAI GWGCWC 2 cut(s) 1282, 1974
BsiHKCI CYCGRG 2 cut(s) 970, 1037
BsiSI CCGG 3 cut(s) 309, 1569, 2139
BslFI GGGAC 5 cut(s) 142, 526, 661, 1028, 2083
BslI CCNNNNNNNGG 7 cut(s) 298, 1147, 1370, 1579, 1580, 1736, 2066
BsmFI GGGAC 5 cut(s) 142, 526, 661, 1028, 2083
BsmI GAATGC 2 cut(s) 741, 1766
BsnI GGCC 4 cut(s) 302, 455, 1572, 2112
BsoBI CYCGRG 2 cut(s) 970, 1037
Bsp119I TTCGAA 2 cut(s) 759, 1298
Bsp1286I GDGCHC 7 cut(s) 170, 573, 1282, 1586, 1687, 1743, 1974
Bsp1407I TGTACA 1 cut(s) 63
Bsp143I GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
BspACI CCGC 6 cut(s) 170, 248, 299, 647, 1274, 1554
BspANI GGCC 4 cut(s) 302, 455, 1572, 2112
BspCNI CTCAG 2 cut(s) 156, 711
BspFNI CGCG 1 cut(s) 1745
BspHI TCATGA 2 cut(s) 234, 1576
BspLI GGNNCC 3 cut(s) 877, 1710, 2086
BspMAI CTGCAG 2 cut(s) 562, 610
BspOI GCTAGC 3 cut(s) 1253, 1814, 1818
BspPI GGATC 2 cut(s) 497, 1981
BspT104I TTCGAA 2 cut(s) 759, 1298
BspT107I GGYRCC 2 cut(s) 875, 1708
BsrDI GCAATG 3 cut(s) 1512, 1724, 1941
BsrGI TGTACA 1 cut(s) 63
BssECI CCNNGG 3 cut(s) 718, 1240, 2113
BssMI GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
BssSI CACGAG 1 cut(s) 2035
BssT1I CCWWGG 2 cut(s) 718, 2113
Bst2BI CACGAG 1 cut(s) 2035
Bst2UI CCWGG 3 cut(s) 20, 1551, 1959
Bst4CI ACNGT 8 cut(s) 26, 1379, 1483, 1522, 1734, 1855, 1896, 2145
Bst6I CTCTTC 2 cut(s) 1719, 1883
BstAUI TGTACA 1 cut(s) 63
BstBI TTCGAA 2 cut(s) 759, 1298
BstC8I GCNNGC 9 cut(s) 170, 644, 687, 1251, 1287, 1743, 1812, 1816, 2136
BstDEI CTNAG 5 cut(s) 164, 698, 1310, 1762, 2061
BstDSI CCRYGG 1 cut(s) 1240
BstF5I GGATG 3 cut(s) 403, 1120, 1137
BstFNI CGCG 1 cut(s) 1745
BstHHI GCGC 1 cut(s) 1747
BstKTI GATC 7 cut(s) 40, 234, 495, 505, 631, 1629, 1989
BstMBI GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
BstMWI GCNNNNNNNGC 7 cut(s) 247, 563, 1259, 1286, 1753, 1772, 2131
BstNI CCWGG 3 cut(s) 20, 1551, 1959
BstNSI RCATGY 3 cut(s) 215, 353, 689
BstSCI CCNGG 3 cut(s) 18, 1549, 1957
BstSFI CTRYAG 3 cut(s) 558, 606, 918
BstSLI GKGCMC 4 cut(s) 1282, 1586, 1687, 1974
BstUI CGCG 1 cut(s) 1745
BstV1I GCAGC 6 cut(s) 544, 676, 806, 915, 1010, 1544
BstV2I GAAGAC 3 cut(s) 768, 1612, 1793
Bsu36I CCTNAGG 1 cut(s) 2061
BsuRI GGCC 4 cut(s) 302, 455, 1572, 2112
BtgI CCRYGG 1 cut(s) 1240
BtrI CACGTC 1 cut(s) 2014
BtsCI GGATG 3 cut(s) 403, 1120, 1137
BtsI GCAGTG 2 cut(s) 204, 282
BtsIMutI CAGTG 7 cut(s) 204, 282, 414, 1262, 1527, 1730, 1811
Cac8I GCNNGC 9 cut(s) 170, 644, 687, 1251, 1287, 1743, 1812, 1816, 2136
CaiI CAGNNNCTG 2 cut(s) 154, 566
CciI TCATGA 2 cut(s) 234, 1576
CfoI GCGC 1 cut(s) 1747
Cfr13I GGNCC 3 cut(s) 454, 1158, 1571
CseI GACGC 1 cut(s) 608
Csp6I GTAC 2 cut(s) 52, 64
CspCI CAANNNNNGTGG 2 cut(s) 240, 275
CviQI GTAC 2 cut(s) 52, 64
DdeI CTNAG 5 cut(s) 164, 698, 1310, 1762, 2061
DpnI GATC 7 cut(s) 39, 233, 494, 504, 630, 1628, 1988
DpnII GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
DraI TTTAAA 1 cut(s) 954
EaeI YGGCCR 1 cut(s) 300
Eam1104I CTCTTC 2 cut(s) 1719, 1883
EarI CTCTTC 2 cut(s) 1719, 1883
Eco130I CCWWGG 2 cut(s) 718, 2113
Eco24I GRGCYC 3 cut(s) 170, 573, 1743
Eco47I GGWCC 1 cut(s) 1158
Eco57I CTGAAG 3 cut(s) 492, 1787, 1983
Eco81I CCTNAGG 1 cut(s) 2061
Eco88I CYCGRG 2 cut(s) 970, 1037
EcoO109I RGGNCCY 1 cut(s) 454
EcoRII CCWGG 3 cut(s) 18, 1549, 1957
EcoT14I CCWWGG 2 cut(s) 718, 2113
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 170, 573, 1743
ErhI CCWWGG 2 cut(s) 718, 2113
FaqI GGGAC 5 cut(s) 142, 526, 661, 1028, 2083
FauI CCCGC 3 cut(s) 177, 255, 1267
FbaI TGATCA 2 cut(s) 492, 628
Fnu4HI GCNGC 9 cut(s) 300, 558, 647, 690, 795, 929, 999, 1533, 1555
FokI GGATG 3 cut(s) 410, 1107, 1124
FriOI GRGCYC 3 cut(s) 170, 573, 1743
Fsp4HI GCNGC 9 cut(s) 300, 558, 647, 690, 795, 929, 999, 1533, 1555
GlaI GCGC 1 cut(s) 1746
GluI GCNGC 9 cut(s) 300, 558, 647, 690, 795, 929, 999, 1533, 1555
GsaI CCCAGC 1 cut(s) 570
GsuI CTGGAG 2 cut(s) 1797, 1980
HaeIII GGCC 4 cut(s) 302, 455, 1572, 2112
HapII CCGG 3 cut(s) 309, 1569, 2139
HgaI GACGC 1 cut(s) 608
HhaI GCGC 1 cut(s) 1747
Hin6I GCGC 1 cut(s) 1745
HinP1I GCGC 1 cut(s) 1745
HindIII AAGCTT 2 cut(s) 239, 1702
HinfI GANTC 7 cut(s) 145, 355, 362, 694, 778, 851, 2106
HpaII CCGG 3 cut(s) 309, 1569, 2139
HphI GGTGA 9 cut(s) 236, 290, 329, 380, 638, 673, 1058, 1513, 1843
Hpy166II GTNNAC 3 cut(s) 1135, 1280, 1972
Hpy188I TCNGA 5 cut(s) 114, 416, 615, 850, 1391
Hpy8I GTNNAC 3 cut(s) 1135, 1280, 1972
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 8 cut(s) 78, 109, 269, 365, 516, 700, 809, 1896
HpyCH4III ACNGT 8 cut(s) 26, 1379, 1483, 1522, 1734, 1855, 1896, 2145
HpyCH4IV ACGT 2 cut(s) 294, 2013
HpyF10VI GCNNNNNNNGC 7 cut(s) 247, 563, 1259, 1286, 1753, 1772, 2131
HpyF3I CTNAG 5 cut(s) 164, 698, 1310, 1762, 2061
HpySE526I ACGT 2 cut(s) 294, 2013
HspAI GCGC 1 cut(s) 1745
Ksp22I TGATCA 2 cut(s) 492, 628
Kzo9I GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
LmnI GCTCC 3 cut(s) 156, 346, 2084
Lsp1109I GCAGC 6 cut(s) 544, 676, 806, 915, 1010, 1544
LweI GCATC 5 cut(s) 13, 806, 905, 1241, 1396
MaeII ACGT 2 cut(s) 294, 2013
MaeIII GTNAC 5 cut(s) 199, 278, 1433, 1452, 1501
MalI GATC 7 cut(s) 39, 233, 494, 504, 630, 1628, 1988
MboI GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
MhlI GDGCHC 7 cut(s) 170, 573, 1282, 1586, 1687, 1743, 1974
MlyI GAGTC 2 cut(s) 772, 2100
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 819, 984
MseI TTAA 5 cut(s) 833, 924, 953, 1053, 1700
MslI CAYNNNNRTG 1 cut(s) 197
MspA1I CMGCKG 3 cut(s) 172, 1274, 1535
MspI CCGG 3 cut(s) 309, 1569, 2139
MspR9I CCNGG 3 cut(s) 20, 1551, 1959
Mva1269I GAATGC 2 cut(s) 741, 1766
MvaI CCWGG 3 cut(s) 20, 1551, 1959
MvnI CGCG 1 cut(s) 1745
MwoI GCNNNNNNNGC 7 cut(s) 247, 563, 1259, 1286, 1753, 1772, 2131
NdeII GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
NheI GCTAGC 3 cut(s) 1249, 1810, 1814
NlaIV GGNNCC 3 cut(s) 877, 1710, 2086
NmeAIII GCCGAG 2 cut(s) 2029, 2088
NmuCI GTSAC 3 cut(s) 199, 278, 1501
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 215, 353, 689
NspV TTCGAA 2 cut(s) 759, 1298
OliI CACNNNNGTG 1 cut(s) 197
PaeI GCATGC 1 cut(s) 689
PaeR7I CTCGAG 2 cut(s) 970, 1037
PagI TCATGA 2 cut(s) 234, 1576
PctI GAATGC 2 cut(s) 741, 1766
PdmI GAANNNNTTC 2 cut(s) 819, 984
PfeI GAWTC 5 cut(s) 145, 355, 362, 694, 851
PflFI GACNNNGTC 1 cut(s) 1481
PfoI TCCNGGA 2 cut(s) 18, 1957
PkrI GCNGC 9 cut(s) 301, 559, 648, 691, 796, 930, 1000, 1534, 1556
PleI GAGTC 2 cut(s) 772, 2100
PpsI GAGTC 2 cut(s) 772, 2100
PsiI TTATAA 1 cut(s) 1424
Psp6I CCWGG 3 cut(s) 18, 1549, 1957
PspFI CCCAGC 1 cut(s) 566
PspGI CCWGG 3 cut(s) 18, 1549, 1957
PspN4I GGNNCC 3 cut(s) 877, 1710, 2086
PspPI GGNCC 3 cut(s) 454, 1158, 1571
PspXI VCTCGAGB 1 cut(s) 970
PstI CTGCAG 2 cut(s) 562, 610
PstNI CAGNNNCTG 2 cut(s) 154, 566
PsyI GACNNNGTC 1 cut(s) 1481
PvuII CAGCTG 1 cut(s) 1535
RsaI GTAC 2 cut(s) 53, 65
RsaNI GTAC 2 cut(s) 52, 64
RseI CAYNNNNRTG 1 cut(s) 197
SaqAI TTAA 5 cut(s) 833, 924, 953, 1053, 1700
SatI GCNGC 9 cut(s) 300, 558, 647, 690, 795, 929, 999, 1533, 1555
Sau3AI GATC 7 cut(s) 37, 231, 492, 502, 628, 1626, 1986
Sau96I GGNCC 3 cut(s) 454, 1158, 1571
SchI GAGTC 2 cut(s) 772, 2100
ScrFI CCNGG 3 cut(s) 20, 1551, 1959
SduI GDGCHC 7 cut(s) 170, 573, 1282, 1586, 1687, 1743, 1974
SfaNI GCATC 5 cut(s) 13, 806, 905, 1241, 1396
SfcI CTRYAG 3 cut(s) 558, 606, 918
Sfr274I CTCGAG 2 cut(s) 970, 1037
SfuI TTCGAA 2 cut(s) 759, 1298
SinI GGWCC 1 cut(s) 1158
SlaI CTCGAG 2 cut(s) 970, 1037
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 2 cut(s) 970, 1037
SmoI CTYRAG 2 cut(s) 970, 1037
SphI GCATGC 1 cut(s) 689
SsiI CCGC 6 cut(s) 170, 248, 299, 647, 1274, 1554
SspI AATATT 3 cut(s) 1031, 1303, 1441
StyD4I CCNGG 3 cut(s) 18, 1549, 1957
StyI CCWWGG 2 cut(s) 718, 2113
TaaI ACNGT 8 cut(s) 26, 1379, 1483, 1522, 1734, 1855, 1896, 2145
TaiI ACGT 2 cut(s) 297, 2016
TaqI TCGA 7 cut(s) 143, 759, 813, 859, 971, 1038, 1298
TatI WGTACW 1 cut(s) 63
TauI GCSGC 3 cut(s) 302, 649, 1557
TfiI GAWTC 5 cut(s) 145, 355, 362, 694, 851
Tru1I TTAA 5 cut(s) 833, 924, 953, 1053, 1700
Tru9I TTAA 5 cut(s) 833, 924, 953, 1053, 1700
TscAI CASTG 7 cut(s) 204, 289, 414, 1269, 1527, 1737, 1811
TseFI GTSAC 3 cut(s) 199, 278, 1501
TseI GCWGC 6 cut(s) 557, 689, 794, 928, 998, 1532
Tsp45I GTSAC 3 cut(s) 199, 278, 1501
TspDTI ATGAA 9 cut(s) 251, 434, 504, 668, 776, 980, 1759, 1965, 2139
TspGWI ACGGA 3 cut(s) 398, 1427, 1907
TspRI CASTG 7 cut(s) 204, 289, 414, 1269, 1527, 1737, 1811
Tth111I GACNNNGTC 1 cut(s) 1481
VneI GTGCAC 2 cut(s) 1278, 1970
VpaK11BI GGWCC 1 cut(s) 1158
XapI RAATTY 3 cut(s) 1049, 1426, 1696
XceI RCATGY 3 cut(s) 215, 353, 689
XhoI CTCGAG 2 cut(s) 970, 1037
XmaJI CCTAGG 1 cut(s) 718
XmnI GAANNNNTTC 2 cut(s) 819, 984
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.