Rw3G009080

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000494
Physical Location & Seq
Forward (+)
8589183 .. 8594378
5196 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G009080.1

Sequence Viewer

Length: 1968 bp
ATGGCTACTTCGAGTTCCACAAATGGAGTAATACAAGATGAAATAACTGTAAACCAAGCTGCAGAGGATCTCGAGGCTACAGGGGACAGACGAATGGATCCAAAATTATACAGGTATGTAACCACTGGTAAAGTAGACCAAAAAAGTGGTTTCCGGGCAATAATTACGGTCCCCTTCTGTAATCCTTTTTCGGTCTTTAGACAAATAGTAGAGGTCATGATCTACCAAAAAATCTTGGACTTTTACGAATGGCTTCTCCATATGTATACAACCTACTCTTATTCCGGCTTCAGTATTCCCTCTGAAGTAGAATTACTGTTACAAATAAATGGAGACAAAGTAAGCCCTGGCCGGAATACAGTGCTTCACCTTGCGGCAAGTGTAGGCAATACCCATCTCGTCACACACATACTGGATCGTTTTCCGAGCCATGTCACAGAAAACAACTGTCGTGGAGATTCTCCGCTTCATGCTGCAGCATACGGCGGGCATCTATCTACGGTGCGTTGTTTGGTTGAAAAGTTTAAGACACTGATTGGTGAAAAGAATGCGGAAGGAAACACAGCGTTACACCTTGCCTTAGCAAATCACCACAAAAAAGTGGCCAAGTTTTTAGTCGAACAAGATGGATTGATCTCGCATTCCCAAAATAACAGCAAAAAGACACCGTTGTGCATGGCCGCGGAAGCTGGGAATCTTGACCTTGTTCAAGTTATGGTGGTCATGACACCAGCTGACAGGGCTTCTGTTTGGGAAGAGTCCAGTGGCTACTCAATTTTGCGTGCTGCTATCGTGGGAAGGAATAATGACATCTTGAAGACATTATTAGTCAAAATTGATCCTACTCTCATCCATGTGTCAAAAGACGAAGAGGAAGAGGGGATGCCTCCTCTTGCACTTGCAGCATATATAGGTAACTTTGATGCCGTACGCTGCTTGTTAGACAAATACAGTAACGATGCATACAGGCAGGACACAAATGGATTCTTTCCGATATTTATAGCGTCCCGAAGCGGCAACATCAAGATAGTTCAAGAATTCCTCAATCATTGCCCAGACTTGGAGGAGTTACATGACAAGCAAGGCCGGAACATTCTTCATGTGGCAGCTGCGTATGGACGAGCTAAGCTGGTGGAATATATGCTTGGAACGACCAAGCTTGAAACGCTTCTCAACGAAAGAGATAATTTCGGAAACACCCCTCTACACATAGCTGTTCAGAACTGGCATCCTAGGATTGTCAAAATTCTGACCCGGGATAAGAGAGTTCACCAAAAGATTCTCAACAATAAGGGCATGACAGCATTCGATATTGCAGAGAAGTCCGAGGTGCATAAAGACATGCTGTTTCGATGGAAACTAACTTTTATGGCCTTGAGATTAGCTAATGCTCCCCGATCTGAGATACAAAGAGTTGCAAAAGAAAGTGGAGCAAATGATTCTGGTGAGAAGACCAAGAATAATCCTGATTATCTACTATTCTCAAGAGAAAATTTGAATACTCTCCTTATAGTGTCGACACTGGTGGCTGGTATAACTTTTGCTGCTGGCTTCACTGTGCCGGACGATCGCATGCTCTATGGTGATGCAGCTCTATCCCCAATATTCTTGATATGCAACACCATTTCAATGTACAGCTCTATTACCGTTGCGCTTGCGCTTATCTGGGCACAAACAGGTGATCTTCAAATGATTTGTGTTGCTGTGAGGACTATACTACCCATGTTAGGCCTAGCTCTTATAATGATGTCCGTGGCATTCCTAGCTGGTGTTGGTGTCGTAGCGAATAGCTATACTTGTGGCTACGTTGTCGTGGCCCTGGGCTCATTTTTCATCGTCCAAGTAGTTGTGTTCATTCTTCCGCTCACAATCCCACTTTCCTCCAAGAATTGCATTACACACTTCATTTTACGTGGTAACCTTCGTTTGCTGATGATGGTAACTAGATATGAGGTTGATAGTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0002831 GO:0002833 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009966 GO:0009987 GO:0010033 GO:0010646 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016567 GO:0019538 GO:0023051 GO:0030176 GO:0031224 GO:0031226 GO:0031227 GO:0031347 GO:0031349 GO:0031984 GO:0032101 GO:0032103 GO:0032446 GO:0032870 GO:0035690 GO:0036211 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045087 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0098827 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901700 GO:1901701 GO:1902288 GO:1902290 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

655

Amino Acids

72.7

Weight (kDa)

7.05

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 103 - 174 5.4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 122 - 208 1.3e-15 Ankyrin repeats (3 copies)
Ank_4 PF13637 156 - 206 1.6e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 267 - 350 1.6e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 298 - 382 1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 366 - 417 1.2e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 391 - 439 9.5e-06 Ankyrin repeats (many copies)
PGG PF13962 497 - 592 2.8e-13 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03670 AT4G03440 AT4G03440 AT4G03440 AT4G03450 AT4G03450 AT4G03460 AT4G03460 AT4G03470 AT4G03480 AT4G03480 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03500 AT4G03500 AT4G03500 AT4G03505 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G14390 AT4G14390 AT4G14390 AT4G14390 AT4G14400 AT4G14400 AT4G14400
fragaria_vesca FvH4_3g05910 FvH4_3g23320
malus_domestica MD03G1094000.v1.1 MD03G1094200.v1.1 MD03G1094600.v1.1 MD12G1218700.v1.1
prunus_persica Prupe.3G135100_v2.0.a1 Prupe.3G135200_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G142400_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G154000_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154600_v2.0.a1 Prupe.3G154700_v2.0.a1 Prupe.3G155200_v2.0.a1 Prupe.3G155300_v2.0.a1 Prupe.8G110300_v2.0.a1
pyrus_communis pycom03g07280 pycom03g07580 pycom03g07660 pycom03g07700 pycom03g07710
rosa_chinensis RchiOBHm_Chr3g0461161 RchiOBHm_Chr5g0041111
rosa_laevigata RLG00000023472 RLG00000023475 RLG00000034025
rosa_multiflora Rmu_co8126040.1_g000001 Rmu_co8268563.1_g000001 Rmu_sc0000964.1_g000014 Rmu_sc0028923.1_g000001 Rmu_sc0042194.1_g000001
rosa_roxburghii Rroxscaffold_1G00039610 Rroxscaffold_6G00401360
rosa_rugosa Rorug03G0048600 Rorug03G0048700 Rorug05G0190000
rosa_samantha Rh3AG105900 Rh3BG110200 Rh3BG268400 Rh3CG111800 Rh3DG111900 Rh5AG275800 Rh5BG280500 Rh5CG312600 Rh5DG288800
rosa_wichuraiana Rw3G009080 Rw5G025920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1742
AccBSI CCGCTC 1 cut(s) 1864
AccI GTMKAC 3 cut(s) 135, 266, 1517
AccII CGCG 1 cut(s) 683
AciI CCGC 8 cut(s) 374, 464, 486, 551, 681, 683, 1014, 1862
AclWI GGATC 5 cut(s) 75, 92, 105, 423, 833
AcoI YGGCCR 3 cut(s) 349, 603, 678
AcsI RAATTY 3 cut(s) 1037, 1245, 1492
AcuI CTGAAG 2 cut(s) 274, 324
AfaI GTAC 2 cut(s) 930, 1634
AfiI CCNNNNNNNGG 2 cut(s) 1060, 1727
AgsI TTSAA 8 cut(s) 518, 710, 817, 1034, 1163, 1498, 1629, 1688
AjnI CCWGG 2 cut(s) 346, 1818
AjuI GAANNNNNNNTTGG 2 cut(s) 1128, 1160
AleI CACNNNNGTG 1 cut(s) 670
Alw26I GTCTC 1 cut(s) 327
AlwI GGATC 5 cut(s) 75, 92, 105, 423, 833
Ama87I CYCGRG 2 cut(s) 71, 1254
AoxI GGCC 7 cut(s) 349, 603, 678, 1084, 1371, 1729, 1815
ApoI RAATTY 3 cut(s) 1037, 1245, 1492
Asp700I GAANNNNTTC 2 cut(s) 252, 1167
AspA2I CCTAGG 1 cut(s) 1232
AspLEI GCGC 2 cut(s) 1654, 1660
AspS9I GGNCC 2 cut(s) 169, 1816
AsuC2I CCSGG 3 cut(s) 155, 1255, 1256
AsuHPI GGTGA 7 cut(s) 359, 551, 581, 1262, 1457, 1595, 1691
AvaI CYCGRG 2 cut(s) 71, 1254
AvaII GGWCC 1 cut(s) 169
AvrII CCTAGG 1 cut(s) 1232
BaeGI GKGCMC 1 cut(s) 1672
BalI TGGCCA 1 cut(s) 605
BamHI GGATCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 1826
BarI GAAGNNNNNNTAC 2 cut(s) 297, 329
BbsI GAAGAC 2 cut(s) 824, 1457
BccI CCATC 4 cut(s) 402, 620, 1347, 1930
BceAI ACGGC 2 cut(s) 499, 911
BciT130I CCWGG 2 cut(s) 348, 1820
BcnI CCSGG 3 cut(s) 155, 1255, 1256
BcoDI GTCTC 1 cut(s) 327
BfaI CTAG 4 cut(s) 1233, 1733, 1763, 1944
BfmI CTRYAG 3 cut(s) 60, 78, 474
BlnI CCTAGG 1 cut(s) 1232
BlpI GCTNAGC 1 cut(s) 1125
Bme1390I CCNGG 5 cut(s) 155, 348, 1255, 1256, 1820
Bme18I GGWCC 1 cut(s) 169
BmeT110I CYCGRG 2 cut(s) 71, 1254
BmgT120I GGNCC 2 cut(s) 169, 1816
BmiI GGNNCC 2 cut(s) 99, 171
BmrFI CCNGG 5 cut(s) 155, 348, 1255, 1256, 1820
BmsI GCATC 6 cut(s) 499, 873, 913, 949, 1237, 1576
BpiI GAAGAC 2 cut(s) 824, 1457
Bpu10I CCTNAGC 1 cut(s) 580
Bpu1102I GCTNAGC 1 cut(s) 1125
BpuEI CTTGAG 2 cut(s) 1396, 1468
BpuMI CCSGG 3 cut(s) 155, 1255, 1256
BsaAI YACGTR 1 cut(s) 1913
BsaJI CCNNGG 8 cut(s) 346, 681, 1232, 1254, 1326, 1752, 1818, 1819
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 1060, 1727
Bse1I ACTGG 5 cut(s) 130, 417, 762, 1229, 1527
Bse3DI GCAATG 1 cut(s) 1048
BseBI CCWGG 2 cut(s) 348, 1820
BseDI CCNNGG 8 cut(s) 346, 681, 1232, 1254, 1326, 1752, 1818, 1819
BseGI GGATG 3 cut(s) 849, 888, 1228
BseLI CCNNNNNNNGG 2 cut(s) 1060, 1727
BseMI GCAATG 1 cut(s) 1048
BseMII CTCAG 1 cut(s) 1392
BseNI ACTGG 5 cut(s) 130, 417, 762, 1229, 1527
BseRI GAGGAG 2 cut(s) 879, 1079
BseSI GKGCMC 1 cut(s) 1672
BseYI CCCAGC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 683
Bsh1285I CGRYCG 1 cut(s) 1570
BshFI GGCC 7 cut(s) 351, 605, 680, 1086, 1373, 1731, 1817
BsiEI CGRYCG 1 cut(s) 1570
BsiHKCI CYCGRG 2 cut(s) 71, 1254
BsiSI CCGG 6 cut(s) 154, 285, 352, 1087, 1255, 1562
BsiWI CGTACG 1 cut(s) 928
BslFI GGGAC 3 cut(s) 98, 155, 991
BslI CCNNNNNNNGG 2 cut(s) 1060, 1727
BsmAI GTCTC 1 cut(s) 327
BsmFI GGGAC 3 cut(s) 98, 155, 991
BsmI GAATGC 4 cut(s) 553, 640, 1304, 1757
BsnI GGCC 7 cut(s) 351, 605, 680, 1086, 1373, 1731, 1817
BsoBI CYCGRG 2 cut(s) 71, 1254
Bsp1286I GDGCHC 2 cut(s) 1672, 1826
Bsp1407I TGTACA 1 cut(s) 1632
Bsp143I GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
Bsp1720I GCTNAGC 1 cut(s) 1125
BspACI CCGC 8 cut(s) 374, 464, 486, 551, 681, 683, 1014, 1862
BspANI GGCC 7 cut(s) 351, 605, 680, 1086, 1373, 1731, 1817
BspCNI CTCAG 1 cut(s) 1393
BspFNI CGCG 1 cut(s) 683
BspHI TCATGA 2 cut(s) 216, 723
BspLI GGNNCC 2 cut(s) 99, 171
BspMAI CTGCAG 2 cut(s) 64, 478
BspPI GGATC 5 cut(s) 75, 92, 105, 423, 833
BsrBI CCGCTC 1 cut(s) 1864
BsrDI GCAATG 1 cut(s) 1048
BsrGI TGTACA 1 cut(s) 1632
BsrI ACTGG 5 cut(s) 130, 417, 762, 1229, 1527
BssECI CCNNGG 8 cut(s) 346, 681, 1232, 1254, 1326, 1752, 1818, 1819
BssMI GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
BssNAI GTATAC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 1232
Bst1107I GTATAC 1 cut(s) 267
Bst2UI CCWGG 2 cut(s) 348, 1820
Bst6I CTCTTC 3 cut(s) 750, 864, 870
BstAUI TGTACA 1 cut(s) 1632
BstBAI YACGTR 1 cut(s) 1913
BstC8I GCNNGC 5 cut(s) 488, 783, 1549, 1574, 1656
BstDEI CTNAG 3 cut(s) 580, 1125, 1401
BstDSI CCRYGG 2 cut(s) 681, 1752
BstEII GGTNACC 1 cut(s) 1916
BstF5I GGATG 3 cut(s) 849, 888, 1228
BstFNI CGCG 1 cut(s) 683
BstHHI GCGC 2 cut(s) 1654, 1660
BstKTI GATC 9 cut(s) 70, 100, 222, 418, 636, 841, 1400, 1570, 1684
BstMAI GTCTC 1 cut(s) 327
BstMBI GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
BstMCI CGRYCG 1 cut(s) 1570
BstMWI GCNNNNNNNGC 5 cut(s) 686, 740, 902, 1165, 1763
BstNI CCWGG 2 cut(s) 348, 1820
BstNSI RCATGY 2 cut(s) 1345, 1576
BstPI GGTNACC 1 cut(s) 1916
BstSCI CCNGG 5 cut(s) 153, 346, 1253, 1254, 1818
BstSFI CTRYAG 3 cut(s) 60, 78, 474
BstSLI GKGCMC 1 cut(s) 1672
BstUI CGCG 1 cut(s) 683
BstV2I GAAGAC 2 cut(s) 824, 1457
BstX2I RGATCY 2 cut(s) 67, 97
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 2 cut(s) 67, 97
BstZ17I GTATAC 1 cut(s) 267
BsuRI GGCC 7 cut(s) 351, 605, 680, 1086, 1373, 1731, 1817
BtgI CCRYGG 2 cut(s) 681, 1752
BtsCI GGATG 3 cut(s) 849, 888, 1228
BtsIMutI CAGTG 6 cut(s) 123, 366, 530, 769, 1520, 1554
Cac8I GCNNGC 5 cut(s) 488, 783, 1549, 1574, 1656
CciI TCATGA 2 cut(s) 216, 723
CfoI GCGC 2 cut(s) 1654, 1660
Cfr13I GGNCC 2 cut(s) 169, 1816
Cfr42I CCGCGG 1 cut(s) 684
Cfr9I CCCGGG 1 cut(s) 1254
CseI GACGC 1 cut(s) 993
Csp6I GTAC 2 cut(s) 929, 1633
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviQI GTAC 2 cut(s) 929, 1633
DdeI CTNAG 3 cut(s) 580, 1125, 1401
DpnI GATC 9 cut(s) 69, 99, 221, 417, 635, 840, 1399, 1569, 1683
DpnII GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
EaeI YGGCCR 3 cut(s) 349, 603, 678
Eam1104I CTCTTC 3 cut(s) 750, 864, 870
EarI CTCTTC 3 cut(s) 750, 864, 870
Eco130I CCWWGG 1 cut(s) 1232
Eco147I AGGCCT 1 cut(s) 1731
Eco24I GRGCYC 1 cut(s) 1826
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 274, 324
Eco88I CYCGRG 2 cut(s) 71, 1254
Eco91I GGTNACC 1 cut(s) 1916
EcoO65I GGTNACC 1 cut(s) 1916
EcoRI GAATTC 1 cut(s) 1037
EcoRII CCWGG 2 cut(s) 346, 1818
EcoT14I CCWWGG 1 cut(s) 1232
EcoT22I ATGCAT 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 1826
ErhI CCWWGG 1 cut(s) 1232
FaqI GGGAC 3 cut(s) 98, 155, 991
FauI CCCGC 1 cut(s) 479
FauNDI CATATG 1 cut(s) 261
FblI GTMKAC 3 cut(s) 135, 266, 1517
FokI GGATG 3 cut(s) 836, 895, 1215
FriOI GRGCYC 1 cut(s) 1826
FspBI CTAG 4 cut(s) 1233, 1733, 1763, 1944
GlaI GCGC 2 cut(s) 1653, 1659
GsaI CCCAGC 1 cut(s) 693
HaeIII GGCC 7 cut(s) 351, 605, 680, 1086, 1373, 1731, 1817
HapII CCGG 6 cut(s) 154, 285, 352, 1087, 1255, 1562
HgaI GACGC 1 cut(s) 993
HhaI GCGC 2 cut(s) 1654, 1660
Hin6I GCGC 2 cut(s) 1652, 1658
HinP1I GCGC 2 cut(s) 1652, 1658
HincII GTYRAC 1 cut(s) 1518
HindII GTYRAC 1 cut(s) 1518
HindIII AAGCTT 1 cut(s) 1157
HinfI GANTC 6 cut(s) 458, 694, 758, 984, 1279, 1439
HpaII CCGG 6 cut(s) 154, 285, 352, 1087, 1255, 1562
HphI GGTGA 7 cut(s) 359, 551, 581, 1262, 1457, 1595, 1691
Hpy166II GTNNAC 5 cut(s) 52, 136, 267, 1270, 1518
Hpy188I TCNGA 8 cut(s) 304, 426, 993, 1193, 1221, 1251, 1327, 1402
Hpy8I GTNNAC 5 cut(s) 52, 136, 267, 1270, 1518
HpyAV CCTTC 4 cut(s) 184, 548, 792, 1931
HpyCH4IV ACGT 2 cut(s) 1806, 1912
HpyF10VI GCNNNNNNNGC 5 cut(s) 686, 740, 902, 1165, 1763
HpyF3I CTNAG 3 cut(s) 580, 1125, 1401
HpySE526I ACGT 2 cut(s) 1806, 1912
HspAI GCGC 2 cut(s) 1652, 1658
KspI CCGCGG 1 cut(s) 684
Kzo9I GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
LmnI GCTCC 2 cut(s) 1396, 1430
LweI GCATC 6 cut(s) 499, 873, 913, 949, 1237, 1576
MaeI CTAG 4 cut(s) 1233, 1733, 1763, 1944
MaeII ACGT 2 cut(s) 1806, 1912
MalI GATC 9 cut(s) 69, 99, 221, 417, 635, 840, 1399, 1569, 1683
MbiI CCGCTC 1 cut(s) 1864
MboI GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
MboII GAAGA 8 cut(s) 767, 829, 881, 887, 1088, 1462, 1676, 1850
MflI RGATCY 2 cut(s) 67, 97
MhlI GDGCHC 2 cut(s) 1672, 1826
MlsI TGGCCA 1 cut(s) 605
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 1 cut(s) 767
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 1 cut(s) 964
MroXI GAANNNNTTC 2 cut(s) 252, 1167
MscI TGGCCA 1 cut(s) 605
MseI TTAA 1 cut(s) 525
MslI CAYNNNNRTG 3 cut(s) 670, 854, 1628
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 3 cut(s) 683, 734, 1109
MspI CCGG 6 cut(s) 154, 285, 352, 1087, 1255, 1562
MspR9I CCNGG 5 cut(s) 155, 348, 1255, 1256, 1820
Mva1269I GAATGC 4 cut(s) 553, 640, 1304, 1757
MvaI CCWGG 2 cut(s) 348, 1820
MvnI CGCG 1 cut(s) 683
MwoI GCNNNNNNNGC 5 cut(s) 686, 740, 902, 1165, 1763
NciI CCSGG 3 cut(s) 155, 1255, 1256
NdeI CATATG 1 cut(s) 261
NdeII GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
NlaIV GGNNCC 2 cut(s) 99, 171
NmuCI GTSAC 2 cut(s) 400, 433
NsiI ATGCAT 1 cut(s) 964
NspI RCATGY 2 cut(s) 1345, 1576
OliI CACNNNNGTG 1 cut(s) 670
PaeI GCATGC 1 cut(s) 1576
PaeR7I CTCGAG 1 cut(s) 71
PagI TCATGA 2 cut(s) 216, 723
PasI CCCWGGG 1 cut(s) 1819
PceI AGGCCT 1 cut(s) 1731
PctI GAATGC 4 cut(s) 553, 640, 1304, 1757
PdmI GAANNNNTTC 2 cut(s) 252, 1167
PfeI GAWTC 5 cut(s) 458, 694, 984, 1279, 1439
Pfl23II CGTACG 1 cut(s) 928
Ple19I CGATCG 1 cut(s) 1570
PleI GAGTC 1 cut(s) 766
PpsI GAGTC 1 cut(s) 766
Ppu21I YACGTR 1 cut(s) 1913
PsiI TTATAA 1 cut(s) 1742
Psp6I CCWGG 2 cut(s) 346, 1818
PspEI GGTNACC 1 cut(s) 1916
PspFI CCCAGC 1 cut(s) 689
PspGI CCWGG 2 cut(s) 346, 1818
PspLI CGTACG 1 cut(s) 928
PspN4I GGNNCC 2 cut(s) 99, 171
PspPI GGNCC 2 cut(s) 169, 1816
PstI CTGCAG 2 cut(s) 64, 478
PsuI RGATCY 2 cut(s) 67, 97
PvuI CGATCG 1 cut(s) 1570
PvuII CAGCTG 2 cut(s) 734, 1109
RsaI GTAC 2 cut(s) 930, 1634
RsaNI GTAC 2 cut(s) 929, 1633
RseI CAYNNNNRTG 3 cut(s) 670, 854, 1628
SacII CCGCGG 1 cut(s) 684
SalI GTCGAC 1 cut(s) 1516
SaqAI TTAA 1 cut(s) 525
Sau3AI GATC 9 cut(s) 67, 97, 219, 415, 633, 838, 1397, 1567, 1681
Sau96I GGNCC 2 cut(s) 169, 1816
SchI GAGTC 1 cut(s) 767
ScrFI CCNGG 5 cut(s) 155, 348, 1255, 1256, 1820
SduI GDGCHC 2 cut(s) 1672, 1826
SfaNI GCATC 6 cut(s) 499, 873, 913, 949, 1237, 1576
SfcI CTRYAG 3 cut(s) 60, 78, 474
Sfr274I CTCGAG 1 cut(s) 71
Sfr303I CCGCGG 1 cut(s) 684
SgrBI CCGCGG 1 cut(s) 684
SinI GGWCC 1 cut(s) 169
SlaI CTCGAG 1 cut(s) 71
SmaI CCCGGG 1 cut(s) 1256
SmiMI CAYNNNNRTG 3 cut(s) 670, 854, 1628
SmlI CTYRAG 3 cut(s) 71, 1375, 1483
SmoI CTYRAG 3 cut(s) 71, 1375, 1483
SphI GCATGC 1 cut(s) 1576
SseBI AGGCCT 1 cut(s) 1731
SsiI CCGC 8 cut(s) 374, 464, 486, 551, 681, 683, 1014, 1862
SspI AATATT 1 cut(s) 1605
SspMI CTAG 4 cut(s) 1233, 1733, 1763, 1944
StuI AGGCCT 1 cut(s) 1731
StyD4I CCNGG 5 cut(s) 153, 346, 1253, 1254, 1818
StyI CCWWGG 1 cut(s) 1232
TaiI ACGT 2 cut(s) 1809, 1915
TaqI TCGA 6 cut(s) 11, 72, 618, 1308, 1351, 1517
TaqII GACCGA 1 cut(s) 181
TatI WGTACW 1 cut(s) 1632
TauI GCSGC 3 cut(s) 377, 683, 1017
TfiI GAWTC 5 cut(s) 458, 694, 984, 1279, 1439
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 6 cut(s) 130, 366, 537, 769, 1527, 1561
TseFI GTSAC 2 cut(s) 400, 433
Tsp45I GTSAC 2 cut(s) 400, 433
TspDTI ATGAA 6 cut(s) 54, 458, 1088, 1822, 1843, 1894
TspGWI ACGGA 1 cut(s) 1741
TspMI CCCGGG 1 cut(s) 1254
TspRI CASTG 6 cut(s) 130, 366, 537, 769, 1527, 1561
VpaK11BI GGWCC 1 cut(s) 169
XapI RAATTY 3 cut(s) 1037, 1245, 1492
XceI RCATGY 2 cut(s) 1345, 1576
XhoI CTCGAG 1 cut(s) 71
XmaI CCCGGG 1 cut(s) 1254
XmaJI CCTAGG 1 cut(s) 1232
XmiI GTMKAC 3 cut(s) 135, 266, 1517
XmnI GAANNNNTTC 2 cut(s) 252, 1167
XspI CTAG 4 cut(s) 1233, 1733, 1763, 1944
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.