MD12G1218700.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000494
Physical Location & Seq
Reverse (-)
29614212 .. 29616528
2317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1218700.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGGAAGAAGGTGCTGCTCATGATCATGAGCACGGCGTGGAAGATATCGAGATGGTGAACACTGCAGAGCAATGGAATTGGGAGGAGGAGGAGGAGGAGAATATTGACAAATGCAGGGATCGTGAAATACGCGAGCATGCAACAACTGTCAATGATCCGAACCGTACAAGACCAAAGCTCAGGTCAGAAATGTTCGAACAGAGAAGCACCCTTGGGAATTACTTTATTCACTTGGCTTCAAGTGTGGGCGATCTGGGGAGTGTCAAACTGATAACTGAGCTACATCCGTCGCAAGTCCTGAAACAAAACAATGAAGGAAACCTTCCACTTCATTTAGCAGCAACAGCAGGACGTCTATCTACGGTCAGCTTTCTGGTGGAAATTTCCCAAACTAACAATTATAATCTACTGGTTGAGAAGAATACGAGAGGGAACACCGCGTTGCACGTTGCCATGGAAAATCGCCAAAAAGAGGTGGCTAAGTTCTTGATTAATAATGACGAATCGAGGTTTTCTCCAAATATTTATGGCAAGACTCCCTTGTCCATGGCTGCTGAAGCTGGGAATGTTGAGCTCTTTGAACATATGATATTTGGCAGACCTAGTCGGTTGCCCCTTGACAATTTTCCAGAGGAACGATGGGAAGGGAACTCAGTTCTGCGCGCTGCCATTCTAGGAAGGAACAGAGAAATCATAAACATAGTCCTGCTATGCATTTCTTCATCTCTAGTCGGGGCAACGGATGAAGAGGGGATAACTCCTCTCACACTTGGGGCATATATAGATGACTTTGAAACTGTTAGCACCGTGCTAGCAACTGTCGGAGGTTCAGCTGCCGCAAACAAAGAGGACAAAAATGGCTTCACTCCCATACATTTGGCTTCCAGAAAGGGCCACATCACGATTGTTGAGTTGCTTCTCAAACATTGCCCACCTCCGAGGAACTTACTTGACAAAAACAACTGGAATATTCTTCACACGGCAGTGTGGCATCGAAGAGACAACATGGTCAAATATTTTCTTGGCAAGTTTGAGTTTCAAGAGTTGATTAATCAAAAGGATAAATTTGGAAACACTCCTCTGCATTTAGCCGTACGGCAAGGGTACCCAAAGACTGTCAGCATTTTGGCATCAAGCAAGAGAGTTAACTTCAGCGTTAGGAACAATGAAGGCCACTCAGCAATGGACATTGCACGAATGTCCTTGATGGGCGAAAAAACCTTGTTTCTTCGCCGGCCTCTAACGGTCATGGCCTTGACATTAGCAAGTGCTTCGCGTTCTCCAGAGAAGAGTATTTCTTCCAAAGTAAAGCCAACGGAAGCTAATTATTTCAACGTCACGGAGCAAAGGGGAGCATTTCTTTCTGTTGTCCCCGAGGACGCTAGTAGCCATATACAGACTGTCAATAATCTCCTACTGGTGTCAACACTCGTAGCCACTGTAACCTTTACTGCTGGCTTCACCATGCCAGGTGGATATATGAGCTCTGGAAACAACGAAGGCAAGTCAACTTTGGTTACAGATTGGCTATTCCAAACATTCTTGATATGCAACACCATAGCAATGTATAGCTCAATCACTGTTGCAATTGGTCTTTTGTGGGCGCAGACGGGTGATATGAGTTACGCTTGTTCAAAAGTTGGACCACCAATTTTAGGCTTAGCTCTTTCGATGATGCTTGTGGCGTTCATGGCTGGTGTTGCTTTGGTAACAAGTAACCTTACTTGGCTTGCCATATTCGTCGTCATCACGGGTTCAATCTTCCTCGTCATTGTATTACTGTTGCTTGTTTCATTGATCATCCCGATTTCTACCAAGCATCGCATTACATGTTTCGTTTTACGTTGTGTCTTCCATATGTTACTGAACATACCCAAGTATGACGATGTTGACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002376 GO:0002831 GO:0002833 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008219 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009628 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009816 GO:0009966 GO:0009987 GO:0010033 GO:0010646 GO:0012505 GO:0014070 GO:0016020 GO:0016021 GO:0016567 GO:0019538 GO:0023051 GO:0030176 GO:0031224 GO:0031226 GO:0031227 GO:0031347 GO:0031349 GO:0031984 GO:0032101 GO:0032103 GO:0032446 GO:0032870 GO:0035690 GO:0036211 GO:0042175 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0045087 GO:0046677 GO:0048518 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0098827 GO:1900150 GO:1900424 GO:1900426 GO:1901564 GO:1901700 GO:1901701 GO:1902288 GO:1902290 GO:2000031 GO:2001023 GO:2001038
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 PGG

Protein Analysis

633

Amino Acids

70.2

Weight (kDa)

6.12

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 76 - 166 7e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 140 - 195 1.1e-08 Ankyrin repeats (3 copies)
Ank_5 PF13857 275 - 308 1e-05 Ankyrin repeats (many copies)
Ank_2 PF12796 276 - 342 3.5e-12 Ankyrin repeats (3 copies)
Ank PF00023 286 - 311 6.5e-06 Ankyrin repeat
Ank_5 PF13857 306 - 364 3.4e-09 Ankyrin repeats (many copies)
PGG PF13962 466 - 569 3.9e-26 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000494)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G03670 AT4G03440 AT4G03440 AT4G03440 AT4G03450 AT4G03450 AT4G03460 AT4G03460 AT4G03470 AT4G03480 AT4G03480 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03490 AT4G03500 AT4G03500 AT4G03500 AT4G03505 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G05040 AT4G14390 AT4G14390 AT4G14390 AT4G14390 AT4G14400 AT4G14400 AT4G14400
fragaria_vesca FvH4_3g05910 FvH4_3g23320
malus_domestica MD03G1094000.v1.1 MD03G1094200.v1.1 MD03G1094600.v1.1 MD12G1218700.v1.1
prunus_persica Prupe.3G135100_v2.0.a1 Prupe.3G135200_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G135700_v2.0.a1 Prupe.3G142400_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G153800_v2.0.a1 Prupe.3G154000_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154200_v2.0.a1 Prupe.3G154600_v2.0.a1 Prupe.3G154700_v2.0.a1 Prupe.3G155200_v2.0.a1 Prupe.3G155300_v2.0.a1 Prupe.8G110300_v2.0.a1
pyrus_communis pycom03g07280 pycom03g07580 pycom03g07660 pycom03g07700 pycom03g07710
rosa_chinensis RchiOBHm_Chr3g0461161 RchiOBHm_Chr5g0041111
rosa_laevigata RLG00000023472 RLG00000023475 RLG00000034025
rosa_multiflora Rmu_co8126040.1_g000001 Rmu_co8268563.1_g000001 Rmu_sc0000964.1_g000014 Rmu_sc0028923.1_g000001 Rmu_sc0042194.1_g000001
rosa_roxburghii Rroxscaffold_1G00039610 Rroxscaffold_6G00401360
rosa_rugosa Rorug03G0048600 Rorug03G0048700 Rorug05G0190000
rosa_samantha Rh3AG105900 Rh3BG110200 Rh3BG268400 Rh3CG111800 Rh3DG111900 Rh5AG275800 Rh5BG280500 Rh5CG312600 Rh5DG288800
rosa_wichuraiana Rw3G009080 Rw5G025920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AasI GACNNNNNNGTC 2 cut(s) 541, 1007
AatII GACGTC 1 cut(s) 355
Acc65I GGTACC 1 cut(s) 1104
AccB1I GGYRCC 1 cut(s) 1104
AccII CGCG 4 cut(s) 132, 440, 663, 1276
AciI CCGC 2 cut(s) 438, 837
AclWI GGATC 2 cut(s) 126, 149
AcsI RAATTY 2 cut(s) 381, 1064
AcuI CTGAAG 2 cut(s) 576, 1135
AcyI GRCGYC 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 37
AfaI GTAC 3 cut(s) 166, 1095, 1106
AfiI CCNNNNNNNGG 2 cut(s) 472, 1655
AflIII ACRYGT 1 cut(s) 1829
AgsI TTSAA 7 cut(s) 240, 581, 794, 1040, 1333, 1635, 1758
AjnI CCWGG 1 cut(s) 1468
AleI CACNNNNGTG 1 cut(s) 983
Alw21I GWGCWC 3 cut(s) 33, 576, 1487
Alw26I GTCTC 1 cut(s) 993
AlwI GGATC 2 cut(s) 126, 149
Ama87I CYCGRG 1 cut(s) 1373
AoxI GGCC 4 cut(s) 892, 1171, 1235, 1251
ApeKI GCWGC 5 cut(s) 14, 338, 551, 665, 833
ApoI RAATTY 2 cut(s) 381, 1064
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 2 cut(s) 492, 1050
Asp718I GGTACC 1 cut(s) 1104
AspLEI GCGC 3 cut(s) 663, 665, 1606
AspS9I GGNCC 2 cut(s) 892, 1643
AsuHPI GGTGA 3 cut(s) 67, 1453, 1625
AsuII TTCGAA 1 cut(s) 195
AsuNHI GCTAGC 1 cut(s) 811
AvaI CYCGRG 1 cut(s) 1373
AvaII GGWCC 1 cut(s) 1643
BanI GGYRCC 1 cut(s) 1104
BanII GRGCYC 2 cut(s) 576, 1487
BbsI GAAGAC 1 cut(s) 1843
Bbv12I GWGCWC 3 cut(s) 33, 576, 1487
BbvI GCAGC 4 cut(s) 350, 538, 652, 820
BccI CCATC 3 cut(s) 46, 633, 1201
BceAI ACGGC 4 cut(s) 49, 996, 1076, 1112
BcgI CGANNNNNNTGC 2 cut(s) 1254, 1288
BciT130I CCWGG 1 cut(s) 1470
BclI TGATCA 2 cut(s) 22, 1797
BcoDI GTCTC 1 cut(s) 993
BfaI CTAG 5 cut(s) 603, 674, 728, 812, 1383
BfmI CTRYAG 1 cut(s) 63
BisI GCNGC 6 cut(s) 15, 339, 552, 666, 834, 837
BlpI GCTNAGC 1 cut(s) 1660
BlsI GCNGC 6 cut(s) 16, 340, 553, 667, 835, 838
Bme1390I CCNGG 1 cut(s) 1470
Bme18I GGWCC 1 cut(s) 1643
BmeT110I CYCGRG 1 cut(s) 1373
BmgT120I GGNCC 2 cut(s) 892, 1643
BmiI GGNNCC 1 cut(s) 1106
BmrFI CCNGG 1 cut(s) 1470
BmsI GCATC 4 cut(s) 1000, 1139, 1665, 1828
BmtI GCTAGC 1 cut(s) 815
BpiI GAAGAC 1 cut(s) 1843
BplI GAGNNNNNCTC 2 cut(s) 499, 531
BpmI CTGGAG 1 cut(s) 1266
Bpu10I CCTNAGC 1 cut(s) 179
Bpu1102I GCTNAGC 1 cut(s) 1660
Bpu14I TTCGAA 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 352
BsaJI CCNNGG 5 cut(s) 211, 453, 546, 938, 1374
Bsc4I CCNNNNNNNGG 2 cut(s) 472, 1655
Bse118I RCCGGY 1 cut(s) 1233
Bse1I ACTGG 3 cut(s) 414, 968, 1422
Bse3DI GCAATG 5 cut(s) 77, 925, 1188, 1188, 1569
BseBI CCWGG 1 cut(s) 1470
BseDI CCNNGG 5 cut(s) 211, 453, 546, 938, 1374
BseGI GGATG 3 cut(s) 283, 748, 1800
BseLI CCNNNNNNNGG 2 cut(s) 472, 1655
BseMI GCAATG 5 cut(s) 77, 925, 1188, 1188, 1569
BseMII CTCAG 4 cut(s) 193, 267, 666, 1191
BseNI ACTGG 3 cut(s) 414, 968, 1422
BsePI GCGCGC 1 cut(s) 661
BseRI GAGGAG 7 cut(s) 98, 101, 104, 107, 110, 750, 1068
BseXI GCAGC 4 cut(s) 350, 538, 652, 820
BseYI CCCAGC 1 cut(s) 560
Bsh1236I CGCG 4 cut(s) 132, 440, 663, 1276
BshFI GGCC 4 cut(s) 894, 1173, 1237, 1253
BshNI GGYRCC 1 cut(s) 1104
BsiHKAI GWGCWC 3 cut(s) 33, 576, 1487
BsiHKCI CYCGRG 1 cut(s) 1373
BsiSI CCGG 1 cut(s) 1234
BsiWI CGTACG 1 cut(s) 1093
BslFI GGGAC 1 cut(s) 1355
BslI CCNNNNNNNGG 2 cut(s) 472, 1655
BsmAI GTCTC 1 cut(s) 993
BsmFI GGGAC 1 cut(s) 1355
BsnI GGCC 4 cut(s) 894, 1173, 1237, 1253
BsoBI CYCGRG 1 cut(s) 1373
Bsp119I TTCGAA 1 cut(s) 195
Bsp1286I GDGCHC 3 cut(s) 33, 576, 1487
Bsp143I GATC 5 cut(s) 22, 118, 154, 250, 1797
Bsp1720I GCTNAGC 1 cut(s) 1660
Bsp19I CCATGG 2 cut(s) 453, 546
BspACI CCGC 2 cut(s) 438, 837
BspANI GGCC 4 cut(s) 894, 1173, 1237, 1253
BspCNI CTCAG 4 cut(s) 192, 268, 665, 1190
BspFNI CGCG 4 cut(s) 132, 440, 663, 1276
BspHI TCATGA 2 cut(s) 19, 25
BspLI GGNNCC 1 cut(s) 1106
BspMAI CTGCAG 1 cut(s) 67
BspOI GCTAGC 1 cut(s) 815
BspPI GGATC 2 cut(s) 126, 149
BspT104I TTCGAA 1 cut(s) 195
BspT107I GGYRCC 1 cut(s) 1104
BsrDI GCAATG 5 cut(s) 77, 925, 1188, 1188, 1569
BsrFI RCCGGY 1 cut(s) 1233
BsrI ACTGG 3 cut(s) 414, 968, 1422
BssAI RCCGGY 1 cut(s) 1233
BssECI CCNNGG 5 cut(s) 211, 453, 546, 938, 1374
BssHII GCGCGC 1 cut(s) 661
BssMI GATC 5 cut(s) 22, 118, 154, 250, 1797
BssNI GRCGYC 1 cut(s) 352
BssT1I CCWWGG 3 cut(s) 211, 453, 546
Bst2UI CCWGG 1 cut(s) 1470
Bst6I CTCTTC 3 cut(s) 741, 991, 1283
BstACI GRCGYC 1 cut(s) 352
BstBI TTCGAA 1 cut(s) 195
BstC8I GCNNGC 7 cut(s) 134, 138, 663, 813, 1235, 1456, 1731
BstDEI CTNAG 6 cut(s) 179, 276, 480, 652, 1177, 1660
BstDSI CCRYGG 2 cut(s) 453, 546
BstF5I GGATG 3 cut(s) 283, 748, 1800
BstFNI CGCG 4 cut(s) 132, 440, 663, 1276
BstHHI GCGC 3 cut(s) 663, 665, 1606
BstKTI GATC 5 cut(s) 25, 121, 157, 253, 1800
BstMAI GTCTC 1 cut(s) 993
BstMBI GATC 5 cut(s) 22, 118, 154, 250, 1797
BstMWI GCNNNNNNNGC 4 cut(s) 344, 557, 1691, 1700
BstNI CCWGG 1 cut(s) 1470
BstNSI RCATGY 2 cut(s) 140, 1833
BstSCI CCNGG 1 cut(s) 1468
BstSFI CTRYAG 1 cut(s) 63
BstUI CGCG 4 cut(s) 132, 440, 663, 1276
BstV1I GCAGC 4 cut(s) 350, 538, 652, 820
BstV2I GAAGAC 1 cut(s) 1843
BstXI CCANNNNNNTGG 1 cut(s) 877
BsuRI GGCC 4 cut(s) 894, 1173, 1237, 1253
BtgI CCRYGG 2 cut(s) 453, 546
BtgZI GCGATG 1 cut(s) 1805
BtsCI GGATG 3 cut(s) 283, 748, 1800
BtsI GCAGTG 2 cut(s) 60, 990
BtsIMutI CAGTG 4 cut(s) 60, 990, 1437, 1578
Cac8I GCNNGC 7 cut(s) 134, 138, 663, 813, 1235, 1456, 1731
CciI TCATGA 2 cut(s) 19, 25
CfoI GCGC 3 cut(s) 663, 665, 1606
Cfr10I RCCGGY 1 cut(s) 1233
Cfr13I GGNCC 2 cut(s) 892, 1643
CseI GACGC 1 cut(s) 1388
Csp6I GTAC 3 cut(s) 165, 1094, 1105
CviQI GTAC 3 cut(s) 165, 1094, 1105
DdeI CTNAG 6 cut(s) 179, 276, 480, 652, 1177, 1660
DpnI GATC 5 cut(s) 24, 120, 156, 252, 1799
DpnII GATC 5 cut(s) 22, 118, 154, 250, 1797
DraIII CACNNNGTG 1 cut(s) 37
DrdI GACNNNNNNGTC 2 cut(s) 541, 1007
DseDI GACNNNNNNGTC 2 cut(s) 541, 1007
Eam1104I CTCTTC 3 cut(s) 741, 991, 1283
EarI CTCTTC 3 cut(s) 741, 991, 1283
Ecl136II GAGCTC 2 cut(s) 574, 1485
Eco130I CCWWGG 3 cut(s) 211, 453, 546
Eco24I GRGCYC 2 cut(s) 576, 1487
Eco32I GATATC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 1643
Eco53kI GAGCTC 2 cut(s) 574, 1485
Eco57I CTGAAG 2 cut(s) 576, 1135
Eco88I CYCGRG 1 cut(s) 1373
EcoICRI GAGCTC 2 cut(s) 574, 1485
EcoRII CCWGG 1 cut(s) 1468
EcoRV GATATC 1 cut(s) 46
EcoT14I CCWWGG 3 cut(s) 211, 453, 546
EcoT22I ATGCAT 1 cut(s) 716
EcoT38I GRGCYC 2 cut(s) 576, 1487
ErhI CCWWGG 3 cut(s) 211, 453, 546
FalI AAGNNNNNCTT 4 cut(s) 306, 338, 524, 556
FaqI GGGAC 1 cut(s) 1355
FauNDI CATATG 2 cut(s) 585, 1857
FbaI TGATCA 2 cut(s) 22, 1797
Fnu4HI GCNGC 6 cut(s) 15, 339, 552, 666, 834, 837
FokI GGATG 3 cut(s) 270, 755, 1787
FriOI GRGCYC 2 cut(s) 576, 1487
Fsp4HI GCNGC 6 cut(s) 15, 339, 552, 666, 834, 837
FspBI CTAG 5 cut(s) 603, 674, 728, 812, 1383
GlaI GCGC 3 cut(s) 662, 664, 1605
GluI GCNGC 6 cut(s) 15, 339, 552, 666, 834, 837
GsaI CCCAGC 1 cut(s) 564
GsuI CTGGAG 1 cut(s) 1266
HaeIII GGCC 4 cut(s) 894, 1173, 1237, 1253
HapII CCGG 1 cut(s) 1234
HgaI GACGC 1 cut(s) 1388
HhaI GCGC 3 cut(s) 663, 665, 1606
Hin1I GRCGYC 1 cut(s) 352
Hin6I GCGC 3 cut(s) 661, 663, 1604
HinP1I GCGC 3 cut(s) 661, 663, 1604
HincII GTYRAC 4 cut(s) 1147, 1425, 1509, 1891
HindII GTYRAC 4 cut(s) 1147, 1425, 1509, 1891
HinfI GANTC 2 cut(s) 503, 535
HpaI GTTAAC 1 cut(s) 1147
HpaII CCGG 1 cut(s) 1234
HphI GGTGA 3 cut(s) 67, 1453, 1625
Hpy166II GTNNAC 5 cut(s) 58, 1147, 1425, 1509, 1891
Hpy188I TCNGA 4 cut(s) 159, 187, 824, 939
Hpy8I GTNNAC 5 cut(s) 58, 1147, 1425, 1509, 1891
Hpy99I CGWCG 2 cut(s) 292, 1745
HpyAV CCTTC 6 cut(s) 308, 332, 638, 672, 1163, 1493
HpyCH4IV ACGT 4 cut(s) 352, 447, 1335, 1843
HpyCH4V TGCA 9 cut(s) 65, 114, 140, 445, 714, 1084, 1193, 1551, 1586
HpyF10VI GCNNNNNNNGC 4 cut(s) 344, 557, 1691, 1700
HpyF3I CTNAG 6 cut(s) 179, 276, 480, 652, 1177, 1660
HpySE526I ACGT 4 cut(s) 352, 447, 1335, 1843
Hsp92I GRCGYC 1 cut(s) 352
HspAI GCGC 3 cut(s) 661, 663, 1604
KpnI GGTACC 1 cut(s) 1108
KroI GCCGGC 1 cut(s) 1233
KroNI GCCGGC 1 cut(s) 1235
Ksp22I TGATCA 2 cut(s) 22, 1797
KspAI GTTAAC 1 cut(s) 1147
Kzo9I GATC 5 cut(s) 22, 118, 154, 250, 1797
LmnI GCTCC 2 cut(s) 1342, 1352
Lsp1109I GCAGC 4 cut(s) 350, 538, 652, 820
LweI GCATC 4 cut(s) 1000, 1139, 1665, 1828
MaeI CTAG 5 cut(s) 603, 674, 728, 812, 1383
MaeII ACGT 4 cut(s) 352, 447, 1335, 1843
MaeIII GTNAC 7 cut(s) 1336, 1441, 1516, 1622, 1708, 1715, 1860
MalI GATC 5 cut(s) 24, 120, 156, 252, 1799
MboI GATC 5 cut(s) 22, 118, 154, 250, 1797
MfeI CAATTG 1 cut(s) 1587
MhlI GDGCHC 3 cut(s) 33, 576, 1487
MluCI AATT 9 cut(s) 76, 217, 381, 397, 622, 1064, 1324, 1587, 1650
MlyI GAGTC 1 cut(s) 529
MmeI TCCRAC 2 cut(s) 802, 1621
Mph1103I ATGCAT 1 cut(s) 716
MroNI GCCGGC 1 cut(s) 1233
MseI TTAA 3 cut(s) 492, 1050, 1146
MslI CAYNNNNRTG 3 cut(s) 24, 983, 1562
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 1 cut(s) 1234
MspR9I CCNGG 1 cut(s) 1470
MunI CAATTG 1 cut(s) 1587
MvaI CCWGG 1 cut(s) 1470
MvnI CGCG 4 cut(s) 132, 440, 663, 1276
MwoI GCNNNNNNNGC 4 cut(s) 344, 557, 1691, 1700
NaeI GCCGGC 1 cut(s) 1235
NcoI CCATGG 2 cut(s) 453, 546
NdeI CATATG 2 cut(s) 585, 1857
NdeII GATC 5 cut(s) 22, 118, 154, 250, 1797
NgoMIV GCCGGC 1 cut(s) 1233
NheI GCTAGC 1 cut(s) 811
NlaIV GGNNCC 1 cut(s) 1106
NmuCI GTSAC 1 cut(s) 1336
NsiI ATGCAT 1 cut(s) 716
NspI RCATGY 2 cut(s) 140, 1833
NspV TTCGAA 1 cut(s) 195
OliI CACNNNNGTG 1 cut(s) 983
PaeI GCATGC 1 cut(s) 140
PagI TCATGA 2 cut(s) 19, 25
PauI GCGCGC 1 cut(s) 661
PciI ACATGT 1 cut(s) 1829
PcsI WCGNNNNNNNCGW 1 cut(s) 1890
PdiI GCCGGC 1 cut(s) 1235
PfeI GAWTC 1 cut(s) 503
Pfl23II CGTACG 1 cut(s) 1093
PflFI GACNNNGTC 1 cut(s) 603
PkrI GCNGC 6 cut(s) 16, 340, 553, 667, 835, 838
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
PscI ACATGT 1 cut(s) 1829
PshBI ATTAAT 2 cut(s) 492, 1050
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 576, 1487
Psp6I CCWGG 1 cut(s) 1468
PspFI CCCAGC 1 cut(s) 560
PspGI CCWGG 1 cut(s) 1468
PspLI CGTACG 1 cut(s) 1093
PspN4I GGNNCC 1 cut(s) 1106
PspPI GGNCC 2 cut(s) 892, 1643
PstI CTGCAG 1 cut(s) 67
PsyI GACNNNGTC 1 cut(s) 603
PteI GCGCGC 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 3 cut(s) 166, 1095, 1106
RsaNI GTAC 3 cut(s) 165, 1094, 1105
RseI CAYNNNNRTG 3 cut(s) 24, 983, 1562
SacI GAGCTC 2 cut(s) 576, 1487
SaqAI TTAA 3 cut(s) 492, 1050, 1146
SatI GCNGC 6 cut(s) 15, 339, 552, 666, 834, 837
Sau3AI GATC 5 cut(s) 22, 118, 154, 250, 1797
Sau96I GGNCC 2 cut(s) 892, 1643
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 1 cut(s) 1470
SduI GDGCHC 3 cut(s) 33, 576, 1487
SfaNI GCATC 4 cut(s) 1000, 1139, 1665, 1828
SfcI CTRYAG 1 cut(s) 63
SfuI TTCGAA 1 cut(s) 195
SinI GGWCC 1 cut(s) 1643
SmiMI CAYNNNNRTG 3 cut(s) 24, 983, 1562
SphI GCATGC 1 cut(s) 140
Sse9I AATT 9 cut(s) 76, 217, 381, 397, 622, 1064, 1324, 1587, 1650
SsiI CCGC 2 cut(s) 438, 837
SspI AATATT 4 cut(s) 103, 523, 970, 1016
SspMI CTAG 5 cut(s) 603, 674, 728, 812, 1383
SstI GAGCTC 2 cut(s) 576, 1487
StyD4I CCNGG 1 cut(s) 1468
StyI CCWWGG 3 cut(s) 211, 453, 546
TaiI ACGT 4 cut(s) 355, 450, 1338, 1846
TaqI TCGA 5 cut(s) 48, 195, 506, 994, 1670
TasI AATT 9 cut(s) 76, 217, 381, 397, 622, 1064, 1324, 1587, 1650
TauI GCSGC 1 cut(s) 839
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 3 cut(s) 492, 1050, 1146
Tru9I TTAA 3 cut(s) 492, 1050, 1146
TscAI CASTG 4 cut(s) 67, 990, 1444, 1585
TseFI GTSAC 1 cut(s) 1336
TseI GCWGC 5 cut(s) 14, 338, 551, 665, 833
Tsp45I GTSAC 1 cut(s) 1336
TspDTI ATGAA 7 cut(s) 320, 327, 711, 759, 1182, 1678, 1782
TspGWI ACGGA 4 cut(s) 276, 755, 1331, 1355
TspRI CASTG 4 cut(s) 67, 990, 1444, 1585
Tth111I GACNNNGTC 1 cut(s) 603
VpaK11BI GGWCC 1 cut(s) 1643
VspI ATTAAT 2 cut(s) 492, 1050
XapI RAATTY 2 cut(s) 381, 1064
XceI RCATGY 2 cut(s) 140, 1833
XcmI CCANNNNNNNNNTGG 1 cut(s) 636
XspI CTAG 5 cut(s) 603, 674, 728, 812, 1383
ZraI GACGTC 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.