MD05G1340100.v1.1

transmembrane receptor protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000699
Physical Location & Seq
Reverse (-)
46080485 .. 46083218
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1340100.v1.1.491

Sequence Viewer

Length: 1119 bp
ATGAGGCAATGGAATATGTTCTTGGGTGTAGGAGTGCTCATGAAGCATGGACCAATCTTGTTGATAGGAAGAAGGAAACACCTCTCTACAATTATCATTGCTGTCGGGTGTGTTGGCATTGCTCTTCTAGTTGTCTCTCTAGTTCTTGTAGCATCGGGCTACTGCTTTCCAGGCAGACGAGAAAGAAAGAAGAATGATGAGGCTCTACAACATGAAAATGGCAAACAGCAAGATATGAGGACGGCTGAGTCCTTGCAATTTGATTTGGGAACTCTCGAAACTGCCACAAATAAGTTCTCCGAAGATAACAAGTTAGGCGAAGGTGGATTCGGTGCAGTTTTCAAGGGAACGCTTATTAATGGACAAGAAATAGCAGTAAAGAGGCTGTCAAAAAGCTCCGGACAAGGTGTACAAGAATTTAAGAATGAGGTAGTATTGGTAGCCAAGCTTCAACACAGAAATCTTGTCAGGCTTTTAGGATTTTGCTTGGAAGGAGAAGAAACCTTACTTGTTTATGAAAATGTGGCCAACAAAAGCCTTGATTATTTTCTATTTGAACATAAAAAACGAGAACAACTAGATTGGTTGAGCCGCACCATGATAATTGAAGGAATTGCTCGAGGAATTTTGTATCTCCATGAAGATTCGAGGCTTAGAGTTATACATCGTGATTTGAAAGCTAGCAACATTTTATTAGACAGCAACATGAACCCGAAAATATCAGATTTTGGGATGGCTAGAATGTTTGGAGTTGATGATCAAACTGAAGGAAATACCAAAAGAATTGTTGGCACTTATGGTTACATGGCTCCAGAATATGCTATGGAAGGGTTGTATTCAGTAAAATCGGATGTCTTCAGCTTCGGAGTACTTCTGCTTGAGATCATAATGGGGAGAAGGAACTTTTTAGGCTTTCATCGCACTAATTGTGGACCTACTCTTATAGGTTATGCTTGGCAATTATGGAATGAAACGAAAGGTTTGGAGTTGATGGATCCCTTGTTAAAAGATTCATGCAGTCCAAATGAATTTTTGAGATATGTTGATCACCATGATCAAGTGCGTGCTCAAGATTGCTCAGCCAATGGTTTCACGATTTCTATCGATGTTCCTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr Stress-antifung

Protein Analysis

373

Amino Acids

41.98

Weight (kDa)

7.66

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 100 - 304 8.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 102 - 303 1.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61750
fragaria_vesca FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754
malus_domestica MD02G1101300.v1.1 MD05G1031700.v1.1 MD05G1031800.v1.1 MD05G1032400.v1.1 MD05G1032600.v1.1 MD05G1337700.v1.1 MD05G1337900.v1.1 MD05G1340100.v1.1 MD05G1340400.v1.1 MD16G1096700.v1.1
prunus_persica Prupe.4G027600_v2.0.a1 Prupe.8G039200_v2.0.a1
pyrus_communis pycom05g02310 pycom05g02330 pycom05g02340 pycom05g30920
rosa_chinensis RchiOBHm_Chr2g0121501 RchiOBHm_Chr2g0122141 RchiOBHm_Chr5g0004191 RchiOBHm_Chr6g0265241 RchiOBHm_Chr7g0215811 RchiOBHm_Chr7g0215821 RchiOBHm_Chr7g0219681
rosa_laevigata RLG00000002617 RLG00000018618 RLG00000018623 RLG00000031211
rosa_multiflora Rmu_sc0000079.1_g000015 Rmu_sc0000315.1_g000072 Rmu_sc0000315.1_g000093 Rmu_sc0000547.1_g000005 Rmu_sc0000805.1_g000048 Rmu_sc0001851.1_g000024 Rmu_sc0004379.1_g000017 Rmu_sc0004574.1_g000057 Rmu_sc0004689.1_g000004 Rmu_sc0004964.1_g000019 Rmu_sc0007799.1_g000001 Rmu_sc0007868.1_g000012 Rmu_ssc0000359.1_g000013
rosa_roxburghii Rroxscaffold_175G00432180 Rroxscaffold_1G00071500 Rroxscaffold_1G00071530 Rroxscaffold_1G00071780 Rroxscaffold_2G00122400 Rroxscaffold_2G00122420
rosa_rugosa Rorug06G0063800
rosa_samantha Rh2AG290300 Rh2BG301100 Rh2CG279800 Rh5CG038400 Rh5CG040800 Rh5DG036900 Rh6CG182400 Rh6DG173600 Rh7CG314200 Rh7DG297100
rosa_wichuraiana Rw0G021200 Rw0G021210 Rw2G023270 Rw2G023510 Rw5G003650 Rw5G030050 Rw6G011890 Rw7G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 398
AciI CCGC 1 cut(s) 592
AclWI GGATC 2 cut(s) 989, 1002
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 3 cut(s) 416, 624, 1028
AcuI CTGAAG 2 cut(s) 786, 841
AfaI GTAC 2 cut(s) 411, 870
AgsI TTSAA 5 cut(s) 343, 452, 557, 608, 676
AjnI CCWGG 1 cut(s) 169
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 4 cut(s) 396, 448, 680, 861
AluI AGCT 4 cut(s) 396, 448, 680, 861
Alw21I GWGCWC 2 cut(s) 39, 1069
Alw26I GTCTC 1 cut(s) 139
AlwI GGATC 2 cut(s) 989, 1002
Ama87I CYCGRG 1 cut(s) 618
Aor13HI TCCGGA 1 cut(s) 398
AoxI GGCC 1 cut(s) 525
ApoI RAATTY 3 cut(s) 416, 624, 1028
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 50, 932
AsuHPI GGTGA 1 cut(s) 1040
AsuNHI GCTAGC 1 cut(s) 680
AvaI CYCGRG 1 cut(s) 618
AvaII GGWCC 2 cut(s) 50, 932
BalI TGGCCA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 994
BarI GAAGNNNNNNTAC 4 cut(s) 432, 464, 489, 521
BbsI GAAGAC 1 cut(s) 847
Bbv12I GWGCWC 2 cut(s) 39, 1069
BccI CCATC 2 cut(s) 727, 985
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 1 cut(s) 171
BclI TGATCA 3 cut(s) 757, 1045, 1054
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 5 cut(s) 128, 140, 578, 681, 738
BisI GCNGC 1 cut(s) 592
BlpI GCTNAGC 1 cut(s) 1078
BlsI GCNGC 1 cut(s) 593
BmcAI AGTACT 1 cut(s) 870
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 2 cut(s) 50, 932
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 2 cut(s) 50, 932
BmiI GGNNCC 2 cut(s) 810, 996
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 161
BmtI GCTAGC 1 cut(s) 684
BpiI GAAGAC 1 cut(s) 847
BpmI CTGGAG 1 cut(s) 795
Bpu1102I GCTNAGC 1 cut(s) 1078
BpuEI CTTGAG 2 cut(s) 899, 1053
Bsa29I ATCGAT 1 cut(s) 1104
BsaBI GATNNNNATC 1 cut(s) 1100
BsaWI WCCGGW 1 cut(s) 398
Bse3DI GCAATG 3 cut(s) 14, 96, 117
Bse8I GATNNNNATC 1 cut(s) 1100
BseAI TCCGGA 1 cut(s) 398
BseBI CCWGG 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 1104
BseGI GGATG 2 cut(s) 738, 856
BseJI GATNNNNATC 1 cut(s) 1100
BseMI GCAATG 3 cut(s) 14, 96, 117
BseMII CTCAG 2 cut(s) 237, 1092
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 1 cut(s) 527
BshVI ATCGAT 1 cut(s) 1104
BsiHKAI GWGCWC 2 cut(s) 39, 1069
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 139
BsnI GGCC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 618
Bsp1286I GDGCHC 2 cut(s) 39, 1069
Bsp13I TCCGGA 1 cut(s) 398
Bsp1407I TGTACA 1 cut(s) 409
Bsp143I GATC 5 cut(s) 757, 882, 994, 1045, 1054
Bsp1720I GCTNAGC 1 cut(s) 1078
BspACI CCGC 1 cut(s) 592
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 2 cut(s) 238, 1091
BspDI ATCGAT 1 cut(s) 1104
BspEI TCCGGA 1 cut(s) 398
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 2 cut(s) 810, 996
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 2 cut(s) 989, 1002
BspQI GCTCTTC 1 cut(s) 129
BsrDI GCAATG 3 cut(s) 14, 96, 117
BsrGI TGTACA 1 cut(s) 409
BssMI GATC 5 cut(s) 757, 882, 994, 1045, 1054
Bst2UI CCWGG 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 129
BstAUI TGTACA 1 cut(s) 409
BstC8I GCNNGC 2 cut(s) 682, 1065
BstDEI CTNAG 3 cut(s) 246, 653, 1078
BstF5I GGATG 2 cut(s) 738, 856
BstKTI GATC 5 cut(s) 760, 885, 997, 1048, 1057
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 5 cut(s) 757, 882, 994, 1045, 1054
BstMWI GCNNNNNNNGC 3 cut(s) 43, 171, 918
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 847
BstX2I RGATCY 1 cut(s) 994
BstYI RGATCY 1 cut(s) 994
Bsu15I ATCGAT 1 cut(s) 1104
BsuRI GGCC 1 cut(s) 527
BsuTUI ATCGAT 1 cut(s) 1104
BtgZI GCGATG 1 cut(s) 902
BtsCI GGATG 2 cut(s) 738, 856
Cac8I GCNNGC 2 cut(s) 682, 1065
CciI TCATGA 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 50, 932
ClaI ATCGAT 1 cut(s) 1104
Csp6I GTAC 2 cut(s) 410, 869
CviAII CATG 9 cut(s) 40, 47, 212, 598, 638, 706, 805, 1014, 1052
CviQI GTAC 2 cut(s) 410, 869
DdeI CTNAG 3 cut(s) 246, 653, 1078
DpnI GATC 5 cut(s) 759, 884, 996, 1047, 1056
DpnII GATC 5 cut(s) 757, 882, 994, 1045, 1054
DrdI GACNNNNNNGTC 1 cut(s) 247
DseDI GACNNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 50, 932
Eco57I CTGAAG 2 cut(s) 786, 841
Eco88I CYCGRG 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 169
FaeI CATG 9 cut(s) 43, 50, 215, 601, 641, 709, 808, 1017, 1055
FatI CATG 9 cut(s) 39, 46, 211, 597, 637, 705, 804, 1013, 1051
FbaI TGATCA 3 cut(s) 757, 1045, 1054
Fnu4HI GCNGC 1 cut(s) 592
FokI GGATG 2 cut(s) 745, 863
Fsp4HI GCNGC 1 cut(s) 592
FspBI CTAG 5 cut(s) 128, 140, 578, 681, 738
GluI GCNGC 1 cut(s) 592
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 1 cut(s) 527
HapII CCGG 1 cut(s) 399
Hin1II CATG 9 cut(s) 43, 50, 215, 601, 641, 709, 808, 1017, 1055
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 4 cut(s) 248, 327, 644, 1010
HpaII CCGG 1 cut(s) 399
HphI GGTGA 1 cut(s) 1040
Hpy166II GTNNAC 2 cut(s) 410, 932
Hpy188I TCNGA 4 cut(s) 301, 724, 850, 866
Hpy188III TCNNGA 7 cut(s) 40, 275, 399, 668, 812, 1070, 1093
Hpy8I GTNNAC 2 cut(s) 410, 932
HpyAV CCTTC 7 cut(s) 66, 314, 485, 602, 761, 821, 891
HpyCH4V TGCA 3 cut(s) 256, 335, 1017
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 171, 918
HpyF3I CTNAG 3 cut(s) 246, 653, 1078
Hsp92II CATG 9 cut(s) 43, 50, 215, 601, 641, 709, 808, 1017, 1055
Kpn2I TCCGGA 1 cut(s) 398
Ksp22I TGATCA 3 cut(s) 757, 1045, 1054
Kzo9I GATC 5 cut(s) 757, 882, 994, 1045, 1054
LguI GCTCTTC 1 cut(s) 129
LmnI GCTCC 2 cut(s) 401, 814
LpnPI CCDG 5 cut(s) 156, 183, 412, 454, 825
LweI GCATC 1 cut(s) 161
MaeI CTAG 5 cut(s) 128, 140, 578, 681, 738
MaeIII GTNAC 1 cut(s) 800
MalI GATC 5 cut(s) 759, 884, 996, 1047, 1056
MboI GATC 5 cut(s) 757, 882, 994, 1045, 1054
MboII GAAGA 7 cut(s) 81, 116, 202, 314, 509, 653, 847
MflI RGATCY 1 cut(s) 994
MhlI GDGCHC 2 cut(s) 39, 1069
MlsI TGGCCA 1 cut(s) 527
MluNI TGGCCA 1 cut(s) 527
MlyI GAGTC 1 cut(s) 257
MnlI CCTC 7 cut(s) 92, 193, 231, 375, 421, 614, 642
Mox20I TGGCCA 1 cut(s) 527
MroI TCCGGA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 1 cut(s) 527
MseI TTAA 3 cut(s) 357, 420, 1004
MslI CAYNNNNRTG 1 cut(s) 216
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 43, 171, 918
NdeII GATC 5 cut(s) 757, 882, 994, 1045, 1054
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 9 cut(s) 43, 50, 215, 601, 641, 709, 808, 1017, 1055
NlaIV GGNNCC 2 cut(s) 810, 996
PaeR7I CTCGAG 1 cut(s) 618
PagI TCATGA 1 cut(s) 39
PciSI GCTCTTC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 327, 644, 1010
PkrI GCNGC 1 cut(s) 593
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 810, 996
PspPI GGNCC 2 cut(s) 50, 932
PspXI VCTCGAGB 1 cut(s) 618
PsuI RGATCY 1 cut(s) 994
RsaI GTAC 2 cut(s) 411, 870
RsaNI GTAC 2 cut(s) 410, 869
RseI CAYNNNNRTG 1 cut(s) 216
SapI GCTCTTC 1 cut(s) 129
SaqAI TTAA 3 cut(s) 357, 420, 1004
SatI GCNGC 1 cut(s) 592
Sau3AI GATC 5 cut(s) 757, 882, 994, 1045, 1054
Sau96I GGNCC 2 cut(s) 50, 932
ScaI AGTACT 1 cut(s) 870
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 2 cut(s) 39, 1069
SfaNI GCATC 1 cut(s) 161
Sfr274I CTCGAG 1 cut(s) 618
SinI GGWCC 2 cut(s) 50, 932
SlaI CTCGAG 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 216
SmlI CTYRAG 3 cut(s) 618, 878, 1068
SmoI CTYRAG 3 cut(s) 618, 878, 1068
SsiI CCGC 1 cut(s) 592
SspMI CTAG 5 cut(s) 128, 140, 578, 681, 738
StyD4I CCNGG 1 cut(s) 169
TaqI TCGA 4 cut(s) 276, 619, 647, 1104
TatI WGTACW 2 cut(s) 409, 868
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 3 cut(s) 327, 644, 1010
Tru1I TTAA 3 cut(s) 357, 420, 1004
Tru9I TTAA 3 cut(s) 357, 420, 1004
TspDTI ATGAA 9 cut(s) 56, 228, 531, 654, 722, 905, 984, 1002, 1041
VpaK11BI GGWCC 2 cut(s) 50, 932
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 3 cut(s) 416, 624, 1028
XhoI CTCGAG 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 5 cut(s) 128, 140, 578, 681, 738
ZrmI AGTACT 1 cut(s) 870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.