RchiOBHm_Chr7g0219681

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000699
Physical Location & Seq
Reverse (-)
38275454 .. 38279949
4496 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19662

Sequence Viewer

Length: 1806 bp
ATGGCAGAAGTGATCCTTCTCTTCCTTTCAGTGGTCACCCTGCTAAGCTTTCCGACTACAAAAGCCGTCTACATCTACCATAGCTGCCCCAACACCACCGTCTTCACCCCAAACTCCACCTTCCAGTCCAACCTCAACCACCTCCTCTCCACTCTCTCCTCCAACGCCACACGCACTGGCTTCTACAACGCCACCTCCGGCCGTACCCCTAACGACACCGTCTACGGCCTCTTCCTCTGCCGTGGTGACGTCACCACCACCGCTTGCAAGTCCTGCGTGACCACCGCAACCTCCGAGGTCGTGCAGCGCTGCCCGGTCGAGAGAGAGGTTGTGATATGGTACAATGACTGCATGCTACGCTACTCCAACAAGTCCTTCTTCTCTGTCGCCTCCGAGTCGCCGAAAATGTTCGCGTGGAACACCCAAAACGTCAGCATCGAACCGAACCGGTTTAGCCAGGTTTTGGCGAACTTGACGGAGGTTGCCACGGCGGCCGCGAACAGCGGCGAAAAGTTCGCGACGAGAGAGGTGAACTTCACGGGGTTGATTAGCTTGTACAGTTTGGAGCAGTGCACGCAGGACTTGTCTAGTTCTGATTGCAATTGGTGTCTTGTACGAGCCATAGCGGAGTTGCCAACTTGCTGCAGCCGGAAGATAGGAGGACGAGTTCTGTATCCGAGTTGTAATGTTAGGTATGAAGTTTATCCCTTTTATAACTCGACGTCAGCACTGGCGCCGGCGCCAGGGGCTCGGCCTCTTTCTCCGCCAAGCTCAGTGACGAAAGCCGAAAATGATATGACAACTGTTGAATCCTTGCAATTTGACTTGGGAACTGTCGAAGCTGCCACAAAAAAGTTTTCTGCCGATAACATGTTAGGTGAAGGCGGATTTGGTCAAGTTTTCAAGGGAACACTTGATAATGGACAAGAAATAGCTGTGAAGAGGCTGTCAAAAAGCTCTGGACAAGGTGTACGAGAGTTTAAGAACGAGGTTTTGTTGGTAGCAAAGCTTCAACACAGGAATCTTGTAAAGCTTCTAGGGTTTTGCTTGGAAGGAGAAGAAACACTACTTGTCTATGAATATGTGCCCAACAAAAGTCTTGATTATTTTCTATTTGAACAACTGGATTGGTCAAGACGCTGCACCATAATAGGAGGAATCGCTCGAGGAATCCTCTATCTTCATGAAGATTCTAGGCTTCGAGTTATACATCGTGATTTGAAAGCTAGCAATATCTTGTTGGATGCCAATATGAATCCAAAAATTTCAGATTTTGGAATGGCAAGAATGTTTGGAGTTGATGATCAAACACAAGGAAACACACGGAGAATTGTCGGCACATATGGTTACATGGCTCCAGAATATGCTATGGAGGGATTGTATTCTGTAAAATCTGATGTCTTCAGCTTCGGAATACTCTTACTTGAGATCCTAACGGGAAGAAAGAACATTTTAGGCTTTCATCCCACAAATTCTGCACCAACTCTTCTCAGTTTTGCTTGGCAATTATGGAATGAAGGGAAAGTGTTGGAGTTGATGGATCCAATGCTGAAAGATTCCTGCAATCCAAATGAGTTCTTGAGATGCATCCACATTGGATTATTGTGTATTCAAGAAGACGCAAAAAATAGGCCAACCATGTCATCGGTTGTTCTAATGTTAAAGAGTGAAAGTATCAGCCTTTCCAAGCCTGAGCGACCTGCCTTCTTTACAGGAAGATCTATCAATGATCACTGCCATACATTAGGGGATTTACCTAGTTTCTCTGTCAATGCTTTGACAATTTCTGATGATATCCCACGCTGA

Protein Analysis

601

Amino Acids

66.7

Weight (kDa)

6.35

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 29 - 124 4.1e-28 Salt stress response/antifungal
Stress-antifung PF01657 149 - 234 6.4e-10 Salt stress response/antifungal
Pkinase PF00069 288 - 549 4.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 289 - 552 1.7e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61750
fragaria_vesca FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754
malus_domestica MD02G1101300.v1.1 MD05G1031700.v1.1 MD05G1031800.v1.1 MD05G1032400.v1.1 MD05G1032600.v1.1 MD05G1337700.v1.1 MD05G1337900.v1.1 MD05G1340100.v1.1 MD05G1340400.v1.1 MD16G1096700.v1.1
prunus_persica Prupe.4G027600_v2.0.a1 Prupe.8G039200_v2.0.a1
pyrus_communis pycom05g02310 pycom05g02330 pycom05g02340 pycom05g30920
rosa_chinensis RchiOBHm_Chr2g0121501 RchiOBHm_Chr2g0122141 RchiOBHm_Chr5g0004191 RchiOBHm_Chr6g0265241 RchiOBHm_Chr7g0215811 RchiOBHm_Chr7g0215821 RchiOBHm_Chr7g0219681
rosa_laevigata RLG00000002617 RLG00000018618 RLG00000018623 RLG00000031211
rosa_multiflora Rmu_sc0000079.1_g000015 Rmu_sc0000315.1_g000072 Rmu_sc0000315.1_g000093 Rmu_sc0000547.1_g000005 Rmu_sc0000805.1_g000048 Rmu_sc0001851.1_g000024 Rmu_sc0004379.1_g000017 Rmu_sc0004574.1_g000057 Rmu_sc0004689.1_g000004 Rmu_sc0004964.1_g000019 Rmu_sc0007799.1_g000001 Rmu_sc0007868.1_g000012 Rmu_ssc0000359.1_g000013
rosa_roxburghii Rroxscaffold_175G00432180 Rroxscaffold_1G00071500 Rroxscaffold_1G00071530 Rroxscaffold_1G00071780 Rroxscaffold_2G00122400 Rroxscaffold_2G00122420
rosa_rugosa Rorug06G0063800
rosa_samantha Rh2AG290300 Rh2BG301100 Rh2CG279800 Rh5CG038400 Rh5CG040800 Rh5DG036900 Rh6CG182400 Rh6DG173600 Rh7CG314200 Rh7DG297100
rosa_wichuraiana Rw0G021200 Rw0G021210 Rw2G023270 Rw2G023510 Rw5G003650 Rw5G030050 Rw6G011890 Rw7G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AatII GACGTC 2 cut(s) 252, 725
Acc36I ACCTGC 1 cut(s) 1708
AccB1I GGYRCC 2 cut(s) 733, 739
AccB7I CCANNNNNTGG 1 cut(s) 463
AccI GTMKAC 2 cut(s) 69, 222
AccII CGCG 3 cut(s) 413, 497, 518
AciI CCGC 8 cut(s) 261, 285, 491, 495, 504, 626, 764, 885
AclWI GGATC 4 cut(s) 7, 1423, 1535, 1548
AcoI YGGCCR 2 cut(s) 199, 492
AcsI RAATTY 2 cut(s) 1263, 1471
AcuI CTGAAG 1 cut(s) 1387
AcyI GRCGYC 4 cut(s) 249, 722, 734, 740
AfaI GTAC 5 cut(s) 205, 341, 557, 615, 972
AfeI AGCGCT 1 cut(s) 308
AfiI CCNNNNNNNGG 3 cut(s) 31, 463, 743
AflIII ACRYGT 1 cut(s) 870
AgeI ACCGGT 1 cut(s) 447
AgsI TTSAA 6 cut(s) 809, 904, 1013, 1118, 1222, 1613
AjnI CCWGG 2 cut(s) 456, 742
AjuI GAANNNNNNNTTGG 4 cut(s) 359, 391, 873, 905
AloI GAACNNNNNNTCC 2 cut(s) 651, 683
Alw21I GWGCWC 1 cut(s) 575
Alw44I GTGCAC 1 cut(s) 571
AlwI GGATC 4 cut(s) 7, 1423, 1535, 1548
Ama87I CYCGRG 1 cut(s) 1164
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 5 cut(s) 199, 226, 492, 752, 1631
ApaLI GTGCAC 1 cut(s) 571
ApeKI GCWGC 7 cut(s) 84, 304, 309, 642, 645, 842, 1140
ApoI RAATTY 2 cut(s) 1263, 1471
AsiGI ACCGGT 1 cut(s) 447
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 3 cut(s) 309, 736, 742
AsuC2I CCSGG 1 cut(s) 314
AsuHPI GGTGA 6 cut(s) 28, 97, 244, 257, 541, 890
AsuNHI GCTAGC 1 cut(s) 1226
AvaI CYCGRG 1 cut(s) 1164
BaeGI GKGCMC 2 cut(s) 575, 1089
BamHI GGATCC 1 cut(s) 1540
BanI GGYRCC 2 cut(s) 733, 739
BanII GRGCYC 1 cut(s) 751
BarI GAAGNNNNNNTAC 4 cut(s) 993, 1025, 1050, 1082
BbsI GAAGAC 3 cut(s) 94, 1393, 1623
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 7 cut(s) 71, 296, 316, 629, 657, 829, 1127
BccI CCATC 1 cut(s) 1531
BceAI ACGGC 5 cut(s) 50, 186, 225, 241, 504
BciT130I CCWGG 2 cut(s) 458, 744
BciVI GTATCC 1 cut(s) 684
BclI TGATCA 2 cut(s) 1303, 1729
BcnI CCSGG 1 cut(s) 314
BfaI CTAG 5 cut(s) 588, 1037, 1194, 1227, 1758
BfmI CTRYAG 1 cut(s) 643
BfoI RGCGCY 3 cut(s) 310, 737, 743
BfuAI ACCTGC 1 cut(s) 1708
BfuI GTATCC 1 cut(s) 684
BglI GCCNNNNNGGC 1 cut(s) 491
BglII AGATCT 1 cut(s) 1718
BlpI GCTNAGC 1 cut(s) 44
Bme1390I CCNGG 3 cut(s) 314, 458, 744
BmeT110I CYCGRG 1 cut(s) 1164
BmiI GGNNCC 4 cut(s) 735, 741, 1356, 1542
BmrFI CCNGG 3 cut(s) 314, 458, 744
BmsI GCATC 4 cut(s) 444, 1234, 1574, 1596
BmtI GCTAGC 1 cut(s) 1230
BpiI GAAGAC 3 cut(s) 94, 1393, 1623
BplI GAGNNNNNCTC 2 cut(s) 1158, 1190
BpmI CTGGAG 1 cut(s) 1341
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 2 cut(s) 1445, 1600
BpuMI CCSGG 1 cut(s) 314
BsaHI GRCGYC 4 cut(s) 249, 722, 734, 740
BsaJI CCNNGG 4 cut(s) 241, 294, 486, 743
BsaWI WCCGGW 1 cut(s) 447
BsaXI ACNNNNNCTCC 8 cut(s) 131, 161, 179, 209, 651, 681, 1364, 1394
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 463, 743
Bse118I RCCGGY 2 cut(s) 447, 736
Bse1I ACTGG 4 cut(s) 124, 181, 735, 1128
BseBI CCWGG 2 cut(s) 458, 744
BseDI CCNNGG 4 cut(s) 241, 294, 486, 743
BseGI GGATG 3 cut(s) 1249, 1462, 1587
BseLI CCNNNNNNNGG 3 cut(s) 31, 463, 743
BseMII CTCAG 3 cut(s) 786, 1504, 1683
BseNI ACTGG 4 cut(s) 124, 181, 735, 1128
BseRI GAGGAG 2 cut(s) 134, 148
BseSI GKGCMC 2 cut(s) 575, 1089
BseX3I CGGCCG 2 cut(s) 199, 492
BseXI GCAGC 7 cut(s) 71, 296, 316, 629, 657, 829, 1127
BsgI GTGCAG 3 cut(s) 323, 1126, 1461
Bsh1236I CGCG 3 cut(s) 413, 497, 518
Bsh1285I CGRYCG 3 cut(s) 202, 318, 495
BshFI GGCC 5 cut(s) 201, 228, 494, 754, 1633
BshNI GGYRCC 2 cut(s) 733, 739
BshTI ACCGGT 1 cut(s) 447
BsiEI CGRYCG 3 cut(s) 202, 318, 495
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 1 cut(s) 1164
BsiSI CCGG 5 cut(s) 198, 314, 448, 649, 737
BslI CCNNNNNNNGG 3 cut(s) 31, 463, 743
BsnI GGCC 5 cut(s) 201, 228, 494, 754, 1633
BsoBI CYCGRG 1 cut(s) 1164
Bsp1286I GDGCHC 3 cut(s) 575, 751, 1089
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
Bsp1720I GCTNAGC 1 cut(s) 44
Bsp68I TCGCGA 1 cut(s) 518
BspACI CCGC 8 cut(s) 261, 285, 491, 495, 504, 626, 764, 885
BspANI GGCC 5 cut(s) 201, 228, 494, 754, 1633
BspCNI CTCAG 3 cut(s) 785, 1503, 1684
BspFNI CGCG 3 cut(s) 413, 497, 518
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 4 cut(s) 735, 741, 1356, 1542
BspMAI CTGCAG 1 cut(s) 647
BspMI ACCTGC 1 cut(s) 1708
BspOI GCTAGC 1 cut(s) 1230
BspPI GGATC 4 cut(s) 7, 1423, 1535, 1548
BspT107I GGYRCC 2 cut(s) 733, 739
BsrFI RCCGGY 2 cut(s) 447, 736
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 4 cut(s) 124, 181, 735, 1128
BssAI RCCGGY 2 cut(s) 447, 736
BssECI CCNNGG 4 cut(s) 241, 294, 486, 743
BssMI GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
BssNI GRCGYC 4 cut(s) 249, 722, 734, 740
Bst2UI CCWGG 2 cut(s) 458, 744
Bst4CI ACNGT 5 cut(s) 100, 220, 560, 805, 835
Bst6I CTCTTC 4 cut(s) 26, 236, 935, 1491
BstACI GRCGYC 4 cut(s) 249, 722, 734, 740
BstAPI GCANNNNNTGC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 555
BstC8I GCNNGC 5 cut(s) 265, 353, 575, 738, 1228
BstDEI CTNAG 4 cut(s) 44, 772, 1490, 1692
BstDSI CCRYGG 2 cut(s) 241, 486
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 3 cut(s) 1249, 1462, 1587
BstFNI CGCG 3 cut(s) 413, 497, 518
BstH2I RGCGCY 3 cut(s) 310, 737, 743
BstHHI GCGC 3 cut(s) 309, 736, 742
BstKTI GATC 6 cut(s) 15, 1306, 1431, 1543, 1721, 1732
BstMBI GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
BstMCI CGRYCG 3 cut(s) 202, 318, 495
BstMWI GCNNNNNNNGC 5 cut(s) 273, 357, 491, 574, 746
BstNI CCWGG 2 cut(s) 458, 744
BstNSI RCATGY 2 cut(s) 355, 874
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 3 cut(s) 312, 456, 742
BstSFI CTRYAG 1 cut(s) 643
BstSLI GKGCMC 2 cut(s) 575, 1089
BstUI CGCG 3 cut(s) 413, 497, 518
BstV1I GCAGC 7 cut(s) 71, 296, 316, 629, 657, 829, 1127
BstV2I GAAGAC 3 cut(s) 94, 1393, 1623
BstX2I RGATCY 3 cut(s) 1428, 1540, 1718
BstYI RGATCY 3 cut(s) 1428, 1540, 1718
BstZI CGGCCG 2 cut(s) 199, 492
BsuI GTATCC 1 cut(s) 684
BsuRI GGCC 5 cut(s) 201, 228, 494, 754, 1633
BtgI CCRYGG 2 cut(s) 241, 486
BtsCI GGATG 3 cut(s) 1249, 1462, 1587
BtsI GCAGTG 2 cut(s) 575, 1732
BtsIMutI CAGTG 6 cut(s) 36, 174, 575, 728, 780, 1732
BtuMI TCGCGA 1 cut(s) 518
BveI ACCTGC 1 cut(s) 1708
Cac8I GCNNGC 5 cut(s) 265, 353, 575, 738, 1228
CciI TCATGA 1 cut(s) 1183
CciNI GCGGCCGC 1 cut(s) 492
CfoI GCGC 3 cut(s) 309, 736, 742
Cfr10I RCCGGY 2 cut(s) 447, 736
CseI GACGC 2 cut(s) 1146, 1628
Csp6I GTAC 5 cut(s) 204, 340, 556, 614, 971
CspAI ACCGGT 1 cut(s) 447
CviAII CATG 5 cut(s) 352, 871, 1184, 1351, 1639
CviQI GTAC 5 cut(s) 204, 340, 556, 614, 971
DdeI CTNAG 4 cut(s) 44, 772, 1490, 1692
DinI GGCGCC 2 cut(s) 735, 741
DpnI GATC 6 cut(s) 14, 1305, 1430, 1542, 1720, 1731
DpnII GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
EaeI YGGCCR 2 cut(s) 199, 492
EagI CGGCCG 2 cut(s) 199, 492
Eam1104I CTCTTC 4 cut(s) 26, 236, 935, 1491
EarI CTCTTC 4 cut(s) 26, 236, 935, 1491
EciI GGCGGA 2 cut(s) 753, 900
EclXI CGGCCG 2 cut(s) 199, 492
Eco24I GRGCYC 1 cut(s) 751
Eco32I GATATC 1 cut(s) 1795
Eco47III AGCGCT 1 cut(s) 308
Eco52I CGGCCG 2 cut(s) 199, 492
Eco57I CTGAAG 1 cut(s) 1387
Eco88I CYCGRG 1 cut(s) 1164
Eco91I GGTNACC 1 cut(s) 34
EcoO65I GGTNACC 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 456, 742
EcoRV GATATC 1 cut(s) 1795
EcoT22I ATGCAT 1 cut(s) 1589
EcoT38I GRGCYC 1 cut(s) 751
EgeI GGCGCC 2 cut(s) 735, 741
EheI GGCGCC 2 cut(s) 735, 741
FaeI CATG 5 cut(s) 355, 874, 1187, 1354, 1642
FalI AAGNNNNNCTT 3 cut(s) 32, 362, 394
FatI CATG 5 cut(s) 351, 870, 1183, 1350, 1638
FauNDI CATATG 1 cut(s) 1342
FbaI TGATCA 2 cut(s) 1303, 1729
FblI GTMKAC 2 cut(s) 69, 222
FokI GGATG 3 cut(s) 1256, 1449, 1574
FriOI GRGCYC 1 cut(s) 751
FspBI CTAG 5 cut(s) 588, 1037, 1194, 1227, 1758
GlaI GCGC 3 cut(s) 308, 735, 741
GsuI CTGGAG 1 cut(s) 1341
HaeII RGCGCY 3 cut(s) 310, 737, 743
HaeIII GGCC 5 cut(s) 201, 228, 494, 754, 1633
HapII CCGG 5 cut(s) 198, 314, 448, 649, 737
HgaI GACGC 2 cut(s) 1146, 1628
HhaI GCGC 3 cut(s) 309, 736, 742
Hin1I GRCGYC 4 cut(s) 249, 722, 734, 740
Hin1II CATG 5 cut(s) 355, 874, 1187, 1354, 1642
Hin6I GCGC 3 cut(s) 307, 734, 740
HinP1I GCGC 3 cut(s) 307, 734, 740
HindIII AAGCTT 3 cut(s) 46, 1007, 1031
HinfI GANTC 8 cut(s) 395, 809, 1021, 1158, 1170, 1190, 1255, 1556
HpaII CCGG 5 cut(s) 198, 314, 448, 649, 737
HphI GGTGA 6 cut(s) 28, 97, 244, 257, 541, 890
Hpy166II GTNNAC 5 cut(s) 70, 223, 532, 573, 971
Hpy188I TCNGA 9 cut(s) 54, 295, 394, 595, 678, 1270, 1396, 1412, 1789
Hpy8I GTNNAC 5 cut(s) 70, 223, 532, 573, 971
Hpy99I CGWCG 2 cut(s) 523, 724
HpyAV CCTTC 7 cut(s) 26, 130, 385, 875, 1046, 1511, 1714
HpyCH4III ACNGT 5 cut(s) 100, 220, 560, 805, 835
HpyCH4IV ACGT 3 cut(s) 249, 429, 722
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 357, 491, 574, 746
HpyF3I CTNAG 4 cut(s) 44, 772, 1490, 1692
HpySE526I ACGT 3 cut(s) 249, 429, 722
Hsp92I GRCGYC 4 cut(s) 249, 722, 734, 740
Hsp92II CATG 5 cut(s) 355, 874, 1187, 1354, 1642
HspAI GCGC 3 cut(s) 307, 734, 740
KasI GGCGCC 2 cut(s) 733, 739
KroI GCCGGC 1 cut(s) 736
KroNI GCCGGC 1 cut(s) 738
Ksp22I TGATCA 2 cut(s) 1303, 1729
Kzo9I GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
LmnI GCTCC 2 cut(s) 565, 1360
Lsp1109I GCAGC 7 cut(s) 71, 296, 316, 629, 657, 829, 1127
LweI GCATC 4 cut(s) 444, 1234, 1574, 1596
MaeI CTAG 5 cut(s) 588, 1037, 1194, 1227, 1758
MaeII ACGT 3 cut(s) 249, 429, 722
MaeIII GTNAC 6 cut(s) 34, 245, 250, 277, 775, 1346
MalI GATC 6 cut(s) 14, 1305, 1430, 1542, 1720, 1731
MboI GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
MfeI CAATTG 1 cut(s) 601
MflI RGATCY 3 cut(s) 1428, 1540, 1718
MhlI GDGCHC 3 cut(s) 575, 751, 1089
MluCI AATT 7 cut(s) 601, 818, 1263, 1329, 1471, 1505, 1782
Mly113I GGCGCC 2 cut(s) 734, 740
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 6 cut(s) 77, 153, 186, 390, 1221, 1509
Mph1103I ATGCAT 1 cut(s) 1589
MreI CGCCGGCG 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 2 cut(s) 981, 1661
MspA1I CMGCKG 1 cut(s) 504
MspI CCGG 5 cut(s) 198, 314, 448, 649, 737
MspR9I CCNGG 3 cut(s) 314, 458, 744
MunI CAATTG 1 cut(s) 601
MvaI CCWGG 2 cut(s) 458, 744
MvnI CGCG 3 cut(s) 413, 497, 518
MwoI GCNNNNNNNGC 5 cut(s) 273, 357, 491, 574, 746
NaeI GCCGGC 1 cut(s) 738
NarI GGCGCC 2 cut(s) 734, 740
NciI CCSGG 1 cut(s) 314
NdeI CATATG 1 cut(s) 1342
NdeII GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
NgoMIV GCCGGC 1 cut(s) 736
NheI GCTAGC 1 cut(s) 1226
NlaIII CATG 5 cut(s) 355, 874, 1187, 1354, 1642
NlaIV GGNNCC 4 cut(s) 735, 741, 1356, 1542
NmeAIII GCCGAG 1 cut(s) 730
NmuCI GTSAC 5 cut(s) 34, 245, 250, 277, 775
NotI GCGGCCGC 1 cut(s) 492
NruI TCGCGA 1 cut(s) 518
NsiI ATGCAT 1 cut(s) 1589
NspI RCATGY 2 cut(s) 355, 874
PaeI GCATGC 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 1164
PagI TCATGA 1 cut(s) 1183
PciI ACATGT 1 cut(s) 870
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 494
PdiI GCCGGC 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 7 cut(s) 809, 1021, 1158, 1170, 1190, 1255, 1556
PflFI GACNNNGTC 1 cut(s) 218
PflMI CCANNNNNTGG 1 cut(s) 463
PinAI ACCGGT 1 cut(s) 447
PleI GAGTC 1 cut(s) 403
PluTI GGCGCC 2 cut(s) 737, 743
PpsI GAGTC 1 cut(s) 403
PscI ACATGT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 714
Psp6I CCWGG 2 cut(s) 456, 742
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 456, 742
PspN4I GGNNCC 4 cut(s) 735, 741, 1356, 1542
PspXI VCTCGAGB 1 cut(s) 1164
PstI CTGCAG 1 cut(s) 647
PsuI RGATCY 3 cut(s) 1428, 1540, 1718
PsyI GACNNNGTC 1 cut(s) 218
RruI TCGCGA 1 cut(s) 518
RsaI GTAC 5 cut(s) 205, 341, 557, 615, 972
RsaNI GTAC 5 cut(s) 204, 340, 556, 614, 971
SaqAI TTAA 2 cut(s) 981, 1661
Sau3AI GATC 6 cut(s) 12, 1303, 1428, 1540, 1718, 1729
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 3 cut(s) 314, 458, 744
SduI GDGCHC 3 cut(s) 575, 751, 1089
SfaNI GCATC 4 cut(s) 444, 1234, 1574, 1596
SfcI CTRYAG 1 cut(s) 643
SfoI GGCGCC 2 cut(s) 735, 741
Sfr274I CTCGAG 1 cut(s) 1164
SgrAI CRCCGGYG 1 cut(s) 736
SlaI CTCGAG 1 cut(s) 1164
SmlI CTYRAG 3 cut(s) 1164, 1424, 1579
SmoI CTYRAG 3 cut(s) 1164, 1424, 1579
SphI GCATGC 1 cut(s) 355
Sse9I AATT 7 cut(s) 601, 818, 1263, 1329, 1471, 1505, 1782
SsiI CCGC 8 cut(s) 261, 285, 491, 495, 504, 626, 764, 885
SspDI GGCGCC 2 cut(s) 733, 739
SspMI CTAG 5 cut(s) 588, 1037, 1194, 1227, 1758
StyD4I CCNGG 3 cut(s) 312, 456, 742
TaaI ACNGT 5 cut(s) 100, 220, 560, 805, 835
TaiI ACGT 3 cut(s) 252, 432, 725
TaqI TCGA 6 cut(s) 318, 438, 719, 837, 1165, 1201
TasI AATT 7 cut(s) 601, 818, 1263, 1329, 1471, 1505, 1782
TatI WGTACW 1 cut(s) 555
TauI GCSGC 3 cut(s) 494, 497, 507
TfiI GAWTC 7 cut(s) 809, 1021, 1158, 1170, 1190, 1255, 1556
Tru1I TTAA 2 cut(s) 981, 1661
Tru9I TTAA 2 cut(s) 981, 1661
TscAI CASTG 6 cut(s) 36, 181, 575, 735, 780, 1739
TseFI GTSAC 5 cut(s) 34, 245, 250, 277, 775
TseI GCWGC 7 cut(s) 84, 304, 309, 642, 645, 842, 1140
Tsp45I GTSAC 5 cut(s) 34, 245, 250, 277, 775
TspDTI ATGAA 7 cut(s) 711, 1092, 1172, 1200, 1268, 1451, 1530
TspGWI ACGGA 2 cut(s) 491, 1339
TspRI CASTG 6 cut(s) 36, 181, 575, 735, 780, 1739
Tth111I GACNNNGTC 1 cut(s) 218
Van91I CCANNNNNTGG 1 cut(s) 463
VneI GTGCAC 1 cut(s) 571
XapI RAATTY 2 cut(s) 1263, 1471
XceI RCATGY 2 cut(s) 355, 874
XhoI CTCGAG 1 cut(s) 1164
XmiI GTMKAC 2 cut(s) 69, 222
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 5 cut(s) 588, 1037, 1194, 1227, 1758
ZraI GACGTC 2 cut(s) 250, 723
Zsp2I ATGCAT 1 cut(s) 1589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.