MD16G1096700.v1.1

Salt stress response/antifungal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000699
Physical Location & Seq
Reverse (-)
6741636 .. 6742130
495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1096700.v1.1.491

Sequence Viewer

Length: 495 bp
ATGTTGCGCTACTCAGACGAGTTTTTCTTTTCCAGACCGGACCACATCCCTTTAAAGTACTTGAGGAACAAGGAGAAGGTAACGGCCAACGAAGAAAGCCGCTTTTACGAGGTACTGGAGGCGAGTGTGAATGAGTTGGCCTTTCAGGCTTCCAATAAAAGCAATATGTTTGCGACCAAAAAAAGGGACGTTTCGGGGTCCATTTCGCTGCAAGTGCTTGGGCAGTGCACGCAGGACTTGTCCTCTGGAGATTGCTATTGGTGTCTTCAGGGAGCCATAGCAGAAGTTGTTCCCAGAATACAAGAAGGCGGACGACTTCTACGTCCCAGTTGCCGTCTTACGATTGAAGTTTCCCGCTACTTATACCTCCCCAACCCCTCCTCCTCACCGCCGCTTCCTCCTCCGCCAGGCTCCAACAGGGCAAGGCCTAAAGGTGCCTTAAACTTATTAATTCGCTTCTGTGTCTATCATCTCCATCTCCATCACCACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Stress-antifung

Protein Analysis

165

Amino Acids

18.69

Weight (kDa)

9.02

Isoelectric Point (pI)

60.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stress-antifung PF01657 28 - 116 2.2e-07 Salt stress response/antifungal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61750
fragaria_vesca FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754
malus_domestica MD02G1101300.v1.1 MD05G1031700.v1.1 MD05G1031800.v1.1 MD05G1032400.v1.1 MD05G1032600.v1.1 MD05G1337700.v1.1 MD05G1337900.v1.1 MD05G1340100.v1.1 MD05G1340400.v1.1 MD16G1096700.v1.1
prunus_persica Prupe.4G027600_v2.0.a1 Prupe.8G039200_v2.0.a1
pyrus_communis pycom05g02310 pycom05g02330 pycom05g02340 pycom05g30920
rosa_chinensis RchiOBHm_Chr2g0121501 RchiOBHm_Chr2g0122141 RchiOBHm_Chr5g0004191 RchiOBHm_Chr6g0265241 RchiOBHm_Chr7g0215811 RchiOBHm_Chr7g0215821 RchiOBHm_Chr7g0219681
rosa_laevigata RLG00000002617 RLG00000018618 RLG00000018623 RLG00000031211
rosa_multiflora Rmu_sc0000079.1_g000015 Rmu_sc0000315.1_g000072 Rmu_sc0000315.1_g000093 Rmu_sc0000547.1_g000005 Rmu_sc0000805.1_g000048 Rmu_sc0001851.1_g000024 Rmu_sc0004379.1_g000017 Rmu_sc0004574.1_g000057 Rmu_sc0004689.1_g000004 Rmu_sc0004964.1_g000019 Rmu_sc0007799.1_g000001 Rmu_sc0007868.1_g000012 Rmu_ssc0000359.1_g000013
rosa_roxburghii Rroxscaffold_175G00432180 Rroxscaffold_1G00071500 Rroxscaffold_1G00071530 Rroxscaffold_1G00071780 Rroxscaffold_2G00122400 Rroxscaffold_2G00122420
rosa_rugosa Rorug06G0063800
rosa_samantha Rh2AG290300 Rh2BG301100 Rh2CG279800 Rh5CG038400 Rh5CG040800 Rh5DG036900 Rh6CG182400 Rh6DG173600 Rh7CG314200 Rh7DG297100
rosa_wichuraiana Rw0G021200 Rw0G021210 Rw2G023270 Rw2G023510 Rw5G003650 Rw5G030050 Rw6G011890 Rw7G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 434
AciI CCGC 6 cut(s) 100, 309, 355, 389, 392, 404
AcoI YGGCCR 1 cut(s) 84
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 2 cut(s) 59, 114
AfiI CCNNNNNNNGG 2 cut(s) 183, 407
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 1 cut(s) 406
Alw21I GWGCWC 1 cut(s) 230
Alw44I GTGCAC 1 cut(s) 226
AoxI GGCC 3 cut(s) 84, 138, 425
ApaLI GTGCAC 1 cut(s) 226
ApeKI GCWGC 1 cut(s) 208
AseI ATTAAT 1 cut(s) 449
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 40, 198
AsuHPI GGTGA 2 cut(s) 378, 476
AvaII GGWCC 2 cut(s) 40, 198
BaeGI GKGCMC 1 cut(s) 230
BanI GGYRCC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 195
BccI CCATC 2 cut(s) 483, 489
BceAI ACGGC 2 cut(s) 99, 318
BciT130I CCWGG 1 cut(s) 408
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 3 cut(s) 100, 209, 392
BlsI GCNGC 3 cut(s) 101, 210, 393
BmcAI AGTACT 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 408
Bme18I GGWCC 2 cut(s) 40, 198
BmgT120I GGNCC 2 cut(s) 40, 198
BmiI GGNNCC 4 cut(s) 199, 274, 412, 436
BmrFI CCNGG 1 cut(s) 408
BmrI ACTGGG 1 cut(s) 321
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 137, 267
BpuEI CTTGAG 1 cut(s) 82
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 2 cut(s) 365, 395
Bsc4I CCNNNNNNNGG 2 cut(s) 183, 407
Bse1I ACTGG 2 cut(s) 120, 327
BseBI CCWGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 2 cut(s) 183, 407
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 2 cut(s) 120, 327
BseRI GAGGAG 3 cut(s) 370, 373, 390
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 1 cut(s) 195
BshFI GGCC 3 cut(s) 86, 140, 427
BshNI GGYRCC 1 cut(s) 434
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 2 cut(s) 200, 309
BslI CCNNNNNNNGG 2 cut(s) 183, 407
BsmFI GGGAC 2 cut(s) 200, 309
BsnI GGCC 3 cut(s) 86, 140, 427
Bsp1286I GDGCHC 1 cut(s) 230
BspACI CCGC 6 cut(s) 100, 309, 355, 389, 392, 404
BspANI GGCC 3 cut(s) 86, 140, 427
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 4 cut(s) 199, 274, 412, 436
BspT107I GGYRCC 1 cut(s) 434
BsrI ACTGG 2 cut(s) 120, 327
Bst2UI CCWGG 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 1 cut(s) 13
BstENI CCTNNNNNAGG 1 cut(s) 405
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 9
BstMWI GCNNNNNNNGC 3 cut(s) 146, 214, 229
BstNI CCWGG 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 406
BstSLI GKGCMC 1 cut(s) 230
BstV1I GCAGC 1 cut(s) 195
BstV2I GAAGAC 1 cut(s) 257
BsuRI GGCC 3 cut(s) 86, 140, 427
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 9
Cfr13I GGNCC 2 cut(s) 40, 198
Csp6I GTAC 2 cut(s) 58, 113
CviJI RGCY 7 cut(s) 86, 99, 140, 149, 275, 411, 427
CviKI_1 RGCY 7 cut(s) 86, 99, 140, 149, 275, 411, 427
CviQI GTAC 2 cut(s) 58, 113
DdeI CTNAG 1 cut(s) 13
DraI TTTAAA 1 cut(s) 54
DrdI GACNNNNNNGTC 1 cut(s) 321
DseDI GACNNNNNNGTC 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 84
EciI GGCGGA 2 cut(s) 324, 393
Eco147I AGGCCT 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 40, 198
Eco57I CTGAAG 1 cut(s) 251
EcoNI CCTNNNNNAGG 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 406
FaiI YATR 3 cut(s) 167, 278, 364
FaqI GGGAC 2 cut(s) 200, 309
FauI CCCGC 1 cut(s) 362
Fnu4HI GCNGC 3 cut(s) 100, 209, 392
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 3 cut(s) 100, 209, 392
GlaI GCGC 1 cut(s) 8
GluI GCNGC 3 cut(s) 100, 209, 392
GsuI CTGGAG 2 cut(s) 137, 267
HaeIII GGCC 3 cut(s) 86, 140, 427
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 7
HinP1I GCGC 1 cut(s) 7
HpaII CCGG 1 cut(s) 38
HphI GGTGA 2 cut(s) 378, 476
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 33, 246
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 2 cut(s) 70, 299
HpyCH4IV ACGT 2 cut(s) 189, 322
HpyCH4V TGCA 2 cut(s) 211, 228
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 214, 229
HpyF3I CTNAG 1 cut(s) 13
HpySE526I ACGT 2 cut(s) 189, 322
HspAI GCGC 1 cut(s) 7
LmnI GCTCC 2 cut(s) 272, 416
Lsp1109I GCAGC 1 cut(s) 195
MaeII ACGT 2 cut(s) 189, 322
MaeIII GTNAC 1 cut(s) 79
MboII GAAGA 2 cut(s) 104, 257
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 1 cut(s) 450
MmeI TCCRAC 1 cut(s) 438
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 3 cut(s) 53, 440, 449
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 408
MwoI GCNNNNNNNGC 3 cut(s) 146, 214, 229
NlaIV GGNNCC 4 cut(s) 199, 274, 412, 436
PceI AGGCCT 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 288
PkrI GCNGC 3 cut(s) 101, 210, 393
PshBI ATTAAT 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 406
PspGI CCWGG 1 cut(s) 406
PspN4I GGNNCC 4 cut(s) 199, 274, 412, 436
PspPI GGNCC 2 cut(s) 40, 198
RsaI GTAC 2 cut(s) 59, 114
RsaNI GTAC 2 cut(s) 58, 113
SaqAI TTAA 3 cut(s) 53, 440, 449
SatI GCNGC 3 cut(s) 100, 209, 392
Sau96I GGNCC 2 cut(s) 40, 198
ScaI AGTACT 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 408
SduI GDGCHC 1 cut(s) 230
SetI ASST 6 cut(s) 81, 114, 192, 325, 369, 436
SinI GGWCC 2 cut(s) 40, 198
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 1 cut(s) 450
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 6 cut(s) 100, 309, 355, 389, 392, 404
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 406
TaiI ACGT 2 cut(s) 192, 325
TasI AATT 1 cut(s) 450
TatI WGTACW 1 cut(s) 57
TauI GCSGC 2 cut(s) 102, 394
Tru1I TTAA 3 cut(s) 53, 440, 449
Tru9I TTAA 3 cut(s) 53, 440, 449
TscAI CASTG 1 cut(s) 230
TseI GCWGC 1 cut(s) 208
TspRI CASTG 1 cut(s) 230
VneI GTGCAC 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 40, 198
VspI ATTAAT 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 288
ZrmI AGTACT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.