Rorug06G0063800

Cysteine-rich receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000699
Physical Location & Seq
Reverse (-)
8528559 .. 8531142
2584 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0063800.1

Sequence Viewer

Length: 1791 bp
ATGGCAGTTGACTCGTCCCTTCCTTCTCCACTCGGGCCACCGGCCTGTGAGAAAGACGCCAAGGCCTTGCAATTCATCGAACAAACCACCCGAAACGCCGACGCCGTCCAGGAGACGGTGCTCGCCGAGATTCTCAGCCGAAACGCCGAGACCGAATACCTCCGACGCTACAAGCTCGGTGGCGCCACTGACCGTAAATCCTTTAAATCCAATCTTCCAGTCATCACTTACGAGAATCTCCAGCCGGAAATCCAAAGGATCGCCAATGGAGATCGCTCCCCTATCCTCTCGGCTCATCCCATCTCTGAGTTTCTCACCAGCTCTGGTACTTCAGCGGGTGAGAGGAAGCTGATGCCAACGATTCAGGAGGAGTGGGATCGTCGTCAATCACTCTACAACCTTCTCATGCCAGTCATGAACCTTTGTGTGCCTGGTTTGGACCAAGGAAAGGGATTATACTTTCTGTTCGTCAAGTCCGAATCAAAGACTCCGGGCGGACTCTTGGCCCGACCCGTTTTGACCAGCTACTACAAAAGCGAGCATTTCAAGACTCGCCCGTACGATCCCTACAATGTCTACACCAGCCCCAACGAGGCCATCCTCTGCCCCGACTCGTTTCAATCCATGTACACTCAAATGCTGTGTGGCCTCCTCGAGCGCCACCAAGTCCTCCGCCTCGGCGCCGTTTTCGCCTCCGGTCTCCTCCGCGCCATCCACTTCCTCCAGCTCAACTGGCAGCAACTCGCCGCCGACATCCGCACCGGAACACTCAACCCCAAAATTACCGACCCGGAGTTGAGGGGATGCATGGAGCGGAAACTGAAACCCGAGCCTGAACTTGCGGAGTTCGTTGCCGAACAATGCGGGGACGAGAATTGGCAGGGAATCATAACCCGAATTTGGCCCAGTACAAAATATCTCGACGTCATCGTCACCGGAGCTATGGCCCAGTATATTCCGACTTTGGATTACTACAGCGGCGGCTTACCCATGGCCTGCACCATGTACGCTTCGTCCGAGTGTTACTTCGGACTCAACCTGAACCCGATGTGCAGACCTTCAGAAGTTTCATACACAATTATGCCAAACATGGCTTACTTCGAGTTCCTCCCCTACGAGCCCAACTCAGTGAGTCTTGACCCGACCCGACTCGTTGACATGGTCGACGTGGTGGTCGGAAAAGAATACGAGCTCGTTATTACCACTTACGCCGGACTCTACCGCTACCGCGTCGGCGACATTCTCCGGGTCACCGGGTTCCACAACTCGGCGCCGCAGTTCCACTTCGTGCGGCGGAAGAACGTCCTGCTCAGCATTGACTCCGACAAGACCGACGAGGCGGAGCTCCAGCTCGCCGTCGACAACGCCACCAAGCTCGTCCTTGAGAAGCTCAACGCCAGCGTGGTGGAGTACACCAGCTTCGCCGACACCAAGACCATCCCGGGCCACTACGTCATCTACTGGGAGCTTCTAGTCAAGGACTCGGCCGAGTCGCCCAACTCGGAATCGCTGAGTCAGTGCTGCTTGGCCATGGAGGAATCGCTCAACTCGGTTTACCGGCAGTGCCGGGTGGCGGACAACTCGATCGGGCCGTTGGAGATACGTGTGGTGAAGAGCGGCACGTTCGAGGAGTTGATGGACTACGCGATCTCGAGAGGCGCGTCGATTAACCAGTATAAGGTGCCGCGGTGTGTGAATTTTACCCCGATAATGGAGTTACTGGACTCCAGGGTGGTTTCCAAGCATTTTAGCCCTTCTTTACCGCATTGGACCCCGGAAAGGAGACGGTAA

Protein Analysis

596

Amino Acids

67.28

Weight (kDa)

5.59

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GH3 PF03321 23 - 331 2.5e-120 GH3 family N-terminal domain
GH3_M PF23571 358 - 432 2.7e-30 GH3 family central domain
GH3_C PF23572 448 - 567 4.9e-40 GH3 family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000699)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61750
fragaria_vesca FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754 FvH4_3g02754
malus_domestica MD02G1101300.v1.1 MD05G1031700.v1.1 MD05G1031800.v1.1 MD05G1032400.v1.1 MD05G1032600.v1.1 MD05G1337700.v1.1 MD05G1337900.v1.1 MD05G1340100.v1.1 MD05G1340400.v1.1 MD16G1096700.v1.1
prunus_persica Prupe.4G027600_v2.0.a1 Prupe.8G039200_v2.0.a1
pyrus_communis pycom05g02310 pycom05g02330 pycom05g02340 pycom05g30920
rosa_chinensis RchiOBHm_Chr2g0121501 RchiOBHm_Chr2g0122141 RchiOBHm_Chr5g0004191 RchiOBHm_Chr6g0265241 RchiOBHm_Chr7g0215811 RchiOBHm_Chr7g0215821 RchiOBHm_Chr7g0219681
rosa_laevigata RLG00000002617 RLG00000018618 RLG00000018623 RLG00000031211
rosa_multiflora Rmu_sc0000079.1_g000015 Rmu_sc0000315.1_g000072 Rmu_sc0000315.1_g000093 Rmu_sc0000547.1_g000005 Rmu_sc0000805.1_g000048 Rmu_sc0001851.1_g000024 Rmu_sc0004379.1_g000017 Rmu_sc0004574.1_g000057 Rmu_sc0004689.1_g000004 Rmu_sc0004964.1_g000019 Rmu_sc0007799.1_g000001 Rmu_sc0007868.1_g000012 Rmu_ssc0000359.1_g000013
rosa_roxburghii Rroxscaffold_175G00432180 Rroxscaffold_1G00071500 Rroxscaffold_1G00071530 Rroxscaffold_1G00071780 Rroxscaffold_2G00122400 Rroxscaffold_2G00122420
rosa_rugosa Rorug06G0063800
rosa_samantha Rh2AG290300 Rh2BG301100 Rh2CG279800 Rh5CG038400 Rh5CG040800 Rh5DG036900 Rh6CG182400 Rh6DG173600 Rh7CG314200 Rh7DG297100
rosa_wichuraiana Rw0G021200 Rw0G021210 Rw2G023270 Rw2G023510 Rw5G003650 Rw5G030050 Rw6G011890 Rw7G024980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 929, 1172
AatII GACGTC 1 cut(s) 927
AccB1I GGYRCC 4 cut(s) 182, 680, 1270, 1681
AccBSI CCGCTC 2 cut(s) 814, 1617
AccI GTMKAC 3 cut(s) 576, 1164, 1359
AccII CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
AclWI GGATC 3 cut(s) 266, 384, 557
AcoI YGGCCR 2 cut(s) 1485, 1527
AcsI RAATTY 2 cut(s) 897, 1696
AcuI CTGAAG 2 cut(s) 315, 1044
AcyI GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
AdeI CACNNNGTG 1 cut(s) 1288
AfaI GTAC 6 cut(s) 328, 560, 629, 910, 1007, 1412
AfiI CCNNNNNNNGG 9 cut(s) 115, 448, 592, 900, 1267, 1573, 1678, 1711, 1779
AflIII ACRYGT 1 cut(s) 1603
AgsI TTSAA 2 cut(s) 547, 620
AjiI CACGTC 1 cut(s) 1168
AjnI CCWGG 3 cut(s) 108, 430, 1727
Alw21I GWGCWC 3 cut(s) 123, 1194, 1347
Alw26I GTCTC 4 cut(s) 107, 143, 704, 1777
AlwI GGATC 3 cut(s) 266, 384, 557
Ama87I CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
ApeKI GCWGC 2 cut(s) 736, 1521
ApoI RAATTY 2 cut(s) 897, 1696
AspLEI GCGC 6 cut(s) 185, 660, 683, 710, 1273, 1661
AspS9I GGNCC 8 cut(s) 35, 439, 505, 903, 946, 1444, 1589, 1770
AsuC2I CCSGG 8 cut(s) 492, 791, 1247, 1255, 1442, 1443, 1568, 1775
AsuHPI GGTGA 5 cut(s) 307, 350, 925, 1243, 1621
AvaI CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
AvaII GGWCC 2 cut(s) 439, 1770
BalI TGGCCA 1 cut(s) 1529
BanI GGYRCC 4 cut(s) 182, 680, 1270, 1681
BanII GRGCYC 3 cut(s) 1122, 1194, 1347
BarI GAAGNNNNNNTAC 2 cut(s) 1403, 1435
Bbv12I GWGCWC 3 cut(s) 123, 1194, 1347
BbvI GCAGC 2 cut(s) 748, 1508
BccI CCATC 5 cut(s) 308, 605, 719, 1445, 1630
BceAI ACGGC 4 cut(s) 89, 668, 1340, 1576
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 3 cut(s) 110, 432, 1729
BcnI CCSGG 8 cut(s) 492, 791, 1247, 1255, 1442, 1443, 1568, 1775
BcoDI GTCTC 4 cut(s) 107, 143, 704, 1777
BfaI CTAG 1 cut(s) 1472
BfmI CTRYAG 1 cut(s) 973
BfoI RGCGCY 4 cut(s) 186, 661, 684, 1274
BisI GCNGC 9 cut(s) 737, 747, 979, 982, 1274, 1292, 1522, 1618, 1685
BlpI GCTNAGC 1 cut(s) 1310
BlsI GCNGC 9 cut(s) 738, 748, 980, 983, 1275, 1293, 1523, 1619, 1686
Bme18I GGWCC 2 cut(s) 439, 1770
BmeT110I CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
BmgBI CACGTC 1 cut(s) 1168
BmgT120I GGNCC 8 cut(s) 35, 439, 505, 903, 946, 1444, 1589, 1770
BmiI GGNNCC 6 cut(s) 184, 682, 1259, 1272, 1683, 1772
BmrI ACTGGG 3 cut(s) 900, 943, 1471
BmsI GCATC 2 cut(s) 342, 794
BmuI ACTGGG 3 cut(s) 900, 943, 1471
BplI GAGNNNNNCTC 2 cut(s) 1109, 1141
BpmI CTGGAG 4 cut(s) 224, 707, 1331, 1711
Bpu1102I GCTNAGC 1 cut(s) 1310
BpuEI CTTGAG 1 cut(s) 1403
BpuMI CCSGG 8 cut(s) 492, 791, 1247, 1255, 1442, 1443, 1568, 1775
BsaAI YACGTR 1 cut(s) 1604
BsaHI GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
BsaI GGTCTC 2 cut(s) 143, 704
BsaJI CCNNGG 9 cut(s) 60, 442, 676, 990, 1441, 1530, 1685, 1728, 1773
BsaWI WCCGGW 3 cut(s) 695, 761, 935
Bsc4I CCNNNNNNNGG 9 cut(s) 115, 448, 592, 900, 1267, 1573, 1678, 1711, 1779
Bse118I RCCGGY 2 cut(s) 40, 1557
Bse1I ACTGG 8 cut(s) 218, 410, 737, 906, 949, 1466, 1672, 1725
BseBI CCWGG 3 cut(s) 110, 432, 1729
BseDI CCNNGG 9 cut(s) 60, 442, 676, 990, 1441, 1530, 1685, 1728, 1773
BseGI GGATG 6 cut(s) 295, 597, 711, 753, 809, 1437
BseLI CCNNNNNNNGG 9 cut(s) 115, 448, 592, 900, 1267, 1573, 1678, 1711, 1779
BseMII CTCAG 5 cut(s) 148, 297, 1140, 1324, 1502
BseNI ACTGG 8 cut(s) 218, 410, 737, 906, 949, 1466, 1672, 1725
BseRI GAGGAG 4 cut(s) 383, 641, 692, 1643
BseX3I CGGCCG 1 cut(s) 1485
BseXI GCAGC 2 cut(s) 748, 1508
BsgI GTGCAG 2 cut(s) 982, 1072
Bsh1236I CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
Bsh1285I CGRYCG 2 cut(s) 1488, 1587
BshNI GGYRCC 4 cut(s) 182, 680, 1270, 1681
BsiEI CGRYCG 2 cut(s) 1488, 1587
BsiHKAI GWGCWC 3 cut(s) 123, 1194, 1347
BsiHKCI CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
BsiWI CGTACG 1 cut(s) 558
BslFI GGGAC 1 cut(s) 881
BslI CCNNNNNNNGG 9 cut(s) 115, 448, 592, 900, 1267, 1573, 1678, 1711, 1779
BsmAI GTCTC 4 cut(s) 107, 143, 704, 1777
BsmBI CGTCTC 2 cut(s) 107, 1777
BsmFI GGGAC 1 cut(s) 881
Bso31I GGTCTC 2 cut(s) 143, 704
BsoBI CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
Bsp1286I GDGCHC 4 cut(s) 123, 1122, 1194, 1347
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
Bsp1720I GCTNAGC 1 cut(s) 1310
Bsp19I CCATGG 2 cut(s) 990, 1530
BspCNI CTCAG 5 cut(s) 147, 298, 1139, 1323, 1503
BspFNI CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 6 cut(s) 184, 682, 1259, 1272, 1683, 1772
BspPI GGATC 3 cut(s) 266, 384, 557
BspQI GCTCTTC 1 cut(s) 1607
BspT107I GGYRCC 4 cut(s) 182, 680, 1270, 1681
BspTNI GGTCTC 2 cut(s) 143, 704
BsrBI CCGCTC 2 cut(s) 814, 1617
BsrFI RCCGGY 2 cut(s) 40, 1557
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 8 cut(s) 218, 410, 737, 906, 949, 1466, 1672, 1725
BssAI RCCGGY 2 cut(s) 40, 1557
BssECI CCNNGG 9 cut(s) 60, 442, 676, 990, 1441, 1530, 1685, 1728, 1773
BssMI GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
BssNI GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
BssT1I CCWWGG 4 cut(s) 60, 442, 990, 1530
Bst2UI CCWGG 3 cut(s) 110, 432, 1729
Bst4CI ACNGT 3 cut(s) 118, 194, 1788
Bst6I CTCTTC 1 cut(s) 1607
BstACI GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 1 cut(s) 1604
BstC8I GCNNGC 5 cut(s) 123, 539, 997, 1353, 1399
BstDEI CTNAG 5 cut(s) 134, 306, 1126, 1310, 1511
BstDSI CCRYGG 3 cut(s) 990, 1530, 1685
BstEII GGTNACC 1 cut(s) 1249
BstF5I GGATG 6 cut(s) 295, 597, 711, 753, 809, 1437
BstFNI CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
BstH2I RGCGCY 4 cut(s) 186, 661, 684, 1274
BstHHI GCGC 6 cut(s) 185, 660, 683, 710, 1273, 1661
BstKTI GATC 6 cut(s) 261, 274, 379, 565, 1587, 1650
BstMAI GTCTC 4 cut(s) 107, 143, 704, 1777
BstMBI GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
BstMCI CGRYCG 2 cut(s) 1488, 1587
BstMWI GCNNNNNNNGC 2 cut(s) 689, 733
BstNI CCWGG 3 cut(s) 110, 432, 1729
BstPI GGTNACC 1 cut(s) 1249
BstSFI CTRYAG 1 cut(s) 973
BstUI CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
BstV1I GCAGC 2 cut(s) 748, 1508
BstXI CCANNNNNNTGG 1 cut(s) 1405
BstZI CGGCCG 1 cut(s) 1485
BtgI CCRYGG 3 cut(s) 990, 1530, 1685
BtrI CACGTC 1 cut(s) 1168
BtsCI GGATG 6 cut(s) 295, 597, 711, 753, 809, 1437
BtsI GCAGTG 1 cut(s) 1568
BtsIMutI CAGTG 4 cut(s) 186, 1134, 1523, 1568
Cac8I GCNNGC 5 cut(s) 123, 539, 997, 1353, 1399
CciI TCATGA 1 cut(s) 414
CfoI GCGC 6 cut(s) 185, 660, 683, 710, 1273, 1661
Cfr10I RCCGGY 2 cut(s) 40, 1557
Cfr13I GGNCC 8 cut(s) 35, 439, 505, 903, 946, 1444, 1589, 1770
Cfr42I CCGCGG 1 cut(s) 1688
Cfr9I CCCGGG 1 cut(s) 1441
CseI GACGC 5 cut(s) 65, 110, 174, 1219, 1650
Csp6I GTAC 6 cut(s) 327, 559, 628, 909, 1006, 1411
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 9 cut(s) 406, 415, 625, 808, 991, 1003, 1090, 1159, 1531
CviQI GTAC 6 cut(s) 327, 559, 628, 909, 1006, 1411
DdeI CTNAG 5 cut(s) 134, 306, 1126, 1310, 1511
DinI GGCGCC 3 cut(s) 184, 682, 1272
DpnI GATC 6 cut(s) 260, 273, 378, 564, 1586, 1649
DpnII GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
DraI TTTAAA 1 cut(s) 205
DraIII CACNNNGTG 1 cut(s) 1288
DrdI GACNNNNNNGTC 2 cut(s) 929, 1172
DseDI GACNNNNNNGTC 2 cut(s) 929, 1172
EaeI YGGCCR 2 cut(s) 1485, 1527
EagI CGGCCG 1 cut(s) 1485
Eam1104I CTCTTC 1 cut(s) 1607
EarI CTCTTC 1 cut(s) 1607
EciI GGCGGA 5 cut(s) 510, 662, 1309, 1355, 1589
Ecl136II GAGCTC 2 cut(s) 1192, 1345
EclXI CGGCCG 1 cut(s) 1485
Eco130I CCWWGG 4 cut(s) 60, 442, 990, 1530
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 3 cut(s) 1122, 1194, 1347
Eco31I GGTCTC 2 cut(s) 143, 704
Eco47I GGWCC 2 cut(s) 439, 1770
Eco52I CGGCCG 1 cut(s) 1485
Eco53kI GAGCTC 2 cut(s) 1192, 1345
Eco57I CTGAAG 2 cut(s) 315, 1044
Eco88I CYCGRG 5 cut(s) 32, 653, 827, 1441, 1651
Eco91I GGTNACC 1 cut(s) 1249
EcoICRI GAGCTC 2 cut(s) 1192, 1345
EcoO65I GGTNACC 1 cut(s) 1249
EcoRII CCWGG 3 cut(s) 108, 430, 1727
EcoT14I CCWWGG 4 cut(s) 60, 442, 990, 1530
EcoT22I ATGCAT 1 cut(s) 809
EcoT38I GRGCYC 3 cut(s) 1122, 1194, 1347
EgeI GGCGCC 3 cut(s) 184, 682, 1272
EheI GGCGCC 3 cut(s) 184, 682, 1272
ErhI CCWWGG 4 cut(s) 60, 442, 990, 1530
Esp3I CGTCTC 2 cut(s) 107, 1777
FaeI CATG 9 cut(s) 409, 418, 628, 811, 994, 1006, 1093, 1162, 1534
FaqI GGGAC 1 cut(s) 881
FatI CATG 9 cut(s) 405, 414, 624, 807, 990, 1002, 1089, 1158, 1530
FauI CCCGC 2 cut(s) 328, 857
FblI GTMKAC 3 cut(s) 576, 1164, 1359
Fnu4HI GCNGC 9 cut(s) 737, 747, 979, 982, 1274, 1292, 1522, 1618, 1685
FokI GGATG 6 cut(s) 282, 584, 698, 740, 816, 1424
FriOI GRGCYC 3 cut(s) 1122, 1194, 1347
Fsp4HI GCNGC 9 cut(s) 737, 747, 979, 982, 1274, 1292, 1522, 1618, 1685
FspBI CTAG 1 cut(s) 1472
GlaI GCGC 6 cut(s) 184, 659, 682, 709, 1272, 1660
GluI GCNGC 9 cut(s) 737, 747, 979, 982, 1274, 1292, 1522, 1618, 1685
GsuI CTGGAG 4 cut(s) 224, 707, 1331, 1711
HaeII RGCGCY 4 cut(s) 186, 661, 684, 1274
HgaI GACGC 5 cut(s) 65, 110, 174, 1219, 1650
HhaI GCGC 6 cut(s) 185, 660, 683, 710, 1273, 1661
Hin1I GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
Hin1II CATG 9 cut(s) 409, 418, 628, 811, 994, 1006, 1093, 1162, 1534
Hin6I GCGC 6 cut(s) 183, 658, 681, 708, 1271, 1659
HinP1I GCGC 6 cut(s) 183, 658, 681, 708, 1271, 1659
HincII GTYRAC 4 cut(s) 10, 1156, 1165, 1360
HindII GTYRAC 4 cut(s) 10, 1156, 1165, 1360
HphI GGTGA 5 cut(s) 307, 350, 925, 1243, 1621
Hpy166II GTNNAC 8 cut(s) 10, 577, 630, 1156, 1165, 1360, 1413, 1555
Hpy188III TCNNGA 7 cut(s) 365, 415, 547, 920, 1136, 1651, 1653
Hpy8I GTNNAC 8 cut(s) 10, 577, 630, 1156, 1165, 1360, 1413, 1555
Hpy99I CGWCG 9 cut(s) 104, 168, 384, 926, 1169, 1235, 1337, 1361, 1666
HpyAV CCTTC 5 cut(s) 29, 33, 410, 1068, 1764
HpyCH4III ACNGT 3 cut(s) 118, 194, 1788
HpyCH4IV ACGT 6 cut(s) 924, 1167, 1302, 1452, 1603, 1622
HpyCH4V TGCA 4 cut(s) 70, 807, 999, 1053
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 733
HpyF3I CTNAG 5 cut(s) 134, 306, 1126, 1310, 1511
HpySE526I ACGT 6 cut(s) 924, 1167, 1302, 1452, 1603, 1622
Hsp92I GRCGYC 6 cut(s) 57, 102, 183, 681, 924, 1271
Hsp92II CATG 9 cut(s) 409, 418, 628, 811, 994, 1006, 1093, 1162, 1534
HspAI GCGC 6 cut(s) 183, 658, 681, 708, 1271, 1659
KasI GGCGCC 3 cut(s) 182, 680, 1270
KspI CCGCGG 1 cut(s) 1688
Kzo9I GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
LguI GCTCTTC 1 cut(s) 1607
LmnI GCTCC 6 cut(s) 281, 811, 938, 1342, 1350, 1465
Lsp1109I GCAGC 2 cut(s) 748, 1508
LweI GCATC 2 cut(s) 342, 794
MaeI CTAG 1 cut(s) 1472
MaeII ACGT 6 cut(s) 924, 1167, 1302, 1452, 1603, 1622
MaeIII GTNAC 4 cut(s) 931, 1022, 1249, 1716
MalI GATC 6 cut(s) 260, 273, 378, 564, 1586, 1649
MbiI CCGCTC 2 cut(s) 814, 1617
MboI GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
MboII GAAGA 3 cut(s) 206, 1309, 1624
MhlI GDGCHC 4 cut(s) 123, 1122, 1194, 1347
MlsI TGGCCA 1 cut(s) 1529
MluCI AATT 6 cut(s) 71, 780, 874, 897, 1077, 1696
MluNI TGGCCA 1 cut(s) 1529
Mly113I GGCGCC 3 cut(s) 183, 681, 1271
MmeI TCCRAC 5 cut(s) 187, 983, 1156, 1347, 1575
Mox20I TGGCCA 1 cut(s) 1529
Mph1103I ATGCAT 1 cut(s) 809
MscI TGGCCA 1 cut(s) 1529
MseI TTAA 2 cut(s) 204, 1668
MslI CAYNNNNRTG 2 cut(s) 635, 1079
Msp20I TGGCCA 1 cut(s) 1529
MspA1I CMGCKG 3 cut(s) 335, 978, 1687
MvaI CCWGG 3 cut(s) 110, 432, 1729
MvnI CGCG 5 cut(s) 708, 1230, 1646, 1661, 1687
MwoI GCNNNNNNNGC 2 cut(s) 689, 733
NarI GGCGCC 3 cut(s) 183, 681, 1271
NciI CCSGG 8 cut(s) 492, 791, 1247, 1255, 1442, 1443, 1568, 1775
NcoI CCATGG 2 cut(s) 990, 1530
NdeII GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
NlaIII CATG 9 cut(s) 409, 418, 628, 811, 994, 1006, 1093, 1162, 1534
NlaIV GGNNCC 6 cut(s) 184, 682, 1259, 1272, 1683, 1772
NmeAIII GCCGAG 7 cut(s) 151, 172, 269, 657, 1247, 1463, 1513
NmuCI GTSAC 2 cut(s) 931, 1249
NsiI ATGCAT 1 cut(s) 809
PaeR7I CTCGAG 2 cut(s) 653, 1651
PagI TCATGA 1 cut(s) 414
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 1607
PcsI WCGNNNNNNNCGW 7 cut(s) 102, 150, 474, 927, 1014, 1589, 1658
PfeI GAWTC 7 cut(s) 130, 235, 361, 479, 885, 1505, 1538
Pfl23II CGTACG 1 cut(s) 558
PflFI GACNNNGTC 2 cut(s) 104, 1160
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 9 cut(s) 738, 748, 980, 983, 1275, 1293, 1523, 1619, 1686
Ple19I CGATCG 1 cut(s) 1587
PluTI GGCGCC 3 cut(s) 186, 684, 1274
Ppu21I YACGTR 1 cut(s) 1604
Psp124BI GAGCTC 2 cut(s) 1194, 1347
Psp6I CCWGG 3 cut(s) 108, 430, 1727
PspEI GGTNACC 1 cut(s) 1249
PspGI CCWGG 3 cut(s) 108, 430, 1727
PspLI CGTACG 1 cut(s) 558
PspN4I GGNNCC 6 cut(s) 184, 682, 1259, 1272, 1683, 1772
PspPI GGNCC 8 cut(s) 35, 439, 505, 903, 946, 1444, 1589, 1770
PspXI VCTCGAGB 1 cut(s) 653
PsyI GACNNNGTC 2 cut(s) 104, 1160
PvuI CGATCG 1 cut(s) 1587
RsaI GTAC 6 cut(s) 328, 560, 629, 910, 1007, 1412
RsaNI GTAC 6 cut(s) 327, 559, 628, 909, 1006, 1411
RseI CAYNNNNRTG 2 cut(s) 635, 1079
SacI GAGCTC 2 cut(s) 1194, 1347
SacII CCGCGG 1 cut(s) 1688
SalI GTCGAC 2 cut(s) 1163, 1358
SapI GCTCTTC 1 cut(s) 1607
SaqAI TTAA 2 cut(s) 204, 1668
SatI GCNGC 9 cut(s) 737, 747, 979, 982, 1274, 1292, 1522, 1618, 1685
Sau3AI GATC 6 cut(s) 258, 271, 376, 562, 1584, 1647
Sau96I GGNCC 8 cut(s) 35, 439, 505, 903, 946, 1444, 1589, 1770
SduI GDGCHC 4 cut(s) 123, 1122, 1194, 1347
SfaNI GCATC 2 cut(s) 342, 794
SfcI CTRYAG 1 cut(s) 973
SfoI GGCGCC 3 cut(s) 184, 682, 1272
Sfr274I CTCGAG 2 cut(s) 653, 1651
Sfr303I CCGCGG 1 cut(s) 1688
SgrBI CCGCGG 1 cut(s) 1688
SinI GGWCC 2 cut(s) 439, 1770
SlaI CTCGAG 2 cut(s) 653, 1651
SmaI CCCGGG 1 cut(s) 1443
SmiMI CAYNNNNRTG 2 cut(s) 635, 1079
SmlI CTYRAG 3 cut(s) 653, 1382, 1651
SmoI CTYRAG 3 cut(s) 653, 1382, 1651
Sse9I AATT 6 cut(s) 71, 780, 874, 897, 1077, 1696
SseBI AGGCCT 1 cut(s) 65
SspDI GGCGCC 3 cut(s) 182, 680, 1270
SspMI CTAG 1 cut(s) 1472
SstI GAGCTC 2 cut(s) 1194, 1347
StuI AGGCCT 1 cut(s) 65
StyI CCWWGG 4 cut(s) 60, 442, 990, 1530
TaaI ACNGT 3 cut(s) 118, 194, 1788
TaiI ACGT 6 cut(s) 927, 1170, 1305, 1455, 1606, 1625
TaqII GACCGA 2 cut(s) 167, 1346
TasI AATT 6 cut(s) 71, 780, 874, 897, 1077, 1696
TatI WGTACW 3 cut(s) 627, 908, 1410
TauI GCSGC 7 cut(s) 749, 981, 984, 1276, 1294, 1620, 1687
TfiI GAWTC 7 cut(s) 130, 235, 361, 479, 885, 1505, 1538
Tru1I TTAA 2 cut(s) 204, 1668
Tru9I TTAA 2 cut(s) 204, 1668
TscAI CASTG 4 cut(s) 193, 1134, 1523, 1568
TseFI GTSAC 2 cut(s) 931, 1249
TseI GCWGC 2 cut(s) 736, 1521
Tsp45I GTSAC 2 cut(s) 931, 1249
TspDTI ATGAA 3 cut(s) 64, 431, 1059
TspMI CCCGGG 1 cut(s) 1441
TspRI CASTG 4 cut(s) 193, 1134, 1523, 1568
Tth111I GACNNNGTC 2 cut(s) 104, 1160
VpaK11BI GGWCC 2 cut(s) 439, 1770
XapI RAATTY 2 cut(s) 897, 1696
XhoI CTCGAG 2 cut(s) 653, 1651
XmaI CCCGGG 1 cut(s) 1441
XmiI GTMKAC 3 cut(s) 576, 1164, 1359
XspI CTAG 1 cut(s) 1472
ZraI GACGTC 1 cut(s) 925
Zsp2I ATGCAT 1 cut(s) 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.