MD08G1080000.v1.1

Uncharacterised conserved protein UCP031279 (InterPro IPR016972)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000667
Physical Location & Seq
Forward (+)
6658815 .. 6659345
531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1080000.v1.1.491

Sequence Viewer

Length: 531 bp
ATGAGGAAGACCAAGGTGAATAGCCAGAACAAGTTCATGAAAATCATCATGGTACCCATCAGAGTCCTGAGCAAGGCCAAAGACTTTTACGTCAGAAGCATGACGGACATGGGCGGCAGAGTGGCCTACAGTGGCGCAGTTGGAGGGCTTGGTGGTAATGTCACTCAAGGCTTGCCCAAGAGCTTCAGCGTTAGCTCAACTTCTTCTAGGTTAAGTGAAAATGGTGATGATTATTCAGAGCTTATTAGGGCTGCGTCAGCAAGGAACTACGGGGACAGGATCGACGTGAACATGATCCTGCAAGAGCAGATGAAACGATCAGCTACAACCACAACTATGCGATCGGGTATGGGACCGAAAAGGGTATTTAATAATCCGACTGTTTTGCCAAAATGTGGCAGCGTGGCCATGGGGAAGATTGATGAAGATGCACCTGCGGATTTTGATGAAGGTGCTGCTGGTGTCAAGGGTGACTTGTACCCTAGAAGCAAAAGCTATGCTGTTGGAAGGAGAAGTGCTTTTGCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.05

Weight (kDa)

9.95

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 442
AasI GACNNNNNNGTC 1 cut(s) 89
Acc36I ACCTGC 1 cut(s) 442
Acc65I GGTACC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 52
AccB7I CCANNNNNTGG 1 cut(s) 395
AciI CCGC 2 cut(s) 114, 437
AclWI GGATC 2 cut(s) 287, 289
AcoI YGGCCR 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 169
AfaI GTAC 2 cut(s) 54, 479
AfiI CCNNNNNNNGG 2 cut(s) 73, 395
AjiI CACGTC 1 cut(s) 286
AluBI AGCT 5 cut(s) 183, 195, 241, 323, 495
AluI AGCT 5 cut(s) 183, 195, 241, 323, 495
AlwI GGATC 2 cut(s) 287, 289
AoxI GGCC 3 cut(s) 75, 123, 405
ApeKI GCWGC 3 cut(s) 251, 399, 455
Asp700I GAANNNNTTC 1 cut(s) 32
Asp718I GGTACC 1 cut(s) 52
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 3 cut(s) 28, 236, 482
AvaII GGWCC 1 cut(s) 353
BalI TGGCCA 1 cut(s) 407
BanI GGYRCC 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 14
BbvI GCAGC 3 cut(s) 238, 411, 442
BccI CCATC 1 cut(s) 65
BcgI CGANNNNNNTGC 2 cut(s) 367, 401
BfaI CTAG 2 cut(s) 207, 483
BfmI CTRYAG 1 cut(s) 127
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 4 cut(s) 115, 252, 400, 456
BlsI GCNGC 4 cut(s) 116, 253, 401, 457
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 54, 354
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 14
Bpu10I CCTNAGC 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 150
BsaJI CCNNGG 2 cut(s) 12, 408
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 395
BseDI CCNNGG 2 cut(s) 12, 408
BseLI CCNNNNNNNGG 2 cut(s) 73, 395
BseMII CTCAG 1 cut(s) 59
BseXI GCAGC 3 cut(s) 238, 411, 442
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 77, 125, 407
BshNI GGYRCC 1 cut(s) 52
BsiEI CGRYCG 1 cut(s) 344
BslFI GGGAC 2 cut(s) 287, 366
BslI CCNNNNNNNGG 2 cut(s) 73, 395
BsmFI GGGAC 2 cut(s) 287, 366
BsnI GGCC 3 cut(s) 77, 125, 407
Bsp143I GATC 4 cut(s) 279, 294, 317, 341
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 2 cut(s) 114, 437
BspANI GGCC 3 cut(s) 77, 125, 407
BspCNI CTCAG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 36
BspLI GGNNCC 2 cut(s) 54, 354
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 2 cut(s) 287, 289
BspT107I GGYRCC 1 cut(s) 52
BssECI CCNNGG 2 cut(s) 12, 408
BssMI GATC 4 cut(s) 279, 294, 317, 341
BssT1I CCWWGG 2 cut(s) 12, 408
Bst4CI ACNGT 2 cut(s) 131, 382
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 408
BstENI CCTNNNNNAGG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 4 cut(s) 282, 297, 320, 344
BstMBI GATC 4 cut(s) 279, 294, 317, 341
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstSFI CTRYAG 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 238, 411, 442
BstV2I GAAGAC 1 cut(s) 14
BsuRI GGCC 3 cut(s) 77, 125, 407
BtgI CCRYGG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 136
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 173
CciI TCATGA 1 cut(s) 36
CfoI GCGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 353
CseI GACGC 1 cut(s) 243
Csp6I GTAC 2 cut(s) 53, 478
CviAII CATG 6 cut(s) 37, 49, 100, 109, 292, 409
CviQI GTAC 2 cut(s) 53, 478
DdeI CTNAG 1 cut(s) 68
DpnI GATC 4 cut(s) 281, 296, 319, 343
DpnII GATC 4 cut(s) 279, 294, 317, 341
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 12, 408
Eco47I GGWCC 1 cut(s) 353
Eco57I CTGAAG 1 cut(s) 169
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 12, 408
ErhI CCWWGG 2 cut(s) 12, 408
FaeI CATG 6 cut(s) 40, 52, 103, 112, 295, 412
FaiI YATR 9 cut(s) 38, 50, 101, 110, 293, 338, 350, 410, 498
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 287, 366
FatI CATG 6 cut(s) 36, 48, 99, 108, 291, 408
Fnu4HI GCNGC 4 cut(s) 115, 252, 400, 456
Fsp4HI GCNGC 4 cut(s) 115, 252, 400, 456
FspBI CTAG 2 cut(s) 207, 483
GlaI GCGC 1 cut(s) 136
GluI GCNGC 4 cut(s) 115, 252, 400, 456
HaeIII GGCC 3 cut(s) 77, 125, 407
HgaI GACGC 1 cut(s) 243
HhaI GCGC 1 cut(s) 137
Hin1II CATG 6 cut(s) 40, 52, 103, 112, 295, 412
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HinfI GANTC 1 cut(s) 63
HphI GGTGA 3 cut(s) 28, 236, 482
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 4 cut(s) 62, 95, 238, 378
Hpy188III TCNNGA 2 cut(s) 37, 67
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 2 cut(s) 443, 501
HpyCH4III ACNGT 2 cut(s) 131, 382
HpyCH4IV ACGT 2 cut(s) 90, 285
HpyCH4V TGCA 2 cut(s) 301, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 2 cut(s) 90, 285
Hsp92II CATG 6 cut(s) 40, 52, 103, 112, 295, 412
HspAI GCGC 1 cut(s) 135
KpnI GGTACC 1 cut(s) 56
Kzo9I GATC 4 cut(s) 279, 294, 317, 341
LpnPI CCDG 6 cut(s) 38, 80, 262, 311, 444, 447
Lsp1109I GCAGC 3 cut(s) 238, 411, 442
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 207, 483
MaeII ACGT 2 cut(s) 90, 285
MaeIII GTNAC 2 cut(s) 160, 470
MalI GATC 4 cut(s) 281, 296, 319, 343
MboI GATC 4 cut(s) 279, 294, 317, 341
MboII GAAGA 4 cut(s) 19, 195, 427, 437
MlsI TGGCCA 1 cut(s) 407
MluNI TGGCCA 1 cut(s) 407
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 3 cut(s) 121, 401, 484
MnlI CCTC 1 cut(s) 137
Mox20I TGGCCA 1 cut(s) 407
MroXI GAANNNNTTC 1 cut(s) 32
MscI TGGCCA 1 cut(s) 407
MseI TTAA 2 cut(s) 212, 369
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 1 cut(s) 407
MwoI GCNNNNNNNGC 1 cut(s) 257
NcoI CCATGG 1 cut(s) 408
NdeII GATC 4 cut(s) 279, 294, 317, 341
NlaIII CATG 6 cut(s) 40, 52, 103, 112, 295, 412
NlaIV GGNNCC 2 cut(s) 54, 354
NmuCI GTSAC 2 cut(s) 160, 470
PagI TCATGA 1 cut(s) 36
PaqCI CACCTGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 32
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 4 cut(s) 116, 253, 401, 457
Ple19I CGATCG 1 cut(s) 344
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 54, 354
PspPI GGNCC 1 cut(s) 353
PvuI CGATCG 1 cut(s) 344
RsaI GTAC 2 cut(s) 54, 479
RsaNI GTAC 2 cut(s) 53, 478
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 2 cut(s) 212, 369
SatI GCNGC 4 cut(s) 115, 252, 400, 456
Sau3AI GATC 4 cut(s) 279, 294, 317, 341
Sau96I GGNCC 1 cut(s) 353
SchI GAGTC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 1 cut(s) 127
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 1 cut(s) 165
SmoI CTYRAG 1 cut(s) 165
SsiI CCGC 2 cut(s) 114, 437
SspMI CTAG 2 cut(s) 207, 483
StyI CCWWGG 2 cut(s) 12, 408
TaaI ACNGT 2 cut(s) 131, 382
TaiI ACGT 2 cut(s) 93, 288
TaqI TCGA 1 cut(s) 282
TaqII GACCGA 1 cut(s) 370
TauI GCSGC 1 cut(s) 117
Tru1I TTAA 2 cut(s) 212, 369
Tru9I TTAA 2 cut(s) 212, 369
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 2 cut(s) 160, 470
TseI GCWGC 3 cut(s) 251, 399, 455
Tsp45I GTSAC 2 cut(s) 160, 470
TspDTI ATGAA 5 cut(s) 25, 53, 326, 438, 462
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 1 cut(s) 136
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 353
XagI CCTNNNNNAGG 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 2 cut(s) 207, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.