pycom15g26190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000667
Physical Location & Seq
Reverse (-)
20901140 .. 20901628
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g26190.1

Sequence Viewer

Length: 489 bp
ATGGCAAACATGAAGAAACCTACCAAAGAAAGCAGACTCACAAAGTTCATGAAGGCCCCCCTAAGGGTTTTAATCAAGGCCAGGGATTTCTACATCATGAGCATGACCGAGTGCTCCGGTCGATTCGATTACGGCACGGTCATGGGATGCCCTGCTGCCCAAGTCCCAAATGCTTTGTCTAGAAGCTTCAGCACAAGCTCTACCAAATCAAGCACTGCCGATAACGAAGATTACAGGGAGCTTATGAGGGCTGCTTCTGATAGGAGCGTAAGGAATAAAATTGAATTCGATCTGGCGCGAAAAGCTCAGCTCCCTAGGCAGCCTCCGGTGGCAGGAGTGCCCAACGCCATGCCTCGCAGTCGAAGTGTTGGAATCGGAAGGATCGACGAAGACAAGATGTGTGAATTTGATGAAGAAGAAGAAGTCAAGATCAAGAGAGAAGTGTATCTGAGGAGTAGGAGCTATGCTGTGTCTAGGAGAACAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.44

Weight (kDa)

9.77

Isoelectric Point (pI)

75.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 298
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 2 cut(s) 284, 404
AcuI CTGAAG 1 cut(s) 172
AfiI CCNNNNNNNGG 3 cut(s) 63, 64, 332
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 6 cut(s) 186, 198, 241, 305, 310, 462
AluI AGCT 6 cut(s) 186, 198, 241, 305, 310, 462
Alw21I GWGCWC 1 cut(s) 116
AlwI GGATC 1 cut(s) 389
AoxI GGCC 2 cut(s) 54, 78
ApeKI GCWGC 3 cut(s) 155, 251, 319
ApoI RAATTY 2 cut(s) 284, 404
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspA2I CCTAGG 1 cut(s) 314
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 1 cut(s) 55
AvrII CCTAGG 1 cut(s) 314
AxyI CCTNAGG 1 cut(s) 62
BaeGI GKGCMC 1 cut(s) 342
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 116
BbvI GCAGC 3 cut(s) 142, 238, 331
BceAI ACGGC 1 cut(s) 148
BciT130I CCWGG 1 cut(s) 82
BfaI CTAG 3 cut(s) 180, 315, 474
BisI GCNGC 3 cut(s) 156, 252, 320
BlnI CCTAGG 1 cut(s) 314
BlpI GCTNAGC 1 cut(s) 306
BlsI GCNGC 3 cut(s) 157, 253, 321
Bme1390I CCNGG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 306
BsaJI CCNNGG 2 cut(s) 81, 314
BsaWI WCCGGW 2 cut(s) 116, 325
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 64, 332
Bse21I CCTNAGG 1 cut(s) 62
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 2 cut(s) 81, 314
BseGI GGATG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 63, 64, 332
BseMII CTCAG 2 cut(s) 320, 440
BseRI GAGGAG 1 cut(s) 466
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 3 cut(s) 142, 238, 331
Bsh1236I CGCG 1 cut(s) 298
Bsh1285I CGRYCG 1 cut(s) 121
BshFI GGCC 2 cut(s) 56, 80
BsiEI CGRYCG 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 116
BsiSI CCGG 2 cut(s) 117, 326
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 63, 64, 332
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 2 cut(s) 56, 80
Bsp1286I GDGCHC 2 cut(s) 116, 342
Bsp143I GATC 3 cut(s) 289, 381, 429
Bsp1720I GCTNAGC 1 cut(s) 306
BspANI GGCC 2 cut(s) 56, 80
BspCNI CTCAG 2 cut(s) 319, 441
BspFNI CGCG 1 cut(s) 298
BspHI TCATGA 2 cut(s) 48, 96
BspLI GGNNCC 1 cut(s) 57
BspPI GGATC 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 81, 314
BssMI GATC 3 cut(s) 289, 381, 429
BssT1I CCWWGG 1 cut(s) 314
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 139
BstDEI CTNAG 3 cut(s) 62, 306, 449
BstF5I GGATG 1 cut(s) 152
BstFNI CGCG 1 cut(s) 298
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 3 cut(s) 292, 384, 432
BstMBI GATC 3 cut(s) 289, 381, 429
BstMCI CGRYCG 1 cut(s) 121
BstMWI GCNNNNNNNGC 2 cut(s) 302, 316
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstSLI GKGCMC 1 cut(s) 342
BstUI CGCG 1 cut(s) 298
BstV1I GCAGC 3 cut(s) 142, 238, 331
BstV2I GAAGAC 1 cut(s) 396
Bsu36I CCTNAGG 1 cut(s) 62
BsuRI GGCC 2 cut(s) 56, 80
BtsCI GGATG 1 cut(s) 152
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 1 cut(s) 213
CciI TCATGA 2 cut(s) 48, 96
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 55
CviAII CATG 6 cut(s) 10, 49, 97, 103, 142, 349
DdeI CTNAG 3 cut(s) 62, 306, 449
DpnI GATC 3 cut(s) 291, 383, 431
DpnII GATC 3 cut(s) 289, 381, 429
Eco130I CCWWGG 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 55
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 6 cut(s) 13, 52, 100, 106, 145, 352
FaiI YATR 8 cut(s) 11, 50, 98, 104, 143, 245, 350, 465
FaqI GGGAC 1 cut(s) 149
FatI CATG 6 cut(s) 9, 48, 96, 102, 141, 348
Fnu4HI GCNGC 3 cut(s) 156, 252, 320
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 3 cut(s) 156, 252, 320
FspBI CTAG 3 cut(s) 180, 315, 474
GlaI GCGC 1 cut(s) 297
GluI GCNGC 3 cut(s) 156, 252, 320
HaeIII GGCC 2 cut(s) 56, 80
HapII CCGG 2 cut(s) 117, 326
HhaI GCGC 1 cut(s) 298
Hin1II CATG 6 cut(s) 13, 52, 100, 106, 145, 352
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 184
HinfI GANTC 3 cut(s) 36, 123, 372
HpaII CCGG 2 cut(s) 117, 326
Hpy188I TCNGA 3 cut(s) 259, 377, 450
Hpy188III TCNNGA 5 cut(s) 49, 97, 180, 427, 433
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 46, 372
HpyCH4III ACNGT 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 316
HpyF3I CTNAG 3 cut(s) 62, 306, 449
Hsp92II CATG 6 cut(s) 13, 52, 100, 106, 145, 352
HspAI GCGC 1 cut(s) 296
Kzo9I GATC 3 cut(s) 289, 381, 429
LmnI GCTCC 5 cut(s) 119, 238, 264, 315, 459
LpnPI CCDG 8 cut(s) 67, 94, 130, 165, 220, 278, 318, 339
Lsp1109I GCAGC 3 cut(s) 142, 238, 331
LweI GCATC 1 cut(s) 137
MaeI CTAG 3 cut(s) 180, 315, 474
MalI GATC 3 cut(s) 291, 383, 431
MboI GATC 3 cut(s) 289, 381, 429
MboII GAAGA 6 cut(s) 25, 239, 401, 425, 428, 431
MhlI GDGCHC 2 cut(s) 116, 342
MluCI AATT 4 cut(s) 279, 284, 404, 484
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 4 cut(s) 240, 333, 363, 444
MseI TTAA 1 cut(s) 71
MslI CAYNNNNRTG 2 cut(s) 101, 140
MspI CCGG 2 cut(s) 117, 326
MspR9I CCNGG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 1 cut(s) 298
MwoI GCNNNNNNNGC 2 cut(s) 302, 316
NdeII GATC 3 cut(s) 289, 381, 429
NlaIII CATG 6 cut(s) 13, 52, 100, 106, 145, 352
NlaIV GGNNCC 1 cut(s) 57
PagI TCATGA 2 cut(s) 48, 96
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 2 cut(s) 123, 372
PkrI GCNGC 3 cut(s) 157, 253, 321
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 57
PspPI GGNCC 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 101, 140
SaqAI TTAA 1 cut(s) 71
SatI GCNGC 3 cut(s) 156, 252, 320
Sau3AI GATC 3 cut(s) 289, 381, 429
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 2 cut(s) 116, 342
SetI ASST 7 cut(s) 22, 188, 200, 243, 307, 312, 464
SfaNI GCATC 1 cut(s) 137
SmiMI CAYNNNNRTG 2 cut(s) 101, 140
Sse9I AATT 4 cut(s) 279, 284, 404, 484
SspMI CTAG 3 cut(s) 180, 315, 474
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 1 cut(s) 139
TaqI TCGA 5 cut(s) 121, 126, 288, 361, 384
TaqII GACCGA 1 cut(s) 122
TasI AATT 4 cut(s) 279, 284, 404, 484
TfiI GAWTC 2 cut(s) 123, 372
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 1 cut(s) 220
TseI GCWGC 3 cut(s) 155, 251, 319
TspDTI ATGAA 4 cut(s) 26, 37, 65, 426
TspRI CASTG 1 cut(s) 220
XapI RAATTY 2 cut(s) 284, 404
XbaI TCTAGA 1 cut(s) 179
XmaJI CCTAGG 1 cut(s) 314
XspI CTAG 3 cut(s) 180, 315, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.