MD15G1299400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000667
Physical Location & Seq
Reverse (-)
28231139 .. 28231618
480 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1299400.v1.1.491

Sequence Viewer

Length: 480 bp
ATGAAGAAACCTACCAAAGAAAGCAGACTCACAAAGTTCATGAAGGCCCCCCTAAGGATTTTAATCAAGGCCAGGGATTTCTACATCATGAGCATGACCGAGTGCTCCGGTCGATTCGATTACGGCACGGTCATGGGATGCCCTGCTGCCCAAGTCCCAAGTGCTTTGCCTAGAAGCTTCAGCACAAGCTCTACCAAATCAAGCACTGCCGATAACGAAGATTACAGGGAGCTTATGAGGGCTGCTTCTGCTAGGAGCGTAAGGAATAAAATTGAATTCGATCTGGCGCGAAAAGTTCAGCTCCCTAGGCAGCCTCCGGTGGCGGGAGTGCCCAACGCCATACCTCGCAGTCGAAGTGTTGGAATCAGAAGGATCGACGAAGACAAGATGTGTGAATTTGATGAAGAAGAAGAAGTCAAGATCAAGAGAGCGGTGTATCCGAGGAGTAGGAGCTATGCTGTTTCTAGGAGAACAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.11

Weight (kDa)

10.05

Isoelectric Point (pI)

77.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 431
AccII CGCG 1 cut(s) 289
AciI CCGC 2 cut(s) 323, 431
AclWI GGATC 1 cut(s) 380
AcsI RAATTY 2 cut(s) 275, 395
AcuI CTGAAG 1 cut(s) 163
AfiI CCNNNNNNNGG 2 cut(s) 54, 323
AgsI TTSAA 1 cut(s) 275
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 5 cut(s) 177, 189, 232, 301, 453
AluI AGCT 5 cut(s) 177, 189, 232, 301, 453
Alw21I GWGCWC 1 cut(s) 107
AlwI GGATC 1 cut(s) 380
AoxI GGCC 2 cut(s) 45, 69
ApeKI GCWGC 3 cut(s) 146, 242, 310
ApoI RAATTY 2 cut(s) 275, 395
AspA2I CCTAGG 1 cut(s) 305
AspLEI GCGC 1 cut(s) 289
AspS9I GGNCC 1 cut(s) 46
AvrII CCTAGG 1 cut(s) 305
AxyI CCTNAGG 1 cut(s) 53
BaeGI GKGCMC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 3 cut(s) 133, 229, 322
BceAI ACGGC 1 cut(s) 139
BciT130I CCWGG 1 cut(s) 73
BciVI GTATCC 1 cut(s) 447
BfaI CTAG 4 cut(s) 171, 252, 306, 465
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 3 cut(s) 147, 243, 311
BlnI CCTAGG 1 cut(s) 305
BlsI GCNGC 3 cut(s) 148, 244, 312
Bme1390I CCNGG 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 73
BmsI GCATC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 387
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 72, 305, 440
BsaWI WCCGGW 2 cut(s) 107, 316
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 323
Bse21I CCTNAGG 1 cut(s) 53
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 3 cut(s) 72, 305, 440
BseGI GGATG 1 cut(s) 143
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 54, 323
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 1 cut(s) 333
BseXI GCAGC 3 cut(s) 133, 229, 322
Bsh1236I CGCG 1 cut(s) 289
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 47, 71
BsiEI CGRYCG 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 107
BsiSI CCGG 2 cut(s) 108, 317
BslFI GGGAC 1 cut(s) 140
BslI CCNNNNNNNGG 2 cut(s) 54, 323
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 47, 71
Bsp1286I GDGCHC 2 cut(s) 107, 333
Bsp143I GATC 3 cut(s) 280, 372, 420
BspACI CCGC 2 cut(s) 323, 431
BspANI GGCC 2 cut(s) 47, 71
BspFNI CGCG 1 cut(s) 289
BspHI TCATGA 2 cut(s) 39, 87
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 1 cut(s) 380
BsrBI CCGCTC 1 cut(s) 431
BssECI CCNNGG 3 cut(s) 72, 305, 440
BssMI GATC 3 cut(s) 280, 372, 420
BssT1I CCWWGG 1 cut(s) 305
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 130
BstDEI CTNAG 1 cut(s) 53
BstF5I GGATG 1 cut(s) 143
BstFNI CGCG 1 cut(s) 289
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 3 cut(s) 283, 375, 423
BstMBI GATC 3 cut(s) 280, 372, 420
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 2 cut(s) 248, 307
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 1 cut(s) 71
BstSLI GKGCMC 1 cut(s) 333
BstUI CGCG 1 cut(s) 289
BstV1I GCAGC 3 cut(s) 133, 229, 322
BstV2I GAAGAC 1 cut(s) 387
Bsu36I CCTNAGG 1 cut(s) 53
BsuI GTATCC 1 cut(s) 447
BsuRI GGCC 2 cut(s) 47, 71
BtsCI GGATG 1 cut(s) 143
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
CciI TCATGA 2 cut(s) 39, 87
CfoI GCGC 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 46
CviAII CATG 4 cut(s) 40, 88, 94, 133
CviJI RGCY 9 cut(s) 47, 71, 177, 189, 232, 242, 301, 313, 453
CviKI_1 RGCY 9 cut(s) 47, 71, 177, 189, 232, 242, 301, 313, 453
DdeI CTNAG 1 cut(s) 53
DpnI GATC 3 cut(s) 282, 374, 422
DpnII GATC 3 cut(s) 280, 372, 420
Eco130I CCWWGG 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 53
EcoO109I RGGNCCY 1 cut(s) 46
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 71
EcoT14I CCWWGG 1 cut(s) 305
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 4 cut(s) 43, 91, 97, 136
FaiI YATR 7 cut(s) 41, 89, 95, 134, 236, 341, 456
FaqI GGGAC 1 cut(s) 140
FatI CATG 4 cut(s) 39, 87, 93, 132
FauI CCCGC 1 cut(s) 316
Fnu4HI GCNGC 3 cut(s) 147, 243, 311
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 147, 243, 311
FspBI CTAG 4 cut(s) 171, 252, 306, 465
GlaI GCGC 1 cut(s) 288
GluI GCNGC 3 cut(s) 147, 243, 311
HaeIII GGCC 2 cut(s) 47, 71
HapII CCGG 2 cut(s) 108, 317
HhaI GCGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 43, 91, 97, 136
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 3 cut(s) 27, 114, 363
HpaII CCGG 2 cut(s) 108, 317
Hpy188I TCNGA 2 cut(s) 368, 441
Hpy188III TCNNGA 4 cut(s) 40, 88, 418, 424
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 2 cut(s) 37, 363
HpyCH4III ACNGT 1 cut(s) 130
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 307
HpyF3I CTNAG 1 cut(s) 53
Hsp92II CATG 4 cut(s) 43, 91, 97, 136
HspAI GCGC 1 cut(s) 287
Kzo9I GATC 3 cut(s) 280, 372, 420
LmnI GCTCC 5 cut(s) 110, 229, 255, 306, 450
LpnPI CCDG 7 cut(s) 58, 85, 121, 156, 211, 269, 330
Lsp1109I GCAGC 3 cut(s) 133, 229, 322
LweI GCATC 1 cut(s) 128
MaeI CTAG 4 cut(s) 171, 252, 306, 465
MalI GATC 3 cut(s) 282, 374, 422
MbiI CCGCTC 1 cut(s) 431
MboI GATC 3 cut(s) 280, 372, 420
MboII GAAGA 6 cut(s) 16, 230, 392, 416, 419, 422
MhlI GDGCHC 2 cut(s) 107, 333
MluCI AATT 4 cut(s) 270, 275, 395, 475
MlyI GAGTC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 4 cut(s) 231, 324, 354, 435
MseI TTAA 1 cut(s) 62
MslI CAYNNNNRTG 2 cut(s) 92, 131
MspI CCGG 2 cut(s) 108, 317
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 1 cut(s) 289
MwoI GCNNNNNNNGC 2 cut(s) 248, 307
NdeII GATC 3 cut(s) 280, 372, 420
NlaIII CATG 4 cut(s) 43, 91, 97, 136
NlaIV GGNNCC 1 cut(s) 48
PagI TCATGA 2 cut(s) 39, 87
PfeI GAWTC 2 cut(s) 114, 363
PkrI GCNGC 3 cut(s) 148, 244, 312
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 92, 131
SaqAI TTAA 1 cut(s) 62
SatI GCNGC 3 cut(s) 147, 243, 311
Sau3AI GATC 3 cut(s) 280, 372, 420
Sau96I GGNCC 1 cut(s) 46
SchI GAGTC 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 2 cut(s) 107, 333
SetI ASST 7 cut(s) 13, 179, 191, 234, 303, 346, 455
SfaNI GCATC 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 92, 131
Sse9I AATT 4 cut(s) 270, 275, 395, 475
SsiI CCGC 2 cut(s) 323, 431
SspMI CTAG 4 cut(s) 171, 252, 306, 465
StyD4I CCNGG 1 cut(s) 71
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 130
TaqI TCGA 5 cut(s) 112, 117, 279, 352, 375
TaqII GACCGA 1 cut(s) 113
TasI AATT 4 cut(s) 270, 275, 395, 475
TfiI GAWTC 2 cut(s) 114, 363
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TscAI CASTG 1 cut(s) 211
TseI GCWGC 3 cut(s) 146, 242, 310
TspDTI ATGAA 4 cut(s) 17, 28, 56, 417
TspRI CASTG 1 cut(s) 211
XapI RAATTY 2 cut(s) 275, 395
XmaJI CCTAGG 1 cut(s) 305
XspI CTAG 4 cut(s) 171, 252, 306, 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.