Rorug05G0533500

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000103
Physical Location & Seq
Reverse (-)
72143976 .. 72151028
7053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0533500.1

Sequence Viewer

Length: 1740 bp
ATGGCTTCCCTTGCCCAAAACAACAGCAGGCTTTCCATAGCCATGGAGCGAACTGGCCAATGGATTTTCTCTCAAGATATCCCTGCAGATGTAGTAGTCGAAGTTGGCGAAGCCAATTTCTCCTTACACAAGTTCATGCTAGTGGCCAAGAGCAACCGGATTAGGCAACTGATATTGGAATCGGCCGAACCAGACCTGACCCGAATCGACCTCTCCAACATTCCTGGAGGACCGGAGACGTTCGAGAAGGCAGCCAAGTTCTGCTACGGCGTCAACTTCGAAATCACAGTCCACAACGTAGCGGCTCTCCGATGCGCCGCCGAGTACCTCCAAATGACCGATAAGTACTGCGATAACAACCTTACCGGCCGTACGGAAGATTTCCTCACTCAGGTGGCCTTATCCAGCCTCTCCGGCGCCATTGTCGTTCTCAAATCATGCGAAGACCTCACTCCCATCGCTGAAGAACTCAAAATCGTTCAGAGATGTGTGGAAGTAGTCACTTCCAAGGTTACAATCGAGGCAAACTTTCCGAGCCGGTCTCCGACGAACTGGTGGACGGAGGAGCTATCAATTTTGGACATTGATTTCTTTGGGAGAGTAATAACATCCATGAAACAACGTGCTTCGAAAACTCTGACCGTATCGAGTGCGATCGTTACATACGCCGAGAGATGGCTCCGGGATCTGATCCGGGACCACTCCGGCGCCGGCACCAAGTCCTTGGAGTCCGACGACTCCGACATCAGAATCCGGCAGCGGGAGCTTATACAATCCATAGTGCCTCTCCTTCCTTCCGAGAAGGCCGCTCTGCCGATCAACTTCATCTGCTGCCTTCTCAGATCAGCGACGTTCGTCAAGGCCTCGACGGCGACCAAGAACGAGCTAGAGAAGCGGATCTCGGCGATCTTGGAGCACGTAACCGTCGACGACCTGCTGGTCCTGTCGTTTTCTTACGACGGCGAGAGGCTGTTCGATCTGGAGAGCGTGAGGAGGATTATCTCGGGATTCGCGGAGAGGGAGAAGAGCGTGGCGGTTTTTAACGCCGGTGACTTCAGGGAGGTGTGCTCCGCTGCAATGCTCAGAGTTGCTAAGATTGTGGATGCGTATCTCGGCGAGATAGCCACTTGTACTGAGCTTAGCATATCCAAGTTTAATGGCATCGCCGTCCTCATCCCCAAAGGCGCGCGTAAAGTCGACGACGACCTCTATCGTGCCGTTGATATTTACTTGAAGTCTCATCCGAACCTTGATGAGATAGAGAGGGAGAAAGTGTGCAGTGTAATGGATGCATTGAAGTTGTCATACGAAGCGAGATTGCATGCTTCGCAAAATAAGCGTTTGCCTGTACAGATTGTGCTGCATGCGCTCTATTTCGACCAGCTCAAGCTGAGGAGCGGTGAAGACGAGGACGACATGCAAATGGATGCTGTATCGACCAGAAATCAGGTACAGGCCGACGTGTCGTTGGCTAAGGAGAATGAGGCACTGCGAACAGAGCTGTTGAAGATGAAGATGTACATTTCGGACATAAAAAAGACTGGAAGTGGTGTAGGTGCAAGTGCAGCAACGACGTCGTCTGGGAAAGGCGGTGGGCCTAGAAAGCCGACGTTTTTCTCGTCGGTGTCGAAGAAACTGGGAAAGTTGAATCCGTTCAAAAATGGGTCTAAGGACACCTTGAACATTGATGATTGTGTGGACGTTACCAAGCCTAGAAGAAGAAGGTTTTCCATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

579

Amino Acids

64.36

Weight (kDa)

6.46

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 24 - 114 7.6e-08 BTB/POZ domain
NPH3 PF03000 184 - 438 4.6e-76 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000103)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03461 FvH4_2g03490 FvH4_2g03500 FvH4_2g03500 FvH4_2g03510 FvH4_2g03510 FvH4_2g03521 FvH4_2g03521 FvH4_2g27100 FvH4_2g27110 FvH4_2g27130 FvH4_2g27150 FvH4_2g40420 FvH4_2g40420 FvH4_2g40420 FvH4_2g40420 FvH4_3g20160 FvH4_3g20160 FvH4_3g33042 FvH4_4g17790 FvH4_5g14272 FvH4_5g14273 FvH4_5g20540
malus_domestica MD00G1039500.v1.1 MD05G1103500.v1.1 MD05G1103900.v1.1 MD05G1104300.v1.1 MD05G1104400.v1.1 MD05G1104600.v1.1 MD05G1157200.v1.1 MD05G1157700.v1.1 MD05G1157800.v1.1 MD08G1037400.v1.1 MD10G1108900.v1.1
prunus_persica Prupe.1G384700_v2.0.a1 Prupe.1G384700_v2.0.a1 Prupe.8G148700_v2.0.a1 Prupe.8G148700_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G149000_v2.0.a1
pyrus_communis pycom05g10100 pycom05g14980 pycom08g02980 pycom10g09400 pycom15g02910
rosa_chinensis RchiOBHm_Chr3g0455511 RchiOBHm_Chr4g0421851 RchiOBHm_Chr4g0421921 RchiOBHm_Chr4g0421931 RchiOBHm_Chr5g0033951 RchiOBHm_Chr5g0033971 RchiOBHm_Chr6g0248641 RchiOBHm_Chr6g0248651 RchiOBHm_Chr6g0248671 RchiOBHm_Chr6g0248681 RchiOBHm_Chr6g0248731 RchiOBHm_Chr6g0248741 RchiOBHm_Chr6g0248771 RchiOBHm_Chr6g0248791 RchiOBHm_Chr6g0248851 RchiOBHm_Chr6g0248871 RchiOBHm_Chr6g0248881 RchiOBHm_Chr6g0248921 RchiOBHm_Chr6g0252081 RchiOBHm_Chr6g0252091 RchiOBHm_Chr6g0252101 RchiOBHm_Chr6g0267131 RchiOBHm_Chr6g0295981 RchiOBHm_Chr6g0295991 RchiOBHm_Chr6g0296021 RchiOBHm_Chr6g0300311 RchiOBHm_Chr7g0182631
rosa_laevigata RLG00000005114 RLG00000007652 RLG00000011348 RLG00000011741 RLG00000011742 RLG00000011743 RLG00000011744 RLG00000011745 RLG00000011747 RLG00000011748 RLG00000011749 RLG00000011750 RLG00000014028 RLG00000014036 RLG00000015111 RLG00000015113 RLG00000015115 RLG00000015117 RLG00000015119 RLG00000015123 RLG00000015124 RLG00000015127 RLG00000015128 RLG00000015129 RLG00000015132 RLG00000033513 RLG00000034608
rosa_multiflora Rmu_co8062356.1_g000001 Rmu_co8494777.1_g000001 Rmu_sc0000229.1_g000003 Rmu_sc0000529.1_g000001 Rmu_sc0000529.1_g000002 Rmu_sc0000529.1_g000003 Rmu_sc0001014.1_g000001 Rmu_sc0001150.1_g000007 Rmu_sc0001562.1_g000002 Rmu_sc0001562.1_g000004 Rmu_sc0001562.1_g000005 Rmu_sc0001562.1_g000017 Rmu_sc0001562.1_g000033 Rmu_sc0001562.1_g000035 Rmu_sc0001670.1_g000022 Rmu_sc0002393.1_g000007 Rmu_sc0002393.1_g000008 Rmu_sc0002393.1_g000009 Rmu_sc0002393.1_g000010 Rmu_sc0002393.1_g000011 Rmu_sc0002393.1_g000021 Rmu_sc0002393.1_g000023 Rmu_sc0002393.1_g000035 Rmu_sc0002393.1_g000037 Rmu_sc0002489.1_g000003 Rmu_sc0002938.1_g000047 Rmu_sc0003139.1_g000013 Rmu_sc0003139.1_g000014 Rmu_sc0003139.1_g000015 Rmu_sc0003139.1_g000017 Rmu_sc0004275.1_g000033 Rmu_sc0004275.1_g000036 Rmu_sc0004703.1_g000001 Rmu_sc0006276.1_g000003 Rmu_sc0010676.1_g000003 Rmu_sc0010676.1_g000004 Rmu_sc0010676.1_g000006 Rmu_sc0010676.1_g000007 Rmu_sc0013953.1_g000004 Rmu_ssc0000388.1_g000016
rosa_roxburghii Rroxscaffold_1G00015220 Rroxscaffold_1G00046270 Rroxscaffold_2G00090150 Rroxscaffold_2G00120650 Rroxscaffold_2G00123380 Rroxscaffold_3G00234080 Rroxscaffold_3G00271110 Rroxscaffold_7G00167900 Rroxscaffold_7G00171950 Rroxscaffold_7G00171970 Rroxscaffold_7G00171980 Rroxscaffold_7G00171990 Rroxscaffold_7G00172000 Rroxscaffold_7G00201050 Rroxscaffold_7G00212860 Rroxscaffold_7G00212880 Rroxscaffold_7G00212920 Rroxscaffold_7G00212940 Rroxscaffold_7G00212990 Rroxscaffold_7G00213060 Rroxscaffold_7G00213070 Rroxscaffold_7G00213080 Rroxscaffold_7G00213100 Rroxscaffold_7G00213110 Rroxscaffold_7G00213120 Rroxscaffold_7G00213130 Rroxscaffold_7G00213140 Rroxscaffold_7G00217950
rosa_rugosa Rorug04G0089100 Rorug04G0176100 Rorug05G0143700 Rorug05G0532600 Rorug05G0532700 Rorug05G0532800 Rorug05G0533100 Rorug05G0533200 Rorug05G0533300 Rorug05G0533400 Rorug05G0533500 Rorug05G0533500 Rorug05G0533700 Rorug05G0533800 Rorug05G0533900 Rorug05G0534000 Rorug05G0534100.1 Rorug05G0534200 Rorug06G0029200 Rorug06G0029300 Rorug06G0029400 Rorug06G0255600 Rorug06G0255800 Rorug06G0255800 Rorug06G0255900 Rorug06G0255900 Rorug06G0255900 Rorug06G0256000 Rorug06G0256100 Rorug06G0256300 Rorug06G0256400 Rorug06G0294900 Rorug06G0448600 Rorug06G0448600 Rorug07G0094000
rosa_samantha Rh3BG070200 Rh3CG325900 Rh5BG235500 Rh6AG049500 Rh6AG049600 Rh6AG049800 Rh6AG049900 Rh6AG050100 Rh6AG050200 Rh6AG050300 Rh6AG050400 Rh6AG050500 Rh6AG050800 Rh6AG051100 Rh6AG051500 Rh6AG151100 Rh6AG369300 Rh6AG369500 Rh6AG369600 Rh6AG369800 Rh6AG369900 Rh6AG407600 Rh6DG038100 Rh7CG053500 Rh7CG053600 Rh7DG051800
rosa_wichuraiana Rw3G005370 Rw3G025850 Rw5G021490 Rw6G004290 Rw6G004300 Rw6G004310 Rw6G004320 Rw6G004330 Rw6G004340 Rw6G004390 Rw6G004410 Rw6G004420 Rw6G004430 Rw6G004450 Rw6G004460 Rw6G004490 Rw6G004510 Rw6G004590 Rw6G013080 Rw6G032210 Rw6G032220 Rw6G032230 Rw6G032250 Rw6G035640 Rw7G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 938
AatII GACGTC 1 cut(s) 1577
Acc36I ACCTGC 1 cut(s) 942
AccB1I GGYRCC 3 cut(s) 416, 707, 713
AccBSI CCGCTC 2 cut(s) 809, 1398
AccI GTMKAC 2 cut(s) 927, 1197
AccII CGCG 3 cut(s) 1013, 1187, 1189
AclWI GGATC 3 cut(s) 685, 693, 905
AcoI YGGCCR 4 cut(s) 55, 144, 183, 367
AcuI CTGAAG 2 cut(s) 483, 1039
AcyI GRCGYC 4 cut(s) 270, 417, 708, 1574
AfaI GTAC 7 cut(s) 326, 347, 373, 1132, 1350, 1452, 1520
AfiI CCNNNNNNNGG 3 cut(s) 391, 675, 760
AflIII ACRYGT 1 cut(s) 1461
AgsI TTSAA 6 cut(s) 1234, 1297, 1507, 1648, 1657, 1681
AjiI CACGTC 1 cut(s) 1462
AjnI CCWGG 1 cut(s) 223
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 7 cut(s) 568, 766, 886, 1138, 1384, 1390, 1501
AluI AGCT 7 cut(s) 568, 766, 886, 1138, 1384, 1390, 1501
Alw21I GWGCWC 2 cut(s) 918, 1070
Alw26I GTCTC 3 cut(s) 230, 546, 1242
AlwI GGATC 3 cut(s) 685, 693, 905
Ama87I CYCGRG 1 cut(s) 1003
AoxI GGCC 9 cut(s) 55, 144, 183, 367, 396, 804, 861, 1455, 1595
ApeKI GCWGC 6 cut(s) 251, 757, 831, 1073, 1360, 1565
ArsI GACNNNNNNTTYG 2 cut(s) 273, 305
Asp700I GAANNNNTTC 2 cut(s) 1652, 1726
AspLEI GCGC 6 cut(s) 317, 419, 710, 1187, 1189, 1369
AspS9I GGNCC 4 cut(s) 230, 697, 940, 1595
AsuC2I CCSGG 2 cut(s) 683, 695
AsuHPI GGTGA 2 cut(s) 1061, 1412
AsuII TTCGAA 2 cut(s) 279, 629
AvaI CYCGRG 1 cut(s) 1003
AvaII GGWCC 3 cut(s) 230, 697, 940
BalI TGGCCA 2 cut(s) 57, 146
BanI GGYRCC 3 cut(s) 416, 707, 713
BarI GAAGNNNNNNTAC 2 cut(s) 1289, 1321
BbsI GAAGAC 2 cut(s) 450, 1410
Bbv12I GWGCWC 2 cut(s) 918, 1070
BbvCI CCTCAGC 1 cut(s) 1391
BbvI GCAGC 6 cut(s) 263, 769, 818, 1060, 1347, 1577
BccI CCATC 3 cut(s) 464, 669, 1739
BceAI ACGGC 6 cut(s) 283, 354, 885, 976, 1151, 1202
BcgI CGANNNNNNTGC 2 cut(s) 1557, 1591
BciT130I CCWGG 1 cut(s) 225
BcnI CCSGG 2 cut(s) 683, 695
BcoDI GTCTC 3 cut(s) 230, 546, 1242
BfaI CTAG 4 cut(s) 140, 887, 1599, 1713
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 2 cut(s) 420, 711
BfuAI ACCTGC 1 cut(s) 942
BglI GCCNNNNNGGC 2 cut(s) 414, 869
BisI GCNGC 9 cut(s) 252, 303, 318, 758, 807, 832, 1074, 1361, 1566
BlpI GCTNAGC 1 cut(s) 1139
BlsI GCNGC 9 cut(s) 253, 304, 319, 759, 808, 833, 1075, 1362, 1567
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 3 cut(s) 225, 683, 695
Bme18I GGWCC 3 cut(s) 230, 697, 940
BmeT110I CYCGRG 1 cut(s) 1003
BmgBI CACGTC 1 cut(s) 1462
BmgT120I GGNCC 4 cut(s) 230, 697, 940, 1595
BmiI GGNNCC 5 cut(s) 418, 680, 698, 709, 715
BmrFI CCNGG 3 cut(s) 225, 683, 695
BmrI ACTGGG 1 cut(s) 1646
BmsI GCATC 5 cut(s) 302, 1093, 1170, 1279, 1417
BmuI ACTGGG 1 cut(s) 1646
BoxI GACNNNNGTC 1 cut(s) 854
BpiI GAAGAC 2 cut(s) 450, 1410
BplI GAGNNNNNCTC 4 cut(s) 526, 558, 1052, 1084
BpmI CTGGAG 2 cut(s) 246, 1001
Bpu10I CCTNAGC 2 cut(s) 1391, 1473
Bpu1102I GCTNAGC 1 cut(s) 1139
Bpu14I TTCGAA 2 cut(s) 279, 629
BpuEI CTTGAG 2 cut(s) 57, 1370
BpuMI CCSGG 2 cut(s) 683, 695
BsaAI YACGTR 1 cut(s) 919
BsaBI GATNNNNATC 1 cut(s) 1107
BsaHI GRCGYC 4 cut(s) 270, 417, 708, 1574
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 3 cut(s) 42, 507, 723
BsaWI WCCGGW 2 cut(s) 156, 232
BsaXI ACNNNNNCTCC 2 cut(s) 1259, 1289
Bsc4I CCNNNNNNNGG 3 cut(s) 391, 675, 760
Bse118I RCCGGY 4 cut(s) 365, 537, 710, 1046
Bse1I ACTGG 4 cut(s) 58, 557, 1546, 1641
Bse3DI GCAATG 1 cut(s) 1083
Bse8I GATNNNNATC 1 cut(s) 1107
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 42, 507, 723
BseGI GGATG 6 cut(s) 608, 1108, 1173, 1240, 1294, 1432
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 3 cut(s) 391, 675, 760
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 5 cut(s) 404, 853, 1096, 1125, 1382
BseNI ACTGG 4 cut(s) 58, 557, 1546, 1641
BsePI GCGCGC 1 cut(s) 1185
BseRI GAGGAG 3 cut(s) 578, 1006, 1408
BseX3I CGGCCG 2 cut(s) 183, 367
BseXI GCAGC 6 cut(s) 263, 769, 818, 1060, 1347, 1577
BsgI GTGCAG 2 cut(s) 1297, 1584
Bsh1236I CGCG 3 cut(s) 1013, 1187, 1189
Bsh1285I CGRYCG 3 cut(s) 186, 370, 657
BshFI GGCC 9 cut(s) 57, 146, 185, 369, 398, 806, 863, 1457, 1597
BshNI GGYRCC 3 cut(s) 416, 707, 713
BsiEI CGRYCG 3 cut(s) 186, 370, 657
BsiHKAI GWGCWC 2 cut(s) 918, 1070
BsiHKCI CYCGRG 1 cut(s) 1003
BsiWI CGTACG 1 cut(s) 371
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 3 cut(s) 391, 675, 760
BsmAI GTCTC 3 cut(s) 230, 546, 1242
BsmBI CGTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 710
BsnI GGCC 9 cut(s) 57, 146, 185, 369, 398, 806, 863, 1457, 1597
Bso31I GGTCTC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 1003
Bsp119I TTCGAA 2 cut(s) 279, 629
Bsp1286I GDGCHC 2 cut(s) 918, 1070
Bsp1407I TGTACA 2 cut(s) 1348, 1518
Bsp143I GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
Bsp1720I GCTNAGC 1 cut(s) 1139
Bsp19I CCATGG 1 cut(s) 42
BspANI GGCC 9 cut(s) 57, 146, 185, 369, 398, 806, 863, 1457, 1597
BspCNI CTCAG 5 cut(s) 403, 852, 1095, 1126, 1383
BspFNI CGCG 3 cut(s) 1013, 1187, 1189
BspLI GGNNCC 5 cut(s) 418, 680, 698, 709, 715
BspMAI CTGCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 942
BspPI GGATC 3 cut(s) 685, 693, 905
BspQI GCTCTTC 1 cut(s) 1019
BspT104I TTCGAA 2 cut(s) 279, 629
BspT107I GGYRCC 3 cut(s) 416, 707, 713
BspTNI GGTCTC 1 cut(s) 546
BsrBI CCGCTC 2 cut(s) 809, 1398
BsrDI GCAATG 1 cut(s) 1083
BsrFI RCCGGY 4 cut(s) 365, 537, 710, 1046
BsrGI TGTACA 2 cut(s) 1348, 1518
BsrI ACTGG 4 cut(s) 58, 557, 1546, 1641
BssAI RCCGGY 4 cut(s) 365, 537, 710, 1046
BssECI CCNNGG 3 cut(s) 42, 507, 723
BssHII GCGCGC 1 cut(s) 1185
BssMI GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
BssNI GRCGYC 4 cut(s) 270, 417, 708, 1574
BssT1I CCWWGG 3 cut(s) 42, 507, 723
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 3 cut(s) 289, 643, 925
Bst6I CTCTTC 1 cut(s) 1019
BstACI GRCGYC 4 cut(s) 270, 417, 708, 1574
BstAUI TGTACA 2 cut(s) 1348, 1518
BstBAI YACGTR 1 cut(s) 919
BstBI TTCGAA 2 cut(s) 279, 629
BstC8I GCNNGC 5 cut(s) 29, 712, 1187, 1323, 1365
BstDEI CTNAG 9 cut(s) 390, 839, 1082, 1092, 1134, 1139, 1391, 1473, 1668
BstDSI CCRYGG 1 cut(s) 42
BstENI CCTNNNNNAGG 1 cut(s) 389
BstF5I GGATG 6 cut(s) 608, 1108, 1173, 1240, 1294, 1432
BstFNI CGCG 3 cut(s) 1013, 1187, 1189
BstH2I RGCGCY 2 cut(s) 420, 711
BstHHI GCGC 6 cut(s) 317, 419, 710, 1187, 1189, 1369
BstKTI GATC 8 cut(s) 657, 688, 693, 819, 845, 900, 909, 979
BstMAI GTCTC 3 cut(s) 230, 546, 1242
BstMBI GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
BstMCI CGRYCG 3 cut(s) 186, 370, 657
BstNI CCWGG 1 cut(s) 225
BstNSI RCATGY 3 cut(s) 1325, 1367, 1420
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 3 cut(s) 223, 681, 693
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 3 cut(s) 1013, 1187, 1189
BstV1I GCAGC 6 cut(s) 263, 769, 818, 1060, 1347, 1577
BstV2I GAAGAC 2 cut(s) 450, 1410
BstX2I RGATCY 2 cut(s) 685, 897
BstXI CCANNNNNNTGG 2 cut(s) 43, 724
BstYI RGATCY 2 cut(s) 685, 897
BstZI CGGCCG 2 cut(s) 183, 367
BsuRI GGCC 9 cut(s) 57, 146, 185, 369, 398, 806, 863, 1457, 1597
BtgI CCRYGG 1 cut(s) 42
BtgZI GCGATG 2 cut(s) 442, 1147
BtrI CACGTC 1 cut(s) 1462
BtsCI GGATG 6 cut(s) 608, 1108, 1173, 1240, 1294, 1432
BtsI GCAGTG 2 cut(s) 1285, 1487
BtsIMutI CAGTG 2 cut(s) 1285, 1487
BveI ACCTGC 1 cut(s) 942
Cac8I GCNNGC 5 cut(s) 29, 712, 1187, 1323, 1365
CfoI GCGC 6 cut(s) 317, 419, 710, 1187, 1189, 1369
Cfr10I RCCGGY 4 cut(s) 365, 537, 710, 1046
Cfr13I GGNCC 4 cut(s) 230, 697, 940, 1595
CseI GACGC 1 cut(s) 259
Csp6I GTAC 7 cut(s) 325, 346, 372, 1131, 1349, 1451, 1519
CviAII CATG 7 cut(s) 43, 136, 438, 613, 1322, 1364, 1417
CviQI GTAC 7 cut(s) 325, 346, 372, 1131, 1349, 1451, 1519
DdeI CTNAG 9 cut(s) 390, 839, 1082, 1092, 1134, 1139, 1391, 1473, 1668
DinI GGCGCC 2 cut(s) 418, 709
DpnI GATC 8 cut(s) 656, 687, 692, 818, 844, 899, 908, 978
DpnII GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
DrdI GACNNNNNNGTC 1 cut(s) 938
DseDI GACNNNNNNGTC 1 cut(s) 938
EaeI YGGCCR 4 cut(s) 55, 144, 183, 367
EagI CGGCCG 2 cut(s) 183, 367
Eam1104I CTCTTC 1 cut(s) 1019
EarI CTCTTC 1 cut(s) 1019
EclXI CGGCCG 2 cut(s) 183, 367
Eco130I CCWWGG 3 cut(s) 42, 507, 723
Eco147I AGGCCT 1 cut(s) 863
Eco31I GGTCTC 1 cut(s) 546
Eco32I GATATC 1 cut(s) 79
Eco47I GGWCC 3 cut(s) 230, 697, 940
Eco52I CGGCCG 2 cut(s) 183, 367
Eco57I CTGAAG 2 cut(s) 483, 1039
Eco88I CYCGRG 1 cut(s) 1003
EcoNI CCTNNNNNAGG 1 cut(s) 389
EcoRII CCWGG 1 cut(s) 223
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 3 cut(s) 42, 507, 723
EcoT22I ATGCAT 1 cut(s) 1294
EgeI GGCGCC 2 cut(s) 418, 709
EheI GGCGCC 2 cut(s) 418, 709
ErhI CCWWGG 3 cut(s) 42, 507, 723
Esp3I CGTCTC 1 cut(s) 230
FaeI CATG 7 cut(s) 46, 139, 441, 616, 1325, 1367, 1420
FalI AAGNNNNNCTT 2 cut(s) 1661, 1693
FaqI GGGAC 1 cut(s) 710
FatI CATG 7 cut(s) 42, 135, 437, 612, 1321, 1363, 1416
FauI CCCGC 1 cut(s) 753
FblI GTMKAC 2 cut(s) 927, 1197
Fnu4HI GCNGC 9 cut(s) 252, 303, 318, 758, 807, 832, 1074, 1361, 1566
FokI GGATG 6 cut(s) 595, 1115, 1160, 1227, 1301, 1439
Fsp4HI GCNGC 9 cut(s) 252, 303, 318, 758, 807, 832, 1074, 1361, 1566
FspBI CTAG 4 cut(s) 140, 887, 1599, 1713
GlaI GCGC 6 cut(s) 316, 418, 709, 1186, 1188, 1368
GluI GCNGC 9 cut(s) 252, 303, 318, 758, 807, 832, 1074, 1361, 1566
GsuI CTGGAG 2 cut(s) 246, 1001
HaeII RGCGCY 2 cut(s) 420, 711
HaeIII GGCC 9 cut(s) 57, 146, 185, 369, 398, 806, 863, 1457, 1597
HgaI GACGC 1 cut(s) 259
HhaI GCGC 6 cut(s) 317, 419, 710, 1187, 1189, 1369
Hin1I GRCGYC 4 cut(s) 270, 417, 708, 1574
Hin1II CATG 7 cut(s) 46, 139, 441, 616, 1325, 1367, 1420
Hin6I GCGC 6 cut(s) 315, 417, 708, 1185, 1187, 1367
HinP1I GCGC 6 cut(s) 315, 417, 708, 1185, 1187, 1367
HincII GTYRAC 3 cut(s) 274, 928, 1198
HindII GTYRAC 3 cut(s) 274, 928, 1198
HinfI GANTC 7 cut(s) 179, 204, 728, 737, 750, 1008, 1648
HphI GGTGA 2 cut(s) 1061, 1412
Hpy166II GTNNAC 6 cut(s) 274, 292, 558, 928, 1198, 1699
Hpy188III TCNNGA 4 cut(s) 74, 244, 980, 1005
Hpy8I GTNNAC 6 cut(s) 274, 292, 558, 928, 1198, 1699
HpyAV CCTTC 6 cut(s) 241, 796, 800, 804, 845, 1716
HpyCH4III ACNGT 3 cut(s) 289, 643, 925
HpyCH4IV ACGT 9 cut(s) 239, 297, 622, 851, 918, 1461, 1574, 1610, 1701
HpyCH4V TGCA 9 cut(s) 86, 1076, 1278, 1292, 1321, 1363, 1420, 1559, 1565
HpyF3I CTNAG 9 cut(s) 390, 839, 1082, 1092, 1134, 1139, 1391, 1473, 1668
HpySE526I ACGT 9 cut(s) 239, 297, 622, 851, 918, 1461, 1574, 1610, 1701
Hsp92I GRCGYC 4 cut(s) 270, 417, 708, 1574
Hsp92II CATG 7 cut(s) 46, 139, 441, 616, 1325, 1367, 1420
HspAI GCGC 6 cut(s) 315, 417, 708, 1185, 1187, 1367
KasI GGCGCC 2 cut(s) 416, 707
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
Kzo9I GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
LguI GCTCTTC 1 cut(s) 1019
LmnI GCTCC 7 cut(s) 46, 565, 684, 763, 913, 1073, 1395
Lsp1109I GCAGC 6 cut(s) 263, 769, 818, 1060, 1347, 1577
LweI GCATC 5 cut(s) 302, 1093, 1170, 1279, 1417
MaeI CTAG 4 cut(s) 140, 887, 1599, 1713
MaeII ACGT 9 cut(s) 239, 297, 622, 851, 918, 1461, 1574, 1610, 1701
MaeIII GTNAC 6 cut(s) 499, 511, 658, 919, 1049, 1702
MalI GATC 8 cut(s) 656, 687, 692, 818, 844, 899, 908, 978
MbiI CCGCTC 2 cut(s) 809, 1398
MboI GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
MflI RGATCY 2 cut(s) 685, 897
MhlI GDGCHC 2 cut(s) 918, 1070
MlsI TGGCCA 2 cut(s) 57, 146
MluCI AATT 2 cut(s) 115, 573
MluNI TGGCCA 2 cut(s) 57, 146
Mly113I GGCGCC 2 cut(s) 417, 708
MlyI GAGTC 2 cut(s) 731, 737
MmeI TCCRAC 4 cut(s) 240, 569, 756, 765
Mox20I TGGCCA 2 cut(s) 57, 146
Mph1103I ATGCAT 1 cut(s) 1294
MroNI GCCGGC 1 cut(s) 710
MroXI GAANNNNTTC 2 cut(s) 1652, 1726
MscI TGGCCA 2 cut(s) 57, 146
MseI TTAA 3 cut(s) 1041, 1155, 1738
MslI CAYNNNNRTG 4 cut(s) 41, 140, 392, 1421
Msp20I TGGCCA 2 cut(s) 57, 146
MspA1I CMGCKG 2 cut(s) 760, 1073
MspR9I CCNGG 3 cut(s) 225, 683, 695
MvaI CCWGG 1 cut(s) 225
MvnI CGCG 3 cut(s) 1013, 1187, 1189
NaeI GCCGGC 1 cut(s) 712
NarI GGCGCC 2 cut(s) 417, 708
NciI CCSGG 2 cut(s) 683, 695
NcoI CCATGG 1 cut(s) 42
NdeII GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
NgoMIV GCCGGC 1 cut(s) 710
NlaIII CATG 7 cut(s) 46, 139, 441, 616, 1325, 1367, 1420
NlaIV GGNNCC 5 cut(s) 418, 680, 698, 709, 715
NmeAIII GCCGAG 4 cut(s) 346, 694, 881, 1092
NmuCI GTSAC 2 cut(s) 499, 1049
NsiI ATGCAT 1 cut(s) 1294
NspI RCATGY 3 cut(s) 1325, 1367, 1420
NspV TTCGAA 2 cut(s) 279, 629
OliI CACNNNNGTG 1 cut(s) 392
PaeI GCATGC 2 cut(s) 1325, 1367
PauI GCGCGC 1 cut(s) 1185
PceI AGGCCT 1 cut(s) 863
PciSI GCTCTTC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 924, 1616, 1625
PdiI GCCGGC 1 cut(s) 712
PdmI GAANNNNTTC 2 cut(s) 1652, 1726
PfeI GAWTC 5 cut(s) 179, 204, 750, 1008, 1648
Pfl23II CGTACG 1 cut(s) 371
PflFI GACNNNGTC 1 cut(s) 1576
PfoI TCCNGGA 3 cut(s) 223, 681, 693
PkrI GCNGC 9 cut(s) 253, 304, 319, 759, 808, 833, 1075, 1362, 1567
Ple19I CGATCG 1 cut(s) 657
PleI GAGTC 2 cut(s) 731, 736
PluTI GGCGCC 2 cut(s) 420, 711
PpsI GAGTC 2 cut(s) 731, 736
Ppu21I YACGTR 1 cut(s) 919
PshAI GACNNNNGTC 1 cut(s) 854
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspLI CGTACG 1 cut(s) 371
PspN4I GGNNCC 5 cut(s) 418, 680, 698, 709, 715
PspPI GGNCC 4 cut(s) 230, 697, 940, 1595
PstI CTGCAG 1 cut(s) 88
PsuI RGATCY 2 cut(s) 685, 897
PsyI GACNNNGTC 1 cut(s) 1576
PteI GCGCGC 1 cut(s) 1185
PvuI CGATCG 1 cut(s) 657
RsaI GTAC 7 cut(s) 326, 347, 373, 1132, 1350, 1452, 1520
RsaNI GTAC 7 cut(s) 325, 346, 372, 1131, 1349, 1451, 1519
RseI CAYNNNNRTG 4 cut(s) 41, 140, 392, 1421
SalI GTCGAC 2 cut(s) 926, 1196
SapI GCTCTTC 1 cut(s) 1019
SaqAI TTAA 3 cut(s) 1041, 1155, 1738
SatI GCNGC 9 cut(s) 252, 303, 318, 758, 807, 832, 1074, 1361, 1566
Sau3AI GATC 8 cut(s) 654, 685, 690, 816, 842, 897, 906, 976
Sau96I GGNCC 4 cut(s) 230, 697, 940, 1595
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 2 cut(s) 731, 737
ScrFI CCNGG 3 cut(s) 225, 683, 695
SduI GDGCHC 2 cut(s) 918, 1070
SfaNI GCATC 5 cut(s) 302, 1093, 1170, 1279, 1417
SfcI CTRYAG 1 cut(s) 84
SfoI GGCGCC 2 cut(s) 418, 709
SfuI TTCGAA 2 cut(s) 279, 629
SgrAI CRCCGGYG 1 cut(s) 1046
SgrDI CGTCGACG 1 cut(s) 926
SinI GGWCC 3 cut(s) 230, 697, 940
SmiMI CAYNNNNRTG 4 cut(s) 41, 140, 392, 1421
SmlI CTYRAG 2 cut(s) 72, 1385
SmoI CTYRAG 2 cut(s) 72, 1385
SphI GCATGC 2 cut(s) 1325, 1367
Sse9I AATT 2 cut(s) 115, 573
SseBI AGGCCT 1 cut(s) 863
SspDI GGCGCC 2 cut(s) 416, 707
SspMI CTAG 4 cut(s) 140, 887, 1599, 1713
StuI AGGCCT 1 cut(s) 863
StyD4I CCNGG 3 cut(s) 223, 681, 693
StyI CCWWGG 3 cut(s) 42, 507, 723
TaaI ACNGT 3 cut(s) 289, 643, 925
TaiI ACGT 9 cut(s) 242, 300, 625, 854, 921, 1464, 1577, 1613, 1704
TaqII GACCGA 1 cut(s) 353
TasI AATT 2 cut(s) 115, 573
TatI WGTACW 4 cut(s) 345, 1130, 1348, 1518
TauI GCSGC 3 cut(s) 305, 320, 809
TfiI GAWTC 5 cut(s) 179, 204, 750, 1008, 1648
Tru1I TTAA 3 cut(s) 1041, 1155, 1738
Tru9I TTAA 3 cut(s) 1041, 1155, 1738
TscAI CASTG 2 cut(s) 1285, 1494
TseFI GTSAC 2 cut(s) 499, 1049
TseI GCWGC 6 cut(s) 251, 757, 831, 1073, 1360, 1565
Tsp45I GTSAC 2 cut(s) 499, 1049
TspDTI ATGAA 4 cut(s) 124, 629, 814, 1526
TspGWI ACGGA 3 cut(s) 389, 575, 1641
TspRI CASTG 2 cut(s) 1285, 1494
Tth111I GACNNNGTC 1 cut(s) 1576
VpaK11BI GGWCC 3 cut(s) 230, 697, 940
XagI CCTNNNNNAGG 1 cut(s) 389
XceI RCATGY 3 cut(s) 1325, 1367, 1420
XmiI GTMKAC 2 cut(s) 927, 1197
XmnI GAANNNNTTC 2 cut(s) 1652, 1726
XspI CTAG 4 cut(s) 140, 887, 1599, 1713
ZraI GACGTC 1 cut(s) 1575
ZrmI AGTACT 1 cut(s) 347
Zsp2I ATGCAT 1 cut(s) 1294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.