Rroxscaffold_7G00212990

F-box FBD LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000103
Physical Location & Seq
Forward (+)
63530105 .. 63536187
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00212990.1

Sequence Viewer

Length: 2577 bp
ATGCCAGGGCAGGAGGGAAGCAGCTCACAAAGGGCACCACCATACGAAGTTACAAAGAGAGGAGAACAAGAACAGTCAAAAGTAGACTCTGGGACAGAACTAGATCAAGACGAAGCTCTGCCAAGATACGGGTCGATGCTTAGTGTGCTAGTACTGAAGATGGAGTTGGACAGAATTAGCAGTTTACCAAATGATGTCACAGAAAAGATTTTGTCATGTATGCCGCTTAGGGAAGCAGTGAGGACAAGTGTTTTATCCAGCAAGTGGAGGTACAAATCGGCCATGCTTCAAGATCTAAAATTTAATAAATATATGTGGAGGAGAAGCCAAAATCGGGAGGTGACAACCTTTGTGAACATGGTTGATCACGTACTCTTACTTCATATTGGCCCCATTCGCAAGTTCGAGCTGTTTGCTGATGAGTTAGCCACTAGAGATATTGATCGATGGCTTACTCATCTATCAAGAAACTCGATCAAAGAGCTGGTACTTCGTCTTTGGGTATCTGAATACCACATTCCATTATGTGTGTTTTCTTGTCAAGATCTCGTTCTTTTGGTGTTAACTCAATCTTTTCATACGGTGTCAAATTTAAAAAAGGTTTGGCTCAAACCTCCGTCGACGTTCAAAGGATTCAGGAGCTTGAAGAGCCTTCAACTTGTGGGCATCACCATGGCCCAACATGTGTTTGAAAATTTGACACGTTGCAGTCCTCTGCTTGAGAGATTAATATTGCGACATTGTGATGGTGTCACCACTCTCAATATTGATGCGCCAAATCTCAAATTTTTAGACATTTTAGGTGTCTTTCGTGTTGGCAATCTTAAAAGTACCCTAAATCTTACTGATGCTTTAATCGGGGTGAACATCTATACTGATGGTCCTAACCAAAGATGGGTTCCTGTCAGTTGGGTTCCTAGCGGTTCTAGCAATTTGCTCAAGATTTTTGATCGCCTCCCTCATATTCGAAGGCTCGCGATTAAGTTGCATTTTTTAGAGTATTTGTCTCTTGGTGCCTTACCAGAAAAGCTTCCTAAACCATGTGAATATCTGAATGTTCTTTCTATAGACATATGCTTTGATGATCCGGATGCTATTCTAACTGCTCTACGCATTCTGAGAAGCTCCCCTGCTCTGCAGGAATTAGAAATTTTTATGGATGGCAGTAAGAAACTCAGAAGGAGTCCCACTTTTATCGAAGAACAGAAATCTTCTTGTTTAAATGTGAGCTACAATTGCACACTTTCTCAACTGCGACTTGTGAAAATAACTGAGATCTCTGGTGTTAAAGCTGAACTAGATTTCATCAGATTTCTACTATTAAGTTCACCTGTGCTTGAGAGAATGACTGTCACGCCTCATGAATTTCCCGAGCCTTGTAGTGATGGACTTAAGAGTCTGCTGATACTGAAAATGGAGATGGATAGAATTAGCAGTTTACCAAATGATGTCACAGAAAAGATTTTGTCACGTTTGCCGCTTAGGGACGCGGTGAGGACAAGTGTTTTATCCAGCAGGTGGAGGTACAAGTCGGCCATGCTTCAAGATCTAGAATTTGATGATTATTATTGGGGGAGAAGCCAAAATCGAGAGTTTGATGATGATGATGATTGTCAAATCCACACAACCTTTGTGAACATGATTGATCATGTGCTCTTAGTTCATATCGTTCCCATACGCAAGTCTTGTTCTTTTGGGGTTAGTCAAGTGTTTGCTCAAACCTCCTCAACATTCAAAGGGTTCAAGAGCTTGAAGAGTCTTCAACTCCACGCTGTTGACGTTGCCCAAGATGTGTTTGAAAATTTGATTCGTTGCAGTCCTCTGCTTGAGAGATTAATATTCAGAAATTGTGATAGTGTCACCGATCTCAATATTGATGCACCAAATCTCAAATCTTTAGATATTTTCGGTGTCTTTGATGTTGTCAGCCTTAAAAGTACCCTAAATCTTACTGATGCTTCAATCTTTGTGACCATCCATTCTGATGGTCCTCACGATAGACGGGTTCCTGTCAGTTGGGTTCCTAGCAGTTCTAGCAATTTGCTCAGAATTTTTGATCACCTCCCTAATATTCAAAGGCTCGCAATTAAGTCGCACTTTTTAAAGTATTTGTCTCTTGGTGCCTTGCCCGAAAAGCTTCCTAAACCATGTGAATATCTGAATGTTCTTTCTATAGACATATGCTTTGATGACCCGGATGCAATTCTAACTGCTCTATGCATTCTGAGAAGCTCCCCTGCTCTACAGGAATTAGAAATTCTTATGGATCGGACAGAGAAACTTGAAAGGATACACTCTGTTGTCAAACAAGTGAAGTCTTCTTGTTTGGATGTGAGCTACAATTGCACACTTTCTCAACTGCGACTTGTGAAAATAACTGAGATCTCTGGTGTTAAAGCTGAACTACATTTCATCAGATTTCTACTTTTAAGTTCACCTCTGCTTGAGAGAATGACTATCAGGCCTCCTCGATTTCCTGTGCCTGCTACTGTGGCTGGTTTCCCAAAACTAGTAAAAGAGCTACTCCAGTTTAAACGTGAATCAAAGCATGCAGAGATTATTCTCCTGGATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD LRR_2

Protein Analysis

858

Amino Acids

97.81

Weight (kDa)

8.27

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 59 - 91 1.2e-06 F-box domain
LRR_At1g61320_AtMIF1 PF23622 140 - 461 1.9e-12 At1g61320/AtMIF1, LRR domain
LRR_At5g56370 PF24758 147 - 384 6.4e-12 FBD-associated F-box protein At5g56370, LRR repeats
FBD PF08387 413 - 450 1.3e-06 FBD
F-box PF00646 477 - 509 2e-07 F-box domain
LRR_At5g56370 PF24758 565 - 753 1.1e-10 FBD-associated F-box protein At5g56370, LRR repeats
LRR_At1g61320_AtMIF1 PF23622 575 - 820 2.2e-07 At1g61320/AtMIF1, LRR domain
FBD PF08387 782 - 820 1e-05 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000103)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03461 FvH4_2g03490 FvH4_2g03500 FvH4_2g03500 FvH4_2g03510 FvH4_2g03510 FvH4_2g03521 FvH4_2g03521 FvH4_2g27100 FvH4_2g27110 FvH4_2g27130 FvH4_2g27150 FvH4_2g40420 FvH4_2g40420 FvH4_2g40420 FvH4_2g40420 FvH4_3g20160 FvH4_3g20160 FvH4_3g33042 FvH4_4g17790 FvH4_5g14272 FvH4_5g14273 FvH4_5g20540
malus_domestica MD00G1039500.v1.1 MD05G1103500.v1.1 MD05G1103900.v1.1 MD05G1104300.v1.1 MD05G1104400.v1.1 MD05G1104600.v1.1 MD05G1157200.v1.1 MD05G1157700.v1.1 MD05G1157800.v1.1 MD08G1037400.v1.1 MD10G1108900.v1.1
prunus_persica Prupe.1G384700_v2.0.a1 Prupe.1G384700_v2.0.a1 Prupe.8G148700_v2.0.a1 Prupe.8G148700_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G148900_v2.0.a1 Prupe.8G149000_v2.0.a1
pyrus_communis pycom05g10100 pycom05g14980 pycom08g02980 pycom10g09400 pycom15g02910
rosa_chinensis RchiOBHm_Chr3g0455511 RchiOBHm_Chr4g0421851 RchiOBHm_Chr4g0421921 RchiOBHm_Chr4g0421931 RchiOBHm_Chr5g0033951 RchiOBHm_Chr5g0033971 RchiOBHm_Chr6g0248641 RchiOBHm_Chr6g0248651 RchiOBHm_Chr6g0248671 RchiOBHm_Chr6g0248681 RchiOBHm_Chr6g0248731 RchiOBHm_Chr6g0248741 RchiOBHm_Chr6g0248771 RchiOBHm_Chr6g0248791 RchiOBHm_Chr6g0248851 RchiOBHm_Chr6g0248871 RchiOBHm_Chr6g0248881 RchiOBHm_Chr6g0248921 RchiOBHm_Chr6g0252081 RchiOBHm_Chr6g0252091 RchiOBHm_Chr6g0252101 RchiOBHm_Chr6g0267131 RchiOBHm_Chr6g0295981 RchiOBHm_Chr6g0295991 RchiOBHm_Chr6g0296021 RchiOBHm_Chr6g0300311 RchiOBHm_Chr7g0182631
rosa_laevigata RLG00000005114 RLG00000007652 RLG00000011348 RLG00000011741 RLG00000011742 RLG00000011743 RLG00000011744 RLG00000011745 RLG00000011747 RLG00000011748 RLG00000011749 RLG00000011750 RLG00000014028 RLG00000014036 RLG00000015111 RLG00000015113 RLG00000015115 RLG00000015117 RLG00000015119 RLG00000015123 RLG00000015124 RLG00000015127 RLG00000015128 RLG00000015129 RLG00000015132 RLG00000033513 RLG00000034608
rosa_multiflora Rmu_co8062356.1_g000001 Rmu_co8494777.1_g000001 Rmu_sc0000229.1_g000003 Rmu_sc0000529.1_g000001 Rmu_sc0000529.1_g000002 Rmu_sc0000529.1_g000003 Rmu_sc0001014.1_g000001 Rmu_sc0001150.1_g000007 Rmu_sc0001562.1_g000002 Rmu_sc0001562.1_g000004 Rmu_sc0001562.1_g000005 Rmu_sc0001562.1_g000017 Rmu_sc0001562.1_g000033 Rmu_sc0001562.1_g000035 Rmu_sc0001670.1_g000022 Rmu_sc0002393.1_g000007 Rmu_sc0002393.1_g000008 Rmu_sc0002393.1_g000009 Rmu_sc0002393.1_g000010 Rmu_sc0002393.1_g000011 Rmu_sc0002393.1_g000021 Rmu_sc0002393.1_g000023 Rmu_sc0002393.1_g000035 Rmu_sc0002393.1_g000037 Rmu_sc0002489.1_g000003 Rmu_sc0002938.1_g000047 Rmu_sc0003139.1_g000013 Rmu_sc0003139.1_g000014 Rmu_sc0003139.1_g000015 Rmu_sc0003139.1_g000017 Rmu_sc0004275.1_g000033 Rmu_sc0004275.1_g000036 Rmu_sc0004703.1_g000001 Rmu_sc0006276.1_g000003 Rmu_sc0010676.1_g000003 Rmu_sc0010676.1_g000004 Rmu_sc0010676.1_g000006 Rmu_sc0010676.1_g000007 Rmu_sc0013953.1_g000004 Rmu_ssc0000388.1_g000016
rosa_roxburghii Rroxscaffold_1G00015220 Rroxscaffold_1G00046270 Rroxscaffold_2G00090150 Rroxscaffold_2G00120650 Rroxscaffold_2G00123380 Rroxscaffold_3G00234080 Rroxscaffold_3G00271110 Rroxscaffold_7G00167900 Rroxscaffold_7G00171950 Rroxscaffold_7G00171970 Rroxscaffold_7G00171980 Rroxscaffold_7G00171990 Rroxscaffold_7G00172000 Rroxscaffold_7G00201050 Rroxscaffold_7G00212860 Rroxscaffold_7G00212880 Rroxscaffold_7G00212920 Rroxscaffold_7G00212940 Rroxscaffold_7G00212990 Rroxscaffold_7G00213060 Rroxscaffold_7G00213070 Rroxscaffold_7G00213080 Rroxscaffold_7G00213100 Rroxscaffold_7G00213110 Rroxscaffold_7G00213120 Rroxscaffold_7G00213130 Rroxscaffold_7G00213140 Rroxscaffold_7G00217950
rosa_rugosa Rorug04G0089100 Rorug04G0176100 Rorug05G0143700 Rorug05G0532600 Rorug05G0532700 Rorug05G0532800 Rorug05G0533100 Rorug05G0533200 Rorug05G0533300 Rorug05G0533400 Rorug05G0533500 Rorug05G0533500 Rorug05G0533700 Rorug05G0533800 Rorug05G0533900 Rorug05G0534000 Rorug05G0534100.1 Rorug05G0534200 Rorug06G0029200 Rorug06G0029300 Rorug06G0029400 Rorug06G0255600 Rorug06G0255800 Rorug06G0255800 Rorug06G0255900 Rorug06G0255900 Rorug06G0255900 Rorug06G0256000 Rorug06G0256100 Rorug06G0256300 Rorug06G0256400 Rorug06G0294900 Rorug06G0448600 Rorug06G0448600 Rorug07G0094000
rosa_samantha Rh3BG070200 Rh3CG325900 Rh5BG235500 Rh6AG049500 Rh6AG049600 Rh6AG049800 Rh6AG049900 Rh6AG050100 Rh6AG050200 Rh6AG050300 Rh6AG050400 Rh6AG050500 Rh6AG050800 Rh6AG051100 Rh6AG051500 Rh6AG151100 Rh6AG369300 Rh6AG369500 Rh6AG369600 Rh6AG369800 Rh6AG369900 Rh6AG407600 Rh6DG038100 Rh7CG053500 Rh7CG053600 Rh7DG051800
rosa_wichuraiana Rw3G005370 Rw3G025850 Rw5G021490 Rw6G004290 Rw6G004300 Rw6G004310 Rw6G004320 Rw6G004330 Rw6G004340 Rw6G004390 Rw6G004410 Rw6G004420 Rw6G004430 Rw6G004450 Rw6G004460 Rw6G004490 Rw6G004510 Rw6G004590 Rw6G013080 Rw6G032210 Rw6G032220 Rw6G032230 Rw6G032250 Rw6G035640 Rw7G004270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1506
AasI GACNNNNNNGTC 1 cut(s) 1395
Acc36I ACCTGC 1 cut(s) 1506
AccB1I GGYRCC 3 cut(s) 34, 1013, 2120
AccB7I CCANNNNNTGG 2 cut(s) 264, 1518
AccI GTMKAC 2 cut(s) 84, 620
AccII CGCG 2 cut(s) 977, 1490
AccIII TCCGGA 1 cut(s) 1087
AciI CCGC 4 cut(s) 224, 921, 1478, 1490
AclWI GGATC 3 cut(s) 1079, 2274, 2564
AcoI YGGCCR 2 cut(s) 279, 1533
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 7 cut(s) 153, 272, 372, 489, 832, 1526, 1939
AfiI CCNNNNNNNGG 5 cut(s) 128, 264, 334, 895, 1518
AflII CTTAAG 1 cut(s) 1391
AflIII ACRYGT 2 cut(s) 682, 701
AhlI ACTAGT 1 cut(s) 2509
AjnI CCWGG 2 cut(s) 4, 2565
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 882, 914
Alw21I GWGCWC 1 cut(s) 1656
Alw26I GTCTC 2 cut(s) 1011, 2118
AlwI GGATC 3 cut(s) 1079, 2274, 2564
Ama87I CYCGRG 1 cut(s) 1370
Aor13HI TCCGGA 1 cut(s) 1087
AoxI GGCC 5 cut(s) 279, 388, 675, 1533, 2462
ApeKI GCWGC 1 cut(s) 21
ArsI GACNNNNNNTTYG 4 cut(s) 1061, 1093, 2168, 2200
AseI ATTAAT 2 cut(s) 728, 1835
Asp700I GAANNNNTTC 2 cut(s) 1029, 2136
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 4 cut(s) 389, 676, 881, 1988
AsuC2I CCSGG 1 cut(s) 2195
AsuHPI GGTGA 9 cut(s) 352, 661, 745, 874, 1320, 1504, 1852, 2051, 2427
AsuII TTCGAA 1 cut(s) 967
AvaI CYCGRG 1 cut(s) 1370
AvaII GGWCC 2 cut(s) 881, 1988
BaeGI GKGCMC 1 cut(s) 37
BamHI GGATCC 1 cut(s) 2569
BanI GGYRCC 3 cut(s) 34, 1013, 2120
BarI GAAGNNNNNNTAC 2 cut(s) 363, 395
BbsI GAAGAC 2 cut(s) 1751, 2310
Bbv12I GWGCWC 1 cut(s) 1656
BbvI GCAGC 1 cut(s) 33
BcgI CGANNNNNNTGC 6 cut(s) 395, 429, 967, 1001, 2073, 2107
BciT130I CCWGG 2 cut(s) 6, 2567
BciVI GTATCC 1 cut(s) 2283
BclI TGATCA 3 cut(s) 364, 1645, 2056
BcnI CCSGG 1 cut(s) 2195
BcoDI GTCTC 2 cut(s) 1011, 2118
BcuI ACTAGT 1 cut(s) 2509
BfmI CTRYAG 4 cut(s) 1065, 1136, 2172, 2243
BfrI CTTAAG 1 cut(s) 1391
BfuAI ACCTGC 1 cut(s) 1506
BfuI GTATCC 1 cut(s) 2283
BglII AGATCT 5 cut(s) 292, 544, 1275, 1546, 2382
BisI GCNGC 3 cut(s) 22, 224, 1478
BlsI GCNGC 3 cut(s) 23, 225, 1479
BmcAI AGTACT 1 cut(s) 153
Bme1390I CCNGG 3 cut(s) 6, 2195, 2567
Bme18I GGWCC 2 cut(s) 881, 1988
BmeT110I CYCGRG 1 cut(s) 1370
BmgT120I GGNCC 4 cut(s) 389, 676, 881, 1988
BmiI GGNNCC 9 cut(s) 36, 391, 900, 915, 1015, 2007, 2022, 2122, 2571
BmrFI CCNGG 3 cut(s) 6, 2195, 2567
BmsI GCATC 8 cut(s) 126, 675, 760, 838, 1081, 1867, 1945, 2188
BpiI GAAGAC 2 cut(s) 1751, 2310
BpmI CTGGAG 1 cut(s) 2510
Bpu10I CCTNAGC 2 cut(s) 227, 1481
Bpu14I TTCGAA 1 cut(s) 967
BpuEI CTTGAG 5 cut(s) 740, 923, 1358, 1847, 2465
BpuMI CCSGG 1 cut(s) 2195
Bsa29I ATCGAT 1 cut(s) 445
BsaAI YACGTR 1 cut(s) 370
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 2 cut(s) 5, 672
BsaWI WCCGGW 1 cut(s) 1087
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 264, 334, 895, 1518
Bse1I ACTGG 1 cut(s) 2527
Bse8I GATNNNNATC 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 1087
BseBI CCWGG 2 cut(s) 6, 2567
BseCI ATCGAT 1 cut(s) 445
BseDI CCNNGG 2 cut(s) 5, 672
BseGI GGATG 5 cut(s) 1096, 1165, 1974, 2203, 2335
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 5 cut(s) 128, 264, 334, 895, 1518
BseMII CTCAG 6 cut(s) 1109, 1189, 1263, 2059, 2216, 2370
BseNI ACTGG 1 cut(s) 2527
BseRI GAGGAG 4 cut(s) 75, 334, 1714, 2457
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 2 cut(s) 977, 1490
BshFI GGCC 5 cut(s) 281, 390, 677, 1535, 2464
BshNI GGYRCC 3 cut(s) 34, 1013, 2120
BshVI ATCGAT 1 cut(s) 445
BsiHKAI GWGCWC 1 cut(s) 1656
BsiHKCI CYCGRG 1 cut(s) 1370
BsiSI CCGG 2 cut(s) 1088, 2195
BslFI GGGAC 3 cut(s) 106, 1170, 1499
BslI CCNNNNNNNGG 5 cut(s) 128, 264, 334, 895, 1518
BsmAI GTCTC 2 cut(s) 1011, 2118
BsmFI GGGAC 3 cut(s) 106, 1170, 1499
BsmI GAATGC 2 cut(s) 1113, 2220
BsnI GGCC 5 cut(s) 281, 390, 677, 1535, 2464
BsoBI CYCGRG 1 cut(s) 1370
Bsp119I TTCGAA 1 cut(s) 967
Bsp1286I GDGCHC 2 cut(s) 37, 1656
Bsp13I TCCGGA 1 cut(s) 1087
Bsp19I CCATGG 1 cut(s) 672
Bsp68I TCGCGA 1 cut(s) 977
BspACI CCGC 4 cut(s) 224, 921, 1478, 1490
BspANI GGCC 5 cut(s) 281, 390, 677, 1535, 2464
BspCNI CTCAG 6 cut(s) 1110, 1188, 1264, 2058, 2217, 2371
BspDI ATCGAT 1 cut(s) 445
BspEI TCCGGA 1 cut(s) 1087
BspFNI CGCG 2 cut(s) 977, 1490
BspHI TCATGA 1 cut(s) 1360
BspLI GGNNCC 9 cut(s) 36, 391, 900, 915, 1015, 2007, 2022, 2122, 2571
BspMAI CTGCAG 1 cut(s) 1140
BspMI ACCTGC 1 cut(s) 1506
BspPI GGATC 3 cut(s) 1079, 2274, 2564
BspQI GCTCTTC 1 cut(s) 641
BspT104I TTCGAA 1 cut(s) 967
BspT107I GGYRCC 3 cut(s) 34, 1013, 2120
BspTI CTTAAG 1 cut(s) 1391
BsrI ACTGG 1 cut(s) 2527
BssECI CCNNGG 2 cut(s) 5, 672
BssT1I CCWWGG 1 cut(s) 672
Bst2UI CCWGG 2 cut(s) 6, 2567
Bst4CI ACNGT 4 cut(s) 75, 583, 1351, 2491
Bst6I CTCTTC 2 cut(s) 641, 1748
BstAFI CTTAAG 1 cut(s) 1391
BstBAI YACGTR 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 967
BstC8I GCNNGC 4 cut(s) 975, 2082, 2484, 2550
BstDSI CCRYGG 1 cut(s) 672
BstF5I GGATG 5 cut(s) 1096, 1165, 1974, 2203, 2335
BstFNI CGCG 2 cut(s) 977, 1490
BstHHI GCGC 1 cut(s) 775
BstMAI GTCTC 2 cut(s) 1011, 2118
BstMWI GCNNNNNNNGC 9 cut(s) 145, 396, 648, 927, 1236, 2034, 2134, 2343, 2492
BstNI CCWGG 2 cut(s) 6, 2567
BstNSI RCATGY 2 cut(s) 686, 2552
BstSCI CCNGG 3 cut(s) 4, 2193, 2565
BstSFI CTRYAG 4 cut(s) 1065, 1136, 2172, 2243
BstSLI GKGCMC 1 cut(s) 37
BstUI CGCG 2 cut(s) 977, 1490
BstV1I GCAGC 1 cut(s) 33
BstV2I GAAGAC 2 cut(s) 1751, 2310
BstX2I RGATCY 6 cut(s) 292, 544, 1275, 1546, 2382, 2569
BstXI CCANNNNNNTGG 1 cut(s) 1985
BstYI RGATCY 6 cut(s) 292, 544, 1275, 1546, 2382, 2569
Bsu15I ATCGAT 1 cut(s) 445
BsuI GTATCC 1 cut(s) 2283
BsuRI GGCC 5 cut(s) 281, 390, 677, 1535, 2464
BsuTUI ATCGAT 1 cut(s) 445
BtgI CCRYGG 1 cut(s) 672
BtsCI GGATG 5 cut(s) 1096, 1165, 1974, 2203, 2335
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
BtuMI TCGCGA 1 cut(s) 977
BveI ACCTGC 1 cut(s) 1506
Cac8I GCNNGC 4 cut(s) 975, 2082, 2484, 2550
CciI TCATGA 1 cut(s) 1360
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 4 cut(s) 389, 676, 881, 1988
ClaI ATCGAT 1 cut(s) 445
CseI GACGC 1 cut(s) 1496
Csp6I GTAC 7 cut(s) 152, 271, 371, 488, 831, 1525, 1938
CviQI GTAC 7 cut(s) 152, 271, 371, 488, 831, 1525, 1938
DraI TTTAAA 4 cut(s) 594, 1221, 2103, 2533
DrdI GACNNNNNNGTC 1 cut(s) 1395
DseDI GACNNNNNNGTC 1 cut(s) 1395
EaeI YGGCCR 2 cut(s) 279, 1533
Eam1104I CTCTTC 2 cut(s) 641, 1748
EarI CTCTTC 2 cut(s) 641, 1748
Eco130I CCWWGG 1 cut(s) 672
Eco147I AGGCCT 1 cut(s) 2464
Eco47I GGWCC 2 cut(s) 881, 1988
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 1370
EcoRII CCWGG 2 cut(s) 4, 2565
EcoT14I CCWWGG 1 cut(s) 672
EcoT22I ATGCAT 1 cut(s) 2222
ErhI CCWWGG 1 cut(s) 672
FalI AAGNNNNNCTT 2 cut(s) 2081, 2113
FaqI GGGAC 3 cut(s) 106, 1170, 1499
FauNDI CATATG 2 cut(s) 1073, 2180
FbaI TGATCA 3 cut(s) 364, 1645, 2056
FblI GTMKAC 2 cut(s) 84, 620
Fnu4HI GCNGC 3 cut(s) 22, 224, 1478
FokI GGATG 5 cut(s) 1103, 1172, 1961, 2210, 2342
Fsp4HI GCNGC 3 cut(s) 22, 224, 1478
GlaI GCGC 1 cut(s) 774
GluI GCNGC 3 cut(s) 22, 224, 1478
GsuI CTGGAG 1 cut(s) 2510
HaeIII GGCC 5 cut(s) 281, 390, 677, 1535, 2464
HapII CCGG 2 cut(s) 1088, 2195
HgaI GACGC 1 cut(s) 1496
HhaI GCGC 1 cut(s) 775
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HincII GTYRAC 3 cut(s) 564, 621, 1777
HindII GTYRAC 3 cut(s) 564, 621, 1777
HindIII AAGCTT 2 cut(s) 1028, 2135
HinfI GANTC 7 cut(s) 86, 633, 1183, 1396, 1756, 1807, 2540
HpaI GTTAAC 1 cut(s) 564
HpaII CCGG 2 cut(s) 1088, 2195
HphI GGTGA 9 cut(s) 352, 661, 745, 874, 1320, 1504, 1852, 2051, 2427
Hpy99I CGWCG 2 cut(s) 622, 625
HpyAV CCTTC 3 cut(s) 662, 963, 1173
HpyCH4III ACNGT 4 cut(s) 75, 583, 1351, 2491
HpyCH4IV ACGT 6 cut(s) 369, 623, 703, 1471, 1779, 2536
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 396, 648, 927, 1236, 2034, 2134, 2343, 2492
HpySE526I ACGT 6 cut(s) 369, 623, 703, 1471, 1779, 2536
HspAI GCGC 1 cut(s) 773
Kpn2I TCCGGA 1 cut(s) 1087
Ksp22I TGATCA 3 cut(s) 364, 1645, 2056
KspAI GTTAAC 1 cut(s) 564
LguI GCTCTTC 1 cut(s) 641
LmnI GCTCC 3 cut(s) 639, 1130, 2237
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 8 cut(s) 126, 675, 760, 838, 1081, 1867, 1945, 2188
MaeII ACGT 6 cut(s) 369, 623, 703, 1471, 1779, 2536
MaeIII GTNAC 9 cut(s) 49, 196, 340, 751, 1351, 1450, 1467, 1858, 1969
MboII GAAGA 7 cut(s) 169, 658, 1203, 1211, 1751, 1765, 2310
MfeI CAATTG 2 cut(s) 1234, 2341
MflI RGATCY 6 cut(s) 292, 544, 1275, 1546, 2382, 2569
MhlI GDGCHC 2 cut(s) 37, 1656
MlyI GAGTC 4 cut(s) 80, 1192, 1405, 1765
MmeI TCCRAC 1 cut(s) 147
Mph1103I ATGCAT 1 cut(s) 2222
MroI TCCGGA 1 cut(s) 1087
MroXI GAANNNNTTC 2 cut(s) 1029, 2136
MslI CAYNNNNRTG 3 cut(s) 671, 744, 1983
MspCI CTTAAG 1 cut(s) 1391
MspI CCGG 2 cut(s) 1088, 2195
MspR9I CCNGG 3 cut(s) 6, 2195, 2567
MssI GTTTAAAC 1 cut(s) 2533
MunI CAATTG 2 cut(s) 1234, 2341
Mva1269I GAATGC 2 cut(s) 1113, 2220
MvaI CCWGG 2 cut(s) 6, 2567
MvnI CGCG 2 cut(s) 977, 1490
MwoI GCNNNNNNNGC 9 cut(s) 145, 396, 648, 927, 1236, 2034, 2134, 2343, 2492
NciI CCSGG 1 cut(s) 2195
NcoI CCATGG 1 cut(s) 672
NdeI CATATG 2 cut(s) 1073, 2180
NlaIV GGNNCC 9 cut(s) 36, 391, 900, 915, 1015, 2007, 2022, 2122, 2571
NmuCI GTSAC 8 cut(s) 196, 340, 751, 1351, 1450, 1467, 1858, 1969
NruI TCGCGA 1 cut(s) 977
NsiI ATGCAT 1 cut(s) 2222
NspI RCATGY 2 cut(s) 686, 2552
NspV TTCGAA 1 cut(s) 967
PaeI GCATGC 1 cut(s) 2552
PagI TCATGA 1 cut(s) 1360
PaqCI CACCTGC 1 cut(s) 1506
PceI AGGCCT 1 cut(s) 2464
PciI ACATGT 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 641
PcsI WCGNNNNNNNCGW 1 cut(s) 1776
PctI GAATGC 2 cut(s) 1113, 2220
PdmI GAANNNNTTC 2 cut(s) 1029, 2136
PfeI GAWTC 3 cut(s) 633, 1807, 2540
PflMI CCANNNNNTGG 2 cut(s) 264, 1518
PfoI TCCNGGA 1 cut(s) 2565
PkrI GCNGC 3 cut(s) 23, 225, 1479
PleI GAGTC 4 cut(s) 80, 1191, 1404, 1764
PmeI GTTTAAAC 1 cut(s) 2533
PpsI GAGTC 4 cut(s) 80, 1191, 1404, 1764
Ppu21I YACGTR 1 cut(s) 370
PscI ACATGT 1 cut(s) 682
PshBI ATTAAT 2 cut(s) 728, 1835
Psp6I CCWGG 2 cut(s) 4, 2565
PspGI CCWGG 2 cut(s) 4, 2565
PspN4I GGNNCC 9 cut(s) 36, 391, 900, 915, 1015, 2007, 2022, 2122, 2571
PspPI GGNCC 4 cut(s) 389, 676, 881, 1988
PstI CTGCAG 1 cut(s) 1140
PsuI RGATCY 6 cut(s) 292, 544, 1275, 1546, 2382, 2569
RruI TCGCGA 1 cut(s) 977
RsaI GTAC 7 cut(s) 153, 272, 372, 489, 832, 1526, 1939
RsaNI GTAC 7 cut(s) 152, 271, 371, 488, 831, 1525, 1938
RseI CAYNNNNRTG 3 cut(s) 671, 744, 1983
SalI GTCGAC 1 cut(s) 619
SapI GCTCTTC 1 cut(s) 641
SatI GCNGC 3 cut(s) 22, 224, 1478
Sau96I GGNCC 4 cut(s) 389, 676, 881, 1988
ScaI AGTACT 1 cut(s) 153
SchI GAGTC 4 cut(s) 80, 1192, 1405, 1765
ScrFI CCNGG 3 cut(s) 6, 2195, 2567
SduI GDGCHC 2 cut(s) 37, 1656
SfaNI GCATC 8 cut(s) 126, 675, 760, 838, 1081, 1867, 1945, 2188
SfcI CTRYAG 4 cut(s) 1065, 1136, 2172, 2243
SfuI TTCGAA 1 cut(s) 967
SgrDI CGTCGACG 1 cut(s) 619
SinI GGWCC 2 cut(s) 881, 1988
SmiMI CAYNNNNRTG 3 cut(s) 671, 744, 1983
SmlI CTYRAG 6 cut(s) 719, 938, 1337, 1391, 1826, 2444
SmoI CTYRAG 6 cut(s) 719, 938, 1337, 1391, 1826, 2444
SpeI ACTAGT 1 cut(s) 2509
SphI GCATGC 1 cut(s) 2552
SseBI AGGCCT 1 cut(s) 2464
SsiI CCGC 4 cut(s) 224, 921, 1478, 1490
SspI AATATT 5 cut(s) 732, 766, 1839, 1873, 2071
StuI AGGCCT 1 cut(s) 2464
StyD4I CCNGG 3 cut(s) 4, 2193, 2565
StyI CCWWGG 1 cut(s) 672
TaaI ACNGT 4 cut(s) 75, 583, 1351, 2491
TaiI ACGT 6 cut(s) 372, 626, 706, 1474, 1782, 2539
TaqI TCGA 9 cut(s) 134, 405, 445, 473, 620, 967, 1197, 1588, 2470
TatI WGTACW 1 cut(s) 151
TauI GCSGC 2 cut(s) 226, 1480
TfiI GAWTC 3 cut(s) 633, 1807, 2540
TscAI CASTG 1 cut(s) 243
TseFI GTSAC 8 cut(s) 196, 340, 751, 1351, 1450, 1467, 1858, 1969
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 8 cut(s) 196, 340, 751, 1351, 1450, 1467, 1858, 1969
TspDTI ATGAA 6 cut(s) 371, 566, 1294, 1377, 1652, 2401
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 1 cut(s) 243
Van91I CCANNNNNTGG 2 cut(s) 264, 1518
Vha464I CTTAAG 1 cut(s) 1391
VpaK11BI GGWCC 2 cut(s) 881, 1988
VspI ATTAAT 2 cut(s) 728, 1835
XbaI TCTAGA 1 cut(s) 1549
XceI RCATGY 2 cut(s) 686, 2552
XmiI GTMKAC 2 cut(s) 84, 620
XmnI GAANNNNTTC 2 cut(s) 1029, 2136
ZrmI AGTACT 1 cut(s) 153
Zsp2I ATGCAT 1 cut(s) 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.