Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 135, 192, 458 |
| AcuI | CTGAAG | 1 cut(s) | 172 |
| AgsI | TTSAA | 1 cut(s) | 394 |
| AjnI | CCWGG | 1 cut(s) | 179 |
| AluBI | AGCT | 2 cut(s) | 186, 235 |
| AluI | AGCT | 2 cut(s) | 186, 235 |
| AoxI | GGCC | 1 cut(s) | 75 |
| ApeKI | GCWGC | 2 cut(s) | 113, 232 |
| AspLEI | GCGC | 1 cut(s) | 42 |
| AsuHPI | GGTGA | 1 cut(s) | 45 |
| AsuII | TTCGAA | 1 cut(s) | 209 |
| BbsI | GAAGAC | 1 cut(s) | 328 |
| BbvI | GCAGC | 2 cut(s) | 100, 244 |
| BccI | CCATC | 2 cut(s) | 358, 391 |
| BciT130I | CCWGG | 1 cut(s) | 181 |
| BfaI | CTAG | 1 cut(s) | 21 |
| BisI | GCNGC | 3 cut(s) | 114, 193, 233 |
| BlsI | GCNGC | 3 cut(s) | 115, 194, 234 |
| Bme1390I | CCNGG | 1 cut(s) | 181 |
| BmrFI | CCNGG | 1 cut(s) | 181 |
| BmsI | GCATC | 3 cut(s) | 27, 259, 297 |
| BpiI | GAAGAC | 1 cut(s) | 328 |
| BpmI | CTGGAG | 1 cut(s) | 187 |
| Bpu14I | TTCGAA | 1 cut(s) | 209 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 117, 179 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 367, 397 |
| Bse1I | ACTGG | 1 cut(s) | 204 |
| BseBI | CCWGG | 1 cut(s) | 181 |
| BseDI | CCNNGG | 3 cut(s) | 72, 117, 179 |
| BseGI | GGATG | 2 cut(s) | 369, 375 |
| BseNI | ACTGG | 1 cut(s) | 204 |
| BseRI | GAGGAG | 2 cut(s) | 190, 388 |
| BseXI | GCAGC | 2 cut(s) | 100, 244 |
| BseYI | CCCAGC | 1 cut(s) | 506 |
| BshFI | GGCC | 1 cut(s) | 77 |
| BslFI | GGGAC | 1 cut(s) | 296 |
| BsmFI | GGGAC | 1 cut(s) | 296 |
| BsnI | GGCC | 1 cut(s) | 77 |
| Bsp119I | TTCGAA | 1 cut(s) | 209 |
| BspACI | CCGC | 3 cut(s) | 135, 192, 458 |
| BspANI | GGCC | 1 cut(s) | 77 |
| BspT104I | TTCGAA | 1 cut(s) | 209 |
| BsrI | ACTGG | 1 cut(s) | 204 |
| BssECI | CCNNGG | 3 cut(s) | 72, 117, 179 |
| BssT1I | CCWWGG | 2 cut(s) | 72, 117 |
| Bst2UI | CCWGG | 1 cut(s) | 181 |
| Bst4CI | ACNGT | 1 cut(s) | 383 |
| Bst6I | CTCTTC | 2 cut(s) | 89, 310 |
| BstBI | TTCGAA | 1 cut(s) | 209 |
| BstDEI | CTNAG | 1 cut(s) | 239 |
| BstF5I | GGATG | 2 cut(s) | 369, 375 |
| BstHHI | GCGC | 1 cut(s) | 42 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 192 |
| BstNI | CCWGG | 1 cut(s) | 181 |
| BstSCI | CCNGG | 1 cut(s) | 179 |
| BstV1I | GCAGC | 2 cut(s) | 100, 244 |
| BstV2I | GAAGAC | 1 cut(s) | 328 |
| BsuRI | GGCC | 1 cut(s) | 77 |
| BtsCI | GGATG | 2 cut(s) | 369, 375 |
| BtsI | GCAGTG | 1 cut(s) | 153 |
| BtsIMutI | CAGTG | 2 cut(s) | 153, 388 |
| CfoI | GCGC | 1 cut(s) | 42 |
| CseI | GACGC | 1 cut(s) | 237 |
| CviAII | CATG | 1 cut(s) | 91 |
| CviJI | RGCY | 4 cut(s) | 77, 186, 235, 422 |
| CviKI_1 | RGCY | 4 cut(s) | 77, 186, 235, 422 |
| DdeI | CTNAG | 1 cut(s) | 239 |
| Eam1104I | CTCTTC | 2 cut(s) | 89, 310 |
| EarI | CTCTTC | 2 cut(s) | 89, 310 |
| EciI | GGCGGA | 1 cut(s) | 473 |
| Eco130I | CCWWGG | 2 cut(s) | 72, 117 |
| Eco57I | CTGAAG | 1 cut(s) | 172 |
| EcoRII | CCWGG | 1 cut(s) | 179 |
| EcoT14I | CCWWGG | 2 cut(s) | 72, 117 |
| ErhI | CCWWGG | 2 cut(s) | 72, 117 |
| FaeI | CATG | 1 cut(s) | 94 |
| FaiI | YATR | 5 cut(s) | 92, 129, 468, 486, 517 |
| FaqI | GGGAC | 1 cut(s) | 296 |
| FatI | CATG | 1 cut(s) | 90 |
| Fnu4HI | GCNGC | 3 cut(s) | 114, 193, 233 |
| FokI | GGATG | 2 cut(s) | 376, 382 |
| Fsp4HI | GCNGC | 3 cut(s) | 114, 193, 233 |
| FspBI | CTAG | 1 cut(s) | 21 |
| GlaI | GCGC | 1 cut(s) | 41 |
| GluI | GCNGC | 3 cut(s) | 114, 193, 233 |
| GsaI | CCCAGC | 1 cut(s) | 510 |
| GsuI | CTGGAG | 1 cut(s) | 187 |
| HaeIII | GGCC | 1 cut(s) | 77 |
| HgaI | GACGC | 1 cut(s) | 237 |
| HhaI | GCGC | 1 cut(s) | 42 |
| Hin1II | CATG | 1 cut(s) | 94 |
| Hin6I | GCGC | 1 cut(s) | 40 |
| HinP1I | GCGC | 1 cut(s) | 40 |
| HincII | GTYRAC | 1 cut(s) | 493 |
| HindII | GTYRAC | 1 cut(s) | 493 |
| HinfI | GANTC | 1 cut(s) | 64 |
| HpaI | GTTAAC | 1 cut(s) | 493 |
| HphI | GGTGA | 1 cut(s) | 45 |
| Hpy166II | GTNNAC | 1 cut(s) | 493 |
| Hpy188I | TCNGA | 1 cut(s) | 304 |
| Hpy8I | GTNNAC | 1 cut(s) | 493 |
| Hpy99I | CGWCG | 1 cut(s) | 253 |
| HpyAV | CCTTC | 2 cut(s) | 396, 448 |
| HpyCH4III | ACNGT | 1 cut(s) | 383 |
| HpyCH4V | TGCA | 2 cut(s) | 272, 310 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 192 |
| HpyF3I | CTNAG | 1 cut(s) | 239 |
| Hsp92II | CATG | 1 cut(s) | 94 |
| HspAI | GCGC | 1 cut(s) | 40 |
| KspAI | GTTAAC | 1 cut(s) | 493 |
| LmnI | GCTCC | 1 cut(s) | 183 |
| LpnPI | CCDG | 5 cut(s) | 166, 193, 217, 282, 492 |
| Lsp1109I | GCAGC | 2 cut(s) | 100, 244 |
| LweI | GCATC | 3 cut(s) | 27, 259, 297 |
| MaeI | CTAG | 1 cut(s) | 21 |
| MaeIII | GTNAC | 2 cut(s) | 149, 377 |
| MboII | GAAGA | 8 cut(s) | 42, 106, 116, 271, 327, 333, 406, 449 |
| MlyI | GAGTC | 1 cut(s) | 58 |
| MmeI | TCCRAC | 1 cut(s) | 367 |
| MnlI | CCTC | 7 cut(s) | 211, 214, 222, 311, 366, 406, 427 |
| MseI | TTAA | 1 cut(s) | 492 |
| MspA1I | CMGCKG | 1 cut(s) | 192 |
| MspR9I | CCNGG | 1 cut(s) | 181 |
| MvaI | CCWGG | 1 cut(s) | 181 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 192 |
| NlaIII | CATG | 1 cut(s) | 94 |
| NspV | TTCGAA | 1 cut(s) | 209 |
| PkrI | GCNGC | 3 cut(s) | 115, 194, 234 |
| PleI | GAGTC | 1 cut(s) | 58 |
| PpsI | GAGTC | 1 cut(s) | 58 |
| Psp6I | CCWGG | 1 cut(s) | 179 |
| PspFI | CCCAGC | 1 cut(s) | 506 |
| PspGI | CCWGG | 1 cut(s) | 179 |
| SaqAI | TTAA | 1 cut(s) | 492 |
| SatI | GCNGC | 3 cut(s) | 114, 193, 233 |
| SchI | GAGTC | 1 cut(s) | 58 |
| ScrFI | CCNGG | 1 cut(s) | 181 |
| SetI | ASST | 4 cut(s) | 123, 188, 237, 294 |
| SfaNI | GCATC | 3 cut(s) | 27, 259, 297 |
| SfuI | TTCGAA | 1 cut(s) | 209 |
| SsiI | CCGC | 3 cut(s) | 135, 192, 458 |
| SspMI | CTAG | 1 cut(s) | 21 |
| StyD4I | CCNGG | 1 cut(s) | 179 |
| StyI | CCWWGG | 2 cut(s) | 72, 117 |
| TaaI | ACNGT | 1 cut(s) | 383 |
| TaqI | TCGA | 3 cut(s) | 209, 425, 447 |
| TauI | GCSGC | 1 cut(s) | 195 |
| Tru1I | TTAA | 1 cut(s) | 492 |
| Tru9I | TTAA | 1 cut(s) | 492 |
| TscAI | CASTG | 2 cut(s) | 160, 388 |
| TseI | GCWGC | 2 cut(s) | 113, 232 |
| TspDTI | ATGAA | 3 cut(s) | 107, 368, 456 |
| TspRI | CASTG | 2 cut(s) | 160, 388 |
| XspI | CTAG | 1 cut(s) | 21 |