Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 293 |
| AciI | CCGC | 6 cut(s) | 132, 135, 138, 141, 144, 464 |
| AcuI | CTGAAG | 1 cut(s) | 178 |
| AgsI | TTSAA | 1 cut(s) | 400 |
| AluBI | AGCT | 2 cut(s) | 192, 241 |
| AluI | AGCT | 2 cut(s) | 192, 241 |
| AoxI | GGCC | 1 cut(s) | 75 |
| ApeKI | GCWGC | 3 cut(s) | 113, 238, 299 |
| AspLEI | GCGC | 1 cut(s) | 42 |
| AsuHPI | GGTGA | 1 cut(s) | 45 |
| AsuII | TTCGAA | 1 cut(s) | 215 |
| BbsI | GAAGAC | 1 cut(s) | 334 |
| BbvI | GCAGC | 3 cut(s) | 100, 250, 311 |
| BccI | CCATC | 2 cut(s) | 364, 397 |
| BceAI | ACGGC | 2 cut(s) | 145, 267 |
| BfaI | CTAG | 1 cut(s) | 21 |
| BfuAI | ACCTGC | 1 cut(s) | 293 |
| BisI | GCNGC | 8 cut(s) | 114, 133, 136, 139, 142, 145, 239, 300 |
| BlsI | GCNGC | 8 cut(s) | 115, 134, 137, 140, 143, 146, 240, 301 |
| BmsI | GCATC | 2 cut(s) | 27, 263 |
| BpiI | GAAGAC | 1 cut(s) | 334 |
| BpmI | CTGGAG | 1 cut(s) | 193 |
| Bpu14I | TTCGAA | 1 cut(s) | 215 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 117, 185 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 373, 403 |
| Bse1I | ACTGG | 1 cut(s) | 210 |
| BseDI | CCNNGG | 3 cut(s) | 72, 117, 185 |
| BseGI | GGATG | 2 cut(s) | 375, 381 |
| BseNI | ACTGG | 1 cut(s) | 210 |
| BseRI | GAGGAG | 3 cut(s) | 109, 196, 394 |
| BseXI | GCAGC | 3 cut(s) | 100, 250, 311 |
| BshFI | GGCC | 1 cut(s) | 77 |
| BslFI | GGGAC | 1 cut(s) | 302 |
| BsmFI | GGGAC | 1 cut(s) | 302 |
| BsnI | GGCC | 1 cut(s) | 77 |
| Bsp119I | TTCGAA | 1 cut(s) | 215 |
| BspACI | CCGC | 6 cut(s) | 132, 135, 138, 141, 144, 464 |
| BspANI | GGCC | 1 cut(s) | 77 |
| BspMI | ACCTGC | 1 cut(s) | 293 |
| BspT104I | TTCGAA | 1 cut(s) | 215 |
| BsrI | ACTGG | 1 cut(s) | 210 |
| BssECI | CCNNGG | 3 cut(s) | 72, 117, 185 |
| BssT1I | CCWWGG | 3 cut(s) | 72, 117, 185 |
| Bst4CI | ACNGT | 1 cut(s) | 180 |
| Bst6I | CTCTTC | 1 cut(s) | 316 |
| BstBI | TTCGAA | 1 cut(s) | 215 |
| BstDEI | CTNAG | 1 cut(s) | 245 |
| BstF5I | GGATG | 2 cut(s) | 375, 381 |
| BstHHI | GCGC | 1 cut(s) | 42 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 138, 141, 144 |
| BstV1I | GCAGC | 3 cut(s) | 100, 250, 311 |
| BstV2I | GAAGAC | 1 cut(s) | 334 |
| BsuRI | GGCC | 1 cut(s) | 77 |
| BtsCI | GGATG | 2 cut(s) | 375, 381 |
| BtsI | GCAGTG | 1 cut(s) | 159 |
| BtsIMutI | CAGTG | 1 cut(s) | 159 |
| BveI | ACCTGC | 1 cut(s) | 293 |
| CfoI | GCGC | 1 cut(s) | 42 |
| CseI | GACGC | 1 cut(s) | 187 |
| CviAII | CATG | 1 cut(s) | 91 |
| CviJI | RGCY | 4 cut(s) | 77, 192, 241, 428 |
| CviKI_1 | RGCY | 4 cut(s) | 77, 192, 241, 428 |
| DdeI | CTNAG | 1 cut(s) | 245 |
| Eam1104I | CTCTTC | 1 cut(s) | 316 |
| EarI | CTCTTC | 1 cut(s) | 316 |
| EciI | GGCGGA | 1 cut(s) | 479 |
| Eco130I | CCWWGG | 3 cut(s) | 72, 117, 185 |
| Eco57I | CTGAAG | 1 cut(s) | 178 |
| EcoT14I | CCWWGG | 3 cut(s) | 72, 117, 185 |
| ErhI | CCWWGG | 3 cut(s) | 72, 117, 185 |
| FaeI | CATG | 1 cut(s) | 94 |
| FaiI | YATR | 6 cut(s) | 92, 314, 387, 474, 492, 523 |
| FaqI | GGGAC | 1 cut(s) | 302 |
| FatI | CATG | 1 cut(s) | 90 |
| Fnu4HI | GCNGC | 8 cut(s) | 114, 133, 136, 139, 142, 145, 239, 300 |
| FokI | GGATG | 2 cut(s) | 382, 388 |
| Fsp4HI | GCNGC | 8 cut(s) | 114, 133, 136, 139, 142, 145, 239, 300 |
| FspBI | CTAG | 1 cut(s) | 21 |
| GlaI | GCGC | 1 cut(s) | 41 |
| GluI | GCNGC | 8 cut(s) | 114, 133, 136, 139, 142, 145, 239, 300 |
| GsuI | CTGGAG | 1 cut(s) | 193 |
| HaeIII | GGCC | 1 cut(s) | 77 |
| HgaI | GACGC | 1 cut(s) | 187 |
| HhaI | GCGC | 1 cut(s) | 42 |
| Hin1II | CATG | 1 cut(s) | 94 |
| Hin6I | GCGC | 1 cut(s) | 40 |
| HinP1I | GCGC | 1 cut(s) | 40 |
| HincII | GTYRAC | 2 cut(s) | 202, 499 |
| HindII | GTYRAC | 2 cut(s) | 202, 499 |
| HinfI | GANTC | 1 cut(s) | 64 |
| HpaI | GTTAAC | 1 cut(s) | 499 |
| HphI | GGTGA | 1 cut(s) | 45 |
| Hpy166II | GTNNAC | 2 cut(s) | 202, 499 |
| Hpy188I | TCNGA | 1 cut(s) | 310 |
| Hpy8I | GTNNAC | 2 cut(s) | 202, 499 |
| HpyAV | CCTTC | 2 cut(s) | 402, 454 |
| HpyCH4III | ACNGT | 1 cut(s) | 180 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 138, 141, 144 |
| HpyF3I | CTNAG | 1 cut(s) | 245 |
| Hsp92II | CATG | 1 cut(s) | 94 |
| HspAI | GCGC | 1 cut(s) | 40 |
| KspAI | GTTAAC | 1 cut(s) | 499 |
| LmnI | GCTCC | 1 cut(s) | 189 |
| LpnPI | CCDG | 3 cut(s) | 223, 288, 498 |
| Lsp1109I | GCAGC | 3 cut(s) | 100, 250, 311 |
| LweI | GCATC | 2 cut(s) | 27, 263 |
| MaeI | CTAG | 1 cut(s) | 21 |
| MaeIII | GTNAC | 2 cut(s) | 125, 155 |
| MboII | GAAGA | 7 cut(s) | 42, 116, 277, 333, 339, 412, 455 |
| MlyI | GAGTC | 1 cut(s) | 58 |
| MmeI | TCCRAC | 1 cut(s) | 373 |
| MnlI | CCTC | 8 cut(s) | 87, 217, 220, 228, 317, 372, 412, 433 |
| MseI | TTAA | 2 cut(s) | 345, 498 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 138, 141, 144 |
| NlaIII | CATG | 1 cut(s) | 94 |
| NspV | TTCGAA | 1 cut(s) | 215 |
| PkrI | GCNGC | 8 cut(s) | 115, 134, 137, 140, 143, 146, 240, 301 |
| PleI | GAGTC | 1 cut(s) | 58 |
| PpsI | GAGTC | 1 cut(s) | 58 |
| SaqAI | TTAA | 2 cut(s) | 345, 498 |
| SatI | GCNGC | 8 cut(s) | 114, 133, 136, 139, 142, 145, 239, 300 |
| SchI | GAGTC | 1 cut(s) | 58 |
| SetI | ASST | 4 cut(s) | 123, 194, 243, 307 |
| SfaNI | GCATC | 2 cut(s) | 27, 263 |
| SfuI | TTCGAA | 1 cut(s) | 215 |
| SsiI | CCGC | 6 cut(s) | 132, 135, 138, 141, 144, 464 |
| SspMI | CTAG | 1 cut(s) | 21 |
| StyI | CCWWGG | 3 cut(s) | 72, 117, 185 |
| TaaI | ACNGT | 1 cut(s) | 180 |
| TaqI | TCGA | 3 cut(s) | 215, 431, 453 |
| TauI | GCSGC | 5 cut(s) | 135, 138, 141, 144, 147 |
| Tru1I | TTAA | 2 cut(s) | 345, 498 |
| Tru9I | TTAA | 2 cut(s) | 345, 498 |
| TscAI | CASTG | 1 cut(s) | 166 |
| TseI | GCWGC | 3 cut(s) | 113, 238, 299 |
| TspDTI | ATGAA | 4 cut(s) | 329, 374, 462, 473 |
| TspRI | CASTG | 1 cut(s) | 166 |
| XspI | CTAG | 1 cut(s) | 21 |